RU95104437A - Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine - Google Patents

Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine

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Publication number
RU95104437A
RU95104437A RU95104437/13A RU95104437A RU95104437A RU 95104437 A RU95104437 A RU 95104437A RU 95104437/13 A RU95104437/13 A RU 95104437/13A RU 95104437 A RU95104437 A RU 95104437A RU 95104437 A RU95104437 A RU 95104437A
Authority
RU
Russia
Prior art keywords
thymidine
strain
bacillus amyloliquefaciens
synthesis
producer
Prior art date
Application number
RU95104437/13A
Other languages
Russian (ru)
Other versions
RU2088659C1 (en
Inventor
Н.В. Королькова
А.С. Миронов
А.Г. Гарибян
Б.М. Полануер
Б.В. Тяглов
Original Assignee
Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов filed Critical Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов
Priority to RU9595104437A priority Critical patent/RU2088659C1/en
Publication of RU95104437A publication Critical patent/RU95104437A/en
Application granted granted Critical
Publication of RU2088659C1 publication Critical patent/RU2088659C1/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

FIELD: biotechnology, microbiology. SUBSTANCE: the proposed strain Bacillus amyloliquefaciens "КГМ"-5 produces thymidine (up to 4.5 g/l) for 96 h fermentation on medium containing, %: glucose 12; food yeast - paprin 3.4; ammonium nitrate 2.5; magnesium chloride 0.5, and chalk 2. Strain exhibits resistance to 5-fluorouracil, 5-fluorouridine, 5-fluorodeoxyuridine and auxotrophic properties with regard to adenine, histidine, thiamine and partial auxototrophic ones to tyrosine and asparagine. Synthesized thymidine is used as the parent compound for synthesis of azidothymidine - drug against AIDS. EFFECT: enhanced effectiveness of thymidine production.

Claims (1)

Изобретение относится к биотехнологии и микробиологической промышленности и касается прямого микробиологического синтеза тимидина, используемого в качестве исходного соединения для синтеза азидотимидина - лекарственного препарата против СПИДа. Предлагается штамм Bacillus amyloliquefaciens КГМ-5, продуцирующий до 4,5 г/л тимидина за 96 ч ферментации на среде, содержащей глюкозу (12%), дрожжи кормовые-паприн (3,4%), аммоний азотнокислый (2,5%), магний хлористый (0,5%), мел (2%). Штамм устойчив к 5-фторилацилу, 5-фторуридину, 5-фтордезоксиуридину и является ауксотрофом по аденину, гистидину, тиамину и неполным ауксотрофом по тирозину и аспарагину.The invention relates to biotechnology and the microbiological industry, and relates to the direct microbiological synthesis of thymidine, used as a starting compound for the synthesis of azidothymidine, a drug against AIDS. A strain of Bacillus amyloliquefaciens KGM-5 is proposed, producing up to 4.5 g / l thymidine in 96 hours of fermentation on a medium containing glucose (12%), fodder-paprine yeast (3.4%), ammonium nitrate (2.5%) , magnesium chloride (0.5%), chalk (2%). The strain is resistant to 5-fluorylacyl, 5-fluoruridine, 5-fluorodeoxyuridine and is an auxotroph for adenine, histidine, thiamine and an incomplete auxotroph for tyrosine and asparagine.
RU9595104437A 1995-03-27 1995-03-27 Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine RU2088659C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU9595104437A RU2088659C1 (en) 1995-03-27 1995-03-27 Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU9595104437A RU2088659C1 (en) 1995-03-27 1995-03-27 Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine

Publications (2)

Publication Number Publication Date
RU95104437A true RU95104437A (en) 1996-12-10
RU2088659C1 RU2088659C1 (en) 1997-08-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU9595104437A RU2088659C1 (en) 1995-03-27 1995-03-27 Strain of bacterium bacillus amyloliquefaciens - a producer of thymidine

Country Status (1)

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RU (1) RU2088659C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408667A (en) * 2019-06-29 2019-11-05 赤峰蒙广生物科技有限公司 A kind of zymotechnique improving beta-thymidine yield

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2509148C1 (en) * 2012-08-31 2014-03-10 Общество с ограниченной ответственностью "НОВА" Bacillus amyloliquefaciens BKM B-2714D STRAIN HAVING APPARENT ANTAGONISM IN RELATION TO Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes AND RESISTANCE TO TETRACYCLINE AND TRIMETHOPRIM

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408667A (en) * 2019-06-29 2019-11-05 赤峰蒙广生物科技有限公司 A kind of zymotechnique improving beta-thymidine yield

Also Published As

Publication number Publication date
RU2088659C1 (en) 1997-08-27

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