SG11201408736SA - Massively parallel combinatorial genetics - Google Patents
Massively parallel combinatorial geneticsInfo
- Publication number
- SG11201408736SA SG11201408736SA SG11201408736SA SG11201408736SA SG11201408736SA SG 11201408736S A SG11201408736S A SG 11201408736SA SG 11201408736S A SG11201408736S A SG 11201408736SA SG 11201408736S A SG11201408736S A SG 11201408736SA SG 11201408736S A SG11201408736S A SG 11201408736SA
- Authority
- SG
- Singapore
- Prior art keywords
- massively parallel
- parallel combinatorial
- combinatorial genetics
- genetics
- massively
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00742—Type of codes
- G01N2035/00752—Type of codes bar codes
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261666081P | 2012-06-29 | 2012-06-29 | |
| PCT/US2013/048619 WO2014005042A2 (en) | 2012-06-29 | 2013-06-28 | Massively parallel combinatorial genetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201408736SA true SG11201408736SA (en) | 2015-03-30 |
Family
ID=48747811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201408736SA SG11201408736SA (en) | 2012-06-29 | 2013-06-28 | Massively parallel combinatorial genetics |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9315806B2 (en) |
| EP (1) | EP2867361B1 (en) |
| JP (1) | JP6401700B2 (en) |
| CN (1) | CN104508130B (en) |
| AU (1) | AU2013282323B2 (en) |
| CA (1) | CA2877962C (en) |
| DK (1) | DK2867361T3 (en) |
| ES (1) | ES2663424T3 (en) |
| HK (2) | HK1207886A1 (en) |
| NO (1) | NO2897610T3 (en) |
| PL (1) | PL2867361T3 (en) |
| PT (1) | PT2867361T (en) |
| SG (1) | SG11201408736SA (en) |
| WO (1) | WO2014005042A2 (en) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| JP6232074B2 (en) * | 2012-12-19 | 2017-11-15 | ヘルゲ・ジーラー | Compositions and methods for producing modified and improved cells and organisms |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| EP3066201B1 (en) | 2013-11-07 | 2018-03-07 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015288389B2 (en) * | 2014-07-07 | 2021-05-13 | Astrego Diagnostics Ab | Phenotypic characterization and in situ genotyping of a library of genetically different cells |
| WO2016011080A2 (en) | 2014-07-14 | 2016-01-21 | The Regents Of The University Of California | Crispr/cas transcriptional modulation |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016070037A2 (en) * | 2014-10-31 | 2016-05-06 | Massachusetts Institute Of Technology | Massively parallel combinatorial genetics for crispr |
| WO2016115033A1 (en) * | 2015-01-12 | 2016-07-21 | Massachusetts Institute Of Technology | Microrna combinations for anti-cancer therapeutics |
| US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| EP3448996A1 (en) * | 2016-04-26 | 2019-03-06 | Massachusetts Institute of Technology | Extensible recombinase cascades |
| EP4219731A3 (en) * | 2016-05-18 | 2023-08-09 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nucleobase editors and uses thereof |
| CN109804066A (en) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | Programmable CAS9- recombination enzyme fusion proteins and application thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| CN107784198B (en) * | 2016-08-26 | 2021-06-15 | 深圳华大基因科技服务有限公司 | Combined assembly method and system for second-generation sequence and third-generation single-molecule real-time sequencing sequence |
| AU2017342543A1 (en) | 2016-10-14 | 2019-05-02 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018081113A1 (en) | 2016-10-24 | 2018-05-03 | Sawaya Sterling | Concealing information present within nucleic acids |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| JP7191388B2 (en) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11293018B2 (en) | 2017-06-09 | 2022-04-05 | The Board Of Trustees Of The Leland Stanford Junior University | Manipulation of nuclear architecture |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| EP3697906A1 (en) | 2017-10-16 | 2020-08-26 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3853363A4 (en) | 2018-09-19 | 2022-12-14 | The University of Hong Kong | Improved high-throughput combinatorial genetic modification system and optimized cas9 enzyme variants |
| CN109801679B (en) * | 2019-01-15 | 2021-02-02 | 广州柿宝生物科技有限公司 | Mathematical sequence reconstruction method for long-chain molecules |
| DE112020001342T5 (en) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Methods and compositions for editing nucleotide sequences |
| JP2023525304A (en) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| WO2023022659A1 (en) | 2021-08-19 | 2023-02-23 | Engine Biosciences Pte. Ltd. | Compositions and methods for generating synthetic lethality in tumors |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US211103A (en) | 1879-01-07 | Improvement in nut-locks | ||
| DE60141533D1 (en) * | 2000-12-08 | 2010-04-22 | Life Technologies Corp | UKLEINE ACID MOLECULAR USING MULTIPLE DETECTION CENTERS |
| CA2536565A1 (en) | 2003-09-10 | 2005-05-12 | Althea Technologies, Inc. | Expression profiling using microarrays |
| US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| US8221982B2 (en) * | 2008-11-19 | 2012-07-17 | Amyris, Inc. | Compositions and methods for the assembly of polynucleotides |
| US9255291B2 (en) | 2010-05-06 | 2016-02-09 | Bioo Scientific Corporation | Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing |
| WO2012019765A1 (en) | 2010-08-10 | 2012-02-16 | European Molecular Biology Laboratory (Embl) | Methods and systems for tracking samples and sample combinations |
-
2013
- 2013-06-28 AU AU2013282323A patent/AU2013282323B2/en active Active
- 2013-06-28 JP JP2015520583A patent/JP6401700B2/en active Active
- 2013-06-28 SG SG11201408736SA patent/SG11201408736SA/en unknown
- 2013-06-28 EP EP13734638.3A patent/EP2867361B1/en active Active
- 2013-06-28 US US14/411,628 patent/US9315806B2/en active Active
- 2013-06-28 CN CN201380040777.4A patent/CN104508130B/en active Active
- 2013-06-28 ES ES13734638.3T patent/ES2663424T3/en active Active
- 2013-06-28 PT PT137346383T patent/PT2867361T/en unknown
- 2013-06-28 DK DK13734638.3T patent/DK2867361T3/en active
- 2013-06-28 PL PL13734638T patent/PL2867361T3/en unknown
- 2013-06-28 WO PCT/US2013/048619 patent/WO2014005042A2/en active Application Filing
- 2013-06-28 CA CA2877962A patent/CA2877962C/en active Active
- 2013-09-19 NO NO13839158A patent/NO2897610T3/no unknown
-
2015
- 2015-09-01 HK HK15108525.0A patent/HK1207886A1/en unknown
- 2015-11-06 HK HK15110947.6A patent/HK1210217A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN104508130A (en) | 2015-04-08 |
| DK2867361T3 (en) | 2018-01-29 |
| EP2867361B1 (en) | 2017-11-01 |
| US9315806B2 (en) | 2016-04-19 |
| US20150141263A1 (en) | 2015-05-21 |
| WO2014005042A2 (en) | 2014-01-03 |
| AU2013282323A1 (en) | 2015-02-19 |
| ES2663424T3 (en) | 2018-04-12 |
| NO2897610T3 (en) | 2018-06-09 |
| CA2877962C (en) | 2018-06-05 |
| CN104508130B (en) | 2016-09-28 |
| PL2867361T3 (en) | 2018-07-31 |
| PT2867361T (en) | 2018-02-07 |
| HK1207886A1 (en) | 2016-02-12 |
| WO2014005042A3 (en) | 2014-02-27 |
| JP6401700B2 (en) | 2018-10-10 |
| AU2013282323B2 (en) | 2018-05-10 |
| HK1210217A1 (en) | 2016-04-15 |
| JP2015521850A (en) | 2015-08-03 |
| EP2867361A2 (en) | 2015-05-06 |
| CA2877962A1 (en) | 2014-01-03 |
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