SG10201908494XA - Method and device for acquiring and displaying an immunofluorescence image of a biological sample - Google Patents

Method and device for acquiring and displaying an immunofluorescence image of a biological sample

Info

Publication number
SG10201908494XA
SG10201908494XA SG10201908494XA SG10201908494XA SG10201908494XA SG 10201908494X A SG10201908494X A SG 10201908494XA SG 10201908494X A SG10201908494X A SG 10201908494XA SG 10201908494X A SG10201908494X A SG 10201908494XA SG 10201908494X A SG10201908494X A SG 10201908494XA
Authority
SG
Singapore
Prior art keywords
acquiring
displaying
biological sample
immunofluorescence image
immunofluorescence
Prior art date
Application number
SG10201908494XA
Other languages
English (en)
Inventor
Tilman Johannes Sumpf
Michael Pape
Martin Hagen-Eggert
Christian Piepke
Thomas Laudan
Michael Falkert
Markus Morrin
Kristin Rentzsch
Original Assignee
Euroimmun Medizinische Labordiagnostika Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Euroimmun Medizinische Labordiagnostika Ag filed Critical Euroimmun Medizinische Labordiagnostika Ag
Publication of SG10201908494XA publication Critical patent/SG10201908494XA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/60—Control of cameras or camera modules
    • H04N23/667—Camera operation mode switching, e.g. between still and video, sport and normal or high- and low-resolution modes
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/26—Stages; Adjusting means therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/60—Control of cameras or camera modules
    • H04N23/61—Control of cameras or camera modules based on recognised objects
    • H04N23/611—Control of cameras or camera modules based on recognised objects where the recognised objects include parts of the human body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B6/00—Apparatus or devices for radiation diagnosis; Apparatus or devices for radiation diagnosis combined with radiation therapy equipment
    • A61B6/48—Diagnostic techniques
    • A61B6/485—Diagnostic techniques involving fluorescence X-ray imaging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B6/00—Apparatus or devices for radiation diagnosis; Apparatus or devices for radiation diagnosis combined with radiation therapy equipment
    • A61B6/48—Diagnostic techniques
    • A61B6/486—Diagnostic techniques involving generating temporal series of image data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts
    • G01N2021/0112—Apparatus in one mechanical, optical or electronic block
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Multimedia (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Signal Processing (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Biophysics (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
SG10201908494XA 2018-09-13 2019-09-13 Method and device for acquiring and displaying an immunofluorescence image of a biological sample SG10201908494XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18194332.5A EP3623798B1 (de) 2018-09-13 2018-09-13 Verfahren und vorrichtung zum erfassen und darstellen eines immunfluoreszenzbildes einer biologischen probe

Publications (1)

Publication Number Publication Date
SG10201908494XA true SG10201908494XA (en) 2020-04-29

Family

ID=63579228

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201908494XA SG10201908494XA (en) 2018-09-13 2019-09-13 Method and device for acquiring and displaying an immunofluorescence image of a biological sample

Country Status (7)

Country Link
US (1) US10855923B2 (de)
EP (1) EP3623798B1 (de)
CN (1) CN110895243B (de)
AU (1) AU2019229421B2 (de)
ES (1) ES2903440T3 (de)
PL (1) PL3623798T3 (de)
SG (1) SG10201908494XA (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111795959A (zh) * 2020-07-24 2020-10-20 芯动森林(重庆)医疗科技有限公司 胶体金与荧光二合一数据采集装置和方法
CN113109307A (zh) * 2021-04-01 2021-07-13 南京超维景生物科技有限公司 位移控制方法及装置、电子设备及多光子荧光成像设备

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08223563A (ja) * 1995-02-15 1996-08-30 Meitec Corp 観察装置の試料台制御方式
DE10362120C5 (de) * 2003-02-03 2010-01-28 Carl Zeiss Surgical Gmbh Mikroskopieverfahren und Mikroskopiesystem
JP2005331889A (ja) * 2004-05-21 2005-12-02 Keyence Corp 蛍光顕微鏡及び蛍光観察方法
US20060178833A1 (en) * 2005-02-04 2006-08-10 Bauer Kenneth D System for and method of providing diagnostic information through microscopic imaging
CN2814401Y (zh) * 2005-07-27 2006-09-06 北京工业大学 生物芯片使用的毫米级微型激光诱导荧光检测仪
DE102006027518A1 (de) 2006-06-09 2007-12-13 Euroimmun Medizinische Labordiagnostika Ag Konstante Lichtquelle für die Fluoreszenzmikroskopie
US7749775B2 (en) * 2006-10-03 2010-07-06 Jonathan Scott Maher Immunoassay test device and method of use
CA2688433A1 (en) * 2007-06-06 2008-12-11 Domantis Limited Methods for selecting protease resistant polypeptides
US8264768B2 (en) * 2007-06-07 2012-09-11 Olympus Corporation Microscope system
CA2604317C (en) * 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
DE102008059328A1 (de) * 2008-11-27 2010-06-02 Carl Zeiss Microimaging Gmbh Auflösungsgesteigerte Mikroskopie
WO2011125985A1 (ja) * 2010-04-05 2011-10-13 オリンパス株式会社 顕微鏡コントローラ及び該顕微鏡コントローラを有する顕微鏡システム
DE102010016382B4 (de) * 2010-04-09 2022-06-02 Leica Microsystems Cms Gmbh Fluoreszenzmikroskop und Verfahren zur Durchführung von Multipositionierungen in einer Screening-Applikation
US20120071338A1 (en) * 2010-07-15 2012-03-22 Christopher Bowman Polymerization-based amplification for immunostaining and biodetection
DE102010035104A1 (de) 2010-08-23 2012-04-05 Euroimmun Medizinische Labordiagnostika Ag Vorrichtung und Verfahren zur automatischen Fokussierung für die Mikroskopie schwach leuchtender Substrate
JP2012168085A (ja) * 2011-02-16 2012-09-06 Ncd:Kk 特定生物成分検出方法
WO2012128305A1 (ja) * 2011-03-23 2012-09-27 ナノフォトン株式会社 顕微鏡
DE102013216124A1 (de) * 2013-08-14 2015-02-19 Carl Zeiss Microscopy Gmbh Hochauflösende 3D-Fluoreszenzmikroskopie
US9322051B2 (en) * 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
CN103767686B (zh) * 2014-01-20 2015-05-20 西安电子科技大学 一种小动物生物发光成像光源定位方法
NL2012218C2 (en) * 2014-02-06 2015-08-10 Univ Delft Tech Method and apparatus for determining a density of fluorescent markers in a sample.
JP6375925B2 (ja) * 2014-12-15 2018-08-22 コニカミノルタ株式会社 画像処理装置、画像処理システム、画像処理プログラム及び画像処理方法
DE102015121920A1 (de) * 2015-12-16 2017-06-22 Carl Zeiss Microscopy Gmbh Hochauflösendes Kurzzeit-Mikroskopieverfahren und hochauflösendes Kurzzeit-Mikroskop
CN106124616A (zh) * 2016-06-12 2016-11-16 肖小玉 一种新型微生物肥料检测装置
CN106905964B (zh) * 2017-03-29 2019-04-16 江苏师范大学 一种钛铌酸绿色上转换发光材料、制备方法及应用
EP3418789A1 (de) 2017-06-20 2018-12-26 Euroimmun Medizinische Labordiagnostika AG Verfahren und mikroskopiesystem zum aufnehmen eines bildes
GB201712032D0 (en) * 2017-07-26 2017-09-06 Bioinvent Int Ab Antibodies and uses thereof
EP3671309B1 (de) * 2018-12-20 2021-11-10 Euroimmun Medizinische Labordiagnostika AG Verfahren und mikroskopiersystem zum aufnehmen eines mikroskopischen fluoreszenzbildes eines probenbereiches mit einer biologischen probe

Also Published As

Publication number Publication date
AU2019229421A1 (en) 2020-04-02
BR102019018897A2 (pt) 2020-03-24
CN110895243A (zh) 2020-03-20
AU2019229421B2 (en) 2021-04-01
PL3623798T3 (pl) 2022-03-28
US10855923B2 (en) 2020-12-01
ES2903440T3 (es) 2022-04-01
EP3623798B1 (de) 2021-11-03
CN110895243B (zh) 2023-05-26
EP3623798A1 (de) 2020-03-18
US20200092478A1 (en) 2020-03-19

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