RU96116371A - METHOD FOR PRODUCING COMPLETE PLANTS AND TULIP MICROBROWS FROM ISOLATED GERM - Google Patents

METHOD FOR PRODUCING COMPLETE PLANTS AND TULIP MICROBROWS FROM ISOLATED GERM

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Publication number
RU96116371A
RU96116371A RU96116371/13A RU96116371A RU96116371A RU 96116371 A RU96116371 A RU 96116371A RU 96116371/13 A RU96116371/13 A RU 96116371/13A RU 96116371 A RU96116371 A RU 96116371A RU 96116371 A RU96116371 A RU 96116371A
Authority
RU
Russia
Prior art keywords
embryos
tulip
nutrient medium
medium
microbrows
Prior art date
Application number
RU96116371/13A
Other languages
Russian (ru)
Other versions
RU2123256C1 (en
Inventor
Т.М. Коломиец
В.С. Мохно
Original Assignee
Т.М. Коломиец
В.С. Мохно
Filing date
Publication date
Application filed by Т.М. Коломиец, В.С. Мохно filed Critical Т.М. Коломиец
Priority to RU96116371A priority Critical patent/RU2123256C1/en
Priority claimed from RU96116371A external-priority patent/RU2123256C1/en
Publication of RU96116371A publication Critical patent/RU96116371A/en
Application granted granted Critical
Publication of RU2123256C1 publication Critical patent/RU2123256C1/en

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Claims (1)

Способ получения полноценных растений и микролуковичек из изолированных зародышей тюльпанов, включающий введение зародышей в культуру, культивирование на питательной среде с последующим наращиванием вегетативной массы, перенос на питательную среду для образования дочерних луковичек, отличающийся тем, что с цепью увеличения жизнеспособности межвидовых и полиплоидных гибридов, а также значительного повышения коэффициента размножения, используют не полностью вызревшие зародыши, достигшие 3-5 мм, высаживают сначала на среду Ван Гофа, а через 2-3 недели на модифицированную среду Мурасиге и Скуга, с добавлением солей никеля и алюминия, регуляторов поста и увеличением количества сахарозы для образования вегетативной массы, затем с целью луковицеобразования питательную среду корректируют, исключая НУК и БАП и добавляя ИМК, при последовательной смене температурного и светового режимов.A method of obtaining full-fledged plants and microtubes from isolated tulip embryos, including the introduction of embryos into a culture, cultivation on a nutrient medium followed by growth of vegetative mass, transfer to a nutrient medium for the formation of daughter bulbs, characterized in that with a chain for increasing the viability of interspecific and polyploid hybrids, also a significant increase in the reproduction rate, using not fully ripened embryos, reaching 3-5 mm, are first planted on Van Gough's medium, and After 2-3 weeks on the modified Murashige and Skoog medium, with the addition of nickel and aluminum salts, fasting regulators and an increase in the amount of sucrose to form a vegetative mass, then the nutrient medium is adjusted for bulb formation, excluding NAA and BAP and adding IMC, with a successive change in temperature and light modes.
RU96116371A 1996-08-08 1996-08-08 Method of preparing tulip microbulbs from isolated embryos under in vitro conditions RU2123256C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU96116371A RU2123256C1 (en) 1996-08-08 1996-08-08 Method of preparing tulip microbulbs from isolated embryos under in vitro conditions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU96116371A RU2123256C1 (en) 1996-08-08 1996-08-08 Method of preparing tulip microbulbs from isolated embryos under in vitro conditions

Publications (2)

Publication Number Publication Date
RU96116371A true RU96116371A (en) 1998-11-20
RU2123256C1 RU2123256C1 (en) 1998-12-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU96116371A RU2123256C1 (en) 1996-08-08 1996-08-08 Method of preparing tulip microbulbs from isolated embryos under in vitro conditions

Country Status (1)

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RU (1) RU2123256C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD3899C2 (en) * 2009-02-23 2009-12-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for micropropagation of Echinacea purpurea L. Moench in vitro
MD31Z (en) * 2009-02-23 2010-01-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Process for micropropagation in vitro of Echinacea purpurea L. Moench

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