SE8803689L - METHOD ALSO DETECTING DIFFERENT TYPES OF ANTIBODIES AND / OR ANTIGENS - Google Patents

METHOD ALSO DETECTING DIFFERENT TYPES OF ANTIBODIES AND / OR ANTIGENS

Info

Publication number
SE8803689L
SE8803689L SE8803689A SE8803689A SE8803689L SE 8803689 L SE8803689 L SE 8803689L SE 8803689 A SE8803689 A SE 8803689A SE 8803689 A SE8803689 A SE 8803689A SE 8803689 L SE8803689 L SE 8803689L
Authority
SE
Sweden
Prior art keywords
antibodies
antigens
different types
bound
added
Prior art date
Application number
SE8803689A
Other languages
Swedish (sv)
Other versions
SE465943B (en
SE8803689D0 (en
Inventor
Conny Lilja
Original Assignee
Virovahl Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virovahl Sa filed Critical Virovahl Sa
Priority to SE8803689A priority Critical patent/SE465943B/en
Publication of SE8803689D0 publication Critical patent/SE8803689D0/en
Priority to KR1019900701302A priority patent/KR900702371A/en
Priority to EP89911411A priority patent/EP0438457A1/en
Priority to PCT/SE1989/000565 priority patent/WO1990004182A1/en
Priority to JP1510624A priority patent/JPH05504192A/en
Priority to AU43469/89A priority patent/AU4346989A/en
Publication of SE8803689L publication Critical patent/SE8803689L/en
Publication of SE465943B publication Critical patent/SE465943B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Method for detecting antibodies and/or antigens secreted and/or released by individual cells, a cell suspension being brought into contact with corresponding antigens and/or antibodies bound to a solid carrier. Subsequently or simultaneously, at least two different types of other antibodies intended for and capable of being bound to the antibodies or antigens to be detected are added, which other antibodies are provided with different enzymes. A corresponding number of indicator substances having a distinguishing colouring effect is then added for simultaneous detection of the different types of antibodies and/or antigens by evaluation of patches with a differentiating colour remaining on the solid carrier. According to one embodiment, use is made of two solid carriers, antigens and/or antibodies of the same or different type being bound to opposite surfaces of the carriers and the cell suspension being introduced between these surfaces. <IMAGE>
SE8803689A 1988-10-14 1988-10-14 Method for simultaneous detection of different analytes, in particular antibodies, using the "ELISPOT" technique SE465943B (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
SE8803689A SE465943B (en) 1988-10-14 1988-10-14 Method for simultaneous detection of different analytes, in particular antibodies, using the "ELISPOT" technique
KR1019900701302A KR900702371A (en) 1988-10-14 1989-10-13 How to Detect Different Kinds of Antibodies and / or Antigens Simultaneously
EP89911411A EP0438457A1 (en) 1988-10-14 1989-10-13 A method for simultaneous detection of different types of antibodies and/or antigens produced by individual cells
PCT/SE1989/000565 WO1990004182A1 (en) 1988-10-14 1989-10-13 A method for simultaneous detection of different types of antibodies and/or antigens produced by individual cells
JP1510624A JPH05504192A (en) 1988-10-14 1989-10-13 Method for simultaneously detecting various types of antibodies and/or antigens produced by individual cells
AU43469/89A AU4346989A (en) 1988-10-14 1989-10-13 A method for simultaneous detection of different types of antibodies and/or antigens produced by individual cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8803689A SE465943B (en) 1988-10-14 1988-10-14 Method for simultaneous detection of different analytes, in particular antibodies, using the "ELISPOT" technique

Publications (3)

Publication Number Publication Date
SE8803689D0 SE8803689D0 (en) 1988-10-14
SE8803689L true SE8803689L (en) 1990-04-15
SE465943B SE465943B (en) 1991-11-18

Family

ID=20373648

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8803689A SE465943B (en) 1988-10-14 1988-10-14 Method for simultaneous detection of different analytes, in particular antibodies, using the "ELISPOT" technique

Country Status (2)

Country Link
JP (1) JPH05504192A (en)
SE (1) SE465943B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0409775D0 (en) * 2004-04-30 2004-06-09 Mabtech Ab Assay

Also Published As

Publication number Publication date
JPH05504192A (en) 1993-07-01
SE465943B (en) 1991-11-18
SE8803689D0 (en) 1988-10-14

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