RU95106532A - Method for carrying out express diagnosis of congenital infections
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Method for carrying out express diagnosis of congenital infections
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Publication number
RU95106532A
RU95106532ARU95106532/14ARU95106532ARU95106532ARU 95106532 ARU95106532 ARU 95106532ARU 95106532/14 ARU95106532/14 ARU 95106532/14ARU 95106532 ARU95106532 ARU 95106532ARU 95106532 ARU95106532 ARU 95106532A
Научно-исследовательский институт детских инфекций
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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
FIELD: medicine. SUBSTANCE: method involves determining antibodies and antigens in blood serum based on catalase activity lysed sheep erythrocytes, removing proper complement by its sorption for neutral complex of hemolysine and sheep erythrocytes, treated with 7.5-15 % hydrogen peroxide solution. Numeric value determined from enzyme reaction is used for determining antibodies or antigen in the material under test during 3-4 h. EFFECT: simplified method.
Claims (1)
Способ относится к медицине, в частности к экспресс-диагностике врожденных вирусных инфекций у детей. Целью способа является его упрощение с возможностью использования коммерческих диагностикумов для индикации тел к герпесу, цитомегалии, краснухи и токсоплазмозу. Предлагается определение антител или антигена в РСК по каталазной активности лизированных бараньих эритроцитов. Предлагается также удаление собственного комплемента с помощью сорбции его на нейтральный комплекс гемолизина и бараньих эритроцитов, обработанных 7,5 - 15%-ной перекисью водорода. Способ позволяет в течение 3 - 4 ч определить антитела или антиген в обследуемом материале количественно по цифровому показателю ферментной реакции.The method relates to medicine, in particular to the rapid diagnosis of congenital viral infections in children. The aim of the method is to simplify it with the possibility of using commercial diagnostics to indicate bodies for herpes, cytomegaly, rubella and toxoplasmosis. The definition of antibodies or antigen in CSC by the catalase activity of lysed sheep red blood cells is proposed. It is also proposed to remove one's own complement by sorbing it onto a neutral complex of hemolysin and mutton erythrocytes treated with 7.5-15% hydrogen peroxide. The method allows for 3 to 4 hours to determine the antibodies or antigen in the test material quantitatively by the digital index of the enzyme reaction.
RU95106532A1995-04-241995-04-24Method of express-diagnosis of congenital infections in children
RU2089910C1
(en)
Protein L and subfragments thereof, with immunoglobulin binding activity, a process for preparing thereof, reagent kit, pharmaceutical composition and a peptococcus magnus strain
Agglutination type immunological determn. of immunoglobulin antibodies - by adsorbing an anti-human IG on a solid, incubating with the sample and treating the adsorbed fraction with insoluble antigen