SE0203770D0 - Method of separation - Google Patents
Method of separationInfo
- Publication number
- SE0203770D0 SE0203770D0 SE0203770A SE0203770A SE0203770D0 SE 0203770 D0 SE0203770 D0 SE 0203770D0 SE 0203770 A SE0203770 A SE 0203770A SE 0203770 A SE0203770 A SE 0203770A SE 0203770 D0 SE0203770 D0 SE 0203770D0
- Authority
- SE
- Sweden
- Prior art keywords
- antithrombin
- latent
- native
- elution
- concentration
- Prior art date
Links
Abstract
A method for separation of native antithrombin and pre-latent antithrombin present in a sample from each other is provided. The method comprises performing, in the order given, the following steps: a) application of a sample containing native antithrombin and pre-latent antithrombin to an affinity material with affinity for antithrombin, wherein said application is performed under conditions such that binding of native antithrombin and pre-latent antithrombin to the affinity material is achieved; b) application of a solution of an elution salt S1 to said affinity material at a concentration C1 of elution salt, for elution of pre-latent antithrombin, said concentration C1 is in the range of 30-95 % of the concentration needed for elution of native antithrombin with the elution salt S1. In the method, the concentration C1 is such that pre-latent antithrombin is eluted and native antithrombin is retained.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0203770A SE0203770D0 (en) | 2002-12-19 | 2002-12-19 | Method of separation |
PCT/EP2003/014571 WO2004056870A2 (en) | 2002-12-19 | 2003-12-18 | Method for separation of antithrombin |
AU2003290096A AU2003290096A1 (en) | 2002-12-19 | 2003-12-18 | Method for separation of antithrombin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0203770A SE0203770D0 (en) | 2002-12-19 | 2002-12-19 | Method of separation |
Publications (1)
Publication Number | Publication Date |
---|---|
SE0203770D0 true SE0203770D0 (en) | 2002-12-19 |
Family
ID=20289922
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE0203770A SE0203770D0 (en) | 2002-12-19 | 2002-12-19 | Method of separation |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2003290096A1 (en) |
SE (1) | SE0203770D0 (en) |
WO (1) | WO2004056870A2 (en) |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE392038B (en) * | 1971-09-08 | 1977-03-14 | Kabi Ab | PROCEDURE FOR INSULATION OF ANTITROMBIN FROM BLOOD OR BLOOD PRODUCTS |
US4087415A (en) * | 1976-06-09 | 1978-05-02 | William L. Wilson | Antithrombin III |
JPS597694B2 (en) * | 1981-07-13 | 1984-02-20 | 日本赤十字社 | Purification method of antithrombin 3 using inactivated thrombin gel |
JP2729484B2 (en) * | 1988-04-28 | 1998-03-18 | 株式会社ミドリ十字 | Purification method of antithrombin-III |
WO2002038610A1 (en) * | 2000-11-08 | 2002-05-16 | Octapharma Ag | Process for the preparation of latent antithrombin iii |
WO2002060925A1 (en) * | 2001-01-30 | 2002-08-08 | Genzymes Corporation | Method of producing latent antithrombin iii |
-
2002
- 2002-12-19 SE SE0203770A patent/SE0203770D0/en unknown
-
2003
- 2003-12-18 AU AU2003290096A patent/AU2003290096A1/en not_active Abandoned
- 2003-12-18 WO PCT/EP2003/014571 patent/WO2004056870A2/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO2004056870A2 (en) | 2004-07-08 |
AU2003290096A8 (en) | 2004-07-14 |
WO2004056870A3 (en) | 2004-10-14 |
AU2003290096A1 (en) | 2004-07-14 |
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