RU96112855A - METHOD FOR DETERMINING THE NUMBER OF CHICKEN PEPPSIN IN MIXTURE COMPOSITIONS WITH BEEF PEPPSIN - Google Patents
METHOD FOR DETERMINING THE NUMBER OF CHICKEN PEPPSIN IN MIXTURE COMPOSITIONS WITH BEEF PEPPSINInfo
- Publication number
- RU96112855A RU96112855A RU96112855/13A RU96112855A RU96112855A RU 96112855 A RU96112855 A RU 96112855A RU 96112855/13 A RU96112855/13 A RU 96112855/13A RU 96112855 A RU96112855 A RU 96112855A RU 96112855 A RU96112855 A RU 96112855A
- Authority
- RU
- Russia
- Prior art keywords
- peppsin
- chicken
- milk
- determining
- pepsin
- Prior art date
Links
- 235000015278 beef Nutrition 0.000 title claims 3
- 239000000203 mixture Substances 0.000 title 2
- 108090000284 Pepsin A Proteins 0.000 claims 6
- 229940111202 pepsin Drugs 0.000 claims 6
- 241000287828 Gallus gallus Species 0.000 claims 4
- 210000004080 Milk Anatomy 0.000 claims 4
- 239000007853 buffer solution Substances 0.000 claims 4
- 239000003814 drug Substances 0.000 claims 4
- 229940079593 drugs Drugs 0.000 claims 4
- 239000008267 milk Substances 0.000 claims 4
- 235000013336 milk Nutrition 0.000 claims 4
- 230000015271 coagulation Effects 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- 238000005345 coagulation Methods 0.000 claims 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 206010009802 Coagulopathy Diseases 0.000 claims 1
- ZSJHIZJESFFXAU-UHFFFAOYSA-N boric acid;phosphoric acid Chemical compound OB(O)O.OP(O)(O)=O ZSJHIZJESFFXAU-UHFFFAOYSA-N 0.000 claims 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 1
- 230000035602 clotting Effects 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
Claims (1)
где X - содержание куриного пепсина в исследуемом препарате, %;
T1 - продолжительность свертывания молока буферным раствором препарата, не подвергнутого тепловой обработке, мин;
T2 - продолжительность свертывания молока буферным раствором препарата, выдержанного 3 мин при 46oC, мин.A method for determining the amount of chicken pepsin in milk-clotting preparations, comprising inhibiting beef pepsin, adding a buffer solution of the drug to milk and setting the duration of milk clotting, followed by determining the amount of chicken pepsin in a calculation way, characterized in that a buffer solution of the test preparation not subjected to heat is used as a control processing, and inhibition of beef pepsin is carried out by extracting a 2% solution of the studied drug and for 3 min at 46 o C in 0,076 mol / liter of phosphate-borate buffer at pH 7.6, while the content of chicken pepsin is determined by the formula
where X is the content of chicken pepsin in the test drug,%;
T 1 - the duration of coagulation of milk with a buffer solution of the drug, not subjected to heat treatment, min;
T 2 - the duration of coagulation of milk with a buffer solution of the preparation, aged 3 min at 46 o C, min
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU96112855A true RU96112855A (en) | 1998-11-27 |
Family
ID=
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