RU99124840A - SYPHILIS DIAGNOSTIC METHOD - Google Patents

SYPHILIS DIAGNOSTIC METHOD

Info

Publication number
RU99124840A
RU99124840A RU99124840/14A RU99124840A RU99124840A RU 99124840 A RU99124840 A RU 99124840A RU 99124840/14 A RU99124840/14 A RU 99124840/14A RU 99124840 A RU99124840 A RU 99124840A RU 99124840 A RU99124840 A RU 99124840A
Authority
RU
Russia
Prior art keywords
solid
test serum
syphilis
carrier
phase
Prior art date
Application number
RU99124840/14A
Other languages
Russian (ru)
Other versions
RU2200327C2 (en
Inventor
Игорь Александрович Базиков
Ирина Степановна Тюменцева
Виталий Иванович Ефременко
Евгений Николаевич Афанасьев
Ирина Викторовна Жарникова
Original Assignee
Ставропольский научно-исследовательский противочумный институт
Filing date
Publication date
Application filed by Ставропольский научно-исследовательский противочумный институт filed Critical Ставропольский научно-исследовательский противочумный институт
Priority to RU99124840/14A priority Critical patent/RU2200327C2/en
Priority claimed from RU99124840/14A external-priority patent/RU2200327C2/en
Publication of RU99124840A publication Critical patent/RU99124840A/en
Application granted granted Critical
Publication of RU2200327C2 publication Critical patent/RU2200327C2/en

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Claims (1)

Способ диагностики сифилиса методами иммуноферментного анализа (ИФА) и количественного иммунофлюоресцентного анализа (КИФА), включающий сенсибилизацию твердофазного носителя ультраозвученным афинно-очищенным трепонемным антигеном, инкубацию его с исследуемой сывороткой, а затем с маркированным конъюгатом антивидовыми моноклональными иммуноглобулинами, нанесение в случае ИФА субстрат-индикаторного реагента и промежуточные отмывки носителя с последующим учетом результатов реакции, отличающийся тем, что в качестве твердофазного носителя используют модифицированные бензохиноном магнитные композиционные сорбенты на основе кремнезема и поэтапно переносят их в исследуемую сыворотку, конъюгирующий и субстратный реагенты и отмывающий раствор.A method for the diagnosis of syphilis by enzyme-linked immunosorbent assay (ELISA) and quantitative immunofluorescence assay (KIFA), comprising sensitizing a solid-phase carrier with an ultra-sonic affinity-purified treponemal antigen, incubating it with the test serum, and then with a conjugate labeled with antiviral monoclonal immunoglobulin conjugate reagent and intermediate washing of the carrier, followed by the results of the reaction, characterized in that as a solid-phase but The carriers use modified silica-based magnetic benzoquinone-modified sorbents and gradually transfer them to the test serum, conjugating and substrate reagents, and a washing solution.
RU99124840/14A 1999-11-25 1999-11-25 Method for diagnostics of syphilis (variants) RU2200327C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU99124840/14A RU2200327C2 (en) 1999-11-25 1999-11-25 Method for diagnostics of syphilis (variants)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU99124840/14A RU2200327C2 (en) 1999-11-25 1999-11-25 Method for diagnostics of syphilis (variants)

Publications (2)

Publication Number Publication Date
RU99124840A true RU99124840A (en) 2001-08-20
RU2200327C2 RU2200327C2 (en) 2003-03-10

Family

ID=20227380

Family Applications (1)

Application Number Title Priority Date Filing Date
RU99124840/14A RU2200327C2 (en) 1999-11-25 1999-11-25 Method for diagnostics of syphilis (variants)

Country Status (1)

Country Link
RU (1) RU2200327C2 (en)

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