RU97111288A - METHOD FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS IN PATIENTS WITH IMMUNO DEFICIENCY - Google Patents

METHOD FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS IN PATIENTS WITH IMMUNO DEFICIENCY

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Publication number
RU97111288A
RU97111288A RU97111288/14A RU97111288A RU97111288A RU 97111288 A RU97111288 A RU 97111288A RU 97111288/14 A RU97111288/14 A RU 97111288/14A RU 97111288 A RU97111288 A RU 97111288A RU 97111288 A RU97111288 A RU 97111288A
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RU
Russia
Prior art keywords
reaction
level
serum
extinction
antigen
Prior art date
Application number
RU97111288/14A
Other languages
Russian (ru)
Other versions
RU2133965C1 (en
Inventor
А.В. Баенский
А.В. Ватазин
Д.В. Перлин
Е.О. Щербаков
Е.И. Прокопенко
И.П. Иванова
Original Assignee
Московский областной научно-исследовательский клинический институт
Filing date
Publication date
Application filed by Московский областной научно-исследовательский клинический институт filed Critical Московский областной научно-исследовательский клинический институт
Priority to RU97111288A priority Critical patent/RU2133965C1/en
Priority claimed from RU97111288A external-priority patent/RU2133965C1/en
Publication of RU97111288A publication Critical patent/RU97111288A/en
Application granted granted Critical
Publication of RU2133965C1 publication Critical patent/RU2133965C1/en

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Claims (1)

Способ серологической диагностики туберкулеза у больных с иммунодефицитом, включающий в себя иммуноферментный анализ для определения уровня антител в контрольной и опытной сыворотках к микобактериальному антигену по уровню относительной экстинкции, на основании которой судят о положительности реакции, отличающийся тем, что после определения экстинкции контрольной сыворотки с микобактериальным антигеном, дополнительно ставят реакцию этой же сыворотки с общим перекрестнореагирующим бактериальным антигеном, и уровень экстинкции ее доводят до уровня реакции с микобактериальным антигеном, затем опытную сыворотку ставят в этом же разведении с общим бактериальным антигеном, измеряют величину относительной экстинкции опытной сыворотки, после чего определяют коэффициент, соответствующий разнице в относительной экстинкции между контрольной и опытной сыворотками при постановке реакции с общим бактериальным антигеном, и при постановке реакции опытной сыворотки с микобактериальным антигеном повышают уровень положительности реакции с учетом этого коэффициента.A method for the serological diagnosis of tuberculosis in patients with immunodeficiency, including an enzyme-linked immunosorbent assay to determine the level of antibodies in the control and experimental sera to mycobacterial antigen by the level of relative extinction, on the basis of which a positive reaction is judged, characterized in that after determining the extinction of the control serum with mycobacterial antigen, additionally put the reaction of the same serum with a common cross-reactive bacterial antigen, and its extinction level adjusted to the level of reaction with mycobacterial antigen, then the test serum is placed in the same dilution with the total bacterial antigen, the relative extinction value of the test serum is measured, and then the coefficient corresponding to the difference in relative extinction between the control and experimental serums is determined when the reaction is set up with the common bacterial antigen , and when setting the reaction of the experimental serum with mycobacterial antigen, the level of positive reaction is increased taking into account this coefficient.
RU97111288A 1997-07-08 1997-07-08 Serological method for diagnosing tuberculosis in patients suffering from immunodeficiency RU2133965C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU97111288A RU2133965C1 (en) 1997-07-08 1997-07-08 Serological method for diagnosing tuberculosis in patients suffering from immunodeficiency

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU97111288A RU2133965C1 (en) 1997-07-08 1997-07-08 Serological method for diagnosing tuberculosis in patients suffering from immunodeficiency

Publications (2)

Publication Number Publication Date
RU97111288A true RU97111288A (en) 1999-06-10
RU2133965C1 RU2133965C1 (en) 1999-07-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU97111288A RU2133965C1 (en) 1997-07-08 1997-07-08 Serological method for diagnosing tuberculosis in patients suffering from immunodeficiency

Country Status (1)

Country Link
RU (1) RU2133965C1 (en)

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