RU2800992C1 - Glutathione-producing yeast strain and a method of producing glutathione using the same - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention relates to a glutathione-producing strain of Saccharomyces cerevisiae under registration number KCCM12568P. The invention also relates to the production of glutathione using such a strain and the use of the strain in compositions for detoxification, to enhance immunity, in cosmetics, in food and feed.

EFFECT: obtaining glutathione with a high degree of efficiency.

15 cl, 3 dwg, 2 tbl, 6 ex

Description

1. Scope of invention

The present invention relates to a novel glutathione producing yeast strain and a method for producing glutathione using it.

2. Description of the prior art

Glutathione (GSH) is an organic sulfur compound most commonly found in cells and is in the form of a tripeptide in which three amino acids, glycine, glutamate and cysteine, are linked together.

Glutathione exists in the body in two forms: reduced glutathione (GSH) and oxidized glutathione (GSSG). Reduced glutathione (GSH), which under normal conditions is present in a relatively high percentage, is predominantly distributed in the cells of the liver and skin of the human body and performs important tasks, for example, it has an antioxidant function, decomposing and removing oxygen radicals, a detoxifying function, removing compounds of exogenous origin, such as toxic substances and so on, whitening function, inhibiting the production of melanin pigment, and so on.

Since the production of glutathione gradually decreases with age, the decrease in the production of glutathione, which has important antioxidant and detoxifying functions, contributes to the accumulation of oxygen radicals, which is one of the main causes of aging, and therefore the body needs to be supplied with glutathione from the outside (Sipes IG et al., The role of glutathione in the toxicity of xenobiotic compounds: metabolic activation of 1,2-dibromoethane by glutathione, Adv Exp Med Biol 1986;197: 457-67).

With such diverse functions, glutathione has received much attention as a material in various fields, such as pharmaceuticals, functional health foods, cosmetics, and so on, and is also used in the preparation of flavoring ingredients, foods, and feed additives. Glutathione is known to have a significant effect on enhancing the flavor of raw materials and maintaining a rich taste, and glutathione can be used as a "kokumi" flavor enhancer when used alone or in combination with other substances. In general, kokumi substances have a richer flavor than existing umami substances such as nucleic acids, MSG (monosodium glutamate) and so on, and are known to be derived from the degradation and aging of proteins.

However, despite the growing demand for glutathione, which can be used in various fields, as described above, the market is not significantly activated, since significant costs are required for the industrial production of glutathione.

Many efforts have been made to solve the above problems, and as a result, the inventors of the present invention have developed a new strain having an exceptional ability to produce glutathione, thereby completing the present invention.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a new strain of Saccharomyces cerevisiae that produces glutathione.

Another object of the present invention is to provide a method for producing glutathione, comprising the step of cultivating said strain.

Another object of the present invention is to provide a method for preparing a composition containing glutathione, comprising the steps of cultivating said strain; and mixing the additive with one or more material selected from the cultivated strain, its dry product, its extract, its culture, its lysate, and glutathione derived therefrom.

Another object of the present invention is to provide a composition for antioxidant action, detoxification, immunity enhancement, cosmetics, food or feed containing one or more selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

Another object of the present invention is to provide a composition for the preparation of a medical product used to prevent or treat a disease caused by glutathione deficiency, or a pharmaceutical composition for the prevention or treatment of a disease caused by glutathione deficiency, containing one or more than one selected from a strain ; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

Useful effects of the invention

The strain of the present invention can produce significantly more glutathione compared to existing glutathione producing strains. Therefore, the strain of the present invention can be effectively used to produce glutathione.

In addition, a composition containing one or more than one selected from the strain of the present invention; dry product, extract, culture and lysate of the specified strain; and glutathione, isolated from any one or more of said strain, dry product, extract, culture and lysate, has an exceptional ability to utilize oxygen radicals, and thus the composition can be effectively used as a composition for antioxidant action, detoxification or strengthening immunity.

Thus, the composition can also be effectively used as a cosmetic composition, medical composition and its preparation.

In addition, when the composition is used in foodstuffs, it improves the functions and properties of seasonings described above, and thus the composition can be usefully used to prepare a food composition and a feed composition.

BRIEF DESCRIPTION OF GRAPHICS

In FIG. 1 shows the results of sensory evaluation of food compositions each containing an extract of Saccharomyces cerevisiae strain CEN.PK2-1D, CJ-5 or CJ-37;

in fig. 2 shows the results of confirmation of the ability of extracts of strains of Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 to utilize radicals; And

in fig. 3 shows the results of confirmation of the ability of strains of Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 to scavenge oxygen radicals.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention will be described in detail below. At the same time, each description and embodiment disclosed in the present invention can also be applied to other descriptions and embodiments. That is, all combinations of the various elements disclosed in the present invention are within the scope of the present invention. In addition, the scope of the present invention is not limited to the specific description described below.

In addition, those skilled in the art will understand, or be able to ascertain through no more than routine experimentation, many equivalents to the specific embodiments of the present invention described herein. In addition, these equivalents should be interpreted as being included in the scope of the present invention.

According to one aspect of the present invention, a new glutathione producing strain of Saccharomyces cerevisiae can be provided.

The glutathione-producing strain of the present invention may be the Saccharomyces cerevisiae CJ-5 strain with accession number KSCM12568P.

The glutathione-producing strain of the present invention may contain a 18S rRNA (ribosomal ribonucleic acid) (ITS (internal transcribed spacer), 5.8S) sequence having a homology or identity of 90% or higher, in particular 91%, 92%, 93%, 93 .2% or higher, 93.5% or higher, or 93.7% or higher with the 18S rRNA sequence (ITS, 5.8S) of another strain of Saccharomyces cerevisiae belonging to the same species of the same genus, in particular a strain of Saccharomyces cerevisiae YJM1592 Saccharomyces cerevisiae (SEQ ID NO: 3), but the strain is not limited to this. More specifically, the glutathione-producing strain of the present invention may contain a 18S rRNA sequence (ITS, 5,8S) having a homology or identity of 90% or more, in particular 91% or more, 92% or more, 93% or more, 93, 2% or greater, 93.5% or greater, or 93.7% or greater, and less than 96%, less than 95.5%, or less than 95.2% identity with the 18S rRNA sequence (ITS, 5.8S) of strain YJM1592 , but the strain is not limited to this. The 18S rRNA sequence (ITS, 5.8S) is an internal transcribed spacer (ITS) sequence that exists between 18S and 5.8S rRNA and is the sequence used for species classification.

The glutathione-producing strain of the present invention may have a 18S rRNA (ITS, 5.8S) sequence of SEQ ID NO: 1. in particular 95% or higher, 96% or higher, 97% or higher, 98% or higher, or 99% or higher with SEQ ID NO: 1, but the 18S rRNA (ITS, 5,8S) sequence of the strain is not limited to this.

The term "homology" or "identity" as used herein refers to the degree of correspondence between two given amino acid sequences or nucleotide sequences, and may be expressed as a percentage. The terms "homology" and "identity" can often be used interchangeably.

Homology or sequence identity of a conserved polynucleotide or polypeptide can be determined using standard alignment algorithms, and the default gap penalties set by the program being used can be used together. Substantially homologous or identical sequences can hybridize under moderately or very stringent conditions over the entire length of the sequence, or at least over about 50%, 60%, 70%, 80%, or 90% of the entire length of the sequence. Additionally, consider polynucleotides that contain degenerate codons instead of codons when hybridized.

Whether any two polynucleotide or polypeptide sequences share homology, similarity, or identity can be determined using known computer algorithms such as the "FASTA" program, using default parameter values as described in Pearson et al, (1988) (Proc. Natl Acad. Sci. USA 85: 2444), or determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol Biol. 48: 443-453), which is implemented in the Needleman program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277) (version 5.0.0 or later) (including GCG software package (Devereux, J. et al., Nucleic Acids Research 12: 387 (1984)), BLASTP, BLASTN, FASTA (Atschul, S. F. et al., J. Mol. Biol. 215: 403 (1990); Guide to Huge Computers, Martin J. Bishop, ed., Academic Press, San Diego , 1994, and Carillo et al (1988) SIAM J. Applied Math 48: 1073) For example, the BLAST algorithm from the National Center for Biotechnology Information database or the ClustalW algorithm can be used to determine homology, similarity, or identity.

Homology, similarity, or identity of polynucleotides or polypeptides can be determined, for example, by comparing sequence information using a GAP computer program, such as Needleman et a.l. (1970), J. Mol. Biol. 48:443 as disclosed in Smith and Waterman, Adv. Appl. Math (1981) 2: 482. Briefly, the GAP program defines similarity as the number of similar aligned characters (ie, nucleotides or amino acids) divided by the total number of characters in the shorter of the two sequences. The default parameters for the GAP program may include: (1) a binary comparison matrix (containing a value of 1 for identity and 0 for no identity) and a weighted comparison matrix from Gribskov et al. (1986) Nucl. Acids Res. 14:6745 as disclosed in Schwartz and Dayhoff, eds., Atlas Of Protein Sequence And Structure, National Biomedical Research Foundation, pp.353-358 (1979) (or EDNAFULL substitution matrix (EMBOSS version NCBI NUC4.4)); (2) a penalty of 3.0 per gap and an additional penalty of 0.10 per symbol in each gap (or a penalty for opening a gap of 10, a penalty for extending a gap of 0.5); and (3) no penalty for end gaps.

Furthermore, whether any two polynucleotide or polypeptide sequences share homology, similarity, or identity can be established by comparing sequences in Southern hybridization experiments under certain stringent conditions, and certain appropriate hybridization conditions can be within the technical field and can be determined by in a manner well known to those skilled in the art.

The term "glutathione" of the present invention, which may be used interchangeably with "GSH", refers to a tripeptide composed of three amino acids: glutamate, cysteine and glycine. Glutathione can be used as a raw material for pharmaceuticals, functional health foods, flavor ingredients, foods, feed additives, cosmetics, and so on, but is not limited to.

The new strain of Saccharomyces cerevisiae of the present invention is characterized by a high ability to produce glutathione.

The strain may be a strain having an enhanced ability to produce glutathione, a strain having a high ability to produce glutathione, or a strain having an increased ability to produce glutathione.

The term "glutathione-producing strain" of the present invention can be used interchangeably with the terms "glutathione-producing strain", "glutathione-producing strain", and so on.

The strain of Saccharomyces cerevisiae of the present invention may contain glutathione in an amount of 0.6% by mass or more, in particular 0.7% by mass or more, 0.8% by mass or more, 0.9% by mass or more, 1 0.0% by mass or more, or 1.1% by mass or more, based on the dry weight of the strain, but the amount of glutathione contained in the strain is not limited to this.

According to another aspect of the present invention, there may be provided a composition for producing glutathione comprising said strain.

In the present invention, "glutathione production" or "glutathione production" may include accumulation of glutathione in the strain.

The composition for producing glutathione may be a composition for producing glutathione with the strain of the present invention, and for example, the composition may contain a strain and may also contain an additional composition for producing glutathione using the strain, without limitation.

The composition for producing glutathione of the present invention may further comprise any suitable additive commonly used in the composition for producing glutathione. The supplement may be a supplement of natural or non-natural origin, but is not limited to this.

The additive may include an excipient and/or an emulsifier. The excipient may be suitably selected for use according to the intended use or form and may be, for example, one or more selected from starch, glucose, cellulose, lactose, glycogen, D-mannitol, sorbitol, lactitol, maltodextrin, calcium carbonate, synthetic aluminosilicate, calcium monohydrogen phosphate, calcium sulfate, sodium chloride, sodium bicarbonate, purified lanolin, dextrin, sodium alginate, methylcellulose, colloidal silica gel, hydroxypropyl starch, hydroxypropyl methylcellulose,

propylene glycol, casein, calcium lactate, primogel, gum arabic and, in particular, one or more selected from starch, glucose, cellulose, lactose, dextrin, glycogen, D-mannitol and maltodextrin, but the excipient is not limited to them. The emulsifier may be a glycerol ester, sorbitan ester, monoglyceride, diglyceride, triglyceride, sucrose ester, sorbitan ester, propylene glycol ester, glycerol fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester , a sucrose fatty acid ester, lecithin, or a mixture thereof, but the emulsifier is not limited to them, and emulsifiers known in the art may be appropriately used.

The excipient may include, for example, a preservative, a wetting agent, a dispersing agent, a suspending agent, a buffering agent, a stabilizing agent, or an isotonic agent, but the excipient is not limited to them.

According to another aspect of the present invention, a method for producing glutathione can be provided, comprising the step of cultivating said strain. During the cultivation of the strain, glutathione can accumulate in the strain.

The media and other culture conditions used for culturing the strain of the present invention can be used without particular restrictions, since they are usually used for culturing microorganisms of the genus Saccharomyces. In particular, the strain of the present invention can be cultivated in a conventional medium containing suitable carbon sources, nitrogen sources, phosphorus sources, inorganic compounds, amino acids and/or vitamins, under controlled temperature, pH, etc. under aerobic or anaerobic conditions.

In the present invention, carbon sources may include carbohydrates such as glucose, fructose, sucrose, maltose, and so on; sugar alcohols such as mannitol, sorbitol and so on; organic acids such as pyruvate, lactate, citrate and so on; and amino acids such as glutamic acid, methionine, lysine and so on, but carbon sources are not limited to them. In addition, natural organic nutrients such as starch hydrolyzate, molasses, molasses, rice bran, cassava, cane molasses and corn extract can be used, and carbohydrates such as glucose and sterile pre-treated molasses (i.e. molasses, converted to reducing sugar) and so on. In addition, various other carbon sources can be used in a suitable amount without limitation. These carbon sources may be used alone or in combination with, but are not limited to, two or more of them.

Nitrogen sources may include inorganic nitrogen sources such as ammonia, ammonium sulfate, ammonium chloride, ammonium acetate, ammonium phosphate, ammonium carbonate, ammonium nitrate, and so on; and organic nitrogen sources such as amino acids, peptone, NZ-amine, meat extract, yeast extract, malt extract, corn extract, casein hydrolyzate, fish or its decomposition products, defatted soybean meal or its decomposition products, and so on. These nitrogen sources may be used alone or in combination with, but are not limited to, two or more of them.

Phosphorus sources may include potassium dihydrogen phosphate, dipotassium phosphate, the corresponding sodium salts, and so on. Inorganic compounds may include sodium chloride, calcium chloride, ferrous chloride, magnesium sulfate, ferrous sulfate, manganese sulfate, calcium carbonate, and so on.

In addition, amino acids, vitamins and/or suitable precursors may be included in the above medium. L-amino acids and so on can be added to the strain culture medium. In particular, glycine, glutamate and/or cysteine may be added, and, if necessary, L-amino acids such as lysine may be further added, but they are not necessarily limited to them.

These media or precursors may be added to the culture in batch or continuous mode, but are not limited to this.

In the present invention, the pH of the culture can be adjusted during the cultivation of the strain by adding a compound such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid and sulfuric acid to the culture, as appropriate. In addition, a defoamer such as a fatty acid polyglycol ester can be used during culturing to suppress foaming. In addition, oxygen or an oxygen-containing gas can be introduced into the culture to maintain the culture under aerobic conditions; or nitrogen, hydrogen, or carbon dioxide gas may be introduced, or no gas may be introduced at all to maintain anaerobic or microaerobic conditions.

The temperature of the culture may be from 25°C to 40°C, and more specifically, from 28°C to 37°C, but is not limited to this. Cultivation can be continued until the desired amount of useful substances is obtained, in particular, within 1-100 hours, but is not limited to this.

The method for producing glutathione may further include an additional process after the cultivation step. The additional process may be appropriately selected according to the application of glutathione.

In particular, the method for producing glutathione may further include the step of separating glutathione from one or more than one material selected from the strain, its dry product, its extract, its culture, and its lysate, after the cultivation step.

As used herein, the term "culture" may refer to the product resulting from the cultivation of the strain of the present invention. For example, the culture may be a medium containing by-products that are formed as a result of nutrient intake and metabolism during the cultivation of the strain in the medium, and the culture may include all types of cultures that can be obtained using the medium, such as dilute or concentrated culture a liquid, a dry product obtained by drying a medium, a crude purified product or a purified medium product, or a mixture thereof, and so on. The culture of the microorganism of the present invention may or may not include the microorganism. The cultivation is as described above.

As used herein, the term "lysate" may refer to the product resulting from the disruption or lysis of the strain or culture thereof, or to the supernatant obtained by centrifugation of the lysate.

The culture, the lysate, its supernatant and its fractions are also included within the scope of the present invention. The method may further include the step of lysing the strain prior to or simultaneously with the isolation step. Lysis of the strain can be carried out by a method commonly used in the technical field to which the present invention relates, for example, using a lysis buffer solution, an ultrasonicator, heat treatment, a French press, and so on. In addition, the lysis step may include the action of enzymes such as a cell wall degrading enzyme, nuclease, nucleotidyl transferase, protease, and so on, but the lysis step is not limited to this.

In view of the objects of the present invention, yeast powder, yeast extract, yeast extract powder mixture or pure glutathione having a high glutathione content can be obtained by the glutathione production method, but the products that can be obtained in this way are not limited to them, and these products can be suitably prepared according to the required product.

As used herein, the term "dry yeast" may be used interchangeably with the term "dry strain product" and so on. Dry yeast can be obtained by drying a yeast strain in which glutathione is accumulated, and in particular, can be included in a feed composition, food composition, and so on, but the dry yeast production method is not limited to this.

As used herein, the term "yeast extract" may be used interchangeably with the term "strain extract" and so on. The term "strain extract" may refer to the substance that remains after separation of the cell wall from the protoplast of the strain. In particular, the extract of the strain may refer to the remaining components, with the exception of the cell wall, obtained by lysis of yeast cells. The extract of the strain may include glutathione and may include, as components other than glutathione, one or more components of proteins, carbohydrates, nucleic acids, and fibers, but the components that may be included are not limited to them.

The isolation step can be carried out using a suitable method known in the art to thereby isolate glutathione, which is the desired substance.

The isolation step may include a purification process. The purification process may be a process of isolating only pure glutathione from the strain. Through a purification process, pure glutathione can be obtained.

If necessary, the method for producing glutathione may further include the step of mixing the additive with a material selected from the strain obtained after the cultivation step, its dry product, extract, culture and lysate, and glutathione isolated therefrom. In this mixing step, a powdered yeast extract mixture can be obtained.

The additive may include an excipient and/or an emulsifier. The excipient and emulsifier may be suitably selected for use by those skilled in the art, and examples thereof are as described above.

According to another aspect of the present invention, a method for preparing a composition containing glutathione can be provided, comprising the steps of cultivating a strain; and mixing the additive with a material selected from the cultivated strain, its dry product, extract, culture and lysate, and glutathione isolated therefrom.

The composition of the present invention may further contain a substance of natural origin or a substance of non-natural origin. Depending on the intended use of the composition, the substance may include, but is not limited to, a pharmaceutically acceptable carrier, a sitologically acceptable carrier, a cosmetically acceptable carrier, and so on. The carrier may be suitably selected by those skilled in the art based on known composition.

In the composition, the content of the material selected from the strain of the present invention, its dry product, extract, culture and lysate, and glutathione isolated from them, can be from 3% by weight to 30% by weight, based on the total weight of the composition.

The additive may include an excipient and/or an emulsifier. The excipient and emulsifier may be suitably selected for use by those skilled in the art, and examples thereof are as described above.

According to another aspect of the present invention, there may be provided a composition for antioxidant action, detoxification, immunity enhancement, cosmetics, food or feed, containing one or more than one selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

According to another aspect of the present invention, there may be provided a method for preparing an antioxidant, detox, immune, cosmetic, food or feed composition comprising one or more selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

In view of the objectives of the present invention, the composition may contain the strain itself, or may contain a culture of the strain, a dry product of the strain, an extract of the strain or a lysate of the strain, or may contain glutathione isolated from such a culture, extract, dry product or lysate of the strain, but without limitation them. In other words, since the strain, its dry product, extract, culture or lysate contains glutathione, glutathione can be included in any of these forms without limitation, as long as the glutathione content is not increased to the required level, although the type may differ depending on specific application of the composition.

In the composition, the content of the material selected from the strain of the present invention, its dry product, extract, culture and lysate, and glutathione isolated from them, can be from 3% by weight to 30% by weight, based on the total weight of the composition. In addition, the composition may further contain any suitable additive commonly used in compositions.

The composition may have an increased content of glutathione due to the inclusion of one or more than one material selected from the glutathione-producing strain of the present invention; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

According to one aspect of the present invention, there may be provided a composition for antioxidant action comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

As used herein, the term "antioxidant" may broadly refer to the action of inhibiting, reducing, or controlling natural oxidation reactions, and more specifically, the action of inhibiting, reducing, or controlling the formation or exposure of free radicals produced in the body, hydrogen peroxide, or peroxides, formed from free radicals, or hydroxyl radicals formed from hydrogen peroxide.

According to one aspect of the present invention, a detoxification composition may be provided comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

As used herein, the term "detoxification" may refer to all activities to eliminate or reduce toxicity caused by harmful substances.

Glutathione, which is an endogenous antioxidant produced by cells, not only directly participates in the neutralization of free radicals and oxygen radical compounds, but also maintains exogenous antioxidants such as vitamins C and E in a reduced form, and thus detoxification may include actions to inhibit , reducing or controlling the formation or exposure to oxygen radicals and free radicals, hydrogen peroxide or peroxides generated from free radicals, or hydroxyl radicals generated from hydrogen peroxide. In addition, since glutathione can protect the thiol groups of cellular proteins, it can prevent side effects caused by overdose of drugs such as acetaminophen and so on, and is also used to detoxify methylglyoxal, which is formed as a metabolic by-product. Therefore, reducing or reducing the toxicity of the compounds may also be included within the scope of the term "detoxification".

According to one aspect of the present invention, an immune enhancing composition can be provided comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

As used herein, the term "enhancing immunity" refers to enhancing the body's defense against antigens, and in particular, may refer to an increase in cellular and humoral immunity against antigens, and in particular, may refer to increased immunity compared to immunity before administration of the composition. The immunity enhancement mechanism is not limited, but may include, for example, immunity enhancement achieved by stimulating the activity of antigen-presenting cells such as macrophages and so on, or stimulating the specific activity of lymphocytes.

As described above, glutathione has a strong antioxidant capacity, plays a role in improving liver functions, and promotes the breakdown of toxic substances in the body, and eliminates harmful oxygen radicals, and participates in the immune system. That is, glutathione increases the activity of T-lymphocytes and leukocytes to improve the body's immunity and restore the strength of people weakened due to various chronic diseases, and so on. Thus, one or more than one material selected from the glutathione-containing strain of the present invention; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of the above strain, dry product, extract, culture and lysate can be effectively used to enhance immunity.

According to one aspect of the present invention, there may be provided a cosmetic composition comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

The "composition for cosmetics" of the present invention may be formulated as a composition selected from the group consisting of solution, topical ointment, cream, foam, nourishing emollient, light emollient, mask, softening water, milk, foundation. makeup, essence, liquid cleanser, bath, sunscreen, suntan oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant cleanser, oil, foundation powder, emulsion base, wax base, patch and spray, but not limited to.

The cosmetic composition may further comprise one or more cosmetically acceptable carriers which are admixed with conventional skin cosmetics and may be appropriately admixed with conventional ingredients e.g. oil, water, surfactant, humectant, lower alcohol , thickener, chelating agent, pigment, preservative, fragrance, and so on, but cosmetically acceptable carriers are not limited to them. The cosmetically acceptable carrier included in the cosmetic composition of the present invention may be appropriately selected by those skilled in the art in accordance with the composition of the cosmetic composition.

According to one aspect of the present invention, a food composition may be provided comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

In one specific embodiment, the food composition may include a protoplast or an extract of the strain of the present invention and, more specifically, may be prepared for use in a suitable composition by mixing the strain extract with an additive in accordance with, but not limited to, the food product used. The strain extract is as described above.

The additive may include an excipient and/or an emulsifier. The excipient and emulsifier may be suitably selected for use by those skilled in the art, and examples thereof are as described above. In addition, an auxiliary food additive, a food stabilizer, a water-retaining agent, and so on can be included.

The "food composition" of the present invention may include all types of functional foods, dietary supplements, dietary foods and nutritional supplements.

The above types of food composition can be prepared in various forms in accordance with the usual method known in the art.

The food composition may be formulated as pills, powders, granules, infusions, tablets, capsules, liquids, and so on. Food products to which the composition of the present invention may be added may include, for example, various food products such as rice, edible ground grain (edible cereal flour), cereal soups, rice bowl with toppings, noodles, rice soups, instant rice, condiments, packaged cooked rice, dry boiled rice, bread, edible sugars, rice cakes, sauce mixes, sauces, spices, food salt, spices, spice powders, processed, frozen, dried and cooked fruits and vegetables , jellies, jams, candied fruits, eggs, milk and other dairy products, edible fats and oils, coffee, cocoa and coffee substitutes, tapioca, cereal flour and cereal products, ramen, udon, noodles, chopped noodles, cold noodles, porridge, soups , soup dishes, instant food, frozen food, food in heat-resistant bags, other drinks, chewing gums, teas, vitamin complexes, nutritional supplements, and so on, but the forms of food products are not limited to them.

As an ingredient that can be included in the food composition of the present invention, additional ingredients such as various plant extracts, food additives, or natural carbohydrates, as in conventional foods, can be included. In addition, the auxiliary food additive may include auxiliary food additives generally accepted in the art, such as flavoring agents, flavor enhancers, colorants, fillers, stabilizers, and so on.

Examples of natural carbohydrate include monosaccharides such as glucose, fructose, and so on; disaccharides such as maltose, sucrose and so on; and polysaccharides such as common sugars such as dextrin, cyclodextrin and so on, and sugar alcohols such as xylitol, sorbitol, erythritol and so on. In addition to those described above, natural flavors (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be successfully used as flavors. In addition, conventional food additives used to improve taste and nutrition, such as nucleic acids, amino acids, organic acids, and so on, can be added.

In addition to the compositions described above, the food composition of the present invention may include a variety of dietary supplements, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colors and fillers (cheese, chocolate, etc.) , pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonizing agents used in carbonated beverages, and so on. In addition, the composition may include fruit pulp for the preparation of natural fruit juices, fruit juice drinks and vegetable drinks. These ingredients may be used singly or in combination.

The food products of the present invention may be prepared by a method commonly used in the art, and raw materials and ingredients commonly added in the art for preparation may be added during preparation. In addition, food products may be present in any composition without limitation if such composition is considered a food product.

In addition, when the food composition of the present invention is used as a functional health food, the food composition of the present invention can be prepared in various composition forms. The nutritional composition of the present invention has the advantage that it does not have side effects that may occur when drugs are administered for a long period of time, since the nutritional composition of the present invention is prepared using raw materials, unlike a conventional drug, and it has excellent tolerance. Therefore, the food product of the present invention can be used as a supplement.

Meanwhile, the food composition of the present invention may also include flavoring agents, flavor enhancers, coloring agents, fillers, stabilizers, and flavoring agents, which may also be classified as food additives.

The term "flavoring" as used herein may refer to a substance added to enhance the flavor of food products. The flavoring agent may be a substance that allows foodstuffs to have excellent seasoning properties.

Flavor can be classified according to taste components. In other words, the flavor can be classified as neutral flavor, beef flavor, chicken flavor, pork flavor, kokumi flavor, and so on, depending on the taste.

The term "kokumi flavor" refers to a flavor that releases a "kokumi" flavor. The term "kokumi" is a Japanese word and can also be expressed as "mouth fullness", "length", "thickness" and "heartiness" in English, and expressed as "rich taste", "thick taste", "rich taste" ”, “tight taste”, “obsessive taste” and so on in Korean. "Neutral flavor" refers to a flavor that maximizes "umami" and minimizes other flavors for a mild and clean flavor. For example, oils such as canola oil or grape seed oil among oils can be recognized as having a neutral aroma. The term "seasoning property" can mean, but is not limited to, having a sour, sweet, salty, bitter or umami taste.

The food composition of the present invention may contain from 3% by weight to 30% by weight of the yeast extract of the present invention, based on the total weight of the composition, and may further contain one or more selected from ammonium chloride, maltodextrin, crystalline glucose powder and an emulsifier. In a specific embodiment, the food composition may contain from 3% by weight to 25% by weight of the yeast extract of the present invention, from 20% by weight to 35% by weight of ammonium chloride, from 25% by weight to 35% by weight of maltodextrin, from 3 % by weight to 7% by weight of crystalline powdered glucose and from 0.1% by weight to 1% by weight of an emulsifier, based on the total weight of the composition, but the food composition is not limited to this.

According to one aspect of the present invention, a feed composition may be provided comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any of said strain, dry product, extract, culture, and lysate.

In particular, the feed composition may contain any one or more strains of the present invention as it is, a dry product of said strain and an extract of said strain, or may contain cell wall components obtained during the preparation of an extract of said strain and, for example, a dry product the specified strain (dry yeast) and/or extract of the specified strain (yeast extract), but is not limited to them.

The "feed composition" of the present invention is any suitable natural or artificial food, meal, etc., intended to be eaten, ingested and digested by animals, or components of a meal. A feed comprising the glutathione-enhanced yeast of the present invention may be prepared in various feed forms known in the art, and in particular concentrated feeds, raw feeds and/or specialty feeds may be included.

The type of food is not particularly limited, but any food commonly used in the art can be used. Non-limiting examples of foods may include plant-based foods such as grains, root vegetables, food by-products, seaweed, fibers, drug by-products, oils, starches, meals, grain processing by-products, and so on; and animal feeds such as proteins, inorganic substances, minerals, fats and oils, unicellular proteins, zooplankton, foodstuffs, and so on. These foods may be used alone or in combination of two or more of them.

According to another aspect of the present invention, there may be provided a composition for the preparation of a medical product used to prevent or treat a disease caused by glutathione deficiency, comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

According to another aspect of the present invention, there may be provided a pharmaceutical composition for preventing or treating a disease caused by glutathione deficiency, comprising one or more material selected from a strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate.

The term "pharmaceutical composition" as used herein may refer to a chemical/biological compound or substance, or a mixture or combination of two or more such compounds or substances, intended for use in medical diagnosis, therapy, treatment or prevention of a disease or pathology.

As used herein, the term "composition for the preparation of a medical product" may be used in the same sense as a pharmaceutical composition, or may refer to a composition that also includes any additional ingredients necessary for the preparation and/or incorporation into a medical product. However, the composition is not limited to this.

As described above, glutathione contributes to antioxidant action by decomposing and scavenging oxygen radicals, detoxifying action and boosting immunity. Thus, a strain or culture thereof, dry product, extract or lysate containing glutathione in high concentrations can be effectively used to obtain a medical product that can be effectively used to treat a disease caused by glutathione deficiency.

In addition, since the strain or its culture, dry product, extract or lysate contains glutathione in high concentrations, they can be included, as they are, in a pharmaceutical composition for the prevention or treatment of a disease caused by glutathione deficiency.

The disease is not limited as long as it is a glutathione deficiency disease. For example, the disease includes any disease, without limitation, if it is caused by the accumulation of oxygen radicals and/or reduced immunity and, in particular, atherosclerosis; neurodegenerative diseases including Lou Gehrig's disease, Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis and Huntington's disease; cardiovascular diseases, including myocardial infarction, angina pectoris, coronary heart disease and ischemic heart disease; cerebral ischemia, including stroke; digestive disorders, including diabetes, gastritis and stomach cancer; cancer; leukemia; cataract; aging; rheumatoid arthritis; hepatitis; atopic dermatitis; and so on, but the disease is not limited to them.

The medical and/or pharmaceutical composition may further comprise a pharmaceutically acceptable carrier. As used herein, the term "pharmaceutically acceptable" may mean the property of being non-toxic to cells or people exposed to a medical product. Any carrier can be used without limitation as long as it is known in the art, such as a buffering agent, a preservative, an analgesic, a solubilizer, an isotonic agent, a stabilizer, a general agent, an excipient, a lubricant, and so on.

In addition, the medical and/or pharmaceutical composition may be formulated into oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols; preparations for external use; suppositories; and sterile injectable solutions, by conventional means. In addition, they can be used as an external preparation for the skin, such as an ointment, lotion, spray, patch, cream, powder, suspension or gel. Carriers, excipients and diluents that may be included in the medical product of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum arabic, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oils. When preparing preparations, they are prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, and so on, which are commonly used.

Solid preparations for oral administration include tablets, pills, powders, granules and capsules, and such solid preparations can be prepared by mixing the composition with at least one excipient, for example starch, calcium carbonate, sucrose, lactose, gelatin, and so on. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspension, oral liquid, emulsion, syrup, and so on, and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agent, sweetener, flavoring agent may be included. , preservative and so on. Formulations for parenteral administration include a sterile aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a lyophilized agent, and a suppository. As the non-aqueous solvent and suspension, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and so on can be used. Witepsol, macrogol, tween 61, cocoa butter, lauric oil, glycerogelatin and so on can be used as the basis for suppositories.

The medical and/or pharmaceutical composition may be administered in a pharmaceutically effective amount. The term "administration" as used herein refers to the administration of a preselected substance to an individual via an appropriate method. The composition may be administered by any conventional route provided that it is capable of reaching the target tissue and the route of administration may include intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, intrapulmonary administration and rectal administration. administration, but the route of administration is not limited to this.

The term "individual" can refer to all animals, including humans, rats, mice, livestock, and so on. In particular, it may be a mammal, including a human.

The term "pharmaceutically effective amount" refers to an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to any medical treatment without side effects. The effective dose level can be readily determined by those skilled in the art depending on factors including patient gender, age, body weight, health status, disease type, severity, drug activity, drug susceptibility, route of administration, time of administration, route of administration. administration, rate of recovery, period of treatment, and drugs used in combination or simultaneously, and other factors well known in the medical field. The above recommended dose may be administered once a day or in several divided doses.

According to another aspect of the present invention, the use of the strain in the production of glutathione may be provided.

The strain is as described above.

EXAMPLES

Hereinafter, the present invention will be described in more detail with reference to examples and experimental examples. However, these examples and experimental examples are for illustrative purposes only, and the scope of the present invention is not intended to be limited by these examples and experimental examples.

Example 1 Selection of a Strain with Exceptional Glutathione Producing Ability and Verification of Glutathione Producing Ability

The strains were isolated from Korean traditional Nuruk containing various strains, and these strains were used to select a strain having a high ability to produce glutathione.

More specifically, samples of grains such as rice, barley, mung bean, oats, and so on, were collected from a total of 20 districts, including Yongin, Icheon, Pyeongtaek, Hwaseong, and so on, in Gyeonggi Province, Korea, and were cooked korean traditional nuruk. The collected grain samples were crushed, kneaded, wrapped in cloth, tightly pressed into shape, wrapped in straw and fermented for 10 days, and then slowly dried to prepare traditional Korean nuruk. The following experiments were carried out to isolate different strains from cooked Korean traditional nuruk. 45 ml of saline was added to 5 g of Korean traditional nuruk and ground with a mixer. To isolate pure yeast strains, serial dilutions were performed, and the resulting final dilution was plated on the surface of YPD agar (10 g/L yeast extract, 20 g/L bactopeptone, 20 g/L glucose per 1 L of distilled water) and cultivated at 30°C. C within 48 hours. After studying the morphology of the colonies and checking under a microscope, each yeast colony was streaked onto YPD agar. 25 ml of YPD broth was placed in a 250 ml Erlenmeyer flask and inoculated with pure isolated strains, and shaking culture (30° C., 200 rpm) was performed for 48 hours to study the amount of glutathione produced, followed by strain screening.

To improve the originally isolated strains, a random mutation was induced in each isolated strain. Among yeast strains isolated from traditional Korean nuruk, a strain was selected that has the ability to produce 15 mg/l of glutathione and was named strain CJ-37. The CJ-37 strain was cultured on a solid medium and then inoculated into a broth to obtain a culture. Yeast cells were irradiated with UV radiation using a UV lamp. After that, the culture liquid irradiated with UV radiation was sown on a dish with the medium, and only the mutant strains that formed colonies were isolated, and the amount of glutathione produced by these mutant strains was studied.

As a result, it was confirmed that the production of glutathione by these mutant strains was up to 105 mg/L, and the strain showing the highest amount of glutathione produced (105 mg/L) was selected as the glutathione-producing strain and was named CJ-5 strain, which was deposited in accordance with the Budapest Treaty with the International Depository Authority, Korea Culture Center for Microorganisms (KCCM), on July 31, 2019 under registration number KCCM12568P.

Example 2 Comparison of Glutathione Producing Ability In order to confirm the ability of the CJ-5 strain selected in Example 1 to produce glutathione, three kinds of known microorganisms (Table 1) were cultured by the following method, and then compared and analyzed the ability to produce GSH. As a control, the CJ-37 strain obtained in Example 1 was also cultured and analyzed for comparison.

Each strain was first cultured at 30°C for 1 day and then inoculated into a 250 ml baffled flask containing 25 ml YPD medium (10 g/l yeast extract, 20 g/l bactopeptone, 20 g/l glucose per 1 liter of distilled water). Amino acids (L-cysteine, L-glutamic acid and L-glycine) were added at a concentration of 20 mM after 16 hours from the start of cultivation and cultured at 30°C at 200 rpm.

To measure GSH concentrations, 500 μl of the culture sample containing the cells was lysed at 80° C. at 800 rpm for 10 minutes, and then the GSH concentrations were measured using the GSH Ration Detection Assay (Abcam). Dry weight was calculated by converting OD (optical density) values and used to calculate GSH content (%)=GSH weight/yeast dry weight. The results are presented in table 2.

As a result of evaluating the ability of 5 types of yeast to produce GSH, it was confirmed that the CJ-5 strain has the highest content of GSH in terms of cell weight. In particular, strain CJ-5 was confirmed to have the highest GSH content of about 200% to about 300% based on yeast cell weight compared to existing GSH-producing strains (Candida utilis strain KCCM50667, Kluyveromyces lactis strain ATCC8585 and Saccharomyces cerevisiae CEN.PK2-1D), and in particular, the ability of strain CJ-5 to produce GSH was improved by approximately 584% compared to CJ-37.

These results confirmed that the CJ-5 strain has an exceptional ability to produce GSH, which suggests that the strains of the present invention can be effectively used for the production of GSH.

Example 3: Sequence Verification of 18S rRNA (ITS, 5,8S) of CJ-5 Strain by Gene Sequencing

To confirm the classification of the highest glutathione-producing capacity CJ-5 strain and the lowest glutathione-producing capacity CJ-37 strain, which were isolated in Example 1, the 18S rRNA (ITS, 5.8S) sequences of the two were compared and analyzed. strains.

More specifically, sequence similarity in 18S ribosomal RNA (rRNA) (ITS, 5.8S) between the two strains was compared using the BLAST database (http://www.ncbi.nlm.nih.gob/blast/). The 18S rRNA sequence (ITS, 5.8S) of CJ-5 strain is SEQ ID NO: 1, the 18S rRNA sequence (ITS, 5.8S) of CJ-37 strain is SEQ ID NO: 2, and the 18S rRNA sequence (ITS , 5,8S) Saccharomyces cerevisiae YJM1592 chromosome XII is SEQ ID NO: 3.

As a result, the CJ-5 strain showed approximately 93.73% similarity to the 18S rRNA sequence (ITS, 5.8S) of chromosome XII of Saccharomyces cerevisiae YJM1592. At the same time, the CJ-37 strain also showed approximately 95.20% similarity to the 18S rRNA sequence (ITS, 5.8S) of chromosome XII of Saccharomyces cerevisiae YJM1592.

These results show that both strain CJ-5 and strain CJ-37 are strains of Saccharomyces cerevisiae. These results, in conjunction with the experimental results of Example 2, can be interpreted in such a way that the CJ-5 strain of the present invention has the ability to produce GSH approximately 6 times greater than that of a microorganism belonging to the same species of the same genus, as well as a microorganism belonging to to another species of a different genus, and also has the ability to produce 284% (i.e., approximately 3 times) more GSH compared to Saccharomyces cerevisiae CEN.PK2-1D, which is another microorganism belonging to the same species of the same genus.

These results suggest that the strain of the present invention has a high ability to produce GSH, and thus the strain, its dry product, extract, culture and/or lysate can be used in various cosmetics, foods and feeds prepared using GSH as starting material.

Example 4 Preparation of a composition containing glutathione To obtain a yeast extract containing glutathione, yeast cultures of Saccharomyces cerevisiae CEN.PK2-1D, and CJ-5, and CJ-37 were first centrifuged using a centrifuge.

Each of the centrifuged yeast samples was washed twice and subjected to repeated centrifugation to obtain each yeast sample. Thereafter, a cell wall degrading enzyme, a nuclease, a nucleotidyl transferase, and a protease were added, and an enzymatic reaction was carried out at pH 5.2 and a temperature of 45° C. for 48 hours.

After the enzymatic reaction, the enzymes in the supernatant were inactivated by heat treatment at 85° C. for 30 minutes, and each enzyme-inactivated supernatant was concentrated to obtain a yeast extract.

As a result, extracts of the yeast Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 were shown to contain glutathione at approximately 0.8%, 2.2% and 0.4% based on total weight.

Example 5 Sensory Evaluation of a Food Composition Containing Glutathione

Food compositions containing yeast extracts prepared in example 4 were subjected to organoleptic evaluation. More specifically, for Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 yeast extracts, organoleptic evaluation was performed using consommé chicken soup as a base.

Organoleptic evaluation was performed for tasters using a consommé chicken soup mixture containing 5% of each of the yeast extracts. Evaluations were made for persistence (“kokumi”), pleasant savory taste (“umami”), bitter taste (off-flavour), chicken flavor enhancement, mild and deep flavor, and overall preference.

As a result, CJ-5 yeast extract was confirmed to have excellent flavor retention ("kokumi") and a milder and deeper flavor compared to CEN.PK2-1D yeast extract and CJ-37 yeast extract, and the general preference for CJ- 5 was elevated (FIG. 1).

These results confirmed that the yeast extract of the present invention can be effectively used to prepare a food composition having excellent seasoning properties, and thus one or more selected from a yeast strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of these strains, dry product, extract, culture and lysate, can also be effectively used for the preparation of food compositions.

Example 6 Testing the Antioxidant Effect of a Composition Containing Glutathione

Example 6-1: Radical scavenging capability study

The ability of yeast extracts containing glutathione to utilize radicals was evaluated.

More specifically, 5 ml of 0.1 mM 1,1-diphenyl-2-picrylhydrazyl was added to 5 ml of each suspension of Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 yeast extracts prepared in Example 4 and left to interaction for 30 minutes in a dark place. Then the optical density was measured at 517 nm. In the concentration range from 2 mg/mL to 10 mg/mL, extracts of CEN.PK2-1D, CJ-5 and CJ-37 demonstrated the ability to utilize radicals from 21% to 61%, from 40% to 83% and from 12% to 49%. %, respectively (figure 2). At a concentration of 10 mg/ml, the antioxidant activity of CJ-5 yeast extract with a high content of glutathione was increased up to 1.7 times compared to the activity of CEN.PK2-1D and CJ-37 yeast extracts.

Example 6-2 Oxygen Radical Scavenging Test

To evaluate the antioxidant activity of yeast extracts containing glutathione, oxygen radical scavenging capacity (ORAC) was analyzed.

More specifically, 5 ml of each suspension of Saccharomyces cerevisiae CEN.PK2-1D, CJ-5 and CJ-37 yeast extracts prepared in Example 4 was mixed with 40 ml of fluorescein and then allowed to react at 37°C for 15 minutes. 25 ml of 2,2'-azobis-(2-amidinopropane) hydrochloride was added thereto, and the absorbance was measured at 485 nm and 528 nm for 60 minutes. The ability to absorb oxygen radicals was compared by obtaining the area under the curve (AUC). The ability of the CJ-5 yeast extract to scavenge oxygen radicals was higher than that of the CEN.PK2-1D yeast extract and the CJ-37 yeast extract (Fig. 3). As described above, the CJ-5 yeast extract of the present invention showed significantly higher antioxidant activity compared to the CEN.PK2-1D and CJ-37 yeast extracts.

These results confirmed that the yeast extract exhibited excellent antioxidant activity and thus one or more selected from the yeast strain; dry product, extract, culture and lysate of the specified strain; and glutathione isolated from any one or more of said strain, dry product, extract, culture, and lysate can also be effectively used for antioxidant protection, detoxification, and immune enhancement.

Preparation Example 1: Preparation of a Food Composition Containing Yeast Extract

Through the above experimental examples, the unique umami taste and off-flavour suppression effect of the yeast extract was confirmed, and thus the food composition containing the yeast extract of the present invention was prepared.

More specifically, a yeast extract powder mixture was prepared with a relative content of 3% to 30% yeast extract, 20% to 40% ammonium chloride, 20% to 40% maltodextrin, 1% to 10% crystalline glucose powder, and 0. 1% to 1% emulsifier.

Based on the above description, those skilled in the art will appreciate that the present invention may be embodied in other specific forms without changing its technical nature or essential characteristics. Therefore, it should be understood that the above embodiment is not restrictive, but illustrative in all aspects. The scope of the invention is defined by the appended claims and not by the description that precedes it, and, therefore, all changes and modifications falling within the limits and limits of the claims, or equivalents of such limits and limits, as intended on this basis, are covered by the claims.

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SEQUENCE LIST

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GLUTATHIONE WITH ITS USE

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<---

Claims (17)

1. Glutathione-producing Saccharomyces cerevisiae accession number KCCM12568P. 2. The strain according to claim 1, containing the nucleotide sequence of SEQ ID NO: 1 as the sequence of 18S rRNA. 3. A method for producing glutathione, including the stage of cultivating a strain according to claim 1. 4. The method of claim 3, further comprising the step of isolating glutathione from one or more material selected from the cultivated strain, a dry product of said strain, an extract of said strain, a culture of said strain, and a lysate of said strain. 5. A method for preparing a composition containing glutathione, comprising the steps: cultivating the strain according to claim 1 and mixing the additive with one or more than one material selected from the cultivated strain, the dry product of the said strain, the extract of the said strain, the culture of the said strain, the lysate of the said strain and the glutathione obtained therefrom. 6. The method of claim 5 wherein the additive comprises an excipient or emulsifier. 7. The method according to claim 5, where the amount of one or more than one material selected from the cultivated strain, the dry product of the specified strain, the extract of the specified strain, the culture of the specified strain, the lysate of the specified strain and the glutathione obtained from them is from 3% by weight up to 30% by weight, based on the total weight of the composition. 8. Composition for antioxidant action, containing one or more than one material selected from the strain according to claim 1, the dry product of the specified strain, the extract of the specified strain, the culture of the specified strain and the lysate of the specified strain and glutathione isolated from any one or more than one from said strain, dry product, extract, culture and lysate, and a carrier. 9. A detoxification composition comprising one or more material selected from a strain according to claim 1, a dry product of said strain, an extract of said strain, a culture of said strain and a lysate of said strain, and glutathione isolated from any one or more than one of said strain, dry product, extract, culture and lysate, and carrier. 10. An immune enhancing composition comprising one or more material selected from a strain of claim 1, a dry product of said strain, an extract of said strain, a culture of said strain and a lysate of said strain, and glutathione isolated from any one or more than one from said strain, dry product, extract, culture and lysate, and a carrier. 11. A composition for cosmetics containing one or more than one material selected from a strain according to claim 1, a dry product of said strain, an extract of said strain, a culture of said strain and a lysate of said strain, and glutathione isolated from any one or more than one from said strain, dry product, extract, culture and lysate, and a carrier. 12. A food composition containing one or more than one material selected from a strain according to claim 1, a dry product of said strain, an extract of said strain, a culture of said strain and a lysate of said strain, and glutathione isolated from any one or more than one from said strain, dry product, extract, culture and lysate, and a carrier. 13. A feed composition containing one or more material selected from a strain according to claim 1, a dry product of said strain, an extract of said strain, a culture of said strain and a lysate of said strain, and glutathione isolated from any one or more than one of said strain, dry product, extract, culture and lysate, and carrier. 14. Composition for obtaining a medical product used to prevent or treat a disease caused by glutathione deficiency, containing one or more than one material selected from the strain according to claim 1, a dry product of the specified strain, an extract of the specified strain, a culture of the specified strain and a lysate of the specified strain and glutathione isolated from any one or more than one of said strain, dry product, extract, culture and lysate, and a carrier. 15. Pharmaceutical composition for the prevention or treatment of a disease caused by glutathione deficiency, containing one or more than one material selected from the strain according to claim 1, the dry product of the specified strain, the extract of the specified strain, the culture of the specified strain and the lysate of the specified strain and glutathione isolated from any one or more of said strain, dry product, extract, culture and lysate, and a carrier.

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