SE0702354L - Ways to detect previous ethanol intake - Google Patents
Ways to detect previous ethanol intakeInfo
- Publication number
- SE0702354L SE0702354L SE0702354A SE0702354A SE0702354L SE 0702354 L SE0702354 L SE 0702354L SE 0702354 A SE0702354 A SE 0702354A SE 0702354 A SE0702354 A SE 0702354A SE 0702354 L SE0702354 L SE 0702354L
- Authority
- SE
- Sweden
- Prior art keywords
- ethanol
- peth
- intake
- quantification
- ways
- Prior art date
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title abstract 12
- 238000004458 analytical method Methods 0.000 abstract 2
- 238000005251 capillar electrophoresis Methods 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000011002 quantification Methods 0.000 abstract 2
- 102000011420 Phospholipase D Human genes 0.000 abstract 1
- 108090000553 Phospholipase D Proteins 0.000 abstract 1
- JCABVIFDXFFRMT-DIPNUNPCSA-N [(2r)-1-[ethoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] octadec-9-enoate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OCC)OC(=O)CCCCCCCC=CCCCCCCCC JCABVIFDXFFRMT-DIPNUNPCSA-N 0.000 abstract 1
- 235000013334 alcoholic beverage Nutrition 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- ZJXZSIYSNXKHEA-UHFFFAOYSA-N ethyl dihydrogen phosphate Chemical compound CCOP(O)(O)=O ZJXZSIYSNXKHEA-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/98—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving alcohol, e.g. ethanol in breath
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/04—Phospholipids, i.e. phosphoglycerides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention comprises a method for assessing previous intake of ethanol, the main alcohol constituent in alcoholic beverages, from a biological sample. Level of ethanol intake is determined by measuring phosphatidylethanol (Peth), which is formed in cell membranes via the enzyme phospholipase D, only in the presence of ethanol. The present invention mainly relates to an analytical method based on capillary electrophoresis in which Peth is separated from other compounds, enabling detection and quantification. The invention further relates to an analytical method in which non aqueous capillary electrophoresis and stacking is combined, in order to enhance detection and quantification of Peth. The present invention also relates to a method for detecting and/or quantifying ethanol intake by determining a ratio of at least two ethanol metabolites, preferably Peth and Ethyl phosphate.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0702354A SE531588C2 (en) | 2007-10-23 | 2007-10-23 | Ways to detect previous ethanol intake |
| PCT/SE2008/051187 WO2009054784A1 (en) | 2007-10-23 | 2008-10-22 | Method for assessing previous ethanol intake |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0702354A SE531588C2 (en) | 2007-10-23 | 2007-10-23 | Ways to detect previous ethanol intake |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE0702354L true SE0702354L (en) | 2009-04-23 |
| SE531588C2 SE531588C2 (en) | 2009-06-02 |
Family
ID=40579769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE0702354A SE531588C2 (en) | 2007-10-23 | 2007-10-23 | Ways to detect previous ethanol intake |
Country Status (2)
| Country | Link |
|---|---|
| SE (1) | SE531588C2 (en) |
| WO (1) | WO2009054784A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8795980B2 (en) | 2010-07-09 | 2014-08-05 | Pethmark AB | Methods for determination of previous ethanol consumption |
| SE2050826A1 (en) | 2020-07-02 | 2022-01-03 | Capitainer Ab | Functionalized blood sampling device and method for peth measurement |
| WO2022003178A1 (en) | 2020-07-02 | 2022-01-06 | Capitainer Ab | Blood sampling device and method for peth measurement |
| CN113970603A (en) * | 2020-07-24 | 2022-01-25 | 复旦大学 | Biomarkers for distinguishing alcohol death from post-mortem alcohol filling |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060084134A1 (en) * | 2004-06-10 | 2006-04-20 | Wurst Friedrich M | Direct ethanol metabolite ethyl sulfate as an useful diagnostic and therapeutic marker of alcohol consumption |
-
2007
- 2007-10-23 SE SE0702354A patent/SE531588C2/en not_active IP Right Cessation
-
2008
- 2008-10-22 WO PCT/SE2008/051187 patent/WO2009054784A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009054784A1 (en) | 2009-04-30 |
| SE531588C2 (en) | 2009-06-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NUG | Patent has lapsed |