SE9502765D0 - Assay Procedure - Google Patents

Assay Procedure

Info

Publication number
SE9502765D0
SE9502765D0 SE9502765A SE9502765A SE9502765D0 SE 9502765 D0 SE9502765 D0 SE 9502765D0 SE 9502765 A SE9502765 A SE 9502765A SE 9502765 A SE9502765 A SE 9502765A SE 9502765 D0 SE9502765 D0 SE 9502765D0
Authority
SE
Sweden
Prior art keywords
enzyme
nucleotide
primer
activity
template
Prior art date
Application number
SE9502765A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE9502765L (en
SE520315C2 (en
Inventor
Clas Kaellander
Jan-Simon Gronowitz
Johan Lennerstrand
Original Assignee
Clas Kaellander
Jan Simon Gronowitz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clas Kaellander, Jan Simon Gronowitz filed Critical Clas Kaellander
Priority to SE9502765A priority Critical patent/SE520315C2/en
Publication of SE9502765D0 publication Critical patent/SE9502765D0/en
Priority to DE69630135T priority patent/DE69630135T2/en
Priority to PCT/SE1996/000990 priority patent/WO1998006873A1/en
Priority to EP96933690A priority patent/EP0856066B1/en
Priority to AT96933690T priority patent/ATE250672T1/en
Publication of SE9502765L publication Critical patent/SE9502765L/en
Publication of SE520315C2 publication Critical patent/SE520315C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method for determining the activity of a nucleotide-polymerising enzyme in a sample comprises: (a) capturing the enzyme with a monoclonal antibody (MAb) immobilised on a fixed carrier (the MAb binds the enzyme without significantly affecting its activity); (b) removing impurities and/or disturbance factors; (c) starting nucleotide polymerisation by adding a reaction soln. contg. a primer/template construction and nucleotide substrate; (d) providing reaction conditions to favour permanent association between the enzyme and primer/template prod. complex; (e) washing the nucleotide substrate, primer/template and reaction soln. to remove freshly synthesised polymer; (f) measuring the amt. of nucleotide incorporated with the polymer by standard techniques, and (g) determining enzyme activity.
SE9502765A 1995-08-04 1995-08-04 Measuring DNA polymerising enzyme activity in body fluids, cells or virus infected cultures SE520315C2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
SE9502765A SE520315C2 (en) 1995-08-04 1995-08-04 Measuring DNA polymerising enzyme activity in body fluids, cells or virus infected cultures
DE69630135T DE69630135T2 (en) 1995-08-04 1996-08-12 METHOD FOR DETECTING THE ACTIVITY OF DNA POLYMERASES
PCT/SE1996/000990 WO1998006873A1 (en) 1995-08-04 1996-08-12 Method for determining activity of reverse transcriptase
EP96933690A EP0856066B1 (en) 1995-08-04 1996-08-12 Method for determining activity of dna polymerases
AT96933690T ATE250672T1 (en) 1995-08-04 1996-08-12 METHOD FOR DETECTING THE ACTIVITY OF DNA POLYMERASES

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9502765A SE520315C2 (en) 1995-08-04 1995-08-04 Measuring DNA polymerising enzyme activity in body fluids, cells or virus infected cultures

Publications (3)

Publication Number Publication Date
SE9502765D0 true SE9502765D0 (en) 1995-08-04
SE9502765L SE9502765L (en) 1997-02-05
SE520315C2 SE520315C2 (en) 2003-06-24

Family

ID=20399122

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9502765A SE520315C2 (en) 1995-08-04 1995-08-04 Measuring DNA polymerising enzyme activity in body fluids, cells or virus infected cultures

Country Status (1)

Country Link
SE (1) SE520315C2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110832083A (en) * 2017-07-04 2020-02-21 卡维迪有限公司 Method for evaluating sensitivity of virus to treatment by measuring enzyme activity and system thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110832083A (en) * 2017-07-04 2020-02-21 卡维迪有限公司 Method for evaluating sensitivity of virus to treatment by measuring enzyme activity and system thereof

Also Published As

Publication number Publication date
SE9502765L (en) 1997-02-05
SE520315C2 (en) 2003-06-24

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