RU96105572A - Method of stabilizing olynonucleotide duplexes - Google Patents
Method of stabilizing olynonucleotide duplexesInfo
- Publication number
- RU96105572A RU96105572A RU96105572/13A RU96105572A RU96105572A RU 96105572 A RU96105572 A RU 96105572A RU 96105572/13 A RU96105572/13 A RU 96105572/13A RU 96105572 A RU96105572 A RU 96105572A RU 96105572 A RU96105572 A RU 96105572A
- Authority
- RU
- Russia
- Prior art keywords
- radical
- aminoalkoxy
- oligodeoxyribonucleotides
- aminoalkyl
- radicals
- Prior art date
Links
- 230000000087 stabilizing Effects 0.000 title 1
- -1 aryloxide Chemical group 0.000 claims 5
- 229920000272 Oligonucleotide Polymers 0.000 claims 4
- 239000003446 ligand Substances 0.000 claims 4
- 230000027455 binding Effects 0.000 claims 3
- 125000002431 aminoalkoxy group Chemical group 0.000 claims 2
- 125000004103 aminoalkyl group Chemical group 0.000 claims 2
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 2
- CFCUWKMKBJTWLW-BGLFSJPPSA-N (2S,3S)-2-[(2S,4R,5R,6R)-4-[(2S,4R,5R,6R)-4-[(2S,4S,5R,6R)-4,5-dihydroxy-4,6-dimethyloxan-2-yl]oxy-5-hydroxy-6-methyloxan-2-yl]oxy-5-hydroxy-6-methyloxan-2-yl]oxy-3-[(1S,3S,4R)-3,4-dihydroxy-1-methoxy-2-oxopentyl]-6-[(2S,4R,5S,6R)-4-[(2S,4R,5S,6R)-4,5-dih Chemical compound O([C@@H]1C[C@@H](O[C@H](C)[C@@H]1O)OC=1C=C2C=C3C[C@H]([C@@H](C(=O)C3=C(O)C2=C(O)C=1C)O[C@@H]1O[C@H](C)[C@@H](O)[C@H](O[C@@H]2O[C@H](C)[C@@H](O)[C@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@@](C)(O)C3)C2)C1)[C@H](OC)C(=O)[C@@H](O)[C@@H](C)O)[C@H]1C[C@@H](O)[C@H](O)[C@@H](C)O1 CFCUWKMKBJTWLW-BGLFSJPPSA-N 0.000 claims 1
- QUHGSDZVAPFNLV-UHFFFAOYSA-N 4-[(5-acetamidofuran-2-carbonyl)amino]-N-[3-(dimethylamino)propyl]-1-propylpyrrole-2-carboxamide Chemical compound C1=C(C(=O)NCCCN(C)C)N(CCC)C=C1NC(=O)C1=CC=C(NC(C)=O)O1 QUHGSDZVAPFNLV-UHFFFAOYSA-N 0.000 claims 1
- MMURVNDSFNJHAM-OWOJBTEDSA-N 4-[(E)-2-(4-carbamimidoylphenyl)ethenyl]benzenecarboximidamide Chemical compound C1=CC(C(=N)N)=CC=C1\C=C\C1=CC=C(C(N)=N)C=C1 MMURVNDSFNJHAM-OWOJBTEDSA-N 0.000 claims 1
- STQGQHZAVUOBTE-VGBVRHCVSA-N DAUNOMYCIN Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 claims 1
- UPBAOYRENQEPJO-UHFFFAOYSA-N N-[5-[[5-[(3-amino-3-iminopropyl)carbamoyl]-1-methylpyrrol-3-yl]carbamoyl]-1-methylpyrrol-3-yl]-4-formamido-1-methylpyrrole-2-carboxamide Chemical compound CN1C=C(NC=O)C=C1C(=O)NC1=CN(C)C(C(=O)NC2=CN(C)C(C(=O)NCCC(N)=N)=C2)=C1 UPBAOYRENQEPJO-UHFFFAOYSA-N 0.000 claims 1
- IDBIFFKSXLYUOT-UHFFFAOYSA-N Netropsin Chemical compound C1=C(C(=O)NCCC(N)=N)N(C)C=C1NC(=O)C1=CC(NC(=O)CN=C(N)N)=CN1C IDBIFFKSXLYUOT-UHFFFAOYSA-N 0.000 claims 1
- 108010042309 Netropsin Proteins 0.000 claims 1
- 229960003171 Plicamycin Drugs 0.000 claims 1
- ZYVSOIYQKUDENJ-MNGCYDFMSA-N [6-[[(6S,7S)-6-[4-[4-(5-acetyloxy-4-hydroxy-4,6-dimethyloxan-2-yl)oxy-5-hydroxy-6-methyloxan-2-yl]oxy-5-hydroxy-6-methyloxan-2-yl]oxy-7-[(1S,3S,4R)-3,4-dihydroxy-1-methoxy-2-oxopentyl]-4,10-dihydroxy-3-methyl-5-oxo-7,8-dihydro-6H-anthracen-2-yl]oxy]-4-(4- Chemical compound C([C@H]([C@@H](C(=O)C1=C(O)C2=C(O)C=3C)OC4OC(C)C(O)C(OC5OC(C)C(O)C(OC6OC(C)C(OC(C)=O)C(C)(O)C6)C5)C4)[C@H](OC)C(=O)[C@@H](O)[C@@H](C)O)C1=CC2=CC=3OC(OC(C)C1OC(C)=O)CC1OC1CC(O)C(OC)C(C)O1 ZYVSOIYQKUDENJ-MNGCYDFMSA-N 0.000 claims 1
- 125000003545 alkoxy group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000000875 corresponding Effects 0.000 claims 1
- 125000004435 hydrogen atoms Chemical group [H]* 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical compound C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 claims 1
- 238000011105 stabilization Methods 0.000 claims 1
- 108010042747 stallimycin Proteins 0.000 claims 1
- 125000004001 thioalkyl group Chemical group 0.000 claims 1
Claims (1)
где радикалы В - природные или модифицированные основания нуклеиновых кислот;
радикалы Х - оксаанион 0-, тиоанион S-, алкильная группа, алкоксильная группа, арилоксидная группа, аминоалкильная группа, аминоалкоксильная группа, тиоалкильная группа или группировка -Y-Z;
радикалы R и R' могут быть идентичными и отличающимися и являются каждый атомом водорода или группировкой -Y-Z;
радикал Y - полиметиленовое звено или аминоалкильный, аминоалкоксильный, тиоалкильный радикал;
радикал Z - радикал, соответствующий малобороздочному лиганду, в качестве малобороздочных лигандов могут быть производные нетропсина, дистамицина, дауномицина, хромомицина, митрамицина, беренила, стильбамидина, СС-1065, Хехст 33258, SN6999, лекситропсины, содержащие имидазольные, пиррольные и тиазольные звенья;
n - число нуклеотидных звеньев в олигонуклеотиде.The method of stabilization of oligonucleotide duplexes containing conjugates of oligodeoxyribonucleotides with compounds that increase the stability of DNA duplex, characterized in that oligodeoxyribonucleotides covalently linked to ligands that bind in the minor groove of DNA are used as conjugates, the general formula
where radicals B are natural or modified nucleic acid bases;
X is oxa-anion 0 - , thioanion S - , alkyl, alkoxy, aryloxide, aminoalkyl, aminoalkoxy, thioalkyl, or -YZ;
the radicals R and R ′ may be identical and different and are each a hydrogen atom or a —YZ moiety;
the radical Y is a polymethylene unit or an aminoalkyl, aminoalkoxy, thioalkyl radical;
radical Z - a radical corresponding to the friable ligand, as short-ligand ligands can be derived from netropsin, distamycin, daunomycin, chromomycin, mitramycin, berenyl, stilbamidine, CC-1065, Hoechst 33258, SN6999, lexitropsin, containing idiamine, Iditraxamine, CC-1065;
n is the number of nucleotide units in the oligonucleotide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU96105572/13A RU2124523C1 (en) | 1996-03-22 | 1996-03-22 | Method of oligonucleotide duplex stabilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU96105572/13A RU2124523C1 (en) | 1996-03-22 | 1996-03-22 | Method of oligonucleotide duplex stabilization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU96105572A true RU96105572A (en) | 1998-06-27 |
| RU2124523C1 RU2124523C1 (en) | 1999-01-10 |
Family
ID=20178395
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU96105572/13A RU2124523C1 (en) | 1996-03-22 | 1996-03-22 | Method of oligonucleotide duplex stabilization |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2124523C1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2577534A4 (en) | 2010-05-27 | 2017-04-26 | Emerald Therapeutics, Inc. | System and method for propagating information using modified nucleic acids |
-
1996
- 1996-03-22 RU RU96105572/13A patent/RU2124523C1/en not_active IP Right Cessation
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