SG11201607012TA - Methods for reducing aggregate content of protein preparations by treatment with aryl anions - Google Patents

Methods for reducing aggregate content of protein preparations by treatment with aryl anions

Info

Publication number
SG11201607012TA
SG11201607012TA SG11201607012TA SG11201607012TA SG11201607012TA SG 11201607012T A SG11201607012T A SG 11201607012TA SG 11201607012T A SG11201607012T A SG 11201607012TA SG 11201607012T A SG11201607012T A SG 11201607012TA SG 11201607012T A SG11201607012T A SG 11201607012TA
Authority
SG
Singapore
Prior art keywords
treatment
methods
protein preparations
aggregate content
reducing aggregate
Prior art date
Application number
SG11201607012TA
Inventor
Peter Stanley Gagnon
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201607012TA publication Critical patent/SG11201607012TA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201607012TA 2014-02-27 2014-02-27 Methods for reducing aggregate content of protein preparations by treatment with aryl anions SG11201607012TA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2014/000085 WO2015130221A1 (en) 2014-02-27 2014-02-27 Methods for reducing aggregate content of protein preparations by treatment with aryl anions

Publications (1)

Publication Number Publication Date
SG11201607012TA true SG11201607012TA (en) 2016-09-29

Family

ID=54009417

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201607012TA SG11201607012TA (en) 2014-02-27 2014-02-27 Methods for reducing aggregate content of protein preparations by treatment with aryl anions

Country Status (7)

Country Link
US (1) US10329322B2 (en)
EP (1) EP3116892A4 (en)
JP (1) JP6403790B2 (en)
KR (1) KR20160117628A (en)
CN (1) CN106061993A (en)
SG (1) SG11201607012TA (en)
WO (1) WO2015130221A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014133458A1 (en) * 2013-02-26 2014-09-04 Agency For Science, Technology And Research Protein purification in the presence of nonionic organic polymers and electropositive surfaces
US10253063B2 (en) * 2013-02-28 2019-04-09 Agency For Science, Technology And Research Protein purification in the presence of nonionic organic polymers at elevated conductivity

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283339A (en) * 1988-11-23 1994-02-01 California Institute Of Technology Immobilized metal aqueous two-phase extraction and precipitation
DE19544297A1 (en) * 1995-11-28 1997-06-05 Behringwerke Ag Process for the removal of aromatic compounds from product-containing solutions
CN103717284B (en) * 2011-06-08 2016-02-24 新加坡科技研究局 By retraining common hydration chromatogram purification biological products
JP6268167B2 (en) * 2012-05-31 2018-01-24 エイジェンシー フォー サイエンス，テクノロジー アンド リサーチ Method of using mixed multifunctional surfaces to reduce aggregate content in protein formulations
CN104507953B (en) 2012-05-31 2018-05-18 新加坡科技研究局 The method for handling to reduce protein in protein formulation-pollutant compound and aggregation level by using electropositive organic additive

Also Published As

Publication number Publication date
US10329322B2 (en) 2019-06-25
EP3116892A4 (en) 2017-11-22
EP3116892A1 (en) 2017-01-18
CN106061993A (en) 2016-10-26
KR20160117628A (en) 2016-10-10
WO2015130221A1 (en) 2015-09-03
JP6403790B2 (en) 2018-10-10
JP2017507940A (en) 2017-03-23
US20160362447A1 (en) 2016-12-15

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