RU35440U1 - Optoelectronic diagnostic complex - Google Patents

Description

Optoelectronic diagnostic complex.

The utility model relates to medical equipment, namely to optical devices for diagnosis.

The prior art various devices for diagnostics using laser radiation (see, for example, patents RU-C1№2102925, 1996, RU-C1-No.2160897, 2000, applications FR-A-No.2436086 1981). The main disadvantage of the known optical devices for diagnosis is that they have limited capabilities.

Also known is an optical-electronic diagnostic complex, taken as a prototype and containing a block of optical radiation sources (lasers) optically coupled to the input of the first fiber-optic line, the output of which is the optical output of the complex, a spectrometer, the input of which is optically coupled to the output of the second fiber-optic line , the input of which is the optical input of the optoelectronic diagnostic complex. The spectrometer is made in the form of a polychromator based on narrow-band light filters, each of which is optically coupled by a corresponding photoconverter, the outputs of which are connected by an edotron switch to the ADC. In addition, the well-known complex includes a personal computer system unit, the input / output of which is connected to a video camera, and the input with an ADC output, which is the spectrometer output, the first output of the system unit is connected to the control input of the optical radiation source unit, and the second and third outputs are connected respectively, with the control input of the electronic commutator pp and the control input of the ADC, which are respectively) vsh1 and the second inputs of the spectrometer (see patent RU-C2-No. 2154398,

2 O O 3 1 2. 7, g 16

MPK7 GOIN 33/48

The disadvantages of the prototype:

-low detection ability due to the parallel accumulation of signals of the outputs of the polychromator;

low reliability of spectral data due to the low resolution of the spectrometer;

-low dynamic range;

- Lack of control over time and energy parameters of lasers.

The utility model is aimed at solving the technical problem of increasing the technical and operational parameters of the optoelectronic diagnostic complex.

The problem is solved in that the optical-electronic complex containing a block of optical radiation sources that are optically coupled to the input of the first fiber-optic line, a spectrometer, the input of which is optically coupled to the output of the second fiber-optic line and the processor unit, including the system unit of a personal computer, according to a utility model, it further comprises an optical measuring unit, the input and output of which are optically coupled, respectively, with the output of the first fiber optic line and the input of the WTO fiber optic line, the spectrometer is made in the form of a double acousto-optic monochromator, at the output of which there is a photoconverter connected with its output to the input of the signal preprocessing unit, the output of which is the output of the spectrometer, and the driver of the double acousto-optical monochromator, which is made in the form of two acousto-optical filters, and the output polarizer of the first acousto-optical filter is the input polarizer of the second acousto-optical about the filter, the first and second outputs of the driver of a double acousto-optic monochromator are connected respectively to the piezoelectric transducer of the acousto-optic cell of the first and

the piezoelectric transducer of the acousto-optic cell of the second of acousto-optic filters, and the driver output of the double acousto-optic monochromator is the input of the spectrometer, the processor unit further comprises a microprocessor, the input / output of which is connected to the serial port of the system unit of the personal computer, the interface of which is the first output of the processor unit, the input and, at least one microprocessor output, respectively, are the input and other outputs of the processor unit, the input which and its second output are connected respectively to the output and the first input of the spectrometer.

In addition, the task is solved in that:

the optical measuring unit contains an optical shutter, the control input of which is the first input of the optical measuring unit, while the third output of the processing unit is connected to the first input of the optical measuring unit;

the optical measuring unit contains means for smooth or stepwise adjustment of the laser acting on the sample located in the optical measuring unit, while the control input of the above means is the fourth input of the optical measuring unit and is connected to the fourth output of the processor unit;

- the signal preprocessing unit is configured to change the signal registration mode, while the control input of the signal preprocessing unit is the second input of the spectrometer and is connected to the fifth input of the processor unit;

- the signal preprocessing unit is configured to change the signal registration mode from the photon counting mode to the signal integration mode;

- the optical measuring unit includes an automatic test tube supply system, the control input of which is the third input of the optical measuring unit and is connected to the sixth output of the processor unit;

- the block of optical radiation sources contains at least two lasers connected to the corresponding power supplies, each of which is made with a control input;

- the block of optical radiation sources contains a laser operating at a wavelength of 440 nm, a laser operating at a wavelength of 530 nm and a laser operating at a wavelength of bZONm;

The control inputs of the laser power sources via a communication line are connected to the corresponding group of outputs of the processor unit.

The advantages of the proposed optical-electronic diagnostic complex over the known one is that it provides an increase in the detection ability due to the modulation of the investigated radiation and subsequent synchronous processing. In addition, an increase in dynamic range is provided. Additionally, it is possible to control the energy and time parameters of laser radiation. In the future, the utility model is illustrated by drawings and a description of them.

Figure 1 presents a block diagram of an optoelectronic diagnostic complex; figure 2, 3 and 4 - measurement schemes.

The optical-electronic diagnostic complex comprises an optical radiation source unit 1, an optical measuring unit 2 (OIB) and a spectrometer 3 connected optically in conjunction with each other and connected to the processor unit 4, which is connected to the display unit 5 for indicating and recording measurement results (figure 1).

Block 1 of the optical radiation sources includes at least two lasers. In a preferred embodiment, the implementation of the complex unit 1 of the sources of optical radiation contains connected to

the corresponding power supply units 6, 7 and 8, a laser 9 operating at a wavelength of 44S iHM, a laser 10 operating at a wavelength of 530 .W, and a laser 11 operating at a wavelength of 630 im. It is also possible to use in the proposed complex one power supply unit for all lasers with the number of outputs equal to the number of lasers used. The power supply unit 6 has a control input 6, and the blocks 7 and 8 have control inputs 7 and 8, respectively.

The output of each laser 9, 10 and 11 is optically coupled to the optical measuring unit 2 by means of the first fiber optic line 12.

The optical measuring unit 2 can be made in various ways, in particular, in the form of an integrating photometric camera equipped with a holder for a liquid cell. In a preferred embodiment, the optical-electronic diagnostic complex as an optical measuring unit 2 uses an automatic tube feeding system (ASPP) of the Eppendorf type.

The optical measuring unit 2 also includes an optical shutter 13 for interrupting the laser radiation acting on the sample, as well as means 14 for smoothly or stepwise adjusting the laser radiation acting on the sample.

Depending on the type of test sample 15, either a back reflection measurement circuit (FIG. 2) based on a Y-shaped fiber optic splitter 16 or a passage measurement circuit (FIG. 4) can be used.

The spectrometer 3, which is optically coupled to the OIB output by means of the second fiber-optic line 12, contains a double acousto-optic monochromator 17, at the output of which is installed, for example, a cooled photoconverter 18 (in the preferred embodiment, a PMT), connected by its output to the input of the signal preprocessing unit 19. In addition, spectrometer 3 contains

the driver 20 of the double acousto-optic monochromator 17, while the output of the signal preprocessing unit 19 is the output of the spectrometer 3, and the input of the driver 20 of the double acousto-optic monochromator 17 is its first (electrical) input. In the case of execution of the signal preprocessing unit 19 with the possibility of changing the signal registration mode from the output of the photoconverter 18 (from the photon counting mode to the signal integration mode in order to further expand the dynamic range by 20-30 dB), its control input is the second input of the spectrometer 3.

A double acousto-optic monochromator 17 contains three (first 21, second 22 and third 23) polarizers, as well as first 24 and second 25 acousto-optic cells (AOI), each of which includes a solid-state active medium 26 - a paratellurite crystal and a piezoelectric transducer 27 (ultrasonic emitter) .

The first polarizer 21 is installed at the input of the first AOI 24 so that the wave vector of the acoustic wave excited in the crystal 26 of the AOI 24 lies in the plane of polarization of the input light beam 28, and the second polarizer 22 crossed with respect to it is located perpendicular to the direction of propagation of the diffracted into the AOI 24 light beam 28. The second AOI 25 is located relative to the second polarizer 22 so that the wave vector of the acoustic wave excited in the AOI crystal 26 lies in the plane of polarization of its input light beam 29. The third polarizer 23, crossed relative to the second polarizer 22, is mounted orthogonal to the propagation direction of the light beam diffracted in AOI 25. The driver 20 of the double acousto-optic monochromator 17 includes a high-frequency synthesizer 30, the input of which is the input of the driver 20, and a high-frequency amplifier 31, to the first and the second outputs of which (which are respectively the first and second outputs of the driver 20) are connected respectively piezoelectric

converters 27 AOIA 24 and AOIA 25. The output of the high-frequency synthesizer 30 is connected to the input of the high-frequency amplifier 31.

The processor unit 4 includes a microprocessor 32 and a system unit 33 of a personal computer, to the interface of which a display unit 5 (display 34) and registration (printer 35) are connected to the measurement results. In other words, the interface of the system unit 33 is the first output of the processor unit 4. The input / output of the microprocessor 32 is connected to the serial port of the system unit 33 of the personal computer. The input, as well as the first, second and third outputs of the microprocessor 32 are respectively the input and the second, third and fourth outputs of the processor unit 4. The input of the processor unit 4 is connected to the output of the spectrometer 3. The second output of the processor unit 4 is connected to the first input of the spectrometer 3, and the third and the fourth outputs of the processor unit are connected respectively to the first and second inputs of the optical measuring unit 2, which are the control inputs of the optical shutter 13 and means 14 for smooth or step control, respectively Rovkov acting on a sample of 15 of the laser radiation. In the case of the need to provide two modes of recording the signal from the output of the photoconverter 18 (photon counting mode and signal integration mode), the microprocessor 32 is executed with an additional fourth output, which is the fifth output of the processor unit 4 and connected to the second input of the spectrometer 3. In the case of optical -measurement unit 2 in the form of an automatic tube supply system, for example, Eppendorf type, the processor unit 4 is performed with a sixth output, which is the corresponding fifth output of the microprocess ora 32 and connected to the third input of the optical-measuring unit 2, which is a control input of the automatic feed system of tubes. In some cases, it is advisable to execute the processor unit 4 (and therefore the microprocessor 32) with an additional group of outputs connected to the control inputs 6, 7.8 of the source unit 1

optical radiation (shown in FIG. dashed line) communication line 36.

The optical measuring unit 2 is optically coupled to the spectrometer using either a lens or a second fiber optic line 37.

Optoelectronic diagnostic complex operates as follows. In accordance with the control signals applied to one of the control inputs 6, 7 and 8 of block 1 of the optical radiation sources, as well as to the first and second inputs of the optical measuring block 2, the following is performed: the start of a certain laser 6-8 block 1 of the optical radiation sources, installation the required level of attenuation of the probe radiation (using means 14 for smooth or stepwise adjustment of the laser radiation acting on the sample 15) and control of the optical shutter 13. Here it should be noted that starting the desired (for Specific studies) of the laser 6-8 can be carried out either manually or automatically, namely, by signals transmitted via communication line 36, from the corresponding outputs of the processor unit 4. Thus, the laser radiation of the required spectral composition and intensity by means of a fiber optic line 12 at the required time is sent to the test sample 15 in accordance with a pre-selected measurement scheme (Fig. 2-4).

The laser radiation reflected or transmitted through the sample 15, as well as luminescence radiation (in the case of studying samples with luminescence centers), is supplied to the input of a double acousto-optic monochromator 17 made on the basis of two acousto-optic filters arranged in series with the second fiber optic line 37 the polarizer 22 of the first acousto-optical filter is the input polarizer of the second acousto-optical filter. Each acousto-optic filter (see Physical Encyclopedia, M. ed. Soviet Encyclopedia, vol. 1, p. 48, 1998) includes respectively AOIA 24 (AOIA 25), as well as an input

polarizer 21 (22) and output polarizer 22 (23). The first (input) polarizer 21 absorbs radiation having a linear polarization in accordance with the orientation of this polarizer. Using a piezoelectric transducer 27 (to which a mono-frequency high-frequency signal is supplied from the first output of the driver 20), a monochromatic sound of the same frequency is excited in the solid-state active medium 26 AOI 24, during the propagation of which (due to the elasto-optical effect) a volume diffraction grating is formed. Anisotropic Bragg diffraction in a narrow spectral range results in diffracted optical radiation of a different polarization (orthogonal to the input radiation), which is emitted by the output polarizer 22. The second acousto-optical filter also works. Thus, the signal from the second output of the processor unit 4 is fed to the input of the spectrometer 3 and starts the driver 20 of the double acousto-optic monochromator 17. As a result, the output of the high-frequency synthesizer 30 mono-frequency pulsed high-frequency signals are sequentially generated within the limits determined by the optical tuning range of the acousto-optic filters, and with a step depending on the width of the passband of acousto-optic filters. The amplified pulsed mono-frequency high-frequency signals are fed to the piezo-electric transducers 27 AOIA 24 and AOA 25. The pulse-modulated spectral component of the radiation transmitted to the input of the spectrometer 3, extracted using a double acousto-optical monochromator 17, is converted into a pulsed electrical signal using a photo-converter 18. The signal from the output of the photo-converter 18 enters the signal preprocessing unit 19, which provides two registration modes: photon counting mode and int mode signal aggregation. The selection of the registration mode is carried out according to the signals received at the second input of the spectrometer 3 from the fifth output of the processor unit 4. C

The output of the signal preprocessing unit 19, the signals are input to the processor unit 4, which provides the following parameters on the display 34: spectral density of the fluorescence energy brightness, relative fluorescence, fluorescence spectrum normalized to the reference sample, amplitude and half-width of the fluorescence spectrum.

The proposed optoelectronic diagnostic complex can be used for rapid study of the fluorescence spectra of aerobic, anaerobic bacteria, as well as for the optical diagnosis of diseases and microbial processes in medicine, industry, and food biotechnology.

Claims (10)

1. Optical-electronic complex containing a block of optical radiation sources that are optically coupled to the input of the first fiber-optic line, a spectrometer, the input of which is optically coupled to the output of the second fiber-optic line, and a processor unit including a personal computer system unit, characterized in that it additionally contains an optical measuring unit, the input and output of which are optically coupled, respectively, with the output of the first fiber optic line and the input of the second fiber optic line, a spectrum the meter is made in the form of a double acousto-optic monochromator, at the output of which there is a photoconverter connected with its output to the input of the signal preprocessing unit, the output of which is the output of the spectrometer, and the driver of the double acousto-optic monochromator, which is made in the form of two acousto-optic filters arranged in series, the output polarizer of the first acousto-optical filter is the input polarizer of the second acousto-optical filter, the first and second outputs drive the faith of the double acousto-optic monochromator is connected respectively to the piezoelectric transducer of the acousto-optic cell of the first and to the piezoelectric transducer of the acousto-optic cell of the second of the acousto-optic filters, and the driver output of the double acousto-optic monochromator is the input of the spectrometer, the processor unit additionally contains a microprocessor, the input / output of which is connected to the serial port of the personal system unit a computer whose interface is the first output m processing unit, input and at least one microprocessor output are respectively input, and other processing unit outputs the input-output processor unit having an input and a second output connected respectively to the output and the first input of the spectrometer. 2. The complex according to claim 1, characterized in that the optical measuring unit comprises an optical shutter, the control input of which is the first input of the optical measuring unit, while the third output of the processing unit is connected to the first input of the optical measuring unit. 3. The complex according to claim 1 or 2, characterized in that the optical measuring unit contains means for smooth or stepwise adjustment of laser radiation acting on the sample located in the optical measuring unit, while the control input of the above means is the fourth input of the optical measuring unit and is connected to the fourth output of the processor unit. 4. The complex according to claim 1, or 2, or 3, characterized in that the signal preprocessing unit is configured to change the signal registration mode, while the control input of the signal preprocessing unit is the second input of the spectrometer and connected to the fifth input of the processor unit. 5. The complex according to claim 4, characterized in that the signal preprocessing unit is configured to change the signal registration mode from the photon counting mode to the signal integration mode. 6. The complex according to claim 1, or 2, or 3, or 4, characterized in that the optical measuring unit includes an automatic tube feeding system, the control input of which is the third input of the optical measuring unit and connected to the sixth output of the processor unit. 7. The complex according to claim 1, characterized in that the block of optical radiation sources contains at least two lasers connected to respective power supplies, each of which is made with a control input. 8. The complex according to claim 7, characterized in that the block of optical radiation sources contains a laser operating at a wavelength of 0.44 μm, a laser operating at a wavelength of 0.53 μm, and a laser operating at a wavelength of 0.63 μm . 9. The complex according to claim 7 or 8, characterized in that the control inputs of the laser power sources via a communication line are connected to the corresponding group of outputs of the processor unit. 10. The complex according to claim 1, characterized in that it further comprises a unit for indicating and recording measurement results connected to the first output of the processor unit.