RU2811487C1 - Biological additive for introduction to healing products, healing compositions based on it - Google Patents

Abstract

FIELD: dermatology.

SUBSTANCE: healing agent for restoration, as well as care, moisturizing and nourishing of the skin for damage of various origins, dermatological diseases, metabolic disorders, stress and aging, accompanied by age-related changes in the skin, includes from 7 to 30 wt.% of a biological additive containing amino acids, vitamins, minerals salts, monosaccharides, mononucleotides in a certain amount.

EFFECT: invention provides a healing and restorative effect, relieves redness, inflammation and irritation, promotes the healing of minor injuries, cracks, abrasions, diaper rash, burns, wounds, trophic ulcers and even bedsores.

3 cl, 4 ex

Description

The invention relates to the field of cosmetology and dermatology.

The purpose of the invention is to create a complex biological additive (BD), the introduction of which into any acceptable base for external use, in particular into the composition of a cream, gel, lotion or ointment, ensures the replenishment of skin cells with physiologically active substances, similar in composition to those in healthy skin cells, and necessary for its functioning.

The essence of the invention

The composition of the multicomponent supplement, which is the subject of the invention, includes amino acids, vitamins, mineral salts, monosaccharides, mononucleotides, which provide the skin with natural substances and replenish their deficiency present in cells during stress, aging and skin damage of various origins.

The improved composition of the biological additive provides intensive yet natural nutrition, balanced hydration and healing of the skin, has high regenerating properties and is suitable for inclusion in a product for the care of any skin, in particular teenage, mature or damaged.

The composition is also suitable for use in products for sensitive skin, as it does not cause allergic reactions and provides comprehensive care.

A product containing the proposed biological additive, triggering skin restoration at the cellular level, has an excellent healing and restorative effect, ideal as a means for restoring and rejuvenating the skin, as well as for healing any type of damage. The use of a dietary supplement in ointments, creams, gels and liquid forms relieves redness, inflammation and irritation, promotes the healing of household burns, minor injuries, abrasions, diaper rash and even trophic wounds and bedsores.

Detailed Description of the Invention

The objective of the invention is to provide nutrition, hydration, normalization of the functioning of healthy, intact skin and/or healing of skin damage caused by various reasons. The technical result consists in the implementation of the task, as well as in expanding the arsenal of means for the specified purpose and is achieved through the multicomponent and multifunctional composition of the proposed biological additive.

The composition of the dietary supplement is based on the composition disclosed in the RF patent 2013088, many years of practical study of which led the author to the creation of a new, improved composition. Changes in the composition of the additive provided a significant improvement in the effect of the composition containing it on the condition of the skin, both healthy and damaged.

The differences from the composition of the biological additive disclosed in the RF patent 2013088 and taken as a prototype are that in order to increase the physiological activity of the declared biological additive in relation to the restoration of skin cells during stress and aging, as well as accelerating the skin healing process for cuts, scratches, abrasions, burns, frostbite, accelerating the healing of wounds cleared of infection, bedsores and trophic ulcers, the new composition additionally includes:

1. i-inositol;

2. menadione sodium bisulfite trihydrate;

3. 5-AMP;

4. iron sulfate heptahydrate FeSO ₄ *7H2O;

5. zinc sulfate heptahydrate ZnSO ₄ *7H2O;

6. copper sulfate pentahydrate CuSO ₄ *5H2O;

7. manganese chloride tetrahydrate MnCl*4H2O.

I - inositol helps to intensify the transmission of cellular signal impulses in response to hormones and growth factors.

Menadione sodium bisulfite trihydrate is used for growing human skin fragments outside the body (in vitro) after surgical operations. The hemostatic effect, in turn, appears to contribute to skin regeneration and wound healing.

5-AMP is a mononucleotide, adenosine monophosphate, plays an important role in many cellular metabolic processes.

Copper, zinc, iron and manganese sulfates are essential microelements for the restoration and intensive growth of mammalian cells in serum-free media.

One of the mechanisms of action of the above microelements on the restoration and renewal of cells can be considered their presence as a structural component in many enzyme and coenzyme systems that determine the quality of cellular respiration. Iron is an oxygen transmitter and is part of the respiratory enzyme, and copper is part of cytochrome oxidase, oxygenases and porphyrins, which, in turn, contributes to these processes. Zinc and manganese are components of enzymes that affect phosphorus, carbohydrate, and protein metabolism, as well as the regulation of redox potential in cells. In addition, zinc enhances the biosynthesis of nucleic acids and affects the activity of nucleic acid metabolism enzymes. Among the known enzymes, more than 40 contain zinc. The ability of manganese to ensure the stability of the structure of cell membranes also deserves attention.

In addition, the new composition of dietary supplements:

1. L cystine has been replaced with a more stable disodium salt of L cystine.

2. sodium bicarbonate is excluded, other ingredients are used to create a buffer environment that is more physiological for skin cells.

3. vitamins A and E are excluded; if desired, they can be quantum satis added to the composition of the target, final composition.

4. Nitrate decahydrate is excluded from the iron composition, which is replaced by a more bioactive iron sulfate.

The changes made to the new composition of the dietary supplement are aimed at intensifying nutrition, hydration, restoration of the skin under stress, aging and damage of various origins, as a result of providing skin cells with a more balanced, necessary and sufficient amount of natural nano-sized components (Chekman I.S., Syrovaya A.O. ., Novikova I.V., Makarov V.A., Andreeva S.V., Shapoval L.G. “Amino acids - nano-sized molecules: clinical laboratory studies”, Kharkov, 2014, p. 14), which are building materials for human skin cells. These nano-sized components can be classified as natural nanoparticles intended for the restoration and synthesis of mammalian cells, including skin cells.

An important factor is that the components of the dietary supplement, nanosized and natural, do not cause allergies and easily penetrate into the deep layers of the skin. In particular, this applies to both the products of the cosmetic line and the Regenerator line, which in its purpose is comparable to the healing drug “solcoseryl”, taken as the prototype of the Regenerator group products.

Solcoseryl contains as an active substance an extract from the blood of dairy calves - deproteinized hemodialysate, which is obtained by passing through a filtration system (dialysis with ultrafiltration), resulting in the formation of a pyrogen-free and hypoallergenic fraction, devoid of large proteins, containing a wide range of low-molecular components of cell mass and serum with a molecular weight of 5000 Da, and containing, among other things, glycoproteins (complex proteins containing, in addition to a simple protein or peptide, a group of heterooligosaccharides); nucleosides (glycosylamines containing a nitrogenous base bound to monosaccharides (ribose or deoxyribose); nucleotides (a group of organic compounds that are phosphorus esters of nucleosides); oligopeptides consisting of 10-50 amino acids and amino acids used in the synthesis of proteins and many other large biomolecules .

Nano-sized biomolecules included in the new dietary supplement have a molecular weight lower than that in deproteinized solcoseryl hemodialysate:

*amino acids, the linear size of which is less than 1 nm, and the molecular weight is in the range of 75-185 g/mol;

* monosaccharides, the molecular weight of which is on average 150-180 g/mol;

* mononucleotides with a molecular weight within 345 g/mol;

* vitamins with a molecular weight in the range of g/mol 155-524 g/mol;

* iron sulfate heptahydrate 151 g/mol, etc.

As can be seen from the data presented, the nano-sized molecules included in the new bioadditive have a molecular weight significantly lower than the molecular weight of the biomolecules included in the “solcoseryl” preparations (5000 Da). Nano-sized particles of solcoseryl include amino acids and, possibly, a number of other molecules.

In addition, obtaining deproteinized hemodialysate from the blood of dairy calves does not guarantee its identity, and may differ from batch to batch, since the set of molecular components in the blood of calves of different litters may be different.

A comparative analysis of the active component solcoseryl and the proposed new dietary supplement allows us to conclude that the new dietary supplement:

1. Non-pyrogenic and hypoallergenic, as it is formed exclusively from nano-sized biomolecules natural to human skin, which do not cause allergies or fever.

2. Initially does not contain components of cell mass and serum, as well as other intermediate metabolic compounds with a molecular weight of about 5000 Da (which significantly exceeds that of nano-sized biomolecules included in the new dietary supplement).

3. It has a wide spectrum, but at the same time contains a strictly defined composition of nano-sized biomolecules, which is extremely important for achieving the predicted effect of drugs, both cosmetic and regenerator group drugs created on its basis.

Composition of the proposed biological additive according to the invention in mg/l:

1.L-alanine 24-25;

2. L-arginine chloride 68-70;

3. aspartic acid 29-30;

4.L-cysteine chloride 0.0989-0.1;

5.L-cystine disodium salt 0.005-0.1;

6.L-glutamic acid 69-70;

7.L-glutamine 99-100;

8. glutathione 0.04-0.06;

9. glycine 48-50;

10.L-histidine chloride monohydrate 21.5-22;

11.L-hydroxyproline 9.5-10;

12.L-isoleucine 16-25;

13.L-leucine 58-60;

14.L-lysine chloride 65-70;

15.L-methionine 12-15;

16.L-phenylalanine 23-25;

17.L-proline 36-41;

18.L-serine 24-28;

19.L-threonine 25-30;

20.L-tryptophan 9-12;

21.L-tyrosine disodium salt 49-58;

22.L-valine 23-28;

23.L-ascorbic acid 0.04-0.07;

24.biotin 0.005-0.01;

25. calciferol 0.05-0.1;

26.D-calcium pantothenate 0.008-0.01;

27.choline chloride 0.4-1.0;

28. folic acid 0.005-0.01;

29.i-inositol 0.04-0.05;

30.menadione sodium bisulfite trihydrate 0.016-0.019;

31.nicotinic acid 0.02-0.025;

32.nicotinamide 0.02-0.025;

33. para-amino-benzoic acid 0.03-0.05;

34.pyridoxal chloride 0.02-0.025;

35.pyridoxine chloride 0.02-0.025;

36.riboflavin 0.008-0.01;

37.thiamine chloride 0.009-0.01;

38. calcium chloride 140-185.5;

39.potassium chloride 395-400;

40.potassium dihydrogen orthophosphate 58-60;

41.magnesium sulfate heptahydrate 121.9-122.4;

42. sodium chloride 7950-8000;

43.sodium hydrogen orthophosphate 47.5-47.69;

44.D-glucose 988-1000;

45.magnesium chloride hexahydrate 59-60

46.iron sulfate heptahydrate 0.41-1.39

47.copper sulfate pentahydrate 0.0012-0.0013

48.zinc sulfate heptahydrate 0.42-0.43

49.manganese chloride tetrahydrate 0.00005-0.0001

50.adenine sulfate 9.8-10;

51. 5-AMP 0.15-0.2;

52. ATP disodium salt 0.95-0.1;

53.cholesterol 0.18-0.2;

54.2-deoxyribose 0.45-0.5;

55. guanine chloride 0.27-0.3;

56.hypoxanthine 0.29-0.31;

57.D-ribose 0.48-0.5;

58.sodium acetate 35.9-36.7;

59.thymine 0.28-0.3;

60.twin 5.0-10.0;

61.uracil 0.25-03;

62. xanthine 0.27-03;

63.Water - the rest up to 1 liter.

The declared bioadditive is introduced into the composition of cosmetic and regenerating products (creams, ointments, gels, liquid forms) in an amount from 0.01 to 30 wt%. It should be clear to a specialist that the specific amount of the administered bioadditive depends, in particular, both on the purpose of the target cosmetic or dermatological product and on the selected form, that is, on the possibility of introducing the appropriate amount of the aqueous phase of the BD into the selected base.

The above formulation of the dietary supplement is prepared under sterile conditions, based on a balanced salt solution with sufficient buffer capacity that meets the physiological needs of mammalian cells, for example, Hanks solution, which is a mixture of inorganic salts dissolved in purified water (NaCl, KCI, MgSO ₄ , MgCl, KH ₂ PO ₄ , CaCI ₂ , Na ₂ HPO ₄ ), and glucose, sterilized by filtration through a system of membrane filters, with pH: from 6.8 to 7.2 and is used as a solvent for dry preparations and in the practice of cell culture, dilution of serums and avg.

experimental part

The process of obtaining BD is carried out under strictly sterile conditions in a specially equipped laboratory or laminar flow hood. To obtain a physiologically active dietary supplement in 800 ml. Hanks buffer saline solution, previously diluted 10 times with double-distilled sterile water, is successively dissolved in mg portions. the organic and inorganic substances listed below, with the exception of components NN 38-45 and 63 already contained in Hanks’ solution. After introducing all components, the dietary supplement is diluted with Hanks’ solution (diluted 10 times) to 1 liter, respectively, the amount of components in the dietary supplement is expressed in mg/ liter. The amount of dietary supplement is prepared depending on the planned preparation of a cosmetic or regenerating product. The remaining unused dietary supplement containing all of the above substances, if necessary, is poured into sterile 0.5 liter bottles, hermetically sealed and stored in the refrigerator until use.

Example of the composition of a dietary supplement in mg/liter:

1. L-alanine 25;

2. L-arginine chloride 70;

3. aspartic acid 30;

4. L-cysteine chloride 0.09;

5. L-cystine disodium salt 0.1;

6. L-glutamic acid 70;

7. L-glutamine 95;

8. glutathione 0.05;

9. glycine 50;

10. L-histidine chloride monohydrate 21.7;

11. L-hydroxyproline 9.5;

12. L-isoleucine 20;

13. L-leucine 60;

14. L-lysine chloride 65;

15. L-methionine 15;

16. L-phenylalanine 25;

17. L-proline 40;

18. L-serine 25;

19. L-threonine 30;

20. L-tryptophan 10;

21. L-tyrosine disodium salt 55;

22. L-valine 25;

23. L-ascorbic acid 0.05;

24. biotin 0.01;

25. calciferol 0.1;

26. D-calcium pantothenate 0.01;

27. choline chloride 0.5;

28. folic acid 0.01;

29. i-inositol 0.05;

30. Menadione sodium bisulfite trihydrate 0.017;

31. nicotinic acid 0.02;

32. nicotinamide 0.025;

33. para-amino-benzoic acid 0.04;

34. pyridoxal chloride 0.025;

35. pyridoxine chloride 0.025;

36. riboflavin 0.01;

37. thiamine chloride 0.01;

38. calcium chloride dihydrate 185;

39. potassium chloride 400;

40. potassium dihydrogen orthophosphate 60;

41. Magnesium sulfate heptahydrate 122;

42. sodium chloride 8000;

43. sodium hydroorthophosphate 47.5;

44. D-glucose 1000;

45. Magnesium chloride hexahydrate 60

46. iron sulfate heptahydrate 1.0

47. copper sulfate pentahydrate 0.0013

48. Zinc sulfate heptahydrate 0.43

49. manganese chloride tetrahydrate 0.0001

50. adenine sulfate 10;

51. 5-AMP 0.15;

52. ATP disodium salt 0.1;

53. cholesterol 0.2;

54. 2-deoxyribose 0.45;

55. guanine chloride 0.3;

56. hypoxanthine 0.3;

57. D-ribose 0.5;

58. sodium acetate 36.0;

59. thymine 0.3;

60. twin 7.5;

61. uracil 0.25;

62. xanthine 0.3;

63. Water - the rest up to 1 liter.

The composition includes a set of chemically pure amino acids, vitamins, minerals, monosaccharides and other organic and inorganic ingredients necessary for the life and restoration of cellular structures of the skin.

Examples of ready-made forms including bioadditives and methods for their preparation are given below.

Examples of ready-made forms:

1.Gel

Recipe

p/p Name
ingredients Introduction % wt/g 1 Carbopol 0.5%/30g 2 Triethanolamine 0.5%/30g 3 Glycerol 6%/360g 4 Akiporox 0.3%/18g Dietary supplement 0.5%/30g 6 Preservative Microcare DMP 0.9%/54g 7 Fragrance “Ginger” 0.3%/18g 8 Alcohol 96% 2.4%/144g 9 Dye green 0.1%/6g 10 Distilled water Up to 100%/up to 6kg

The preparation technology is designed to produce 6 kg of finished product in laboratory conditions.

1. The day before prepare:

In a 10 liter container, heat 1.5 liters of distilled water to 45 ⁰ C, pour 30 g of carbopol onto the surface of the heated water, and, without stirring, leave it to swell for a day.

300 ml of water is heated to 45-55 ⁰ C and 18 g of akiporox are dissolved in it and left overnight.

2. The next day, prepare the following solutions (the amount of water is taken from the total amount of the recipe):

c) The dietary supplement is dissolved in 28 ml of distilled water and combined with 360 g of glycerin.

d) A sample of fragrance is poured into a sample of 96% alcohol.

e) 30g. TEA is dissolved in 30 ml. water.

3. The carbopol soaked the day before is heated to 45 ⁰ C, mixed thoroughly and filtered through a clean metal sieve.

The resulting carbopol gel is pressed through a sieve with a clean wooden spatula or plastic spoon.

4. Add dye and mix thoroughly.

5. Add a mixture of glycerin + BD + water to the akiporox soaked the day before. Mix and pour into a solution of carbopol with dye. Mix well with a mixer at 1st speed.

6. Add the prepared portion of the preservative and mix thoroughly with a mixer for 3 minutes.

7. Slowly pour in the TEA/water mixture and continue mixing at low speed. The gelling process is underway.

8. Add a solution of fragrance and alcohol and mix thoroughly for 5-10 minutes. Leave it for a day.

9. The finished gel is packaged.

2.CREAM

The recipe and cooking technology are designed to produce 30 kg of finished product.

Phase p/p Name of ingredients Introduction
In mass/% Weight per 30 kg Fat 1 Bone marrow fat 2.25% 675g 2 Emulsion wax 4.5% 1350g 3 PEG400 stearate 2.0% 600g 4 Dimethicone 1.3% 390g 5 Cetiaryl alcohol 0.25% 75g 6 Soybean oil 1% 300g 7 Caprylic/capric triglycerides 1% 300g 8 Cacao butter 1.5% 450g 9 Vaseline oil 2% 600g water 1 Distilled water 74.425% 22327.50g 2 Glycerol 4.0% 1200g supplements 1 Preservative Microcare DMP 0.9% 270g 2 Alcohol 96% 2.0% 600 3 Dietary supplement 1.5% 45g 4 Vitamin E 0.5% 150g 5 Fragrance Intimate 0.3% 90g 7 Vitamin A 0.5% 150g 9 Citric acid (3% solution) 0.075% 22.5g

Cooking technology .

Set the heating temperature of the product in the boiler to 73 ⁰ C (in the “jacket” - 77 ⁰ C), the cooling temperature to 25 ⁰ C. Pour 22327.50 g of water passed through the filter system into the boiler, add glycerin and turn on the heating while stirring with a stirrer (50 rpm). In the boiler for melting fat, the fat phase is heated to 80 ⁰ C, after which the water phase is poured. Turn on the homogenizer for 8-10 minutes. Then turn on cooling while continuing stirring. At a temperature of 40 ⁰ C, alcohol and a preservative are added, at 35 ⁰ C - a dietary supplement, vitamins, and a 3% solution of citric acid. Fragrance is added at temperatures below 30 degrees. Cool the cream to 25-26°C and pour into a container for subsequent packaging.

3. LOTION to strengthen and stimulate hair growth for dry and normal skin

p/p Name
ingredients % mass For 10 kg For 20 kg 1 Dietary supplement 7% 700 g 1400g 2 Ethyl alcohol 96% 4% 400 g 800 g 3 Akiporox 0.5% 50 g 100 g 4 Preservative Microcare DMP 0.9% 90 g 180 g 5 Menthol 0.02% 2 g 4 g 6 Water 83.61% 8361g 16722 g 7 Glycerol 3.97% 397g 794g

Cooking technology

1. Akiporox is diluted in water taken from the total weight (volume) of water heated to a temperature of 40 ⁰ C. Water is taken at the rate of 350 g of water per 25 g of akiporox. Accordingly, with an increase in the amount of akiporox, the amount of water for its dilution increases proportionally.

2. Menthol is dissolved in alcohol

3. Dissolved akiporox is poured into the total amount of water. Stir.

4. Pour in alcohol with menthol. Stir.

5. Pour in glycerin. Stir.

6. Add a dietary supplement. Stir.

7. Add a preservative. Stir.

8. Leave for an hour and pour into bottles.

Regenerating products (regenerator cream, ointment, gel and lotion).

These drugs belong to the clinical-pharmacological group “Drugs that activate metabolism in tissues, improve trophism and stimulate the regeneration process.”

Pharmacological action: stimulator of tissue regeneration.

Forms of release of regenerating agents - tissue regeneration stimulators:

1. Ointment regenerator

2. Regenerator cream

3. Gel regenerator

4.Lotion regenerator

A new bioadditive is introduced into the above products, which is a wide range of chemically pure nanoscale components - amino acids, monosaccharides, mononucleotides, mineral salts, vitamins (see above the composition of the new biological additive) in an amount of 7 to 30 wt%. The Regenerator line of products activates regenerative processes in tissues, stimulates cell proliferation and collagen synthesis. Activation of cellular repair is accompanied by improved transport of oxygen and glucose to cells under hypoxic conditions, increases the synthesis of intracellular ATP and promotes an increase in aerobic glycolysis and oxidative phosphorylation.

The drugs do not cause allergies, since the dietary supplement does not contain proteins or other high-molecular components.

Examples of preparing preparations from the regenerator series.

4. OINTMENT regenerator

The recipe and cooking technology are designed to produce 25 kg of finished product.

Phase p/p Name
ingredients Number of mass% Weight Unit
measurement
leniya Way
measurements AND
AND
R
ABOUT
IN
A
I 1 Bone marrow fat 55% 13750 G Scales 2 Sorbitan oleate 1.5% 375 G Scales 3 Beeswax 2% 500 G Scales 4 Pentol 2.7% 675 G Scales 5 Cacao butter 3% 750 G Scales IN
ABOUT
D
N
A
I 1 Distilled water 21.4% 5350 G Graduated capacity 2 Glycerol 2.7% 675 G Scales D
ABOUT
B
A
IN
TO
And 1 Dietary supplement 8% 2000 G Sterile graduated
utensils (cylinder). 2 Vitamin E 0.5% 125 G Scales 3 Alcohol 2.7% 675 G Scales 4 Preservative Microcare DMP 0.5% 125 G Scales

COOKING TECHNOLOGY

In separate containers, the water and fat phases are heated to 80 ⁰ C. The heating temperature of the product in the boiler is set to 73 ⁰ C, the temperature in the “jacket” of the boiler is 77 ⁰ C. After reaching the desired temperature, the water phase is poured into the boiler, then the fat phase. Close the boiler lid and turn on the stirrer. At a temperature of 40 ⁰ C, a preservative and alcohol are added. At 35 ⁰ C, a dietary supplement and vitamin E are introduced. Cooling is continued to 27-28 ⁰ C, after which the finished product is poured into containers for packaging.

5. Cream - regenerator.

The cream is obtained in a similar way to ointment, calculating the load depending on the target amount of the finished product.

Name % mass mass gr. Deionized water Up to 100 Up to 100 soybean oil 4.1 4.1 caprylic/caproic acid triglyceride (rofetan) 2.3 2.3 ceteareth S2
(Massocare S2) 2.5 2.5 Lecithin
Lipoid s20(Lipoid GmbH) 1.1 1.1 glycerol 1.5 1.5 dimethicone 1.2 1.2 DIETARY SUPPLEMENT 7.0 7.0 Squalane
(Massocare SQV) 2.3 2.3 Preservative (Germaben II) 0.6 0.6 Conjak manane 0.8 0.8 Vitamin E 0.5 0.5 Fragrance
AR Ginseng 62805 0.11 0.11

Stage A. Preparation of the oil phase.

Pre-weighed amounts of ceteareth S2, soybean oil, rofetan and squalane are added to the fat melter, heated to a temperature of 70-80°C until the components are completely melted, after which 0.4% lecithin is added while stirring, avoiding its burning.

Step B. Preparation of the aqueous phase.

A pre-measured amount of water and glycerin is heated to a temperature of 70-80 °C, and 0.7% of the calculated lecithin is added.

Stage B. Preparation of the emulsion base.

Composition obtained fromstage Aadd to the composition obtained atstage B and homogenize for 45 minutes.

After cooling to 40°C, dimethicone is added to this system and stirring is continued for 10 minutes.

Stage D. Introduction of the biophase.

Pre-weighed amounts of preservative, bioadditive, Conjak manane, fragrance, vitamin E are added to the finished emulsion base and mixed at a speed of up to 2000 rpm for 10 minutes.

To prepare the regenerator gel and lotion, use (as an example) the above-mentioned Gel or Lotion recipe, respectively, but the amount of bioadditive used is approximately 9-15% wt. in lotion and 7-8% wt. in gel. It should be understood that the examples given are illustrative only and the auxiliary ingredients and their amounts may vary during formulation, in accordance with accepted practices and principles in the art. At the same time, the composition of the new dietary supplement remains unchanged.

Indications for use of the Regenerator product line, in particular ointments and gels, are:

minor skin damage: cuts, abrasions, scratches

1st and 2nd degree burns (sun and thermal burns);

frostbite

difficult-to-heal wounds (including trophic ulcers and bedsores).

For the treatment of trophic tissue lesions of various origins, Regenerator should be used only after removal of necrotic tissue and complete cleansing of the wound from infectious flora.

Regenerator Gel is used in the initial stage of treatment and is applied to fresh wounds, wounds with wet discharge or oozing ulcers.

Regenerator ointment is used primarily for the treatment of dry, non-wetting wounds and household burns that have been cleared of infection (before opening the blisters) - immediately after the burn, rinse the skin with a stream of cold water and apply the ointment in a thick layer.

In addition, regenerator products can be used as an additional remedy in the treatment of dermatological diseases (neurodermatitis, eczema, rosacea, psoriasis, etc.)

Examples of application of the compositions according to the invention

A. Application of the cream and gel according to the invention:

35 volunteers aged from 14 to 65 years took part in the study over the course of a month (9 subjects with dry skin used the cream; 7 with combination skin combined cream with gel; 12 with a tendency to oily skin used the gel and 7 with skin prone to irritation, alternated both products). According to the completed questionnaires, after 28 days of daily use of the cream and gel, according to the invention, 33 subjects noted a pronounced positive effect: the skin became denser, more elastic and toned, the skin tone evened out, redness and irritation decreased, and fine wrinkles disappeared.

B. Research on hair strengthening lotion:

20 volunteers aged from 25 to 40 years took part in the study over a 2-month period: 7 subjects with severe symptoms of hair loss and 13 with complaints of thinning and weakening. According to objective observations, including a description of the hairline, as well as completed questionnaires, after 30 days of applying the lotion in the form of a mask at night on washed hair, rubbing it into the scalp with massage movements once every three days (after application, put on a scarf and a shower cap, in the morning, without rinsing, comb your hair). 14 subjects noted a pronounced positive effect: their hair became denser, shiny and elastic. After 2 months, 19 subjects showed a decrease in hair loss.

C. Comparative studies

The effect when using the above-mentioned ready-made forms was compared with similar forms containing the composition previously disclosed in RF2013088.

The comparative tests involved 28 volunteers, including 3 groups of subjects according to skin type, without significant redness and damage (8 subjects with dry skin; 12 with combination skin; 8 with a tendency to oily skin), using the cream and gel in the “ as needed” mode. within a month. The group of subjects was divided into 2 equal research groups of 14 people each (4+6+4, respectively), one of which used the products according to this invention, the other a cream according to the prototype RF2013088 and a gel of the above formulation, but obtained using the additive from the above patent.

The subjects were provided with questionnaires (including, in particular, an assessment of the overall comfort of sensations, skin moisture, excessive oiliness, dryness, tightness, appearance and color of the skin, the condition of wrinkles) in which, on a ten-point scale, they had to weekly evaluate the effect of the products used.

As a result of comparative tests of the cream and gel obtained by introducing the claimed and previously disclosed biological additive into the corresponding base, an unexpected effect was expressed in a noticeable increase in skin density and elasticity, improvement of complexion and smoothing out of fine wrinkles noted by the group that used the products according to the present invention, in comparison with the group that used the cream obtained according to examples 1 and 2 of the above prototype (RU2013088) and a gel containing the additive according to the prototype.

D. Regenerator Product Research

D1. Lotion regenerator

The healing effect was studied on 9 volunteers aged from 20 to 50 years with skin prone to irritation and diaper rash. After 10 days of use, eight out of nine noted an improvement in their skin condition. The first positive results for the majority appeared on the 3rd day of using the regenerator lotion.

D2. Regenerator ointment

The study involved 5 patients with trophic ulcers cleared of infection, 2 with bedsores and 2 with 1st degree thermal burns.

In all patients with dry trophic ulcers and 1st degree household burns, by accelerating skin regeneration processes, it was possible to achieve improvement and pronounced signs of healing already on the 5th day of 2 times application of the ointment.

The same applies to one 67-year-old patient with bedsores. In the second patient, 76 years old, the effect was expressed in the stabilization of the condition of the skin, the bedsore stopped increasing in size, and no new lesions appeared on the areas of the skin adjacent to the bedsore, also treated with regenerator ointment.

D3. Cream and gel regenerator

The drug was tested as volunteers by employees and relatives of the author of the invention. Gel regenerator has proven itself to be a product that accelerates the healing process of the skin for scratches, abrasions, and minor burns (1st degree). The same applies to the regenerator cream.

Both drugs can be used independently or as an addition to regenerator ointment.

E. Comparative studies of the regenerator product line

E1. Patients with trophic ulcers and caregivers of patients with bedsores noted that the effectiveness of regenerator ointment was at the level of that of solcoseryl, which they had previously used, and even exceeded it.

E2. In addition, the effects of regenerator cream and gel on the skin of subjects with problem skin, constant irritation, and a tendency to allergic reactions and redness were studied. 24 volunteers aged 14 to 65 years received their choice of cream, gel or both products, with the possibility of combining them, and corresponding assessment questionnaires. The comparison groups, as far as possible, were formed as similar in age and skin condition.

The results of the impact on damaged skin of two comparison groups showed that the cream according to the invention led to the healing of irritations and redness 2 times more effectively, i.e. regenerating, restorative and soothing, the effect manifested itself almost 2 times faster (the effect took place 2-4 days from the start of the study), in comparison with the compositions according to the prototype, for which the first mention of such an effect refers to the period (5-7 days from start of the study).

conclusions

The above data proves the unexpected technical result of the new composition of the dietary supplement, namely the effect of improving the impact on healthy, teenage, age-related, problematic skin prone to redness and irritation, relieving symptoms of tightness, itching and discomfort, as well as accelerated healing of its damage of various origins. Thus, the new dietary supplement provides the opportunity to create new products that have a restorative and healing effect.

Claims (4)

1. A healing agent for restoration, as well as care, moisturizing and nourishing of the skin for damage of various origins, dermatological diseases, metabolic disorders, stress and aging, accompanied by age-related changes in the skin, including from 7 to 30 wt.% of a biological additive including in mg/l : L-alanine 24-25 L-arginine chloride 68-70 aspartic acid 29-30 L-cysteine chloride 0.0989-0.1 L-cystine disodium salt 0.005-0.1 L-glutamic acid 69-70 L-glutamine 99-100 glutathione 0.04-0.06 glycine 48-50 L-histidine chloride monohydrate 21.5-22 L-hydroxyproline 9.5-10 L-isoleucine 16-25 L-leucine 58-60 L-lysine chloride 65-70 L-methionine 12-15 L-phenylalanine 23-25 L-proline 36-41 L-serine 24-28 L-threonine 25-30 L-tryptophan 9-12 L-tyrosine disodium salt 49-58 L-valine 23-28 L-ascorbic acid 0.04-0.07 biotin 0.005-0.01 calciferol 0.05-0.1 D-calcium pantothenate 0.008-0.01 choline chloride 0.4-1.0 folic acid 0.005-0.01 i-inositol 0.04-0.05 Menadione sodium bisulfite trihydrate 0.016-0.019 a nicotinic acid 0.02-0.025 nicotinamide 0.02-0.025 para-amino-benzoic acid 0.03-0.05 pyridoxal chloride 0.02-0.025 pyridoxine chloride 0.02-0.025 riboflavin 0.008-0.01 thiamine chloride 0.009-0.01 calcium chloride 140-185.5 potassium chloride 395-400 potassium dihydrogen orthophosphate 58-60 magnesium sulfate heptahydrate 121.9-122.4 sodium chloride 7950-8000 sodium hydrogen phosphate 47.5-47.69 D-glucose 988-1000 magnesium chloride hexahydrate 59-60 iron sulfate heptadic 0.41-1.39 copper sulfate pentahydrate 0.0012-0.0013 zinc sulfate heptahydrate 0.42-0.43 manganese chloride tetrahydrate 0.00005-0.0001 adenine sulfate 9.8-10 5-AMP 0.15-0.2; ATP disodium salt 0.95-0.1; cholesterol 0.18-0.2 2-deoxyribose 0.45-0.5 guanine chloride 0.27-0.3 hypoxanthine 0.29-0.31 D-ribose 0.48-0.5 sodium acetate 35.9-36.7 thymine 0.28-0.3 twin 5.0-10.0 uracil 0.25-03 xanthine 0.27-03 Water rest 2. Healing agent according to claim 1, to accelerate the healing process of the skin with cuts, scratches, abrasions, burns, frostbite, accelerate the healing of wounds cleared of infection, bedsores and trophic ulcers. 3. Healing agent according to claim 2, in the form of lotion, gel, cream or ointment.