SG10201906501YA - 21-hydroxylation of steroids - Google Patents

21-hydroxylation of steroids

Info

Publication number
SG10201906501YA
SG10201906501YA SG10201906501YA SG10201906501YA SG10201906501YA SG 10201906501Y A SG10201906501Y A SG 10201906501YA SG 10201906501Y A SG10201906501Y A SG 10201906501YA SG 10201906501Y A SG10201906501Y A SG 10201906501YA SG 10201906501Y A SG10201906501Y A SG 10201906501YA
Authority
SG
Singapore
Prior art keywords
hydroxylation
steroids
steroid
relates
cells
Prior art date
Application number
SG10201906501YA
Inventor
Claus Lattemann
Thomas Stillger
Bernd Janocha
Hans-Falk Rasser
Sebastian Rissom
Simone Anderko
Rita Bernhardt
Frank Hannemann
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of SG10201906501YA publication Critical patent/SG10201906501YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0087—Steroid 21-monooxygenase (1.14.99.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/9901—Steroid 21-monooxygenase (1.14.99.10), i.e. steroid cytochrome P450 21-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
    • C12Y118/01002—Ferredoxin-NADP+ reductase (1.18.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
    • C12Y118/01006—Adrenodoxin-NADP+ reductase (1.18.1.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)

Abstract

21-Hydroxylation of steroids : 5 Generally, the present invention relates to the field of steroid hydroxylation. More specifically, the present invention relates to a method for the 21-hydroxylation of steroids in cells. It also relates to cells expressing a steroid 21-hydroxylating enzyme or steroid 21-hydroxylase, expression vectors comprising a nucleic acid encoding for a steroid 21-hydroxylase and a kit for carrying out the method for the 21-hydroxylation of steroids in cells. 10 [Figure no. 2]
SG10201906501YA 2014-10-30 2015-10-29 21-hydroxylation of steroids SG10201906501YA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP14306740.3A EP3015550A1 (en) 2014-10-30 2014-10-30 21-Hydroxylation of steroids

Publications (1)

Publication Number Publication Date
SG10201906501YA true SG10201906501YA (en) 2019-09-27

Family

ID=51932297

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201906501YA SG10201906501YA (en) 2014-10-30 2015-10-29 21-hydroxylation of steroids
SG11201703444SA SG11201703444SA (en) 2014-10-30 2015-10-29 21-hydroxylation of steroids

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201703444SA SG11201703444SA (en) 2014-10-30 2015-10-29 21-hydroxylation of steroids

Country Status (13)

Country Link
US (3) US10385376B2 (en)
EP (3) EP3015550A1 (en)
JP (3) JP6856528B2 (en)
CN (2) CN108138215B (en)
CA (1) CA2966067C (en)
CY (1) CY1125282T1 (en)
DK (1) DK3212800T3 (en)
ES (1) ES2898465T3 (en)
HU (1) HUE056495T2 (en)
IL (3) IL251978B (en)
SG (2) SG10201906501YA (en)
TW (2) TWI816043B (en)
WO (1) WO2016066738A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110229838B (en) * 2019-05-28 2021-01-08 浙江理工大学 Method for obtaining hydroxylated compound by biotransformation of steroid compound
CN114410727B (en) * 2022-01-25 2023-09-19 山东诺明康药物研究院有限公司 Preparation method of clavulanone

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU577965B2 (en) 1985-10-29 1988-10-06 Hooi Heng Lim Nappy change bag
GB9615032D0 (en) * 1996-07-17 1996-09-04 Univ Dundee Enzyme system
JP3344618B2 (en) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 Chaperone expression plasmid
JP4249832B2 (en) 1998-12-28 2009-04-08 タカラバイオ株式会社 Trigger factor expression plasmid
KR20050028279A (en) * 2003-09-18 2005-03-22 안태호 Increase of protein expression and catalytic activity of recombinant cytochrome p450 enzymes by co-expression with genes encoding heat shock proteins

Also Published As

Publication number Publication date
TWI701336B (en) 2020-08-11
CA2966067C (en) 2024-01-02
CN108138215A (en) 2018-06-08
TW202041676A (en) 2020-11-16
JP2024001138A (en) 2024-01-09
IL275237A (en) 2020-07-30
JP7364611B2 (en) 2023-10-18
US11608514B2 (en) 2023-03-21
IL275237B (en) 2021-09-30
JP2021097709A (en) 2021-07-01
IL251978B (en) 2020-06-30
CN108138215B (en) 2022-01-25
US11965199B2 (en) 2024-04-23
EP3212800A1 (en) 2017-09-06
HUE056495T2 (en) 2022-02-28
US10385376B2 (en) 2019-08-20
CY1125282T1 (en) 2023-03-24
TW202300653A (en) 2023-01-01
TW201625795A (en) 2016-07-16
EP3957739A1 (en) 2022-02-23
US20170321241A1 (en) 2017-11-09
US20190352690A1 (en) 2019-11-21
CA2966067A1 (en) 2016-05-06
IL285700A (en) 2021-09-30
IL251978A0 (en) 2017-06-29
CN114540453A (en) 2022-05-27
DK3212800T3 (en) 2021-12-06
WO2016066738A1 (en) 2016-05-06
JP6856528B2 (en) 2021-04-07
JP2017532057A (en) 2017-11-02
US20220228189A1 (en) 2022-07-21
EP3212800B1 (en) 2021-09-15
TWI816043B (en) 2023-09-21
EP3015550A1 (en) 2016-05-04
SG11201703444SA (en) 2017-05-30
IL285700B1 (en) 2023-01-01
IL285700B2 (en) 2023-05-01
ES2898465T3 (en) 2022-03-07

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