SG10201707153UA - Serum-free cell culture medium - Google Patents

Serum-free cell culture medium

Info

Publication number
SG10201707153UA
SG10201707153UA SG10201707153UA SG10201707153UA SG10201707153UA SG 10201707153U A SG10201707153U A SG 10201707153UA SG 10201707153U A SG10201707153U A SG 10201707153UA SG 10201707153U A SG10201707153U A SG 10201707153UA SG 10201707153U A SG10201707153U A SG 10201707153UA
Authority
SG
Singapore
Prior art keywords
serum
culture medium
cell culture
free cell
free
Prior art date
Application number
SG10201707153UA
Inventor
Shadia Oshodi
Amy Johnson
Shawn Lawrence
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51528781&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201707153U(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of SG10201707153UA publication Critical patent/SG10201707153UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0031Serum-free culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0037Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0043Medium free of human- or animal-derived components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/16Magnesium; Mg chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/34Sugars
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/36Lipids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/40Nucleotides, nucleosides or bases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/46Amines, e.g. putrescine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/04Immortalised cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00Cells for large scale production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SG10201707153UA 2013-03-15 2014-03-14 Serum-free cell culture medium SG10201707153UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361790136P 2013-03-15 2013-03-15
US14/211,245 US20140273095A1 (en) 2013-03-15 2014-03-14 Serum-Free Cell Culture Medium

Publications (1)

Publication Number Publication Date
SG10201707153UA true SG10201707153UA (en) 2017-10-30

Family

ID=51528781

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10202107444XA SG10202107444XA (en) 2013-03-15 2014-03-14 Serum-free cell culture medium
SG10201707153UA SG10201707153UA (en) 2013-03-15 2014-03-14 Serum-free cell culture medium
SG11201507072TA SG11201507072TA (en) 2013-03-15 2014-03-14 Serum-free cell culture medium

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10202107444XA SG10202107444XA (en) 2013-03-15 2014-03-14 Serum-free cell culture medium

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201507072TA SG11201507072TA (en) 2013-03-15 2014-03-14 Serum-free cell culture medium

Country Status (22)

Country Link
US (5) US20140273095A1 (en)
EP (4) EP3378932A1 (en)
JP (5) JP6781039B2 (en)
KR (6) KR102229380B1 (en)
CN (2) CN113481148A (en)
AR (1) AR095196A1 (en)
AU (4) AU2014227804B2 (en)
BR (2) BR122020021864B1 (en)
CA (5) CA2906768C (en)
DK (1) DK2970876T3 (en)
EA (3) EA202190326A3 (en)
ES (1) ES2682929T3 (en)
HK (1) HK1213289A1 (en)
HU (1) HUE040233T2 (en)
IL (5) IL281515B2 (en)
MX (3) MX357977B (en)
MY (1) MY175957A (en)
PL (1) PL2970876T3 (en)
PT (1) PT2970876T (en)
SG (3) SG10202107444XA (en)
TW (4) TWI701333B (en)
WO (1) WO2014144198A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR095196A1 (en) * 2013-03-15 2015-09-30 Regeneron Pharma SERUM FREE CELL CULTIVATION MEDIA
WO2015148515A1 (en) * 2014-03-24 2015-10-01 Biogen Ma Inc. Methods for overcoming glutamine deprivation during mammalian cell culture
TW202340452A (en) * 2015-08-04 2023-10-16 美商再生元醫藥公司 Taurine supplemented cell culture medium and methods of use
KR101936049B1 (en) * 2015-10-15 2019-01-08 (주)알테오젠 Method for manufacturing fusion proteins with IgG Fc domain
US20180355304A1 (en) * 2015-12-03 2018-12-13 Merck Patent Gmbh Chemically defined media for the growth or detection of microorganisms
KR20180047404A (en) * 2016-10-31 2018-05-10 삼성바이오에피스 주식회사 Process for reducing undesirable by-product in cell culture
CN108220228A (en) * 2016-12-09 2018-06-29 上海迈泰君奥生物技术有限公司 The method of serum-free cell culture medium and highly effective expressing recombinant protein matter
CN106635953B (en) * 2016-12-13 2021-02-19 昆明润什生物科技有限公司 Serum-free and protein-free cell culture medium
CA3066918A1 (en) 2017-06-12 2018-12-20 Bluefin Biomedicine, Inc. Anti-il1rap antibodies and antibody drug conjugates
SG11201912548XA (en) * 2017-07-06 2020-01-30 Regeneron Pharma Cell culture process for making a glycoprotein
CN108103003B (en) * 2017-12-12 2021-03-05 四川百诺吉科技有限公司 Serum-free medium adapting to PK-15 full-suspension growth, preparation method thereof and full-suspension domestication method applied to PK-15 cells
WO2019135238A1 (en) * 2018-01-03 2019-07-11 Technion Research & Development Foundation Limited Naive pluripotent stem cell media
US10961500B1 (en) * 2019-04-23 2021-03-30 Regeneron Pharmaceuticals, Inc. Cell culture medium for eukaryotic cells
US20220204920A1 (en) * 2019-05-16 2022-06-30 Formycon Ag Method for reducing methionine oxidation in recombinant proteins
CN110438066B (en) * 2019-08-19 2021-01-12 杭州百凌生物科技有限公司 Mammalian cell line 293C18P capable of being stably passaged and capable of being cultured in suspension, and preparation method and application thereof
WO2021112927A1 (en) 2019-12-06 2021-06-10 Regeneron Pharmaceuticals, Inc. Anti-vegf protein compositions and methods for producing the same
CA3165060C (en) 2020-01-21 2023-06-20 Yiming Zhao Deglycosylation methods for electrophoresis of glycosylated proteins
WO2021226444A2 (en) 2020-05-08 2021-11-11 Regeneron Pharmaceuticals, Inc. Vegf traps and mini-traps and methods for treating ocular disorders and cancer
KR20230058094A (en) 2020-08-31 2023-05-02 리제너론 파아마슈티컬스, 인크. Asparagine supply strategies to improve cell culture performance and mitigate asparagine sequence variants
EP4281542A1 (en) * 2021-01-20 2023-11-29 Regeneron Pharmaceuticals, Inc. Methods of improving protein titer in cell culture
KR20220127465A (en) 2021-03-11 2022-09-20 주식회사 만도 Steering control apparatus and method

Family Cites Families (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072565A (en) 1974-11-04 1978-02-07 The Dow Chemical Company Production of viruses in tissue culture without use of serum
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
EP0082974B1 (en) 1981-12-24 1986-05-14 Asahi Kasei Kogyo Kabushiki Kaisha Method for the cultivation of normal diploid cells and cultivation medium used therefor
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
FR2543158B1 (en) 1983-03-24 1985-11-15 Inst Nat Sante Rech Med MEDIUM FOR CULTURING ANIMAL CELLS WITHOUT SERUM, WITHOUT HORMONES AND WITHOUT GROWTH FACTORS AND METHODS OF PRIMARY CULTURE AND OF OBTAINING CELL LINES USING THE SAME
DE3801236A1 (en) 1988-01-18 1989-07-27 Boehringer Mannheim Gmbh PENTOSANE SULFATE MEDIUM
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
JPH03504330A (en) 1988-12-14 1991-09-26 アメリカ合衆国 Cell culture medium for human liver epithelial cell lines
US5529920A (en) 1988-12-14 1996-06-25 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Human liver epithelial cell line and culture media therefor
EP1132471A3 (en) 1989-09-12 2001-11-28 F. Hoffmann-La Roche Ag TNF-binding proteins
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5426699A (en) 1993-10-25 1995-06-20 Antec Corporation Method and apparatus for digitizing a scrambled analog video signal
EP0666312A1 (en) 1994-02-08 1995-08-09 Wolfgang A. Renner Process for the improvement of mammalian cell growth
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
US6656466B1 (en) 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
HUP0202974A3 (en) 1995-07-17 2005-05-30 Fraunhofer Ges Forschung Metal-containing ribonucleotide polypeptides
JP4306813B2 (en) 1995-09-19 2009-08-05 アスビオファーマ株式会社 New method for culturing animal cells
US6043092A (en) 1996-03-18 2000-03-28 University Of Pittsburgh Cell culture media for mammalian cells
US6146847A (en) 1996-11-01 2000-11-14 Genespan Corporation Stabilized transient gene expression
CA2318376A1 (en) 1998-01-12 1999-07-15 Betagene, Inc. Compositions and methods for regulated secretion from neuroendocrine cell lines
US6528286B1 (en) 1998-05-29 2003-03-04 Genentech, Inc. Mammalian cell culture process for producing glycoproteins
WO2000057891A1 (en) 1999-03-30 2000-10-05 Trustees Of Boston University Compositions and methods for producing platelets and/or proplatelets from megakaryocytes
AT409379B (en) 1999-06-02 2002-07-25 Baxter Ag MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
JP4723140B2 (en) * 1999-06-08 2011-07-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Modified chimeric polypeptide with improved pharmacokinetic properties
US7070959B1 (en) 1999-06-08 2006-07-04 Regeneron Pharmaceuticals, Inc. Modified chimeric polypeptides with improved pharmacokinetic properties
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
CN1114619C (en) * 1999-06-25 2003-07-16 中国科学院上海细胞生物学研究所 Liver cancer murine monoclone antibody without endogenous immunoglobulin and its preparation and application
US7105348B2 (en) 2000-10-31 2006-09-12 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
ATE325865T1 (en) 2001-01-16 2006-06-15 Regeneron Pharma ISOLATION OF CELLS EXPRESSING SECRETED PROTEINS
US20090137416A1 (en) 2001-01-16 2009-05-28 Regeneron Pharmaceuticals, Inc. Isolating Cells Expressing Secreted Proteins
CN1240300C (en) 2001-03-05 2006-02-08 科学与工业研究会 Process for preparation of protein-hydrolysate from soy flour
EP1321515A1 (en) 2001-12-21 2003-06-25 Ingenium Pharmaceuticals AG Method of culturing cells
CN1180080C (en) * 2002-01-18 2004-12-15 中国科学院上海细胞生物学研究所 Preprartion method of improved hepatoma murine monoclonal antibody
DE60309238T2 (en) 2002-03-08 2007-06-06 Asml Netherlands B.V. Lithographic mask, lithographic apparatus and method of making a device
DK1531666T3 (en) 2002-05-29 2014-01-13 Regeneron Pharma INDUCERABLE EUKARYOT EXPRESSION SYSTEM
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US6924124B1 (en) 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
WO2004104186A1 (en) 2003-05-15 2004-12-02 Wyeth Restricted glucose feed for animal cell culture
US7303694B2 (en) 2003-07-17 2007-12-04 Wisconsin Alumni Research Foundation Liquid crystals with reduced toxicity and applications thereof
WO2005028626A2 (en) 2003-09-18 2005-03-31 Raven Biotechnologies, Inc. Cell culture media
EP1767546B1 (en) 2004-06-08 2012-03-07 Chengdu Kanghong Biotechnologies Co., Ltd. Angiogenesis-inhibiting chimeric protein and the use
US7294484B2 (en) 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
CA2585547A1 (en) 2004-10-29 2006-05-11 Centocor, Inc. Chemically defined media compositions
US20060094104A1 (en) * 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
US8273553B2 (en) 2004-11-02 2012-09-25 Ares Trading S.A. Production of growth hormone in serum-free cell culture medium for mammalian cells
AR058568A1 (en) 2005-12-20 2008-02-13 Bristol Myers Squibb Co METHODS TO PRODUCE A COMPOSITION WITH CTLA4-IG MOLECULES FROM A CROP MEANS
PL3121266T3 (en) * 2006-01-04 2020-07-13 Baxalta Incorporated Oligopeptide-free cell culture media
DE602007008627D1 (en) * 2006-02-14 2010-10-07 Genetix Ltd Cell culture medium
US8216575B2 (en) 2006-03-31 2012-07-10 Chengdu Kanghong Biotechnologies Co., Ltd. Inhibition of neovascularization with a soluble chimeric protein comprising VEGF FLT-1 and KDR domains
PL2041177T3 (en) 2006-06-02 2012-09-28 Regeneron Pharma High affinity antibodies to human il-6 receptor
US7608693B2 (en) 2006-10-02 2009-10-27 Regeneron Pharmaceuticals, Inc. High affinity human antibodies to human IL-4 receptor
SI2078071T1 (en) * 2006-11-08 2015-05-29 Wyeth Llc Rationally designed media for cell culture
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
WO2008154014A2 (en) 2007-06-11 2008-12-18 Amgen Inc. A method for culturing mammalian cells to improve recombinant protein production
MY147651A (en) 2007-07-31 2012-12-31 Regeneron Pharma Human antibodies to human cd20 and method of using thereof
US8309088B2 (en) 2007-08-10 2012-11-13 Regeneron Pharmaceuticals, Inc. Method of treating osteoarthritis with an antibody to NGF
CN100482786C (en) * 2007-12-28 2009-04-29 天津百若克医药生物技术有限责任公司 Human embryo kidney 293 cell amplifying protein-free medium
CN102317440B (en) * 2008-01-09 2014-12-03 塞尔卡有限公司 Improved culture media additive and process for using it
CA2737580A1 (en) * 2008-09-26 2010-04-01 Schering Corporation High titer antibody production
EP2356247B2 (en) 2008-11-12 2019-05-15 Baxalta Incorporated Method of producing serum-free insulin-free factor vii
JO3672B1 (en) 2008-12-15 2020-08-27 Regeneron Pharma High Affinity Human Antibodies to PCSK9
HUE038596T2 (en) 2009-06-02 2018-10-29 Regeneron Pharma Fucosylation-deficient cells
CN101603026B (en) 2009-06-19 2011-01-19 华东理工大学 Animal origin-free low-protein culture medium suitable for animal cell product production
EP3916011A1 (en) 2009-06-26 2021-12-01 Regeneron Pharmaceuticals, Inc. Readily isolated bispecific antibodies with native immunoglobulin format
KR101574056B1 (en) 2009-07-31 2015-12-02 백스터 인터내셔널 인코포레이티드 Cell culture medium for adamts protein expression
WO2011079004A1 (en) * 2009-12-23 2011-06-30 Schering Corporation Cell line 3m
JO3417B1 (en) 2010-01-08 2019-10-20 Regeneron Pharma Stabilized formulations containing anti-interleukin-6 receptor (il-6r) antibodies
RS60983B1 (en) 2010-02-24 2020-11-30 Chiesi Farm Spa Process for production and purification of recombinant lysosomal alpha-mannosidase
AU2011246502B2 (en) 2010-04-26 2014-08-28 Novartis Ag Improved cell cultivation process
JO3340B1 (en) 2010-05-26 2019-03-13 Regeneron Pharma Antibodies to human gdf8
HUE030820T2 (en) 2010-05-28 2017-06-28 Hoffmann La Roche Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
TWI670073B (en) * 2010-07-08 2019-09-01 美商巴克斯歐塔公司 Method of producing recombinant high molecular weight vwf in cell culture
JOP20190250A1 (en) 2010-07-14 2017-06-16 Regeneron Pharma Stabilized formulations containing anti-ngf antibodies
KR20130101034A (en) 2010-08-31 2013-09-12 프리슬랜드 브랜즈 비브이 Culture medium for eukaryotic cells
AR083044A1 (en) 2010-09-27 2013-01-30 Regeneron Pharma ANTI-CD48 ANTIBODIES AND USES OF THE SAME
PL2624865T3 (en) 2010-10-06 2018-11-30 Regeneron Pharmaceuticals, Inc. Stabilized formulations containing anti-interleukin-4 receptor (il-4r) antibodies
JO3756B1 (en) 2010-11-23 2021-01-31 Regeneron Pharma Human antibodies to the glucagon receptor
BR112013018751B1 (en) 2010-12-28 2021-01-05 Chugai Seiyaku Kabushiki Kaisha (Chugai Pharmaceutical Co., Ltd.) method for modulating the level of homogeneity components of a protein, method for producing a desired protein, drug and method for preparing it
AR087329A1 (en) 2011-06-17 2014-03-19 Regeneron Pharma HUMAN ANTIBODIES AGAINST PROTEIN 3 OF HUMAN ANGIOPOIETIN TYPE
EP2780368B1 (en) 2011-11-14 2018-01-03 Regeneron Pharmaceuticals, Inc. Compositions and methods for increasing muscle mass and muscle strength by specifically antagonizing gdf8 and/or activin a
WO2013112438A1 (en) 2012-01-23 2013-08-01 Regeneron Pharmaceuticals, Inc. Stabilized formulations containing anti-ang2 antibodies
WO2013120497A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein
JP6090735B2 (en) 2012-03-02 2017-03-08 国立大学法人山口大学 Serum-free medium for culturing digestive cancer stem cells, and method for proliferating digestive cancer stem cells using the same
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
CA2875294C (en) 2012-04-25 2021-06-08 Riken Cell preparation containing myocardium-committed cell
JO3820B1 (en) 2012-05-03 2021-01-31 Regeneron Pharma Human antibodies to fel d1 and methods of use thereof
IN2014DN10441A (en) 2012-06-21 2015-08-21 Baxter Int
JP2015526430A (en) 2012-08-02 2015-09-10 サノフイ Articles of manufacture containing Aflibercept or ZIV-Aflibercept
JP6309521B2 (en) 2012-08-13 2018-04-11 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. Anti-PCSK9 antibody with pH-dependent binding properties
JOP20200236A1 (en) 2012-09-21 2017-06-16 Regeneron Pharma Anti-cd3 antibodies, bispecific antigen-binding molecules that bind cd3 and cd20, and uses thereof
SG10201901708SA (en) 2012-10-23 2019-03-28 Genzyme Corp Perfusion culturing methods and uses thereof
JO3405B1 (en) 2013-01-09 2019-10-20 Regeneron Pharma ANTI-PDGFR-beta ANTIBODIES AND USES THEREOF
MX367025B (en) 2013-02-22 2019-08-02 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof.
NZ711654A (en) 2013-03-12 2021-07-30 Cmc Icos Biologics Inc Improved recombinant protein expression using a hybrid chef1 promoter
JO3532B1 (en) 2013-03-13 2020-07-05 Regeneron Pharma Anti-il-33 antibodies and uses thereof
TWI659968B (en) 2013-03-14 2019-05-21 再生元醫藥公司 Human antibodies to respiratory syncytial virus f protein and methods of use thereof
WO2014159633A1 (en) 2013-03-14 2014-10-02 Medimmune, Llc Recombinant polypeptide production
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
CA2904377C (en) 2013-03-15 2021-07-13 Regeneron Pharmaceuticals, Inc. Il-33 antagonists and uses thereof
AR095196A1 (en) 2013-03-15 2015-09-30 Regeneron Pharma SERUM FREE CELL CULTIVATION MEDIA
US20160237400A1 (en) 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
EP3004368B1 (en) 2013-05-29 2019-09-18 F.Hoffmann-La Roche Ag Quantitative control of sialylation
TWI641620B (en) 2013-08-21 2018-11-21 再生元醫藥公司 Anti-prlr antibodies and uses thereof
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US10513723B2 (en) 2014-01-13 2019-12-24 Amgen Inc. Decreasing ornithine production to decrease high mannose glycoform content of recombinant proteins
TWI680138B (en) 2014-01-23 2019-12-21 美商再生元醫藥公司 Human antibodies to pd-l1
TWI681969B (en) 2014-01-23 2020-01-11 美商再生元醫藥公司 Human antibodies to pd-1
RS59077B1 (en) 2014-03-11 2019-09-30 Regeneron Pharma Anti-egfrviii antibodies and uses thereof
TWI754319B (en) 2014-03-19 2022-02-01 美商再生元醫藥公司 Methods and antibody compositions for tumor treatment
NZ726602A (en) 2014-05-05 2024-05-31 Regeneron Pharmaceuticals Inc Humanized c5 and c3 animals
JO3701B1 (en) 2014-05-23 2021-01-31 Regeneron Pharma Human antibodies to middle east respiratory syndrome – coronavirus spike protein
KR102576850B1 (en) 2014-05-27 2023-09-11 아카데미아 시니카 Fucosidase from bacteroides and methods using the same
JP2017516484A (en) 2014-06-03 2017-06-22 ルピン・リミテッド Cell culture process for protein production
US9657099B2 (en) 2014-09-16 2017-05-23 Regeneron Pharmaceuticals, Inc. Anti-glucagon antibodies
TWI710573B (en) 2015-01-26 2020-11-21 美商再生元醫藥公司 Human antibodies to ebola virus glycoprotein
SG11201705743YA (en) 2015-04-01 2017-08-30 Boehringer Ingelheim Int Cell culture medium
TW202340452A (en) 2015-08-04 2023-10-16 美商再生元醫藥公司 Taurine supplemented cell culture medium and methods of use
KR101936049B1 (en) 2015-10-15 2019-01-08 (주)알테오젠 Method for manufacturing fusion proteins with IgG Fc domain
TWI734775B (en) 2016-04-26 2021-08-01 美商美國泰福生技股份有限公司 Cell culture medium
SG11201912548XA (en) 2017-07-06 2020-01-30 Regeneron Pharma Cell culture process for making a glycoprotein

Also Published As

Publication number Publication date
HUE040233T2 (en) 2019-02-28
EP2970876B1 (en) 2018-07-11
MX2021008627A (en) 2022-06-14
AU2021205088B2 (en) 2022-09-22
JP2019150037A (en) 2019-09-12
EA037568B1 (en) 2021-04-15
ES2682929T3 (en) 2018-09-24
IL241195B (en) 2019-05-30
KR20210031778A (en) 2021-03-22
JP2016514452A (en) 2016-05-23
TWI629359B (en) 2018-07-11
CA3238121A1 (en) 2014-09-18
CA2906768A1 (en) 2014-09-18
JP2022172390A (en) 2022-11-15
KR102229380B1 (en) 2021-03-18
EP2970876A1 (en) 2016-01-20
PL2970876T3 (en) 2018-11-30
JP7429743B2 (en) 2024-02-08
IL289724A (en) 2022-03-01
EA202190326A3 (en) 2021-09-30
NZ711935A (en) 2021-04-30
AU2020200052A1 (en) 2020-01-30
AU2022211862A1 (en) 2022-08-25
KR102278316B1 (en) 2021-07-19
MX2015013176A (en) 2016-04-07
CN105143444A (en) 2015-12-09
AU2014227804A1 (en) 2015-09-24
KR20220116571A (en) 2022-08-23
IL281515B1 (en) 2024-01-01
BR122020021864B1 (en) 2022-09-20
US20140273095A1 (en) 2014-09-18
JP2021180682A (en) 2021-11-25
MX357977B (en) 2018-08-01
AR095196A1 (en) 2015-09-30
TWI782295B (en) 2022-11-01
EP3919611A1 (en) 2021-12-08
AU2014227804B2 (en) 2020-01-30
SG10202107444XA (en) 2021-08-30
CA3135232C (en) 2024-06-18
PT2970876T (en) 2018-10-22
TW201502273A (en) 2015-01-16
IL289724B2 (en) 2023-08-01
HK1213289A1 (en) 2016-06-30
US20200149081A1 (en) 2020-05-14
AU2021205088A1 (en) 2021-08-12
SG11201507072TA (en) 2015-10-29
US11332771B2 (en) 2022-05-17
CN113481148A (en) 2021-10-08
CA3188491A1 (en) 2014-09-18
EP4159841A1 (en) 2023-04-05
KR20150128785A (en) 2015-11-18
TW201835325A (en) 2018-10-01
US11970724B2 (en) 2024-04-30
TW202041672A (en) 2020-11-16
EA202190326A2 (en) 2021-05-31
KR20240065185A (en) 2024-05-14
EA201591817A1 (en) 2016-05-31
JP2024001357A (en) 2024-01-09
US20210332402A1 (en) 2021-10-28
JP6781039B2 (en) 2020-11-04
IL281515A (en) 2021-04-29
US20220228187A1 (en) 2022-07-21
US20220372436A1 (en) 2022-11-24
BR112015022682A2 (en) 2017-07-18
BR112015022682B1 (en) 2023-03-28
CA3238123A1 (en) 2014-09-18
KR20200119894A (en) 2020-10-20
TW202325723A (en) 2023-07-01
IL264499A (en) 2019-02-28
TWI701333B (en) 2020-08-11
EA202191602A2 (en) 2021-08-31
MY175957A (en) 2020-07-16
CA2906768C (en) 2022-08-30
IL289724B1 (en) 2023-04-01
IL281515B2 (en) 2024-05-01
KR102166166B1 (en) 2020-10-15
IL241195A0 (en) 2015-11-30
KR20210090732A (en) 2021-07-20
EA202191602A3 (en) 2021-12-31
DK2970876T3 (en) 2018-08-13
WO2014144198A1 (en) 2014-09-18
IL300911A (en) 2023-04-01
JP6944969B2 (en) 2021-10-06
EP3378932A1 (en) 2018-09-26
CA3135232A1 (en) 2014-09-18
AU2020200052B2 (en) 2023-01-19
MX2021001859A (en) 2021-04-19

Similar Documents

Publication Publication Date Title
IL289724A (en) Serum-free cell culture medium
IL246262A0 (en) Media for cell culture
SG11201705743YA (en) Cell culture medium
EP2968549A4 (en) Methods of cell culture
EP2971040A4 (en) Methods of cell culture
EP2821477A4 (en) Cell culture device having culture medium replacement function
EP2971014A4 (en) Methods of cell culture
EP3045527A4 (en) Cell culture vessel
HK1221250A1 (en) Structure for culturing cells
SG10201700670WA (en) Cell culture
IL245392B (en) Cell culture method
EP3045526A4 (en) Cell culture vessel
PL2970845T3 (en) Bioreactor
GB201420277D0 (en) Cell culture system
SG11201509767UA (en) Medium for culturing stem cells
SG11201510040VA (en) Serum-free medium containing pdgf for ds cells
GB201211873D0 (en) Cell culture
EP3044306B8 (en) Support for cell cultures
EP3049514A4 (en) Cell culture media
SG11201507338PA (en) Method for culturing cells
GB201308905D0 (en) Cell culture
GB201306488D0 (en) Cell culture
AU2013900676A0 (en) Structure for culturing cells