SE9103427D0 - PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE - Google Patents

PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE

Info

Publication number
SE9103427D0
SE9103427D0 SE9103427A SE9103427A SE9103427D0 SE 9103427 D0 SE9103427 D0 SE 9103427D0 SE 9103427 A SE9103427 A SE 9103427A SE 9103427 A SE9103427 A SE 9103427A SE 9103427 D0 SE9103427 D0 SE 9103427D0
Authority
SE
Sweden
Prior art keywords
fluorescence
measured
standard curve
signal
substance
Prior art date
Application number
SE9103427A
Other languages
Swedish (sv)
Inventor
H Drevin
Original Assignee
Pharmacia Biosystems Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biosystems Ab filed Critical Pharmacia Biosystems Ab
Priority to SE9103427A priority Critical patent/SE9103427D0/en
Publication of SE9103427D0 publication Critical patent/SE9103427D0/en
Priority to AU30535/92A priority patent/AU3053592A/en
Priority to PCT/SE1992/000792 priority patent/WO1993010437A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method of measuring fluorescence emitted by a fluorophore when assaying a substance (analyte) in a sample, wherein a measured florescence signal is compared with a standard curve in which signal strenght (emission) is a function of the analyte concentration. The method is mainly characterized in that (i) the fluorescence values which correspond to the analyte concentration interval (c) of the standard curve are measured at the same emission wavelength (lambdaem) or at the same excitation wavelength (lambdaex) wherein the interval (c) is divided into a finite number of part-intervals C1,.. Ca,... Cn, where a &cir& - is an integer 1 </= a </= n and n &cir& - is an integer >/= 2 and (ii) that the signal (fluorescence)emitted from the sample is measured for the excitation wavelength and at the emission wavelength respectively which correspond to the wavelengths used when measuring corresponding values on the standard curve.
SE9103427A 1991-11-20 1991-11-20 PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE SE9103427D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE9103427A SE9103427D0 (en) 1991-11-20 1991-11-20 PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE
AU30535/92A AU3053592A (en) 1991-11-20 1992-11-18 A method relating to the quantification of a substance with the aid of fluorescence
PCT/SE1992/000792 WO1993010437A1 (en) 1991-11-20 1992-11-18 A method relating to the quantification of a substance with the aid of fluorescence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9103427A SE9103427D0 (en) 1991-11-20 1991-11-20 PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE

Publications (1)

Publication Number Publication Date
SE9103427D0 true SE9103427D0 (en) 1991-11-20

Family

ID=20384375

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9103427A SE9103427D0 (en) 1991-11-20 1991-11-20 PROCEDURES FOR QUANTIFYING A SUBSTANCE WITH FLUORESCENCE

Country Status (3)

Country Link
AU (1) AU3053592A (en)
SE (1) SE9103427D0 (en)
WO (1) WO1993010437A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780850A (en) * 1996-03-05 1998-07-14 Texaco Inc. API estimate using multiple fluorescence measurements
GB9621122D0 (en) * 1996-10-10 1996-11-27 Cambridge Imaging Ltd Improvements in and relating to methods and apparatus for assay analysis
CN116924596B (en) * 2023-05-30 2024-06-07 深圳市新西林园林景观有限公司 Electrochemical device for sponge urban sewage treatment and treatment method thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4628026A (en) * 1983-11-15 1986-12-09 Dietlind Gardell Method and apparatus for automated double fluorochromization analysis in lymphocytotoxicity testing
US4745285A (en) * 1986-08-21 1988-05-17 Becton Dickinson And Company Multi-color fluorescence analysis with single wavelength excitation
US4783314A (en) * 1987-02-26 1988-11-08 Nalco Chemical Company Fluorescent tracers - chemical treatment monitors
US5094959A (en) * 1989-04-26 1992-03-10 Foxs Labs Method and material for measurement of oxygen concentration

Also Published As

Publication number Publication date
AU3053592A (en) 1993-06-15
WO1993010437A1 (en) 1993-05-27

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