SG11201604889UA - Method for isolating separate types of micro-rna from biological fluids containing exosomes - Google Patents

Method for isolating separate types of micro-rna from biological fluids containing exosomes

Info

Publication number
SG11201604889UA
SG11201604889UA SG11201604889UA SG11201604889UA SG11201604889UA SG 11201604889U A SG11201604889U A SG 11201604889UA SG 11201604889U A SG11201604889U A SG 11201604889UA SG 11201604889U A SG11201604889U A SG 11201604889UA SG 11201604889U A SG11201604889U A SG 11201604889UA
Authority
SG
Singapore
Prior art keywords
rna
micro
biological fluids
fluids containing
separate types
Prior art date
Application number
SG11201604889UA
Inventor
Aleksandr Andreevich Abramov
Dmitrij Aleksandrovich Murashko
Vadim Igorevich Pospelov
Original Assignee
Mamontova Marina Vasilevna
Vadim Igorevich Pospelov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mamontova Marina Vasilevna, Vadim Igorevich Pospelov filed Critical Mamontova Marina Vasilevna
Publication of SG11201604889UA publication Critical patent/SG11201604889UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201604889UA 2013-12-16 2014-12-12 Method for isolating separate types of micro-rna from biological fluids containing exosomes SG11201604889UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2013155716/10A RU2548816C1 (en) 2013-12-16 2013-12-16 Method of extraction of microrna from biological fluids
PCT/RU2014/000941 WO2015094017A1 (en) 2013-12-16 2014-12-12 Method for isolating separate types of micro-rna from biological fluids containing exosomes

Publications (1)

Publication Number Publication Date
SG11201604889UA true SG11201604889UA (en) 2016-07-28

Family

ID=53289500

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201604889UA SG11201604889UA (en) 2013-12-16 2014-12-12 Method for isolating separate types of micro-rna from biological fluids containing exosomes

Country Status (3)

Country Link
RU (1) RU2548816C1 (en)
SG (1) SG11201604889UA (en)
WO (1) WO2015094017A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2710368C2 (en) * 2018-05-29 2019-12-26 Станислав Евгеньевич Волчков METHOD OF PRODUCING AND CONCENTRATING microRNA-CONTAINING EXOSOMES OF MULTIPOTENT MESENCHYMAL STROMAL CELLS FOR USE IN COSMETIC AND MEDICINAL AGENTS FOR STIMULATING REGENERATIVE PROCESSES AND SLOWING DOWN AGING PROCESSES
RU2729423C2 (en) * 2018-12-19 2020-08-06 федеральное государственное автономное образовательное учреждение высшего образования "Российский университет дружбы народов" (РУДН) Method of extracting free and exosomal micro-rna from saliva
RU2748767C1 (en) * 2020-08-27 2021-05-31 Гордейчук Владимир Евгеньевич Method for loading exosomes with small non-coding rnas

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9307501A (en) * 2000-09-22 2002-04-02 Virxsys Improved conditionally replicating vectors, methods for their production and use
US9078914B2 (en) * 2009-09-10 2015-07-14 Velin-Pharma A/S Method for the preparation of micro-RNA and its therapeutic application
US8530228B2 (en) * 2010-12-28 2013-09-10 Bexmart Integrated versatile and systems preparation of specimens

Also Published As

Publication number Publication date
WO2015094017A1 (en) 2015-06-25
RU2548816C1 (en) 2015-04-20

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