RU2799433C2 - Regulatory elements of the plants and their use - Google Patents

Abstract

FIELD: biochemistry.

SUBSTANCE: invention relates to a recombinant DNA molecule for initiating transcription of a heterologous transcribed DNA molecule as well as to a genetic construct for initiating transcription of a heterologous transcribed DNA molecule containing it. Also the following are disclosed: a cell of a transgenic plant, a transgenic plant, a part of a transgenic plant, a transgenic seed containing the abovementioned DNA molecule. The invention also relates to a method of expressing a heterologous transcribed DNA molecule, as well as a method of obtaining a transgenic plant using the abovementioned DNA molecule.

EFFECT: invention makes it possible to effectively initiate transcription of a heterologous transcribed DNA molecule in a plant.

13 cl, 9 dwg, 20 tbl, 7 ex

Description

LINK TO RELATED APPLICATIONS

[0001] This application claims priority over US Provisional Application No. 61/739720, filed Dec. 19, 2012, which is incorporated herein in its entirety by reference.

INCLUDING A SEQUENCE LIST

[0002] The sequence listing, which is contained in a file called "MONS323WOseq.txt", 345 KB in size (measured on Microsoft Windows®), and was created on December 17, 2013, is submitted in electronic form and is incorporated herein by reference .

FIELD OF TECHNOLOGY

[0003] The present invention relates to the field of plant molecular biology, plant genetic engineering, and DNA molecules useful for modulating gene expression in plants.

BACKGROUND OF THE INVENTION

[0004] Regulatory elements are genetic elements that regulate the activity of genes by modulating the transcription of an operably linked transcribed DNA molecule. Such elements include promoters, leaders, enhancers, introns, and 3' untranslated regions and are useful in the field of plant molecular biology and plant genetic engineering.

SUMMARY OF THE INVENTION

[0005] The invention provides novel regulatory elements for use in plants and genetic constructs containing the regulatory elements. The invention also provides transgenic plant cells, plants, plant parts and seeds containing regulatory elements. In one embodiment, the invention provides the regulatory elements described herein operably linked to a transcribed DNA molecule. In some embodiments, the transcribed DNA molecule is heterologous with respect to the sequence of the regulatory element provided herein. This document also provides methods for the production and use of the regulatory elements described herein, including genetic constructs containing regulatory elements, and transgenic plants, plant cells, plant parts, and seeds containing regulatory elements operably linked to a transcribed DNA molecule that is heterologous in in relation to the regulatory element.

[0006] Thus, in one aspect, the invention provides a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any of SEQ ID NOs: 1- 98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. By "heterologous transcribed DNA molecule" is meant that the transcribed DNA molecule is heterologous with respect to the DNA sequence. In specific embodiments, the recombinant DNA molecule contains a DNA sequence having at least 90 percent, at least 91 percent, at least 92 percent, at least 93 percent, at least 94 percent, at least about 95 percent, at least 96 percent, at least 97 percent, at least 98 percent, or at least 99 percent sequence identity to the DNA sequence of any of SEQ ID NOs: 1-98 and 168-171. In specific embodiments, the heterologous transcribed DNA molecule contains a gene of agronomic interest, such as a gene capable of conferring resistance to herbicides or pests in plants. In yet other embodiments, the invention provides a genetic construct comprising a recombinant DNA molecule as provided herein.

[0007] In another aspect, provided herein are transgenic plant cells comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any of SEQ ID Nos. : 1-98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. In some embodiments, the transgenic plant cell is a monocot plant cell. In other embodiments, the transgenic plant cell is a dicot cell.

[0008] In yet another aspect, provided herein is a transgenic plant, or portion thereof, comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any from SEQ ID Nos. 1-98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. In specific embodiments of the invention, the transgenic plant is a daughter plant of any generation relative to the original transgenic plant and contains a recombinant DNA molecule. A transgenic seed containing a recombinant DNA molecule that produces such a transgenic plant when grown is also provided by the invention.

[0009] In yet another aspect, the invention provides a method for expressing a transcribed DNA molecule, e.g., a gene of agronomic interest, in a transgenic plant by obtaining a transgenic plant containing a recombinant DNA molecule of the invention and cultivating the plant.

[00010] This document also provides a method for providing a transgenic plant by transforming a plant cell with a recombinant DNA molecule of the invention to obtain a transformed plant cell and regenerating the transformed plant cell to obtain a transgenic plant.

BRIEF DESCRIPTION OF THE FIGURES

[00011] FIG. 1. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Agrostis nebulosa . In particular, Fig. 1 illustrates the alignment of the 2005 bp P-AGRne.Ubq1-1:1:5 promoter (SEQ ID NO: 2) included in the expression regulatory element (EXP) group EXP-AGRne.Ubq1:1 :7 (SEQ ID no: 1), with promoter variants P-AGRne.Ubq1-1:1:5. For example, a deletion at the 5' end of P-AGRne.Ubq1-1:1:5 produced the P-AGRne.Ubq1-1:1:4 promoter (SEQ ID NO: 6), a 999 bp sequence that is included in EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5). Another version of the promoter illustrated in FIG. 1 is P-AGRne.Ubq1-1:1:6 (SEQ ID NO: 8), a 762 bp sequence included in EXP-AGRne.Ubq1:1:9 (SEQ ID NO: 7).

[00012] FIG. 2. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Arundo donax . In particular, Fig. 2 illustrates a 4114 bp promoter alignment. P-ARUdo.Ubq1-1:1:4 (SEQ ID no: 10), included in the group of regulatory expression elements EXP-ARUdo.Ubq1:1:4 (SEQ ID no: 9), with P-ARUdo.Ubq1 promoter variants -1:1:4. The alignment includes: 2012 bp long promoter. P-ARUdo.Ubq1-1:1:5 (SEQ ID NO: 14); 1000 bp promoter P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); and a 755 bp promoter. P-ARUdo.Ubq1-1:1:8 (SEQ ID no: 22).

[00013] FIG. 3. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Arundo donax . In particular, Fig. 3 illustrates a 2033 bp promoter alignment. P-ARUdo.Ubq2-1:1:4 (SEQ ID NO: 24) with P-ARUdo.Ubq2-1:1:4 promoter variants. Included in the alignment are: a 2004 bp promoter. P-ARUdo.Ubq2-1:1:6 (SEQ ID NO: 28); 1001 bp promoter P-ARUdo.Ubq2-1:1:5 (SEQ ID no: 31); and a 696 bp promoter. P-ARUdo.Ubq2-1:1:7 (SEQ ID no: 33).

[00014] FIG. 4. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Bouteloua gracilis . In particular, Fig. 4 illustrates a 2371 bp promoter alignment. P-BOUgr.Ubq1-1:1:2 (SEQ ID NO: 35) with promoter variants at the 5'end of P-BOUgr.Ubq1-1:1:2. Included in the alignment are: 1999 bp promoter. P-BOUgr.Ubq1-1:1:3 (SEQ ID no: 39); 1022 bp promoter P-BOUgr.Ubq1-1:1:5 (SEQ ID no: 42); and a 760 bp promoter. P-BOUgr.Ubq1-1:1:6 (SEQ ID no: 44).

[00015] FIG. 5. Illustrates the alignment of several promoter variants of various sizes corresponding to promoter elements from Bouteloua gracilis . In particular, Fig. 5 illustrates the alignment of the 2100 bp promoter element. P-BOUgr.Ubq2-1:1:4 (SEQ ID NO: 46) with P-BOUgr.Ubq2-1:1:4 promoter variants. Included in the alignment: 2043 bp promoter. P-BOUgr.Ubq2-1:1:7 (SEQ ID no: 50); 2002 bp promoter P-BOUgr.Ubq2-1:1:5 (SEQ ID no: 53); 1024 bp promoter P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); and a 749 bp promoter. P-BOUgr.Ubq2-1:1:8 (SEQ ID no: 61).

[00016] FIG. 6. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Miscanthus sinensis . In particular, Fig. 6 illustrates the alignment of the 5359 bp promoter element. P-MISsi.Ubq1-1:1:2 (SEQ ID NO: 63) with P-MISsi.Ubq1-1:1:2 promoter variants. Included in the alignment are: 2423 bp promoter. P-MISsi.Ubq1-1:1:11 (SEQ ID no: 67); 1447 bp promoter P-MISsi.Ubq1-1:1:10 (SEQ ID no: 71); 899 bp promoter P-MISsi.Ubq1-1:1:13 (SEQ ID no: 73); 691 bp promoter P-MISsi.Ubq1-1:1:14 (SEQ ID no: 75); and a 506 bp promoter. P-MISsi.Ubq1-1:1:9 (SEQ ID no: 77).

[00017] FIG. 7. Illustrates the alignment of several promoter variants of various sizes corresponding to promoter elements from Schizachyium scoparium. In particular, Fig. 7 illustrates the alignment of the 2831 bp promoter element. P-SCHsc.Ubq1-1:1:12 (SEQ ID NO: 79) with P-SCHsc.Ubq1-1:1:12 promoter variants. Included in the alignment are: 2033 bp promoter. P-SCHsc.Ubq1-1:1:11 (SEQ ID no: 83); 1046 bp promoter P-SCHsc.Ubq1-1:1:10 (SEQ ID no: 85); and a 547 bp promoter. P-SCHsc.Ubq1-1:1:14 (SEQ ID no: 87).

[00018] FIG. 8. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Sorghastrum nutans . In particular, Fig. 8 illustrates the alignment of the 2218 bp promoter element. P-SORnu.Ubq1-1:1:4 (SEQ ID NO: 89) with P-SORnu.Ubq1-1:1:4 promoter variants. Included in the alignment are: 1964 bp promoter. P-SORnu.Ubq1-1:1:5 (SEQ ID no: 93); 1023 bp promoter P-SORnu.Ubq1-1:1:6 (SEQ ID no: 96); and a 724 bp promoter. P-SORnu.Ubq1-1:1:7 (SEQ ID no: 98).

[00019] FIG. 9. Illustrates the configurations of the expression cassettes of the invention.

BRIEF DESCRIPTION OF SEQUENCES

[00020] SEQ ID nos: 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41, 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108, 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150 and 168 are DNA sequences from regulatory expression element (EXP) groups containing a promoter sequence operably linked to a 5' leader sequence that is operably linked with the 5' intron sequence.

[00021] SEQ ID nos: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 and 169 are promoter sequences.

[00022] SEQ ID nos: 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152, and 170 are leader sequences.

[00023] SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 and 171 are intron sequences.

DETAILED DESCRIPTION OF THE INVENTION

[00024] The invention relates to DNA molecules having gene regulatory activity in plants. The nucleotide sequences of these DNA molecules are given in SEQ ID nos: 1-98 and 168-171. These DNA molecules are, for example, capable of influencing the expression of an operably linked transcribed DNA molecule in plant tissues and therefore regulating the expression of an operably linked transgene in transgenic plants. The invention also relates to methods for modifying, creating and using them. The invention also relates to compositions that include transgenic plant cells, plants, plant parts and seeds containing the recombinant DNA molecules of the invention, and to methods for their preparation and use.

[00025] The following definitions and methods are provided to better define the invention and to guide those skilled in the art in the practice of the invention. Unless otherwise indicated, the terms are to be understood in accordance with common usage by those skilled in the art.

DNA molecules

[00026] As used herein, the term "DNA" or "DNA molecule" refers to a double-stranded DNA molecule of cellular or synthetic origin, i.e., a polymer of deoxyribonucleotide bases. As used herein, the term "DNA sequence" refers to the nucleotide sequence of a DNA molecule. The nomenclature used in this document corresponds to that of 37 CFR 1.822 of the United States and is set forth in WIPO Standard ST.25 (1998) Schedules Appendix 2, Tables 1 and 3.

[00027] As used herein, a "recombinant DNA molecule" is a DNA molecule containing a combination of DNA molecules that would not naturally combine without human intervention. For example, a recombinant DNA molecule may be a DNA molecule that consists of at least two heterologous DNA molecules with respect to each other; a DNA molecule containing a DNA sequence that deviates from naturally occurring DNA sequences; or a DNA molecule that has been inserted into the host cell's DNA by genetic transformation.

[00028] As used herein, the term "sequence identity" refers to the degree to which two optimally aligned DNA sequences are identical. Optimal sequence alignment is created by manually aligning two DNA sequences, such as a reference sequence and another DNA sequence, to create the maximum number of matches between nucleotides while aligning sequences with corresponding internal nucleotide insertions, deletions, or breaks. As used herein, the term "reference sequence" refers to the DNA sequence given in SEQ ID nos: 1-98 and 168-171.

[00029] As used herein, the term "percent sequence identity" or "percent identity" or "% identity" is the proportion of identity multiplied by 100. The "proportion of identity" for a DNA sequence optimally aligned with the starting sequence is the number of matches between nucleotides in the optimal alignment divided by the total number of nucleotides in the reference sequence, for example , by the total number of nucleotides in the entire full-length reference sequence. Thus, in one embodiment, the invention provides a DNA molecule comprising a DNA sequence that, when optimally aligned with the reference sequence provided herein as SEQ ID NOs: 1-98 and 168-171, has at least about 85 percent identity. , at least about 86 percent identical, at least about 87 percent identical, at least about 88 percent identical, at least about 89 percent identical, at least about 90 percent identical, at least about 91 percent identical, at least about 92 percent identical, at least about 93 percent identical, at least about 94 percent identical, at least about 95 percent identical, at least about 96 percent identical, at least about 97 percent identical, at least about 98 percent identity, at least about 99 percent identity, or at least about 100 percent identity with the reference sequence.

Regulatory elements

[00030] Regulatory elements such as promoters, leaders, enhancers, introns, and transcription termination regions (or 3´ UTRs) play an essential role in overall gene expression in living cells. The term "regulatory element" as used herein refers to DNA molecules having a regulatory gene activity. The term "regulatory gene activity" as used herein refers to the ability to influence the expression of an operably linked transcribed DNA molecule, for example by affecting the transcription and/or translation of an operably linked transcribed DNA molecule. Regulatory elements such as promoters, leaders, enhancers and introns that function in plants are thus useful for modifying plant phenotypes through genetic engineering.

[00031] As used herein, an "expression regulatory element group" or "EXP" sequence may refer to a group of operably linked regulatory elements such as enhancers, promoters, leaders, and introns. Thus, a group of expression control elements may consist, for example, of a promoter operably linked to a 5' leader sequence, which in turn is operably linked to a 5' intron sequence.

[00032] Regulatory elements can be characterized by their gene expression pattern, e.g. by positive and/or negative influences such as constitutive expression or temporal, spatial, developmental, tissue, environmental, physiological, pathological, cell cycle and / or chemically sensitive expression, and any of their combinations, as well as quantitative or qualitative indicators. As used herein, the term "gene expression pattern" is any feature of the transcription of an operably linked DNA molecule into a transcribed RNA molecule. The transcribed RNA molecule may be translated to produce a protein molecule or may form an antisense or other regulatory RNA molecule such as double-stranded RNA (dsRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), miRNA (miRNA), and the like.

[00033] As used herein, the term "protein expression" is any feature of the translation of a transcribed RNA molecule into a protein molecule. The expression of a protein can be characterized by its temporal, spatial, developmental or morphological properties, as well as quantitatively or qualitatively.

[00034] A promoter is useful as a regulatory element for modulating the expression of an operably linked transcribed DNA molecule. As used herein, the term "promoter" refers generally to a DNA molecule that is involved in the recognition and binding of RNA polymerase II and other proteins, such as trans-acting transcription factors, to initiate transcription. The promoter may be derived from the 5'-untranslated region (5'-UTR) of the gene. Alternatively, promoters can be obtained synthetically or by manipulating the DNA molecule. Promoters can also be chimeric. Chimeric promoters are produced by the fusion of two or more heterologous DNA molecules. Promoters useful in the practice of the invention include SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, including fragments or variants thereof. In particular embodiments, such DNA molecules, and any variants or derivatives thereof described herein, are further defined as having promoter activity, i.e., capable of acting as a promoter in a host cell, such as a transgenic plant. In still further specific embodiments of the invention, the fragment may be defined as exhibiting the promoter activity that the original promoter molecule from which it was derived has, or the fragment may contain a "minimal promoter" that provides a basic level of transcription and includes a TATA box or an equivalent DNA sequence for recognition and binding of the RNA polymerase II complex to initiate transcription.

[00035] In one embodiment, the fragments are provided with the promoter sequence described herein. Promoter fragments may include promoter activity as described above and may be useful singly or in combination with other promoters and promoter fragments, for example in the design of chimeric promoters. Specific embodiments of the invention provide promoter fragments comprising at least about 50, at least about 75, at least about 95, at least about 100, at least about 125, at least about 150, at least about 175, at least about 200, at least about 225, at least about 250, at least about 275, at least about 300, at least about 500, at least about 600, at least about 700 , at least about 750, at least about 800, at least about 900, or at least about 1000 consecutive nucleotides, or more, of a DNA molecule having the promoter activity described herein. Methods for obtaining such fragments from the original promoter molecule are well known in the art.

[00036] Compositions derived from any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50 , 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, such as internal or 5' deletions, for example, can be obtained using methods well known in the art to improve or alter expression, including the removal of elements that have positive or negative effects on expression; doubling elements that have positive or negative effects on expression; and/or duplication or deletion of elements having tissue or cell specific effects on expression. Compositions derived from any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169 containing 3'-deletions in which the TATA-box element or its equivalent sequence The DNA and the underlying sequence removed can be used, for example, to create enhancer elements. Additional deletions can be made to remove any elements that have positive or negative, tissue-specific, cell-specific, or time-specific (such as, but not limited to, circadian rhythms) effects on expression. Any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61 , 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, and fragments or enhancers derived from them can be used to prepare compositions of chimeric regulatory elements, containing any of the promoters shown in SEQ ID nos: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, and fragments or enhancers derived therefrom operably linked to other enhancers and promoters.

[00037] In accordance with the present invention, a promoter or promoter fragment can be analyzed for known promoter elements, ie, DNA sequence characteristics such as the TATA box and other known transcription factor binding site motifs. The identification of such known promoter elements can be used by one of skill in the art to design promoter variants having a similar expression pattern as the parent promoter.

[00038] As used herein, the term "leader" refers to a DNA molecule from the 5'-untranslated region (5'-UTR) of a gene and is defined in a general sense as the DNA segment between the transcription initiation site (CIT) and the starting site of the protein coding sequence . Alternatively, the leaders can be obtained synthetically or by manipulating DNA elements. The leader can be used as a 5' regulatory element to modulate the expression of an operably linked transcribed DNA molecule. The leader molecules can be used with a heterologous promoter or with their native promoter. The promoter molecules of the invention may thus be operably linked to their native leader or may be operably linked to a heterologous leader. Leaders useful in the practice of the invention include SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170, or fragments or variants thereof. In specific embodiments of the invention, such DNA sequences can be defined as capable of acting as a leader in a host cell, including, for example, a transgenic plant cell. In one embodiment of the invention, such DNA sequences can be decoded as including leader activity.

[00039] The leader sequences (5'-UTRs) shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170 may be composed of regulatory elements or may take on secondary structures, affecting the transcription or translation of an operably linked DNA molecule. The leader sequences shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90 and 170 can be used according to the invention to create chimeric regulatory elements that affect transcription or translation of an operably linked DNA molecule. In addition, the leader sequences shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170 can be used to create chimeric leader sequences that affect transcription or translation of an operably linked DNA molecule. .

[00040] As used herein, the term "intron" refers to a DNA molecule that can be isolated or identified from a genomic copy of a gene and can be defined in a general sense as a region that is excised during messenger RNA (mRNA) processing prior to translation. Alternatively, the intron can be obtained synthetically or by manipulating a DNA element. The intron may contain enhancer elements that affect the transcription of functionally related genes. An intron can be used as a regulatory element to modulate the expression of an operably linked transcribed DNA molecule. The genetic construct may contain an intron, and the intron may or may not be heterologous to the DNA molecule being transcribed. Examples of introns in the art include the rice actin intron and the maize HSP70 intron.

[00041] Compared to genetic constructs without an intron, the inclusion of certain introns in genetic constructs results in increased accumulation of mRNA and protein in plants. This effect has been termed "intron mediated enhancement" (IME) of gene expression. Introns that stimulate expression in plants have been identified in maize genes ( e.g. tubA1, Adh1, Sh1, and Ubi1), in rice genes ( e.g. tpi ), and in genes of dicotyledonous plants such as petunia ( e.g. rbcS ), potato ( st -ls1 ) and Arabidopsis thaliana ( ubq3 and pat1 ) . Deletions or mutations within intron splicing sites have been shown to reduce gene expression, indicating that splicing may be required for IME. However, splicing per se is not required, as IME has been shown in dicotyledonous plants by point mutations within the splicing sites of the pat1 gene from A. thaliana . It has been shown that multiple use of the same intron in the same plant exhibits disadvantages. In such cases, it is necessary to have a collection of basic controls to generate the appropriate recombinant DNA elements.

[00042] Introns useful in the practice of the invention include SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91 , 94 and 171. Compositions derived from any of the introns shown in SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81 , 91, 94 and 171 may consist of internal deletions or duplications of cis-regulatory elements; and/or alterations to the 5' and 3' DNA sequences containing intron/exon splice junctions can be used to improve expression or expression specificity when operably linked to a promoter+leader or chimeric promoter+leader and a coding sequence. When changing intron/exon boundary sequences, it may be beneficial to avoid using the AT nucleotide sequence or A nucleotide sequence immediately before the 5' end of the splice site (GT) and the G nucleotide or TG nucleotide sequence, respectively, immediately after the 3' end of the splicing site (AG) , to eliminate potential unwanted start codons generated during the processing of the messenger RNA into the final transcript. The DNA sequence near the 5' or 3' end of the intron splicing sites can be modified in this way. Introns and intron variants altered as described herein and using methods known in the art can be tested empirically, as described in the Working Examples, to determine the effect of an intron on the expression of an operably linked DNA molecule.

[00043] As used herein, the term "3' transcription termination molecule", "3' untranslated region" or "3' UTR" refers to a DNA molecule that is used during transcription from the untranslated region of the 3' portion of an mRNA molecule. . The 3' untranslated region of an mRNA molecule can be obtained by specific cleavage and 3' polyadenylation, also known as a poly(A) tail. The 3'-UTR may be operably linked to and downstream of the transcribed DNA molecule, and may include a polyadenylation signal and other regulatory signals capable of affecting transcription, mRNA processing, or gene expression. It is believed that poly(A) tails perform the functions of mRNA stabilization and translation initiation. Examples of 3' transcription termination molecules in the art are the nopaline synthase 3' region, the wheat hsp17 3' region, the pea RuBisCO small subunit 3' region, the cotton 3' E6 region, and the 3' UTR of the coli.

[00044] 3'-UTRs generally find useful use for recombinant expression of specific DNA molecules. A weak 3'-UTR can generate a read-through that can affect the expression of a DNA molecule located in adjacent expression cassettes. A suitable control for transcription termination can prevent read-through into downstream DNA sequences ( eg other expression cassettes) and can further enable efficient reuse of RNA polymerase to improve gene expression. Efficient transcription termination (release of RNA polymerase II from DNA) is a prerequisite for the next transcription initiation and thus directly affects the overall transcript level. After transcription termination, the mature mRNA is released from the site of synthesis, and the template is transported into the cytoplasm. Eukaryotic mRNAs accumulate in poly(A) forms in vivo , making it difficult to detect transcription termination sites by conventional methods. However, prediction of functional and effective 3'-UTRs using bioinformatics methods is difficult, as there are no conserved DNA sequences that allow easy prediction of effective 3'-UTRs.

[00045] From a practical point of view, it is generally useful that the 3'-UTR used in the expression cassette has the following characteristics. The 3'-UTR must be able to rationally and efficiently complete transcription of the transgene and prevent read-through of the transcript in any of the adjacent DNA sequences that may be contained in another expression cassette, as in the case of several expression cassettes belonging to the same transfer DNA (T-DNA) , or adjacent chromosomal DNA into which the T-DNA has been inserted. The 3'-UTR should not lead to a decrease in the transcriptional activity conferred by the promoter, leader, enhancers and introns that are used to control the expression of the DNA molecule. In plant biotechnology, the 3'-UTR is often used to prime amplification reactions of reverse transcribed RNA isolated from transformed plants and is used to: (1) assess the transcriptional activity or expression of an expression cassette integrated into the plant chromosome; (2) estimates of the number of copies of inserts in plant DNA; and (3) assessing the zygosity of the resulting seed after propagation. The 3'-UTR is also used in amplification reactions of DNA extracted from transformed plants to determine the integrity of the inserted cassette.

[00046] As used herein, the term "enhancer" or "enhancer element" refers to a cis-acting regulatory element, referred to as a cis element, that confers an aspect of general character to expression, but generally not sufficient on its own to control transcription, functionally linked DNA sequence. Unlike promoters, enhancer elements typically do not include a transcription initiation site (TIT) or a TATA box or equivalent DNA sequence. A promoter or promoter fragment may naturally contain one or more enhancer elements that affect the transcription of an operably linked DNA sequence. An enhancer element can also be combined with a promoter to provide a chimeric cis promoter element conferring an aspect of general modulation of gene expression.

[00047] Many promoter enhancer elements are believed to bind DNA binding proteins and/or influence DNA topology by producing local conformations that selectively allow or restrict access of RNA polymerase to the DNA template, or that facilitate selective opening of the double helix at the initiation site. transcription. An enhancer element may also function to bind transcription factors that regulate transcription. Some enhancer elements bind more than one transcription factor, and transcription factors can interact with different affinities with more than one enhancer domain. Enhancer elements can be identified by a number of methods, including analysis of deletions, ie removal of one or more nucleotides from the 5' end or within the promoter; DNA binding protein analysis using DNase I footprinting, methylation interference, electrophoretic mobility shift assays, in vivo genomic footprinting by ligation-mediated polymerase chain reaction (PCR) and other conventional assays; or by DNA sequence similarity analysis using known cis element or enhancer element motifs as target sequence or target motif with conventional DNA sequence comparison methods such as BLAST. The fine structure of the enhancer domain can be further explored by mutagenesis (or substitution) of one or more nucleotides, or by other conventional methods known in the art. Enhancer elements can be obtained by chemical synthesis or by isolation from regulatory elements that include such elements, and they can be synthesized with additional flanking nucleotides containing applicable restriction sites to facilitate subsequent manipulations. Thus, the design, construction and use of enhancer elements, in accordance with the methods described herein, to modulate the expression of operably linked transcribed DNA molecules are within the scope of the invention.

[00048] As used herein, the term "chimeric" refers to a single DNA molecule obtained by fusing a first DNA molecule with a second DNA molecule, where neither the first nor the second DNA molecule would normally be contained in such a configuration, i.e., not will be merged with each other. A chimeric DNA molecule is thus a new DNA molecule, in other words, not normally found in nature. As used herein, the term "chimeric promoter" refers to a promoter obtained by such manipulation of DNA molecules. A chimeric promoter may combine two or more DNA fragments, such as fusion of a promoter with an enhancer element. Thus, the design, construction and use of chimeric promoters, in accordance with the methods described herein, to modulate the expression of operably linked transcribed DNA molecules are within the scope of the invention.

[00049] As used herein, the term "variant" refers to a second DNA molecule, such as a regulatory element, that is similar in composition to, but not identical to, the first DNA molecule, and wherein the second DNA molecule still retains overall functionality, i.e. such the same or similar expression pattern, for example, through greater or lesser or equivalent transcriptional or translational activity compared to the first DNA molecule. The variant may be a shortened or truncated version of the first DNA molecule and/or an altered version of the DNA sequence of the first DNA molecule, such as a variant with different restriction sites and/or internal deletions, substitutions and/or insertions. "Variants" of a regulatory element also encompasses variants resulting from mutations that occur during or as a result of transformation of bacterial and plant cells. In the invention, the DNA sequence given in SEQ ID NOs: 1-98 and 168-171 can be used to generate variants that are similar in composition, but not identical to the DNA sequence of the original regulatory element, while still retaining common functionality, i.e. That is, the same or similar pattern of expression as the original regulatory element. The preparation of such embodiments of the invention is within the ordinary skill of the art in light of the description and is within the scope of the invention.

[00050] Chimeric regulatory elements can be designed to include various constituent elements that can be operably linked in various ways known in the art, such as restriction enzyme digestion and ligation, ligation-independent cloning, modular assembly of PCR products during amplification, or direct chemical synthesis of the regulatory element, as well as by other methods known in the art. The resulting different chimeric regulatory elements may be composed of the same or variants of the same building blocks, but differ in DNA sequence, or DNA sequences that include a DNA binding sequence, or sequences that allow the building blocks to be operably linked. In the invention, the DNA sequence set forth in SEQ ID NOs: 1-98 and 168-171 may provide a regulatory element reference sequence in which constituent elements comprising the reference sequence may be joined by methods known in the art and may include substitutions, deletions and/or insertions of one or more nucleotides or mutations that are commonly encountered during the transformation of bacterial and plant cells.

[00051] The effectiveness of the modifications, duplications or deletions described herein on the desired aspects of the expression of a particular transgene can be tested empirically in stable and transient plant tests, such as those described in the working examples herein, in order to confirm the results. , which may vary depending on the changes made and the purpose of the change in the original DNA molecule.

genetic constructs

[00052] As used herein, the term "genetic construct" means any recombinant DNA molecule, such as a plasmid, cosmid, virus, phage, or linear or circular DNA or RNA molecule, obtained from any source, capable of genomic integration or autonomous replication, including a DNA molecule, where at least one DNA molecule is linked to another DNA molecule in a functionally effective way, i.e. functionally linked. Used in this document, the term "vector" means any genetic construct that can be used for the purposes of transformation, ie, the introduction of heterologous DNA or RNA into a host cell. The genetic construct typically includes one or more expression cassettes. As used herein, "expression cassette" refers to a DNA molecule containing at least a transcribed DNA molecule operably linked to one or more regulatory elements, typically at least a promoter and a 3'-UTR.

[00053] As used herein, the term "operably linked" refers to a first DNA molecule connected to a second DNA molecule, wherein the first and second DNA molecules are arranged such that the first DNA molecule affects the function of the second DNA molecule. The two DNA molecules may or may not be part of a single continuous DNA molecule and may or may not be adjacent. For example, a promoter is operably linked to a transcribed DNA molecule if the promoter modulates the transcription of the target transcribed DNA molecule in the cell. A leader, for example, is operably linked to a DNA sequence when it is capable of affecting transcription or translation of the DNA sequence.

[00054] The genetic constructs of the invention can be provided in one embodiment as genetic constructs consisting of two tumor-inducing plasmid border regions, (Ti), having right border region (RB or AGRtu.RB) and left border region (LB or AGRtu.LB) Ti-plasmid isolated from Agrobacterium tumefaciens containing T-DNA, which, along with the molecular transfer provided by A. tumefaciens cells, allows the integration of T-DNA into the plant cell genome ( see, for example , US Pat. No. 6,603,061 ). Genetic constructs may also contain segments of a plasmid DNA backbone that provide replication function and selection for an antibiotic in bacterial cells, such as an Escherichia coli origin of replication such as ori322, a broad host-range origin of replication such as oriV or oriRi, and a selectable marker coding region. , such as Spec/Strp encoding a Tn7 aminoglycoside adenyltransferase ( aadA ) conferring resistance to spectinomycin or streptomycin, or a gentamicin selectable marker gene (Gm, Gent). A. tumefaciens bacterial host strain ABI, C58 or LBA4404 is often used for plant transformation; however, other strains known to those skilled in the art of plant transformation may function in the invention.

[00055] Methods are known in the art for assembling and introducing genetic constructs into a cell such that a transcribed DNA molecule is transcribed into a functional mRNA molecule that is translated and expressed as a protein. For the practice of the invention, conventional compositions and methods for the preparation and use of genetic constructs and host cells are well known to those skilled in the art. Typical vectors used for nucleic acid expression in higher plants are well known in the art and include vectors derived from Agrobacterium tumefaciens Ti plasmid and pCaMVCN transfer control vector.

[00056] Various regulatory elements may be included in the genetic construct, including any of those proposed herein. Any such regulatory elements may be provided in combination with other regulatory elements. Such combinations can be designed or modified to obtain the desired regulatory functions. In one embodiment, the genetic constructs of the invention comprise at least one regulatory element operably linked to a transcribed DNA molecule operably linked to a 3'UTR.

[00057] The genetic constructs of the invention may include any promoter or leader as provided herein or known in the art. For example, a promoter of the invention may be operably linked to a heterologous untranslated 5' leader, such as one derived from a heat shock protein gene. Alternatively, the leader of the invention may be operably linked to a heterologous promoter such as the cauliflower mosaic virus 35S promoter transcript.

[00058] Expression cassettes may also include a transit peptide coding sequence that encodes a peptide useful for intracellular targeting of an operably linked protein, particularly to a chloroplast, leukoplast, or other plastid organelle, mitochondria, peroxisome, vacuole, or extracellular space. Many proteins localized in chloroplasts are expressed as precursors by nuclear genes and are directed to the chloroplast via the chloroplast transit peptide (CTP). Examples of such proteins isolated from chloroplasts include, but are not limited to, ribulose-1,5-bisphosphate carboxylase small subunit (MCE)-associated proteins, ferredoxin, ferredoxin oxidoreductase, photosensitizing complex protein I and protein II, thioredoxin F, and enolpyruvil shikimate phosphate synthase (EPSPS). Chloroplast transit peptides are described, for example, in US Pat. No. 7,193,133. It has been shown that non-chloroplast proteins can be targeted to the chloroplast upon expression of a heterologous CTP operably linked to a transgene encoding non-chloroplast proteins.

transcribed DNA molecule

[00059] As used herein, the term "transcribable DNA molecule" refers to any DNA molecule capable of being transcribed into an RNA molecule, including, but not limited to, those that have protein-coding sequences and those that produce RNA molecules having sequences useful for gene suppression. The type of DNA molecule may include, but is not limited to, a DNA molecule from the same plant, a DNA molecule from another plant, a DNA molecule from another organism, or a synthetic DNA molecule such as a DNA molecule containing an antisense gene sequence or a DNA molecule encoding an artificial , a synthetic or otherwise modified version of the transgene. An exemplary transcribed DNA molecule for inclusion in the genetic constructs of the invention includes, for example , DNA molecules or genes from a different species than the one in which the DNA molecule is inserted, or genes derived from or present in the same species, but which are included in cells - recipients by means of genetic engineering, and not by classical methods of reproduction.

[00060] "Transgene" refers to a transcribed DNA molecule that is heterologous to a host cell, at least in terms of its location in the host cell's genome and/or a transcribed DNA molecule artificially incorporated into the host cell's genome in the current or any previous generation of cells.

[00061] A regulatory element, such as a promoter of the invention, may be operably linked to a transcribed DNA molecule that is heterologous to the regulatory element. As used herein, the term "heterologous" refers to a combination of two or more DNA molecules when such a combination does not normally occur in nature. For example, the two DNA molecules may be from different species and/or the two DNA molecules may be from different genes, such as different genes from the same species or the same genes from different species. A regulatory element is thus heterologous to an operably linked transcribed DNA molecule if such combination does not normally occur in nature, i.e. the transcribed DNA molecule does not normally occur operably linked to the regulatory element.

[00062] A transcribed DNA molecule can generally be any DNA molecule for which expression of a transcript is desired. Such transcript expression can lead to translation of the resulting mRNA molecule and, accordingly, to protein expression. Alternatively, for example, a transcribed DNA molecule can be designed to ultimately cause a decrease in the expression of a particular gene or protein. In one embodiment of the invention, this can be achieved with a transcribed DNA molecule oriented in the antisense direction. One of skill in the art is familiar with the use of such antisense technology. Any gene can be negatively regulated in this way, and in one embodiment of the invention, the transcribed DNA molecule can be designed to suppress a particular gene through the expression of a dsRNA, siRNA, or microRNA molecule.

[00063] Thus, in one embodiment, there is provided a recombinant DNA molecule comprising a regulatory element of the invention, such as those set forth in SEQ ID NOs: 1-98 and 168-171, operably linked to a heterologous transcribed DNA molecule for in order to modulate the transcription of the transcribed DNA molecule at the desired level or pattern when the genetic construct is integrated into the genome of the transgenic plant cell. In one embodiment of the invention, the transcribed DNA molecule contains the region of the gene encoding the protein, and in another embodiment of the invention, the transcribed DNA molecule contains the antisense region of the gene.

Genes of agronomic interest

[00064] The DNA molecule to be transcribed may be a gene of agronomic interest. As used herein, the term "gene of agronomic interest" refers to a transcribed DNA molecule that, when expressed in a particular plant tissue, cell, or cell type, confers a desired characteristic. A gene product of agronomic interest may act within a plant to influence plant morphology, physiology, growth, development, yield, grain composition, nutritional profile, disease or pest resistance, and/or environmental or chemical tolerance. , or may act as a pesticidal agent in the diet of a pest that feeds on the plant. In one embodiment, the regulatory element of the invention is incorporated into a genetic construct such that the regulatory element is operably linked to a transcribed DNA molecule that is a gene of agronomic interest. In a transgenic plant containing such a genetic construct, expression of a gene of agronomic interest can confer a beneficial agronomic trait. A beneficial agronomic trait may include, but is not limited to, for example, herbicide tolerance, insect control, yield modification, disease resistance, pathogen resistance, plant growth and development modification, modified starch content, modified oil content, modified fatty acid content , modified protein content, modified fruit ripening, improved animal and human nutrition, biopolymer production, environmental stress tolerance, pharmaceutical peptides, improved processing properties, improved taste, beneficial hybrid seed production, improved fiber production, and desirable biofuel production.

[00065] Examples of genes of agronomic interest known in the art include herbicide resistance (US Pat. Nos. 6,803,501, 6,448,476, 6,248,876, 6,225,114, yield (U.S. Patent No. Nos. USRE38446, 6716474, 6663906, 6476295, 6441277, 6423828, 6399330, 6372211, 6235971, 6222098 and 5716837), insect control (U.S. Patent Nos. 6809078, 6713063, 6686452, 6657046, 6645497, 6642030, 6639054, 6620988, 6593293, 6555655 6538109 6537756 6521442 6501009 6468523 6326351 6313378 6284949 6281016 6248536 6242241 6221649 6177 615 6156573 6153814 6110464 6093695 6063756 6063597 6023013 5959091 5942664 5942658 5880275 5,763,245 and 5,763,241), resistance to fungal diseases (US Pat. resistance to viruses (US patents No. 6617496, 6608241, 6015940, 6013864 , 5,850,023 and 5,304,730), nematode resistance (US Pat. No. 6,228,992), bacterial disease resistance (US Pat. No. 5,516,671), plant growth and development (US Pat. Nos. 6,723,897 and 6,518,488), starch accumulation (US Pat. 6538179, 6538178, 5750876, 6476295), production of modified oils (US patents Nos. 6444876, 6426447 and 6380462), increased production of oils (US patents Nos. fatty acid content (U.S. Patent Nos. 6828475, 6822141, 6770465, 6706950, 6660849, 6596538, 6589767, 6537750, 6489461 and 6459018), increased protein production (US patent No. 6380466), fruit ripening (US patent No. 55124 66), improved animal and human nutrition (U.S. Patent Nos. 6723837, 6653530, 6541259; 5,985,605 and 6,171,640), biopolymers (US Pat. 6140075 and 6,080,560), improved processing properties (U.S. Patent No. 6,476,295), improved digestibility (U.S. Patent No. 6,531,648), low raffinose (U.S. Patent No. 6,166,292), commercial production of enzyme (U.S. Patent No. 6,011,199), nitrogen fixation (U.S. Patent No. 5,229,114), hybrid seed production (U.S. Patent No. 5,689,041), fiber production (U.S. Patents Nos. 6,576,818, 6,271,443, 5,981,834 and 5,869,720), and biofuel production (U.S. Patent No. 5,998,700).

[00066] Alternatively, a gene of agronomic interest can affect the aforementioned plant characteristics or phenotypes by encoding an RNA molecule that causes targeted modulation of endogenous gene expression, such as through antisense ( see, for example , US Pat. No. 5,107,065), inhibitory RNA ("RNA interferences" involving the modulation of gene expression by microRNA, miRNA, trans-acting miRNA and phasic small RNA-mediated mechanisms, e.g. as described in US Published Applications 2006/0200878 and 2008/0066206 and in US Patent Application 11/974469); or cosuppressor-mediated mechanisms. The RNA can also be a catalytic RNA molecule ( eg ribozyme or riboswitch, see eg US Published Application 2006/0200878) designed to cleave the desired endogenous mRNA product. Methods are known in the art for designing and introducing genetic constructs into a cell such that a transcribed DNA molecule is transcribed into a molecule capable of inducing gene suppression.

[00067] Expression of a transcribed DNA molecule in a plant cell can also be used to suppress plant pests that feed on plant cells, such as compositions isolated from beetle pests and compositions isolated from nematode pests. Plant pests include, but are not limited to, arthropod pests, nematode pests, and fungal or microbial pests.

selective markers

[00068] Selectable marker transgenes can also be used with the regulatory elements of the invention. As used herein, the term "selectable marker transgene" refers to any transcribed DNA molecule whose expression in the transgenic plant, tissue or cell, or lack thereof, can be screened or counted by some method. Selectable marker genes and associated selection and screening methods for use in the practice of the invention are known in the art and include, but are not limited to, transcribed DNA molecules encoding beta-glucuronidase (GUS), green fluorescent protein ( GFP), proteins conferring antibiotic resistance and proteins conferring herbicide resistance.

Cell transformation

[00069] The invention is also directed to a method for producing transformed cells and plants containing one or more regulatory elements operably linked to the transcribed DNA molecule.

[00070] The term "transformation" refers to the introduction of a DNA molecule into a recipient host. As used herein, the term "host" refers to bacteria, fungi, or plants, including any cells, tissues, organs, or progeny of bacteria, fungi, or plants. Plant tissues and cells of particular interest include protoplasts, callus, roots, tubers, seeds, stems, leaves, seedlings, ovaries and pollen.

[00071] As used herein, the term "transformed" refers to a cell, tissue, organ, or organism into which a foreign DNA molecule, such as a genetic construct, has been introduced. The introduced DNA molecule can be integrated into the genomic DNA of a cell, tissue, organ or organism of the recipient such that the introduced DNA molecule is inherited in future offspring. A "transgenic" or "transformed" cell or organism may also include the progeny of the cell or organism and the progeny resulting from a breeding program using such a transgenic organism as the parent in the cross and exhibiting an altered phenotype as a result of the presence of foreign DNA molecules. The introduced DNA molecule may also be temporarily introduced into the recipient cell such that the introduced DNA molecule is not inherited in further offspring. The term "transgenic" refers to a bacterium, fungus or plant containing one or more heterologous DNA molecules.

[00072] There are many methods, well known to those skilled in the art, for introducing a DNA molecule into plant cells. The process typically includes the steps of selecting an appropriate host cell, transforming the host cell with a vector, and obtaining the transformed host cell. Methods and materials for transforming plant cells, by introducing a plant genetic construct into the plant genome, in practice of the invention may include any of the known and shown methods. Suitable methods include, but are not limited to, bacterial infection ( e.g. , Agrobacterium ), binary IIR vectors, direct DNA transfer ( e.g. , PEG-mediated transformation, drying/inhibition-mediated DNA uptake, electroporation, mixing with silicon carbide fibers, and particle acceleration covered with DNA) from among others.

[00073] The host cells can be any cell or organism, such as a plant cell, an algal cell, an algae, a fungal cell, a fungus, a bacterial cell, or an insect cell. In specific embodiments of the invention, host cells and transformed cells may include cultured plant cells.

[00074] The transgenic plant can subsequently be regenerated from the transgenic plant cell of the invention. Seeds can be obtained from this transgenic plant using conventional propagation or self-pollination techniques. Such seed and the resulting progeny plant grown from such seed will contain the recombinant DNA molecule of the invention and will therefore be transgenic.

[00075] The transgenic plants of the invention may be self-pollinated to provide seeds of homozygous transgenic plants of the invention (homozygous for the recombinant DNA molecule), or crossed with non-transgenic plants or other transgenic plants to provide seeds of the homozygous transgenic plants of the invention (homozygous for the recombinant DNA molecule DNA). Both homozygous and heterozygous transgenic plants are referred to herein as "progeny plants". Descendant plants are transgenic plants derived from the original transgenic plant and containing the recombinant DNA molecule of the invention. Seeds produced using a transgenic plant of the invention can be collected and used to grow generations of transgenic plants, ie progeny plants of the invention, comprising the genetic construct of the invention and expression of a gene of agronomic interest. Descriptions of propagation methods commonly used for various crops can be found in one of several book references, see for example Allard, Principles of Plant Breeding , John Wiley & Sons, NY, U. of CA, Davis, CA, 50 -98 (1960); Simmonds, Principles of Crop Improvement , Longman, Inc., NY, 369-399 (1979); Sneep and Hendriksen, Plant breeding Perspectives , Wageningen (ed), Center for Agricultural Publishing and Documentation (1979); Fehr, Soybeans: Improvement, Production and Uses , 2nd Edition, Monograph, 16:249 (1987); Fehr, Principles of Variety Development , Theory and Technique, (Vol. 1) and Crop Species Soybean (Vol. 2), Iowa State Univ., Macmillan Pub. Co., NY, 360-376 (1987).

[00076] Transformed plants can be analyzed for the presence of the target gene or genes and for the level of expression and/or profile provided by the regulatory elements of the invention. Those skilled in the art are aware of the numerous methods available for analyzing transformed plants. For example, plant analysis methods include, but are not limited to, Southern blots or Northern blots, PCR-based approaches, biochemical assays, phenotypic screening methods, field trials, and immunodiagnostic assays. Expression of the transcribed DNA molecule can be measured using TaqMan® reagents (Applied Biosystems, Foster City, CA) and by the methods described by the manufacturer and by the number of PCR cycles determined using the TaqMan® Testing Matrix. Alternatively, Invader® reagents (Third Wave Technologies, Madison, WI) and methods described by the manufacturer can be used to evaluate transgene expression.

[00077] The invention also relates to parts of the plant according to the invention. Plant parts include, but are not limited to, leaves, stems, roots, tubers, seeds, endosperm, ovule, and pollen. Parts of plants according to the invention may be viable, non-viable, capable and/or not capable of regeneration. The invention also includes and provides transformed plant cells containing the DNA molecule of the invention. Transformed cells or cells of a transgenic plant according to the invention include cells capable and/or not capable of regeneration.

[00078] The invention may be more readily understood by reference to the following examples, which are provided by way of illustration and are not intended to limit the invention unless stated otherwise. It will be appreciated by those skilled in the art that the methods described in the following examples are the methods found by the inventors to function normally in the practice of the invention. However, it should be clear to those skilled in the art, in light of the present disclosure, that many changes can be made to the specific embodiments of the invention described and still produce a similar or analogous result without departing from the spirit and scope of the invention, so anything of significance is placed hereinafter or shown in the accompanying graphics and should be interpreted in an illustrative and not limiting sense.

EXAMPLES

Example 1

Identification and cloning of regulatory elements

[00079] Novel ubiquitin regulatory elements, or Expression Regulatory Element (EXP) group sequences, have been identified and isolated from the genomic DNA of monocots: dark bentgrass ( Agrostis nebulosa ), cane arundo ( Arundo donax ), graceful buteloui ( Bouteloua gracilis ), Chinese miscanthus ( Miscanthus sinensis ), panicled schizachyrium ( Schizachyrium scoparium ), drooping sorghastrum ( Sorghastrum nutans ) and coli ( Coix lacryma-jobi ).

[00080] Ubiquitin 1 transcript sequences have been identified in each of the above species. The 5'-untranslated region (5'-UTR) of each of the ubiquitin 1 transcripts was used to design primers for the amplification of the corresponding regulatory elements of the identified ubiquitin gene, which includes a promoter, a leader (5'-UTR), and the first intron functionally linked to each other. The primers were used with GenomeWalker ^™ (Clontech Laboratories, Inc, Mountain View, CA) libraries designed according to the manufacturer's protocol to clone the 5' region of the corresponding genomic DNA sequence. Ubiquitin regulatory elements have also been isolated from the monocots Setaria italica , Setaria viridis , and Zea mays subsp. Mexicana (Teosinte) using the GenomeWalker ^™ libraries as described above. In addition, ubiquitin regulatory elements have also been isolated from the monocot Sorghum bicolor using publicly available sequences that are homologues to ubiquitin genes 4, 6, and 7.

[00081] Using the identified sequences, bioinformatic analysis was performed to identify regulatory elements within the amplified DNA. Using the results of this analysis, regulatory elements were identified within the DNA sequences and primers designed to amplify the regulatory elements. The corresponding DNA molecule for each regulatory element was amplified using standard polymerase chain reaction (PCR) conditions with primers containing unique restriction sites and genomic DNA isolated from Aβ . nebulosa, A donax, B. gracilis, M. sinensis, S. scoparium , S. nutans, and C. lacryma-jobi . The resulting DNA fragments were ligated into basic plant expression vectors and sequenced. Then, the transcription initiation site (TSIT) of the regulatory element and the intron/exon of the splicing junction were analyzed using transformed plant protoplasts. Briefly, protoplasts were transformed with plant expression vectors containing cloned DNA fragments operably linked to a heterologous transcribed DNA molecule, and the 5'-RACE system for rapid cDNA end amplification, version 2.0 (Invitrogen, Carlsbad, California 92008) was used to confirm SIT. regulatory element and intron/exon of the splicing junction by analyzing the sequence of messenger RNA (mRNA) transcripts obtained in this way.

[00082] The DNA sequences identified by EXP are provided herein as SEQ ID NOs: 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41 , 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108 , 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150, and 168, which are shown in Table 1 below. No.: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 and 169. Leader sequences are provided herein as SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152 and 170. Intron sequences provided herein as SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 and 171.

Table 1. Regulatory expression element ("EXP") groups, promoters, enhancers, leaders, and introns isolated from various cereal species.

Description SEQID No.: Size (p. n.) Genus/Species Description and/or regulatory elements from EXP, connected in 5' → 3' direction (SEQ ID no.): EXP-AGRne.Ubq1:1:7 1 3143 A. nebulosa EXP: P-AGRne.Ubq1-1:1:5 (SEQ ID no: 2); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:5 2 2005 A. nebulosa promoter L-AGRne.Ubq1-1:1:1 3 85 A. nebulosa Leader I-AGRne.Ubq1-1:1:3 4 1053 A. nebulosa Intron EXP-AGRne.Ubq1:1:8 5 2137 A. nebulosa EXP: P-AGRne.Ubq1-1:1:4 (SEQ ID no: 6); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:4 6 999 A. nebulosa promoter EXP-AGRne.Ubq1:1:9 7 1900 A. nebulosa EXP: P-AGRne.Ubq1-1:1:6 (SEQ ID no: 8); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:6 8 762 A. nebulosa promoter EXP-ARUdo.Ubq1:1:4 9 5068 A.donax EXP: P-ARUdo.Ubq1-1:1:4 (SEQ ID no: 10); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:2 (SEQ ID no: 12) P-ARUdo.Ubq1-1:1:4 10 4114 A.donax promoter L-ARUdo.Ubq1-1:1:1 eleven 85 A.donax Leader I-ARUdo.Ubq1-1:1:2 12 869 A.donax Intron EXP-ARUdo.Ubq1:1:8 13 2969 A.donax EXP: P-ARUdo.Ubq1-1:1:5 (SEQ ID no: 14); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) P-ARUdo.Ubq1-1:1:5 14 2012 A.donax promoter I-ARUdo.Ubq1-1:1:3 15 872 A.donax Intron EXP-ARUdo.Ubq1:1:6 16 1954 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:2 (SEQ ID no: 12) P-ARUdo.Ubq1-1:1:6 17 1000 A.donax promoter EXP-ARUdo.Ubq1:1:9 18 1957 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) EXP-ARUdo.Ubq1:1:12 19 1957 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:4 (SEQ ID no: 20) I-ARUdo.Ubq1-1:1:4 20 872 A.donax Intron EXP-ARUdo.Ubq1:1:11 21 1712 A.donax EXP: P-ARUdo.Ubq1-1:1:8 (SEQ ID no: 22); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) P-ARUdo.Ubq1-1:1:8 22 755 A.donax promoter EXP-ARUdo.Ubq2:1:4 23 3276 A.donax EXP: P-ARUdo.Ubq2-1:1:4 (SEQ ID no: 24); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:1 (SEQ ID no: 26) P-ARUdo.Ubq2-1:1:4 24 2033 A.donax promoter L-ARUdo.Ubq2-1:1:1 25 88 A.donax Leader I-ARUdo.Ubq2-1:1:1 26 1155 A.donax Intron EXP-ARUdo.Ubq2:1:8 27 3250 A.donax EXP: P-ARUdo.Ubq2-1:1:6 (SEQ ID no: 28); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:6 28 2004 A.donax promoter I-ARUdo.Ubq2-1:1:2 29 1158 A.donax Intron EXP-ARUdo.Ubq2:1:9 thirty 2247 A.donax EXP: P-ARUdo.Ubq2-1:1:5 (SEQ ID no: no. 31); L-ARUdo.Ubq2-1:1:1 (SEQ ID NO: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:5 31 1001 A.donax promoter EXP-ARUdo.Ubq2:1:10 32 1942 A.donax EXP: P-ARUdo.Ubq2-1:1:7 (SEQ ID no: 33); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:7 33 696 A.donax promoter EXP-BOUgr.Ubq1:1:1 34 3511 B. gracilis EXP: P-BOUgr.Ubq1-1:1:2 (SEQ ID no: 35); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:2 (SEQ ID no: 37) P-BOUgr.Ubq1-1:1:2 35 2371 B. gracilis promoter L-BOUgr.Ubq1-1:1:1 36 86 B. gracilis Leader I-BOUgr.Ubq1-1:1:2 37 1054 B. gracilis Intron EXP-BOUgr.Ubq1:1:6 38 3142 B. gracilis EXP: P-BOUgr.Ubq1-1:1:3 (SEQ ID no: 39); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:3 39 1999 B. gracilis promoter I-BOUgr.Ubq1-1:1:3 40 1057 B. gracilis Intron EXP-BOUgr.Ubq1:1:7 41 2165 B. gracilis EXP: P-BOUgr.Ubq1-1:1:5 (SEQ ID no: 42); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:5 42 1022 B. gracilis promoter EXP-BOUgr.Ubq1:1:8 43 1903 B. gracilis EXP: P-BOUgr.Ubq1-1:1:6 (SEQ ID no: 44); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:6 44 760 B. gracilis promoter EXP-BOUgr.Ubq2:1:11 45 3234 B. gracilis EXP: P-BOUgr.Ubq2-1:1:4 (SEQ ID no: 46); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:3 (SEQ ID no: 48) P-BOUgr.Ubq2-1:1:4 46 2100 B. gracilis promoter L-BOUgr.Ubq2-1:1:1 47 91 B. gracilis Leader I-BOUgr.Ubq2-1:1:3 48 1043 B. gracilis Intron EXP-BOUgr.Ubq2:1:7 49 3176 B. gracilis EXP: P-BOUgr.Ubq2-1:1:7 (SEQ ID no: 50); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:1 (SEQ ID no: 51) P-BOUgr.Ubq2-1:1:7 50 2043 B. gracilis promoter I-BOUgr.Ubq2-1:1:1 51 1042 B. gracilis Intron EXP-BOUgr.Ubq2:1:14 52 3139 B. gracilis EXP: P-BOUgr.Ubq2-1:1:5 (SEQ ID no: 53); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:4 (SEQ ID no: 54) P-BOUgr.Ubq2-1:1:5 53 2002 B. gracilis promoter I-BOUgr.Ubq2-1:1:4 54 1046 B. gracilis Intron EXP-BOUgr.Ubq2:1:15 55 2160 B. gracilis EXP: P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:5 (SEQ ID no: 57) P-BOUgr.Ubq2-1:1:6 56 1024 B. gracilis promoter I-BOUgr.Ubq2-1:1:5 57 1045 B. gracilis Intron EXP-BOUgr.Ubq2:1:16 58 2160 B. gracilis EXP: P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:6 (SEQ ID no: 59) I-BOUgr.Ubq2-1:1:6 59 1045 B. gracilis Intron EXP-BOUgr.Ubq2:1:17 60 1885 B. gracilis EXP: P-BOUgr.Ubq2-1:1:8 (SEQ ID no: 61); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:6 (SEQ ID no: 59) P-BOUgr.Ubq2-1:1:8 61 749 B. gracilis promoter EXP-MISsi.Ubq1:1:2 62 6813 M. sinensis EXP: P-MISsi.Ubq1-1:1:2 (SEQ ID no: 63); L-MISsi.Ubq1-1:1:1 (SEQ ID no: 64); I-MISsi.Ubq1-1:1:1 (SEQ ID no: 65) P-MISsi.Ubq1-1:1:2 63 5359 M. sinensis promoter L-MISsi.Ubq1-1:1:1 64 63 M. sinensis Leader I-MISsi.Ubq1-1:1:1 65 1391 M. sinensis Intron EXP-MISsi.Ubq1:1:9 66 4402 M. sinensis EXP: P-MISsi.Ubq1-1:1:11 (SEQ ID no: 67); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:11 67 2423 M. sinensis promoter L-MISsi.Ubq1-1:1:2 68 55 M. sinensis Leader I-MISsi.Ubq1-1:1:3 69 1924 M. sinensis Intron EXP-MISsi.Ubq1:1:8 70 3426 M. sinensis EXP: P-MISsi.Ubq1-1:1:10 (SEQ ID no: 71); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:10 71 1447 M. sinensis promoter EXP-MISsi.Ubq1:1:10 72 2878 M. sinensis EXP: P-MISsi.Ubq1-1:1:13 (SEQ ID no: 73); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:13 73 899 M. sinensis promoter EXP-MISsi.Ubq1:1:11 74 2670 M. sinensis EXP: P-MISsi.Ubq1-1:1:14 (SEQ ID no: 75); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:14 75 691 M. sinensis promoter EXP-MISsi.Ubq1:1:7 76 2485 M. sinensis EXP: P-MISsi.Ubq1-1:1:9 (SEQ ID no: 77); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:9 77 506 M. sinensis promoter EXP-SCHsc.Ubq1:1:9, 78 4079 S. scoparium EXP: P-SCHsc.Ubq1-1:1:12 (SEQ ID no: 79); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:12 79 2831 S. scoparium promoter L-SCHsc.Ubq1-1:1:3 80 95 S. scoparium Leader I-SCHsc.Ubq1-1:1:2 81 1153 S. scoparium Intron EXP-SCHsc.Ubq1:1:8 82 3281 S. scoparium EXP: P-SCHsc.Ubq1-1:1:11 (SEQ ID no: 83); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:11 83 2033 S. scoparium promoter EXP-SCHsc.Ubq1:1:7 84 2294 S. scoparium EXP: P-SCHsc.Ubq1-1:1:10 (SEQ ID no: 85); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:10 85 1046 S. scoparium promoter EXP-SCHsc.Ubq1:1:10 86 1795 S. scoparium EXP: P-SCHsc.Ubq1-1:1:14 (SEQ ID no: 87); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:14 87 547 S. scoparium promoter EXP-SORnu.Ubq1:1:2 88 3357 S. nutans EXP: P-SORnu.Ubq1-1:1:4 (SEQ ID no: 89); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:1 (SEQ ID no: 91) P-SORnu.Ubq1-1:1:4 89 2218 S. nutans promoter L-SORnu.Ubq1-1:1:1 90 86 S. nutans Leader I-SORnu.Ubq1-1:1:1 91 1053 S. nutans Intron EXP-SORnu.Ubq1:1:6 92 3106 S. nutans EXP: P-SORnu.Ubq1-1:1:5 (SEQ ID no: 93); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:5 93 1964 S. nutans promoter I-SORnu.Ubq1-1:1:2 94 1056 S. nutans Intron EXP-SORnu.Ubq1:1:7 95 2165 S. nutans EXP: P-SORnu.Ubq1-1:1:6 (SEQ ID no: 96); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:6 96 1023 S. nutans promoter EXP-SORnu.Ubq1:1:8 97 1866 S. nutans EXP: P-SORnu.Ubq1-1:1:7 (SEQ ID no: 98); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:7 98 724 S. nutans promoter EXP-SETit.Ubq1:1:10 99 2625 S. italica EXP: P-SETit.Ubq1-1:1:4 (SEQ ID NO: 100); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:3 (SEQ ID NO: 102) P-SETit.Ubq1-1:1:4 100 1492 S. italica promoter L-SETit.Ubq1-1:1:1 101 127 S. italica Leader I-SETit.Ubq1-1:1:3 102 1006 S. italica Intron EXP-SETit.Ubq1:1:5 103 2625 S. italica EXP: P-SETit.Ubq1-1:1:1 (SEQ ID NO: 104); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:1 104 1492 S. italica promoter I-SETit.Ubq1-1:1:2 105 1006 S. italica Intron EXP-SETit.Ubq1:1:7 106 2167 S. italica EXP: P-SETit.Ubq1-1:1:2 (SEQ ID NO: 107); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:2 107 1034 S. italica promoter EXP-SETit.Ubq1:1:6 108 1813 S. italica EXP: P-SETit.Ubq1-1:1:3 (SEQ ID NO: 109); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:3 109 680 S. italica promoter EXP-Sv.Ubq1:1:7 110 2634 S. viridis EXP: P-Sv.Ubq1-1:1:1 (SEQ ID no: 111); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:1 111 1493 S. viridis promoter L-Sv.Ubq1-1:1:2 112 127 S. viridis Leader I-Sv.Ubq1-1:1:2 113 1014 S. viridis Intron EXP-Sv.Ubq1:1:11 114 2634 S. viridis EXP: P-Sv.Ubq1-1:1:1 (SEQ ID no: 111); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:3 (SEQ ID NO: 115) I-Sv.Ubq1-1:1:3 115 1014 S. viridis Intron EXP-Sv.Ubq1:1:8 116 2176 S. viridis EXP: P-Sv.Ubq1-1:1:2 (SEQ ID no: 117); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:2 117 1035 S. viridis promoter EXP-Sv.Ubq1:1:10 118 1822 S. viridis EXP: P-Sv.Ubq1-1:1:4 (SEQ ID no: 119); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:4 119 681 S. viridis promoter EXP-Sv.Ubq1:1:12 120 1822 S. viridis EXP: P-Sv.Ubq1-1:1:3 (SEQ ID no: 121); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:3 (SEQ ID NO: 115) P-Sv.Ubq1-1:1:3 121 681 S. viridis promoter EXP-Zm.UbqM1:1:6 (allele-1) 122 1925 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:1 (SEQ ID no: 123); L-Zm.UbqM1-1:1:1 (SEQ ID no: 124); I-Zm.UbqM1-1:1:13 (SEQ ID no: 125) P-Zm.UbqM1-1:1:1 (allele-1) 123 850 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:1 (allele-1) 124 78 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:13 (allele-1) 125 997 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:10 (allele-1) 126 1925 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:1 (SEQ ID no: 123); L-Zm.UbqM1-1:1:1 (SEQ ID no: 124); I-Zm.UbqM1-1:1:17 (SEQ ID no: 127) I-Zm.UbqM1-1:1:17 (allele-1) 127 997 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:7 (allele-2) 128 1974 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:4 (SEQ ID no: 129); L-Zm.UbqM1-1:1:5 (SEQ ID no: 130); I-Zm.UbqM1-1:1:14 (SEQ ID no: 131) P-Zm.UbqM1-1:1:4 (allele-2) 129 887 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:5 (allele-2) 130 77 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:14 (allele-2) 131 1010 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:12 (allele-2) 132 1974 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:4 (SEQ ID no: 129); L-Zm.UbqM1-1:1:5 (SEQ ID no: 130); I-Zm.UbqM1-1:1:19 (SEQ ID no: 133) I-Zm.UbqM1-1:1:19 (allele-2) 133 1010 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:8 (allele-3) 134 2008 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:5 (SEQ ID no: 135); L-Zm.UbqM1-1:1:4 (SEQ ID no: 136); I-Zm.UbqM1-1:1:15 (SEQ ID no: 137) P-Zm.UbqM1-1:1:5 (allele-2) 135 877 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:4 (allele-2) 136 78 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:15 (allele-2) 137 1053 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:11 (allele-3) 138 2008 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:5 (SEQ ID no: 135); L-Zm.UbqM1-1:1:4 (SEQ ID no: 136); I-Zm.UbqM1-1:1:18 (SEQ ID no: 139) I-Zm.UbqM1-1:1:18 (allele-2) 139 1053 Z. mays subsp. Mexicana Intron EXP-Sb.Ubq4:1:2 140 1635 S. bicolor EXP: P-Sb.Ubq4-1:1:1 (SEQ ID no: 141); L-Sb.Ubq4-1:1:1 (SEQ ID no: 142); I-Sb.Ubq4-1:1:2 (SEQ ID NO: 143) P-Sb.Ubq4-1:1:1 141 401 S. bicolor promoter L-Sb.Ubq4-1:1:1 142 154 S. bicolor Leader I-Sb.Ubq4-1:1:2 143 1080 S. bicolor Intron EXP-Sb.Ubq6:1:2 144 2067 S. bicolor EXP: P-Sb.Ubq6-1:1:1 (SEQ ID no: 145); L-Sb.Ubq6-1:1:1 (SEQ ID no: 146); I-Sb.Ubq6-1:1:2 (SEQ ID NO: 147) P-Sb.Ubq6-1:1:1 145 855 S. bicolor promoter L-Sb.Ubq6-1:1:1 146 136 S. bicolor Leader I-Sb.Ubq6-1:1:2 147 1076 S. bicolor Intron EXP-Sb.Ubq6:1:3 148 2067 S. bicolor EXP: P-Sb.Ubq6-1:1:1 (SEQ ID no: 145); L-Sb.Ubq6-1:1:1 (SEQ ID no: 146); I-Sb.Ubq6-1:1:3 (SEQ ID NO: 149) I-Sb.Ubq6-1:1:3 149 1076 S. bicolor Intron EXP-Sb.Ubq7:1:2 150 2003 S. bicolor EXP: P-Sb.Ubq7-1:1:1 (SEQ ID no: 151); L-Sb.Ubq7-1:1:1 (SEQ ID NO: 152); I-Sb.Ubq7-1:1:2 (SEQ ID NO: 153) P-Sb.Ubq7-1:1:1 151 565 S. bicolor promoter L-Sb.Ubq7-1:1:1 152 77 S. bicolor Leader I-Sb.Ubq7-1:1:2 153 1361 S. bicolor Intron EXP-Cl.Ubq10 168 1790 C. lacrima-jobi EXP: P-Cl.UBQ10 (SEQ ID no: 169); L-Cl.UBQ10 (SEQ ID no: 170); I-Cl.UBQ10 (SEQ ID no: 171) P-Cl.Ubq10 169 481 C. lacrima-jobi promoter L-Cl.Ubq10 170 93 C. lacrima-jobi Leader I-Cl.Ubq10 171 1216 C. lacrima-jobi Intron

[00083] As shown in Table 1, for example, the EXP regulatory sequence designated EXP-AGRne.Ubq1:1:7 (SEQ ID NO: 1), with components isolated from A. nebulosa , includes a promoter element P-AGRne.Ubq1- 1:1:5 (SEQ ID no: 2), functionally linked to the 5'-element of the leader L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3), functionally linked to the 5'-element of the intron I- AGRne.Ubq1-1:1:3 (SEQ ID no: 4). Other EXP sequences are related similarly as shown in Table 1.

[00084] As shown in Table 1, Sequence Listing and FIG. 1-8, variants of promoter sequences from A. nebulosa, A donax, B. gracilis, M. sinensis, S. scoparium, and S. nutans have been developed containing truncated promoter fragments, for example, P-AGRne.Ubq1-1:1: 5 (SEQ ID NO:2), P-ARUdo.Ubq1-1:1:4 (SEQ ID NO:10), or other appropriate promoters from other species, and resulted in, for example, P-AGRne.Ubq1-1 :1:4 (SEQ ID no: 6) and P-ARUdo.Ubq1-1:1:5 (SEQ ID no: 14), as well as other promoter fragments.

[00085] Also listed in Table 1 are three allelic variants isolated using the same primers designed to amplify genomic DNA from Z. mays subsp. Mexicana . Allelic variants of the EXP sequences of Z. mays subsp. Mexicana contain DNA sequences that have a certain identity within various regions of other DNA sequences, but insertions, deletions and nucleotide mismatches can also be detected within each promoter, leader and/or intron of each of the EXP sequences. The EXP sequences designated EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122) and EXP-Zm.UbqM1:1:10 (SEQ ID NO: 126) represent the first allele (allele-1) of Z. mays subsp . Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction. The EXP sequences designated EXP-Zm.UbqM1:1:7 (SEQ ID NO: 128) and EXP-Zm.UbqM1:1:12 (SEQ ID NO: 132) represent the second allele (allele-2) of Z. mays subsp . Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction. The EXP sequences EXP-Zm.UbqM1:1:8 (SEQ ID NO: 134) and EXP-Zm.UbqM1:1:11 (SEQ ID NO: 138) represent the third allele (allele-3) of Z. mays subsp. Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction.

Example 2

Analysis of regulatory elements controlling GUS in maize protoplasts using GUS

amplicon expression cassettes

[00086] Maize leaf protoplasts were transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the beta-glucuronidase (GUS) transgene and compared with leaf protoplasts in which GUS expression was under the control of known constitutive promoters, in series of experiments below.

[00087] In a first set of experiments, maize cell protoplasts derived from leaf tissue were transformed as previously described with amplicons derived from the amplification of GUS gene expression cassettes containing plant expression vectors to compare transgene (GUS) expression under the control of one from the sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-AGRne.Ubq1:1:9 (SEQ ID no: 7), EXP-ARUdo.Ubq1:1:8 (SEQ ID No.: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID No.: 16), EXP-ARUdo.Ubq1:1:11 (SEQ ID No. : 20), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 26), EXP-ARUdo.Ubq2:1:9 (SEQ ID NO: 29), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 31), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 37), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 40), EXP-BOUgr.Ubq1:1:8 (SEQ ID no: 42), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 51), EXP-BOUgr.Ubq2:1:16 (SEQ ID no: 57), EXP-BOUgr.Ubq2:1:17 ( SEQ ID NO: 59), EXP-MISsi.Ubq1:1:8 (SEQ ID NO: 69), EXP-MISsi.Ubq1:1:10 (SEQ ID NO: 71), EXP-MISsi.Ubq1:1:11 (SEQ ID NO: 73), EXP-MISsi.Ubq1:1:7 (SEQ ID NO: 75), EXP-SCHsc.Ubq1:1:9 (SEQ ID NO: 77), EXP-SCHsc.Ubq1:1: 7 (SEQ ID NO: 83), EXP-SCHsc.Ubq1:1:10 (SEQ ID NO: 85), EXP-SORnu.Ubq1:1:6 (SEQ ID NO: 91), EXP-SORnu.Ubq1:1 :7 (SEQ ID No.: 94), EXP-SORnu.Ubq1:1:8 (SEQ ID No.: 96), EXP-SETit.Ubq1:1:5 (SEQ ID No.: 102), EXP-SETit.Ubq1: 1:7 (SEQ ID No.: 105), EXP-SETit.Ubq1:1:6 (SEQ ID No.: 107), EXP-Sv.Ubq1:1:7 (SEQ ID No.: 109), EXP-Sv.Ubq1 :1:8 (SEQ ID NO: 115), EXP-Sv.Ubq1:1:10 (SEQ ID NO: 117), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 121), EXP-Zm. UbqM1:1:7 (SEQ ID NO: 127), EXP-Zm.UbqM1:1:8 (SEQ ID NO: 133), Exp-Sb.Ubq4:1:2 (SEQ ID NO: 139) and Exp-Sb .Ubq6:1:2 (SEQ ID NO: 143) under the control of known constitutive promoters. Each EXP sequence containing the amplification template from which the amplicon expression cassette is derived was cloned using methods known in the art into the plant expression vector shown in Table 2 below under the heading "amplicon template". The resulting plant expression vectors contain an EXP sequence operably linked to a 5' GUS coding sequence that contains either a processable intron ("GUS-2", SEQ ID no. 154) or an adjacent GUS coding sequence ("GUS-1", SEQ ID no: 153) operably linked to the 5'-3'-UTR of T-AGRtu.nos-1:1:13 (SEQ ID no: 157) or T-Ta.Hsp17-1:1:1 (SEQ ID no: 158). Amplicons were generated using methods known to those skilled in the art using the plasmid construct templates shown in Table 2 below. Briefly, a 5' oligonucleotide primer was designed to anneal the promoter sequence, and a 3' oligonucleotide primer that anneals from the 3' end of the 3' UTR was used to amplify each expression cassette. Sequential 5' deletions were introduced into the promoter sequences constituting the expression cassettes, giving rise to different EXP sequences, by using different oligonucleotide primers that were designed to anneal at different positions within the promoter sequence constituting each amplicon template.

Table 2. Amplicons for expression in plants with the GUS gene sequence and corresponding templates for plasmid amplicon constructs, the EXP sequence encoding the GUS sequence, and the 3'-UTR used to transform maize leaf protoplasts.

Amplicon ID Amplicon matrix EXP sequence SEQID No.: GUS coding sequence 3'-UTR PCR0145942 pMON25455 EXP-Os.Act1:1:9 162 GUS-1 T-AGRtu.nos-1:1:13 PCR0145943 pMON65328 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 GUS-2 T-Ta.Hsp17-1:1:1 PCR0145935 pMON140890 EXP-AGRne.Ubq1:1:7 1 GUS-1 T-AGRtu.nos-1:1:13 PCR0145827 pMON140890 EXP-AGRne.Ubq1:1:8 5 GUS-1 T-AGRtu.nos-1:1:13 PCR0145828 pMON140890 EXP-AGRne.Ubq1:1:9 7 GUS-1 T-AGRtu.nos-1:1:13 PCR0145939 pMON140894 EXP-ARUdo.Ubq1:1:8 13 GUS-1 T-AGRtu.nos-1:1:13 PCR0145837 pMON140894 EXP-ARUdo.Ubq1:1:9 18 GUS-1 T-AGRtu.nos-1:1:13 PCR0145838 pMON140894 EXP-ARUdo.Ubq1:1:11 21 GUS-1 T-AGRtu.nos-1:1:13 PCR0145940 pMON140895 EXP-ARUdo.Ubq2:1:8 27 GUS-1 T-AGRtu.nos-1:1:13 PCR0145841 pMON140895 EXP-ARUdo.Ubq2:1:9 thirty GUS-1 T-AGRtu.nos-1:1:13 PCR0145842 pMON140895 EXP-ARUdo.Ubq2:1:10 32 GUS-1 T-AGRtu.nos-1:1:13 PCR0145936 pMON140891 EXP-BOUgr.Ubq1:1:6 38 GUS-1 T-AGRtu.nos-1:1:13 PCR0145829 pMON140891 EXP-BOUgr.Ubq1:1:7 41 GUS-1 T-AGRtu.nos-1:1:13 PCR0145831 pMON140891 EXP-BOUgr.Ubq1:1:8 43 GUS-1 T-AGRtu.nos-1:1:13 PCR0145937 pMON140892 EXP-BOUgr.Ubq2:1:14 52 GUS-1 T-AGRtu.nos-1:1:13 PCR0145833 pMON140892 EXP-BOUgr.Ubq2:1:16 58 GUS-1 T-AGRtu.nos-1:1:13 PCR0145836 pMON140892 EXP-BOUgr.Ubq2:1:17 60 GUS-1 T-AGRtu.nos-1:1:13 PCR0145898 pMON136265 EXP-MISsi.Ubq1:1:8 70 GUS-1 T-AGRtu.nos-1:1:13 PCR0145823 pMON136265 EXP-MISsi.Ubq1:1:10 72 GUS-1 T-AGRtu.nos-1:1:13 PCR0145824 pMON136265 EXP-MISsi.Ubq1:1:11 74 GUS-1 T-AGRtu.nos-1:1:13 PCR0145899 pMON136260 EXP-MISsi.Ubq1:1:7 76 GUS-1 T-AGRtu.nos-1:1:13 PCR0145894 pMON136262 EXP-SCHsc.Ubq1:1:9, 78 GUS-1 T-AGRtu.nos-1:1:13 PCR0145895 pMON136257 EXP-SCHsc.Ubq1:1:7 84 GUS-1 T-AGRtu.nos-1:1:13 PCR0145813 pMON136257 EXP-SCHsc.Ubq1:1:10 86 GUS-1 T-AGRtu.nos-1:1:13 PCR0145938 pMON140893 EXP-SORnu.Ubq1:1:6 92 GUS-1 T-AGRtu.nos-1:1:13 PCR0145839 pMON140893 EXP-SORnu.Ubq1:1:7 95 GUS-1 T-AGRtu.nos-1:1:13 PCR0145840 pMON140893 EXP-SORnu.Ubq1:1:8 97 GUS-1 T-AGRtu.nos-1:1:13 PCR0145900 pMON140877 EXP-SETit.Ubq1:1:5 103 GUS-1 T-AGRtu.nos-1:1:13 PCR0145928 pMON140877 EXP-SETit.Ubq1:1:7 106 GUS-1 T-AGRtu.nos-1:1:13 PCR0145905 pMON140877 EXP-SETit.Ubq1:1:6 108 GUS-1 T-AGRtu.nos-1:1:13 PCR0145909 pMON140878 EXP-Sv.Ubq1:1:7 110 GUS-1 T-AGRtu.nos-1:1:13 PCR0145929 pMON140878 EXP-Sv.Ubq1:1:8 116 GUS-1 T-AGRtu.nos-1:1:13 PCR0145911 pMON140878 EXP-Sv.Ubq1:1:10 118 GUS-1 T-AGRtu.nos-1:1:13 PCR0145914 pMON140881 EXP-Zm.UbqM1:1:6 122 GUS-1 T-AGRtu.nos-1:1:13 PCR0145916 pMON140883 EXP-Zm.UbqM1:1:7 128 GUS-1 T-AGRtu.nos-1:1:13 PCR0145915 pMON140882 EXP-Zm.UbqM1:1:8 134 GUS-1 T-AGRtu.nos-1:1:13 PCR0145921 pMON140887 Exp-Sb.Ubq4:1:2 140 GUS-1 T-AGRtu.nos-1:1:13 PCR0145920 pMON140886 Exp-Sb.Ubq6:1:2 144 GUS-1 T-AGRtu.nos-1:1:13

[00088] The plasmid constructs shown as amplicon templates in Table 2 served as templates for amplifying transgene expression cassettes containing the EXP sequences listed in Table 2. Control plasmids used to generate GUS transgene amplicons for comparison were generated as previously described with constitutive EXP sequences EXP-Os.Act1:1:9 (SEQ ID no: 162) and EXP-CaMV.35S-enh+Ta .Lhcb1+Os.Act1:1:1 (SEQ ID no: 161). An empty vector, not designed to express the transgene, was used as a negative control to assess background expression of GUS and luciferase.

[00089] Two plasmids were also constructed for use in co-transformation and data normalization using methods known in the art. Each plasmid contained a specific luciferase-coding sequence under the control of the constitutive EXP sequence. The plant vector pMON19437 contains an expression cassette with a constitutive promoter operably linked to the 5' intron (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 163) operably linked to the 5' coding sequence firefly ( Photinus pyralis ) luciferase (LUCIFERASE:1:3, SEQ ID no: 156) operably linked to the 5'-3'-UTR from the nopaline synthase gene of Agrobacterium tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID No: 158). The plant vector pMON63934 contains an expression cassette with the constitutive sequence EXP (EXP-CaMV.35S-enh-Lhcb1, SEQ ID no: 164) operably linked to the 5'-coding sequence of the sea sponge luciferase ( Renilla reniformis ) (CR-Ren.hRenilla Lucife -0:0:1, SEQ ID no: 157) operably linked to the 5'-3'-UTR from the Agrobacterium tumefaciens nopaline synthase gene (T-AGRtu.nos-1:1:13, SEQ ID no: 158).

[00090] Maize leaf protoplasts were transformed using a PEG-based transformation method that is well known in the art. Protoplast cells were transformed with pMON19437 and pMON63934 plasmid DNA and the amplicons shown in Table 2 and incubated overnight in total darkness. Measurements of both GUS and luciferase were performed by placing aliquots of lysed cell preparations, transformed as described previously, into two different microwell plates. One plate was used to measure GUS and the second plate was used to perform a dual luciferase assay using a dual luciferase reporter assay system (Promega Corp., Madison, WI; see, for example, Promega Notes Magazine, No: 57, 1996, p.02 ). One or two transformations were performed for each EXP sequence and average expression values were determined for each EXP sequence from several samples of each transformation experiment. Sample measurements were performed using four replications for each transformation with the EXP sequence genetic construct, or alternatively three replications for each EXP sequence amplicon in one of two transformation experiments. Values for GUS and luciferase expression levels are shown in Table 3. In this table, firefly luciferase values ( e.g. as a result of expression of pMON19437) are shown in the column labeled "FLuc" and Renilla luciferase values are listed in the column labeled "RLuc".

Table 3 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 5 7840.58 205661 EXP-Os.Act1:1:9 162 1540.25 2671.83 105417 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 12530.8 3067.08 137723 EXP-AGRne.Ubq1:1:7 1 39665 3645.83 137384 EXP-AGRne.Ubq1:1:8 5 22805.5 4183.58 140991 EXP-AGRne.Ubq1:1:9 7 5861.5 887.08 34034.3 EXP-ARUdo.Ubq1:1:8 13 26965.5 1052.33 37774.8 EXP-ARUdo.Ubq1:1:9 18 66126 3251.08 114622 EXP-ARUdo.Ubq1:1:11 21 136163 453851 EXP-ARUdo.Ubq2:1:8 27 13222.3 2203.58 72339.1 EXP-ARUdo.Ubq2:1:9 thirty 30095 6538.58 229201 EXP-ARUdo.Ubq2:1:10 32 16448.5 1842.58 65325.1 EXP-BOUgr.Ubq1:1:6 38 32544.3 2765.08 80330.8 EXP-BOUgr.Ubq1:1:7 41 3826.33 697.11 20709 EXP-BOUgr.Ubq1:1:8 43 9935.5 3372.58 110965 EXP-BOUgr.Ubq2:1:14 52 17828 1575.83 62286.8 EXP-BOUgr.Ubq2:1:16 58 54970.3 3389.08 117616 EXP-BOUgr.Ubq2:1:17 60 48601.3 7139.08 245785 EXP-MISsi.Ubq1:1:8 70 11788.3 3264.58 87751.6 EXP-MISsi.Ubq1:1:10 72 33329.5 2388.58 81000.6 EXP-MISsi.Ubq1:1:11 74 4723.75 3135.33 98059.1 EXP-MISsi.Ubq1:1:7 76 4499 3073.58 84015.1 EXP-SCHsc.Ubq1:1:9, 78 5972 1703.33 62310.6 EXP-SCHsc.Ubq1:1:7 84 24173.5 5306.08 155122 EXP-SCHsc.Ubq1:1:10 86 7260 1171.08 38698.1 EXP-SORnu.Ubq1:1:6 92 3966.5 4175.08 129365 EXP-SORnu.Ubq1:1:7 95 23375.5 616.83 25125.3 EXP-SORnu.Ubq1:1:8 97 8431.75 1630.08 55095.6 EXP-SETit.Ubq1:1:5 103 20496.5 2358.83 88695.8 EXP-SETit.Ubq1:1:7 106 75728.5 4723.08 185224 EXP-SETit.Ubq1:1:6 108 44148.3 4962.08 161216 EXP-Sv.Ubq1:1:7 110 15043.8 1888.33 74670.6 EXP-Sv.Ubq1:1:8 116 31997.8 3219.83 113787 EXP-Sv.Ubq1:1:10 118 38952.8 7011.33 220209 EXP-Zm.UbqM1:1:6 122 30528.3 2453.58 90113.1 EXP-Zm.UbqM1:1:8 134 34986.3 2553.78 105725 Exp-Sb.Ubq4:1:2 140 9982.25 2171.58 72593.8 Exp-Sb.Ubq6:1:2 144 33689 3879.58 114710

[00091] To compare the relative activity of each EXP sequence, GUS values were expressed as the ratio of GUS activity to luciferase activity and normalized to the expression levels observed for EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+ Ta.Lhcb1+Os.Act1:1:1. Table 4 below shows GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 controlled expression in maize protoplasts . Table 5 below shows GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 controlled expression in maize protoplasts .

Table 4. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 (SEQ ID NO: 161) in maize protoplasts.

EXP sequence SEQID No.: GUS/FLuc versus EXP-CaMV.35S-enh+Ta.Lhcb1+
Os.Act1:1:1 GUS/RLuc vs. EXP-CaMV.35S-enh+Ta.Lhcb1+
Os.Act1:1:1 EXP-Os.Act1:1:9 162 0.14 0.16 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 1 1 EXP-AGRne.Ubq1:1:7 1 2.66 3.17 EXP-AGRne.Ubq1:1:8 5 1.33 1.78 EXP-AGRne.Ubq1:1:9 7 1.62 1.89 EXP-ARUdo.Ubq1:1:8 13 6.27 7.85 EXP-ARUdo.Ubq1:1:9 18 4.98 6.34 EXP-ARUdo.Ubq1:1:11 21 3.3 EXP-ARUdo.Ubq2:1:8 27 1.47 2.01 EXP-ARUdo.Ubq2:1:9 thirty 1.13 1.44 EXP-ARUdo.Ubq2:1:10 32 2.18 2.77 EXP-BOUgr.Ubq1:1:6 38 2.88 4.45 EXP-BOUgr.Ubq1:1:7 41 1.34 2.03 EXP-BOUgr.Ubq1:1:8 43 0.72 0.98 EXP-BOUgr.Ubq2:1:14 52 2.77 3.15 EXP-BOUgr.Ubq2:1:16 58 3.97 5.14 EXP-BOUgr.Ubq2:1:17 60 1.67 2.17 EXP-MISsi.Ubq1:1:8 70 0.88 1.48 EXP-MISsi.Ubq1:1:10 72 3.42 4.52 EXP-MISsi.Ubq1:1:11 74 0.37 0.53 EXP-MISsi.Ubq1:1:7 76 0.36 0.59 EXP-SCHsc.Ubq1:1:9, 78 0.86 1.05 EXP-SCHsc.Ubq1:1:7 84 1.12 1.71 EXP-SCHsc.Ubq1:1:10 86 1.52 2.06 EXP-SORnu.Ubq1:1:6 92 0.23 0.34 EXP-SORnu.Ubq1:1:7 95 9.28 10.23 EXP-SORnu.Ubq1:1:8 97 1.27 1.68 EXP-SETit.Ubq1:1:5 103 2.13 2.54 EXP-SETit.Ubq1:1:7 106 3.92 4.49 EXP-SETit.Ubq1:1:6 108 2.18 3.01 EXP-Sv.Ubq1:1:7 110 1.95 2.21 EXP-Sv.Ubq1:1:8 116 2.43 3.09 EXP-Sv.Ubq1:1:10 118 1.36 1.94 EXP-Zm.UbqM1:1:6 122 3.05 3.72 EXP-Zm.UbqM1:1:8 134 3.35 3.64 Exp-Sb.Ubq4:1:2 140 1.13 1.51 Exp-Sb.Ubq6:1:2 144 2.13 3.23

Table 5. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 162) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS/FLuc vs. EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-Os.Act1:1:9 EXP-Os.Act1:1:9 162 1 1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 7.09 6.23 EXP-AGRne.Ubq1:1:7 1 18.87 19.76 EXP-AGRne.Ubq1:1:8 5 9.46 11.07 EXP-AGRne.Ubq1:1:9 7 11.46 11.79 EXP-ARUdo.Ubq1:1:8 13 44.45 48.86 EXP-ARUdo.Ubq1:1:9 18 35.28 39.48 EXP-ARUdo.Ubq1:1:11 21 20.53 EXP-ARUdo.Ubq2:1:8 27 10.41 12.51 EXP-ARUdo.Ubq2:1:9 thirty 7.98 8.99 EXP-ARUdo.Ubq2:1:10 32 15.49 17.23 EXP-BOUgr.Ubq1:1:6 38 20.42 27.73 EXP-BOUgr.Ubq1:1:7 41 9.52 12.65 EXP-BOUgr.Ubq1:1:8 43 5.11 6.13 EXP-BOUgr.Ubq2:1:14 52 19.63 19.59 EXP-BOUgr.Ubq2:1:16 58 28.14 31.99 EXP-BOUgr.Ubq2:1:17 60 11.81 13.53 EXP-MISsi.Ubq1:1:8 70 6.26 9.19 EXP-MISsi.Ubq1:1:10 72 24.21 28.16 EXP-MISsi.Ubq1:1:11 74 2.61 3.3 EXP-MISsi.Ubq1:1:7 76 2.54 3.67 EXP-SCHsc.Ubq1:1:9, 78 6.08 6.56 EXP-SCHsc.Ubq1:1:7 84 7.9 10.67 EXP-SCHsc.Ubq1:1:10 86 10.75 12.84 EXP-SORnu.Ubq1:1:6 92 1.65 2.1 EXP-SORnu.Ubq1:1:7 95 65.74 63.67 EXP-SORnu.Ubq1:1:8 97 8.97 10.47 EXP-SETit.Ubq1:1:5 103 15.07 15.82 EXP-SETit.Ubq1:1:7 106 27.81 27.98 EXP-SETit.Ubq1:1:6 108 15.43 18.74 EXP-Sv.Ubq1:1:7 110 13.82 13.79 EXP-Sv.Ubq1:1:8 116 17.24 19.25 EXP-Sv.Ubq1:1:10 118 9.64 12.11 EXP-Zm.UbqM1:1:6 122 21.58 23.19 EXP-Zm.UbqM1:1:8 134 23.76 22.65 Exp-Sb.Ubq4:1:2 140 7.97 9.41 Exp-Sb.Ubq6:1:2 144 15.06 20.1

[00092] As can be seen from Tables 9 and 10, all EXP sequences were capable of expressing the GUS transgene in maize cells. The mean GUS expression was higher for all EXP sequences compared to EXP-Os.Act1:1:9. EXP sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-AGRne.Ubq1:1:9 (SEQ ID no: 7), EXP-ARUdo.Ubq1:1:8 (SEQ ID No.: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID No.: 18), EXP-ARUdo.Ubq1:1:11 (SEQ ID No. : 21), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 27), EXP-ARUdo.Ubq2:1:9 (SEQ ID NO: 30), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 32), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:16 (SEQ ID no: 58), EXP-BOUgr.Ubq2:1:17 (SEQ ID no: 60), EXP-MISsi.Ubq1:1:10 ( SEQ ID NO: 72), EXP-SCHsc.Ubq1:1:7 (SEQ ID NO: 84), EXP-SCHsc.Ubq1:1:10 (SEQ ID NO: 86), EXP-SORnu.Ubq1:1:7 (SEQ ID NO: 95), EXP-SORnu.Ubq1:1:8 (SEQ ID NO: 97), EXP-SETit.Ubq1:1:5 (SEQ ID NO: 103), EXP-SETit.Ubq1:1: 7 (SEQ ID NO: 106), EXP-SETit.Ubq1:1:6 (SEQ ID NO: 108), EXP-Sv.Ubq1:1:7 (SEQ ID NO: 110), EXP-Sv.Ubq1:1 :8 (SEQ ID NO: 116), EXP-Sv.Ubq1:1:10 (SEQ ID NO: 118), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122), EXP-Zm.UbqM1: 1:8 (SEQ ID no: 134), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Sb.Ubq6:1:2 (SEQ ID no: 144) showed GUS expression levels higher than than EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1.

[00093] In a second set of experiments, the GUS gene amplicon expression cassette comprising the sequence EXP EXP-Zm.UbqM1:1:7 (SEQ ID NO: 128) was compared with control amplicons, PCR0145942 (EXP-Os.Act1:1:9, SEQ ID no: 162) and PCR0145944 (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 161) in relation to GUS expression. GUS expression under the control of the EXP sequence EXP-Zm.UbqM1:1:7 was higher than in the case of two controls. Table 6 below shows the average GUS and luciferase values determined for each amplicon. Table 7 below shows GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in maize protoplasts .

Table 6 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 1512.25 11333.75 190461.00 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 41176.50 13885.75 330837.25 EXP-Zm.UbqM1:1:7 128 79581.50 15262.50 330755.75

Table 7. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 1512.25 11333.75 190461.00 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 41176.50 13885.75 330837.25 EXP-Zm.UbqM1:1:7 128 79581.50 15262.50 330755.75

[00094] The efficacy of regulatory elements controlling GUS expression from amplicons can be studied in a similar manner in sugarcane leaf protoplasts. For example, sugar cane protoplasts can be transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the GUS transgene and compared to a leaf protoplast in which GUS expression is controlled by known constitutive promoters.

Example 3

Analysis of Regulatory Elements Controlling GUS in Wheat Protoplasts Using GUS Amplicon Expression Cassettes

[00095] Wheat leaf protoplasts were transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the GUS transgene and compared to a leaf protoplast in which GUS expression was under the control of known constitutive promoters.

[00096] Wheat protoplast cells derived from leaf tissues were transformed using methods known in the art with amplicons derived from the amplification of GUS gene expression cassettes containing plant expression vectors to compare transgene (GUS) expression under control EXP the sequences listed in Table 3 with that under the control of known constitutive promoters as described in the previous example (Example 2), using the same GUS gene amplicon expression cassettes as used previously for the maize assay in Example 2. Amplicons the control GUS expression cassette and the luciferase gene plasmids used to transform wheat protoplasts were the same as those presented in the previous example and those shown earlier in Table 3 of Example 2. Similarly, negative controls were used to determine the background activity of GUS and luciferase, as described earlier. Wheat leaf protoplasts were transformed using the PEG-based transformation method as previously described in Example 2. Table 8 shows the mean GUS and LUC activity observed in cell protoplasts from transformed wheat leaves, and Tables 9 and 10 show the normalized GUS/FLuc and GUS/RLuc ratios of expression in wheat protoplasts relative to expression under constitutive EXP controls.

Table 8 Mean GUS and luciferase activity in cell protoplasts from transformed wheat leaves.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 262.56 1109.78 61422.1 EXP-Os.Act1:1:9 162 2976.33 730.11 53334.8 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 29299.3 741.78 50717.4 EXP-AGRne.Ubq1:1:7 1 27078.3 754.44 44235.8 EXP-AGRne.Ubq1:1:8 5 22082.7 958.11 55774.8 EXP-AGRne.Ubq1:1:9 7 13882.7 699.78 49273.4 EXP-ARUdo.Ubq1:1:8 13 65628 791.44 56358.8 EXP-ARUdo.Ubq1:1:9 18 87615 801.44 53246.4 EXP-ARUdo.Ubq1:1:11 21 19224.3 143.44 14104.1 EXP-ARUdo.Ubq2:1:8 27 25453.3 835.11 57679.4 EXP-ARUdo.Ubq2:1:9 thirty 26720.7 702.44 47455.4 EXP-ARUdo.Ubq2:1:10 32 37089.3 859.11 57814.4 EXP-BOUgr.Ubq1:1:6 38 35146 995.44 64418.8 EXP-BOUgr.Ubq1:1:7 41 18077 857.78 55793.4 EXP-BOUgr.Ubq1:1:8 43 11723.7 938.44 59362.1 EXP-BOUgr.Ubq2:1:14 52 38109.3 875.11 58048.1 EXP-BOUgr.Ubq2:1:16 58 37384 860.44 52447.8 EXP-BOUgr.Ubq2:1:17 60 24090.7 968.78 53057.8 EXP-MISsi.Ubq1:1:8 70 16456.7 1021.78 61684.1 EXP-MISsi.Ubq1:1:10 72 42816.7 839.78 46688.1 EXP-MISsi.Ubq1:1:11 74 20625.7 987.78 61842.1 EXP-MISsi.Ubq1:1:7 76 4913.67 764.78 64720.1 EXP-SCHsc.Ubq1:1:9, 78 9726 937.11 54725.4 EXP-SCHsc.Ubq1:1:7 84 13374.7 1112.44 73815.4 EXP-SCHsc.Ubq1:1:10 86 13650 936.78 62242.1 EXP-SORnu.Ubq1:1:6 92 8188.17 753.83 50572.5 EXP-SORnu.Ubq1:1:7 95 83233.7 854.44 54410.1 EXP-SORnu.Ubq1:1:8 97 21904.7 1011.83 60852 EXP-SETit.Ubq1:1:5 103 39427.7 908.78 57463.1 EXP-SETit.Ubq1:1:7 106 108091 809.44 49330.4 EXP-SETit.Ubq1:1:6 108 58703 809.11 46110.1 EXP-Sv.Ubq1:1:7 110 29330 684.11 43367.1 EXP-Sv.Ubq1:1:8 116 53359 698.11 40076.4 EXP-Sv.Ubq1:1:10 118 49122.7 901.44 53180.8 EXP-Zm.UbqM1:1:6 122 37268 945.78 54088.1 EXP-Zm.UbqM1:1:8 134 51408 677.78 47297.4 Exp-Sb.Ubq4:1:2 140 35660.3 1114.11 62591.1 Exp-Sb.Ubq6:1:2 144 27543 915.11 57826.4

Table 9. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 (SEQ ID NO: 161) in wheat protoplasts.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 262.56 1109.78 61422.1 EXP-Os.Act1:1:9 162 2976.33 730.11 53334.8 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 29299.3 741.78 50717.4 EXP-AGRne.Ubq1:1:7 1 27078.3 754.44 44235.8 EXP-AGRne.Ubq1:1:8 5 22082.7 958.11 55774.8 EXP-AGRne.Ubq1:1:9 7 13882.7 699.78 49273.4 EXP-ARUdo.Ubq1:1:8 13 65628 791.44 56358.8 EXP-ARUdo.Ubq1:1:9 18 87615 801.44 53246.4 EXP-ARUdo.Ubq1:1:11 21 19224.3 143.44 14104.1 EXP-ARUdo.Ubq2:1:8 27 25453.3 835.11 57679.4 EXP-ARUdo.Ubq2:1:9 thirty 26720.7 702.44 47455.4 EXP-ARUdo.Ubq2:1:10 32 37089.3 859.11 57814.4 EXP-BOUgr.Ubq1:1:6 38 35146 995.44 64418.8 EXP-BOUgr.Ubq1:1:7 41 18077 857.78 55793.4 EXP-BOUgr.Ubq1:1:8 43 11723.7 938.44 59362.1 EXP-BOUgr.Ubq2:1:14 52 38109.3 875.11 58048.1 EXP-BOUgr.Ubq2:1:16 58 37384 860.44 52447.8 EXP-BOUgr.Ubq2:1:17 60 24090.7 968.78 53057.8 EXP-MISsi.Ubq1:1:8 70 16456.7 1021.78 61684.1 EXP-MISsi.Ubq1:1:10 72 42816.7 839.78 46688.1 EXP-MISsi.Ubq1:1:11 74 20625.7 987.78 61842.1 EXP-MISsi.Ubq1:1:7 76 4913.67 764.78 64720.1 EXP-SCHsc.Ubq1:1:9, 78 9726 937.11 54725.4 EXP-SCHsc.Ubq1:1:7 84 13374.7 1112.44 73815.4 EXP-SCHsc.Ubq1:1:10 86 13650 936.78 62242.1 EXP-SORnu.Ubq1:1:6 92 8188.17 753.83 50572.5 EXP-SORnu.Ubq1:1:7 95 83233.7 854.44 54410.1 EXP-SORnu.Ubq1:1:8 97 21904.7 1011.83 60852 EXP-SETit.Ubq1:1:5 103 39427.7 908.78 57463.1 EXP-SETit.Ubq1:1:7 106 108091 809.44 49330.4 EXP-SETit.Ubq1:1:6 108 58703 809.11 46110.1 EXP-Sv.Ubq1:1:7 110 29330 684.11 43367.1 EXP-Sv.Ubq1:1:8 116 53359 698.11 40076.4 EXP-Sv.Ubq1:1:10 118 49122.7 901.44 53180.8 EXP-Zm.UbqM1:1:6 122 37268 945.78 54088.1 EXP-Zm.UbqM1:1:8 134 51408 677.78 47297.4 Exp-Sb.Ubq4:1:2 140 35660.3 1114.11 62591.1 Exp-Sb.Ubq6:1:2 144 27543 915.11 57826.4

Table 10. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 162) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS/FLuc vs. EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-Os.Act1:1:9 EXP-Os.Act1:1:9 162 1 1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 9.69 10.35 EXP-AGRne.Ubq1:1:7 1 8.8 10.97 EXP-AGRne.Ubq1:1:8 5 5.65 7.09 EXP-AGRne.Ubq1:1:9 7 4.87 5.05 EXP-ARUdo.Ubq1:1:8 13 20.34 20.87 EXP-ARUdo.Ubq1:1:9 18 26.82 29.49 EXP-ARUdo.Ubq1:1:11 21 32.88 24.43 EXP-ARUdo.Ubq2:1:8 27 7.48 7.91 EXP-ARUdo.Ubq2:1:9 thirty 9.33 10.09 EXP-ARUdo.Ubq2:1:10 32 10.59 11.5 EXP-BOUgr.Ubq1:1:6 38 8.66 9.78 EXP-BOUgr.Ubq1:1:7 41 5.17 5.81 EXP-BOUgr.Ubq1:1:8 43 3.06 3.54 EXP-BOUgr.Ubq2:1:14 52 10.68 11.76 EXP-BOUgr.Ubq2:1:16 58 10.66 12.77 EXP-BOUgr.Ubq2:1:17 60 6.1 8.14 EXP-MISsi.Ubq1:1:8 70 3.95 4.78 EXP-MISsi.Ubq1:1:10 72 12.51 16.43 EXP-MISsi.Ubq1:1:11 74 5.12 5.98 EXP-MISsi.Ubq1:1:7 76 1.58 1.36 EXP-SCHsc.Ubq1:1:9, 78 2.55 3.18 EXP-SCHsc.Ubq1:1:7 84 2.95 3.25 EXP-SCHsc.Ubq1:1:10 86 3.57 3.93 EXP-SORnu.Ubq1:1:6 92 2.66 2.9 EXP-SORnu.Ubq1:1:7 95 23.9 27.41 EXP-SORnu.Ubq1:1:8 97 5.31 6.45 EXP-SETit.Ubq1:1:5 103 10.64 12.3 EXP-SETit.Ubq1:1:7 106 32.76 39.26 EXP-SETit.Ubq1:1:6 108 17.8 22.81 EXP-Sv.Ubq1:1:7 110 10.52 12.12 EXP-Sv.Ubq1:1:8 116 18.75 23.86 EXP-Sv.Ubq1:1:10 118 13.37 16.55 EXP-Zm.UbqM1:1:6 122 9.67 12.35 EXP-Zm.UbqM1:1:8 134 18.61 19.48 Exp-Sb.Ubq4:1:2 140 7.85 10.21 Exp-Sb.Ubq6:1:2 144 7.38 8.54

[00097] As can be seen from Tables 9 and 10 above, all EXP sequences were capable of expressing the GUS transgene in wheat cells. All EXP sequences expressed GUS at a higher level than EXP-Os.Act1:1:9 in wheat cells. EXP sequences EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP-ARUdo.Ubq1:1:11 (SEQ ID no: 21), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 32), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:16 (SEQ ID no. : 58), EXP-BOUgr.Ubq2:1:17 (SEQ ID no: 60), EXP-MISsi.Ubq1:1:10 (SEQ ID no: 72), EXP-SORnu.Ubq1:1:7 (SEQ ID No.: 95), EXP-SETit.Ubq1:1:5 (SEQ ID No.: 103), EXP-SETit.Ubq1:1:7 (SEQ ID No.: 106), EXP-SETit.Ubq1:1:6 (SEQ ID NO: 108), EXP-Sv.Ubq1:1:7 (SEQ ID NO: 110), EXP-Sv.Ubq1:1:8 (SEQ ID NO: 116), EXP-Sv.Ubq1:1:10 ( SEQ ID NO: 118), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122) and EXP-Zm.UbqM1:1:8 (SEQ ID NO: 134) showed GUS expression levels equal to or greater than levels of GUS expression under the control of EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 in wheat cells.

[00098] In a second series of experiments, a GUS gene amplicon expression cassette comprising EXP-ARUdo.Ubq1:1:11 (SEQ ID no: 21) was compared with controls EXP-Os.Act1:1:9 (SEQ ID no: 162 ) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID no: 161). Table 11 below shows the average GUS and luciferase values determined for each amplicon. Table 12 below shows GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in wheat protoplasts.

Table 11 Mean GUS and luciferase activity in cell protoplasts from transformed wheat leaves.

EXP sequence SEQID No.: GUS RLuc Blank vector 20.75 187112.50 EXP-Os.Act1:1:9 162 1234.00 176970.50 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 12883.50 119439.00 EXP-ARUdo.Ubq1:1:11 21 30571.50 135037.50

Table 12 GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/RLuc in relation to EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.
act1:1:1 EXP-Os.Act1:1:9 162 1.00 0.06 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 15.47 1.00 EXP-ARUdo.Ubq1:1:11 21 32.47 2.10

[00099] As can be seen previously from Table 12, GUS expression under the EXP-ARUdo.Ubq1:1:11 control (SEQ ID NO: 21) was higher than the two constitutive controls EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1.

Example 4

Analysis of Regulatory Elements Controlling GUS in Maize and Wheat Protoplasts

[000100] Maize and wheat leaf protoplasts were transformed with plant expression vectors containing an EXP sequence that controls the expression of the beta-glucuronidase (GUS) transgene and compared with GUS expression in leaf protoplasts in which GUS expression was controlled by known constitutive promoters.

[000101] Expression of the transgene under the control of EXP-Cl.Ubq10 (SEQ ID no: 168) was compared with expression under the control of known constitutive promoters. The above EXP sequences were cloned into plant expression vectors as shown in Table 13 below to obtain vectors in which the EXP sequence is operably linked to a 5'-GUS reporter that contained a processable intron (referred to as GUS-2, SEQ ID NO: 160), derived from the potato light-inducible tissue-specific ST-LS1 gene (GenBank Accession: X04753), or an adjacent GUS coding sequence (GUS-1, SEQ ID NO: 159) that has been operably linked to the 5'-3'-UTR from the nopaline synthase gene A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 161) or wheat Hsp17 gene (T-Ta.Hsp17-1:1:1, SEQ ID no: 162).

Table 13 Plant expression plasmid construct with the GUS gene and corresponding EXP sequence encoding the GUS sequence and 3'-UTR used to transform maize leaf protoplasts. "SEQ ID NO:" refers to a given EXP sequence.

EXP sequence SEQID No.: GUS/RLuc in relation to EXP-Os.Act1:1:9 GUS/RLuc vs. EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-Os.Act1:1:9 162 1.00 0.06 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 15.47 1.00 EXP-ARUdo.Ubq1:1:11 21 32.47 2.10

[000102] Two plasmids were also constructed for use in co-transformation and data normalization using methods known in the art. Each plasmid contained a specific luciferase-coding sequence under the control of the constitutive EXP sequence. The plant vector pMON19437 contains an expression cassette with a constitutive promoter operably linked to the 5' intron (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 163) operably linked to the 5' coding sequence firefly ( Photinus pyralis ) luciferase (LUCIFERASE:1:3, SEQ ID no: 156) operably linked to the 5'-3'-UTR from the nopaline synthase gene of Agrobacterium tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID No: 158). The plant vector pMON63934 contains an expression cassette with the constitutive sequence EXP (EXP-CaMV.35S-enh-Lhcb1, SEQ ID no: 164) operably linked to the 5'-coding sequence of the sea sponge luciferase ( Renilla reniformis ) (CR-Ren.hRenilla Lucife -0:0:1, SEQ ID no: 157) operably linked to the 5'-3'-UTR from the Agrobacterium tumefaciens nopaline synthase gene (T-AGRtu. nos-1:1:13, SEQ ID no: 158).

[000103] Maize leaf protoplasts were transformed using a PEG-based transformation method that is well known in the art. Protoplast cells were transformed with pMON19437 and pMON63934 plasmid DNA and the plasmids shown in Table 13 and incubated overnight in total darkness. Both GUS and luciferase measurements were performed in a similar manner as described in Example 2 previously. One or two transformations were performed for each EXP sequence and average expression values were determined for each EXP sequence from several samples of each transformation experiment. Sample measurements were performed using four replications for each transformation with the EXP sequence genetic construct, or alternatively three replications for each EXP sequence genetic construct in one of two transformation experiments. Values for GUS and luciferase expression levels are shown in Table 14. In this table, firefly luciferase values ( e.g. as a result of expression of pMON19437) are shown in the column labeled "FLuc" and Renilla luciferase values are listed in the column labeled "RLuc".

Table 14 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 83997.3 80983 61619 EXP-CaMV.35S-
enh+Ta.Lhcb1+Os.Act1:1:1 161 248832 83589.8 72064.3 EXP-Cl.Ubq10 168 30790.8 65807.5 34846.3

[000104] Table 15 below shows GUS/FLuc and GUS/RLuc expression ratios normalized to expression under control of EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1, in maize protoplasts.

Table 15 GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/Fluc normalized with respect to EXP-Os.Act1:1:9 GUS/Rluc normalized with respect to EXP-Os.Act1:1:9 GUS/Fluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 GUS/Rluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-Os.Act1:1:9 162 1.00 1.00 0.35 0.39 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 2.87 2.53 1.00 1.00 EXP-Cl.Ubq10 168 0.45 0.65 0.16 0.26

[000105] As can be seen from Table 15 above, EXP-Cl.Ubq10 (SEQ ID NO: 168) was able to express GUS, but at a lower level than both constitutive controls.

[000106] The plasmids shown earlier in Table 13 were also used to transform wheat leaf protoplast cells in a similar manner to the corn leaf protoplasts described previously. Mean GUS and luciferase values are presented in Table 16 below. Table 17 below shows GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in maize protoplasts.

Table 16 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 134145 1076.67 6858.67 EXP-Cl.Ubq10 168 104669 888.67 4516

Table 17. GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/Fluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 GUS/Rluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 1.00 1.00 EXP-Cl.Ubq10 168 0.95 1.19

[000107] As can be seen from Table 17 above, EXP-Cl.Ubq10 (SEQ ID NO: 168) expressed GUS at a similar level as EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID NO: : 160) in wheat protoplast cells.

Example 5

Analysis of regulatory elements controlling GUS in transgenic maize .

[000108] Corn plants were transformed with plant expression vectors containing EXP sequences that control the expression of the GUS transgene, and the resulting plants were analyzed for GUS protein expression. The ubiquitin EXP sequences were cloned into plant binary transformation plasmid constructs using methods known in the art.

[000109] The resulting plant expression vectors contain the right border region from A. tumefaciens , the first expression cassette for analysis of the EXP sequence operably linked to the GUS coding sequence, which has the processable GUS-2 intron described previously, operably linked to 5'-3' -UTR from rice lipid transfer protein gene (T-Os.LTP-1:1:1, SEQ ID NO: 159); a second transgene selection cassette, used to select transformed plant cells, confers resistance to the herbicide glyphosate (under the control of the rice actin 1 promoter) and the left border region from A. tumefaciens . The resulting plasmids were used to transform maize plants. Table 18 lists the plasmid designations, EXP sequences, and SEQ ID Nos., which are also described in Table 1.

Table 18. Binary plasmids for plant transformation and associated EXP sequences.

Plasmid construct EXP sequence SEQID No.: pMON140869 EXP-AGRne.Ubq1:1:7 1 pMON140870 EXP-AGRne.Ubq1:1:8 5 pMON142650 EXP-ARUdo.Ubq1:1:8 13 pMON142651 EXP-ARUdo.Ubq1:1:9 18 pMON142652 EXP-ARUdo.Ubq2:1:8 27 pMON142653 EXP-ARUdo.Ubq2:1:9 thirty pMON140871 EXP-BOUgr.Ubq1:1:6 38 pMON140872 EXP-BOUgr.Ubq1:1:7 41 pMON140873 EXP-BOUgr.Ubq2:1:14 52 pMON140874 EXP-BOUgr.Ubq2:1:15 55 pMON142887 EXP-MISsi.Ubq1:1:7 76 pMON140875 EXP-SORnu.Ubq1:1:6 92 pMON140876 EXP-SORnu.Ubq1:1:7 95 pMON132037 EXP-SETit.Ubq1:1:10 99 pMON131958 EXP-Sv.Ubq1:1:11 114 pMON131959 EXP-Sv.Ubq1:1:12 120 pMON131961 EXP-Zm.UbqM1:1:10 126 pMON131963 EXP-Zm.UbqM1:1:12 132 pMON131962 EXP-Zm.UbqM1:1:11 138 pMON132932 EXP-Sb.Ubq4:1:2 140 pMON132931 EXP-Sb.Ubq6:1:3 148 pMON132974 EXP-Sb.Ubq7:1:2 150 pMON142738 EXP-Cl.Ubq10 168

[000110] Plants were transformed by Agrobacterium- mediated transformation, for example, as described in US Patent Application Publication 2009/0138985.

[000111] GUS histochemical analysis was used to qualitatively analyze the expression of transformed plants. Whole tissue sections were incubated with GUS staining solution X-Gluc (5-bromo-4-chloro-3-indolyl-beta-glucuronide) (1 mg/ml) for an appropriate period of time, washed and checked visually for blue staining. GUS activity was qualitatively determined by direct visual or microscopic examination using selected plant organs and tissues. R ₀ plants were tested for expression in roots and leaves, as well as anther, silk and developing seeds and ovary, 21 days after pollination (21 DPO).

[000112] For quantitative analysis, total protein was extracted from selected tissues of transformed maize plants. One microgram of total protein was used with the fluorogenic substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) in a total reaction volume of 50 μl. The reaction product, 4-methylumbelliferone (4-MU), fluoresces to the maximum at high pH values, where the hydroxyl group is ionized. The addition of an alkaline sodium carbonate solution simultaneously stops the reaction and adjusts the pH to quantify the fluorescent product. Fluorescence was measured at an excitation wavelength of 365 nm, emission at 445 nm using a Fluoromax-3 (Horiba; Kyoto, Japan) with a Micromax Reader, with the slit width set to 2 nm for excitation and 3 nm for emission.

[000113] The average expression of GUS in R ₀ observed for each transformation is presented in tables 19 and 20 below.

Table 19 Average GUS expression in R ₀ in root and leaf tissues.

EXP sequence SEQID No.: V3 root V4 root V7 root VT root V3 sheet V4 sheet V7 sheet VT sheet EXP-AGRne.Ubq1:1:7 1 16 25 14 49 60 48 EXP-AGRne.Ubq1:1:8 5 13 20 22 38 38 52 EXP-ARUdo.Ubq1:1:8 13 18 34 89 117 48 106 EXP-ARUdo.Ubq1:1:9 18 19 20 68 105 33 69 EXP-ARUdo.Ubq2:1:8 27 14 19 27 58 57 47 EXP-ARUdo.Ubq2:1:9 thirty 14 15 25 40 38 40 EXP-BOUgr.Ubq1:1:6 38 12 28 16 43 46 27 EXP-BOUgr.Ubq1:1:7 41 14 24 114 51 48 48 EXP-BOUgr.Ubq2:1:14 52 17 13 28 46 33 41 EXP-BOUgr.Ubq2:1:15 55 eleven 67 36 86 72 36 EXP-MISsi.Ubq1:1:7 76 17 28 13 18 12 18 EXP-SORnu.Ubq1:1:6 92 14 45 33 44 64 55 EXP-SORnu.Ubq1:1:7 95 eleven 18 20 31 36 48 EXP-SETit.Ubq1:1:10 99 0 29 57 58 37 46 EXP-Sv.Ubq1:1:11 114 nd nd 9 20 55 29 EXP-Sv.Ubq1:1:12 120 63 0 28 184 27 16 EXP-Zm.UbqM1:1:10 126 0 237 18 221 272 272 EXP-Zm.UbqM1:1:12 132 0 21 43 234 231 196 EXP-Zm.UbqM1:1:11 138 124 103 112 311 369 297 EXP-Sb.Ubq4:1:2 140 125 0 95 233 150 88 EXP-Sb.Ubq6:1:3 148 154 13 128 53 39 55 EXP-Sb.Ubq7:1:2 150 37 22 18 165 89 177 EXP-Cl.Ubq10 168 61 67 32 111 58 115

Table 20. Average expression of GUS in R ₀ in reproductive organs of corn (anther, silk) and developing seeds (ovary and endosperm).

EXP sequence SEQID No.: VT boot VT/R1 silk 21 dpo ovary 21 dpo endosperm EXP-AGRne.Ubq1:1:7 1 149 36 59 59 EXP-AGRne.Ubq1:1:8 5 73 66 33 58 EXP-ARUdo.Ubq1:1:8 13 321 253 177 355 EXP-ARUdo.Ubq1:1:9 18 242 268 97 266 EXP-ARUdo.Ubq2:1:8 27 104 99 79 157 EXP-ARUdo.Ubq2:1:9 thirty 78 71 82 139 EXP-BOUgr.Ubq1:1:6 38 58 250 43 63 EXP-BOUgr.Ubq1:1:7 41 58 77 40 49 EXP-BOUgr.Ubq2:1:14 52 236 377 48 137 EXP-BOUgr.Ubq2:1:15 55 203 134 47 180 EXP-MISsi.Ubq1:1:7 76 24 16 29 32 EXP-SORnu.Ubq1:1:6 92 361 80 37 94 EXP-SORnu.Ubq1:1:7 95 195 114 20 55 EXP-SETit.Ubq1:1:10 99 132 85 50 63 EXP-Sv.Ubq1:1:11 114 217 3 45 92 EXP-Sv.Ubq1:1:12 120 120 21 49 112 EXP-Zm.UbqM1:1:10 126 261 506 403 376 EXP-Zm.UbqM1:1:12 132 775 362 253 247 EXP-Zm.UbqM1:1:11 138 551 452 234 302 EXP-Sb.Ubq4:1:2 140 213 0 25 79 EXP-Sb.Ubq6:1:3 148 295 87 51 61 EXP-Sb.Ubq7:1:2 150 423 229 274 90 EXP-Cl.Ubq10 168 237 82 91 210

[000114] In R ₀ maize, leaf and root GUS expression levels differed among ubiquitin EXP sequences. Although all EXP sequences showed the ability to control GUS transgene expression in stably transformed plants, each EXP sequence showed a unique expression pattern compared to the others. For example, EXP sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP-ARUdo.Ubq2:1:8 (SEQ ID no: 27), EXP-ARUdo.Ubq2:1:9 (SEQ ID no: 30), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-BOUgr.Ubq2:1:14 ( SEQ ID no: 52), EXP-BOUgr.Ubq2:1:15 (SEQ ID no: 55), EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76), EXP-SORnu.Ubq1:1:6 (SEQ ID NO: 92), EXP-SORnu.Ubq1:1:7 (SEQ ID NO: 95), EXP-SETit.Ubq1:1:10 (SEQ ID NO: 99), EXP-Sv.Ubq1:1: 11 (SEQ ID NO: 114), EXP-Zm.UbqM1:1:12 (SEQ ID NO: 132) and EXP-Sb.Ubq7:1:2 (SEQ ID NO: 150) showed lower levels of GUS expression in the root at V3 and V7 stages of development in relation to EXP-Sv.Ubq1:1:12 (SEQ ID no: 120), EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1 :11 (SEQ ID no: 138), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Sb.Ubq6:1:3 (SEQ ID no: 148). Higher levels of GUS expression were observed at later stages of root development (VT) for EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP -BOUgr.Ubq1:1:7 (SEQ ID NO: 41), EXP-Zm.UbqM1:1:11 (SEQ ID NO: 138) and EXP-Sb.Ubq6:1:3 (SEQ ID NO: 148). Expression in roots under the control of EXP-Zm. UbqM1:1:10 (SEQ ID no: 140) showed no expression at stage V3 but was high at stage V7 and then decreased at VT stage. Expression in roots under the control of EXP-Zm.UbqM1:1:11 (SEQ ID no: 150) was maintained at a similar level throughout development from stages V3 and V7 up to VT. Expression of GUS under the control of EXP-Cl.Ubq10 (SEQ ID NO: 168) was relatively stable from V4 to V7, but decreased by about half in VT as compared to V4 and V7.

[000115] GUS expression levels also showed significant differences in leaf tissue. EXP sequences EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), and EXP-Zm.UbqM1:1:11 (SEQ ID no: 138) showed the highest level of GUS expression observed at all three developmental stages (V3, V7 and VT). The EXP sequence EXP-Sb.Ubq4:1:2 (SEQ ID NO: 140) showed reduced expression at developmental stages V3 to VT. EXP sequences EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13) and EXP-Sb.Ubq7:1:2 (SEQ ID no: 150) showed higher levels of GUS expression at V3 and VT developmental stages, with more low level of expression in the middle of growth at the V7 stage of development. EXP sequence EXP-ARUdo.Ubq2:1:9 (SEQ ID no: 30), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41) and EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76) maintained GUS expression throughout all three steps, while EXP-ARUdo.Ubq2:1:8 (SEQ ID no: 27), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38) and EXP -BOUgr.Ubq2:1:15 (SEQ ID no: 55) showed a slight decrease in expression at the VT stage. Expression under the control of EXP-Cl.Ubq10 (SEQ ID NO: 168) was similar at V4 and VT stages, but decreased by about half at V7 stage compared to V4 and VT stages.

[000116] Similarly, with respect to the reproductive tissue (anther and silk), a different expression pattern was observed, unique to each EXP sequence. For example, high expression levels were observed in anther and silk for the EXP sequences: EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP- BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:15 (SEQ ID no: 55), EXP-SORnu.Ubq1:1:7 (SEQ ID no: 95), EXP -Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), EXP-Zm.UbqM1:1:11 (SEQ ID no: 138), and EXP-Sb.Ubq7:1:2 (SEQ ID NO: 150). EXP-controlled expression of sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-SORnu.Ubq1:1:6 (SEQ ID no: 92), EXP-Sv.Ubq1:1:11 (SEQ ID no: 114), EXP-Sv.Ubq1:1:12 (SEQ ID no: 120), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140), EXP-Sb.Ubq6:1:3 ( SEQ ID no: 148) and EXP-Cl.Ubq10 (SEQ ID no: 168) was high in anther but lower in silk for each EXP sequence, while EXP-BOUgr.Ubq1:1:6 was under control ( SEQ ID NO: 38) was higher in silk than in anther.

[000117] Expression in developing seeds (21 DPO ovary and endosperm) differed among EXP sequences. EXP sequences EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), and EXP-Zm.UbqM1:1:11 (SEQ ID no: 138) carried out a high expression of GUS in the tissues of the ovary and endosperm of developing seeds. Expression levels in the endosperm were about twice or more higher than in the ovary when GUS was under the control of the sequences EXP: EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1: 9 (SEQ ID NO: 18), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 27), EXP-BOUgr.Ubq2:1:14 (SEQ ID NO: 52), EXP-BOUgr.Ubq2:1 :15 (SEQ ID no: 55), EXP-SORnu.Ubq1:1:6 (SEQ ID no: 92), EXP-SORnu.Ubq1:1:7 (SEQ ID no: 95), EXP-Sv.Ubq1: 1:12 (SEQ ID no: 120), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Cl.Ubq10 (SEQ ID no: 168). GUS expression was three times higher in the ovary than in the endosperm under the control of EXP-Sb.Ubq7:1:2 (SEQ ID no: 150). GUS expression levels were relatively equivalent in ovary and endosperm when controlled by the EXP sequences: EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76) , EXP-SETit.Ubq1:1:10 (SEQ ID NO: 99) and EXP-Sb.Ubq6:1:3 (SEQ ID NO: 148).

[000118] Each EXP sequence showed the ability to control transgene expression in stably transformed maize plants. However, each EXP sequence had a unique expression pattern for each tissue, making it possible to select the EXP sequence that best expresses a particular transgene, depending on the tissue expression strategy required to achieve the desired results. This example shows that EXP sequences isolated from homologous genes do not necessarily behave equivalently in a transformed plant, and that expression can only be determined by empirical studies of the properties of each EXP sequence and cannot be predicted based on the homology of the gene from which the promoter is was received.

Example 6

Enhancers derived from regulatory elements.

[000119] Enhancers are derived from the promoter elements provided herein, such as those shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169. The enhancer element may contain one or more cis regulatory elements that, when operably linked at the 5' or 3' end to a promoter element, or operably linked at the 5' or 3' end to additional enhancer elements that are operably linked to the promoter, can increase or modulate expression the transgene or to allow the expression of the transgene in a particular cell type or plant organ, or at a particular time point in development or circadian rhythm. Enhancers are created by removing the TATA box or functionally similar elements and any underlying DNA sequence from the promoters, allowing transcription to be initiated from the promoters described herein as described above, including fragments thereof that have the TATA box or functionally similar elements removed. elements and DNA sequence below the TATA box.

[000120] Enhancer elements can be derived from the promoter elements described herein and cloned using methods known in the art such that they are operably linked via the 5' or 3' end to the promoter element or operably linked through the 5' or 3' end with additional enhancer elements that are operably linked to the promoter. Alternatively, enhancer elements are cloned using methods known in the art such that they are operably linked to one or more copies of an enhancer element that is operably linked via the 5' or 3' end to a promoter element or operably linked via 5 ' or 3' end with additional enhancer elements that are operably linked to the promoter. Enhancer elements can also be cloned so that they are operably linked at the 5' or 3' end to a promoter element derived from an organism of another species, or operably linked at the 5' or 3' end to additional enhancer elements derived from organisms of another species. of a genus or an organism of the same genus that is operably linked to a promoter derived from an organism of the same or another genus, resulting in a chimeric regulatory element. The GUS gene expression vector for plant transformation was generated using methods known in the art, similar to the genetic constructs described in the previous examples, in which the resulting plant expression vectors contain the right border region from A. tumefaciens , the first expression cassette for regulatory testing. or a chimeric regulatory element comprising a regulatory or chimeric regulatory element operably linked to an intron derived from the heat shock protein HSP70 from Z. mays (I-Zm.DnaK-1:1:1 SEQ ID no: 165) or any of the introns provided herein, or any other intron operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID no: 155) or no intron (GUS-1, SEQ ID no: 154), operably associated with the 3'-UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 158) or with the 3'-UTR of the rice lipid transfer protein gene (T-Os.LTP-1 :1:1, SEQ ID NO: 160); the second transgene selection cassette, used to select transformed plant cells, confers resistance to the herbicide glyphosate (under the control of the rice actin 1 promoter) or alternatively the antibiotic kanamycin (under the control of the rice actin 1 promoter) and the left boundary region from A. tumefaciens . The resulting plasmids are used to transform corn plants or other plants of this genus using the methods described previously, or other Agrobacterium -mediated transformation or microparticle bombardment methods known in the art. Alternatively, protoplast cells derived from corn or other plants of the genus are transformed using methods known in the art to perform transient expression analysis.

[000121] Expression of the GUS gene under the control of a regulatory element containing one or more enhancers is evaluated in stable transformation or transient expression plants to determine the effect of the enhancer element on transgene expression. Modifications of one or more enhancer elements or duplication of one or more enhancer elements are based on empirical studies and the resulting regulation of gene expression observed using the composition of each regulatory element. Changing the relative positions of one or more enhancers in the final regulatory or chimeric regulatory element can affect the transcriptional activity or specificity of the regulatory or chimeric regulatory element and is determined empirically to determine the best enhancers for the desired transgene expression profile within a corn plant or plant of another genus.

Example 7

Analysis of intron amplification of GUS activity using protoplasts of plant origin.

[000122] The intron is selected based on experimentation and comparison with the expression of a control intronless vector to empirically select the intron and configuration within the location of the DNA transfer elements (T-DNA) of the vector for optimal expression of the transgene. For example, when expressing a herbicide resistance gene such as CP4 that confers glyphosate tolerance, it is desirable to obtain expression of the transgene in reproductive tissues as well as vegetative tissues to prevent crop loss after herbicide application. The intron operably linked to the constitutive promoter will in this case be selected for its ability to enhance the expression of herbicide resistance conferred by the transgene, in particular in the reproductive cells and tissues of the transgenic plant and thus confer both vegetative and reproductive tolerance of the transgenic plant. when sprayed with herbicide. In most ubiquitin genes, the 5'-UTR consists of a leader that has an intron sequence inserted into it. Therefore, regulatory elements derived from such genes were analyzed using the entire 5'-UTR containing promoter, leader and intron. To achieve different expression profiles or modulate the expression level of a transgene, an intron from such a regulatory element can be removed or replaced with a heterologous intron.

[000123] The introns represented herein in SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171 were identified using genomic DNA contigs versus expressed clusters of tag sequences or cDNA contigs to identify exon and intron sequences within the genomic DNA. In addition, 5'-UTRs or leader sequences are also used to define intron/exon splicing junctions of one or more introns under conditions where the gene sequence encodes a leader sequence that is interrupted by one or more introns. The introns were cloned using methods known in the art into a plant transformation vector such that they are operably linked to the 3' regulatory element and leader fragment and operably linked to either the 5' second leader fragment or coding sequences, e.g. as shown in the expression cassettes shown in FIG. 9.

[000124] Thus, for example, the first possible expression cassette (expression cassette configuration 1 in Fig. 9) consists of a promoter or chimeric promoter element [A] operably linked to the 5' leader element [B] operably linked to the 5'- test intronic element [C] operably linked to a coding region [D] that is operably linked to the 3'-UTR element [E]. Alternatively, the second possible expression cassette (expression cassette configuration 2 in Figure 9) consists of a promoter or chimeric promoter element [F] operably linked to a 5' first leader element or fragment of the first leader element [G] operably linked to 5' test intronic element [H] operably linked to the 5' second leader element or second fragment of the first leader element [I] operably linked to the coding region [J] which is operably linked to the 3' UTR element [K] . Additionally, a third possible expression cassette (expression cassette configuration 3 in FIG. 9) consists of a promoter or chimeric promoter element [L] operably linked to the 5' leader element [M] operably linked to the 5' first fragment of the element of the coding sequence [N], operably linked to the 5'-intron element [O], operably linked to the 5'-second fragment of the coding sequence element [P], which is operably linked to the 3'-UTR element [Q]. The expression cassette 3 is designed to splice the intron in such a way as to obtain a full open reading frame with no frameshift between the first and second fragment of the coding sequence.

[000125] As previously discussed, it may be preferable to avoid using an AT nucleotide sequence or an A nucleotide sequence immediately before the 5' end of the splice site (GT) and a G nucleotide or nucleotide sequence TG, respectively, immediately after the 3' end of the splicing site (AG) , to eliminate potential unwanted start codons generated during the processing of the messenger RNA into the final transcript. The DNA sequence near the 5' or 3' end of the splicing sites of the intron can thus be modified.

[000126] Introns were tested for the effect of amplification by the ability to enhance expression in the analysis of transient expression or stable expression in the plant. To analyze the intron amplification of transient expression, a basic plant vector was constructed using methods known in the art. The intron was cloned into a basic plant vector that contains an expression cassette consisting of a constitutive promoter such as the cauliflower mosaic virus promoter P-CaMV.35S-enh-1:1:9 (SEQ ID NO: 166) operably linked to 5' -leader element L-CaMV.35S-1:1:15 (SEQ ID no: 167), functionally linked to the 5'-tested intron element ( for example, one of SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171) operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID NO: 155) or lacks an intron (GUS-1, SEQ ID no: 154) operably linked to the 3'UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 158). Protoplast cells derived from maize or other genus plant tissue were transformed with the plant base vector and luciferase gene control vectors as previously described in Example 2 above and analyzed for activity. To compare the relative ability of an intron to enhance expression, GUS values were expressed as the ratio of GUS activity to luciferase activity and compared with those obtained with a genetic construct containing a constitutive promoter operably linked to a known intron standard, such as an intron derived from the HSP70 protein. heat shock Zea mays , I-Zm.DnaK-1:1:1 (SEQ ID no: 165), as well as a genetic construct containing a constitutive promoter, but without an intron functionally associated with the promoter.

[000127] For analysis in stably transformed plants of introns shown in SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171, a GUS expression vector for plant transformation similar to the genetic constructs described in the previous examples was developed, in which the resulting plant expression vectors contain the right border region from A. tumefaciens, the first expression cassette for intron testing, consisting of a constitutive promoter , such as the cauliflower mosaic virus promoter P-CaMV.35S-enh-1:1:9 (SEQ ID NO: 166) operably linked to the 5' leader element of L-CaMV.35S-1:1:15 (SEQ ID no: 167) operably linked to the 5' test intron element provided herein operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID no: 155) or no intron (GUS -1, SEQ ID NO: 154) operably linked to the 3'UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID NO: 158); a second transgene selection cassette, used to select transformed plant cells, confers resistance to glyphosate (under the control of the rice actin 1 promoter) or alternatively the antibiotic kanamycin (under the control of the rice actin 1 promoter) and the left border region from A. tumefaciens . The resulting plasmids are used to transform maize plants or other plants of this genus using the methods described previously, or Agrobacterium -mediated methods known in the art. Single copy or low copy transformants are selected for comparison with single copy or low copy transformed plants transformed with a plant transformation vector identical to the test vector but without the test intron to determine if the test intron provides an intron-mediated enhancement effect.

[000128] Any of the introns shown in SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171 can be modified in several ways, such as deletion of fragments within the intron sequence, which can reduce expression, or duplication of fragments with an intron, which can increase expression. In addition, DNA sequences within an intron that may affect the specificity of expression, either in particular cell types or in tissues and organs, may be duplicated or altered or deleted in order to affect the expression and expression patterns of the transgene. In addition, the introns provided herein can be modified to remove any potential start codons (ATGs) that could cause unintended transcripts to be expressed from mis-spliced introns as different, extended, or shortened proteins. Once the intron has been empirically verified or altered based on the study, the intron is used to enhance the expression of the transgene in stably transformed plants, which may be monocots or dicots of any genus, provided that the intron enhances the transgene. An intron can also be used to enhance expression in other organisms, such as algae, fungi, or animal cells, provided that the intron provides increased or decreased, or specific expression of the transgene to which it is operatively linked.

[000129] It will be apparent to those skilled in the art from the illustrations and description of the principles of the invention that the form and details of this invention may be modified without departing from these principles. We claim all modifications that fall within the spirit and scope of the claims. All publications and published patent documents cited herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually identified as being incorporated by reference.

--->

SEQUENCE LIST

<110> Monsanto Technology LLC

<120> PLANT REGULATORY ELEMENTS AND THEIR USE

<130> MONS:323WO

<140> No information

<141> 2013-12-17

<150> 61/739.720

<151> 2012-12-19

<160> 171

<210> 1

<211> 3143

<212> DNA

<213> Agrostis nebulosa

<400> 1

ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg

60

agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt

120

acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca

180

ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga

240

cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg

300

tcacgtgtca tatttatgtt gagacgaaga agagaaaata aacaccatgc aaatttatgg

360

cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag

420

aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca

480

tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa

540

cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa

600

agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga

660

gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg

720

atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca

780

gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa

840

ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa

900

atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac

960

tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag

1020

ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata

1080

gaattctaag tatgaaatga ttttttttgtt atacaaaagg agtatatatc acaagattga

1140

tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg

1200

tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag

1260

gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt

1320

tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag

1380

ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt

1440

tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct

1500

aaaagtagag aaatcagaaa aagattcatg ttttggtagt tttgatttct tgcctccata

1560

ataattttgg tttaccattt tttgtttgat tttagtttta gaagcgttta tagcaggatt

1620

taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga

1680

caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa

1740

cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc

1800

ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt

1860

tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacgggg

1920

gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaaatagcca

1980

gccccgtccc cagattcttt cccaacctca tctttgttcg gagcacccac acaacccgat

2040

ccccaattcc ctcgtctctc ctcgcgagcc tcgtcgaccc ccccttcaag gtacggcgat

2100

cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg cgacccggtc

2160

catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg taaaatagat

2220

ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc ctttaggaca

2280

tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct aggcagtggg

2340

gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa ctgggaaacc

2400

tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga tgagatcgat

2460

ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt ccgtggtatg

2520

atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca tttaattgtc

2580

aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg ttgggctgta

2640

gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc tgtatgtgtc

2700

acatatatct tcatgattaa tatggttgga attatctctt catcttttag atatatatgg

2760

ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt catgcttaga

2820

tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata aacaaataag

2880

gataggtata tatgttgctg atggttttac tgatacttta ttagatagta cttctttgac

2940

atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt

3000

ttaattattt tgatatactt ggatgatgtc atgcagcagc tatgtgtgaa ttttcggccc

3060

tgtcttcata tgatgtttat ttgcttggga ctgtttcttt ggctgataac tcaccctgtt

3120

gtttggtgat ccttctgcag gtg

3143

<210> 2

<211> 2005

<212> DNA

<213> Agrostis nebulosa

<400> 2

ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg

60

agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt

120

acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca

180

ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga

240

cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg

300

tcacgtgtca tatttatgtt gagacgaaga agagaaaata aacaccatgc aaatttatgg

360

cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag

420

aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca

480

tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa

540

cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa

600

agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga

660

gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg

720

atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca

780

gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa

840

ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa

900

atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac

960

tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag

1020

ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata

1080

gaattctaag tatgaaatga ttttttttgtt atacaaaagg agtatatatc acaagattga

1140

tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg

1200

tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag

1260

gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt

1320

tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag

1380

ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt

1440

tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct

1500

aaaagtagag aaatcagaaa aagattcatg ttttggtagt tttgatttct tgcctccata

1560

ataattttgg tttaccattt tttgtttgat tttagtttta gaagcgttta tagcaggatt

1620

taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga

1680

caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa

1740

cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc

1800

ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt

1860

tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacgggg

1920

gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaaatagcca

1980

gccccgtccc cagattcttt cccaa

2005

<210> 3

<211> 85

<212> DNA

<213> Agrostis nebulosa

<400> 3

cctcatcttt gttcggagca cccacacaac ccgatcccca attccctcgt ctctcctcgc

60

gagcctcgtc gaccccccct tcaag

85

<210> 4

<211> 1053

<212> DNA

<213> Agrostis nebulosa

<400> 4

gtacggcgat cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg

60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg

120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc

180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct

240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa

300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga

360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt

420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca

480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg

540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc

600

tgtatgtgtc acatatatct tcatgattaa tatggttgga attatctctt catcttttag

660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt

720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata

780

aacaaataag gataggtata tatgttgctg atggttttac tgatacttta ttagatagta

840

cttctttgac atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa

900

agcttgcttt ttaattattt tgatatactt ggatgatgtc atgcagcagc tatgtgtgaa

960

ttttcggccc tgtcttcata tgatgtttat ttgcttggga ctgtttcttt ggctgataac

1020

tcaccctgtt gtttggtgat ccttctgcag gtg

1053

<210> 5

<211> 2137

<212> DNA

<213> Agrostis nebulosa

<400> 5

gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct

60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aaggagtata

120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta

180

gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta cattttgggta

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt

300

tgaaaagtga cggttttaag gatgggtaag ctgattagta tatgtaagtt tagctttttc

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga

420

gcccatataa gcgttcatga attgtacata tccttagatt ttttttttttg ggtaaagctc

480

gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat

540

ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg

600

tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg

660

tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag

720

cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct

780

ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt

840

ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc

900

accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc

960

gctataaata gccagccccg tccccagatt ctttcccaac ctcatctttg ttcggagcac

1020

ccacacaacc cgatccccaa ttccctcgtc tctcctcgcg agcctcgtcg accccccctt

1080

caaggtacgg cgatcgtcct ccctccctct ctctctctac cttctcttct ctagactaga

1140

tcggcgaccc ggtccatggt tagggcctgc tagttctgtt cctgtttttt ccatggctgc

1200

gaggtaaaat agatctgatg gcgttatgat ggttaactcg tcatactctt gcgatctatg

1260

gtccctttag gacatcgatt taatttcgga tggttcgaga tcggtgatcc atggttagta

1320

ccctaggcag tggggttaga tccgtgctgt tagggttcgt agatggattc tgattgctca

1380

gtaactggga aacctgggat ggttctagct gggaatcctg ggatggttct agctggttcg

1440

cagatgagat cgatttcatg gtctgctata tcttgtttcg ttgcctaggt tccgtttaat

1500

ctgtccgtgg tatgatgtta gcctttgata aggttcgatc gtgctagcta cgtcctgcgc

1560

agcatttaat tgtcaggtca taatttttag cattcctgtt tttgtttggt ttggttttgt

1620

ctggttgggc tgtagatagt ttcaatctac ctgtcggttt attttattaa atttggattg

1680

gatctgtatg tgtcacatat atcttcatga ttaatatggt tggaattatc tcttcatctt

1740

ttagatatat atggataggt atatatgttg ctgtgggttt tactggtact ttattagata

1800

tattcatgct tagatacatg aagcaacgtg ctgttacagt ttaataattc ttgtttatct

1860

aataaacaaa taaggatagg tatatatgtt gctgatggtt ttactgatac tttattagat

1920

agtacttctt tgacatgaag gaacatcctg cgacagctta ataattattc ttcatctaat

1980

aaaaagcttg ctttttaatt attttgatat acttggatga tgtcatgcag cagctatgtg

2040

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga

2100

taactcaccc tgttgtttgg tgatccttct gcaggtg

2137

<210> 6

<211> 999

<212> DNA

<213> Agrostis nebulosa

<400> 6

gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct

60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aaggagtata

120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta

180

gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta cattttgggta

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt

300

tgaaaagtga cggttttaag gatgggtaag ctgattagta tatgtaagtt tagctttttc

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga

420

gcccatataa gcgttcatga attgtacata tccttagatt ttttttttttg ggtaaagctc

480

gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat

540

ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg

600

tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg

660

tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag

720

cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct

780

ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt

840

ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc

900

accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc

960

gctataaata gccagccccg tccccagatt ctttcccaa

999

<210> 7

<211> 1900

<212> DNA

<213> Agrostis nebulosa

<400> 7

gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata

60

gtttgaaaag tgacggtttt aaggatggggt aagctgatta gtatatgtaa gtttagcttt

120

ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta

180

agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag

240

ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt

300

gatttcttgc ctccataata attttggttt accatttttt gtttgatttt agttttagaa

360

gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag

420

ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc

480

aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga

540

tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca

600

agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac

660

ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc

720

gccgctataa atagccagcc ccgtccccag attctttccc aacctcatct ttgttcggag

780

cacccacaca acccgatccc caattccctc gtctctcctc gcgagcctcg tcgacccccc

840

cttcaaggta cggcgatcgt cctccctccc tctctctctc taccttctct tctctagact

900

agatcggcga cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc

960

tgcgaggtaa aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct

1020

atggtccctt taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta

1080

gtaccctagg cagtggggtt agatccgtgc tgttagggtt cgtagatgga ttctgattgc

1140

tcagtaactg ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt

1200

tcgcagatga gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt

1260

aatctgtccg tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg

1320

cgcagcattt aattgtcagg tcataatttt tagcattcct gtttttgttt ggtttggttt

1380

tgtctggttg ggctgtagat agtttcaatc tacctgtcgg tttattttat taaatttgga

1440

ttggatctgt atgtgtcaca tatatcttca tgattaatat ggttggaatt atctcttcat

1500

cttttagata tatatggata ggtatatatg ttgctgtggg ttttactggt actttattag

1560

atatattcat gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta

1620

tctaataaac aaataaggat aggtatatat gttgctgatg gttttactga tactttatta

1680

gatagtactt ctttgacatg aaggaacatc ctgcgacagc ttaataatta ttcttcatct

1740

aataaaaagc ttgcttttta attattttga tatacttgga tgatgtcatg cagcagctat

1800

gtgtgaattt tcggccctgt cttcatatga tgtttatttg cttgggactg tttctttggc

1860

tgataactca ccctgttgtt tggtgatcct tctgcaggtg

1900

<210> 8

<211> 762

<212> DNA

<213> Agrostis nebulosa

<400> 8

gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata

60

gtttgaaaag tgacggtttt aaggatggggt aagctgatta gtatatgtaa gtttagcttt

120

ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta

180

agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag

240

ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt

300

gatttcttgc ctccataata attttggttt accatttttt gtttgatttt agttttagaa

360

gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag

420

ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc

480

aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga

540

tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca

600

agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac

660

ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc

720

gccgctataa atagccagcc ccgtccccag attctttccc aa

762

<210> 9

<211> 5068

<212> DNA

<213> Arundo donax

<400> 9

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga

60

ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag

120

gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga

180

tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc

240

agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa

300

aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg

360

tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg

420

aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc

480

ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga

540

tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata

600

tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt

660

gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata

720

attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt

780

gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg

840

tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata

900

catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc

960

accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact

1020

atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc

1080

acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt

1140

gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga

1200

agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt

1260

ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg

1320

gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc

1380

ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct

1440

caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc

1500

ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc

1560

cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt

1620

caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat

1680

ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta

1740

tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa

1800

gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga

1860

gctgacaaga acctccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg

1920

agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat

1980

catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc

2040

tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg

2100

aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt

2160

gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacgggg

2220

ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta

2280

actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca

2340

ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa

2400

ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt

2460

ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac

2520

aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca

2580

gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg

2640

accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga

2700

atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc

2760

actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg

2820

agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac

2880

ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac

2940

attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa

3000

aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa

3060

accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt

3120

atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca

3180

tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa

3240

ctggttcatg tttttattcag gttggtaaat gaattttgcc aattcaatgt agttagatat

3300

ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg

3360

tgacgtcttt cggaagatca agatcaatta tcaagtatca gcaacagcac ctgaaggttg

3420

gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca

3480

gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg

3540

gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt

3600

tgctgacggg acacgccact atggatgaaa ttggacaaaa tacgaatatt caaggatgaa

3660

agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaacttt gaggacgaag

3720

ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc

3780

cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc

3840

aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc

3900

tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc

3960

gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa

4020

ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa

4080

ataggcaccc cgtcctcgcc tcctctcccc acctcatctc ctcctttccc gtgaaccgtg

4140

aacacaaccc gacccagatc ccctcttgcg agcttcgtcg atccctcctc cgcgtcaagg

4200

tacggagctt ctcctcccc ttcttctcta gatcggcgtg ttatgttgtt tccgtggttg

4260

cttggttgga tgaatcgaat gattcttagg gcctaggagg ctggttagat ctgttgcgtt

4320

ctgtttcgta gatggatttt ggtgtaagat caggtcggtt ccgctgttta acttgtgatg

4380

ctagtgtgat ttttgggagg atttgagttg ttaatctgggg agttgttggg aggttctcgt

4440

aggcggattg tagatgaagt cgccgcacg atttgcgtgg cttgttggggt agctagggtt

4500

agatctgctc ggatttttca ttgttactta ttgagagata atgtagctaa cctttacttg

4560

ttcatctatg tatctcgtat tcgtattcat ctggttcgat ggtgctagat agatgcgcct

4620

gatttgtccg atcgaattgg gtagcatccg cggcttgttt ggtagtgttc tgattgattt

4680

gtcgctctag atctgagtgg aataatatta catctcaaca tgttactaga aacttggttt

4740

atagctccgg atttacatgt ttattcttat gtaaggtttt aaatgaaaga tttatgctac

4800

tgctgctcgt tgatccttta gcatccacct gaggaacatg catgcatctg ttacttcttt

4860

tgatatatgc ttagatagtt gttagtatat actgctgttg ttcgatgatc cttcaggatg

4920

aacatgcatg atcatgttac ttgtttttat atgcttctgc tgttcgttga ttctttagta

4980

ctacctacct gatcatcttg catgtttcct gcttgttaga gattaattga ttaggcttac

5040

cttgttgcct ggtgattctt ccttgcag

5068

<210> 10

<211> 4114

<212> DNA

<213> Arundo donax

<400> 10

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga

60

ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag

120

gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga

180

tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc

240

agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa

300

aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg

360

tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg

420

aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc

480

ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga

540

tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata

600

tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt

660

gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata

720

attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt

780

gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg

840

tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata

900

catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc

960

accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact

1020

atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc

1080

acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt

1140

gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga

1200

agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt

1260

ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg

1320

gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc

1380

ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct

1440

caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc

1500

ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc

1560

cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt

1620

caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat

1680

ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta

1740

tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa

1800

gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga

1860

gctgacaaga acctccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg

1920

agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat

1980

catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc

2040

tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg

2100

aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt

2160

gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacgggg

2220

ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta

2280

actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca

2340

ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa

2400

ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt

2460

ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac

2520

aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca

2580

gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg

2640

accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga

2700

atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc

2760

actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg

2820

agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac

2880

ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac

2940

attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa

3000

aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa

3060

accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt

3120

atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca

3180

tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa

3240

ctggttcatg tttttattcag gttggtaaat gaattttgcc aattcaatgt agttagatat

3300

ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg

3360

tgacgtcttt cggaagatca agatcaatta tcaagtatca gcaacagcac ctgaaggttg

3420

gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca

3480

gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg

3540

gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt

3600

tgctgacggg acacgccact atggatgaaa ttggacaaaa tacgaatatt caaggatgaa

3660

agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaacttt gaggacgaag

3720

ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc

3780

cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc

3840

aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc

3900

tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc

3960

gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa

4020

ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa

4080

ataggcaccc cgtcctcgcc tcctctcccc acct

4114

<210> 11

<211> 85

<212> DNA

<213> Arundo donax

<400> 11

catctcctcc tttcccgtga accgtgaaca caacccgacc cagatcccct cttgcgagct

60

tcgtcgatcc ctcctccgcg tcaag

85

<210> 12

<211> 869

<212> DNA

<213> Arundo donax

<400> 12

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt

60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt

120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat

180

gctagtgtga gttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt

300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt

360

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc

420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt

480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt

540

tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt

660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat

720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt

780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta

840

ccttgttgcc tggtgattct tccttgcag

869

<210> 13

<211> 2969

<212> DNA

<213> Arundo donax

<400> 13

gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga

60

cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct

120

agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac

180

tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc

240

ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg

300

actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt

360

ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa

420

caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg

480

gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac

540

cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat

600

gactatgatt tttcgtcgat tttataaaag tgaaaacaac cggttgatat ctatgcgcac

660

tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag

720

agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt

780

gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat

840

tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa

900

tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac

960

cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat

1020

ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata

1080

gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact

1140

ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt

1200

ccatgtcatt ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg

1260

acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga

1320

gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga

1380

gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga

1440

gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg

1500

ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag

1560

tggtcggttt gatagttcag ggatgaaatg tgtctttgggg caaactttga ggacgaagtt

1620

gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca

1680

ctccgagccg gcatgtgggg tcccctag tcagccactg tatggcgccg actagctcaa

1740

cggccacgaa cggccaacc accagcgcaa cctaaacggc gtaaacgttg acggcatctc

1800

tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt

1860

gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc

1920

gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat

1980

aggcaccccg tcctcgcctc ctctccccac ctcatctcct cctttcccgt gaaccgtgaa

2040

cacaacccga cccagatccc ctcttgcgag cttcgtcgat ccctcctccg cgtcaaggta

2100

cggagcttct cctccccctt cttctctaga tcggcgtgtt atgttgtttc cgtggttgct

2160

tggttggatg aatcgaatga ttcttagggc ctaggaggct ggttagatct gttgcgttct

2220

gtttcgtaga tggattttgg tgtaagatca ggtcggttcc gctgtttaac ttgtgatgct

2280

agtgtgattt ttgggaggat ttgagttgtt aatctgggag ttgttgggag gttctcgtag

2340

gcggattgta gatgaagtcg cccgcacgat ttgcgtggct tgttgggtag ctagggttag

2400

atctgctcgg atttttcatt gttacttatt gagagataat gtagctaacc tttacttgtt

2460

catctatgta tctcgtattc gtattcatct ggttcgatgg tgctagatag atgcgcctga

2520

tttgtccgat cgaattgggt agcatccgcg gcttgtttgg tagtgttctg attgatttgt

2580

cgctctagat ctgagtggaa taatattaca tctcaacatg ttactagaaa cttggtttat

2640

agctccggat ttacatgttt attcttatgt aaggttttaa atgaaagatt tatgctactg

2700

ctgctcgttg atcctttagc atccacctga ggaacatgca tgcatctgtt acttcttttg

2760

atatatgctt agatagttgt tagtatatac tgctgttgtt cgatgatcct tcaggatgaa

2820

catgcatgat catgttactt gttttatat gcttctgctg ttcgttgatt ctttagtact

2880

acctacctga tcatcttgca tgtttcctgc ttgttagaga ttaattgatt aggcttacct

2940

tgttgcctgg tgattcttcc ttgcaggtg

2969

<210> 14

<211> 2012

<212> DNA

<213> Arundo donax

<400> 14

gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga

60

cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct

120

agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac

180

tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc

240

ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg

300

actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt

360

ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa

420

caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg

480

gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac

540

cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat

600

gactatgatt tttcgtcgat tttataaaag tgaaaacaac cggttgatat ctatgcgcac

660

tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag

720

agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt

780

gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat

840

tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa

900

tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac

960

cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat

1020

ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata

1080

gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact

1140

ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt

1200

ccatgtcatt ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg

1260

acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga

1320

gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga

1380

gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga

1440

gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg

1500

ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag

1560

tggtcggttt gatagttcag ggatgaaatg tgtctttgggg caaactttga ggacgaagtt

1620

gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca

1680

ctccgagccg gcatgtgggg tcccctag tcagccactg tatggcgccg actagctcaa

1740

cggccacgaa cggccaacc accagcgcaa cctaaacggc gtaaacgttg acggcatctc

1800

tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt

1860

gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc

1920

gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat

1980

aggcaccccg tcctcgcctc ctctccccac ct

2012

<210> 15

<211> 872

<212> DNA

<213> Arundo donax

<400> 15

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt

60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt

120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat

180

gctagtgtga gttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt

300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt

360

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc

420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt

480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt

540

tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt

660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat

720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt

780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta

840

ccttgttgcc tggtgattct tccttgcagg tg

872

<210> 16

<211> 1954

<212> DNA

<213> Arundo donax

<400> 16

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga

60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat

120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt

180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga

240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga

300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta

360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg

420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaattttt

480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag

540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg

600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca

660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac

720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac

780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg

840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag

900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct

960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga

1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg

1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg

1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt

1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt

1260

gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt

1320

tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt

1440

tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat

1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat

1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact

1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta

1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac

1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc

1800

aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag

1920

gcttaccttg ttgcctggtg attcttcctt gcag

1954

<210> 17

<211> 1000

<212> DNA

<213> Arundo donax

<400> 17

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga

60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat

120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt

180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga

240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga

300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta

360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg

420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaattttt

480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag

540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg

600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca

660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac

720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac

780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg

840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag

900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct

960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct

1000

<210> 18

<211> 1957

<212> DNA

<213> Arundo donax

<400> 18

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga

60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat

120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt

180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga

240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga

300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta

360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg

420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaattttt

480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag

540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg

600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca

660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac

720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac

780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg

840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag

900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct

960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga

1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg

1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg

1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt

1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt

1260

gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt

1320

tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt

1440

tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat

1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat

1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact

1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta

1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac

1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc

1800

aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag

1920

gcttaccttg ttgcctggtg attcttcctt gcaggtg

1957

<210> 19

<211> 1957

<212> DNA

<213> Arundo donax

<400> 19

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga

60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat

120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt

180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga

240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga

300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta

360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg

420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaattttt

480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag

540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg

600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca

660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac

720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac

780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg

840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag

900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct

960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga

1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg

1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg

1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt

1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt

1260

gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt

1320

tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt

1440

tacttgttca tctatgtata tcgtattcgt attcatctgg ttcgatggtg ctagatagat

1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat

1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact

1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta

1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac

1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc

1800

aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag

1920

gcttaccttg ttgcctggtg attcttcctt gcaggtg

1957

<210> 20

<211> 872

<212> DNA

<213> Arundo donax

<400> 20

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt

60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt

120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat

180

gctagtgtga gttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt

300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt

360

gttcatctat gtatatcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc

420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt

480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt

540

tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt

660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat

720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt

780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta

840

ccttgttgcc tggtgattct tccttgcagg tg

872

<210> 21

<211> 1712

<212> DNA

<213> Arundo donax

<400> 21

gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt

60

ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac

120

agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac

180

ggagggaggg gcactatgaa tttttggtga cggagggagg ggcactatga atttttggct

240

ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga

300

aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa

360

gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct

420

ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct

480

caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat

540

ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct

600

cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga

660

accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata

720

aataggcacc ccgtcctcgc ctcctctccc cacctcatct cctcctttcc cgtgaaccgt

780

gaacacaacc cgacccagat cccctcttgc gagcttcgtc gatccctcct ccgcgtcaag

840

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt

900

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt

960

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat

1020

gctagtgtga gttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg

1080

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt

1140

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt

1200

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc

1260

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt

1320

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt

1380

tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta

1440

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt

1500

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat

1560

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt

1620

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta

1680

ccttgttgcc tggtgattct tccttgcagg tg

1712

<210> 22

<211> 755

<212> DNA

<213> Arundo donax

<400> 22

gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt

60

ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac

120

agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac

180

ggagggaggg gcactatgaa tttttggtga cggagggagg ggcactatga atttttggct

240

ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga

300

aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa

360

gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct

420

ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct

480

caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat

540

ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct

600

cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga

660

accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata

720

aataggcacc ccgtcctcgc ctcctctccc cacct

755

<210> 23

<211> 3276

<212> DNA

<213> Arundo donax

<400> 23

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc

60

ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt

120

gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta

180

tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa

240

tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca

300

cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac

360

acattggtca ttcgggagg agtaatcccc catagctagt tgttttgagt ttgactaccc

420

aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag

480

gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa

540

atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc

600

tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga

660

atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct

720

tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg

780

gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag

840

aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt

900

agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg

960

aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt

1020

ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga

1080

ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg

1140

atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg

1200

aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat

1260

tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt

1320

ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga

1380

acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa

1440

tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct

1500

tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tatttagaat

1560

acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc

1620

caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc

1680

agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc

1740

aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc

1800

tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc

1860

tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg

1920

agaggggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg

1980

gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgtctcatct

2040

cctcctgttc cgtgaaccgt gaacgcaacc cgacccccag atctctctcg cgagcatcgt

2100

cgatccctcc tccgcgtcaa ggtacggatc ttctccttcc tcccccttcc cctctgggtc

2160

ggcgtgtcgt gttgtttctc tagttgcttg gctggatgga tcgagtggtt cttagggctt

2220

agatggctgg ttagatctgt tgcgttctgt ttcgtagatg gatttttggt gtagatctgg

2280

taggttatgc tggttaactg gtgatgctcc tgcgattttt gggggatctg agttgttaat

2340

ctggtagttg tatggggttc tcgtagccgg attgtagatg aaatcgtccg cgcggtttgc

2400

gtggctcgtt ggttagctag ggttagatct gctcggattt ttcattgttc ctgattcaga

2460

gatgtagtta acctttactt gttcatcttt gtatctcgta ttcgtacctg catgtatgat

2520

ctgtttcgat ggtgctagat aggtgcgcct gatttgtccg atcgaatctg gtagcatgcg

2580

ctgtttgttt ggtagtgttc tgattgattt gtcgctctag atctgagtag aataggatta

2640

tttctcaaca tgatattaga agcttggttt atagctccgg attagcatgt atgttacatg

2700

tttattctta tgtaaggttt taaacggaag atatatgcta ctgctgctca ttgattcttt

2760

atcatccacc tgagtccatg catgcttctg ttacttcttt tgatatgtgc ttagatagct

2820

gttgatatgt actgctgctg ttagatgatc cttcaggatg aacatgcatg attctgttac

2880

ttgttttggt atgcttagat aaatcaagat acgcttctgc tgttcgttga ttctttagta

2940

ctacctacct gatcagctta gatagatcaa gatatgcttc tgctgttcgt tgattcttta

3000

gtaataccta cctgatcagc ttagatagat caagatacgc ttctgctgtt cgttgattct

3060

ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaaggtt gattgcttag

3120

gctcatcttt ttcttttcgt tgattctcta gtactaccta cctgataaac atgcatgttt

3180

tctgcttgtt aaagattgat tgcttagtct catctttttc tttctctttt gtctaccgcc

3240

aggcctaacc ttgttgctgg tgactctttc ttgcag

3276

<210> 24

<211> 2033

<212> DNA

<213> Arundo donax

<400> 24

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc

60

ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt

120

gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta

180

tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa

240

tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca

300

cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac

360

acattggtca ttcgggagg agtaatcccc catagctagt tgttttgagt ttgactaccc

420

aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag

480

gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa

540

atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc

600

tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga

660

atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct

720

tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg

780

gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag

840

aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt

900

agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg

960

aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt

1020

ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga

1080

ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg

1140

atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg

1200

aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat

1260

tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt

1320

ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga

1380

acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa

1440

tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct

1500

tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tatttagaat

1560

acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc

1620

caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc

1680

agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc

1740

aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc

1800

tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc

1860

tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg

1920

aggggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg

1980

gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgt

2033

<210> 25

<211> 88

<212> DNA

<213> Arundo donax

<400> 25

ctcatctcct cctgttccgt gaaccgtgaa cgcaacccga cccccagatc tctctcgcga

60

gcatcgtcga tccctcctcc gcgtcaag

88

<210> 26

<211> 1155

<212> DNA

<213> Arundo donax

<400> 26

gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct

60

agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt

120

gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg

180

tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct

240

cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg

300

gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg

360

ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata

420

ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct

480

gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa

540

gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt

600

aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc

660

atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt

720

tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata

780

aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag

840

atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct

900

tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa

960

acatgcatgt tttctgcttg ttaaaggttg attgcttagg ctcatctttt tcttttcgtt

1020

gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt

1080

gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt

1140

gactctttct tgcag

1155

<210> 27

<211> 3250

<212> DNA

<213> Arundo donax

<400> 27

gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa

60

taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca

120

aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata

180

ctacttctta aagggctatt ctttttctga atgtcgcagt tggttatttt aaccatatta

240

caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa

300

gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc

360

ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg

420

gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca

480

aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa

540

aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac

600

aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag

660

cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca

720

atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca

780

atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa

840

ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat

900

tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa

960

ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag

1020

gaagactgac tgaggaatgg attttggtgg accgaggaaa ttggtgctgg gttgcagagg

1080

catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag

1140

tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca

1200

aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac

1260

acctatatgc accattttcc tacatagatt tttaacttct tacttaacca tgttgactaa

1320

gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt

1380

atcttaatta gactttgatc atcaagtgga atcccattta tcttaataat cttggcaaca

1440

ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat

1500

atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt

1560

ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga

1620

ccccactcgt caggcactgt atgtaaacgt cagcaactcc gtggcaagta acgtcgagag

1680

gaggagcggg cctaacggcg ccgactagct caacggccac caaccagcca accaccagcg

1740

caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa

1800

gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc

1860

tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa

1920

ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc

1980

ccctcctcgc gtcctctccc ccgtctcatc tcctcctgtt ccgtgaaccg tgaacgcaac

2040

ccgaccccca gatctctctc gcgagcatcg tcgatccctc ctccgcgtca aggtacggat

2100

cttctccttc ctcccccttc ccctctgggt cggcgtgtcg tgttgtttct ctagttgctt

2160

ggctggatgg atcgagtggt tcttagggct tagatggctg gttagatctg ttgcgttctg

2220

tttcgtagat ggatttttgg tgtagatctg gtaggttatg ctggttaact ggtgatgctc

2280

ctgcgatttt tgggggatct gagttgttaa tctggtagtt gtatggggtt ctcgtagccg

2340

gattgtagat gaaatcgtcc gcgcggtttg cgtggctcgt tggttagcta gggttagatc

2400

tgctcggatt tttcattgtt cctgattcag agatgtagtt aacctttact tgttcatctt

2460

tgtatctcgt attcgtacct gcatgtatga tctgtttcga tggtgctaga taggtgcgcc

2520

tgatttgtcc gatcgaatct ggtagcatgc gctgtttgtt tggtagtgtt ctgattgatt

2580

tgtcgctcta gatctgagta gaataggatt atttctcaac atgatattag aagcttggtt

2640

tatagctccg gattagcatg tatgttacat gtttattctt atgtaaggtt ttaaacggaa

2700

gatatatgct actgctgctc attgattctt tatcatccac ctgagtccat gcatgcttct

2760

gttacttctt ttgatatgtg cttagatagc tgttgatatg tactgctgct gttagatgat

2820

ccttcaggat gaacatgcat gattctgtta cttgttttgg tatgcttaga taaatcaaga

2880

tacgcttctg ctgttcgttg attctttagt actacctacc tgatcagctt agatagatca

2940

agatatgctt ctgctgttcg ttgattcttt agtaatacct acctgatcag cttagataga

3000

tcaagatacg cttctgctgt tcgttgattc tctagtacta cctacctgat aaacatgcat

3060

gttttctgct tgttaaaggt tgattgctta ggctcatctt tttcttttcg ttgattctct

3120

agtactacct acctgataaa catgcatgtt ttctgcttgt taaagattga ttgcttagtc

3180

tcatcttttt ctttctcttt tgtctaccgc caggcctaac cttgttgctg gtgactcttt

3240

cttgcaggtg

3250

<210> 28

<211> 2004

<212> DNA

<213> Arundo donax

<400> 28

gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa

60

taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca

120

aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata

180

ctacttctta aagggctatt ctttttctga atgtcgcagt tggttatttt aaccatatta

240

caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa

300

gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc

360

ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg

420

gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca

480

aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa

540

aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac

600

aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag

660

cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca

720

atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca

780

atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa

840

ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat

900

tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa

960

ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag

1020

gaagactgac tgaggaatgg attttggtgg accgaggaaa ttggtgctgg gttgcagagg

1080

catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag

1140

tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca

1200

aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac

1260

acctatatgc accattttcc tacatagatt tttaacttct tacttaacca tgttgactaa

1320

gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt

1380

atcttaatta gactttgatc atcaagtgga atcccattta tcttaataat cttggcaaca

1440

ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat

1500

atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt

1560

ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga

1620

ccccactcgt caggcactgt atgtaaacgt cagcaactcc gtggcaagta acgtcgagag

1680

gaggagcggg cctaacggcg ccgactagct caacggccac caaccagcca accaccagcg

1740

caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa

1800

gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc

1860

tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa

1920

ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc

1980

ccctcctcgc gtcctctccc ccgt

2004

<210> 29

<211> 1158

<212> DNA

<213> Arundo donax

<400> 29

gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct

60

agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt

120

gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg

180

tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct

240

cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg

300

gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg

360

ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata

420

ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct

480

gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa

540

gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt

600

aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc

660

atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt

720

tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata

780

aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag

840

atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct

900

tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa

960

acatgcatgt tttctgcttg ttaaaggttg attgcttagg ctcatctttt tcttttcgtt

1020

gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt

1080

gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt

1140

gactctttct tgcaggtg

1158

<210> 30

<211> 2247

<212> DNA

<213> Arundo donax

<400> 30

tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg

60

gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga

120

gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat

180

aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg

240

aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac

300

ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa

360

tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct

420

taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca

480

tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta

540

cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct

600

aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg

660

gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa

720

ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc

780

gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg

840

gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagggg

900

ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt

960

ctccataaat aggcgcccc tcctcgcgtc ctctcccccg tctcatctcc tcctgttccg

1020

tgaaccgtga acgcaacccg accccgat ctctctcgcg agcatcgtcg atccctcctc

1080

cgcgtcaagg tacggatctt ctccttcctc ccccttcccc tctgggtcgg cgtgtcgtgt

1140

tgtttctcta gttgcttggc tggatggatc gagtggttct tagggcttag atggctggtt

1200

agatctgttg cgttctgttt cgtagatgga tttttggtgt agatctggta ggttatgctg

1260

gttaactggt gatgctcctg cgatttttgg gggatctgag ttgttaatct ggtagttgta

1320

tggggttctc gtagccggat tgtagatgaa atcgtccgcg cggtttgcgt ggctcgttgg

1380

ttagctaggg ttagatctgc tcggattttt cattgttcct gattcagaga tgtagttaac

1440

ctttacttgt tcatctttgt atctcgtatt cgtacctgca tgtatgatct gtttcgatgg

1500

tgctagatag gtgcgcctga tttgtccgat cgaatctggt agcatgcgct gtttgtttgg

1560

tagtgttctg attgatttgt cgctctagat ctgagtagaa taggattatt tctcaacatg

1620

atattagaag cttggtttat agctccggat tagcatgtat gttacatgtt tattcttatg

1680

taaggtttta aacggaagat atatgctact gctgctcatt gattctttat catccacctg

1740

agtccatgca tgcttctgtt acttcttttg atatgtgctt agatagctgt tgatatgtac

1800

tgctgctgtt agatgatcct tcaggatgaa catgcatgat tctgttactt gttttggtat

1860

gcttagataa atcaagatac gcttctgctg ttcgttgatt ctttagtact acctacctga

1920

tcagcttaga tagatcaaga tatgcttctg ctgttcgttg attctttagt aatacctacc

1980

tgatcagctt agatagatca agatacgctt ctgctgttcg ttgattctct agtactacct

2040

acctgataaa catgcatgtt ttctgcttgt taaaggttga ttgcttaggc tcatcttttt

2100

cttttcgttg attctctagt actacctacc tgataaacat gcatgttttc tgcttgttaa

2160

agattgattg cttagtctca tctttttctt tctcttttgt ctaccgccag gcctaacctt

2220

gttgctggtg actctttctt gcaggtg

2247

<210> 31

<211> 1001

<212> DNA

<213> Arundo donax

<400> 31

tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg

60

gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga

120

gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat

180

aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg

240

aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac

300

ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa

360

tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct

420

taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca

480

tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta

540

cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct

600

aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg

660

gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa

720

ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc

780

gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg

840

gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagggg

900

ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt

960

ctccataaat aggcgcccc tcctcgcgtc ctctcccccg t

1001

<210> 32

<211> 1942

<212> DNA

<213> Arundo donax

<400> 32

catgttgact aagagcaagt ggaagcact ctcatttcat agaacaagtg atgaatgcca

60

acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata

120

atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt

180

aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa

240

atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc

300

ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag

360

taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc

420

caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc

480

ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg

540

ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac

600

ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca

660

taaataggcg ccccctcctc gcgtcctctc ccccgtctca tctcctcctg ttccgtgaac

720

cgtgaacgca acccgacccc cagatctctc tcgcgagcat cgtcgatccc tcctccgcgt

780

caaggtacgg atcttctcct tcctccccct tcccctctgg gtcggcgtgt cgtgttgttt

840

ctctagttgc ttggctggat ggatcgagtg gttcttaggg cttagatggc tggttagatc

900

tgttgcgttc tgtttcgtag atggattttt ggtgtagatc tggtaggtta tgctggttaa

960

ctggtgatgc tcctgcgatt tttgggggat ctgagttgtt aatctggtag ttgtatgggg

1020

ttctcgtagc cggattgtag atgaaatcgt ccgcgcggtt tgcgtggctc gttggttagc

1080

tagggttaga tctgctcgga tttttcattg ttcctgattc agagatgtag ttaaccttta

1140

cttgttcatc tttgtatctc gtattcgtac ctgcatgtat gatctgtttc gatggtgcta

1200

gataggtgcg cctgatttgt ccgatcgaat ctggtagcat gcgctgtttg tttggtagtg

1260

ttctgattga tttgtcgctc tagatctgag tagaatagga ttatttctca acatgatatt

1320

agaagcttgg tttatagctc cggattagca tgtatgttac atgtttattc ttatgtaagg

1380

ttttaaacgg aagatatatg ctactgctgc tcattgattc tttatcatcc acctgagtcc

1440

atgcatgctt ctgttacttc ttttgatatg tgcttagata gctgttgata tgtactgctg

1500

ctgttagatg atccttcagg atgaacatgc atgattctgt tacttgtttt ggtatgctta

1560

gataaatcaa gatacgcttc tgctgttcgt tgattcttta gtactaccta cctgatcagc

1620

ttagatagat caagatatgc ttctgctgtt cgttgattct ttagtaatac ctacctgatc

1680

agcttagata gatcaagata cgcttctgct gttcgttgat tctctagtac tacctacctg

1740

ataaacatgc atgttttctg cttgttaaag gttgattgct taggctcatc tttttctttt

1800

cgttgattct ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaagatt

1860

gattgcttag tctcatcttt ttctttctct tttgtctacc gccaggccta accttgttgc

1920

tggtgactct ttcttgcagg tg

1942

<210> 33

<211> 696

<212> DNA

<213> Arundo donax

<400> 33

catgttgact aagagcaagt ggaagcact ctcatttcat agaacaagtg atgaatgcca

60

acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata

120

atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt

180

aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa

240

atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc

300

ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag

360

taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc

420

caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc

480

ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg

540

ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac

600

ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca

660

taaataggcg ccccctcctc gcgtcctctc ccccgt

696

<210> 34

<211> 3511

<212> DNA

<213> Bouteloua gracilis

<400> 34

gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg

60

attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag

120

ccactagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg

180

caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct

240

gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt

300

gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg

360

cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg

420

gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca

480

cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta

540

tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag

600

agcaagtgta taatgaaata atgtaagcga gctatatgaa ttgtcacgtg tcatatttat

660

gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca

720

gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt

780

ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta

840

tatgagtgaa ttgataaatt tattctagggt gacatggcct taacgatgaa cagtacgtgg

900

ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga

960

ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt

1020

ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca

1080

ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta

1140

taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa

1200

tgctcaagat tcattcaaca attgaaacgt ctcacatgat taaaccatgt ataaggatgc

1260

taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat

1320

caaataataa ttttaaaaca attttataag agaagctccg gagataaaag ggcatctaat

1380

ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa

1440

tgattttttt gttatacgaa aggagtatat atcacaagat tgatgtcagt tatgcttagg

1500

gcacgtacac gacgctggtg ctttaggtag acgttaatcg ttgtttctgc attttatttt

1560

attttgttgc cacggtgtac atttggggtag acgtttgtca caggcattgc cactcaaaca

1620

agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc

1680

tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac

1740

aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat

1800

ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc

1860

agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac

1920

cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta

1980

ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca

2040

ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga

2100

cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca

2160

cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg

2220

ttccgttccg ccctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag

2280

cacggggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa

2340

tagccagccc cgtccccaga ttctttccca acctcatctt tgttcggagc acgcacacaa

2400

cccgatcccc aattccctcg tctctcctcg cgagcctcgt cgaccccccc cttcaaggta

2460

cggcgatcat cctccctccc tccctctctc taccttctct tctctagact agatcggcga

2520

cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc tgcgaggtaa

2580

aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct atggtccctt

2640

taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta gtaccctagg

2700

cagtggggtt agatccgtgc tgttagggtt cgtagatgga ttctgattgc tcagtaactg

2760

ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt tcgcagatga

2820

gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt aatctgtccg

2880

tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg cgcagcattt

2940

aattgtcagg tcataatttt tagcattcct gtttttgttt ggtttggttt tgtctggttg

3000

ggctgtagat agtttcaatc tacctgtcgg tttattttat taaatttgga ttggatctgt

3060

atgtgtcaca tatatcttca tgattaagat ggttggaatt atctcttcat cttttagata

3120

tatatggata ggtatatatg ttgctgtggg ttttactggt actttattag atatattcat

3180

gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta tctaataaac

3240

aaataaggat aggtatatgt tgctgatggt tttactgata ctttattaga tagtactttg

3300

acatgaagga acatcctgcg acagcttaat aattattctt catctaataa aaagcttgct

3360

ttttaattat tttaattatt ttgatatact tggatgatgt catgcagcag ctatgtgtga

3420

attttcggcc ctgtcttcat atgatgttta tttgcttggg actgtttctt tggctgataa

3480

cttaccctgt tgtttggtga tccttctgca g

3511

<210> 35

<211> 2371

<212> DNA

<213> Bouteloua gracilis

<400> 35

gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg

60

attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag

120

ccactagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg

180

caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct

240

gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt

300

gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg

360

cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg

420

gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca

480

cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta

540

tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag

600

agcaagtgta taatgaaata atgtaagcga gctatatgaa ttgtcacgtg tcatatttat

660

gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca

720

gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt

780

ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta

840

tatgagtgaa ttgataaatt tattctagggt gacatggcct taacgatgaa cagtacgtgg

900

ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga

960

ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt

1020

ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca

1080

ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta

1140

taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa

1200

tgctcaagat tcattcaaca attgaaacgt ctcacatgat taaaccatgt ataaggatgc

1260

taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat

1320

caaataataa ttttaaaaca attttataag agaagctccg gagataaaag ggcatctaat

1380

ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa

1440

tgattttttt gttatacgaa aggagtatat atcacaagat tgatgtcagt tatgcttagg

1500

gcacgtacac gacgctggtg ctttaggtag acgttaatcg ttgtttctgc attttatttt

1560

attttgttgc cacggtgtac atttggggtag acgtttgtca caggcattgc cactcaaaca

1620

agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc

1680

tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac

1740

aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat

1800

ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc

1860

agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac

1920

cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta

1980

ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca

2040

ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga

2100

cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca

2160

cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg

2220

ttccgttccg ccctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag

2280

cacggggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa

2340

tagccagccc cgtccccaga ttctttccca a

2371

<210> 36

<211> 86

<212> DNA

<213> Bouteloua gracilis

<400> 36

cctcatcttt gttcggagca cgcacacaac ccgatcccca attccctcgt ctctcctcgc

60

gagcctcgtc gacccccccc ttcaag

86

<210> 37

<211> 1054

<212> DNA

<213> Bouteloua gracilis

<400> 37

gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg

60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg

120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc

180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct

240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa

300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga

360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt

420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca

480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg

540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc

600

tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag

660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt

720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata

780

aacaaataag gataggtata tgttgctgat ggttttactg atacttttatt agatagtact

840

ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt

900

gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg

960

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga

1020

taacttaccc tgttgtttgg tgatccttct gcag

1054

<210> 38

<211> 3142

<212> DNA

<213> Bouteloua gracilis

<400> 38

gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc

60

ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg

120

tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac

180

ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata

240

atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag

300

aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag

360

gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag

420

gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt

480

gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag

540

aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg

600

caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca

660

aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc

720

gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac

780

ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc

840

attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct

900

tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt

960

ttaaaacaat tttataagag aagctccggga gataaaaggg catctaatct atgttagaag

1020

agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt

1080

tatacgaaag gagtatatat cacaagattg atgtcagtta tgcttagggc acgtacacga

1140

cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca

1200

cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc

1260

ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat

1320

gtaagtttag ctttttccat tgtaggttaa gccttaaggc tcttacacaa ttgtttcatt

1380

attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt

1440

ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc

1500

atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt

1560

gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca

1620

agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa

1680

ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc

1740

ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca

1800

cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc

1860

tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt

1920

ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg

1980

tccccagatt ctttcccaac ctcatctttg ttcggagcac gcacacaacc cgatccccaa

2040

ttccctcgtc tctcctcgcg agcctcgtcg acccccccct tcaaggtacg gcgatcatcc

2100

tccctccctc cctctctcta ccttctcttc tctagactag atcggcgacc cggtccatgg

2160

ttagggcctg ctagttctgt tcctgttttt tccatggctg cgaggtaaaa tagatctgat

2220

ggcgttatga tggttaactc gtcatactct tgcgatctat ggtcccttta ggacatcgat

2280

ttaatttcgg atggttcgag atcggtgatc catggttagt accctaggca gtggggttag

2340

atccgtgctg ttagggttcg tagatggatt ctgattgctc agtaactggg aaacctggga

2400

tggttctagc tgggaatcct gggatggttc tagctggttc gcagatgaga tcgatttcat

2460

ggtctgctat atcttgtttc gttgcctagg ttccgtttaa tctgtccgtg gtatgatgtt

2520

agcctttgat aaggttcgat cgtgctagct acgtcctgcg cagcatttaa ttgtcaggtc

2580

ataattttta gcattcctgt ttttgtttgg tttggttttg tctggttgggg ctgtagatag

2640

tttcaatcta cctgtcggtt tattttatta aatttggatt ggatctgtat gtgtcacata

2700

tatcttcatg attaagatgg ttggaattat ctcttcatct tttagatata tatggatagg

2760

tatatatgtt gctgtgggtt ttactggtac tttattagat atattcatgc ttagatacat

2820

gaagcaacgt gctgttacag tttaataatt cttgtttatc taataaacaa ataaggatag

2880

gtatatgttg ctgatggttt tactgatact ttattagata gtactttgac atgaaggaac

2940

atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt ttaattattt

3000

taattatttt gatatacttg gatgatgtca tgcagcagct atgtgtgaat tttcggccct

3060

gtcttcatat gatgtttatt tgcttgggac tgtttctttg gctgataact taccctgttg

3120

tttggtgatc cttctgcagg tg

3142

<210> 39

<211> 1999

<212> DNA

<213> Bouteloua gracilis

<400> 39

gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc

60

ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg

120

tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac

180

ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata

240

atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag

300

aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag

360

gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag

420

gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt

480

gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag

540

aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg

600

caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca

660

aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc

720

gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac

780

ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc

840

attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct

900

tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt

960

ttaaaacaat tttataagag aagctccggga gataaaaggg catctaatct atgttagaag

1020

agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt

1080

tatacgaaag gagtatatat cacaagattg atgtcagtta tgcttagggc acgtacacga

1140

cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca

1200

cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc

1260

ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat

1320

gtaagtttag ctttttccat tgtaggttaa gccttaaggc tcttacacaa ttgtttcatt

1380

attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt

1440

ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc

1500

atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt

1560

gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca

1620

agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa

1680

ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc

1740

ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca

1800

cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc

1860

tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt

1920

ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg

1980

tccccagatt ctttcccaa

1999

<210> 40

<211> 1057

<212> DNA

<213> Bouteloua gracilis

<400> 40

gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg

60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg

120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc

180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct

240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa

300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga

360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt

420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca

480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg

540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc

600

tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag

660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt

720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata

780

aacaaataag gataggtata tgttgctgat ggttttactg atacttttatt agatagtact

840

ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt

900

gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg

960

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga

1020

taacttaccc tgttgtttgg tgatccttct gcaggtg

1057

<210> 41

<211> 2165

<212> DNA

<213> Bouteloua gracilis

<400> 41

gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct

60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata

120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta

180

gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta cattttgggta

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt

300

tgaaaagtga cggttttaat gatgggtaag ctgattagta tatgtaagtt tagctttttc

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga

420

gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc

480

tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg

540

atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag

600

cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc

660

cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca

720

agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat

780

ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt

840

ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc

900

agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct

960

tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc

1020

aacctcatct ttgttcggag cacgcacaca acccgatccc caattccctc gtctctcctc

1080

gcgagcctcg tcgacccccc ccttcaaggt acggcgatca tcctccctcc ctccctctct

1140

ctaccttctc ttctctagac tagatcggcg acccggtcca tggttagggc ctgctagttc

1200

tgttcctgtt ttttccatgg ctgcgaggta aaatagatct gatggcgtta tgatggttaa

1260

ctcgtcatac tcttgcgatc tatggtccct ttaggacatc gatttaattt cggatggttc

1320

gagatcggtg atccatggtt agtaccctag gcagtggggt tagatccgtg ctgttagggt

1380

tcgtagatgg attctgattg ctcagtaact gggaaacctg ggatggttct agctgggaat

1440

cctgggatgg ttctagctgg ttcgcagatg agatcgattt catggtctgc tatatcttgt

1500

ttcgttgcct aggttccgtt taatctgtcc gtggtatgat gttagccttt gataaggttc

1560

gatcgtgcta gctacgtcct gcgcagcatt taattgtcag gtcataattt ttagcattcc

1620

tgtttttgtt tggtttggtt ttgtctggtt gggctgtaga tagtttcaat ctacctgtcg

1680

gtttatttta ttaaatttgg attggatctg tatgtgtcac atatatcttc atgattaaga

1740

tggttggaat tatctcttca tcttttagat atatatggat aggtatatat gttgctgtgg

1800

gttttactgg tactttatta gatatattca tgcttagata catgaagcaa cgtgctgtta

1860

cagtttaata attcttgttt atctaataaa caaataagga taggtatatg ttgctgatgg

1920

ttttactgat actttattag atagtacttt gacatgaagg aacatcctgc gacagcttaa

1980

taattattct tcatctaata aaaagcttgc tttttaatta ttttaattat tttgatatac

2040

ttggatgatg tcatgcagca gctatgtgtg aattttcggc cctgtcttca tatgatgttt

2100

atttgcttgg gactgtttct ttggctgata acttaccctg ttgtttggtg atccttctgc

2160

aggtg

2165

<210> 42

<211> 1022

<212> DNA

<213> Bouteloua gracilis

<400> 42

gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct

60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata

120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta

180

gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta cattttgggta

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt

300

tgaaaagtga cggttttaat gatgggtaag ctgattagta tatgtaagtt tagctttttc

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga

420

gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc

480

tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg

540

atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag

600

cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc

660

cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca

720

agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat

780

ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt

840

ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc

900

agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct

960

tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc

1020

aa

1022

<210> 43

<211> 1903

<212> DNA

<213> Bouteloua gracilis

<400> 43

actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga

60

tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg

120

ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat

180

tgtacatatc cttagatgtt ttttttttttg ggtaaagctc gagcttctct atctaaaagt

240

agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt

300

ttggtttacc attttttgtt tgattttagt

360

ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc

420

caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg

480

agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt

540

ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt

600

tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg

660

caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc

720

cgctataaat agccagcccc gtccccagat tctttcccaa cctcatcttt gttcggagca

780

cgcacacaac ccgatcccca attccctcgt ctctcctcgc gagcctcgtc gacccccccc

840

ttcaaggtac ggcgatcatc ctccctccct ccctctctct accttctctt ctctagacta

900

gatcggcgac ccggtccatg gttagggcct gctagttctg ttcctgtttt ttccatggct

960

gcgaggtaaa atagatctga tggcgttatg atggttaact cgtcatactc ttgcgatcta

1020

tggtcccttt aggacatcga tttaatttcg gatggttcga gatcggtgat ccatggttag

1080

taccctaggc agtggggtta gatccgtgct gttagggttc gtagatggat tctgattgct

1140

cagtaactgg gaaacctgggg atggttctag ctgggaatcc tgggatggtt ctagctggtt

1200

cgcagatgag atcgatttca tggtctgcta tatcttgttt cgttgcctag gttccgttta

1260

atctgtccgt ggtatgatgt tagcctttga taaggttcga tcgtgctagc tacgtcctgc

1320

gcagcattta attgtcaggt cataattttt agcattcctg ttttgtttg gtttggtttt

1380

gtctggttgg gctgtagata gtttcaatct acctgtcggt ttattttatt aaatttggat

1440

tggatctgta tgtgtcacat atatcttcat gattaagatg gttggaatta tctcttcatc

1500

ttttagatat atatggatag gtatatatgt tgctgtgggt tttactggta ctttattaga

1560

tatattcatg cttagataca tgaagcaacg tgctgttaca gtttaataat tcttgtttat

1620

ctaataaaca aataaggata ggtatatgtt gctgatggtt ttactgatac tttattagat

1680

agtactttga catgaaggaa catcctgcga cagcttaata attattcttc atctaataaa

1740

aagcttgctt tttaattatt ttaattattt tgatatactt ggatgatgtc atgcagcagc

1800

tatgtgtgaa ttttcggccc tgtcttcata tgatgtttat ttgcttggga ctgtttcttt

1860

ggctgataac ttaccctgtt gtttggtgat ccttctgcag gtg

1903

<210> 44

<211> 760

<212> DNA

<213> Bouteloua gracilis

<400> 44

actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga

60

tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg

120

ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat

180

tgtacatatc cttagatgtt ttttttttttg ggtaaagctc gagcttctct atctaaaagt

240

agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt

300

ttggtttacc attttttgtt tgattttagt

360

ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc

420

caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg

480

agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt

540

ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt

600

tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg

660

caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc

720

cgctataaat agccagcccc gtccccagat tctttcccaa

760

<210> 45

<211> 3234

<212> DNA

<213> Bouteloua gracilis

<400> 45

ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag

60

ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag

120

tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg

180

ttaagatgaa gaggagagaa taaacggtac gtaaatttat agcgagtgat agacgggcac

240

gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta

300

taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt

360

tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa

420

atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc

480

catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca

540

agtttttttt ttcttgcaaa aattgtttct tttttttttaa aaaagtataa tttggatcgt

600

gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt

660

gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat

720

aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat

780

taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat

840

tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg

900

tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc

960

taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc

1020

accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga

1080

aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt

1140

taccatttttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt

1200

ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac

1260

acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag

1320

attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc

1380

ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa

1440

tatttgaacc cgagtgggta gattccaccg caaaggacct aaccagatca tttcgcaaac

1500

aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa

1560

agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt

1620

tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc

1680

tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt

1740

cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt

1800

ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg

1860

agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc

1920

cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg ccctcctctca

1980

cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc

2040

cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa

2100

cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct

2160

cgtcgcgagc ctcgtcgatc cccgcttcaa ggtacagcga tcgatcgatc atcctcgctc

2220

tctctacctt ctctctctta gggcgtgctg gttctgttcc tgtttttcca tggctgcgag

2280

gtacaataga ttggcgattc atggttaggg cctgctagtt ctgttcctgt tttttttttt

2340

tccatggctg cgaggcacaa tagatctgat ggcgttatga tggttaactt gtcatactct

2400

tgcgatctat ggtcccttta ggagtttagg acatcgattt aatttcggat agttcgagat

2460

ctgtgatcca tggttagtac cctaggcagt ggggttagat ccgtgctgtt atggttcgta

2520

gatggattct gattgctcag taactgggaa tcctgggatg gttctagctg gttcgcagat

2580

aagatcgatt tcatgatatg ctatatcttg tttggttgcc gtggttccgt taaatctgtc

2640

tgttatgatc ttagtctttg ataaggttcg gtcgtgctag ctacgtcctg tgcagcactt

2700

aattgtcagg tcataatttt tagcatgcct tttttttatt ggtttggttt tgtctgactg

2760

ggctgtagat agtttcaatc tttgtctgac tgggctgtag atagtttcaa tcttcctgtc

2820

tgtttatttt attaaatttg gatctgtatg tgtgtcatat atcttcatct tttagatata

2880

tcgataggta tatatgttgc tgtcgttttt tactgttcct ttatgagata tattcatgct

2940

tagatacatg aaaaacgtg ctgttacagt ttaatagttc ttgtttatct aataaacaaa

3000

taaggatagg tgctgcagtt agttttactg gtactttttt tgacatgaac ctacggctta

3060

ataattagtc ttcatcaaat aaaaagcata tttttaatt atttcgatat acttgaatga

3120

tgtcatatgc agcatctgtg tgaatttttg gccctgtctt catatgatgt ttatttgctt

3180

gggactgttt ctttggctga taactcaccc tgttgtttgg tgatccttct gcag

3234

<210> 46

<211> 2100

<212> DNA

<213> Bouteloua gracilis

<400> 46

ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag

60

ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag

120

tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg

180

ttaagatgaa gaggagagaa taaacggtac gtaaatttat agcgagtgat agacgggcac

240

gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta

300

taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt

360

tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa

420

atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc

480

catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca

540

agtttttttt ttcttgcaaa aattgtttct tttttttttaa aaaagtataa tttggatcgt

600

gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt

660

gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat

720

aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat

780

taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat

840

tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg

900

tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc

960

taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc

1020

accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga

1080

aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt

1140

taccatttttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt

1200

ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac

1260

acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag

1320

attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc

1380

ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa

1440

tatttgaacc cgagtgggta gattccaccg caaaggacct aaccagatca tttcgcaaac

1500

aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa

1560

agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt

1620

tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc

1680

tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt

1740

cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt

1800

ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg

1860

agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc

1920

cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg ccctcctctca

1980

cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc

2040

cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa

2100

<210> 47

<211> 91

<212> DNA

<213> Bouteloua gracilis

<400> 47

cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct

60

cgtcgcgagc ctcgtcgatc cccgcttcaa g

91

<210> 48

<211> 1043

<212> DNA

<213> Bouteloua gracilis

<400> 48

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg

180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga

240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg

300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat

360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt

420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg

480

tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt

540

tttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact

600

gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt

660

gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt

720

actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt

780

taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg

840

tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat

900

tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg

960

ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct

1020

gttgtttggt gatccttctg cag

1043

<210> 49

<211> 3176

<212> DNA

<213> Bouteloua gracilis

<400> 49

aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc

60

aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt

120

atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacgggg

180

cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta

240

ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa

300

cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat

360

aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca

420

agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc

480

tcaagttttt ttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat

540

cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg

600

cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt

660

tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat

720

gattaaatca tataaagttt ctaagtcttg tttgacaaga ttttttttaga ttttcatcta

780

aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa

840

aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa

900

ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat

960

gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag

1020

agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg

1080

gtttaccatt ttttgtttga ttttagtttt agagaagcgt ttataacagg acctaaaatc

1140

tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg

1200

aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta

1260

gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa

1320

agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt

1380

gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca

1440

aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt

1500

gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat

1560

ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc

1620

ctctatttta ttccactaga attgattata aatttagcct cttaaatttt ataagttggg

1680

agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc

1740

cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa cggagaacc accgccagcg

1800

ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc

1860

tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc

1920

tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc

1980

ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc

2040

caacctcatc ttctcgtgtt gttcggccca acccgatcga tccccaattc cctcgtcgtc

2100

tctcgtcgcg agcctcgtcg atccccgctt caaggtacag cgatcgatcg atcatcctcg

2160

ctctctctac cttctctctc ttagggcgtg ctggttctgt tcctgttttt ccatggctgc

2220

gaggtacaat agattggcga ttcatggtta gggcctgcta gttctgttcc tgtttttttt

2280

ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac

2340

tcttgcgatc tatggtccct ttaggagttt aggacatcga tttaatttcg gatagttcga

2400

gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc

2460

gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca

2520

gtaagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct

2580

gtctgttatg atcttagtct tgataaggtt cggtcgtgct agctacgtcc tgtgcagcac

2640

ttaattgtca ggtcataatt tttagcatgc ctttttttta ttggtttggt tttgtctgac

2700

tgggctgtag atagtttcaa tctttgtctg actgggctgt agatagtttc aatcttcctg

2760

tctgtttatt ttattaaatt tggatctgta tgtgtgtcat atatcttcat cttttagata

2820

tatcgatagg tatatatgtt gctgtcgttt tttactgttc ctttatgaga tatattcatg

2880

cttagataca tgaaacaacg tgctgttaca gtttaatagt tcttgtttat ctaataaaca

2940

aataaggata ggtgctgcag ttagttttac tggtactttt tttgacatga acctacggct

3000

taataattag tcttcatcaa ataaaaagca tattttttaa ttatttcgat atacttgaat

3060

gatgtcatat gcagcatctg tgtgaatttt tggccctgtc ttcatatgat gtttatttgc

3120

ttgggactgt ttctttggct gataactcac cctgttgttt ggtgatcctt ctgcag

3176

<210> 50

<211> 2043

<212> DNA

<213> Bouteloua gracilis

<400> 50

aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc

60

aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt

120

atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacgggg

180

cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta

240

ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa

300

cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat

360

aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca

420

agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc

480

tcaagttttt ttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat

540

cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg

600

cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt

660

tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat

720

gattaaatca tataaagttt ctaagtcttg tttgacaaga ttttttttaga ttttcatcta

780

aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa

840

aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa

900

ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat

960

gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag

1020

agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg

1080

gtttaccatt ttttgtttga ttttagtttt agagaagcgt ttataacagg acctaaaatc

1140

tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg

1200

aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta

1260

gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa

1320

agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt

1380

gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca

1440

aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt

1500

gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat

1560

ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc

1620

ctctatttta ttccactaga attgattata aatttagcct cttaaatttt ataagttggg

1680

agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc

1740

cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa cggagaacc accgccagcg

1800

ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc

1860

tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc

1920

tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc

1980

ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc

2040

caa

2043

<210> 51

<211> 1042

<212> DNA

<213> Bouteloua gracilis

<400> 51

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg

180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga

240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg

300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat

360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt

420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt

480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt

540

tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg

660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta

720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt

780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt

840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt

900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc

960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg

1020

ttgtttggtg atccttctgc ag

1042

<210> 52

<211> 3139

<212> DNA

<213> Bouteloua gracilis

<400> 52

gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat

60

gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt

120

atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa

180

gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc

240

aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata

300

atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg

360

aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga

420

atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt

480

aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt

540

aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa

600

aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa

660

caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat

720

ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa

780

gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc

840

ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat

900

gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc

960

ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct

1020

aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt

1080

tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc

1140

acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag

1200

gactgagccg gcaaatctag agattctcga agtcactatt ggcacctcgt tatcaacgag

1260

aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata

1320

cgagcacccg taccaagttg aatatttgaa cccgagtggg tagattccac cgcaaaggac

1380

ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt

1440

ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag

1500

cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta

1560

taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc

1620

ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt

1680

caagtgacct cagtgagcc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac

1740

cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc

1800

tccctggcgt ctggtcccct ccggagactt ccgctccacc tcccaccggt ggcggtttcc

1860

gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacgggggg

1920

attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc

1980

ccatccccag cttcttcccc aacctcatct tctcgtgttg ttcggcccaa cccgatcgat

2040

ccccaattcc ctcgtcgtct ctcgtcgcga gcctcgtcga tccccgcttc aaggtacagc

2100

gatcgatcga tcatcctcgc tctctctacc ttctctctct tagggcgtgc tggttctgtt

2160

cctgtttttc catggctgcg aggtacaata gattggcgat tcatggttag ggcctgctag

2220

ttctgttcct gtttttttttt tttccatggc tgcgaggcac aatagatctg atggcgttat

2280

gatggttaac ttgtcatact cttgcgatct atggtccctt taggagttta ggacatcgat

2340

ttaatttcgg atagttcgag atctgtgatc catggttagt accctaggca gtggggttag

2400

atccgtgctg ttatggttcg tagatggatt ctgattgctc agtaactggg aatcctggga

2460

tggttctagc tggttcgcag ataagatcga tttcatgata tgctatatct tgtttggttg

2520

ccgtggttcc gttaaatctg tctgttatga tcttagtctt tgataaggtt cggtcgtgct

2580

agctacgtcc tgtgcagcac ttaattgtca ggtcataatt tttagcatgc ctttttttta

2640

ttggtttggt tttgtctgac tgggctgtag atagtttcaa tctttgtctg actgggctgt

2700

agatagtttc aatcttcctg tctgtttatt ttattaaatt tggatctgta tgtgtgtcat

2760

atatcttcat cttttagata tatcgatagg tatatatgtt gctgtcgttt tttactgttc

2820

ctttatgaga tatattcatg cttagataca tgaaacaacg tgctgttaca gtttaatagt

2880

tcttgtttat ctaataaaca aataaggata ggtgctgcag ttagttttac tggtactttt

2940

tttgacatga acctacggct taataattag tcttcatcaa ataaaaagca tattttttaa

3000

ttatttcgat atacttgaat gatgtcatat gcagcatctg tgtgaatttt tggccctgtc

3060

ttcatatgat gtttatttgc ttgggactgt ttctttggct gataactcac cctgttgttt

3120

ggtgatcctt ctgcaggtg

3139

<210> 53

<211> 2002

<212> DNA

<213> Bouteloua gracilis

<400> 53

gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat

60

gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt

120

atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa

180

gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc

240

aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata

300

atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg

360

aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga

420

atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt

480

aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt

540

aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa

600

aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa

660

caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat

720

ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa

780

gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc

840

ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat

900

gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc

960

ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct

1020

aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt

1080

tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc

1140

acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag

1200

gactgagccg gcaaatctag agattctcga agtcactatt ggcacctcgt tatcaacgag

1260

aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata

1320

cgagcacccg taccaagttg aatatttgaa cccgagtggg tagattccac cgcaaaggac

1380

ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt

1440

ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag

1500

cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta

1560

taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc

1620

ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt

1680

caagtgacct cagtgagcc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac

1740

cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc

1800

tccctggcgt ctggtcccct ccggagactt ccgctccacc tcccaccggt ggcggtttcc

1860

gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacgggggg

1920

attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc

1980

ccatccccag cttcttcccc aa

2002

<210> 54

<211> 1046

<212> DNA

<213> Bouteloua gracilis

<400> 54

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg

180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga

240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg

300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat

360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt

420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg

480

tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt

540

tttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact

600

gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt

660

gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt

720

actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt

780

taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg

840

tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat

900

tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg

960

ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct

1020

gttgtttggt gatccttctg caggtg

1046

<210> 55

<211> 2160

<212> DNA

<213> Bouteloua gracilis

<400> 55

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt

60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat

120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat

180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct

240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta

300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt

360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc

420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag

480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta

540

tttttcatgc cctcctaaaa ttttactccta aaaccctagc tataggagcc tcctatccat

600

cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc

720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc

780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc

840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct

900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc

960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc

1020

ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt

1080

ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc

1140

gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg

1200

cgaggtacaa tagattggcg attcatggtt agggcctgct agttctgttc ctgttttttt

1260

ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac

1320

tcttgcgatc tatggtccct ttaggagttt aggacatcga tttaatttcg gatagttcga

1380

gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc

1440

gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca

1500

gtaagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct

1560

gtctgttatg atcttagtct ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca

1620

cttaattgtc aggtcataat ttttagcatg cctttttttt attggtttgg ttttgtctga

1680

ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct

1740

gtctgtttat tttattaaat ttggatctgt atgtgtgtca tatatcttca tcttttagat

1800

atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat

1860

gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac

1920

aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc

1980

ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa

2040

tgtgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg

2100

cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg

2160

<210> 56

<211> 1024

<212> DNA

<213> Bouteloua gracilis

<400> 56

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt

60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat

120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat

180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct

240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta

300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt

360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc

420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag

480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta

540

tttttcatgc cctcctaaaa ttttactccta aaaccctagc tataggagcc tcctatccat

600

cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc

720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc

780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc

840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct

900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc

960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc

1020

ccaa

1024

<210> 57

<211> 1045

<212> DNA

<213> Bouteloua gracilis

<400> 57

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

120

ctgctagttc tgttcctgtt ttttttttttc catggctgcg aggcacaata gatctgatgg

180

cgttatgatg gttaacttgt catactcttg cgatctatgg tccctttagg agtttaggac

240

atcgatttaa tttcggatag ttcgagatct gtgatccatg gttagtaccc taggcagtgg

300

ggttagatcc gtgctgttat ggttcgtaga tggattctga ttgctcagta actgggaatc

360

ctgggatggt tctagctggt tcgcagataa gatcgatttc atgatatgct atatcttgtt

420

tggttgccgt ggttccgtta aatctgtctg ttatgatctt agtctttgat aaggttcggt

480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt

540

tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg

660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta

720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt

780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt

840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt

900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc

960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg

1020

ttgtttggtg atccttctgc aggtg

1045

<210> 58

<211> 2160

<212> DNA

<213> Bouteloua gracilis

<400> 58

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt

60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat

120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat

180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct

240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta

300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt

360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc

420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag

480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta

540

tttttcatgc cctcctaaaa ttttactccta aaaccctagc tataggagcc tcctatccat

600

cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc

720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc

780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc

840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct

900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc

960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc

1020

ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt

1080

ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc

1140

gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg

1200

cgaggtacaa tagattggcg attcatggtt agggcctgct agttctgttc ctgttttttt

1260

tttttccatg gctgcgaggc acaatagatc tgatggcgtt atgatggtta acttgtcata

1320

ctcttgcgat ctatggtccc tttaggagtt taggacatcg atttaatttc ggatagttcg

1380

agatctgtga tccatggtta gtaccctagg cagtggggtt agatccgtgc tgttatggtt

1440

cgtagatgga ttctgattgc tcagtaactg ggaatcctgg gatggttcta gctggttcgc

1500

agataagatc gatttcatga tatgctatat cttgtttggt tgccgtggtt ccgttaaatc

1560

tgtctgttat gatcttagtc ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca

1620

cttaattgtc aggtcataat ttttagcatg cctttttttt attggtttgg ttttgtctga

1680

ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct

1740

gtctgtttat tttattaaat ttggatctgt atgtgtgtca tatatcttca tcttttagat

1800

atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat

1860

gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac

1920

aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc

1980

ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa

2040

tgtgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg

2100

cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg

2160

<210> 59

<211> 1045

<212> DNA

<213> Bouteloua gracilis

<400> 59

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg

180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga

240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg

300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat

360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt

420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt

480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt

540

tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg

660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta

720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt

780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt

840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt

900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc

960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg

1020

ttgtttggtg atccttctgc aggtg

1045

<210> 60

<211> 1885

<212> DNA

<213> Bouteloua gracilis

<400> 60

caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag

60

taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc

120

aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gccgacaa

180

aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact

240

agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc

300

ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt

360

tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc

420

ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga

480

caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca

540

cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc

600

ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc

660

acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata

720

gccagcccca tccccagctt cttccccaac ctcatcttct cgtgttgttc ggcccaaccc

780

gatcgatccc caattccctc gtcgtctctc gtcgcgagcc tcgtcgatcc ccgcttcaag

840

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg

900

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc

960

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg

1020

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga

1080

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg

1140

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat

1200

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt

1260

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt

1320

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt

1380

tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg

1440

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg

1500

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta

1560

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt

1620

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt

1680

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt

1740

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc

1800

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg

1860

ttgtttggtg atccttctgc aggtg

1885

<210> 61

<211> 749

<212> DNA

<213> Bouteloua gracilis

<400> 61

caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag

60

taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc

120

aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gccgacaa

180

aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact

240

agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc

300

ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt

360

tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc

420

ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga

480

caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca

540

cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc

600

ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc

660

acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata

720

gccagcccca tccccagctt cttccccaa

749

<210> 62

<211> 6813

<212> DNA

<213> Miscanthus sinesis

<400> 62

agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac

60

tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa

120

aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg

180

gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta

240

attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg

300

acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat

360

gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct

420

ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg

480

taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt

540

acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc

600

ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg

660

acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc

720

ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag

780

gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc

840

cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag

900

cagcgacaga ttcagcagcg agagaccggc taccgtagca ttttcatttt tatttgataa

960

ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa

1020

ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa

1080

cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt

1140

atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat

1200

catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac

1260

gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg

1320

catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt

1380

tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc

1440

tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc

1500

gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc

1560

tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg

1620

gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc

1680

gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga

1740

agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta

1800

caagtcagag aaagagag aatgtatacg tgtgctatct agtcttgttg cccacgctgt

1860

caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg

1920

ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga

1980

cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt

2040

gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt

2100

atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca

2160

gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga

2220

tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg

2280

atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg

2340

tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg

2400

gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt

2460

acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc

2520

cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta

2580

cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc

2640

tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa

2700

aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga

2760

cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct

2820

cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga

2880

actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca

2940

tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct

3000

agctaatctt tttgttttat ttggtttccc tttgaaatct tctaatttag ctttcataga

3060

aataatctag gtatttttta ttttatatgt tctatctgtt tgcattaatt ttgatcattt

3120

gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg

3180

ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt

3240

tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg

3300

aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta

3360

aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt

3420

gcgtgcgttc tcaagttttc tttcttgcaa attacatttt ttttaaaaaa aagtataatt

3480

tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc

3540

tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc

3600

taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca

3660

acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag

3720

cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag

3780

tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat

3840

attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa

3900

caaacttatt tgaagaaaca aatgttgttc atatatttct atatacgaat accatagcga

3960

cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag

4020

ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac

4080

attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag

4140

gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt

4200

gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc

4260

ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa

4320

atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt

4380

gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc

4440

tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg

4500

acaagtctaa cggacaccaa cggcgaacc accagcgccg agccaagcga agcagactgc

4560

agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc

4620

tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt

4680

ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc

4740

ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc

4800

ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac

4860

ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct

4920

accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct

4980

gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat

5040

gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc

5100

taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt

5160

ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga

5220

tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt

5280

gctagctacg tcctgtgcac ttaattgtca ggtcataatt tttactatac tttttttttg

5340

gtttggtttg gtttcgtctg atttggctgt cgttctagat cagagtagaa actgtttcaa

5400

actacctgtt ggatttatta aggtagcgtt tggttcctgg tatcgaatca tacacgcacc

5460

agtgcatctt ggatagccag ctggggccca cctgtccaac cgtttggttg ccggatcgaa

5520

cgagtccatt caagaccgaa ccatgcagag caatcgaata ttctcttgtg acgctgtatc

5580

atccagttcg gcaaaaaaca ccgaatgccg ccatacagga caccgtactg agcgtctgca

5640

actctgcatc ccgctcactg ctcacatctc cgcttgccgc ctcacccatc cgactcagac

5700

cagagccaca cggattactg ctgctggtgt gtgtattaac aaaagatcca tttgaccggga

5760

gcacatgcag cttggatgga aaaaatttat tatattcgtc agtgctgcat atgtactcat

5820

acttgcatga tggttttatt tattcgacct catcagtcct ggcactatgg aaagtcattg

5880

tagtatagat tttttaatat aatataaatc attggtgact tatcttgctt aattttattt

5940

tcttattatg aaatatcgtt gcattcataa tagcaaattt gtgcaaatat atagaatcta

6000

cgtgaaattc ttggttggac caatacaaca aacccctcaa acattctctt gtactgaacc

6060

ataccattcc gtacaaccat ccaaacaaaa atcatgtatc atcatgtaca tgtaaccaaa

6120

caattaacac gcaccatcct attcagactt gtctcatcca taatctatcc atccaggatg

6180

atccatccca ttcatctata tacacccaat caaacgctac ctaaaatttg gatctgtatg

6240

tgtcacatat atcttaataa gatggatgga aatatctctt tatcttttag atatggatag

6300

gtatatatgt tgctgtgggt ttgttagtta tatatatacg tgcttacata cgtgaagaaa

6360

cctgctgcta cagtttaata attcttgttc atctcaacaa ataacgatag gcgtatatgt

6420

tgctgtgttt tttactggta ctttgttaga tatatacatg cttacataca tgaagaacac

6480

atgctacagt tcaaaaattc ttgttcatct cataaacaaa aaggaggtgt atatgttgct

6540

gtgggtttta ctggtacttt attagatata tacatgctta catagatgaa gcaacatgct

6600

gctatggtgt ttaataatta ttgtttatct aataaacaaa catgcttttt aattatcttg

6660

atatgtttgg atgatggcat atgcagcagc tatgtgtgga ttttaaatac ccagcatcat

6720

gagcatgcat gaccctgcct tagtatgcag ttatttgctt gagactgttt cttttgttga

6780

tactcatcct ttagttcggt cactcttctg cag

6813

<210> 63

<211> 5359

<212> DNA

<213> Miscanthus sinesis

<400> 63

agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac

60

tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa

120

aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg

180

gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta

240

attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg

300

acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat

360

gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct

420

ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg

480

taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt

540

acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc

600

ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg

660

acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc

720

ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag

780

gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc

840

cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag

900

cagcgacaga ttcagcagcg agagaccggc taccgtagca ttttcatttt tatttgataa

960

ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa

1020

ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa

1080

cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt

1140

atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat

1200

catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac

1260

gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg

1320

catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt

1380

tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc

1440

tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc

1500

gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc

1560

tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg

1620

gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc

1680

gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga

1740

agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta

1800

caagtcagag aaagagag aatgtatacg tgtgctatct agtcttgttg cccacgctgt

1860

caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg

1920

ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga

1980

cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt

2040

gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt

2100

atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca

2160

gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga

2220

tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg

2280

atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg

2340

tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg

2400

gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt

2460

acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc

2520

cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta

2580

cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc

2640

tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa

2700

aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga

2760

cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct

2820

cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga

2880

actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca

2940

tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct

3000

agctaatctt tttgttttat ttggtttccc tttgaaatct tctaatttag ctttcataga

3060

aataatctag gtatttttta ttttatatgt tctatctgtt tgcattaatt ttgatcattt

3120

gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg

3180

ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt

3240

tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg

3300

aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta

3360

aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt

3420

gcgtgcgttc tcaagttttc tttcttgcaa attacatttt ttttaaaaaa aagtataatt

3480

tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc

3540

tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc

3600

taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca

3660

acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag

3720

cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag

3780

tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat

3840

attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa

3900

caaacttatt tgaagaaaca aatgttgttc atatatttct atatacgaat accatagcga

3960

cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag

4020

ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac

4080

attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag

4140

gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt

4200

gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc

4260

ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa

4320

atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt

4380

gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc

4440

tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg

4500

acaagtctaa cggacaccaa cggcgaacc accagcgccg agccaagcga agcagactgc

4560

agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc

4620

tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt

4680

ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc

4740

ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc

4800

ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac

4860

ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct

4920

accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct

4980

gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat

5040

gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc

5100

taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt

5160

ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga

5220

tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt

5280

gctagctacg tcctgtgcac ttaattgtca ggtcataatt tttactatac tttttttttg

5340

gtttggtttg gtttcgtct

5359

<210> 64

<211> 63

<212> DNA

<213> Miscanthus sinesis

<400> 64

gatttggctg tcgttctaga tcagagtaga aactgtttca aactacctgt tggatttatt

60

aag

63

<210> 65

<211> 1391

<212> DNA

<213> Miscanthus sinesis

<400> 65

gtagcgtttg gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct

60

ggggcccacc tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc

120

atgcagagca atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc

180

gaatgccgcc atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct

240

cacatctccg cttgccgcct cacccatccg actcagacca gagccacacg gattactgct

300

gctggtgtgt gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa

360

aaatttatta tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta

420

ttcgacctca tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa

480

tataaatcat tggtgactta tcttgcttaa tttttattttc ttattatgaa atatcgttgc

540

attcataata gcaaatttgt gcaaatatat agaatctacg tgaaattctt ggttggacca

600

atacaacaaa cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc

660

aaacaaaaat catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat

720

tcagacttgt ctcatccata atctatccat ccaggatgat ccatcccatt catctatata

780

cacccaatca aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga

840

tggatggaaa tatctcttta tcttttagat atggataggt atatatgttg ctgtgggttt

900

gttagttata tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat

960

tcttgttcat ctcaacaaat aacgataggc gtatatgttg ctgtgtttt tactggtact

1020

ttgttagata tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt

1080

gttcatctca taaacaaaaa ggaggtgtat atgttgctgt gggttttact ggtactttat

1140

tagatatata catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt

1200

gtttatctaa taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat

1260

gcagcagcta tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta

1320

gtatgcagtt atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca

1380

ctcttctgca g

1391

<210> 66

<211> 4402

<212> DNA

<213> Miscanthus sinesis

<400> 66

cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg

60

cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc

120

agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga

180

cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt

240

ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga

300

cgtccgga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg

360

ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac

420

ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga

480

ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc

540

gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg

600

ttttatttgg ttttccctttg aaatcttcta atttagcttt catagaaata atctaggtat

660

tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt

720

ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt

780

ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct

840

aactattact ccgtatatga gtgagttgtt aaattttattc caggtgaaat ggcattaacg

900

atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc

960

atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa

1020

gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt

1080

ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata

1140

aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt

1200

gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc

1260

tcacatgact aaatgatata aggttgctaa ggtctttctt gataagcttt tttatgaatt

1320

tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc

1380

gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag

1440

tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa

1500

gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa

1560

tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc

1620

caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc

1680

tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta

1740

gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct

1800

tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt

1860

ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg

1920

gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt

1980

tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc

2040

ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga

2100

caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg

2160

agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct

2220

ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg

2280

ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg

2340

ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaaatagc

2400

cagccccatc cctcgtctct cgtgttgttc ggagcgcaca cacaacccga tccccaatca

2460

atcgatcccc gcttcaaggt acggcgatcc tcctccctct ctctctacct tctcttctct

2520

acactagatc ggcggtccat ggttagggcc tgctagttcc gttcctgttt ttccatggct

2580

gcgaggtaca atagatctga tggcgttatg atggttaact tgtcatgctt ttgcgattta

2640

tagtcccttt agatagttcg agatcggtga tccatggtta gtaccctagg ctgtggagtc

2700

gggttagatc cgcgctgtta gggttcgtat atggaggcga gctgttctga ttgttaactt

2760

gctgggaatc ctgggatggt tctagctgtt ccgcagatga gatcgatttc atgatctgct

2820

gtatctatcc gtggtatgat gttagccttt gatatggttc gatcgtgcta gctacgtcct

2880

gtgcacttaa ttgtcaggtc ataattttta ctatactttt ttttggtttt ggtttggttt

2940

cgtctgattt ggctgtcgtt ctagatcaga gtagaaactg tttcaaacta cctgttggat

3000

ttattaaggt agcgtttggt tcctggtatc gaatcataca cgcaccagtg catcttggat

3060

agccagctgg ggcccacctg tccaaccgtt tggttgccgg atcgaacgag tccattcaag

3120

accgaaccat gcagagcaat cgaatattct cttgtgacgc tgtatcatcc agttcggcaa

3180

aaaacaccga atgccgccat acaggacacc gtactgagcg tctgcaactc tgcatcccgc

3240

tcactgctca catctccgct tgccgcctca cccatccgac tcagaccaga gccacacgga

3300

ttactgctgc tggtgtgtgt attaacaaaa gatccatttg accggagcac atgcagcttg

3360

gatggaaaaa atttattata ttcgtcagtg ctgcatatgt actcatactt gcatgatggt

3420

tttatttatt cgacctcatc agtcctggca ctatggaaag tcattgtagt atagattttt

3480

taatataata taaatcattg gtgacttatc ttgcttaatt ttatttttctt attatgaaat

3540

atcgttgcat tcataatagc aaatttgtgc aaatatatag aatctacgtg aaattcttgg

3600

ttggaccaat acaacaaacc cctcaaacat tctcttgtac tgaaccatac cattccgtac

3660

aaccatccaa acaaaaatca tgtatcatca tgtacatgta accaaacaat taacacgcac

3720

catcctattc agacttgtct catccataat ctatccatcc aggatgatcc atcccattca

3780

tctatataca cccaatcaaa cgctacctaa aatttggatc tgtatgtgtc acatatatctct

3840

taataagatg gatggaaata tctctttatc ttttagatat ggataggtat atatgttgct

3900

gtgggtttgt tagttata tatacgtgct tacatacgtg aagaaacctg ctgctacagt

3960

ttaataattc ttgttcatct caacaaataa cgataggcgt atatgttgct gtgtttttta

4020

ctggtacttt gttagatata tacatgctta catacatgaa gaacacatgc tacagttcaa

4080

aaattcttgt tcatctcata aacaaaaagg aggtgtatat gttgctgtgg gttttactgg

4140

tactttatta gatatataca tgcttacata gatgaagcaa catgctgcta tggtgtttaa

4200

taattattgt ttatctaata aacaaacatg cttttttaatt atcttgatat gtttggatga

4260

tggcatatgc agcagctatg tgtggatttt aaatacccag catcatgagc atgcatgacc

4320

ctgccttagt atgcagttat ttgcttgaga ctgtttcttt tgttgatact catcctttag

4380

ttcggtcact cttctgcagg tg

4402

<210> 67

<211> 2423

<212> DNA

<213> Miscanthus sinesis

<400> 67

cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg

60

cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc

120

agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga

180

cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt

240

ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga

300

cgtccgga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg

360

ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac

420

ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga

480

ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc

540

gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg

600

ttttatttgg ttttccctttg aaatcttcta atttagcttt catagaaata atctaggtat

660

tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt

720

ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt

780

ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct

840

aactattact ccgtatatga gtgagttgtt aaattttattc caggtgaaat ggcattaacg

900

atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc

960

atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa

1020

gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt

1080

ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata

1140

aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt

1200

gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc

1260

tcacatgact aaatgatata aggttgctaa ggtctttctt gataagcttt tttatgaatt

1320

tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc

1380

gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag

1440

tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa

1500

gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa

1560

tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc

1620

caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc

1680

tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta

1740

gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct

1800

tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt

1860

ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg

1920

gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt

1980

tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc

2040

ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga

2100

caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg

2160

agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct

2220

ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg

2280

ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg

2340

ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaaatagc

2400

cagccccatc cctcgtctct cgt

2423

<210> 68

<211> 55

<212> DNA

<213> Miscanthus sinesis

<400> 68

gttgttcgga gcgcacacac aacccgatcc ccaatcaatc gatccccgct tcaag

55

<210> 69

<211> 1924

<212> DNA

<213> Miscanthus sinesis

<400> 69

gtacggcgat ccctcctccct ctctctctac cttctcttct ctacactaga tcggcggtcc

60

atggttaggg cctgctagtt ccgttcctgt ttttccatgg ctgcgaggta caatagatct

120

gatggcgtta tgatggttaa cttgtcatgc ttttgcgatt tatagtccct ttagatagtt

180

cgagatcggt gatccatggt tagtacccta ggctgtggag tcgggttaga tccgcgctgt

240

tagggttcgt atatggaggc gagctgttct gattgttaac ttgctgggaa tcctgggatg

300

gttctagctg ttccgcagat gagatcgatt tcatgatctg ctgtatctat ccgtggtatg

360

atgttagcct ttgatatggt tcgatcgtgc tagctacgtc ctgtgcactt aattgtcagg

420

tcataatttt tactatactt ttttttttggt ttggtttggt ttcgtctgat ttggctgtcg

480

ttctagatca gagtagaaac tgtttcaaac tacctgttgg atttattaag gtagcgtttg

540

gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct ggggcccacc

600

tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc atgcagagca

660

atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc gaatgccgcc

720

atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct cacatctccg

780

cttgccgcct cacccatccg actcagacca gagccacacg gattactgct gctggtgtgt

840

gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa aaatttatta

900

tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta ttcgacctca

960

tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa tataaatcat

1020

tggtgactta tcttgcttaa tttttattttc ttattatgaa atatcgttgc attcataata

1080

gcaaatttgt gcaaattat agaatctacg tgaaattctt ggttggacca atacaacaaa

1140

cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc aaacaaaaat

1200

catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat tcagacttgt

1260

ctcatccata atctatccat ccaggatgat ccatcccatt catctatata cacccaatca

1320

aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga tggatggaaa

1380

tatctcttta tcttttagat atggataggt atatatgttg ctgtgggttt gttagttata

1440

tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat tcttgttcat

1500

ctcaacaaat aacgataggc gtatatgttg ctgtgttttt tactggtact ttgttagata

1560

tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt gttcatctca

1620

taaacaaaaa ggaggtgtat atgttgctgt gggttttact ggtactttat tagatatata

1680

catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt gtttatctaa

1740

taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat gcagcagcta

1800

tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta gtatgcagtt

1860

atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca ctcttctgca

1920

ggtg

1924

<210> 70

<211> 3426

<212> DNA

<213> Miscanthus sinesis

<400> 70

gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa

60

attacatttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt

120

gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct

180

gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt

240

ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga

300

tataaggttg ctaaggtctt tcttgataag cttttttatg aatttcatct aaattttcga

360

gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt

420

taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca

480

ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc

540

atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc

600

tttgtgccgc tttgagtgtc gctttggcag ctagtaccta tgtccacctt cacagcttgt

660

gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg

720

gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac

780

tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa

840

tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc

900

ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc

960

tccacaataa ctttggtttt actatttttt gtttgatttt agtttcagaa gtccactttt

1020

gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg

1080

taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa cggcgaacc

1140

accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg

1200

gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac

1260

ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc

1320

tctcacacgg cacgaaaccg tcacggcacc ggcagcacag cacgggggat tcctttccca

1380

ccgctccgtc ccttttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt

1440

ctctcgtgtt gttcggagcg cacacacaac ccgatcccca atcaatcgat ccccgcttca

1500

aggtacggcg atcctcctcc ctctctctct accttctctt ctctacacta gatcggcggt

1560

ccatggttag ggcctgctag ttccgttcct gtttttccat ggctgcgagg tacaatagat

1620

ctgatggcgt tatgatggtt aacttgtcat gcttttgcga tttatagtcc ctttagatag

1680

ttcgagatcg gtgatccatg gttagtaccc taggctgtgg agtcgggtta gatccgcgct

1740

gttagggttc gtatatggag gcgagctgtt ctgattgtta acttgctggg aatcctggga

1800

tggttctagc tgttccgcag atgagatcga tttcatgatc tgctgtatct atccgtggta

1860

tgatgttagc ctttgatatg gttcgatcgt gctagctacg tcctgtgcac ttaattgtca

1920

ggtcataatt tttactatac ttttttttttg gtttggtttg gtttcgtctg atttggctgt

1980

cgttctagat cagagtagaa actgtttcaa actacctgtt ggatttatta aggtagcgtt

2040

tggttcctgg tatcgaatca tacacgcacc agtgcatctt ggatagccag ctggggccca

2100

cctgtccaac cgtttggttg ccggatcgaa cgagtccatt caagaccgaa ccatgcagag

2160

caatcgaata ttctcttgtg acgctgtatc atccagttcg gcaaaaaaca ccgaatgccg

2220

ccatacagga caccgtactg agcgtctgca actctgcatc ccgctcactg ctcacatctc

2280

cgcttgccgc ctcacccatc cgactcagac cagagccaca cggattactg ctgctggtgt

2340

gtgtattaac aaaagatcca tttgaccgga gcacatgcag cttggatgga aaaaatttat

2400

tatattcgtc agtgctgcat atgtactcat acttgcatga tggttttatt tattcgacct

2460

catcagtcct ggcactatgg aaagtcattg tagtatagat tttttaatat aatataaatc

2520

attggtgact tatcttgctt aattttattt tcttattatg aaatatcgtt gcattcataa

2580

tagcaaattt gtgcaaatat atagaatcta cgtgaaattc ttggttggac caatacaaca

2640

aacccctcaa acattctctt gtactgaacc ataccattcc gtacaaccat ccaaacaaaa

2700

atcatgtatc atcatgtaca tgtaaccaaa caattaacac gcaccatcct attcagactt

2760

gtctcatcca taatctatcc atccaggatg atccatccca ttcatctata tacacccaat

2820

caaacgctac ctaaaatttg gatctgtatg tgtcacatat atcttaataa gatggatgga

2880

aatatctctt tatcttttag atatggatag gtatatatgt tgctgtgggt ttgttagtta

2940

tatatatacg tgcttacata cgtgaagaaa cctgctgcta cagtttaata attcttgttc

3000

atctcaacaa ataacgatag gcgtatatgt tgctgtgttt tttactggta ctttgttaga

3060

tatatacatg cttacataca tgaagaacac atgctacagt tcaaaaattc ttgttcatct

3120

cataaacaaa aaggaggtgt atatgttgct gtgggtttta ctggtacttt attagatata

3180

tacatgctta catagatgaa gcaacatgct gctatggtgt ttaataatta ttgtttatctct

3240

aataaacaaa catgcttttt aattatcttg atatgtttgg atgatggcat atgcagcagc

3300

tatgtgtgga ttttaaatac ccagcatcat gagcatgcat gaccctgcct tagtatgcag

3360

ttatttgctt gagactgttt cttttgttga tactcatcct ttagttcggt cactcttctg

3420

caggtg

3426

<210> 71

<211> 1447

<212> DNA

<213> Miscanthus sinesis

<400> 71

gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa

60

attacatttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt

120

gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct

180

gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt

240

ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga

300

tataaggttg ctaaggtctt tcttgataag cttttttatg aatttcatct aaattttcga

360

gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt

420

taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca

480

ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc

540

atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc

600

tttgtgccgc tttgagtgtc gctttggcag ctagtaccta tgtccacctt cacagcttgt

660

gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg

720

gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac

780

tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa

840

tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc

900

ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc

960

tccacaataa ctttggtttt actatttttt gtttgatttt agtttcagaa gtccactttt

1020

gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg

1080

taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa cggcgaacc

1140

accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg

1200

gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac

1260

ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc

1320

tctcacacgg cacgaaaccg tcacggcacc ggcagcacag cacgggggat tcctttccca

1380

ccgctccgtc ccttttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt

1440

ctctcgt

1447

<210> 72

<211> 2878

<212> DNA

<213> Miscanthus sinesis

<400> 72

ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc

60

gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta

120

cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa

180

acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg

240

ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac

300

aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa

360

aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat

420

aactttggtt ttactatttt ttgtttgatt ttagtttcag aagtccactt ttgtacgtgc

480

tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa

540

atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc

600

cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca

660

acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct

720

ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac

780

ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg

840

tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgtg

900

ttgttcggag cgcacacaca acccgatccc caatcaatcg atccccgctt caaggtacgg

960

cgatcctcct ccctctctct ctaccttctc ttctctacac tagatcggcg gtccatggtt

1020

agggcctgct agttccgttc ctgtttttcc atggctgcga ggtacaatag atctgatggc

1080

gttatgatgg ttaacttgtc atgcttttgc gatttatagt ccctttagat agttcgagat

1140

cggtgatcca tggttagtac cctaggctgt ggagtcgggt tagatccgcg ctgttagggt

1200

tcgtatatgg aggcgagctg ttctgattgt taacttgctg ggaatcctgg gatggttcta

1260

gctgttccgc agatgagatc gatttcatga tctgctgtat ctatccgtgg tatgatgtta

1320

gcctttgata tggttcgatc gtgctagcta cgtcctgtgc acttaattgt caggtcataa

1380

tttttactat actttttttt tggtttggtt tggtttcgtc tgatttggct gtcgttctag

1440

atcagagtag aaactgtttc aaactacctg ttggatttat taaggtagcg tttggttcct

1500

ggtatcgaat catacacgca ccagtgcatc ttggatagcc agctggggcc cacctgtcca

1560

accgtttggt tgccggatcg aacgagtcca ttcaagaccg aaccatgcag agcaatcgaa

1620

tattctcttg tgacgctgta tcatccagtt cggcaaaaaa caccgaatgc cgccatacag

1680

gacaccgtac tgagcgtctg caactctgca tcccgctcac tgctcacatc tccgcttgcc

1740

gcctcaccca tccgactcag accagagcca cacggattac tgctgctggt gtgtgtatta

1800

acaaaagatc catttgaccg gagcacatgc agcttggatg gaaaaaattt attatattcg

1860

tcagtgctgc atatgtactc atacttgcat gatggtttta tttattcgac ctcatcagtc

1920

ctggcactat ggaaagtcat tgtagtatag attttttaat ataatataaa tcattggtga

1980

cttatcttgc ttaattttat tttcttatta tgaaatatcg ttgcattcat aatagcaaat

2040

ttgtgcaaat atatagaatc tacgtgaaat tcttggttgg accaatacaa caaacccctc

2100

aaacattctc ttgtactgaa ccataccatt ccgtacaacc atccaaacaa aaatcatgta

2160

tcatcatgta catgtaacca aacaattaac acgcaccatc ctattcagac ttgtctcatc

2220

cataatctat ccatccagga tgatccatcc cattcatcta tatacaccca atcaaacgct

2280

acctaaaatt tggatctgta tgtgtcacat atatcttaat aagatggatg gaaatatctcc

2340

tttatctttt agatatggat aggtatatat gttgctgtgg gtttgttagt tatatatata

2400

cgtgcttaca tacgtgaaga aacctgctgc tacagtttaa taattcttgt tcatctcaac

2460

aaataacgat aggcgtatat gttgctgtgt tttttactgg tactttgtta gatatataca

2520

tgcttacata catgaagaac acatgctaca gttcaaaaat tcttgttcat ctcataaaca

2580

aaaaggaggt gtatatgttg ctgtgggttt tactggtact ttattagata tatacatgct

2640

tacatagatg aagcaacatg ctgctatggt gtttaataat tattgtttat ctaataaaca

2700

aacatgcttt ttaattatct tgatatgttt ggatgatggc atatgcagca gctatgtgtg

2760

gattttaaat acccagcatc atgagcatgc atgaccctgc cttagtatgc agttatttgc

2820

ttgagactgt ttcttttgtt gatactcatc ctttagttcg gtcactcttc tgcaggtg

2878

<210> 73

<211> 899

<212> DNA

<213> Miscanthus sinesis

<400> 73

ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc

60

gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta

120

cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa

180

acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg

240

ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac

300

aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa

360

aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat

420

aactttggtt ttactatttt ttgtttgatt ttagtttcag aagtccactt ttgtacgtgc

480

tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa

540

atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc

600

cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca

660

acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct

720

ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac

780

ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg

840

tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgt

899

<210> 74

<211> 2670

<212> DNA

<213> Miscanthus sinesis

<400> 74

gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat

60

tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat

120

cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg

180

ttttggtggt ttttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga

240

ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca

300

aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt

360

ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg

420

cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga

480

gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt

540

ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc

600

acagcacggg ggattccttt cccaccgctc cgtccctttc tcttcctcgc ccgcccgtta

660

taaatagcca gccccatccc tcgtctctcg tgttgttcgg agcgcacaca caacccgatc

720

cccaatcaat cgatccccgc ttcaaggtac ggcgatcctc ctccctctct ctctaccttc

780

tcttctctac actagatcgg cggtccatgg ttagggcctg ctagttccgt tcctgttttt

840

ccatggctgc gaggtacaat agatctgatg gcgttatgat ggttaacttg tcatgctttt

900

gcgatttata gtccctttag atagttcgag atcggtgatc catggttagt accctaggct

960

gtggagtcgg gttagatccg cgctgttagg gttcgtatat ggaggcgagc tgttctgatt

1020

gttaacttgc tgggaatcct gggatggttc tagctgttcc gcagatgaga tcgatttcat

1080

gatctgctgt atctatccgt ggtatgatgt tagcctttga tatggttcga tcgtgctagc

1140

tacgtcctgt gcacttaatt gtcaggtcat aatttttact atacttttt tttggtttgg

1200

tttggtttcg tctgatttgg ctgtcgttct agatcagagt agaaactgtt tcaaactacc

1260

tgttggattt attaaggtag cgtttggttc ctggtatcga atcatacacg caccagtgca

1320

tcttggatag ccagctgggg cccacctgtc caaccgtttg gttgccggat cgaacgagtc

1380

cattcaagac cgaaccatgc agagcaatcg aatattctct tgtgacgctg tatcatccag

1440

ttcggcaaaa aacaccgaat gccgccatac aggacaccgt actgagcgtc tgcaactctg

1500

catcccgctc actgctcaca tctccgcttg ccgcctcacc catccgactc agaccagagc

1560

cacacggatt actgctgctg gtgtgtgtat taacaaaaga tccatttgac cggagcacat

1620

gcagcttgga tggaaaaaat ttattatatt cgtcagtgct gcatatgtac tcatacttgc

1680

atgatggttt tatttattcg acctcatcag tcctggcact atggaaagtc attgtagtat

1740

agatttttta atataatata aatcattggt gacttatctt gcttaatttt attttcttat

1800

tatgaaatat cgttgcattc ataatagcaa atttgtgcaa atatatagaa tctacgtgaa

1860

attcttggtt ggaccaatac aacaaacccc tcaaacattc tcttgtactg aaccatacca

1920

ttccgtacaa ccatccaaac aaaaatcatg tatcatcatg tacatgtaac caaacaatta

1980

acacgcacca tcctattcag acttgtctca tccataatct atccatccag gatgatccat

2040

cccattcatc tatatacacc caatcaaacg ctacctaaaa tttggatctg tatgtgtcac

2100

atatatctta ataagatgga tggaaatatc tctttatctt ttagatatgg ataggtatat

2160

atgttgctgt gggtttgtta gttatatata tacgtgctta catacgtgaa gaaacctgct

2220

gctacagttt aataattctt gttcatctca acaaataacg ataggcgtat atgttgctgt

2280

gttttttact ggtactttgt tagatatata catgcttaca tacatgaaga acacatgcta

2340

cagttcaaaa attcttgttc atctcataaa caaaaaggag gtgtatatgt tgctgtgggt

2400

tttactggta ctttattaga tatatacatg cttacataga tgaagcaaca tgctgctatg

2460

gtgtttaata attattgttt atctaataaa caaacatgct ttttaattat cttgatatgt

2520

ttggatgatg gcatatgcag cagctatgtg tggattttaa atacccagca tcatgagcat

2580

gcatgaccct gccttagtat gcagttattt gcttgagact gtttcttttg ttgatactca

2640

tcctttagtt cggtcactct tctgcaggtg

2670

<210> 75

<211> 691

<212> DNA

<213> Miscanthus sinesis

<400> 75

gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat

60

tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat

120

cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg

180

ttttggtggt ttttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga

240

ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca

300

aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt

360

ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg

420

cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga

480

gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt

540

ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc

600

acagcacggg ggattccttt cccaccgctc cgtccctttc tcttcctcgc ccgcccgtta

660

taaatagcca gccccatccc tcgtctctcg t

691

<210> 76

<211> 2485

<212> DNA

<213> Miscanthus sinesis

<400> 76

gtggttttga tttctagcct ccacaataac tttggtttta ctattttttg tttgatttta

60

gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg

120

accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac

180

ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg

240

ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc

300

gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc

360

ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc

420

acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat

480

agccagcccc atccctcgtc tctcgtgttg ttcggagcgc acacacaacc cgatccccaa

540

tcaatcgatc cccgcttcaa ggtacggcga tcctcctccc tctctctcta ccttctcttc

600

tctacactag atcggcggtc catggttagg gcctgctagt tccgttcctg tttttccatg

660

gctgcgaggt acaatagatc tgatggcgtt atgatggtta acttgtcatg cttttgcgat

720

ttatagtccc tttagatagt tcgagatcgg tgatccatgg ttagtaccct aggctgtgga

780

gtcgggttag atccgcgctg ttagggttcg tatatggagg cgagctgttc tgattgttaa

840

cttgctggga atcctgggat ggttctagct gttccgcaga tgagatcgat ttcatgatct

900

gctgtatcta tccgtggtat gatgttagcc tttgatatgg ttcgatcgtg ctagctacgt

960

cctgtgcact taattgtcag gtcataattt ttactatact ttttttttgg tttggtttgg

1020

tttcgtctga tttggctgtc gttctagatc agagtagaaa ctgtttcaaa ctacctgttg

1080

gatttattaa ggtagcgttt ggttcctggt atcgaatcat acacgcacca gtgcatcttg

1140

gatagccagc tggggcccac ctgtccaacc gtttggttgc cggatcgaac gagtccattc

1200

aagaccgaac catgcagagc aatcgaatat tctcttgtga cgctgtatca tccagttcgg

1260

caaaaaacac cgaatgccgc catacaggac accgtactga gcgtctgcaa ctctgcatcc

1320

cgctcactgc tcacatctcc gcttgccgcc tcacccatcc gactcagacc agagccacac

1380

ggattactgc tgctggtgtg tgtattaaca aaagatccat ttgaccggag cacatgcagc

1440

ttggatggaa aaaatttatt atattcgtca gtgctgcata tgtactcata cttgcatgat

1500

ggttttattt attcgacctc atcagtcctg gcactatgga aagtcattgt agtatagatt

1560

ttttaatata atataaatca ttggtgactt atcttgctta attttatttt cttattatga

1620

aatatcgttg cattcataat agcaaatttg tgcaaatata tagaatctac gtgaaattct

1680

tggttggacc aatacaacaa acccctcaaa cattctcttg tactgaacca taccattccg

1740

tacaaccatc caaacaaaaa tcatgtatca tcatgtacat gtaaccaaac aattaacacg

1800

caccatccta ttcagacttg tctcatccat aatctatcca tccaggatga tccatcccat

1860

tcatctatat acacccaatc aaacgctacc taaaatttgg atctgtatgt gtcacatata

1920

tcttaataag atggatggaa atatctcttt atcttttaga tatggatagg tatatatgtt

1980

gctgtgggtt tgttagttat atatatacgt gcttacatac gtgaagaaac ctgctgctac

2040

agtttaataa ttcttgttca tctcaacaaa taacgatagg cgtatatgtt gctgtgtttt

2100

ttactggtac tttgttagat atatacatgc ttacatacat gaagaacaca tgctacagtt

2160

caaaaattct tgttcatctc ataaacaaaa aggaggtgta tatgttgctg tgggttttac

2220

tggtacttta ttagatatat acatgcttac atagatgaag caacatgctg ctatggtgtt

2280

taataattat tgtttatcta ataaacaaac atgcttttta attatcttga tatgtttgga

2340

tgatggcata tgcagcagct atgtgtggat tttaaatacc cagcatcatg agcatgcatg

2400

accctgcctt agtatgcagt tatttgcttg agactgtttc ttttgttgat actcatcctt

2460

tagttcggtc actcttctgc aggtg

2485

<210> 77

<211> 506

<212> DNA

<213> Miscanthus sinesis

<400> 77

gtggttttga tttctagcct ccacaataac tttggtttta ctattttttg tttgatttta

60

gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg

120

accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac

180

ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg

240

ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc

300

gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc

360

ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc

420

acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat

480

agccagcccc atccctcgtc tctcgt

506

<210> 78

<211> 4079

<212> DNA

<213> Schizachyium scoparium

<400> 78

ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag

60

agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga

120

gtgaattgat aaatttattc taggtgacat ggccttaacg atggccagta cgtggttaaa

180

tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca

240

ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt

300

tcttgtaaat tacgcttttc tcaaaaaaat tggatcatgc atttatttca ctctaggtgt

360

gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct

420

gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc

480

atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta

540

catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt

600

ttttgagtga aataataaaa ttttaaagca atgttacaag agaagctctg gagataaaag

660

ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat

720

tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt

780

acacgacgct ggcgcttatg tggacgttaa tcggtaattc ttcattttat tttattttgt

840

tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc

900

cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat

960

tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa

1020

catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa

1080

catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca

1140

aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac

1200

acgctcatgc catccatatc attcaacttt tgaatcgttt actatacaat attatttcta

1260

aatttggctg taaagatggc attgatttca taaatatgaa aaataccaaa ttgcacattt

1320

tctttctata ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca

1380

tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga

1440

agtaattttc aatactaaga aacaacaaaa ttttggttttt ttttgtttgg tttcgattat

1500

agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca

1560

tttaatattg taactgattc aagctgagta atagatgaga tgagtgtgtt aggatgtgta

1620

gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta

1680

tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac

1740

atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg

1800

cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt

1860

tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat

1920

tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt

1980

ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt

2040

aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa

2100

gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg

2160

atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag

2220

ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa

2280

cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg

2340

aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc

2400

ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag

2460

atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat

2520

taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt

2580

atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga

2640

caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt

2700

ccgcttcctc ctccgacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt

2760

tcccaccgct ccttcctttt cccttcatcg cccgcagcta taaatagcca cccccgtccg

2820

caacttcttt ccccaacctc atcttttgtt cggagcacgc acacaatccg atcgatcccc

2880

aatcccctcg tctctcctcg cgagcctcgt cgatccgcca ttcaaggtac ggcgatcatc

2940

ctccctccct ctctacctgc tcttctgtag atcggcgacc ccatccatgg ttagggcctg

3000

ctagttctgt tcctgttttt tttccatggc tgcgaggtag aatagatctg atggcgttat

3060

gatggttaat ttgtcatact cttgcggtct atgggtccct ttaggtcatc aatttaattt

3120

tgggtggttg agatcggtga tccatggtta gtaccctagt cagtggggtt ggatccgtgc

3180

tattagggtt cgtagatgga ttctgatggc tcagtaactg ggaatcctag gatggttcca

3240

tctggtttgc agatgagaac gatttcatca tctgctatat cttgtttcgt tgcgtaggtt

3300

ctgtttaaac taatccgtgg tatgatgtta gcctttgata aggttgattt catcatctgc

3360

tatatcttgt ttcgttgcgt aggttctgtt taaactaatc cgtggtatga tgttagcctt

3420

tgataaggtt tgattgtgct agctacgtcc tgtgcagcag ttaattgtca ggtcatacgt

3480

cataattttt agcatgtctg ttttgtttg atttcgttgt ctgattaggc tgtagatagt

3540

ttcgatctac ctgtcggttt attttattaa aatttggatc cgtatgtgtg tcacatatat

3600

cttcatgatt aagatggagt tatatgggta ggttatacat gtggctgtgg atcatgatta

3660

agatggattg aagtatctct ttatctttta gttaggatag attattatat atgttgctgt

3720

tgattttatt ggttctttat tatatatatt catgcttata tacataaaag caatgtgcta

3780

ttacagttta atagttcttg attatctaat aaacaaataa ggataggtat atttgttgct

3840

gttggtttta ctggtactct attagatagt actttgacat gaagcaacat cctgctatgg

3900

attaataatt attcttcgtc taataaaaag catggttttt aattattttg atttgatata

3960

cttggatgat gtcatatgca gcagctattt gtgaattttt cggccgtatc ttcatattgc

4020

ttgggactgt ttctttggtt gataactcac cctgttgttt ggtgatcctt ctgcaggtg

4079

<210> 79

<211> 2831

<212> DNA

<213> Schizachyium scoparium

<400> 79

ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag

60

agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga

120

gtgaattgat aaatttattc taggtgacat ggccttaacg atggccagta cgtggttaaa

180

tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca

240

ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt

300

tcttgtaaat tacgcttttc tcaaaaaaat tggatcatgc atttatttca ctctaggtgt

360

gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct

420

gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc

480

atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta

540

catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt

600

ttttgagtga aataataaaa ttttaaagca atgttacaag agaagctctg gagataaaag

660

ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat

720

tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt

780

acacgacgct ggcgcttatg tggacgttaa tcggtaattc ttcattttat tttattttgt

840

tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc

900

cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat

960

tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa

1020

catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa

1080

catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca

1140

aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac

1200

acgctcatgc catccatatc attcaacttt tgaatcgttt actatacaat attatttcta

1260

aatttggctg taaagatggc attgatttca taaatatgaa aaataccaaa ttgcacattt

1320

tctttctata ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca

1380

tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga

1440

agtaattttc aatactaaga aacaacaaaa ttttggttttt ttttgtttgg tttcgattat

1500

agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca

1560

tttaatattg taactgattc aagctgagta atagatgaga tgagtgtgtt aggatgtgta

1620

gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta

1680

tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac

1740

atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg

1800

cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt

1860

tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat

1920

tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt

1980

ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt

2040

aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa

2100

gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg

2160

atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag

2220

ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa

2280

cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg

2340

aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc

2400

ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag

2460

atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat

2520

taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt

2580

atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga

2640

caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt

2700

ccgcttcctc ctccgacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt

2760

tcccaccgct ccttcctttt cccttcatcg cccgcagcta taaatagcca cccccgtccg

2820

caacttcttt c

2831

<210> 80

<211> 95

<212> DNA

<213> Schizachyium scoparium

<400> 80

cccaacctca tcttttgttc ggagcacgca cacaatccga tcgatcccca atcccctcgt

60

ctctcctcgc gagcctcgtc gatccgccat tcaag

95

<210> 81

<211> 1153

<212> DNA

<213> Schizachyium scoparium

<400> 81

gtacggcgat catcctccct ccctctctac ctgctcttct gtagatcggc gaccccatcc

60

atggttaggg cctgctagtt ctgttcctgt ttttttttcca tggctgcgag gtagaataga

120

tctgatggcg ttatgatggt taatttgtca tactcttgcg gtctatggggt ccctttaggt

180

catcaattta attttgggtg gttgagatcg gtgatccatg gttagtaccc tagtcagtgg

240

ggttggatcc gtgctattag ggttcgtaga tggattctga tggctcagta actgggaatc

300

ctaggatggt tccatctggt ttgcagatga gaacgatttc atcatctgct atatcttgtt

360

tcgttgcgta ggttctgttt aaactaatcc gtggtatgat gttagccttt gataaggttg

420

atttcatcat ctgctatatc ttgtttcgtt gcgtaggttc tgtttaaact aatccgtggt

480

atgatgttag cctttgataa ggtttgattg tgctagctac gtcctgtgca gcagttaatt

540

gtcaggtcat acgtcataat ttttagcatg tctgtttttg tttgatttcg ttgtctgatt

600

aggctgtaga tagtttcgat ctacctgtcg gtttatttta ttaaaatttg gatccgtatg

660

tgtgtcacat atatcttcat gattaagatg gagttatatg ggtaggttat acatgtggct

720

gtggatcatg attaagatgg attgaagtat ctctttatct tttagttagg atagattatt

780

atatatgttg ctgttgattt tattggttct ttattatata tattcatgct tatatacata

840

aaagcaatgt gctattacag tttaatagtt cttgattatc taataaacaa ataaggatag

900

gtatatttgt tgctgttggt tttactggta ctctattaga tagtactttg acatgaagca

960

acatcctgct atggattaat aattattctt cgtctaataa aaagcatggt ttttaattat

1020

tttgatttga tatacttgga tgatgtcata tgcagcagct atttgtgaat ttttcggccg

1080

tatcttcata ttgcttggga ctgtttcttt ggttgataac tcaccctgtt gtttggtgat

1140

ccttctgcag gtg

1153

<210> 82

<211> 3281

<212> DNA

<213> Schizachyium scoparium

<400> 82

gtggacgtta atcggtaatt cttcatttta tttttttttg ttgtcaccgc gtacatttgg

60

gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata

120

gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat

180

tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa

240

ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa

300

tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt

360

ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat

420

cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg

480

cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat

540

tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact

600

gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag

660

aaacaacaaa atttggtttt ttttgtttg gtttcgatta tagccgaact aaccaaattt

720

aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt

780

caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat

840

tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag

900

tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc

960

agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt

1020

tatatagtga atacagttta ctcttagtag tatatgtaag ttcagccttt tctattgtag

1080

gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc

1140

ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga

1200

gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt

1260

catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa

1320

atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat

1380

aattttagtt tacctttttt tgtttgattt taattttaga agggtttata gcaggactta

1440

aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa

1500

cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca

1560

cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc

1620

tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga

1680

aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt

1740

ctcgtaaagt acattcaaac tgtgtaatta gttatttttat ttatctacat ttaatactctct

1800

atgaatgcgt caagagattt gatgtgactt taatgtgacg gacaatctga aacttttacg

1860

caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga

1920

cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt

1980

ttcccttcat cgcccgcagc tataaatagc cacccccgtc cgcaacttct ttccccaacc

2040

tcatcttttg ttcggagcac gcacacaatc cgatcgatcc ccaatcccct cgtctctcct

2100

cgcgagcctc gtcgatccgc cattcaaggt acggcgatca tcctccctcc ctctctacct

2160

gctcttctgt agatcggcga ccccatccat ggttagggcc tgctagttct gttcctgttt

2220

tttttccatg gctgcgaggt agaatagatc tgatggcgtt atgatggtta atttgtcata

2280

ctcttgcggt ctatgggtcc ctttaggtca tcaatttaat tttgggtggt tgagatcggt

2340

gatccatggt tagtacccta gtcagtgggg ttggatccgt gctattaggg ttcgtagatg

2400

gattctgatg gctcagtaac tgggaatcct aggatggttc catctggttt gcagatgaga

2460

acgatttcat catctgctat atcttgtttc gttgcgtagg ttctgtttaa actaatccgt

2520

ggtatgatgt tagcctttga taaggttgat ttcatcatct gctatatctt gtttcgttgc

2580

gtaggttctg tttaaactaa tccgtggtat gatgttagcc tttgataagg tttgattgtg

2640

ctagctacgt cctgtgcagc agttaattgt caggtcatac gtcataattt ttagcatgtc

2700

tgtttttgtt tgatttcgtt gtctgattag gctgtagata gtttcgatct acctgtcggt

2760

ttattttatt aaaatttgga tccgtatgtg tgtcacatat atcttcatga ttaagatgga

2820

gttatatggg taggttatac atgtggctgt ggatcatgat taagatggat tgaagtatct

2880

ctttatcttt tagttaggat agattattat atatgttgct gttgatttta ttggttcttt

2940

attatatata ttcatgctta tatacataaa agcaatgtgc tattacagtt taatagttct

3000

tgattatcta ataaacaaat aaggataggt atatttgttg ctgttggttt tactggtact

3060

ctattagata gtactttgac atgaagcaac atcctgctat ggattaataa ttattcttcg

3120

tctaataaaa agcatggttt ttaattattt tgatttgata tacttggatg atgtcatatg

3180

cagcagctat ttgtgaattt ttcggccgta tcttcatatt gcttgggact gtttctttgg

3240

ttgataactc accctgttgt ttggtgatcc ttctgcaggt g

3281

<210> 83

<211> 2033

<212> DNA

<213> Schizachyium scoparium

<400> 83

gtggacgtta atcggtaatt cttcatttta tttttttttg ttgtcaccgc gtacatttgg

60

gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata

120

gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat

180

tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa

240

ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa

300

tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt

360

ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat

420

cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg

480

cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat

540

tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact

600

gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag

660

aaacaacaaa atttggtttt ttttgtttg gtttcgatta tagccgaact aaccaaattt

720

aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt

780

caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat

840

tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag

900

tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc

960

agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt

1020

tatatagtga atacagttta ctcttagtag tatatgtaag ttcagccttt tctattgtag

1080

gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc

1140

ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga

1200

gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt

1260

catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa

1320

atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat

1380

aattttagtt tacctttttt tgtttgattt taattttaga agggtttata gcaggactta

1440

aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa

1500

cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca

1560

cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc

1620

tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga

1680

aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt

1740

ctcgtaaagt acattcaaac tgtgtaatta gttatttttat ttatctacat ttaatactctct

1800

atgaatgcgt caagagattt gatgtgactt taatgtgacg gacaatctga aacttttacg

1860

caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga

1920

cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt

1980

ttcccttcat cgcccgcagc tataaaatagc cacccccgtc cgcaacttct ttc

2033

<210> 84

<211> 2294

<212> DNA

<213> Schizachyium scoparium

<400> 84

gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag

60

tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac

120

aattgtttca ttattcgcgt tcgaagcagc ttcttcgtag attttgcgag ggaaggctgc

180

ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt

240

tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt

300

ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat

360

gttttggtgg ttttgatttc tagcctccac aataatttta gtttacctttt ttttgtttga

420

ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag

480

taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg

540

ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca

600

tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc

660

caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc

720

caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa

780

ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga

840

ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt

900

ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca

960

gcacggggat tcctttccca ccgctccttc ctttttccctt catcgcccgc agctataaat

1020

agccaccccc gtccgcaact tctttcccca acctcatctt ttgttcggag cacgcacaca

1080

atccgatcga tccccaatcc cctcgtctct cctcgcgagc ctcgtcgatc cgccattcaa

1140

ggtacggcga tcatcctccc tccctctcta cctgctcttc tgtagatcgg cgaccccatc

1200

catggttagg gcctgctagt tctgttcctg ttttttttcc atggctgcga ggtagaatag

1260

atctgatggc gttatgatgg ttaatttgtc atactcttgc ggtctatgggg tccctttaggg

1320

tcatcaattt aattttgggt ggttgagatc ggtgatccat ggttagtacc ctagtcagtg

1380

gggttggatc cgtgctatta gggttcgtag atggattctg atggctcagt aactgggaat

1440

cctaggatgg ttccatctgg tttgcagatg agaacgattt catcatctgc tatatcttgt

1500

ttcgttgcgt aggttctgtt taaactaatc cgtggtatga tgttagcctt tgataaggtt

1560

gatttcatca tctgctatat cttgtttcgt tgcgtaggtt ctgtttaaac taatccgtgg

1620

tatgatgtta gcctttgata aggtttgatt gtgctagcta cgtcctgtgc agcagttaat

1680

tgtcaggtca tacgtcataa tttttagcat gtctgttttt gtttgatttc gttgtctgat

1740

taggctgtag atagtttcga tctacctgtc ggtttatttt attaaaattt ggatccgtat

1800

gtgtgtcaca tatatcttca tgattaagat ggagttatat gggtaggtta tacatgtggc

1860

tgtggatcat gattaagatg gattgaagta tctctttatc ttttagttag gatagattat

1920

tatatatgtt gctgttgatt ttattggttc ttttattatat atattcatgc ttatatacat

1980

aaaagcaatg tgctattaca gtttaatagt tcttgattat ctaataaaca aataaggata

2040

ggtatatttg ttgctgttgg ttttactggt actctattag atagtacttt gacatgaagc

2100

aacatcctgc tatggattaa taattattct tcgtctaata aaaagcatgg tttttaatta

2160

ttttgatttg atatacttgg atgatgtcat atgcagcagc tatttgtgaa tttttcggcc

2220

gtatcttcat attgcttggg actgtttctt tggttgataa ctcaccctgt tgtttggtga

2280

tccttctgca ggtg

2294

<210> 85

<211> 1046

<212> DNA

<213> Schizachyium scoparium

<400> 85

gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag

60

tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac

120

aattgtttca ttattcgcgt tcgaagcagc ttcttcgtag attttgcgag ggaaggctgc

180

ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt

240

tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt

300

ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat

360

gttttggtgg ttttgatttc tagcctccac aataatttta gtttacctttt ttttgtttga

420

ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag

480

taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg

540

ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca

600

tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc

660

caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc

720

caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa

780

ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga

840

ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt

900

ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca

960

gcacggggat tcctttccca ccgctccttc ctttttccctt catcgcccgc agctataaat

1020

agccaccccc gtccgcaact tctttc

1046

<210> 86

<211> 1795

<212> DNA

<213> Schizachyium scoparium

<400> 86

gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg

60

tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct

120

ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc

180

tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg

240

ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct

300

acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat

360

ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc

420

ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc

480

accgctcctt ccttttccct tcatcgccg cagctataaa tagccacccc cgtccgcaac

540

ttctttcccc aacctcatct tttgttcgga gcacgcacac aatccgatcg atccccaatc

600

ccctcgtctc tcctcgcgag cctcgtcgat ccgccattca aggtacggcg atcatcctcc

660

ctccctctct acctgctctt ctgtagatcg gcgaccccat ccatggttag ggcctgctag

720

ttctgttcct gttttttttc catggctgcg aggtagaata gatctgatgg cgttatgatg

780

gttaatttgt catactcttg cggtctatgg gtccctttag gtcatcaatt taattttggg

840

tggttgagat cggtgatcca tggttagtac cctagtcagt ggggttggat ccgtgctatt

900

agggttcgta gatggattct gatggctcag taactgggaa tcctaggatg gttccatctg

960

gtttgcagat gagaacgatt tcatcatctg ctatatcttg tttcgttgcg taggttctgt

1020

ttaaactaat ccgtggtatg atgttagcct ttgataaggt tgatttcatc atctgctata

1080

tcttgtttcg ttgcgtaggt tctgtttaaa ctaatccgtg gtatgatgtt agcctttgat

1140

aaggtttgat tgtgctagct acgtcctgtg cagcagttaa ttgtcaggtc atacgtcata

1200

atttttagca tgtctgtttt tgtttgattt cgttgtctga ttaggctgta gatagtttcg

1260

atctacctgt cggtttattt tattaaaatt tggatccgta tgtgtgtcac atatatcttc

1320

atgattaaga tggagttata tgggtaggtt atacatgtgg ctgtggatca tgattaagat

1380

ggattgaagt atctctttat cttttagtta ggatagatta ttatatatgt tgctgttgat

1440

tttattggtt ctttattata tatattcatg cttatataca taaaagcaat gtgctattac

1500

agtttaatag ttcttgatta tctaataaac aaataaggat aggtatattt gttgctgttg

1560

gttttactgg tactctatta gatagtactt tgacatgaag caacatcctg ctatggatta

1620

ataattattc ttcgtctaat aaaaagcatg gtttttaatt attttgattt gatatacttg

1680

gatgatgtca tatgcagcag ctatttgtga atttttcggc cgtatcttca tattgcttgg

1740

gactgtttct ttggttgata actcaccctg ttgtttggtg atccttctgc aggtg

1795

<210> 87

<211> 547

<212> DNA

<213> Schizachyium scoparium

<400> 87

gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg

60

tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct

120

ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc

180

tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg

240

ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct

300

acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat

360

ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc

420

ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc

480

accgctcctt ccttttccct tcatcgccg cagctataaa tagccacccc cgtccgcaac

540

ttctttc

547

<210> 88

<211> 3357

<212> DNA

<213> Sorghastrum nutans

<400> 88

gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt

60

gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc

120

cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt

180

tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt

240

gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg

300

taatgtagtc cggcacggca agttagactc tgtgtgtagt gttattag ccggcctctt

360

taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt

420

caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa

480

agttatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat

540

agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt

600

gagctttggc tcataatcta aataattatg agagagtggg atcgaccaca tattcatttt

660

gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg

720

ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata

780

tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat

840

atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt

900

gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcgggg ctttaagcca

960

aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata

1020

cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga

1080

gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat

1140

caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc

1200

aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag

1260

tcgttttgat tttattggta catacatttt gctatgtgtt tagatataat aatatgtcta

1320

gatacattgg atgaaccgaa aaaatcgaaa cgacttataa tttggatcga aaggagtatt

1380

tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact

1440

atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta

1500

caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg

1560

agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa

1620

tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa

1680

ctatattttc atagtatact tatttaatgt tataaattttt tataattttt tttataattt

1740

tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc

1800

aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa

1860

ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc

1920

tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt

1980

ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc

2040

gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac

2100

acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct

2160

ctccttcggt cctccctcca tcataaatag ccacccccct cccaccttct ttccccacct

2220

cgtctcccct cgtgttattc ggagcacaga cacaccccga tccccaatcc tctcctcgcg

2280

agcctcgtcg atccccgctt caaggtacgg cgatcatcct ccctccctaa ctccaatccg

2340

tggttagggc ctgctagatc gtcctccctc cctacctgcg atccgtggtt cgcgcctgct

2400

agttctgttt cctgtttgtc gatggctgcg aggtataata gatctgatgg cgtgcggtgt

2460

gacggttaaa ttcacatgct cttgcgattt atacgcgaat cgatctggga ttgctcgaga

2520

tcggtgatcc atggttagaa ccctaggcgg tggagtcggg ttaaatccgt gctgttaggg

2580

ttcgtaggtg gatgcgacct gttctggttg tttacttgtc agtatttagg aatcctacta

2640

ggatggttct agctggttcg cagatgagat cgatttcatg atctgctata tctttcgttg

2700

cctaagtttc gtttaatctg tccgtggtat gatgttagcc tttgatatgc ttcgatcgtg

2760

ctagctacct cctgtgcact aaattatcag ctcgtaattt ttagcatgcc cttttttttt

2820

tgggtattgt tcgattgagg tgtcgttcta gatcagagta ggaagactgt ttcaaactac

2880

ctgctggatt tattaaattt ggatctgtat gagtatcaca tatatctcca taatttagat

2940

ggatggaaat atcccttttt cttttagata ctgtttggta tagattttgc tgtgggtttt

3000

actggtactt agatactctt cgtttagata tggatatgtt tacatgcaga tacatgaagc

3060

aacatgctgc tacagtttaa tatggatagg tgtatatgtt gttgtgggtc ctttacttac

3120

atgcttagat acatgaagca acatgctgct acgtttaata attattgttt atctgatctg

3180

atttaaacaa acatgctttt taattgtcct gaaatgcttg gatgatggca tatgcagcag

3240

ctatgtgtgg attttaaata cccagcatga gcatgcatga ccctaactta gtatgctgtt

3300

tatttgcttg acttttcttt tgttgatact cacccttttg tttgttgact cttgcag

3357

<210> 89

<211> 2218

<212> DNA

<213> Sorghastrum nutans

<400> 89

gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt

60

gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc

120

cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt

180

tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt

240

gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg

300

taatgtagtc cggcacggca agttagactc tgtgtgtagt gttattag ccggcctctt

360

taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt

420

caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa

480

agttatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat

540

agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt

600

gagctttggc tcataatcta aataattatg agagagtggg atcgaccaca tattcatttt

660

gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg

720

ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata

780

tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat

840

atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt

900

gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcgggg ctttaagcca

960

aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata

1020

cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga

1080

gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat

1140

caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc

1200

aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag

1260

tcgttttgat tttattggta catacatttt gctatgtgtt tagatataat aatatgtcta

1320

gatacattgg atgaaccgaa aaaatcgaaa cgacttataa tttggatcga aaggagtatt

1380

tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact

1440

atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta

1500

caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg

1560

agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa

1620

tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa

1680

ctatattttc atagtatact tatttaatgt tataaattttt tataattttt tttataattt

1740

tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc

1800

aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa

1860

ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc

1920

tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt

1980

ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc

2040

gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac

2100

acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct

2160

ctccttcggt cctccctcca tcataaatag ccacccccct cccaccttct ttccccac

2218

<210> 90

<211> 86

<212> DNA

<213> Sorghastrum nutans

<400> 90

ctcgtctccc ctcgtgttat tcggagcaca gacacacccc gatccccaat cctctcctcg

60

cgagcctcgt cgatccccgc ttcaag

86

<210> 91

<211> 1053

<212> DNA

<213> Sorghastrum nutans

<400> 91

gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc

60

tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg tttgtcgatg

120

gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg

180

cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct

240

aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc

300

tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct ggttcgcaga

360

tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg

420

tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat

480

tatcagctcg taatttttag catgcccttt ttttttttggg tattgttcga ttgaggtgtc

540

gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat

600

ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt

660

tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt

720

tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg

780

gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat

840

gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat

900

tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca

960

gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt

1020

gatactcacc cttttgtttg ttgactcttg cag

1053

<210> 92

<211> 3106

<212> DNA

<213> Sorghastrum nutans

<400> 92

agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc

60

acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa

120

tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg

180

atataatata caagacaag tatacaataa agtgacatta gcgtaaagtt atatgacata

240

tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta

300

tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat

360

aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct

420

ctccgtttt tataagttgc tttgattttt ttttatatca attttgctat acatctaaac

480

ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag

540

atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc

600

ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg

660

caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc

720

tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag

780

cgagtgtgtg agtaataatt tttctctagt gtgtacgagt atgcgagtaa taattttaaa

840

tctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct

900

aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt

960

ctcaaatgat tagatcctat aatactccta cgtactaaat tataagtcgt tttgatttta

1020

ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga

1080

accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt

1140

tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa

1200

tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca

1260

acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag

1320

tcattccaag atcttagaaa attaaagtat attaagtttg attaaattta tatgacaagt

1380

aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag

1440

tatacttatt taatgttata aatttttata atttttttta taattttagc taaactcgag

1500

atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga

1560

gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag

1620

ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc

1680

catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc

1740

cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg cggatcgcgt ggcggagcgg

1800

agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg

1860

aaccgtgacg gcaccggggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc

1920

ccctccatcat aaatagccac ccccctccca ccttctttcc ccacctcgtc tcccctcgtg

1980

ttattcggag cacagacaca ccccgatccc caatcctctc ctcgcgagcc tcgtcgatcc

2040

ccgcttcaag gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc

2100

tagatcgtcc tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg

2160

tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca

2220

catgctcttg cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg

2280

ttagaaccct aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg

2340

cgacctgttc tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct

2400

ggttcgcaga tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt

2460

aatctgtccg tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg

2520

tgcactaaat tatcagctcg taatttttag catgcccttt ttttttttggg tattgttcga

2580

ttgaggtgtc gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt

2640

aaatttggat ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc

2700

ctttttcttt tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat

2760

actcttcgtt tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca

2820

gtttaatatg gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat

2880

gaagcaacat gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat

2940

gctttttaat tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt

3000

taaataccca gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt

3060

ttcttttgtt gatactcacc cttttgtttg ttgactcttg caggtg

3106

<210> 93

<211> 1964

<212> DNA

<213> Sorghastrum nutans

<400> 93

agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc

60

acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa

120

tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg

180

atataatata caagacaag tatacaataa agtgacatta gcgtaaagtt atatgacata

240

tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta

300

tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat

360

aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct

420

ctccgtttt tataagttgc tttgattttt ttttatatca attttgctat acatctaaac

480

ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag

540

atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc

600

ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg

660

caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc

720

tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag

780

cgagtgtgtg agtaataatt tttctctagt gtgtacgagt atgcgagtaa taattttaaa

840

tctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct

900

aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt

960

ctcaaatgat tagatcctat aatactccta cgtactaaat tataagtcgt tttgatttta

1020

ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga

1080

accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt

1140

tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa

1200

tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca

1260

acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag

1320

tcattccaag atcttagaaa attaaagtat attaagtttg attaaattta tatgacaagt

1380

aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag

1440

tatacttatt taatgttata aatttttata atttttttta taattttagc taaactcgag

1500

atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga

1560

gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag

1620

ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc

1680

catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc

1740

cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg cggatcgcgt ggcggagcgg

1800

agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg

1860

aaccgtgacg gcaccggggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc

1920

ccctccatcat aaatagccac ccccctccca ccttctttcc ccac

1964

<210> 94

<211> 1056

<212> DNA

<213> Sorghastrum nutans

<400> 94

gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc

60

tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg tttgtcgatg

120

gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg

180

cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct

240

aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc

300

tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct ggttcgcaga

360

tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg

420

tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat

480

tatcagctcg taatttttag catgcccttt ttttttttggg tattgttcga ttgaggtgtc

540

gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat

600

ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt

660

tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt

720

tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg

780

gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat

840

gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat

900

tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca

960

gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt

1020

gatactcacc cttttgtttg ttgactcttg caggtg

1056

<210> 95

<211> 2165

<212> DNA

<213> Sorghastrum nutans

<400> 95

gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt

60

ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat

120

gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga

180

gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa

240

atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg

300

tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg

360

gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga

420

ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc

480

atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat

540

aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca

600

tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca

660

gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg

720

acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg

780

gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc

840

ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct

900

ctcacacggc acggaacgga accgtgacgg caccgggcag cacgggcggg attccttccc

960

cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc

1020

cacctcgtct cccctcgtgt tattcggagc acagacacac cccgatcccc aatcctctcc

1080

tcgcgagcct cgtcgatccc cgcttcaagg tacggcgatc atcctccctc cctaactcca

1140

atccgtggtt agggcctgct agatcgtcct ccctccctac ctgcgatccg tggttcgcgc

1200

ctgctagttc tgtttcctgt ttgtcgatgg ctgcgaggta taatagatct gatggcgtgc

1260

ggtgtgacgg ttaaattcac atgctcttgc gatttatacg cgaatcgatc tgggattgct

1320

cgagatcggt gatccatggt tagaacccta ggcggtggag tcgggttaaa tccgtgctgt

1380

tagggttcgt aggtggatgc gacctgttct ggttgtttac ttgtcagtat ttaggaatcc

1440

tactaggatg gttctagctg gttcgcagat gagatcgatt tcatgatctg ctatatcttt

1500

cgttgcctaa gtttcgttta atctgtccgt ggtatgatgt tagcctttga tatgcttcga

1560

tcgtgctagc tacctcctgt gcactaaatt atcagctcgt aatttttagc atgccctttt

1620

ttttttgggt attgttcgat tgaggtgtcg ttctagatca gagtaggaag actgtttcaa

1680

actacctgct ggatttatta aatttggatc tgtatgagta tcacatatat ctccataatt

1740

tagatggatg gaaatatccc tttttctttt agatactgtt tggtatagat tttgctgtgg

1800

gttttactgg tacttagata ctcttcgttt agatatggat atgtttacat gcagatacat

1860

gaagcaacat gctgctacag tttaatatgg ataggtgtat atgttgttgt gggtccttta

1920

cttacatgct tagatacatg aagcaacatg ctgctacgtt taataattat tgtttatctg

1980

atctgattta aacaaacatg ctttttaatt gtcctgaaat gcttggatga tggcatatgc

2040

agcagctatg tgtggatttt aaatacccag catgagcatg catgacccta acttagtatg

2100

ctgtttattt gcttgacttt tcttttgttg atactcaccc ttttgtttgt tgactcttgc

2160

aggtg

2165

<210> 96

<211> 1023

<212> DNA

<213> Sorghastrum nutans

<400> 96

gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt

60

ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat

120

gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga

180

gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa

240

atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg

300

tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg

360

gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga

420

ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc

480

atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat

540

aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca

600

tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca

660

gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg

720

acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg

780

gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc

840

ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct

900

ctcacacggc acggaacgga accgtgacgg caccgggcag cacgggcggg attccttccc

960

cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc

1020

cac

1023

<210> 97

<211> 1866

<212> DNA

<213> Sorghastrum nutans

<400> 97

gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac

60

ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg

120

attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt

180

catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta

240

taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac

300

atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc

360

agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt

420

gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc

480

ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg

540

cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc

600

tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacggggcgg gattccttcc

660

ccacctctcc ttcggtcctc ccctccatcat aaatagccac ccccctccca ccttctttcc

720

ccacctcgtc tcccctcgtg ttattcggag cacagacaca ccccgatccc caatcctctc

780

ctcgcgagcc tcgtcgatcc ccgcttcaag gtacggcgat catcctccct ccctaactcc

840

aatccgtggt tagggcctgc tagatcgtcc tccctcccta cctgcgatcc gtggttcgcg

900

cctgctagtt ctgtttcctg tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg

960

cggtgtgacg gttaaattca catgctcttg cgatttatac gcgaatcgat ctgggattgc

1020

tcgagatcgg tgatccatgg ttagaaccct aggcggtgga gtcgggttaa atccgtgctg

1080

ttagggttcg taggtggatg cgacctgttc tggttgttta cttgtcagta tttaggaatc

1140

ctactaggat ggttctagct ggttcgcaga tgagatcgat ttcatgatct gctatatctt

1200

tcgttgccta agtttcgttt aatctgtccg tggtatgatg ttagcctttg atatgcttcg

1260

atcgtgctag ctacctcctg tgcactaaat tatcagctcg taatttttag catgcccttt

1320

ttttttggg tattgttcga ttgaggtgtc gttctagatc agagtaggaa gactgtttca

1380

aactacctgc tggatttatt aaatttggat ctgtatgagt atcacatata tctccataat

1440

ttagatggat ggaaatatcc ctttttcttt tagatactgt ttggtataga ttttgctgtg

1500

ggttttactg gtacttagat actcttcgtt tagatatgga tatgtttaca tgcagataca

1560

tgaagcaaca tgctgctaca gtttaatatg gataggtgta tatgttgttg tgggtccttt

1620

acttacatgc ttagatacat gaagcaacat gctgctacgt ttaataatta ttgtttatct

1680

gatctgattt aaacaaacat gctttttaat tgtcctgaaa tgcttggatg atggcatatg

1740

cagcagctat gtgtggattt taaataccca gcatgagcat gcatgaccct aacttagtat

1800

gctgtttatt tgcttgactt ttcttttgtt gatactcacc cttttgtttg ttgactcttg

1860

caggtg

1866

<210> 98

<211> 724

<212> DNA

<213> Sorghastrum nutans

<400> 98

gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac

60

ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg

120

attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt

180

catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta

240

taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac

300

atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc

360

agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt

420

gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc

480

ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg

540

cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc

600

tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacggggcgg gattccttcc

660

ccacctctcc ttcggtcctc ccctccatcat aaatagccac ccccctccca ccttctttcc

720

ccc

724

<210> 99

<211> 2625

<212> DNA

<213> Setaria italica

<400> 99

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg

1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg

1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt

1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctgggg

1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg

1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg

1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag

1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg

1500

caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg

1560

tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg

1620

tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt

1680

gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc

1740

agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa

1800

tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac

1860

ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc

1920

ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc

1980

aatatatttt gcatgctttt ggcctgttat tcttgccaac aagttgtcct ggtaaaaagt

2040

agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct

2100

gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg

2160

aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta

2220

gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct

2280

agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc

2340

gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg

2400

aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac

2460

atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt

2520

tctctattga gtttatggac ttttgtgtgt gaagttatat ttcattttgc tcaaaactca

2580

tgtttgcaag ctttctgaca ttattctatt gttctgaaac agggt

2625

<210> 100

<211> 1492

<212> DNA

<213> Setaria italica

<400> 100

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg

1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg

1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt

1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctgggg

1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg

1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg

1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag

1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc

1492

<210> 101

<211> 127

<212> DNA

<213> Setaria italica

<400> 101

cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt

60

ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc

120

cagcaag

127

<210> 102

<211> 1006

<212> DNA

<213> Setaria italica

<400> 102

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

120

cagttatttg caatttgcga tttgctcgtt tgttgcgcag cgtagtttat gtttggagta

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

240

cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt

300

cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc

360

caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag

420

tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc

480

tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg

540

gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt

600

agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc

660

tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat

720

cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag

780

gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa

840

catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg

900

ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc

960

atgtttgcaa gctttctgac attattctat tgttctgaaa cagggt

1006

<210> 103

<211> 2625

<212> DNA

<213> Setaria italica

<400> 103

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg

1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg

1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt

1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctgggg

1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg

1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg

1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag

1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg

1500

caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg

1560

tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg

1620

tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt

1680

gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc

1740

agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa

1800

tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac

1860

ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc

1920

ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc

1980

aatatatttt gcatgctttt ggcctgttat tcttgccaac aagttgtcct ggtaaaaagt

2040

agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct

2100

gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg

2160

aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta

2220

gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct

2280

agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc

2340

gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg

2400

aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac

2460

atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt

2520

tctctattga gtttatggac ttttgtgtgt gaagttatat ttcattttgc tcaaaactca

2580

tgtttgcaag ctttctgaca ttattctatt gttctgaaac aggtg

2625

<210> 104

<211> 1492

<212> DNA

<213> Setaria italica

<400> 104

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg

1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg

1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt

1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctgggg

1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg

1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg

1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag

1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc

1492

<210> 105

<211> 1006

<212> DNA

<213> Setaria italica

<400> 105

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

120

cagttatttg caatttgcga tttgctcgtt tgttgcgcag cgtagtttat gtttggagta

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

240

cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt

300

cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc

360

caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag

420

tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc

480

tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg

540

gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt

600

agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc

660

tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat

720

cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag

780

gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa

840

catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg

900

ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc

960

atgtttgcaa gctttctgac attattctat tgttctgaaa caggtg

1006

<210> 106

<211> 2167

<212> DNA

<213> Setaria italica

<400> 106

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg

60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt

120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg

180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca

240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg

300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacgggg

360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt

420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac

480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca

540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag

600

agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg

660

gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc

720

ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gccggggtag

780

aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg

840

acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc

900

acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg

960

gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa

1020

ataccctccc atcccgttgc cgcaagactc agatcagatt ccgatcccca gttcttcccc

1080

aatcaccttg tggtctctcg tgtcgcggtt cccagggacg cctccggctc gtcgctcgac

1140

agcgatctcc gccccagcaa ggtatagatt cagttccttg ctccgatccc aatctggttg

1200

agatgttgct ccgatgcgac ttgattatgt catatatctg cggtttgcac cgatctgaag

1260

cctagggttt ctcgagcgac ccagttattt gcaatttgcg atttgctcgt ttgttgcgca

1320

gcgtagttta tgtttggagt aatcgaggat ttgtatgcgg cgtcggcgct acctgcttaa

1380

tcacgccatg tgacgcggtt acttgcagag gctgggttct gttatgtcgt gatctaagaa

1440

tctagattag gctcagtcgt tcttgctgtc gactagtttg ttttgatatc catgtagtac

1500

aagttactta aaatttaggt ccaatatatt ttgcatgctt ttggcctgtt attcttgcca

1560

acaagttgtc ctggtaaaaa gtagatgtga aagtcacgta ttgggacaaa ttgatggttt

1620

agtgctatag ttctatagtt ctgtgataca tctatctgat ttttttttggt ctattggtgc

1680

ctaacttatc tgaaaatcat ggaacatgag gctagtttga tcatggttta gttcattgtg

1740

attaataatg tatgatttag tagctatttt ggtgatcgtg tcattttatt tgtgaatgga

1800

atcattgtat gtaaatgaag ctagttcagg ggttacgatg tagctggctt tgtattctaa

1860

aggctgctat tattcatcca tcgatttcac ctatatgtaa tccagagctt ttgatgtgaa

1920

atttgtctga tccttcacta ggaaggacag aacattgtta atattttggc acatctgtct

1980

tattctcatc ctttgtttga acatgttagc ctgttcaaac agatactgtt gtaatgtcct

2040

agttatatag gtacatatgt gttctctatt gagtttatgg acttttgtgt gtgaagttat

2100

atttcatttt gctcaaaact catgtttgca agctttctga cattattcta ttgttctgaa

2160

acaggtg

2167

<210> 107

<211> 1034

<212> DNA

<213> Setaria italica

<400> 107

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg

60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt

120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg

180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca

240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg

300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacgggg

360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt

420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac

480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca

540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag

600

agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg

660

gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc

720

ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gccggggtag

780

aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg

840

acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc

900

acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg

960

gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa

1020

ataccctccc atcc

1034

<210> 108

<211> 1813

<212> DNA

<213> Setaria italica

<400> 108

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc

300

gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactggggc

360

caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtagggggccc

420

gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct

480

aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg

540

aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc

600

cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag

660

gcataaatac cctcccatcc cgttgccgca agactcagat cagattccga tccccagttc

720

ttccccaatc accttgtggt ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg

780

ctcgacagcg atctccgccc cagcaaggta tagattcagt tccttgctcc gatcccaatc

840

tggttgagat gttgctccga tgcgacttga ttatgtcata tatctgcggt ttgcaccgat

900

ctgaagccta gggtttctcg agcgacccag ttatttgcaa tttgcgattt gctcgtttgt

960

tgcgcagcgt agtttatgtt tggagtaatc gaggatttgt atgcggcgtc ggcgctacct

1020

gcttaatcac gccatgtgac gcggttactt gcagaggctg ggttctgtta tgtcgtgatc

1080

taagaatcta gattaggctc agtcgttctt gctgtcgact agtttgtttt gatatccatg

1140

tagtacaagt tacttaaaat ttaggtccaa tatattttgc atgcttttgg cctgttattc

1200

ttgccaacaa gttgtcctgg taaaaagtag atgtgaaagt cacgtattgg gacaaattga

1260

tggtttagtg ctatagttct atagttctgt gatacatcta tctgattttt tttggtctat

1320

tggtgcctaa cttatctgaa aatcatggaa catgaggcta gtttgatcat ggtttagttc

1380

attgtgatta ataatgtatg atttagtagc tattttggtg atcgtgtcat tttatttgtg

1440

aatggaatca ttgtatgtaa atgaagctag ttcaggggtt acgatgtagc tggctttgta

1500

ttctaaaggc tgctattatt catccatcga tttcacctat atgtaatcca gagcttttga

1560

tgtgaaattt gtctgatcct tcactaggaa ggacagaaca ttgttaatat tttggcacat

1620

ctgtcttatt ctcatccttt gtttgaacat gttagcctgt tcaaacagat actgttgtaa

1680

tgtcctagtt atataggtac atatgtgttc tctattgagt ttatggactt ttgtgtgtga

1740

agttatattt cattttgctc aaaactcatg tttgcaagct ttctgacatt attctattgt

1800

tctgaaacag gtg

1813

<210> 109

<211> 680

<212> DNA

<213> Setaria italica

<400> 109

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc

300

gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactggggc

360

caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtagggggccc

420

gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct

480

aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg

540

aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc

600

cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag

660

gcataaatac cctcccatcc

680

<210> 110

<211> 2634

<212> DNA

<213> Setaria viridis

<400> 110

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt

1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct

1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg

1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg

1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat

1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc

1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca

1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc

1500

gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt

1560

gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag

1620

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

1680

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

1740

cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta

1800

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

1860

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct

1920

cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa

1980

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg

2040

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc

2100

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga

2160

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat

2220

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta

2280

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat

2340

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc

2400

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt

2460

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta

2520

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct

2580

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg

2634

<210> 111

<211> 1493

<212> DNA

<213> Setaria viridis

<400> 111

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt

1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct

1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg

1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg

1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat

1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc

1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca

1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcc

1493

<210> 112

<211> 127

<212> DNA

<213> Setaria viridis

<400> 112

cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt

60

ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc

120

cagcaag

127

<210> 113

<211> 1014

<212> DNA

<213> Setaria viridis

<400> 113

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

120

cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

240

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct

300

cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa

360

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg

420

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc

480

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga

540

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat

600

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta

660

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat

720

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc

780

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt

840

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta

900

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct

960

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg

1014

<210> 114

<211> 2634

<212> DNA

<213> Setaria viridis

<400> 114

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc

60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg

120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc

180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac

240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca

300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg

360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa

420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga

480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt

540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc

600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc

660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag

720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc

780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc

840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg

900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga

960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta

1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt

1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct

1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg

1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg

1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat

1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc

1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca

1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc

1500

gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt

1560

gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag

1620

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

1680

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

1740

cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta

1800

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

1860

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct

1920

cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa

1980

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg

2040

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc

2100

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga

2160

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat

2220

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta

2280

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat

2340

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc

2400

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt

2460

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta

2520

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct

2580

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt

2634

<210> 115

<211> 1014

<212> DNA

<213> Setaria viridis

<400> 115

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact

60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc

120

cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta

240

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct

300

cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa

360

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg

420

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc

480

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga

540

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat

600

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta

660

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat

720

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc

780

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt

840

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta

900

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct

960

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt

1014

<210> 116

<211> 2176

<212> DNA

<213> Setaria viridis

<400> 116

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg

60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt

120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg

180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca

240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg

300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacgggg

360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt

420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac

480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca

540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag

600

agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt

660

ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg

720

cctcgtcgca actcgcaacc cgttggcggga agaaaggaat ggctcgtagg ggcccgggta

780

gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac

840

gacggcccgg tcaccgggcg atggaaagag accggatcct cctcgtgaat tctggaaggc

900

cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg

960

ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata

1020

aataccctcc catcccgttg ccgcaagact cagatcagat tccgatcccc agttcttccc

1080

caatcacctt gtggtctctc gtgtcgcggt tcccagggac gcctccggct cgtcgctcga

1140

cagcgatctc cgccccagca aggtatagat tcagttcctt gctccgatcc caatctggtt

1200

gagatgttgc tccgatgcga cttgattatg tcatatatct gcggtttgca ccgatctgaa

1260

gcctagggtt tctcgagcga cccagttgtt tgcaatttgc gatttgctcg tttgttgcgc

1320

atcgtagttt atgtttggag taatcgagga tttgtatgcg gcgtcggcgc tacctgctta

1380

atcacgccat gtgacgcggt tacttgcaga ggctgggtta gtgggttctg ttatgtcgtg

1440

atctaagaat ctagattagg ctcagtcgtt cttgctgtcg actagtttgt tttgatatcc

1500

atgtagtaca agttacttaa aatttaggtc caatatattt tgcatgcttt tggcctgtta

1560

ttcttgccaa caagttgtcc tggtaaaaag tagatgtgaa agtcacgtat tgggacaaat

1620

tgtggttaa gtgctatagt tctatagttc tgtgatacat ctatctgatt ttttttggtc

1680

tattggtgcc taacttatct gaaaatcatg gaacatgagg ctagtttgat catggtttag

1740

ttcattgtga ttaataatgt atgatttagt agctattttg gtgatcgtgt cattttattt

1800

gtgaatggaa tcattgtatg taaatgaagc tagttcaggg gttatgatgt agctggcttt

1860

gtattctaaa ggctgctatt attcatccat cgatttcacc tatatgtaat ccagagcttt

1920

cgatgtgaaa tttgtctgat ccttcactag gaaggacaga acattgttaa tattttggca

1980

catctgtctt attctcatcc tttgtttgaa catgttagcc tgttcaaaca gatactgttg

2040

taatgtccta gttatatagg tacatatgtg ttctctattg agtttatgga cttttgtgtg

2100

tgaagttata tttcattttg ctcaaaactc atgtttgcaa gctttctgac attattctat

2160

tgttctgaaa caggtg

2176

<210> 117

<211> 1035

<212> DNA

<213> Setaria viridis

<400> 117

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg

60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt

120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg

180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca

240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg

300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacgggg

360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt

420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac

480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca

540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag

600

agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt

660

ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg

720

cctcgtcgca actcgcaacc cgttggcggga agaaaggaat ggctcgtagg ggcccgggta

780

gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac

840

gacggcccgg tcaccgggcg atggaaagag accggatcct cctcgtgaat tctggaaggc

900

cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg

960

ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata

1020

aataccctcc catcc

1035

<210> 118

<211> 1822

<212> DNA

<213> Setaria viridis

<400> 118

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc

300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg

360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc

420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc

480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg

540

gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga

660

ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt

720

cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc

780

gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat

840

ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga

900

tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg

960

ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc

1020

tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat

1080

gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg

1140

atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc

1200

ctgttattct tgccaacaag ttgtcctggt aaaaagtaga tgtgaaagtc acgtattggg

1260

acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt

1320

ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg

1380

gtttagttca ttgtgattaa taatgtatga tttagtagct attttggtga tcgtgtcatt

1440

ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct

1500

ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag

1560

agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt

1620

ttggcacatc tgtcttattc tcatcctttg tttgaacatg ttagcctgtt caaacagata

1680

ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt

1740

tgtgtgtgaa gttatttc attttgctca aaactcatgt ttgcaagctt tctgacatta

1800

ttctattgtt ctgaaacagg tg

1822

<210> 119

<211> 681

<212> DNA

<213> Setaria viridis

<400> 119

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc

300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg

360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc

420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc

480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg

540

gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga

660

ggcataaata ccctcccatc c

681

<210> 120

<211> 1822

<212> DNA

<213> Setaria viridis

<400> 120

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc

300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg

360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc

420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc

480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg

540

gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga

660

ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt

720

cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc

780

gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat

840

ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga

900

tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg

960

ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc

1020

tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat

1080

gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg

1140

atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc

1200

ctgttattct tgccaacaag ttgtcctggt aaaaagtaga tgtgaaagtc acgtattggg

1260

acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt

1320

ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg

1380

gtttagttca ttgtgattaa taatgtatga tttagtagct attttggtga tcgtgtcatt

1440

ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct

1500

ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag

1560

agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt

1620

ttggcacatc tgtcttattc tcatcctttg tttgaacatg ttagcctgtt caaacagata

1680

ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt

1740

tgtgtgtgaa gttatttc attttgctca aaactcatgt ttgcaagctt tctgacatta

1800

ttctattgtt ctgaaacagg gt

1822

<210> 121

<211> 681

<212> DNA

<213> Setaria viridis

<400> 121

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac

60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt

120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa

180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt

240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc

300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg

360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc

420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc

480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg

540

gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga

660

ggcataaata ccctcccatc c

681

<210> 122

<211> 1925

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 122

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg

180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga

240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac

300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta

360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt

420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt

480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt

540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc

600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg

660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac

720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt

780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc

840

ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa

900

atccacccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct

960

ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt

1020

catgtttgtg gttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat

1080

gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat

1140

cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgaatttt ttttgtttcg

1200

ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt

1260

cgggtcatct tttcatgttt ttttggctt ggttgtgatg atgtggtctg gttgggcggt

1320

cgttctagat cggagtagaa tactgtttca aactacctgg tggatttatt aaaggatctg

1380

tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat

1440

ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt

1500

ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac

1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtc atacatcttc

1620

atagttacga gtttaagatc gatggaaata tcgatctagg ataggtatac atgttgatgt

1680

gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga

1740

gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg

1800

gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt

1860

tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc

1920

aggtc

1925

<210> 123

<211> 850

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 123

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg

180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga

240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac

300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta

360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt

420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt

480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt

540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc

600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg

660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac

720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt

780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc

840

ctctttcccc

850

<210> 124

<211> 78

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 124

aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa atccacccgt

60

cggcacctcc gcttcaag

78

<210> 125

<211> 997

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 125

gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc

60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt

120

tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat

180

tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga

240

cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc

300

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat ctttttcatgt tttttttggc

360

ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt

420

caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt

480

acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg

540

ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg

600

tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta

660

attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa

720

tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat

780

atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat

840

gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt

900

ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg

960

atgctcaccc tgttgtttgg tgatacttct gcaggtc

997

<210> 126

<211> 1925

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 126

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg

180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga

240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac

300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta

360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt

420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt

480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt

540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc

600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg

660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac

720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt

780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc

840

ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa

900

atccacccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct

960

ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt

1020

catgtttgtg gttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat

1080

gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat

1140

cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgaatttt ttttgtttcg

1200

ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt

1260

cgggtcatct tttcatgttt ttttggctt ggttgtgatg atgtggtctg gttgggcggt

1320

cgttctagat cggagtagaa tactgtttca aactacctgg tggatttatt aaaggatctg

1380

tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat

1440

ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt

1500

ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac

1560

tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtc atacatcttc

1620

atagttacga gtttaagatc gatggaaata tcgatctagg ataggtatac atgttgatgt

1680

gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga

1740

gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg

1800

gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt

1860

tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc

1920

agggt

1925

<210> 127

<211> 997

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 127

gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc

60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt

120

tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat

180

tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga

240

cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc

300

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat ctttttcatgt tttttttggc

360

ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt

420

caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt

480

acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg

540

ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg

600

tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta

660

attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa

720

tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat

780

atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat

840

gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt

900

ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg

960

atgctcaccc tgttgtttgg tgatacttct gcagggt

997

<210> 128

<211> 1974

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 128

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc

180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc

240

tcagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc

300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt

360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa

420

ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc

540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa

600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg

660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca

720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc

780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc

840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt

900

tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt

960

caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt

1020

ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg

1080

ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga

1140

ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag

1200

acgggatcga tttcatgatt ttttttgttt cgttgcatag ggttggttt gcccttttcc

1260

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt

1320

cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt

1380

tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat

1440

agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg

1500

cgggttttac tgatgcatat acagagatgc ttttgttcg cttggttgtg atgatgtggt

1560

ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg

1620

gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt

1680

aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg

1740

catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta

1800

ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat

1860

atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg

1920

tactgtttct tttgtcgatg ctcaccctgt tgtttggtga tacttctgca ggtc

1974

<210> 129

<211> 887

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 129

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc

180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc

240

tcagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc

300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt

360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa

420

ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc

540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa

600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg

660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca

720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc

780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc

840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc ttttcccc

887

<210> 130

<211> 77

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 130

aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc

60

ggcacctccg cttcaag

77

<210> 131

<211> 1010

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 131

gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat

60

ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag

120

atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc

180

taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg

240

gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta

300

tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg

360

gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa

420

actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt

480

acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg

540

ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg

600

ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt

660

atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga

720

tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata

780

tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat

840

aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc

900

agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact

960

gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcaggtc

1010

<210> 132

<211> 1974

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 132

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca

60

tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc

180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc

240

tcagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc

300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt

360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa

420

ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc

540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa

600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg

660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca

720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc

780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc

840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt

900

tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt

960

caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt

1020

ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg

1080

ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga

1140

ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag

1200

acgggatcga tttcatgatt ttttttgttt cgttgcatag ggttggttt gcccttttcc

1260

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt

1320

cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt

1380

tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat

1440

agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg

1500

cgggttttac tgatgcatat acagagatgc ttttgttcg cttggttgtg atgatgtggt

1560

ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg

1620

gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt

1680

aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg

1740

catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta

1800

ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat

1860

atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg

1920

tactgtttct tttgtcgatg ctcaccctgt tgtttggtga tacttctgca gggt

1974

<210> 133

<211> 1010

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 133

gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat

60

ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag

120

atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc

180

taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg

240

gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta

300

tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg

360

gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa

420

actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt

480

acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg

540

ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg

600

ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt

660

atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga

720

tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata

780

tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat

840

aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc

900

agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact

960

gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcagggt

1010

<210> 134

<211> 2008

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 134

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca

60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac

120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagagggatca

180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt

240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata

300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga

360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact

420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca

480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag

540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc

600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg

660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt

720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc

780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc

840

gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc

900

aacacaccgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg

960

ccgctcatcc tccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg

1020

ttagggcccg gtagttctac ttctgttcat gtttgtgtta gagcaaacat gttcatgttc

1080

atgtttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag gatactgttt

1140

caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata

1200

gttacgagtt taagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc

1260

gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt

1320

ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt

1380

attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg

1440

atttattaaa ggatctgtat gtatgtgcct acatcttcat agttacgagt ttaagatgat

1500

ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat

1560

acagagatgc ttttttttcgc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag

1620

atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt

1680

gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg

1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat

1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa

1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt

1920

agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc

1980

ctgttgttgg gtgatacttc tgcaggtc

2008

<210> 135

<211> 877

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 135

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca

60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac

120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagagggatca

180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt

240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata

300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga

360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact

420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca

480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag

540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc

600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg

660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt

720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc

780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc

840

gtaataaata gacaccccct ccacaccctc ttttcccc

877

<210> 136

<211> 78

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 136

aacctcgtgt tcgttcggag cgcacacaca cgcaaccaga tctcccccaa atccagccgt

60

cggcacctcc gcttcaag

78

<210> 137

<211> 1053

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 137

gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc

60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca

120

tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac

180

tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct

240

tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt

300

gatgcgggtt ttactgatgc atatacagag atgctttttt tctcgcttgg ttgtgatgat

360

atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg

420

gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct

480

ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag

540

atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg

600

catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt

660

tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt

720

tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat

780

ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc

840

ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt

900

tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat

960

tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc

1020

tcaccctgtt gttgggtgat acttctgcag gtc

1053

<210> 138

<211> 2008

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 138

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca

60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac

120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagagggatca

180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt

240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata

300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga

360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact

420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca

480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag

540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc

600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg

660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt

720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc

780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc

840

gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc

900

aacacaccgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg

960

ccgctcatcc tccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg

1020

ttagggcccg gtagttctac ttctgttcat gtttgtgtta gagcaaacat gttcatgttc

1080

atgtttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag gatactgttt

1140

caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata

1200

gttacgagtt taagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc

1260

gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt

1320

ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt

1380

attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg

1440

atttattaaa ggatctgtat gtatgtgcct acatcttcat agttacgagt ttaagatgat

1500

ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat

1560

acagagatgc ttttttttcgc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag

1620

atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt

1680

gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg

1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat

1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa

1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt

1920

agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc

1980

ctgttgttgg gtgatacttc tgcaggggt

2008

<210> 139

<211> 1053

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 139

gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc

60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca

120

tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac

180

tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct

240

tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt

300

gatgcgggtt ttactgatgc atatacagag atgctttttt tctcgcttgg ttgtgatgat

360

atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg

420

gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct

480

ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag

540

atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg

600

catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt

660

tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt

720

tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat

780

ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc

840

ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt

900

tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat

960

tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc

1020

tcaccctgtt gttgggtgat acttctgcag ggt

1053

<210> 140

<211> 1635

<212> DNA

<213> Sorghum bicolor

<400> 140

ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat

60

ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct

120

tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa atagtactat ttttatttta

180

aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt

240

tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc

300

gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac

360

ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga cgtaaccctc cgttgcccac

420

gataaaagct ccacccccga ccccggcccc ccgatttccc ctacggacca gtctcccccc

480

gatcgcaatc gcgaattcgt cgcaccatcg gcacgcagac gaacgaagca aggctctccc

540

catcggctcg tcaaggtatg cgttccctag atttgttccc ttcctctctc ggtttgtcta

600

tatatatgca tgtatggtcg attcccgatc tcgtcgattc tcggtttcgc cttccgtacg

660

aagattcgtt tagattgttc atatgttctg ttgtgttacc agattgatcg gatcaacttg

720

atccagttat cttcgctcct ccgattagat ccgtttctat ttcagtatat atatactagt

780

atagtatcta gggttcacac tgttgaccga ctggttactt ggaattgatc cgtgctgagt

840

tcagttgttg ccgtccataa aggcccgtgc tattgtctgt tctgaaacga aatcctgtag

900

atttcttagg gttagtgttc aattcatcaa aaggttgatt agtgaattat caaatttgag

960

agggttaaat cattctcatc atgttgtctc gaatgtaatc ccaaagatat tatagactgt

1020

gtttcgattt gatggattga tttgtgtatc atctaaatca acaaggctaa gtcatcagtt

1080

catagaatca tgtttaggtt tccgttcaat agactagttt tatcaatata taaaattata

1140

agaagggtag ggtaaatcac gttgcctcaa atgccatcct gtatggtttg gtttcaattc

1200

aattagtttg gttgattagg gtatgctctg gattaagatg gttaaatctt ccctagcatc

1260

ttccctgcct atccttactt gatccgtttc ggatatgttg gaagtacagc gagcttattt

1320

catgttgata gtgacccctt tcagattata ctattgaata ttgtatgttt gccacttctg

1380

tatgttgaat tatcctgcta aattagcaat ggaattagca tattggcaat tggtatgcat

1440

ggacctaatc aggacggatg tggttatgtt agtttcaatt cattgtcaat tcattgttca

1500

cctgcgttag atatatatga tgatttttac gtgtagttca tagttcttga gttttggatc

1560

tttcttatct gatatatgct ttcctgtgcc tgtgctttat tgtgtcttac catgcgattt

1620

ttgtctatgc aggtc

1635

<210> 141

<211> 401

<212> DNA

<213> Sorghum bicolor

<400> 141

ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat

60

ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct

120

tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa atagtactat ttttatttta

180

aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt

240

tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc

300

gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac

360

ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga c

401

<210> 142

<211> 154

<212> DNA

<213> Sorghum bicolor

<400> 142

gtaaccctcc gttgccccg ataaaagctc cacccccgac cccggccccc cgatttcccc

60

tacggaccag tctccccccg atcgcaatcg cgaattcgtc gcaccatcgg cacgcagacg

120

aacgaagcaa ggctctcccc atcggctcgt caag

154

<210> 143

<211> 1080

<212> DNA

<213> Sorghum bicolor

<400> 143

gtatgcgttc cctagatttg ttcccttcct ctctcggttt gtctatatat atgcatgtat

60

ggtcgattcc cgatctcgtc gattctcggt ttcgccttcc gtacgaagat tcgtttagat

120

tgttcatatg ttctgttgtg ttaccagatt gatcggatca acttgatcca gttatcttcg

180

ctcctccgat tagatccgtt tctatttcag tatatatata ctagtatagt atctagggtt

240

cacactgttg accgactggt tacttggaat tgatccgtgc tgagttcagt tgttgccgtc

300

cataaaggcc cgtgctattg tctgttctga aacgaaatcc tgtagatttc ttagggttag

360

tgttcaattc atcaaaaggt tgattagtga attatcaaat ttgagagggt taaatcattc

420

tcatcatgtt gtctcgaatg taatcccaaa gatattatag actgtgtttc gatttgatgg

480

attgatttgt gtatcatcta aatcaacaag gctaagtcat cagttcatag aatcatgttt

540

aggtttccgt tcaatagact agttttatca atatataaaa ttataagaag ggtagggtaa

600

atcacgttgc ctcaaatgcc atcctgtatg gtttggtttc aattcaatta gtttggttga

660

ttagggtatg ctctggatta agatggttaa atcttcccta gcatcttccc tgcctatcct

720

tacttgatcc gtttcggata tgttggaagt acagcgagct tatttcatgt tgatagtgac

780

ccctttcaga ttatactatt gaatattgta tgtttgccac ttctgtatgt tgaattatcc

840

tgctaaatta gcaatggaat tagcatattg gcaattggta tgcatggacc taatcaggac

900

ggatgtggtt atgttagttt caattcattg tcaattcatt gttcacctgc gttagatata

960

tatgatgatt tttacgtgta gttcatagtt cttgagtttt ggatctttct tatctgatat

1020

atgctttcct gtgcctgtgc tttattgtgt cttaccatgc gatttttgtc tatgcaggtc

1080

<210> 144

<211> 2067

<212> DNA

<213> Sorghum bicolor

<400> 144

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc

60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata

120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag

180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa

240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta

300

gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga

420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg

480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca

540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc

600

ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac

660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact

720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct

780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc

840

atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc

900

aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag

960

tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta

1020

cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg

1080

gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt

1140

gatcgatcgc gatctgtgat ttcgttgcgc cttgtgtatg cttggagtga tctaggcttg

1200

tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga

1260

ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta

1320

ctccagtgct atattcacat tgtttgaggc ttgagttttg tcgatcagtg gtcatgagaa

1380

aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat

1440

gttttataga ccttgtggca tggctccttt gttctatgggg tgctttattt tcctgaataa

1500

cagtaatgcg agactggtct atgggtgctt tgaccagtaa tgcgagacta gttatttgat

1560

catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg

1620

gtttctacga tccttatcat tttacttctg aatgaattta tttatttaag atattacagt

1680

gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc

1740

ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat

1800

ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg

1860

ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg

1920

ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa

1980

cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag

2040

tttctttgtg tttgattgaa acaggtg

2067

<210> 145

<211> 855

<212> DNA

<213> Sorghum bicolor

<400> 145

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc

60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata

120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag

180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa

240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta

300

gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga

420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg

480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca

540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc

600

ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac

660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact

720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct

780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc

840

atccaggcaa ggcgc

855

<210> 146

<211> 136

<212> DNA

<213> Sorghum bicolor

<400> 146

agagcctcag accagattcc gatcaatcac ccataagctc cccccaaatc tgttcctcgt

60

ctcccgtctc gcggtttcct acttccctcg gacgcctccg gcaagtcgct cgaccgcgcg

120

attccgcccg ctcaag

136

<210> 147

<211> 1076

<212> DNA

<213> Sorghum bicolor

<400> 147

gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt

60

ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg

120

aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc

180

ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc

240

tttgtagagg ctgggggtct aggctgtgat tttagaatca aataaagctg ttccttaccg

300

tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct

360

tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat

420

tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg

480

ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt

540

gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac

600

aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga

660

atgaatttat ttatttaaga tattacagtg caataaactg ctgtataata tcagtaacaa

720

actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat

780

cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt

840

ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata

900

taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa

960

catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta

1020

aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa caggtg

1076

<210> 148

<211> 2067

<212> DNA

<213> Sorghum bicolor

<400> 148

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc

60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata

120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag

180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa

240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta

300

gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga

420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg

480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca

540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc

600

ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac

660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact

720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct

780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc

840

atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc

900

aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag

960

tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta

1020

cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg

1080

gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt

1140

gatcgatcgc gatctgtgat ttcgttgcgc cttgtgtatg cttggagtga tctaggcttg

1200

tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga

1260

ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta

1320

ctccagtgct atattcacat tgtttgaggc ttgagttttg tcgatcagtg gtcatgagaa

1380

aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat

1440

gttttataga ccttgtggca tggctccttt gttctatgggg tgctttattt tcctgaataa

1500

cagtaatgcg agactggtct atgggtgctt tgaccagtaa tgcgagacta gttatttgat

1560

catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg

1620

gtttctacga tccttatcat tttacttctg aatgaattta tttatttaag atattacagt

1680

gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc

1740

ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat

1800

ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg

1860

ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg

1920

ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa

1980

cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag

2040

tttctttgtg tttgattgaa acagggt

2067

<210> 149

<211> 1076

<212> DNA

<213> Sorghum bicolor

<400> 149

gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt

60

ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg

120

aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc

180

ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc

240

tttgtagagg ctgggggtct aggctgtgat tttagaatca aataaagctg ttccttaccg

300

tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct

360

tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat

420

tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg

480

ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt

540

gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac

600

aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga

660

atgaatttat ttatttaaga tattacagtg caataaactg ctgtataata tcagtaacaa

720

actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat

780

cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt

840

ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata

900

taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa

960

catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta

1020

aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa cagggt

1076

<210> 150

<211> 2003

<212> DNA

<213> Sorghum bicolor

<400> 150

agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa

60

aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata

120

agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat

180

ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat

240

gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa

300

cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc

360

cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtggggga

420

cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg

480

ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc

540

atcccctccc tgcctcatcc atccaaatcc cactccccaa tcccatcccg tcggagaaat

600

tcatcgaagc gaagcgaagc gaatcctccc gatcctctca aggtacgcga gttttcgaat

660

cccctccaga cccctcgtat gctttccctg ttcgttttcg tcgtagcgtt tgattaggta

720

tgctttccct gttcgtgttc gtcgtagggt tcgattaggt cgtgtgaggc catggcctgc

780

tgtgataaat ttatttgttg ttatatcgga tctgtagtcg atttgggggt cgtggtgtag

840

atccgcgggc tgtgatgaag ttatttggtg tgattgtgct cgcgtgattc tgcgcgttga

900

gctcgagtag atctgatggt tggacgaccg attggttcgt tggctggctg cgctaaggtt

960

gggctgggct catgttgcgt tcgctgttgc gcgtgattcc gcggatggac ttgcgcttga

1020

ttgccgccag atcacgttac gattatgtga tttcgtttgg aactttttag atttgtagct

1080

tctgcttatt atatgacaga tgcgcctact gctcatatgc ctgtggtaaa taatggatgg

1140

ctgtgggtca aactagttga ttgtcgagtc atgtatcata tacaggtgta tagacttgcg

1200

tctaattgtt tgcatgttgc agttatatga tttgttttag attgtttgtt ccactcatct

1260

aggctgtaaa agggacacta cttattagct tgttgtttaa tctttttatt agtagattat

1320

attggtaatg ttttactaat tattattatg ttatatgtga cttctgctca tgcctgatta

1380

taatcataga tcactgtagt tgattgttga atcatgtgtc aaatacccgt atacataaca

1440

ctacacattt gcttagttgt ttccttaact catgcaaatt gaacaccatg tatgatttgc

1500

atggtgctgt aatgttaaat actacagtcc tgttggtact tgtttagtaa gaatctgctt

1560

catacaacta tatgctatgc ctgatgataa tcatatatct ttgtgtaatt aataattagt

1620

tgactgttga ataatgtatc gagtacatac catggcacaa ttgcttagtc acttccttaa

1680

ccatgcatat tgaactgacc ccttcatgtt ctgctgaatt gttctattct gattagacca

1740

tacatcatgt attgcaatct ttatttgcaa ttgtaatgta atggttcggt tctcaaatgt

1800

taaatgctat agttgtgcta ctttctaatg ttaaatgcta tagctgtgct acttgtaaga

1860

tctgcttcat agtttagtta aattaggatg atgagctttg atgctgtaac tttgtttgat

1920

tatgttcata gttgatcagt ttttgttaga ctcacagtaa cttatggtct cactcttctt

1980

ctggtctttg atgtttgcag cgg

2003

<210> 151

<211> 565

<212> DNA

<213> Sorghum bicolor

<400> 151

agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa

60

aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata

120

agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat

180

ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat

240

gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa

300

cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc

360

cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtggggga

420

cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg

480

ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc

540

atcccctccc tgcctcatcc atcca

565

<210> 152

<211> 77

<212> DNA

<213> Sorghum bicolor

<400> 152

aatcccactc cccaatccca tcccgtcgga gaaattcatc gaagcgaagc gaagcgaatc

60

ctcccgatcc tctcaag

77

<210> 153

<211> 1361

<212> DNA

<213> Sorghum bicolor

<400> 153

gtacgcgagt tttcgaatcc cctccagacc cctcgtatgc tttccctgtt cgttttcgtc

60

gtagcgtttg attaggtatg ctttccctgt tcgtgttcgt cgtagggttc gattaggtcg

120

tgtgaggcca tggcctgctg tgataaattt atttgttgtt atatcggatc tgtagtcgat

180

ttgggggtcg tggtgtagat ccgcgggctg tgatgaagtt atttggtgtg attgtgctcg

240

cgtgattctg cgcgttgagc tcgagtagat ctgatggttg gacgaccgat tggttcgttg

300

gctggctgcg ctaaggttgg gctgggctca tgttgcgttc gctgttgcgc gtgattccgc

360

ggatggactt gcgcttgatt gccgccagat cacgttacga ttatgtgatt tcgtttggaa

420

ctttttagat ttgtagcttc tgcttattat atgacagatg cgcctactgc tcatatgcct

480

gtggtaaata atggatggct gtgggtcaaa ctagttgatt gtcgagtcat gtatcatata

540

caggtgtata gacttgcgtc taattgtttg catgttgcag ttatatgatt tgttttagat

600

tgtttgttcc actcatctag gctgtaaaag ggacactact tattagcttg ttgtttaatc

660

tttttattag tagattatat tggtaatgtt ttactaatta ttattatgtt atatgtgact

720

tctgctcatg cctgattata atcatagatc actgtagttg attgttgaat catgtgtcaa

780

atacccgtat acataacact acacatttgc ttagttgttt ccttaactca tgcaaattga

840

acaccatgta tgatttgcat ggtgctgtaa tgttaaatac tacagtcctg ttggtacttg

900

tttagtaaga atctgcttca tacaactata tgctatgcct gatgataatc atatatcttt

960

gtgtaattaa taattagttg actgttgaat aatgtatcga gtacatacca tggcacaatt

1020

gcttagtcac ttccttaacc atgcatattg aactgacccc ttcatgttct gctgaattgt

1080

tctattctga ttagaccata catcatgtat tgcaatcttt atttgcaatt gtaatgtaat

1140

ggttcggttc tcaaatgtta aatgctatag ttgtgctact ttctaatgtt aaatgctata

1200

gctgtgctac ttgtaagatc tgcttcatag tttagttaaa ttaggatgat gagctttgat

1260

gctgtaactt tgtttgatta tgttcatagt tgatcagttt ttgttagact cacagtaact

1320

tatggtctca ctcttcttct ggtctttgat gtttgcagcg g

1361

<210> 154

<211> 1812

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1812)

<223> Coding sequence with optimized

codons.

<400> 154

atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtggggca

60

ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa

120

gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt

180

cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttggggca

240

ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat

300

aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg

360

tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg

420

cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac

480

ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg

540

aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg

600

tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat

660

caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac

720

ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca

780

gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag

840

ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac

900

ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg

960

attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg

1020

gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct

1080

ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc

1140

aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa

1200

aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt

1260

gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg

1320

atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt

1380

gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg

1440

gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt

1500

atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg

1560

tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc

1620

agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata

1680

ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg

1740

gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga

1800

ggcaaacaat ga

1812

<210> 155

<211> 2001

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(2001)

<223> Chimeric coding sequence with processable

intron.

<400> 155

atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtggggca

60

ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa

120

gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt

180

cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttggggca

240

ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat

300

aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg

360

tatgttattg ccgggaaaag tgtacgtaag tttctgcttc tacctttgat atatatataa

420

taattatcat taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat

480

gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt

540

ttctaatata tgaccaaaat ttgttgatgt gcaggtatca ccgtttgtgt gaacaacgaa

600

ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag

660

cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac

720

accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt

780

aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt

840

gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg

900

aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa

960

agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag

1020

ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa

1080

gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta

1140

atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg

1200

ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt

1260

aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa

1320

gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg

1380

cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt

1440

ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg

1500

acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc

1560

gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat

1620

ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat

1680

cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac

1740

accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt

1800

gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg

1860

caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg

1920

aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg

1980

cagcagggag gcaaacaatg a

2001

<210> 156

<211> 1653

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1653)

<223> Coding sequence with optimized

codons.

<400> 156

atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga

60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt

120

gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc

180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta

240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt

300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt

360

tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa

420

aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga

480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat

540

tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga

600

tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg

660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt

720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt

780

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac

840

aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg

900

attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg

960

aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat

1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccggggc

1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa

1140

acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt

1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct

1260

ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct

1320

ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa

1380

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt

1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat

1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac

1560

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata

1620

aaggccaaga aggcgggaaa gtccaaattg taa

1653

<210> 157

<211> 936

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(936)

<223> Coding sequence with optimized

codons.

<400> 157

atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg

60

tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag

120

aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg

180

aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga

240

atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac

300

ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac

360

tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc

420

gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag

480

gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc

540

ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct

600

gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct

660

cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac

720

aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg

780

ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag

840

gtgaagggcc tccacttcag cggagaggac gctccagatg aaatgggtaa gtacatcaag

900

agcttcgtgg agcgcgtgct gaagaacgag cagtaa

936

<210> 158

<211> 253

<212> DNA

<213> Agrobacterium tumefaciens

<400> 158

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg

60

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc

120

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac

180

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct

240

atgttactag atc

253

<210> 159

<211> 210

<212> DNA

<213> Triticum aestivum

<400> 159

ctgcatgcgt ttggacgtat gctcattcag gttggagcca atttggttga tgtgtgtgcg

60

agttcttgcg agtctgatga gacatctctg tattgtgttt ctttccccag tgttttctgt

120

acttgtgtaa tcggctaatc gccaacagat tcggcgatga ataaatgaga aataaattgt

180

tctgattttg agtgcaaaaa aaaaggaatt

210

<210> 160

<211> 300

<212> DNA

<213> Oryza sativa

<400> 160

attaatcgat cctccgatcc cttaattacc ataccattac accatgcatc aatatccata

60

tatatataaa ccctttcgca cgtacttata ctatgttttg tcatacatat atatgtgtcg

120

aacgatcgat ctatcactga tatgatatga ttgatccatc agcctgatct ctgtatcttg

180

ttatttgtat accgtcaaat aaaagtttct tccacttgtg ttaataatta gctactctca

240

tctcatgaac cctatatata actagtttaa tttgctgtca attgaacatg atgatcgatg

300

<210> 161

<211> 1204

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1204)

<223> Group of chimeric transcriptional regulatory elements

expression.

<400> 161

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc

60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc

120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa

180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca

240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga

300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag

360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc

420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa

480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg

540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt

600

catttggaga ggacacgctg acaagctgac tctagcagat cctctagaac catcttccac

660

acactcaagc cacactattg gagaacacac agggacaaca caccataaga tccaagggag

720

gcctccgccg ccgccggtaa ccaccccgcc cctctcctct ttctttctcc gttttttttt

780

ccgtctcggt ctcgatcttt ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg

840

cgccgatc ggtgcgcggg aggggcggga tctcgcggct ggggctctcg ccggcgtgga

900

tccggcccgg atctcgcggg gaatggggct ctcggatgta gatctgcgat ccgccgttgt

960

tgggggagat gatggggggt ttaaaatttc cgccgtgcta aacaagatca ggaagagggg

1020

aaaagggcac tatggtttat atttttatat atttctgctg cttcgtcagg cttagatgtg

1080

ctagatcttt ctttcttctt tttgtgggta gaatttgaat ccctcagcat tgttcatcgg

1140

tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta

1200

gaag

1204

<210> 162

<211> 1396

<212> DNA

<213> Oryza sativa

<400> 162

tcgaggtcat tcatatgctt gagaagagag tcgggatagt ccaaaataaa acaaaggtaa

60

gattacctgg tcaaaagtga aaacatcagt taaaaggtgg tataaagtaa aatatcggta

120

ataaaaggtg gcccaaagtg aaatttactc ttttctacta ttataaaaat tgaggatgtt

180

tttgtcggta ctttgatacg tcatttttgt atgaattggt ttttaagttt attcgctttt

240

ggaaatgcat atctgtattt gagtcgggtt ttaagttcgt ttgcttttgt aaatacagag

300

ggatttgtat aagaaatatc tttagaaaaa cccatatgct aatttgacat aatttttgag

360

aaaaatatat attcaggcga attctcacaa tgaacaataa taagattaaa atagctttcc

420

cccgttgcag cgcatgggta ttttttctag taaaaataaa agataaactt agactcaaaa

480

catttacaaa aacaacccct aaagttccta aagcccaaag tgctatccac gatccatagc

540

aagccagcc caacccaacc caacccagcc caccccagtc cagccaactg gacaatagtc

600

tccacacccc cccactatca ccgtgagttg tccgcacgca ccgcacgtct cgcagccaaa

660

aaaaaaaaga aagaaaaaaa agaaaaagaa aaaacagcag gtgggtccgg gtcgtggggg

720

cgggaaacgc gaggaggatc gcgagccagc gacgaggccg gccctccctc cgcttccaaa

780

gaaacgcccc ccatcgccac tatatacata cccccccctc tcctcccatc cccccaaccc

840

taccaccacc accaccacca cctccacctc ctcccccctc gctgccggac gacgagctcc

900

tcccccctcc ccctccgccg ccgccgcgcc ggtaaccacc ccgcccctct ccctctttctt

960

tctccgtttt ttttccgtc tcggtctcga tctttggcct tggtagtttg ggtgggcgag

1020

aggcggcttc gtgccgccca gatcggtgcg cgggaggggc gggatctcgc ggctggctct

1080

cgcccccgtg gatccggccc ggatctcgcg gggaatgggg ctctcggatg tagatctgcg

1140

atccgccgtt gttggggccg atgatggggc ccttaaaatt tccgccgtgc taaacaagat

1200

caggaagagg ggaaaagggc actatggttt atatttttat atatttctgc tgcttcgtca

1260

ggcttagatg tgctagatct ttctttcttc tttttgtggg tagaatttaa tccctcagca

1320

ttgttcatcg gtagtttttc ttttcatgat tcgtgacaaa tgcagcctcg tgcggacgtt

1380

ttttgtagg tagaag

1396

<210> 163

<211> 1446

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1446)

<223> Group of chimeric transcriptional regulatory elements

expression.

<400> 163

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc

60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc

120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa

180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca

240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga

300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag

360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc

420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa

480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg

540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt

600

catttggaga ggacacgctg acaagctgac tctagcagat ctaccgtctt cggtacgcgc

660

tcactccgcc ctctgccttt gttactgcca cgtttctctg aatgctctct tgtgtggtga

720

ttgctgagag tggtttagct ggatctagaa ttacactctg aaatcgtgtt ctgcctgtgc

780

tgattacttg ccgtcctttg tagcagcaaa atatagggac atggtagtac gaaacgaaga

840

tagacctac acagcaatac gagaaatgtg taatttggtg cttagcggta tttatttaag

900

cacatgttgg tgttataggg cacttggatt cagaagtttg ctgttaattt aggcacaggc

960

ttcatactac atgggtcaat agtataggga ttcatattat aggcgatact ataataattt

1020

gttcgtctgc agagcttatt atttgccaaa attagatatt cctattctgt ttttgtttgt

1080

gtgctgttaa attgttaacg cctgaaggaa taaatataaa tgacgaaatt ttgatgttta

1140

tctctgctcc tttattgtga ccataagtca agatcagatg cacttgtttt aaatattgtt

1200

gtctgaagaa ataagtactg acagtatttt gatgcattga tctgcttgtt tgttgtaaca

1260

aaatttaaaa ataaagagtt tccttttttgt tgctctcctt acctcctgat ggtatctagt

1320

atctaccaac tgacactata ttgcttctct ttacatacgt atcttgctcg atgccttctc

1380

cctagtgttg accagtgtta ctcacatagt ctttgctcat ttcattgtaa tgcagatacc

1440

aagcgg

1446

<210> 164

<211> 675

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(675)

<223> Group of chimeric transcriptional regulatory elements

expression.

<400> 164

ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg

60

cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg

120

ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa

180

agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc

240

aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg

300

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa

360

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg

420

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag

480

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa

540

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat

600

ttcatttgga gaggaaccat cttccacaca ctcaagccac actattggag aacacacagg

660

gacaacacac cataa

675

<210> 165

<211> 804

<212> DNA

<213> Zea mays

<400> 165

accgtcttcg gtacgcgctc actccgccct ctgcctttgt tactgccacg tttctctgaa

60

tgctctcttg tgtggtgatt gctgagagtg gtttagctgg atctagaatt acactctgaa

120

atcgtgttct gcctgtgctg attacttgcc gtcctttgta gcagcaaaat atagggacat

180

ggtagtacga aacgaagata gaacctacac agcaatacga gaaatgtgta atttggtgct

240

tagcggtatt tatttaagca catgttggtg ttatagggca cttggattca gaagtttgct

300

gttaatttag gcacaggctt catactacat gggtcaatag tatagggatt catattatag

360

gcgatactat aataatttgt tcgtctgcag agcttattat ttgccaaaat tagatattcc

420

tattctgttt ttgtttgtgt gctgttaaat tgttaacgcc tgaaggaata aatataaatg

480

acgaaatttt gatgtttatc tctgctcctt tattgtgacc ataagtcaag atcagatgca

540

cttgttttaa atattgttgt ctgaagaaat aagtactgac agtattttga tgcattgatc

600

tgcttgtttg ttgtaacaaa atttaaaaat aaagagtttc ctttttgttg ctctccttac

660

ctcctgatgg tatctagtat ctaccaactg acactatatt gcttctcttt acatacgtat

720

cttgctcgat gccttctccc tagtgttgac cagtgttact cacatagtct ttgctcattt

780

cattgtaatg cagataccaa gcgg

804

<210> 166

<211> 623

<212> DNA

<213> Cauliflower mosaic virus

<400> 166

ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg

60

cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg

120

ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa

180

agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc

240

aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg

300

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa

360

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg

420

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag

480

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa

540

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat

600

ttcatttgggagaggacacgc tga

623

<210> 167

<211> 8

<212> DNA

<213> Cauliflower mosaic virus

<400> 167

acacgctg

8

<210> 168

<211> 1790

<212> DNA

<213> Coix lacryma-jobi

<400> 168

gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata

60

caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag

120

taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact

180

tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc

240

acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg

300

ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag

360

gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat

420

ttccttcccc tcccctgccg ttctcctcat cataaaatagc caccccctcc tcgcctcttt

480

tccccaactc atctgttctt cgtctcacac agccagatcc caatccctct cctcgcgaac

540

ttcgtcgatc tcccttccct cgcctcgctt caaggtacgg cgatcatcct cccgctttcc

600

ctcctcctcc tctagatgta gtacggagta cttgccatca tgcatcatgc tacatcacgc

660

tcgtgcgagc tctgggtcct cgatctggga acggaactgt gggatgctgc tcgtgcgatt

720

tattattgggg gatctgggtt ctcgatctgg gaacggaact gtgggatgct gctcgtgcga

780

ttttattattg gggatctggg ttctcgatct gggaacggaa ctgtgggatg cttgtaggca

840

ggtcggagat gggtcggatc gttgcttagg gttcgatctg ctcgtggttt tcttttaatc

900

cctgatgcat gatttatcgg tcatcctatt agatggaacc agtagggtga ctctgatccg

960

atatacttaa cctcgatctg gttcgatgtt cctggctagg cttgtgcgtc tgtttcgtca

1020

gaccagtttt gctgtttttg gtatggttgt gatgcccgtc caaatatgac taagcgagtg

1080

tagatcatt ttatgaacta actgctggtc ttattaaatc tagatctgca tacgttgatg

1140

tactacgttc atagttgata cagtatgtat gaactagttg ctggtcgtat taattttgga

1200

tctgcatgtg tggtagcata taatgttcat aatacaattg atacagtatg atgtatgaac

1260

tatctgctgg ttttattaaat ttggatctgc ttgtggtaaa aaatatgtttt tttatatagt

1320

taccatgatg gattaatcta tacttctgat gtatatgctg cagttttctg ctgaggctgt

1380

agttttttcc agattaaaat acagcatgca tatttgctaa gctctgggcg tgtgaacgcc

1440

caccatggca ttgtccagta atagtaatga atttttttgt ttgcctgatg tgggagaaaa

1500

cacgcattgt ccagttattt tgttccatat gcattgtcct gttttgttgg atatgcatgc

1560

ttagaaaaca tatgcagcca ctgtttgata atgctttagc atctgcctgt tgaacatgca

1620

tgatctacct atctttattt tgtatgtact tgggtagtgg catgttgcta gttttccttg

1680

attctgtggc gtctacatgt tgagcttgca tatatgtttg ttgtccttct tttcctcctt

1740

ggtctactgc tatatgctta cccttttgtt tggctaattt tcaggtgcag

1790

<210> 169

<211> 481

<212> DNA

<213> Coix lacryma-jobi

<400> 169

gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata

60

caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag

120

taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact

180

tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc

240

acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg

300

ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag

360

gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat

420

ttccttcccc tcccctgccg ttctcctcat cataaaatagc caccccctcc tcgcctcttt

480

t

481

<210> 170

<211> 93

<212> DNA

<213> Coix lacryma-jobi

<400> 170

ccccaactca tctgttcttc gtctcacaca gccagatccc aatccctctc ctcgcgaact

60

tcgtcgatct cccttccctc gcctcgcttc aag

93

<210> 171

<211> 1216

<212> DNA

<213> Coix lacryma-jobi

<400> 171

gtacggcgat catcctcccg ctttccctcc tcctcctcta gatgtagtac ggagtacttg

60

ccatcatgca tcatgctaca tcacgctcgt gcgagctctg ggtcctcgat ctgggaacgg

120

aactgtggga tgctgctcgt gcgatttatt attggggatc tgggttctcg atctgggaac

180

ggaactgtgg gatgctgctc gtgcgattta ttattgggga tctgggttct cgatctggga

240

acggaactgt gggatgcttg taggcaggtc ggagatgggt cggatcgttg cttagggttc

300

gatctgctcg tggttttctt ttaatccctg atgcatgatt tatcggtcat cctattagat

360

ggaaccagta gggtgactct gatccgatat acttaacctc gatctggttc gatgttcctg

420

gctaggcttg tgcgtctgtt tcgtcagacc agttttgctg tttttggtat ggttgtgatg

480

cccgtccaaa tatgactaag cgagtgtaga atcattttat gaactaactg ctggtcttat

540

taaatctaga tctgcatacg ttgatgtact acgttcatag ttgatacagt atgtatgaac

600

tagttgctgg tcgtattaat tttggatctg catgtgtggt agcatataat gttcataata

660

caattgatac agtatgatgt atgaactatc tgctggttta ttaaatttgg atctgcttgt

720

ggtaaaaaat atgtttttta tatagttacc atgatggatt aatctatact tctgatgtat

780

atgctgcagt tttctgctga ggctgtagtt ttttccagat taaaatacag catgcatatt

840

tgctaagctc tgggcgtgtg aacgcccacc atggcattgt ccagtaatag taatgaattt

900

ttttgtttgc ctgatgtggg agaaaacacg cattgtccag ttattttgtt ccatatgcat

960

tgtcctgttt tgttggatat gcatgcttag aaaacatatg cagccactgt ttgataatgc

1020

tttagcatct gcctgttgaa catgcatgat ctacctatct ttatttttgta tgtacttggg

1080

tagtggcatg ttgctagttt tccttgattc tgtggcgtct acatgttgag cttgcatata

1140

tgtttgttgt ccttcttttc ctccttggtc tactgctata tgcttaccct tttgtttggc

1200

taatttttcag gtgcag

1216

<---

Claims (31)

1. A recombinant DNA molecule for initiating transcription of a heterologous transcribed DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 168 or 169, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 168 or 169; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule. 2. A recombinant DNA molecule according to claim 1, characterized in that the heterologous transcribed DNA molecule is a gene of agronomic interest. 3. A recombinant DNA molecule according to claim 2, characterized in that the gene of agronomic interest causes plant tolerance to herbicides. 4. A recombinant DNA molecule according to claim 2, characterized in that the gene of agronomic interest causes resistance to pests in plants. 5. A genetic construct for initiating transcription of a heterologous transcribed DNA molecule, comprising a recombinant DNA molecule according to claim 1, wherein said DNA molecule is operably linked to the 3'UTR. 6. A transgenic plant cell containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 168 or 169, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 168 or 169; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and wherein said plant cell has increased expression of said heterologous transcribed DNA molecule compared to a plant cell lacking said recombinant DNA molecule. 7. Transgenic plant cell according to claim 6, characterized in that said transgenic plant cell is a monocotyledonous plant cell. 8. A transgenic plant cell according to claim 6, characterized in that said transgenic plant cell is a dicotyledonous plant cell. 9. A transgenic plant containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 168 or 169, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 168 or 169; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and wherein said transgenic plant has increased expression of said heterologous transcribed DNA molecule compared to a plant lacking said recombinant DNA molecule. 10. Part of a transgenic plant containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 168 or 169, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 168 or 169; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and where the specified part of the transgenic plant has an increased expression of the specified heterologous transcribed DNA molecule compared to the part of the plant that does not contain the specified recombinant DNA molecule. 11. A transgenic seed from a transgenic plant according to claim 9 to obtain a plant, where this seed contains the recombinant DNA molecule according to claim 1 and where the specified plant has an increased expression of the specified heterologous transcribed DNA molecule compared to a plant that does not contain the specified recombinant DNA molecule. 12. A method for expressing a heterologous transcribed DNA molecule, comprising the following steps: obtaining a transgenic plant according to claim 9 and cultivating said plant, wherein the transcribed DNA molecule is expressed. 13. A method for producing a transgenic plant, comprising the following steps: a) transforming a plant cell with a recombinant DNA molecule according to claim 1 to obtain a transformed plant cell; And b) regenerating the transgenic plant from the transformed plant cell; wherein said transgenic plant has increased expression of said heterologous transcribed DNA molecule compared to a plant lacking said recombinant DNA molecule.

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