SE9501939D0 - DNA molecules for expression of polypeptides - Google Patents

DNA molecules for expression of polypeptides

Info

Publication number
SE9501939D0
SE9501939D0 SE9501939A SE9501939A SE9501939D0 SE 9501939 D0 SE9501939 D0 SE 9501939D0 SE 9501939 A SE9501939 A SE 9501939A SE 9501939 A SE9501939 A SE 9501939A SE 9501939 D0 SE9501939 D0 SE 9501939D0
Authority
SE
Sweden
Prior art keywords
polypeptide
pichia pastoris
coding
expression
region
Prior art date
Application number
SE9501939A
Other languages
Swedish (sv)
Inventor
Goutam Das
Original Assignee
Astra Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=20398427&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SE9501939(D0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Astra Ab filed Critical Astra Ab
Priority to SE9501939A priority Critical patent/SE9501939D0/en
Publication of SE9501939D0 publication Critical patent/SE9501939D0/en
Priority to AU51656/96A priority patent/AU5165696A/en
Priority to BR9607920A priority patent/BR9607920A/en
Priority to CZ972973A priority patent/CZ297397A3/en
Priority to IE960211A priority patent/IE960211A1/en
Priority to TR97/01010T priority patent/TR199701010T1/en
Priority to PCT/SE1996/000318 priority patent/WO1996037622A1/en
Priority to PL96322848A priority patent/PL322848A1/en
Priority to EP96908415A priority patent/EP0832257A1/en
Priority to RU97117367/13A priority patent/RU2157847C2/en
Priority to SK1256-97A priority patent/SK125697A3/en
Priority to HU9802388A priority patent/HUP9802388A3/en
Priority to CN96194083A priority patent/CN1185812A/en
Priority to JP08535592A priority patent/JP2000510683A/en
Priority to EE9700321A priority patent/EE9700321A/en
Priority to KR1019970706642A priority patent/KR19980703238A/en
Priority to AU48033/96A priority patent/AU715297B2/en
Priority to NZ286234A priority patent/NZ286234A/en
Priority to GB9606023A priority patent/GB2299085B/en
Priority to CA002172447A priority patent/CA2172447A1/en
Priority to AR33588496A priority patent/AR001410A1/en
Priority to TW085104253A priority patent/TW434314B/en
Priority to IL11833596A priority patent/IL118335A0/en
Priority to NO974318A priority patent/NO974318L/en
Priority to HK98111871A priority patent/HK1011048A1/en
Priority to US09/418,176 priority patent/US20030040040A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to DNA molecules, recombinant vectors and cell cultures for use in methods for expression of bile salt-stimulated lipase (BSSL) in the methylotrophic yeast Pichia pastoris. The DNA molecule comprising: (a) a region coding for a polypeptide which is human BSSL or a biologically active variant thereof; (b) joined to the 5'-end of said polypeptide coding region, a region coding for a signal peptide capable of directing secretion of said polypeptide from Pichia pastoris cells transformed with said DNA molecule; and (c) operably-linked to said coding regions defined in (a) and (b), the methanol oxidase promoter of Pichia pastoris or a functionally equivalent promoter.
SE9501939A 1995-03-23 1995-05-24 DNA molecules for expression of polypeptides SE9501939D0 (en)

Priority Applications (26)

Application Number Priority Date Filing Date Title
SE9501939A SE9501939D0 (en) 1995-05-24 1995-05-24 DNA molecules for expression of polypeptides
KR1019970706642A KR19980703238A (en) 1995-03-23 1996-03-12 DNA molecules for expression of the succinate-stimulated lipase (BSSL)
EP96908415A EP0832257A1 (en) 1995-05-24 1996-03-12 A dna molecule for expression of bile salt-stimulated lipase (bssl)
HU9802388A HUP9802388A3 (en) 1995-05-24 1996-03-12 A dna molecule for expression of bile salt-stimulated lipase (bssl)
EE9700321A EE9700321A (en) 1995-05-24 1996-03-12 A DNA molecule for the expression of lipase stimulated with bile acid salts
CZ972973A CZ297397A3 (en) 1995-05-24 1996-03-12 Dna molecule for expression of bile salt stimulated lipase (bssl)
IE960211A IE960211A1 (en) 1995-03-23 1996-03-12 Dna molecules for expression of polypeptides
TR97/01010T TR199701010T1 (en) 1995-05-24 1996-03-12 DNA for bile salt-stimulated lipazine expression.
PCT/SE1996/000318 WO1996037622A1 (en) 1995-05-24 1996-03-12 A dna molecule for expression of bile salt-stimulated lipase (bssl)
PL96322848A PL322848A1 (en) 1995-05-24 1996-03-12 Dna molecule for expression of bile salt stimulated lipase (bssl)
BR9607920A BR9607920A (en) 1995-03-23 1996-03-12 DNA molecule of expression replicable host cells of the genus pichia and process for the production of a polypeptide that is a human bssl
RU97117367/13A RU2157847C2 (en) 1995-05-24 1996-03-12 Dna molecule for expression of bile salt-stimulated lipase
SK1256-97A SK125697A3 (en) 1995-03-23 1996-03-12 A dna molecule for expression of bile salt-stimulated lipase (bssl)
AU51656/96A AU5165696A (en) 1995-05-24 1996-03-12 A dna molecule for expression of bile salt-stimulated lipase (bssl)
CN96194083A CN1185812A (en) 1995-05-24 1996-03-12 A DNA molecule for expression of bile salt-stimulated lipase (BSSL)
JP08535592A JP2000510683A (en) 1995-05-24 1996-03-12 DNA molecule for bile salt-stimulated lipase (BSSL) expression
AU48033/96A AU715297B2 (en) 1995-03-23 1996-03-13 DNA molecules for expression of polypeptides
NZ286234A NZ286234A (en) 1995-03-23 1996-03-21 Expression of recombinant bile salt-stimulated lipase (bssl)
AR33588496A AR001410A1 (en) 1995-03-23 1996-03-22 DNA molecules for the expression of polypeptides, a vector that contains them, host cells and procedure for the production of a polypeptide.
GB9606023A GB2299085B (en) 1995-03-23 1996-03-22 Expression of bile salt-stimulated lipase in the yeast Pichia
CA002172447A CA2172447A1 (en) 1995-03-23 1996-03-22 Dna molecules for expression of polypeptides
TW085104253A TW434314B (en) 1995-05-24 1996-04-10 DNA molecules for expression of polypeptides
IL11833596A IL118335A0 (en) 1995-05-24 1996-05-20 DNA molecules for expression of polypeptides
NO974318A NO974318L (en) 1995-03-23 1997-09-19 DNA molecule for expression of bile salt-stimulated lipase (BSSL)
HK98111871A HK1011048A1 (en) 1995-03-23 1998-11-10 Expression of bile salt-stimulated lipase in the yeast pichia
US09/418,176 US20030040040A1 (en) 1995-03-23 1999-10-13 Dna molecules for expression of bile salt-stimulated lipase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9501939A SE9501939D0 (en) 1995-05-24 1995-05-24 DNA molecules for expression of polypeptides

Publications (1)

Publication Number Publication Date
SE9501939D0 true SE9501939D0 (en) 1995-05-24

Family

ID=20398427

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9501939A SE9501939D0 (en) 1995-03-23 1995-05-24 DNA molecules for expression of polypeptides

Country Status (15)

Country Link
EP (1) EP0832257A1 (en)
JP (1) JP2000510683A (en)
KR (1) KR19980703238A (en)
CN (1) CN1185812A (en)
AU (1) AU5165696A (en)
CZ (1) CZ297397A3 (en)
EE (1) EE9700321A (en)
HU (1) HUP9802388A3 (en)
IL (1) IL118335A0 (en)
PL (1) PL322848A1 (en)
RU (1) RU2157847C2 (en)
SE (1) SE9501939D0 (en)
TR (1) TR199701010T1 (en)
TW (1) TW434314B (en)
WO (1) WO1996037622A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001255501A1 (en) * 2000-04-21 2001-11-07 Monsanto Technology Llc Blood-pressure reducing polypeptides containing vpp derived from microorganisms
KR100717353B1 (en) * 2006-04-12 2007-05-11 한국생명공학연구원 Auto-inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it
CN103952386A (en) * 2014-03-31 2014-07-30 四川农业大学 Efficient secretory expression method of recombinant porcine pancreatic lipases PPL by using pichia pastoris
RU2697218C1 (en) * 2018-07-11 2019-08-13 федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" Use of mitochondrial localization signal peptides for increasing level of heterologous expression of proteins in pichia pastoris and saccharomyces cerevisiae
BR112022000391A2 (en) * 2019-07-12 2022-03-03 Lipum Ab new bssl antibodies

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0438200B1 (en) * 1990-01-16 2002-07-17 Centro De Ingenieria Genetica Y Biotecnologia Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
IS4130A (en) * 1993-03-01 1994-09-02 Ab Astra New polypeptide

Also Published As

Publication number Publication date
PL322848A1 (en) 1998-02-16
KR19980703238A (en) 1998-10-15
RU2157847C2 (en) 2000-10-20
HUP9802388A3 (en) 2000-10-30
CZ297397A3 (en) 1998-03-18
TR199701010T1 (en) 1998-01-21
EP0832257A1 (en) 1998-04-01
CN1185812A (en) 1998-06-24
IL118335A0 (en) 1996-09-12
HUP9802388A2 (en) 1999-02-01
EE9700321A (en) 1998-06-15
TW434314B (en) 2001-05-16
AU5165696A (en) 1996-12-11
JP2000510683A (en) 2000-08-22
WO1996037622A1 (en) 1996-11-28

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