RU94020434A - Method and set for rift valley virus detection and antibodies to its in biological materials - Google Patents

Method and set for rift valley virus detection and antibodies to its in biological materials

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Publication number
RU94020434A
RU94020434A RU94020434/14A RU94020434A RU94020434A RU 94020434 A RU94020434 A RU 94020434A RU 94020434/14 A RU94020434/14 A RU 94020434/14A RU 94020434 A RU94020434 A RU 94020434A RU 94020434 A RU94020434 A RU 94020434A
Authority
RU
Russia
Prior art keywords
biotin
antibodies
virus
biological materials
igg
Prior art date
Application number
RU94020434/14A
Other languages
Russian (ru)
Other versions
RU2122210C1 (en
Inventor
Л.Ю. Вергун
И.Ф. Вишняков
Original Assignee
Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии filed Critical Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии
Priority to RU94020434A priority Critical patent/RU2122210C1/en
Publication of RU94020434A publication Critical patent/RU94020434A/en
Application granted granted Critical
Publication of RU2122210C1 publication Critical patent/RU2122210C1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/12011—Bunyaviridae
    • C12N2760/12211—Phlebovirus, e.g. Rift Valley fever virus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

FIELD: virology. SUBSTANCE: method ensures to detect antigen and antibodies to virus in biological materials by using the following complex: IgG-biotin + streptavidine + biotin -β-galactosidase at ratio of streptavidine and biotinylated enzyme = 2:1 or 1:1, respectively. Reaction sensitivity is increased by 25-500 times and provides the detection of 3 lg LD50/ml and 2 lg TCD50/ml virus. The proposed set consists of capacities containing the control and specific antigens; control and specific sera; conjugates: specific IgG-biotin, antispecies IgG-biotin, biotin-b-galactosidase; streptovidine. EFFECT: improved method of virus detection. 3 tbl

Claims (1)

Изобретение относится к вирусологии, в частности, к способу и набору для обнаружения вируса лихорадки долины Рифт (ЛДР) и антител к нему в биологических материалах, и может быть использовано в в биологической промышленности для наработки наборов. Цель: разработка способа и набора для обнаружения вируса ЛДР и антител к нему в биологических материалах (органных, культуральных, крови). Предлагаемый способ позволяет обнаруживать антиген и антитела к вирусу в биологических материалах путем применения комплекса IgG- биотин+стрептавидин+биотин-β-галактозидаза при соотношении стрептавидина и биотинилированного фермента 2:1 или 1:1 соответственно, благодаря этому чувствительность реакции повышается в 25-500 раз и обеспечивает обнаружение 3 lg ЛД 50/мл и 2 lg ТЦД 50/мл вируса. Предлагаемый набор включает емкости, содержащие контрольный и специфический антигены; контрольную и специфическую сыворотки; конъюгаты: специфический IgG-биотин, антивидовой IgG-биотин, биотин-b-галактозидаза, стрептавидин.The invention relates to virology, in particular, to a method and kit for detecting the Rift Valley fever virus (LDR) and antibodies to it in biological materials, and can be used in the biological industry to generate kits. Purpose: to develop a method and kit for detecting the LDR virus and antibodies to it in biological materials (organ, culture, blood). The proposed method allows to detect antigen and antibodies to the virus in biological materials by using the complex IgG-biotin + streptavidin + biotin-β-galactosidase with a ratio of streptavidin and biotinylated enzyme 2: 1 or 1: 1, respectively, due to this, the sensitivity of the reaction increases by 25-500 times and provides detection of 3 lg LD 50 / ml and 2 lg TCD 50 / ml virus. The proposed kit includes containers containing control and specific antigens; control and specific serum; conjugates: specific IgG-biotin, anti-species IgG-biotin, biotin-b-galactosidase, streptavidin.
RU94020434A 1994-05-26 1994-05-26 Method and set for detection of rift valley fever virus in biological material RU2122210C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94020434A RU2122210C1 (en) 1994-05-26 1994-05-26 Method and set for detection of rift valley fever virus in biological material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94020434A RU2122210C1 (en) 1994-05-26 1994-05-26 Method and set for detection of rift valley fever virus in biological material

Publications (2)

Publication Number Publication Date
RU94020434A true RU94020434A (en) 1996-11-27
RU2122210C1 RU2122210C1 (en) 1998-11-20

Family

ID=20156678

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94020434A RU2122210C1 (en) 1994-05-26 1994-05-26 Method and set for detection of rift valley fever virus in biological material

Country Status (1)

Country Link
RU (1) RU2122210C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111239414A (en) * 2020-01-21 2020-06-05 上海生物芯片有限公司 Kit for detecting IL-6 and method for detecting IL-6

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2457255C2 (en) * 2010-06-01 2012-07-27 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Россельхозакадемии Synthetic oligonucleotide primers, method for detecting and differentiating rift valley virus strains by polymerase chain reaction and restriction enzyme digest analysis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111239414A (en) * 2020-01-21 2020-06-05 上海生物芯片有限公司 Kit for detecting IL-6 and method for detecting IL-6
CN111239414B (en) * 2020-01-21 2023-09-12 上海生物芯片有限公司 Kit for detecting IL-6 and method for detecting IL-6

Also Published As

Publication number Publication date
RU2122210C1 (en) 1998-11-20

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