SG10201402638QA - Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs - Google Patents

Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs

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Publication number
SG10201402638QA
SG10201402638QA SG10201402638QA SG10201402638QA SG10201402638QA SG 10201402638Q A SG10201402638Q A SG 10201402638QA SG 10201402638Q A SG10201402638Q A SG 10201402638QA SG 10201402638Q A SG10201402638Q A SG 10201402638QA SG 10201402638Q A SG10201402638Q A SG 10201402638QA
Authority
SG
Singapore
Prior art keywords
phas
microorganisms
pseudomonas
ipb
lcl
Prior art date
Application number
SG10201402638QA
Inventor
Nica Bassas Gali M
Arias Rivas Sagrario
Molinari Gabriella
Nigel Timmis Kenneth
Original Assignee
Dritte Patentportfolio Beteiligungs Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dritte Patentportfolio Beteiligungs Gmbh & Co Kg filed Critical Dritte Patentportfolio Beteiligungs Gmbh & Co Kg
Publication of SG10201402638QA publication Critical patent/SG10201402638QA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
    • C08G63/02—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds
    • C08G63/06—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from hydroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas

Abstract

23 Abstract Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs 5 The present application is directed at microorganisms of the genus Pseudomonas as deposited under DSM26199 ( Pseudomonas sp. IPB-B26) and DSM26200 ( Pseudomonas sp. N-128) with the Leibnitz Institute DSMZ. The present application is further directed at a process for the production of medium- and long-chain-length PHAs, comprising cultivating said microorganisms in a culture 10 medium comprising a carbon source and isolating the PHA from the microorganisms. It has been observed that these microorganisms allow for efficient PHA production in high yields. In addition, the inventive microorganisms possess the valuable capability to incorporate unsaturated fatty acids into the resulting PHAs. The inventive microorganisms thus enable a later modification of 15 the PHAs as well as cross-linking, thus opening new fields of applications for these materials. [Figure 1]
SG10201402638QA 2013-06-25 2014-05-26 Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs SG10201402638QA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13173572.2A EP2818561A1 (en) 2013-06-25 2013-06-25 Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs

Publications (1)

Publication Number Publication Date
SG10201402638QA true SG10201402638QA (en) 2015-01-29

Family

ID=48669821

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201402638QA SG10201402638QA (en) 2013-06-25 2014-05-26 Utilization of the novel, environmental isolates Pseudomonas sp. IPB-B26 and N-128 for the efficient high yield production of mcl/lcl-PHAs

Country Status (10)

Country Link
US (1) US20140378646A1 (en)
EP (1) EP2818561A1 (en)
JP (1) JP2015006182A (en)
KR (1) KR20150000840A (en)
CN (1) CN104250628A (en)
BR (1) BR102014015395A2 (en)
CA (1) CA2852770A1 (en)
IN (1) IN2014MU02028A (en)
RU (1) RU2014125436A (en)
SG (1) SG10201402638QA (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047741A (en) * 2016-03-04 2016-10-26 李涛 Bacterial strain and synthetic method for synthesizing PHA from chitin, and application of PHA

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10077333B2 (en) * 2016-04-20 2018-09-18 Elevance Renewable Sciences, Inc. Renewably derived polyesters and methods of making and using the same
CN107996057A (en) * 2017-12-05 2018-05-08 中国科学院青岛生物能源与过程研究所 A kind of method for combining soil water-retaining soil moisture conservation and emission reduction methane
CN112251367B (en) * 2019-07-22 2022-03-29 中国科学院微生物研究所 Aerobic denitrification preparation and preparation method and application thereof
CN111440749B (en) * 2020-05-27 2022-02-22 中国科学院城市环境研究所 Pseudomonas pseudoalcaligenes sp.H3, screening method and application
CN112899316B (en) * 2021-02-26 2024-02-20 辽宁大学 Method for producing PHA by using pseudomonas wrinkliniella II type synthase

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876331A (en) 1987-08-18 1989-10-24 Mitsubishi Kasei Corporation Copolyester and process for producing the same
JP2777757B2 (en) 1991-09-17 1998-07-23 鐘淵化学工業株式会社 Copolymer and method for producing the same
EP1752532A1 (en) 2005-08-09 2007-02-14 Helmholtz-Zentrum für Infektionsforschung GmbH Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047741A (en) * 2016-03-04 2016-10-26 李涛 Bacterial strain and synthetic method for synthesizing PHA from chitin, and application of PHA

Also Published As

Publication number Publication date
BR102014015395A2 (en) 2015-10-06
JP2015006182A (en) 2015-01-15
EP2818561A1 (en) 2014-12-31
IN2014MU02028A (en) 2015-10-09
US20140378646A1 (en) 2014-12-25
CN104250628A (en) 2014-12-31
RU2014125436A (en) 2015-12-27
CA2852770A1 (en) 2014-12-25
KR20150000840A (en) 2015-01-05

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