RU93048972A - PURVGF RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF THE L-IVP STRAW VACPUS VACCUS GROWTH FACTOR SYNTHESIS AND THE E. coli BACTERIA STRAIN - PRODUCER OF THE SUMMER - Google Patents

PURVGF RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF THE L-IVP STRAW VACPUS VACCUS GROWTH FACTOR SYNTHESIS AND THE E. coli BACTERIA STRAIN - PRODUCER OF THE SUMMER

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Publication number
RU93048972A
RU93048972A RU93048972/13A RU93048972A RU93048972A RU 93048972 A RU93048972 A RU 93048972A RU 93048972/13 A RU93048972/13 A RU 93048972/13A RU 93048972 A RU93048972 A RU 93048972A RU 93048972 A RU93048972 A RU 93048972A
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RU
Russia
Prior art keywords
strain
ivp
growth factor
synthesis
vgf
Prior art date
Application number
RU93048972/13A
Other languages
Russian (ru)
Other versions
RU2089612C1 (en
Inventor
В.С. Петров
П.А. Белавин
Н.В. Чешенко
В.В. Распопин
Н.А. Патрушев
А.В. Крендельщиков
Э.Г. Малыгин
Original Assignee
Научно-производственное объединение "Вектор"
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Publication date
Application filed by Научно-производственное объединение "Вектор" filed Critical Научно-производственное объединение "Вектор"
Priority to RU93048972A priority Critical patent/RU2089612C1/en
Priority claimed from RU93048972A external-priority patent/RU2089612C1/en
Publication of RU93048972A publication Critical patent/RU93048972A/en
Application granted granted Critical
Publication of RU2089612C1 publication Critical patent/RU2089612C1/en

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Claims (1)

Рекомбинантная плазмидная ДНК pUR VGF, кодирующая синтез фактора роста вируса осповакцины штамма Л-ИВП, и штамм бактерий Е.coli - продуцент фактор роста вируса осповакцины штамма Л-ИВП. Изобретение относится к области биотехнологии и медицины и представляет собой плазмидную конструкцию pUR VGF, обеспечивающую экспрессию фактора роста вируса осповакцины в виде "химерного" белка, слитого с бета-галактозидазой Е.coli, с молекулярной массой 127кД, взаимодействующего с моноспецифической антисывороткой и химически синтезированным пептидом, состоящим из 13 а.к.сN - конца зрелого VGF. Целевая конструкция содержит векторную часть в виде Hind III - BamHI фрагмента плазмиды pUR 290 размером 5.186 н.п. с генами ZacZ и Bla, а также BamHI - Hind III - фрагмент размером 233 н.п., содержащий последовательность открытой рамки считывания гена VGF вируса осповакцины штамма Л-ИВП клона 4 без последовательностей, кодирующих сигнальную и трансмембранную части гена. Уровень экспрессии "химерного" белка составляет 6 мг/грамм бактериальных клеток. Штамм-продуцент бактерий Е.coli ВМН7118 зарегистрирован во Всесоюзной коллекции промышленных микроорганизмов ВНИИ генетика под номером ВКПМ В-6566.Recombinant plasmid DNA pUR VGF, encoding the synthesis of growth factor virus vaccinia virus strain L-IVP, and the bacterial strain E. coli - producer of growth factor virus vaccinia strain L-IVP strain. The invention relates to the field of biotechnology and medicine and is a pUR VGF plasmid construct that expresses the vaccinia virus growth factor in the form of a “chimeric” protein fused to E. coli beta-galactosidase with a molecular weight of 127 kD interacting with a monospecific antiserum and chemically synthesized peptide consisting of 13 a.c.s.N - the end of mature VGF. The target construct contains the vector part in the form of a Hind III - BamHI fragment of plasmid pUR 290 with a size of 5.186 n.p. with the ZacZ and Bla genes, as well as the BamHI - Hind III - 233 bp fragment containing the open reading frame of the VGF gene of the vaccinia virus of the L-IVP strain of clone 4 without sequences encoding the signal and transmembrane parts of the gene. The expression level of the "chimeric" protein is 6 mg / gram of bacterial cells. The strain producing bacteria E. coli BMH7118 is registered in the All-Union Collection of Industrial Microorganisms of the All-Russian Research Institute of Genetics under the number VKPM B-6566.
RU93048972A 1993-10-25 1993-10-25 Recombinant plasmid dna purvgf determining synthesis of smallpox virus strain l-ivp growth factor together with escherichia coli beta-galactosidase and strain of bacterium escherichia coli containing recombinant plasmid dna purvgf - a producer of smallpox virus strain l-ivp growth factor RU2089612C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93048972A RU2089612C1 (en) 1993-10-25 1993-10-25 Recombinant plasmid dna purvgf determining synthesis of smallpox virus strain l-ivp growth factor together with escherichia coli beta-galactosidase and strain of bacterium escherichia coli containing recombinant plasmid dna purvgf - a producer of smallpox virus strain l-ivp growth factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93048972A RU2089612C1 (en) 1993-10-25 1993-10-25 Recombinant plasmid dna purvgf determining synthesis of smallpox virus strain l-ivp growth factor together with escherichia coli beta-galactosidase and strain of bacterium escherichia coli containing recombinant plasmid dna purvgf - a producer of smallpox virus strain l-ivp growth factor

Publications (2)

Publication Number Publication Date
RU93048972A true RU93048972A (en) 1997-02-10
RU2089612C1 RU2089612C1 (en) 1997-09-10

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RU93048972A RU2089612C1 (en) 1993-10-25 1993-10-25 Recombinant plasmid dna purvgf determining synthesis of smallpox virus strain l-ivp growth factor together with escherichia coli beta-galactosidase and strain of bacterium escherichia coli containing recombinant plasmid dna purvgf - a producer of smallpox virus strain l-ivp growth factor

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RU (1) RU2089612C1 (en)

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