SG11201810209VA - Method of amplifying circular dna - Google Patents

Method of amplifying circular dna

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Publication number
SG11201810209VA
SG11201810209VA SG11201810209VA SG11201810209VA SG11201810209VA SG 11201810209V A SG11201810209V A SG 11201810209VA SG 11201810209V A SG11201810209V A SG 11201810209VA SG 11201810209V A SG11201810209V A SG 11201810209VA SG 11201810209V A SG11201810209V A SG 11201810209VA
Authority
SG
Singapore
Prior art keywords
circular dna
catalyzes
amplifying
circular
enzyme group
Prior art date
Application number
SG11201810209VA
Inventor
Masayuki Su'etsugu
Hiroko Tsujimoto
Takeshi Shinohara
Original Assignee
Japan Science & Tech Agency
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Publication date
Application filed by Japan Science & Tech Agency filed Critical Japan Science & Tech Agency
Publication of SG11201810209VA publication Critical patent/SG11201810209VA/en

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract

METHOD OF AMPLIFYING CIRCULAR DNA Provided is a method capable of simply and exponentially amplifying circular DNA, and particularly, long-chain circular DNA, in a cell-free system. Specifically, provided herein is a method for amplifying circular DNA which comprises mixing circular DNA having a replication origin sequence (origin of chromosome (oriC)) with a reaction solution comprising: a first enzyme group that catalyzes replication of circular DNA; a second enzyme group that catalyzes an Okazaki fragment maturation and synthesizes two sister circular DNAs constituting a catenane; a third enzyme group that catalyzes a separation of two sister circular DNAs; and also, a buffer, NTP, dNTP, a magnesium ion source, and an alkali metal ion source, to form a reaction mixture, which is then reacted.
SG11201810209VA 2016-05-17 2017-05-17 Method of amplifying circular dna SG11201810209VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016099157 2016-05-17
PCT/JP2017/018472 WO2017199991A1 (en) 2016-05-17 2017-05-17 Method for amplifying cyclic dna

Publications (1)

Publication Number Publication Date
SG11201810209VA true SG11201810209VA (en) 2018-12-28

Family

ID=60325215

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Application Number Title Priority Date Filing Date
SG11201810209VA SG11201810209VA (en) 2016-05-17 2017-05-17 Method of amplifying circular dna

Country Status (11)

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US (1) US20190276883A1 (en)
EP (1) EP3460058B1 (en)
JP (1) JP6764193B2 (en)
KR (1) KR102378346B1 (en)
CN (2) CN115537454A (en)
AU (1) AU2017265723B2 (en)
CA (1) CA3024546A1 (en)
IL (1) IL263027A (en)
RU (1) RU2748736C2 (en)
SG (1) SG11201810209VA (en)
WO (1) WO2017199991A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10301672B2 (en) 2014-11-18 2019-05-28 Japan Science And Technology Agency Method of amplifying circular DNA
CN110446783A (en) * 2017-02-28 2019-11-12 奥利希罗基因组学有限公司 The duplication of cyclic DNA or amplification method
CN112424351A (en) * 2018-07-30 2021-02-26 奥利希罗基因组学有限公司 Method for editing DNA in cell-free system
US20220025460A1 (en) * 2020-07-21 2022-01-27 The University Of Tokyo Method and kit for determining neuromuscular disease in subject
CN114231524B (en) * 2020-09-09 2023-12-26 深圳华大生命科学研究院 Method for preparing circular DNA in vitro
WO2023191034A1 (en) * 2022-03-31 2023-10-05 モデルナ・エンザイマティクス株式会社 Method for producing double-stranded dna molecules having reduced sequence errors

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4214230B2 (en) 2004-02-23 2009-01-28 独立行政法人農業・食品産業技術総合研究機構 Method for introducing random mutation into circular DNA
US20080096258A1 (en) 2006-10-24 2008-04-24 Christian Korfhage Rolling circle amplification of circular genomes
US8921072B2 (en) 2008-09-02 2014-12-30 General Electric Compnay Methods to generate DNA mini-circles
CA2877368C (en) * 2012-06-29 2021-07-13 General Electric Company Kit for isothermal dna amplification starting from an rna template
US10301672B2 (en) * 2014-11-18 2019-05-28 Japan Science And Technology Agency Method of amplifying circular DNA

Also Published As

Publication number Publication date
EP3460058B1 (en) 2024-03-06
JPWO2017199991A1 (en) 2019-03-22
CN109415718B (en) 2022-09-27
US20190276883A1 (en) 2019-09-12
EP3460058A4 (en) 2020-01-15
RU2018144299A3 (en) 2020-06-17
KR20190017793A (en) 2019-02-20
JP6764193B2 (en) 2020-09-30
WO2017199991A1 (en) 2017-11-23
CN109415718A (en) 2019-03-01
RU2748736C2 (en) 2021-05-31
IL263027A (en) 2018-12-31
CA3024546A1 (en) 2017-11-23
RU2018144299A (en) 2020-06-17
AU2017265723B2 (en) 2023-04-13
AU2017265723A1 (en) 2018-12-20
KR102378346B1 (en) 2022-03-25
EP3460058A1 (en) 2019-03-27
CN115537454A (en) 2022-12-30

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