SE7902140L - PRE-TREATMENT OF MICROBERRIES - Google Patents

PRE-TREATMENT OF MICROBERRIES

Info

Publication number
SE7902140L
SE7902140L SE7902140A SE7902140A SE7902140L SE 7902140 L SE7902140 L SE 7902140L SE 7902140 A SE7902140 A SE 7902140A SE 7902140 A SE7902140 A SE 7902140A SE 7902140 L SE7902140 L SE 7902140L
Authority
SE
Sweden
Prior art keywords
cells
beads
microberries
treatment
microcarriers
Prior art date
Application number
SE7902140A
Other languages
Unknown language ( )
Swedish (sv)
Inventor
E M Scattergood
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of SE7902140L publication Critical patent/SE7902140L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

Commercial beads of a dextran matrix to which is bound diethylaminoethyl anion-exchange groups (to make them suitable for use as microcarriers for mass culture of vertebrate anchorage dependent cells such as primary and diploid cells) are pretreated by being soaked in fetal calf serum (FCS) at 75-90 DEG C for about 10 minutes prior to culturing cells on the beads.
SE7902140A 1978-03-10 1979-03-09 PRE-TREATMENT OF MICROBERRIES SE7902140L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US88513778A 1978-03-10 1978-03-10

Publications (1)

Publication Number Publication Date
SE7902140L true SE7902140L (en) 1979-09-11

Family

ID=25386226

Family Applications (1)

Application Number Title Priority Date Filing Date
SE7902140A SE7902140L (en) 1978-03-10 1979-03-09 PRE-TREATMENT OF MICROBERRIES

Country Status (12)

Country Link
JP (1) JPS54129184A (en)
BE (1) BE874740A (en)
CH (1) CH640264A5 (en)
DE (1) DE2909340A1 (en)
DK (1) DK98679A (en)
FR (1) FR2419321A1 (en)
GB (1) GB2016044B (en)
IE (1) IE48321B1 (en)
IT (1) IT7948280A0 (en)
LU (1) LU81018A1 (en)
NL (1) NL7901916A (en)
SE (1) SE7902140L (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2470794A1 (en) * 1979-12-05 1981-06-12 Pasteur Institut NOVEL MICROPARTICLES, THEIR PREPARATION AND THEIR APPLICATIONS IN BIOLOGY, PARTICULARLY TO THE CULTURE OF HUMAN DIPLOID CELLS
FR2543158B1 (en) * 1983-03-24 1985-11-15 Inst Nat Sante Rech Med MEDIUM FOR CULTURING ANIMAL CELLS WITHOUT SERUM, WITHOUT HORMONES AND WITHOUT GROWTH FACTORS AND METHODS OF PRIMARY CULTURE AND OF OBTAINING CELL LINES USING THE SAME

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4036693A (en) * 1976-02-02 1977-07-19 Massachusetts Institute Of Technology Treatment of cell culture microcarries

Also Published As

Publication number Publication date
CH640264A5 (en) 1983-12-30
IT7948280A0 (en) 1979-03-09
GB2016044B (en) 1982-05-19
DK98679A (en) 1979-09-11
IE790598L (en) 1979-09-10
GB2016044A (en) 1979-09-19
BE874740A (en) 1979-09-10
NL7901916A (en) 1979-09-12
JPS54129184A (en) 1979-10-06
LU81018A1 (en) 1979-10-30
IE48321B1 (en) 1984-12-12
FR2419321A1 (en) 1979-10-05
DE2909340A1 (en) 1979-09-13
FR2419321B1 (en) 1984-02-24

Similar Documents

Publication Publication Date Title
ATE6163T1 (en) MANUFACTURE OF ISOMALTULOSE.
BR8002025A (en) PROCESS OF INCLUSION OF MICROORGANISMS IN A POLYMER MATRIX
FR2293439A1 (en) NEW ENZYME CHROMOGENOUS SUBSTRATES
ES465059A1 (en) Production of specific immune nucleic acids cell dialysates and antibodies
SE7712376L (en) ADAPTION OF CELL LINES TO SUSPENSION CULTURES
SE7902140L (en) PRE-TREATMENT OF MICROBERRIES
ES8106552A1 (en) Process for propagating human hepatitis A virus in in vitro cell culture.
ATE45766T1 (en) PROPAGATION PROCEDURES FOR PLANT CELL ASSOCIATIONS.
SE8303925D0 (en) INTERFERON E
PL214144A1 (en) METHOD OF FIXING THE GLASS PLATE TO THE NECK OF THE ELECTRON LAMP
BR7907311A (en) PERFECT PROCESS FOR POLYMERIZATION OF BUTADIENE-1,3 TO PRODUCE CIS-1,4-POLYBUTADIENE FREE OF GEL AND MAINLY LINEAR
GB1546237A (en) Process for midifying proteins and the resulting proteins
CH416318A (en) Process for the production of diazotype copies according to the so-called semi-wet development method and transparent diazotype material for carrying out this process
BR7600989A (en) IMMOBILIZED ENZYME SET AND PROCESS FOR ITS PREPARATION
ATA223579A (en) METHOD FOR PRODUCING AN AGGREGATE OF BACTERIA CELLS
ATE13518T1 (en) PROCESS FOR ISOMERIZATION OF PARAFFINS.
ATE1954T1 (en) PROCESS FOR THE PREPARATION OF 19-HYDROXYSTEROIDS OF THE ANDROSTANE AND PREGNAN SERIES.
Anderson et al. RIVER: Recommendations for Improving the Valley Environmental Resources
ES246341A1 (en) A method of propagation of mammali cells (Machine-translation by Google Translate, not legally binding)
Neuhart Peroxidase, protein, and growth responses of cultured tomato pith explants
KR880010119A (en) Method for producing sikonin by culturing the cells of branch plants
SE7803732L (en) PROCEDURE AND MEANS OF PERFORMING AN ENZYMATIC PROCESS
Izadpanah Isolation and purification of actin and a 51 K dalton protein from haploid cells of physarum flavicomum
AU207572B2 (en) Improvements in or relating to polymerization process for making polymers acrylonitrile and alkenyl aromatic hydrocarbons
AU501154A (en) Method of producing dextran material by bacteriological and enzymatic action

Legal Events

Date Code Title Description
NAV Patent application has lapsed

Ref document number: 7902140-8

Format of ref document f/p: F