SG11201702074VA - High efficiency, high throughput generation of genetically modified mammals by electroporation - Google Patents

High efficiency, high throughput generation of genetically modified mammals by electroporation

Info

Publication number
SG11201702074VA
SG11201702074VA SG11201702074VA SG11201702074VA SG11201702074VA SG 11201702074V A SG11201702074V A SG 11201702074VA SG 11201702074V A SG11201702074V A SG 11201702074VA SG 11201702074V A SG11201702074V A SG 11201702074VA SG 11201702074V A SG11201702074V A SG 11201702074VA
Authority
SG
Singapore
Prior art keywords
electroporation
genetically modified
high efficiency
throughput generation
high throughput
Prior art date
Application number
SG11201702074VA
Inventor
Wenning Qin
Stephanie Lee Dion
Peter Michael Kutny
Haoyi Wang
Original Assignee
Jackson Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jackson Lab filed Critical Jackson Lab
Publication of SG11201702074VA publication Critical patent/SG11201702074VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
SG11201702074VA 2014-09-29 2015-09-29 High efficiency, high throughput generation of genetically modified mammals by electroporation SG11201702074VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462056687P 2014-09-29 2014-09-29
PCT/US2015/052928 WO2016054032A1 (en) 2014-09-29 2015-09-29 High efficiency, high throughput generation of genetically modified mammals by electroporation

Publications (1)

Publication Number Publication Date
SG11201702074VA true SG11201702074VA (en) 2017-04-27

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201902811TA SG10201902811TA (en) 2014-09-29 2015-09-29 High efficiency, high throughput generation of genetically modified mammals by electroporation
SG11201702074VA SG11201702074VA (en) 2014-09-29 2015-09-29 High efficiency, high throughput generation of genetically modified mammals by electroporation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201902811TA SG10201902811TA (en) 2014-09-29 2015-09-29 High efficiency, high throughput generation of genetically modified mammals by electroporation

Country Status (8)

Country Link
US (1) US20170298390A1 (en)
EP (1) EP3201343B1 (en)
JP (1) JP2017534295A (en)
CN (1) CN107002098A (en)
AU (1) AU2015323973A1 (en)
CA (1) CA2961954A1 (en)
SG (2) SG10201902811TA (en)
WO (1) WO2016054032A1 (en)

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US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
AU2015349692B2 (en) 2014-11-21 2021-10-28 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide RNAs
WO2016133165A1 (en) * 2015-02-19 2016-08-25 国立大学法人徳島大学 METHOD FOR TRANSFERRING Cas9 mRNA INTO MAMMALIAN FERTILIZED EGG BY ELECTROPORATION
EP3370513A1 (en) 2015-11-06 2018-09-12 The Jackson Laboratory Large genomic dna knock-in and uses thereof
EP3402327A1 (en) * 2016-01-15 2018-11-21 The Jackson Laboratory Genetically modified non-human mammals by multi-cycle electroporation of cas9 protein
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
CA3047313A1 (en) 2016-12-16 2018-06-21 B-Mogen Biotechnologies, Inc. Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods
BR112019018124A2 (en) 2017-03-22 2020-04-07 Intellia Therapeutics Inc compositions and methods for immunooncology
WO2018227025A1 (en) * 2017-06-07 2018-12-13 Arc Bio, Llc Creation and use of guide nucleic acids
JP7179306B2 (en) * 2017-10-06 2022-11-29 株式会社グリラス Method for producing genetically modified incomplete metamorphosis insects
CN107988267A (en) * 2017-12-18 2018-05-04 赛业（苏州）生物科技有限公司 A kind of high throughput carries out embryonated egg the electric shifting method of gene editing
WO2019246486A2 (en) 2018-06-21 2019-12-26 B-Mogen Biotechnologies, Inc. ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS
CN110452929B (en) * 2019-07-09 2021-07-20 中山大学 Construction method of non-chimeric gene editing pig embryo model
EP4192938A1 (en) * 2020-08-07 2023-06-14 The Jackson Laboratory Single generation targeted gene integration
CN113558009B (en) * 2021-07-07 2022-10-14 赛业（苏州）生物科技有限公司 Rescue method for chimera mouse reproductive inheritance obtained by using ES targeting technology

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US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
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JP5774657B2 (en) * 2013-10-04 2015-09-09 国立大学法人京都大学 Method for genetic modification of mammals using electroporation

Also Published As

Publication number Publication date
JP2017534295A (en) 2017-11-24
WO2016054032A1 (en) 2016-04-07
EP3201343A4 (en) 2018-04-04
EP3201343B1 (en) 2022-03-23
CN107002098A (en) 2017-08-01
AU2015323973A1 (en) 2017-04-20
EP3201343A1 (en) 2017-08-09
SG10201902811TA (en) 2019-04-29
US20170298390A1 (en) 2017-10-19
CA2961954A1 (en) 2016-04-07

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