RU96115817A - METHOD FOR PRODUCING RECOMBINANT INTERFERON-ALPHA-2 FROM INSOLVED INCLUSION BODIES - Google Patents

METHOD FOR PRODUCING RECOMBINANT INTERFERON-ALPHA-2 FROM INSOLVED INCLUSION BODIES

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Publication number
RU96115817A
RU96115817A RU96115817/13A RU96115817A RU96115817A RU 96115817 A RU96115817 A RU 96115817A RU 96115817/13 A RU96115817/13 A RU 96115817/13A RU 96115817 A RU96115817 A RU 96115817A RU 96115817 A RU96115817 A RU 96115817A
Authority
RU
Russia
Prior art keywords
inclusion bodies
alpha
producing recombinant
recombinant interferon
insolved
Prior art date
Application number
RU96115817/13A
Other languages
Russian (ru)
Other versions
RU2123010C1 (en
Inventor
Н.В. Бажутина
В.П. Гурьев
Т.Л. Гурьева
А.И. Закабунин
А.А. Колокольцов
М.А. Ковригина
О.А. Моисеенкова
В.П. Романов
И.В. Юдина
Original Assignee
Государственный научный центр вирусологии и биотехнологии "Вектор"
Filing date
Publication date
Application filed by Государственный научный центр вирусологии и биотехнологии "Вектор" filed Critical Государственный научный центр вирусологии и биотехнологии "Вектор"
Priority to RU96115817A priority Critical patent/RU2123010C1/en
Priority claimed from RU96115817A external-priority patent/RU2123010C1/en
Application granted granted Critical
Publication of RU2123010C1 publication Critical patent/RU2123010C1/en
Publication of RU96115817A publication Critical patent/RU96115817A/en

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Claims (1)

Способ получения рекомбинантного интерферона-альфа-2 из нерастворимых тел включения, включающий разрушение клеток ультразвуком, удаление примесных белков промыванием осадка неионными детергентами, солюбилизацию тел включения, ренатурацию полученного продукта и последующую хроматографическую очистку нативного белка, отличающийся тем, что солюбилизацию тел включения и разрушение олигомеров интерферона проводят реакцией окислительного сульфитолиза при концентрации белка 5-10 мг/мл в растворе 6-7 M Gdn-HCl, низкомолекулярные примеси отделяют диализом против 7-8 M мочевины, ренатурацию сульфитированного производного проводят в присутствии 3-4 M мочевины, используя смесь окисленного и восстановленного глутатиона в соотношении 1:10.A method of producing recombinant interferon-alpha-2 from insoluble inclusion bodies, including destruction of cells by ultrasound, removal of impurity proteins by washing the precipitate with nonionic detergents, solubilization of inclusion bodies, renaturation of the obtained product and subsequent chromatographic purification of the native protein, characterized in that the solubilization of inclusion bodies and the destruction of oligomers interferon is carried out by the reaction of oxidative sulfitolysis at a protein concentration of 5-10 mg / ml in a solution of 6-7 M Gdn-HCl, low molecular weight impurities t dialysis against 7-8 M urea, renaturation sulfited derivative is carried out in the presence of 3-4 M urea, using a mixture of oxidized and reduced glutathione at a ratio of 1:10.
RU96115817A 1996-07-31 1996-07-31 Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies RU2123010C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU96115817A RU2123010C1 (en) 1996-07-31 1996-07-31 Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU96115817A RU2123010C1 (en) 1996-07-31 1996-07-31 Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies

Publications (2)

Publication Number Publication Date
RU2123010C1 RU2123010C1 (en) 1998-12-10
RU96115817A true RU96115817A (en) 1999-01-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU96115817A RU2123010C1 (en) 1996-07-31 1996-07-31 Method of preparing recombinant interferon-alpha-2 from insoluble inclusion bodies

Country Status (1)

Country Link
RU (1) RU2123010C1 (en)

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