SG11201607001PA - Methods for reducing chromatin content in protein preparations by treatment with alkyl cations - Google Patents
Methods for reducing chromatin content in protein preparations by treatment with alkyl cationsInfo
- Publication number
- SG11201607001PA SG11201607001PA SG11201607001PA SG11201607001PA SG11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA
- Authority
- SG
- Singapore
- Prior art keywords
- treatment
- methods
- protein preparations
- chromatin content
- alkyl cations
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/265—Adsorption chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/32—Extraction; Separation; Purification by precipitation as complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/SG2014/000087 WO2015130223A1 (en) | 2014-02-27 | 2014-02-27 | Methods for reducing chromatin content in protein preparations by treatment with alkyl cations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201607001PA true SG11201607001PA (en) | 2016-09-29 |
Family
ID=54009419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201607001PA SG11201607001PA (en) | 2014-02-27 | 2014-02-27 | Methods for reducing chromatin content in protein preparations by treatment with alkyl cations |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10023609B2 (en) |
| EP (1) | EP3110830A4 (en) |
| JP (1) | JP2017506646A (en) |
| KR (1) | KR20160127087A (en) |
| CN (1) | CN106061992A (en) |
| SG (1) | SG11201607001PA (en) |
| WO (1) | WO2015130223A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201610955SA (en) * | 2013-02-26 | 2017-02-27 | Agency Science Tech & Res | Protein purification in the presence of nonionic organic polymers and electropositive surfaces |
| CN105008384A (en) * | 2013-02-28 | 2015-10-28 | 新加坡科技研究局 | Protein purification in the presence of nonionic organic polymers at elevated conductivity |
| EP3442988A4 (en) | 2016-06-15 | 2020-02-26 | Agency for Science, Technology and Research | A method of enhancing the performance of chromatography methods for purification of proteins |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU597924B2 (en) * | 1985-12-11 | 1990-06-14 | Natinco Nv | Solubilization of protein aggregates |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| JP3150266B2 (en) * | 1994-04-01 | 2001-03-26 | 江崎グリコ株式会社 | Glucan having cyclic structure and method for producing the same |
| JP2003128699A (en) * | 2001-10-22 | 2003-05-08 | Ezaki Glico Co Ltd | Refolding method of antibody |
| US8759617B2 (en) * | 2010-09-21 | 2014-06-24 | National Institute Of Agrobiological Sciences | Method for extraction and purification of recombinant proteins from transgenic plants |
| US20130203969A1 (en) * | 2011-08-19 | 2013-08-08 | Emd Millipore Corporation | Use of small molecules in methods for purification of biomolecules |
| JP6297029B2 (en) | 2012-05-31 | 2018-03-20 | エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ | Chromatographic purification of immunoglobulin G preparations with particles having various functional groups |
| GB2511140A (en) | 2013-02-26 | 2014-08-27 | Shayonano Singapore Pte Ltd | Flame retardant composite particles |
| EP2961763A4 (en) * | 2013-02-28 | 2016-10-12 | Agency Science Tech & Res | Chromatographic purification of antibodies from chromatin-deficient cell culture harvests |
-
2014
- 2014-02-27 WO PCT/SG2014/000087 patent/WO2015130223A1/en active Application Filing
- 2014-02-27 CN CN201480076388.1A patent/CN106061992A/en active Pending
- 2014-02-27 US US15/121,686 patent/US10023609B2/en not_active Expired - Fee Related
- 2014-02-27 JP JP2016553510A patent/JP2017506646A/en active Pending
- 2014-02-27 KR KR1020167026576A patent/KR20160127087A/en not_active Application Discontinuation
- 2014-02-27 SG SG11201607001PA patent/SG11201607001PA/en unknown
- 2014-02-27 EP EP14883801.4A patent/EP3110830A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| US20160362448A1 (en) | 2016-12-15 |
| KR20160127087A (en) | 2016-11-02 |
| JP2017506646A (en) | 2017-03-09 |
| WO2015130223A1 (en) | 2015-09-03 |
| EP3110830A1 (en) | 2017-01-04 |
| US10023609B2 (en) | 2018-07-17 |
| EP3110830A4 (en) | 2017-10-04 |
| CN106061992A (en) | 2016-10-26 |
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