SE0203770D0 - Method of separation - Google Patents

Method of separation

Info

Publication number
SE0203770D0
SE0203770D0 SE0203770A SE0203770A SE0203770D0 SE 0203770 D0 SE0203770 D0 SE 0203770D0 SE 0203770 A SE0203770 A SE 0203770A SE 0203770 A SE0203770 A SE 0203770A SE 0203770 D0 SE0203770 D0 SE 0203770D0
Authority
SE
Sweden
Prior art keywords
antithrombin
latent
native
elution
concentration
Prior art date
Application number
SE0203770A
Other languages
Swedish (sv)
Inventor
Goeran Karlsson
Stefan Winge
Original Assignee
Biovitrum Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biovitrum Ab filed Critical Biovitrum Ab
Priority to SE0203770A priority Critical patent/SE0203770D0/en
Publication of SE0203770D0 publication Critical patent/SE0203770D0/en
Priority to PCT/EP2003/014571 priority patent/WO2004056870A2/en
Priority to AU2003290096A priority patent/AU2003290096A1/en

Links

Abstract

A method for separation of native antithrombin and pre-latent antithrombin present in a sample from each other is provided. The method comprises performing, in the order given, the following steps: a) application of a sample containing native antithrombin and pre-latent antithrombin to an affinity material with affinity for antithrombin, wherein said application is performed under conditions such that binding of native antithrombin and pre-latent antithrombin to the affinity material is achieved; b) application of a solution of an elution salt S1 to said affinity material at a concentration C1 of elution salt, for elution of pre-latent antithrombin, said concentration C1 is in the range of 30-95 % of the concentration needed for elution of native antithrombin with the elution salt S1. In the method, the concentration C1 is such that pre-latent antithrombin is eluted and native antithrombin is retained.
SE0203770A 2002-12-19 2002-12-19 Method of separation SE0203770D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE0203770A SE0203770D0 (en) 2002-12-19 2002-12-19 Method of separation
PCT/EP2003/014571 WO2004056870A2 (en) 2002-12-19 2003-12-18 Method for separation of antithrombin
AU2003290096A AU2003290096A1 (en) 2002-12-19 2003-12-18 Method for separation of antithrombin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0203770A SE0203770D0 (en) 2002-12-19 2002-12-19 Method of separation

Publications (1)

Publication Number Publication Date
SE0203770D0 true SE0203770D0 (en) 2002-12-19

Family

ID=20289922

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0203770A SE0203770D0 (en) 2002-12-19 2002-12-19 Method of separation

Country Status (3)

Country Link
AU (1) AU2003290096A1 (en)
SE (1) SE0203770D0 (en)
WO (1) WO2004056870A2 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE392038B (en) * 1971-09-08 1977-03-14 Kabi Ab PROCEDURE FOR INSULATION OF ANTITROMBIN FROM BLOOD OR BLOOD PRODUCTS
US4087415A (en) * 1976-06-09 1978-05-02 William L. Wilson Antithrombin III
JPS597694B2 (en) * 1981-07-13 1984-02-20 日本赤十字社 Purification method of antithrombin 3 using inactivated thrombin gel
JP2729484B2 (en) * 1988-04-28 1998-03-18 株式会社ミドリ十字 Purification method of antithrombin-III
WO2002038610A1 (en) * 2000-11-08 2002-05-16 Octapharma Ag Process for the preparation of latent antithrombin iii
WO2002060925A1 (en) * 2001-01-30 2002-08-08 Genzymes Corporation Method of producing latent antithrombin iii

Also Published As

Publication number Publication date
WO2004056870A2 (en) 2004-07-08
AU2003290096A8 (en) 2004-07-14
WO2004056870A3 (en) 2004-10-14
AU2003290096A1 (en) 2004-07-14

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