RU92014708A - METHOD FOR ISOLATING CHONDROITINSULFATE - Google Patents

METHOD FOR ISOLATING CHONDROITINSULFATE

Info

Publication number
RU92014708A
RU92014708A RU92014708/14A RU92014708A RU92014708A RU 92014708 A RU92014708 A RU 92014708A RU 92014708/14 A RU92014708/14 A RU 92014708/14A RU 92014708 A RU92014708 A RU 92014708A RU 92014708 A RU92014708 A RU 92014708A
Authority
RU
Russia
Prior art keywords
aqueous solution
chondroitin sulfate
hydroxide
extraction
salt
Prior art date
Application number
RU92014708/14A
Other languages
Russian (ru)
Other versions
RU2061485C1 (en
Inventor
С.Е. Васюков
Н.А. Кирьянов
И.В. Лукина
А.А. Шульгина
Г.П. Животова
Original Assignee
С.Е. Васюков
Н.А. Кирьянов
Filing date
Publication date
Application filed by С.Е. Васюков, Н.А. Кирьянов filed Critical С.Е. Васюков
Priority to RU92014708A priority Critical patent/RU2061485C1/en
Priority claimed from RU92014708A external-priority patent/RU2061485C1/en
Application granted granted Critical
Publication of RU2061485C1 publication Critical patent/RU2061485C1/en
Publication of RU92014708A publication Critical patent/RU92014708A/en

Links

Claims (5)

1. Способ выделения хондроитинсульфата из животных тканей путем обезжиривания сырья нагреванием в воде при 60 - 95oC, экстракции хондроитинсульфата водным раствором хлорида натрия, сорбции на макропористом анионите, элюирования, осаждения и последующей очистки, отличающийся тем, что с целью повышения выхода целевого продукта, проводят обезжиривание животной ткани нагреванием в воде при 95 - 120oC, экстракцию хондроитинсульфата 0,05 - 1,1 М водным раствором гидроокиси при 10 - 70oC.1. The method of separation of chondroitin sulfate from animal tissues by degreasing the raw material by heating in water at 60 - 95 o C, extraction of chondroitin sulfate with an aqueous solution of sodium chloride, sorption on macroporous anion exchange resin, elution, precipitation and subsequent purification, characterized in that in order to increase the yield of the target product degrease animal tissue by heating in water at 95 - 120 o C, extraction of chondroitin sulfate 0.05 - 1.1 M aqueous solution of hydroxide at 10 - 70 o C. 2. Способ по п.1, отличающийся тем, что обезжиривание проводят нагреванием в 0,1 - 3,5 М водном растворе соли при 60 - 120oC.2. The method according to claim 1, characterized in that the degreasing is carried out by heating in a 0.1 - 3.5 M aqueous solution of salt at 60 - 120 o C. 3. Способ по пп. 1 и 2, отличающийся тем, что экстракцию хондроитинсульфата проводят водным раствором смеси гидроокиси (0,05 - 1,1 моль/л) и соли (0,1 - 3,5 моль/л). 3. The method according to PP. 1 and 2, characterized in that the extraction of chondroitin sulfate is carried out with an aqueous solution of a mixture of hydroxide (0.05 - 1.1 mol / l) and salt (0.1 - 3.5 mol / l). 4. Способ по пп. 1 - 3, отличающийся тем, что гидроокись выбрана из группы, включающей гидроокиси щелочных и щелочноземельных металлов. 4. The method according to PP. 1 to 3, characterized in that the hydroxide is selected from the group comprising hydroxides of alkali and alkaline earth metals. 5. Способ по пп. 2 и 3, отличающийся тем, что соль выбрана из группы, включающей хлориды, ацетаты, карбонаты или сульфаты щелочных и щелочноземельных металлов. 5. The method according to PP. 2 and 3, characterized in that the salt is selected from the group comprising chlorides, acetates, carbonates or sulfates of alkali and alkaline earth metals.
RU92014708A 1992-12-28 1992-12-28 Method of isolation of chondroitine sulfate from animal tissues RU2061485C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU92014708A RU2061485C1 (en) 1992-12-28 1992-12-28 Method of isolation of chondroitine sulfate from animal tissues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU92014708A RU2061485C1 (en) 1992-12-28 1992-12-28 Method of isolation of chondroitine sulfate from animal tissues

Publications (2)

Publication Number Publication Date
RU2061485C1 RU2061485C1 (en) 1996-06-10
RU92014708A true RU92014708A (en) 1998-06-10

Family

ID=20134402

Family Applications (1)

Application Number Title Priority Date Filing Date
RU92014708A RU2061485C1 (en) 1992-12-28 1992-12-28 Method of isolation of chondroitine sulfate from animal tissues

Country Status (1)

Country Link
RU (1) RU2061485C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2478652C1 (en) * 2011-11-25 2013-04-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Мурманский государственный технический университет" (ФГБОУВПО "МГТУ") Electrochemical method of purifying chondroitin sulphate
RU2693262C2 (en) * 2014-03-21 2019-07-01 Альтергон С.А. Method for purifying chondroitin sulphate

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