RU96115817A - METHOD FOR PRODUCING RECOMBINANT INTERFERON-ALPHA-2 FROM INSOLVED INCLUSION BODIES - Google Patents
METHOD FOR PRODUCING RECOMBINANT INTERFERON-ALPHA-2 FROM INSOLVED INCLUSION BODIESInfo
- Publication number
- RU96115817A RU96115817A RU96115817/13A RU96115817A RU96115817A RU 96115817 A RU96115817 A RU 96115817A RU 96115817/13 A RU96115817/13 A RU 96115817/13A RU 96115817 A RU96115817 A RU 96115817A RU 96115817 A RU96115817 A RU 96115817A
- Authority
- RU
- Russia
- Prior art keywords
- inclusion bodies
- alpha
- producing recombinant
- recombinant interferon
- insolved
- Prior art date
Links
- 210000003000 Inclusion Bodies Anatomy 0.000 title claims 4
- 102100008763 IFNA2 Human genes 0.000 title claims 2
- 108010078049 Interferon alpha-2 Proteins 0.000 title claims 2
- 238000004519 manufacturing process Methods 0.000 title 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 239000004202 carbamide Substances 0.000 claims 2
- 239000012535 impurity Substances 0.000 claims 2
- 238000004153 renaturation Methods 0.000 claims 2
- 238000005063 solubilization Methods 0.000 claims 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N Glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 1
- 229960003180 Glutathione Drugs 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 108010050904 Interferons Proteins 0.000 claims 1
- 102000014150 Interferons Human genes 0.000 claims 1
- 210000004027 cells Anatomy 0.000 claims 1
- 238000011097 chromatography purification Methods 0.000 claims 1
- 239000003599 detergent Substances 0.000 claims 1
- 238000000502 dialysis Methods 0.000 claims 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims 1
- 229940079322 interferon Drugs 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000001590 oxidative Effects 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU96115817A RU2123010C1 (en) | 1996-07-31 | 1996-07-31 | Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU96115817A RU2123010C1 (en) | 1996-07-31 | 1996-07-31 | Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2123010C1 RU2123010C1 (en) | 1998-12-10 |
RU96115817A true RU96115817A (en) | 1999-01-10 |
Family
ID=20184091
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU96115817A RU2123010C1 (en) | 1996-07-31 | 1996-07-31 | Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2123010C1 (en) |
-
1996
- 1996-07-31 RU RU96115817A patent/RU2123010C1/en active
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