SG11201407879SA - Biosynthetic pathways, recombinant cells, and methods - Google Patents

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180810 A1 (51) International Patent Classification: C12P 7/62 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/031470 14 March 2013 (14.03.2013) English English (30) Priority Data: 61/652,505 29 May 2012 (29.05.2012) US (71) Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA [US/US]; Office For Technology Commer­ cialization, 1000 Westgate Drive, Suite 160, Saint Paul, MN 55114-8658 (US). (72) Inventors; and (71) Applicants ZHANG, Kechun : [CN/US]; 1051 5th Street Se, Minneapolis, MN 55414 (US). XIONG, Mingyong [CN/US]; 1194 Fifield Avenue, St. Paul, MN 55108 (US). TAI, Yi-shu [—/US]; 1920 S 1st Steet, Apt. 2108, Min­ neapolis, MN 55454 (US). (74) Agent: GRAM, Christopher, D.; Mueting, Raasch & Gebhardt, P.A., P.O. Box 581336, Minneapolis, MN 55458-1336 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: BIOSYNTHETIC PATHWAYS, RECOMBINANT CELLS, AND METHODS Figure 2 j youlysis Glucose^ . - - . . o!oS i!vC II-JU < © i-H 00 o 00 ' COOH Pyil.VMlfi Pathway I ] .... \" BuDH 1 p.rtA •COOH Ketovaline I KIVD Ur.hutyrir. ar.irl ! iadDX H.tthw.iy II .sobutyry-CoA Isnhi.tyra Oi-jhydf: J, >'f' iD j' OH isob'jtarcl luxE Isobutyl-isobutyrate i-H o CJ (57) Abstract: This disclosure describes engineered biosynthetic pathways, recombinant cells, and methods relating to biosynthesis of esters. The recombinant cells may be modified to exhibit increased biosynthesis of an ester compared to a wild-type control. The recombinant cell may be incubated in medium that includes carbon source under conditions a effective for the recombinant cell to produce an ester. This disclosure also describes a method that generally includes introducing into a host cell heterologous a polynuc - leotide encoding at least one polypeptide that catalyzes a step in converting a carbon source to an ester, wherein the at least one polynucleotide operably linked is to a promoter so that the modified host cell catalyzes conversion of the carbon source an ester. to