RU94021122A - Method of preparing interferon showing prolonged action - Google Patents

Method of preparing interferon showing prolonged action

Info

Publication number
RU94021122A
RU94021122A RU94021122/14A RU94021122A RU94021122A RU 94021122 A RU94021122 A RU 94021122A RU 94021122/14 A RU94021122/14 A RU 94021122/14A RU 94021122 A RU94021122 A RU 94021122A RU 94021122 A RU94021122 A RU 94021122A
Authority
RU
Russia
Prior art keywords
prolonged action
linking agent
mixture
cross
interferon
Prior art date
Application number
RU94021122/14A
Other languages
Russian (ru)
Inventor
Л.И. Стекольников
И.И. Самойленко
Original Assignee
Л.И. Стекольников
И.И. Самойленко
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Л.И. Стекольников, И.И. Самойленко filed Critical Л.И. Стекольников
Priority to RU94021122/14A priority Critical patent/RU94021122A/en
Publication of RU94021122A publication Critical patent/RU94021122A/en

Links

Abstract

FIELD: biotechnology. SUBSTANCE: method involves dissolving human leukocyte interferon in water, addition of polymer and cross-linking agent to solution, mixture incubation at 20-25 C and the end product drying. Used polymer is hyaluronic acid and cross-linking agent is diethylenetriaminepentaacetic acid calcium-trisodium salt (pentacin) that were taken at amount 10-20 mg and 2-4 mg, respectively, per 1000000 U human leukocyte interferon. Incubation of mixture is carried out for 0.5-1 h. Obtained preparation shows the expressed prolonged action (24 h, not less). EFFECT: improved method of preparing.

Claims (1)

Изобретение относится к области биотехнологии, а именно к способу получения интерферона с пролонгированным действием, который может быть использован в медицинской практике. Задача изобретения - получение человеческого лейкоцитарного интерферона (ЧЛИ) с выраженным пролонгированным действием (не менее 24 часов). Указанный результат достигается тем, что в способе получения ЧЛИ с пролонгированным действием, включающем растворение ЧЛИ в воде, добавление к раствору полимерного соединения и сшивающего агента, инкубацию смеси при 20 - 25°С и высушивание целевого продукта, в качестве полимерного соединения используют гиалуроновую кислоту, а в качестве сшивающего агента - кальций-тринатриевую соль диэтилентриаминопентауксусную кислоту (пентацин), при этом на 1000000 ЕД ЧЛИ берут 10 - 20 мг гиалуроновой кислоты и 2 - 4 мг пентацина, а инкубацию смеси осуществляют в течение 0,5 - 1 ч.The invention relates to the field of biotechnology, and in particular to a method for producing interferon with a prolonged action, which can be used in medical practice. The objective of the invention is the production of human leukocyte interferon (CHLI) with a pronounced prolonged action (at least 24 hours). The specified result is achieved by the fact that in the method for producing PLHI with prolonged action, including dissolving PLHI in water, adding a polymer compound and a cross-linking agent to the solution, incubating the mixture at 20 - 25 ° C and drying the target product, hyaluronic acid is used as the polymer compound, and as a cross-linking agent, calcium-trisodium salt of diethylenetriaminopentaacetic acid (pentacin), with 10 to 20 mg of hyaluronic acid and 2 to 4 mg of pentacin taken per 1,000,000 PIECES, and the mixture is incubated in 0.5 to 1 hour
RU94021122/14A 1994-06-09 1994-06-09 Method of preparing interferon showing prolonged action RU94021122A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94021122/14A RU94021122A (en) 1994-06-09 1994-06-09 Method of preparing interferon showing prolonged action

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94021122/14A RU94021122A (en) 1994-06-09 1994-06-09 Method of preparing interferon showing prolonged action

Publications (1)

Publication Number Publication Date
RU94021122A true RU94021122A (en) 1996-11-10

Family

ID=48447838

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94021122/14A RU94021122A (en) 1994-06-09 1994-06-09 Method of preparing interferon showing prolonged action

Country Status (1)

Country Link
RU (1) RU94021122A (en)

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