SE8703733L - METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM - Google Patents
METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEMInfo
- Publication number
- SE8703733L SE8703733L SE8703733A SE8703733A SE8703733L SE 8703733 L SE8703733 L SE 8703733L SE 8703733 A SE8703733 A SE 8703733A SE 8703733 A SE8703733 A SE 8703733A SE 8703733 L SE8703733 L SE 8703733L
- Authority
- SE
- Sweden
- Prior art keywords
- ligand
- oil
- free
- present
- analysis
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention is based on the fact that an oil, e.g. corn oil, is not soluble in aqueous sample, e.g. a plasma sample. If the aqueous sample contains proteins which are natural binders for a ligand which have hydrophobic properties, then the ligand is present in the free state in both the oil phase and the water phase, while bound ligand is only present in the water phase. If a labeled version of the ligand is present in the system, it is possible to determine the percentage of free ligand in the aqueous sample under equilibrium conditions, provided that the partition coefficient of free ligand also is determined. The percentage of free ligand, together with the total concentration of the ligand, yields the free concentration of the ligand.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8703733A SE8703733L (en) | 1987-09-28 | 1987-09-28 | METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM |
AU25321/88A AU2532188A (en) | 1987-09-28 | 1988-09-28 | Assay for determining the percentage of free substances in biological fluids |
PCT/SE1988/000500 WO1989003043A1 (en) | 1987-09-28 | 1988-09-28 | Assay for determining the percentage of free substances in biological fluids |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8703733A SE8703733L (en) | 1987-09-28 | 1987-09-28 | METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM |
Publications (2)
Publication Number | Publication Date |
---|---|
SE8703733D0 SE8703733D0 (en) | 1987-09-28 |
SE8703733L true SE8703733L (en) | 1989-03-29 |
Family
ID=20369691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8703733A SE8703733L (en) | 1987-09-28 | 1987-09-28 | METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2532188A (en) |
SE (1) | SE8703733L (en) |
WO (1) | WO1989003043A1 (en) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL56839A0 (en) * | 1979-03-11 | 1979-05-31 | Technion Res & Dev Foundation | Improvement in specific binding assay technique |
GB2078370A (en) * | 1980-06-13 | 1982-01-06 | Nat Res Dev | Binding assays |
ATE69507T1 (en) * | 1985-08-21 | 1991-11-15 | Biotope Inc | METHODS AND EQUIPMENT FOR SEPARATION, MIXING AND DETERMINATION OF COMPONENTS IN SPECIFIC BINDING ASSAYS. |
-
1987
- 1987-09-28 SE SE8703733A patent/SE8703733L/en not_active Application Discontinuation
-
1988
- 1988-09-28 AU AU25321/88A patent/AU2532188A/en not_active Abandoned
- 1988-09-28 WO PCT/SE1988/000500 patent/WO1989003043A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
SE8703733D0 (en) | 1987-09-28 |
WO1989003043A1 (en) | 1989-04-06 |
AU2532188A (en) | 1989-04-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
NAV | Patent application has lapsed |
Ref document number: 8703733-9 Effective date: 19900401 |