SG11201607001PA - Methods for reducing chromatin content in protein preparations by treatment with alkyl cations - Google Patents

Methods for reducing chromatin content in protein preparations by treatment with alkyl cations

Info

Publication number
SG11201607001PA
SG11201607001PA SG11201607001PA SG11201607001PA SG11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA SG 11201607001P A SG11201607001P A SG 11201607001PA
Authority
SG
Singapore
Prior art keywords
treatment
methods
protein preparations
chromatin content
alkyl cations
Prior art date
Application number
SG11201607001PA
Inventor
Peter Stanley Gagnon
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201607001PA publication Critical patent/SG11201607001PA/en

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/265Adsorption chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • C07K1/32Extraction; Separation; Purification by precipitation as complexes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32Bonded phase chromatography
    • B01D15/325Reversed phase
    • B01D15/327Reversed phase with hydrophobic interaction
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804Affinity chromatography
    • B01D15/3828Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
SG11201607001PA 2014-02-27 2014-02-27 Methods for reducing chromatin content in protein preparations by treatment with alkyl cations SG11201607001PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2014/000087 WO2015130223A1 (en) 2014-02-27 2014-02-27 Methods for reducing chromatin content in protein preparations by treatment with alkyl cations

Publications (1)

Publication Number Publication Date
SG11201607001PA true SG11201607001PA (en) 2016-09-29

Family

ID=54009419

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201607001PA SG11201607001PA (en) 2014-02-27 2014-02-27 Methods for reducing chromatin content in protein preparations by treatment with alkyl cations

Country Status (7)

Country Link
US (1) US10023609B2 (en)
EP (1) EP3110830A4 (en)
JP (1) JP2017506646A (en)
KR (1) KR20160127087A (en)
CN (1) CN106061992A (en)
SG (1) SG11201607001PA (en)
WO (1) WO2015130223A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10112971B2 (en) * 2013-02-26 2018-10-30 Agency For Science, Technology And Research Protein purification in the presence of nonionic organic polymers and electropositive surfaces
JP2016509069A (en) * 2013-02-28 2016-03-24 エイジェンシー・フォー・サイエンス,テクノロジー・アンド・リサーチ Protein purification with high conductivity in the presence of nonionic organic polymers
WO2017217930A1 (en) 2016-06-15 2017-12-21 Agency For Science, Technology And Research A method of enhancing the performance of chromatography methods for purification of proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU597924B2 (en) * 1985-12-11 1990-06-14 Natinco Nv Solubilization of protein aggregates
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
JP3150266B2 (en) * 1994-04-01 2001-03-26 江崎グリコ株式会社 Glucan having cyclic structure and method for producing the same
JP2003128699A (en) * 2001-10-22 2003-05-08 Ezaki Glico Co Ltd Refolding method of antibody
US8759617B2 (en) * 2010-09-21 2014-06-24 National Institute Of Agrobiological Sciences Method for extraction and purification of recombinant proteins from transgenic plants
CN103732253A (en) * 2011-08-19 2014-04-16 Emd密理博公司 Use of small molecules in methods for purification of biomolecules
EP2855504B1 (en) 2012-05-31 2018-10-24 Agency For Science, Technology And Research Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities
GB2511140A (en) 2013-02-26 2014-08-27 Shayonano Singapore Pte Ltd Flame retardant composite particles
SG11201505192RA (en) * 2013-02-28 2015-08-28 Agency Science Tech & Res Chromatographic purification of antibodies from chromatin-deficient cell culture harvests

Also Published As

Publication number Publication date
KR20160127087A (en) 2016-11-02
CN106061992A (en) 2016-10-26
US10023609B2 (en) 2018-07-17
EP3110830A4 (en) 2017-10-04
JP2017506646A (en) 2017-03-09
EP3110830A1 (en) 2017-01-04
US20160362448A1 (en) 2016-12-15
WO2015130223A1 (en) 2015-09-03

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