RU95117445A - METHOD FOR OBTAINING A LIVER INFLUENZA VACCINE - Google Patents

METHOD FOR OBTAINING A LIVER INFLUENZA VACCINE

Info

Publication number
RU95117445A
RU95117445A RU95117445/14A RU95117445A RU95117445A RU 95117445 A RU95117445 A RU 95117445A RU 95117445/14 A RU95117445/14 A RU 95117445/14A RU 95117445 A RU95117445 A RU 95117445A RU 95117445 A RU95117445 A RU 95117445A
Authority
RU
Russia
Prior art keywords
virus
influenza vaccine
liver
obtaining
lyophilization
Prior art date
Application number
RU95117445/14A
Other languages
Russian (ru)
Other versions
RU2080124C1 (en
Inventor
Э.Г. Лукьянова
М.Л. Филиппова
Б.А. Говердовский
Я.Р. Гускин
Original Assignee
Санкт-Петербургский научно-исследовательский институт вакцин и сывороток
Filing date
Publication date
Application filed by Санкт-Петербургский научно-исследовательский институт вакцин и сывороток filed Critical Санкт-Петербургский научно-исследовательский институт вакцин и сывороток
Priority to RU95117445A priority Critical patent/RU2080124C1/en
Priority claimed from RU95117445A external-priority patent/RU2080124C1/en
Application granted granted Critical
Publication of RU2080124C1 publication Critical patent/RU2080124C1/en
Publication of RU95117445A publication Critical patent/RU95117445A/en

Links

Claims (1)

Способ получения живой гриппозной вакцины, включающий культивирование вируса гриппа в развивающихся куриных эмбрионах, отделение вируссодержащей аллантоисной жидкости, осветление, очистку ультрацентрифугированием в градиенте плотности сахарозы, разведение вирусного концентрата фосфатным физиологическим буферным раствором, стабилизацию, стерилизующее фильтрование и лиофилизацию в присутствии сорбитоло-желатинольного протектора, отличающийся тем, что осветление вируссодержащей аллантоисной жидкости проводят непосредственно по мере ее отделения, разведение вирусного концентрата производят до значения обратного титра гемагглютинирующей активности 3200 - 6400, а протектор лиофилизации вносят перед стерилизующим фильтрованием.A method for producing a live influenza vaccine, including culturing the influenza virus in developing chicken embryos, separating the virus-containing allantoic fluid, clarifying, purifying by ultracentrifugation in the sucrose density gradient, diluting the virus concentrate with phosphate physiological buffer solution, stabilization, sterilizing filtration and lyophilization in the presence of a sorbitol-gelatin protector, characterized in that the clarification of the virus-containing allantoic fluid is carried out directly by as it is separated, the virus concentrate is diluted to a value of the reverse titer of hemagglutinating activity of 3200 - 6400, and the lyophilization protector is introduced before sterilizing filtration.
RU95117445A 1995-10-19 1995-10-19 Method of live antiinfluenza vaccine preparing RU2080124C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU95117445A RU2080124C1 (en) 1995-10-19 1995-10-19 Method of live antiinfluenza vaccine preparing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU95117445A RU2080124C1 (en) 1995-10-19 1995-10-19 Method of live antiinfluenza vaccine preparing

Publications (2)

Publication Number Publication Date
RU2080124C1 RU2080124C1 (en) 1997-05-27
RU95117445A true RU95117445A (en) 1997-07-27

Family

ID=20172823

Family Applications (1)

Application Number Title Priority Date Filing Date
RU95117445A RU2080124C1 (en) 1995-10-19 1995-10-19 Method of live antiinfluenza vaccine preparing

Country Status (1)

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RU (1) RU2080124C1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104694489A (en) * 2007-05-04 2015-06-10 巴克斯特国际公司 Formulation of sugar solutions for continuous ultracentrifugation for virus purification
FR2921387B1 (en) * 2007-09-26 2012-04-20 Sanofi Pasteur PROCESS FOR PRODUCING INFLUENZA VIRUS
RU2015103990A (en) 2008-09-24 2015-10-27 Медиммун, Ллк METHODS OF CULTIVATION OF CELLS, REPRODUCTION AND CLEANING OF VIRUSES
EA021095B1 (en) * 2011-06-22 2015-04-30 Республиканское Государственное Предприятие На Праве Хозяйственного Ведения "Научно-Исследовательский Институт Проблем Биологической Безопасности" Комитета Науки Министерства Образования И Науки Process for preparing inactivated intact virion hydroxide aluminium vaccine against a/h1n1 influenza
RU2493872C1 (en) * 2012-08-08 2013-09-27 Федеральное государственное унитарное предприятие "Научно-производственное объединение по медицинским иммунобиологическим препаратам "Микроген" Министерства здравоохранения Российской Федерации ( ФГУП "НПО "Микроген" Минздрава России) Method for influenza virus purification
RU2535153C1 (en) * 2013-09-04 2014-12-10 Федеральное государственное унитарное предприятие "Санкт-Петербургский научно-исследовательский институт вакцин и сывороток и предприятие по производству бактерийных препаратов" Федерального медико-биологического агентства России (ФГУП СПбНИИВС ФМБА России) Method for preparing high-purity virion concentrate
CN106163554B (en) * 2014-12-23 2021-09-07 依生生物制药(新加坡)私人有限公司 Rabies composition containing PIKA adjuvant

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