SE9301313L - Imaging fluorescence device with tunable detection - Google Patents

Imaging fluorescence device with tunable detection

Info

Publication number
SE9301313L
SE9301313L SE9301313A SE9301313A SE9301313L SE 9301313 L SE9301313 L SE 9301313L SE 9301313 A SE9301313 A SE 9301313A SE 9301313 A SE9301313 A SE 9301313A SE 9301313 L SE9301313 L SE 9301313L
Authority
SE
Sweden
Prior art keywords
optical filter
recording
tunable
fluorescence radiation
weighted signal
Prior art date
Application number
SE9301313A
Other languages
Swedish (sv)
Other versions
SE9301313D0 (en
Inventor
Sune Roland Svanberg
Carl Sune Haakan Montan
Original Assignee
Spektraphos Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spektraphos Ab filed Critical Spektraphos Ab
Priority to SE9301313A priority Critical patent/SE9301313L/en
Publication of SE9301313D0 publication Critical patent/SE9301313D0/en
Priority to PCT/SE1994/000352 priority patent/WO1994024529A1/en
Publication of SE9301313L publication Critical patent/SE9301313L/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type
    • G01N2021/1772—Array detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

Abstract

The present invention is a fluorescence imaging system comprising a light source (1) for irradiating an object (2), an optical filter (3) for separating fluorescence radiation emitted by said object and detector means (5) to generate a respective signal for each image point and a recording and computing unit for the recording of said fluorescence radiation in a plurality of wavelength bands and executing a mathematical and/or logic operation on signals on the same image point corresponding to one and the same point on the object, therewith to obtain an expression for a weighted signal value and to produce an image of the object from the weighted signal values for the different points of the object; said optical filter being tunable to an arbitrary wavelength passband within a large wavelength range. The optical filter is an electronically tunable interference filter.
SE9301313A 1993-04-21 1993-04-21 Imaging fluorescence device with tunable detection SE9301313L (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE9301313A SE9301313L (en) 1993-04-21 1993-04-21 Imaging fluorescence device with tunable detection
PCT/SE1994/000352 WO1994024529A1 (en) 1993-04-21 1994-04-20 Fluorescence imaging with tunable detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9301313A SE9301313L (en) 1993-04-21 1993-04-21 Imaging fluorescence device with tunable detection

Publications (2)

Publication Number Publication Date
SE9301313D0 SE9301313D0 (en) 1993-04-21
SE9301313L true SE9301313L (en) 1994-10-22

Family

ID=20389640

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9301313A SE9301313L (en) 1993-04-21 1993-04-21 Imaging fluorescence device with tunable detection

Country Status (2)

Country Link
SE (1) SE9301313L (en)
WO (1) WO1994024529A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19804532A1 (en) * 1998-02-05 1999-08-12 Herolab Gmbh Device for the optical examination of samples
US6140653A (en) * 1998-03-27 2000-10-31 Vysis, Inc. Large-field fluorescence imaging apparatus
CN105424668A (en) * 2015-12-22 2016-03-23 深圳先进技术研究院 Fluorescent screening system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE455646B (en) * 1984-10-22 1988-07-25 Radians Innova Ab FLUORESCENT DEVICE
US4859063A (en) * 1986-02-11 1989-08-22 University Of Massachusetts Medical Center Imaging microspectrofluorimeter

Also Published As

Publication number Publication date
WO1994024529A1 (en) 1994-10-27
SE9301313D0 (en) 1993-04-21

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