SE8204306L - PREPARATION OF ASPARTAS - Google Patents

PREPARATION OF ASPARTAS

Info

Publication number
SE8204306L
SE8204306L SE8204306A SE8204306A SE8204306L SE 8204306 L SE8204306 L SE 8204306L SE 8204306 A SE8204306 A SE 8204306A SE 8204306 A SE8204306 A SE 8204306A SE 8204306 L SE8204306 L SE 8204306L
Authority
SE
Sweden
Prior art keywords
aspartase
cell suspension
clarified solution
aspartic acid
solution
Prior art date
Application number
SE8204306A
Other languages
Swedish (sv)
Other versions
SE8204306D0 (en
Inventor
D C Sternberg
L J Moser
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of SE8204306D0 publication Critical patent/SE8204306D0/en
Publication of SE8204306L publication Critical patent/SE8204306L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Aspartic acid is enzymatically produced from ammonium fumarate in the presence of the enzyme, aspartase. A cell-free aspartase solution is prepared by forming an aqueous cell suspension of aspartase- containing microbial cells; incubating the cell suspension, centrifuging the cell suspension to form a clarified solution that contains a major portion of the aspartase activity originally present in the cells, a sufficient amount of an aspartase-stabilizing salt being included in the cell suspension or to the clarified solution, to make the clarified solution hypertonic. Aspartase recovered in this manner is particularly useful in immobilized enzyme reactors for the production of aspartic acid.
SE8204306A 1981-10-15 1982-07-13 PREPARATION OF ASPARTAS SE8204306L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31161881A 1981-10-15 1981-10-15

Publications (2)

Publication Number Publication Date
SE8204306D0 SE8204306D0 (en) 1982-07-13
SE8204306L true SE8204306L (en) 1983-04-16

Family

ID=23207701

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8204306A SE8204306L (en) 1981-10-15 1982-07-13 PREPARATION OF ASPARTAS

Country Status (19)

Country Link
JP (1) JPS5867184A (en)
AU (1) AU8592482A (en)
BE (1) BE893838A (en)
BR (1) BR8204099A (en)
DD (1) DD207219A5 (en)
DE (1) DE3226532A1 (en)
DK (1) DK307582A (en)
ES (1) ES514018A0 (en)
FI (1) FI823502L (en)
FR (1) FR2514782A1 (en)
GB (1) GB2108128A (en)
GR (1) GR78398B (en)
IL (1) IL66262A0 (en)
IT (1) IT8267896A0 (en)
LU (1) LU84273A1 (en)
NL (1) NL8202854A (en)
PL (1) PL238624A1 (en)
SE (1) SE8204306L (en)
ZA (1) ZA824805B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1210719A (en) * 1982-08-13 1986-09-02 Stanley N. Cohen Method of immobilizing enzymes
JPS59113895A (en) * 1982-12-03 1984-06-30 Tanabe Seiyaku Co Ltd Preparation of l-aspartic acid
FR2695638B1 (en) * 1992-09-15 1995-04-07 Rhone Poulenc Chimie Process for the preparation of L-aspartic acid via ammonium aspartate.
EP0798377B1 (en) * 1996-03-29 2001-05-23 Kyowa Hakko Kogyo Co., Ltd. Process for producing aspartase and l-aspartic acid

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5617073B2 (en) * 1971-10-28 1981-04-20
FR2197979A1 (en) * 1972-09-07 1974-03-29 Orsan Amino-transferase producing strain pseudomonas PO 7111 - for prodn of aspartic acid from fumaric acid
IT1023343B (en) * 1974-11-21 1978-05-10 Liquichimica Spa METHOD OF PRODUCTION OF ASPARTIC ACID BY FERMENTATION OF HYDROCARBONS
JPS5675097A (en) * 1979-11-27 1981-06-20 Kyowa Hakko Kogyo Co Ltd Thermophilic aspartase and its preparation

Also Published As

Publication number Publication date
DD207219A5 (en) 1984-02-22
SE8204306D0 (en) 1982-07-13
BR8204099A (en) 1983-07-05
GB2108128A (en) 1983-05-11
ZA824805B (en) 1983-04-27
PL238624A1 (en) 1983-05-09
BE893838A (en) 1982-11-03
FI823502L (en) 1983-04-16
ES8400142A1 (en) 1983-10-16
IT8267896A0 (en) 1982-07-14
FI823502A0 (en) 1982-10-14
NL8202854A (en) 1983-05-02
LU84273A1 (en) 1983-02-07
JPS5867184A (en) 1983-04-21
DE3226532A1 (en) 1983-09-01
DK307582A (en) 1983-04-16
ES514018A0 (en) 1983-10-16
FR2514782A1 (en) 1983-04-22
IL66262A0 (en) 1982-11-30
GR78398B (en) 1984-09-27
AU8592482A (en) 1983-04-21

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