SG11201602427UA - Method and apparatus for isolation, capture and molecular analysis of target particles - Google Patents

Method and apparatus for isolation, capture and molecular analysis of target particles

Info

Publication number
SG11201602427UA
SG11201602427UA SG11201602427UA SG11201602427UA SG11201602427UA SG 11201602427U A SG11201602427U A SG 11201602427UA SG 11201602427U A SG11201602427U A SG 11201602427UA SG 11201602427U A SG11201602427U A SG 11201602427UA SG 11201602427U A SG11201602427U A SG 11201602427UA
Authority
SG
Singapore
Prior art keywords
capture
isolation
target particles
molecular analysis
molecular
Prior art date
Application number
SG11201602427UA
Inventor
Anoop Menachery
Vishal Gupta
David K Hasegawa
Ronald Pethig
Original Assignee
Apocell Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Apocell Inc filed Critical Apocell Inc
Publication of SG11201602427UA publication Critical patent/SG11201602427UA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/004—Field flow fractionation characterised by opposing force
    • G01N2030/0065—Dielectric FFF, i.e. opposing forces dominate hydrodynamic lift forces and steric effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Hydrology & Water Resources (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11201602427UA 2013-08-29 2014-08-28 Method and apparatus for isolation, capture and molecular analysis of target particles SG11201602427UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361871624P 2013-08-29 2013-08-29
PCT/US2014/053107 WO2015031586A1 (en) 2013-08-29 2014-08-28 Method and apparatus for isolation, capture and molecular analysis of target particles

Publications (1)

Publication Number Publication Date
SG11201602427UA true SG11201602427UA (en) 2016-05-30

Family

ID=52587323

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201602427UA SG11201602427UA (en) 2013-08-29 2014-08-28 Method and apparatus for isolation, capture and molecular analysis of target particles

Country Status (6)

Country Link
US (1) US20160209299A1 (en)
EP (1) EP3039416B1 (en)
KR (1) KR20160048929A (en)
CN (1) CN105683745A (en)
SG (1) SG11201602427UA (en)
WO (1) WO2015031586A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6639906B2 (en) * 2015-12-25 2020-02-05 東ソー株式会社 Biological sample detection method
JP2018021895A (en) * 2016-07-22 2018-02-08 東ソー株式会社 Method of detecting and recovering undifferentiated cell
US20180093271A1 (en) * 2016-09-30 2018-04-05 The University Of Tokyo Microdevice for Capturing Particles, and Method for Capturing, Concentrating, or Separating Particles Using the Same
JP7085190B2 (en) * 2018-03-30 2022-06-16 兵庫県公立大学法人 Quantitative method of target substance using aptamer
DE102018210665A1 (en) 2018-06-29 2020-01-02 Robert Bosch Gmbh Microfluidic flow cell and method for separating cells
DE102018210696A1 (en) 2018-06-29 2020-01-02 Robert Bosch Gmbh Device and method for the dielectrophoretic separation of target particles in a liquid
DE102018211281A1 (en) 2018-07-09 2020-01-09 Robert Bosch Gmbh Microfluidic device and method for diluting and separating particles of a sample
KR102102534B1 (en) * 2018-07-11 2020-04-23 주식회사 엑스와이지플랫폼 Bio-Sensor Having Interdigitated Micro-Electrode using Dielectric Substance Electrophoresis, and Method for Detecting Bio-Material Using The Same
CN108982630B (en) * 2018-07-20 2020-02-14 山东理工大学 Preparation method and application of sandwich type electrochemical immunosensor for detecting prostate specific antigen
CN109596730B (en) * 2018-12-14 2021-11-23 沈阳药科大学 Method for simultaneously determining hormone and antibiotic in fat food sample
CN112034029B (en) * 2020-09-11 2022-12-20 华南师范大学 Microfluid dielectrophoresis separation device and manufacturing method thereof
CN114839291B (en) * 2022-04-28 2023-05-12 山东大学 Detection method of various tyrosine kinase inhibitor drugs
CN118139976A (en) * 2022-05-24 2024-06-04 拜奥雅德科技有限公司 High throughput selection and manufacture of biomaterial-encapsulated cell clusters and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
GB9306729D0 (en) * 1993-03-31 1993-05-26 British Tech Group Improvements in separators
GB9916850D0 (en) * 1999-07-20 1999-09-22 Univ Wales Bangor Dielectrophoretic apparatus & method
CN100494360C (en) * 2001-03-22 2009-06-03 博奥生物有限公司 Process for separating cells and its application
EP1583956A4 (en) * 2002-12-20 2009-07-15 Univ Texas Methods and apparatus for electrosmear analysis
ITBO20040420A1 (en) * 2004-07-07 2004-10-07 Type S R L METAL CUTTING AND FORMING MACHINE
US7551419B2 (en) * 2006-06-05 2009-06-23 Sri International Electroadhesion
KR20100085911A (en) * 2007-10-09 2010-07-29 유니버시티 오브 노트르 담 디락 Microfluidic platforms for multi-target detection
US20120031759A1 (en) * 2009-01-30 2012-02-09 Natural And Medical Sciences Institute At The University Of Tubingen Dielectrophoretic device with actuator
DE102009028493B4 (en) * 2009-08-13 2023-08-24 Robert Bosch Gmbh microfluidic cell
FR2964243A1 (en) * 2010-08-27 2012-03-02 Commissariat Energie Atomique INTERMITTENT CONTACT DEVICE IMPROVED BY DIELECTROPHORESIS
WO2013028573A1 (en) * 2011-08-19 2013-02-28 Allan Yang Wu Gradient array dielectrophoresis separation (grads) with concomitant light therapy
US8941974B2 (en) * 2011-09-09 2015-01-27 Xilinx, Inc. Interdigitated capacitor having digits of varying width

Also Published As

Publication number Publication date
EP3039416A1 (en) 2016-07-06
CN105683745A (en) 2016-06-15
KR20160048929A (en) 2016-05-04
WO2015031586A1 (en) 2015-03-05
EP3039416B1 (en) 2022-05-04
EP3039416A4 (en) 2017-04-26
US20160209299A1 (en) 2016-07-21

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