RU2796556C2 - Regulatory elements of the plants and their use - Google Patents

Abstract

FIELD: biochemistry.

SUBSTANCE: invention relates to a recombinant DNA molecule for initiating transcription of a heterologous transcribed DNA molecule, as well as to a genetic construct for initiating transcription of a heterologous transcribed DNA molecule containing it. Also the following are disclosed: a cell of a transgenic plant, a transgenic plant, a part of a transgenic plant, a transgenic seed containing the abovementioned DNA molecule. The invention also relates to a method for expressing a heterologous transcribed DNA molecule, as well as a method for obtaining a transgenic plant using the abovementioned DNA molecule.

EFFECT: invention makes it possible to effectively initiate transcription of a heterologous transcribed DNA molecule in a plant.

13 cl, 9 dwg, 20 tbl, 7 ex

Description

LINK TO RELATED APPLICATIONS

[0001] This application claims priority over US Provisional Application No. 61/739720, filed Dec. 19, 2012, which is incorporated herein in its entirety by reference.

INCLUDING A SEQUENCE LIST

[0002] The sequence listing, which is contained in a file called "MONS323WOseq.txt", 345 kb in size (measured on Microsoft Windows®), and was created on December 17, 2013, is submitted in electronic form and is incorporated herein by reference .

FIELD OF TECHNOLOGY

[0003] The present invention relates to the field of plant molecular biology, plant genetic engineering, and DNA molecules useful for modulating gene expression in plants.

BACKGROUND OF THE INVENTION

[0004] Regulatory elements are genetic elements that regulate the activity of genes by modulating the transcription of an operably linked transcribed DNA molecule. Such elements include promoters, leaders, enhancers, introns, and 3' untranslated regions and are useful in the field of plant molecular biology and plant genetic engineering.

SUMMARY OF THE INVENTION

[0005] The invention provides novel regulatory elements for use in plants and genetic constructs containing the regulatory elements. The invention also provides transgenic plant cells, plants, plant parts and seeds containing regulatory elements. In one embodiment, the invention provides the regulatory elements described herein operably linked to a transcribed DNA molecule. In some embodiments, the transcribed DNA molecule is heterologous with respect to the sequence of the regulatory element provided herein. This document also provides methods for the production and use of the regulatory elements described herein, including genetic constructs containing regulatory elements, and transgenic plants, plant cells, plant parts, and seeds containing regulatory elements operably linked to a transcribed DNA molecule that is heterologous in in relation to the regulatory element.

[0006] Thus, in one aspect, the invention provides a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any of SEQ ID NOs: 1- 98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. By "heterologous transcribed DNA molecule" is meant that the transcribed DNA molecule is heterologous with respect to the DNA sequence. In specific embodiments, the recombinant DNA molecule contains a DNA sequence having at least 90 percent, at least 91 percent, at least 92 percent, at least 93 percent, at least 94 percent, at least about 95 percent, at least 96 percent, at least 97 percent, at least 98 percent, or at least 99 percent sequence identity to the DNA sequence of any of SEQ ID NOs: 1-98 and 168-171. In specific embodiments, the heterologous transcribed DNA molecule contains a gene of agronomic interest, such as a gene capable of conferring resistance to herbicides or pests in plants. In yet other embodiments, the invention provides a genetic construct comprising a recombinant DNA molecule as provided herein.

[0007] In another aspect, provided herein are transgenic plant cells comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any of SEQ ID Nos. : 1-98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. In some embodiments, the transgenic plant cell is a monocot plant cell. In other embodiments, the transgenic plant cell is a dicot cell.

[0008] In yet another aspect, provided herein is a transgenic plant, or portion thereof, comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of: a) a DNA sequence with at least about 85 percent sequence identity to any from SEQ ID Nos. 1-98 and 168-171; b) a DNA sequence containing any of SEQ ID nos: 1-98 and 168-171; and c) a fragment of any of SEQ ID nos: 1-98 and 168-171, and this fragment has a gene-regulatory activity; wherein the DNA sequence is operably linked to a heterologous transcribed DNA molecule. In specific embodiments of the invention, the transgenic plant is a daughter plant of any generation relative to the original transgenic plant and contains a recombinant DNA molecule. A transgenic seed containing a recombinant DNA molecule that produces such a transgenic plant when grown is also provided by the invention.

[0009] In yet another aspect, the invention provides a method for expressing a transcribed DNA molecule, e.g., a gene of agronomic interest, in a transgenic plant by obtaining a transgenic plant containing a recombinant DNA molecule of the invention and cultivating the plant.

[00010] This document also provides a method for providing a transgenic plant by transforming a plant cell with a recombinant DNA molecule of the invention to obtain a transformed plant cell and regenerating the transformed plant cell to obtain a transgenic plant.

BRIEF DESCRIPTION OF THE FIGURES

[00011] FIG. 1. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Agrostis nebulosa . In particular, Fig. 1 illustrates the alignment of the 2005 bp P-AGRne.Ubq1-1:1:5 promoter (SEQ ID NO: 2) included in the expression regulatory element (EXP) group EXP-AGRne.Ubq1:1 :7 (SEQ ID no: 1), with promoter variants P-AGRne.Ubq1-1:1:5. For example, a deletion at the 5' end of P-AGRne.Ubq1-1:1:5 produced the P-AGRne.Ubq1-1:1:4 promoter (SEQ ID NO: 6), a 999 bp sequence that is included in EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5). Another version of the promoter illustrated in FIG. 1 is P-AGRne.Ubq1-1:1:6 (SEQ ID NO: 8), a 762 bp sequence included in EXP-AGRne.Ubq1:1:9 (SEQ ID NO: 7).

[00012] FIG. 2. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Arundo donax . In particular, Fig. 2 illustrates a 4114 bp promoter alignment. P-ARUdo.Ubq1-1:1:4 (SEQ ID no: 10), included in the group of regulatory expression elements EXP-ARUdo.Ubq1:1:4 (SEQ ID no: 9), with P-ARUdo.Ubq1 promoter variants -1:1:4. The alignment includes: a 2012 bp promoter. P-ARUdo.Ubq1-1:1:5 (SEQ ID NO: 14); 1000 bp promoter P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); and a 755 bp promoter. P-ARUdo.Ubq1-1:1:8 (SEQ ID no: 22).

[00013] FIG. 3. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Arundo donax . In particular, Fig. 3 illustrates a 2033 bp promoter alignment. P-ARUdo.Ubq2-1:1:4 (SEQ ID NO: 24) with P-ARUdo.Ubq2-1:1:4 promoter variants. Included in the alignment are: a 2004 bp promoter. P-ARUdo.Ubq2-1:1:6 (SEQ ID NO: 28); 1001 bp promoter P-ARUdo.Ubq2-1:1:5 (SEQ ID no: 31); and a 696 bp promoter. P-ARUdo.Ubq2-1:1:7 (SEQ ID no: 33).

[00014] FIG. 4. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Bouteloua gracilis . In particular, Fig. 4 illustrates a 2371 bp promoter alignment. P-BOUgr.Ubq1-1:1:2 (SEQ ID NO: 35) with promoter variants at the 5'end of P-BOUgr.Ubq1-1:1:2. Included in the alignment are: 1999 bp promoter. P-BOUgr.Ubq1-1:1:3 (SEQ ID no: 39); 1022 bp promoter P-BOUgr.Ubq1-1:1:5 (SEQ ID no: 42); and a 760 bp promoter. P-BOUgr.Ubq1-1:1:6 (SEQ ID no: 44).

[00015] FIG. 5. Illustrates the alignment of several promoter variants of various sizes corresponding to promoter elements from Bouteloua gracilis . In particular, Fig. 5 illustrates the alignment of the 2100 bp promoter element. P-BOUgr.Ubq2-1:1:4 (SEQ ID NO: 46) with P-BOUgr.Ubq2-1:1:4 promoter variants. Included in the alignment: 2043 bp promoter. P-BOUgr.Ubq2-1:1:7 (SEQ ID no: 50); 2002 bp promoter P-BOUgr.Ubq2-1:1:5 (SEQ ID no: 53); 1024 bp promoter P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); and a 749 bp promoter. P-BOUgr.Ubq2-1:1:8 (SEQ ID no: 61).

[00016] FIG. 6. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Miscanthus sinensis . In particular, Fig. 6 illustrates the alignment of the 5359 bp promoter element. P-MISsi.Ubq1-1:1:2 (SEQ ID NO: 63) with P-MISsi.Ubq1-1:1:2 promoter variants. Included in the alignment are: 2423 bp promoter. P-MISsi.Ubq1-1:1:11 (SEQ ID no: 67); 1447 bp promoter P-MISsi.Ubq1-1:1:10 (SEQ ID no: 71); 899 bp promoter P-MISsi.Ubq1-1:1:13 (SEQ ID no: 73); 691 bp promoter P-MISsi.Ubq1-1:1:14 (SEQ ID no: 75); and a 506 bp promoter. P-MISsi.Ubq1-1:1:9 (SEQ ID no: 77).

[00017] FIG. 7. Illustrates the alignment of several promoter variants of various sizes corresponding to promoter elements from Schizachyium scoparium. In particular, Fig. 7 illustrates the alignment of the 2831 bp promoter element. P-SCHsc.Ubq1-1:1:12 (SEQ ID NO: 79) with P-SCHsc.Ubq1-1:1:12 promoter variants. Included in the alignment are: 2033 bp promoter. P-SCHsc.Ubq1-1:1:11 (SEQ ID no: 83); 1046 bp promoter P-SCHsc.Ubq1-1:1:10 (SEQ ID no: 85); and a 547 bp promoter. P-SCHsc.Ubq1-1:1:14 (SEQ ID no: 87).

[00018] FIG. 8. Illustrates the alignment of several promoter variants of various sizes, corresponding to promoter elements from Sorghastrum nutans . In particular, Fig. 8 illustrates the alignment of the 2218 bp promoter element. P-SORnu.Ubq1-1:1:4 (SEQ ID NO: 89) with P-SORnu.Ubq1-1:1:4 promoter variants. Included in the alignment are: 1964 bp promoter. P-SORnu.Ubq1-1:1:5 (SEQ ID no: 93); 1023 bp promoter P-SORnu.Ubq1-1:1:6 (SEQ ID no: 96); and a 724 bp promoter. P-SORnu.Ubq1-1:1:7 (SEQ ID no: 98).

[00019] FIG. 9. Illustrates the configurations of the expression cassettes of the invention.

BRIEF DESCRIPTION OF SEQUENCES

[00020] SEQ ID nos: 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41, 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108, 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150 and 168 are DNA sequences from regulatory expression element (EXP) groups containing a promoter sequence operably linked to a 5' leader sequence that is operably linked with the 5' intron sequence.

[00021] SEQ ID nos: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 and 169 are promoter sequences.

[00022] SEQ ID nos: 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152, and 170 are leader sequences.

[00023] SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 and 171 are intron sequences.

DETAILED DESCRIPTION OF THE INVENTION

[00024] The invention relates to DNA molecules having gene regulatory activity in plants. The nucleotide sequences of these DNA molecules are given in SEQ ID nos: 1-98 and 168-171. These DNA molecules are, for example, capable of influencing the expression of an operably linked transcribed DNA molecule in plant tissues and therefore regulating the expression of an operably linked transgene in transgenic plants. The invention also relates to methods for modifying, creating and using them. The invention also relates to compositions that include transgenic plant cells, plants, plant parts and seeds containing the recombinant DNA molecules of the invention, and to methods for their preparation and use.

[00025] The following definitions and methods are provided to better define the invention and to guide those skilled in the art in the practice of the invention. Unless otherwise indicated, the terms are to be understood in accordance with common usage by those skilled in the art.

DNA molecules

[00026] As used herein, the term "DNA" or "DNA molecule" refers to a double-stranded DNA molecule of cellular or synthetic origin, i.e., a polymer of deoxyribonucleotide bases. As used herein, the term "DNA sequence" refers to the nucleotide sequence of a DNA molecule. The nomenclature used in this document corresponds to that of 37 CFR 1.822 of the United States and is set forth in WIPO Standard ST.25 (1998) Schedules Appendix 2, Tables 1 and 3.

[00027] As used herein, a "recombinant DNA molecule" is a DNA molecule containing a combination of DNA molecules that would not naturally combine without human intervention. For example, a recombinant DNA molecule may be a DNA molecule that consists of at least two heterologous DNA molecules with respect to each other; a DNA molecule containing a DNA sequence that deviates from naturally occurring DNA sequences; or a DNA molecule that has been inserted into the host cell's DNA by genetic transformation.

[00028] As used herein, the term "sequence identity" refers to the degree to which two optimally aligned DNA sequences are identical. Optimal sequence alignment is created by manually aligning two DNA sequences, such as a reference sequence and another DNA sequence, to create the maximum number of matches between nucleotides while aligning sequences with corresponding internal nucleotide insertions, deletions, or breaks. As used herein, the term "reference sequence" refers to the DNA sequence given in SEQ ID nos: 1-98 and 168-171.

[00029] As used herein, the term "percent sequence identity" or "percent identity" or "% identity" is the proportion of identity multiplied by 100. The "proportion of identity" for a DNA sequence optimally aligned with the starting sequence is the number of matches between nucleotides in the optimal alignment divided by the total number of nucleotides in the reference sequence, for example , by the total number of nucleotides in the entire full-length reference sequence. Thus, in one embodiment, the invention provides a DNA molecule comprising a DNA sequence that, when optimally aligned with the reference sequence provided herein as SEQ ID NOs: 1-98 and 168-171, has at least about 85 percent identity. , at least about 86 percent identical, at least about 87 percent identical, at least about 88 percent identical, at least about 89 percent identical, at least about 90 percent identical, at least about 91 percent identical, at least about 92 percent identical, at least about 93 percent identical, at least about 94 percent identical, at least about 95 percent identical, at least about 96 percent identical, at least about 97 percent identical, at least about 98 percent identity, at least about 99 percent identity, or at least about 100 percent identity with the reference sequence.

Regulatory elements

[00030] Regulatory elements such as promoters, leaders, enhancers, introns, and transcription termination regions (or 3´ UTRs) play an essential role in overall gene expression in living cells. The term "regulatory element" as used herein refers to DNA molecules having a regulatory gene activity. The term "regulatory gene activity" as used herein refers to the ability to influence the expression of an operably linked transcribed DNA molecule, for example by affecting the transcription and/or translation of an operably linked transcribed DNA molecule. Regulatory elements such as promoters, leaders, enhancers and introns that function in plants are thus useful for modifying plant phenotypes through genetic engineering.

[00031] As used herein, an "expression regulatory element group" or "EXP" sequence may refer to a group of operably linked regulatory elements such as enhancers, promoters, leaders, and introns. Thus, a group of expression control elements may consist, for example, of a promoter operably linked to a 5' leader sequence, which in turn is operably linked to a 5' intron sequence.

[00032] Regulatory elements can be characterized by their gene expression pattern, e.g. by positive and/or negative influences such as constitutive expression or temporal, spatial, developmental, tissue, environmental, physiological, pathological, cell cycle and / or chemically sensitive expression, and any of their combinations, as well as quantitative or qualitative indicators. As used herein, the term "gene expression pattern" is any feature of the transcription of an operably linked DNA molecule into a transcribed RNA molecule. The transcribed RNA molecule may be translated to produce a protein molecule or may form an antisense or other regulatory RNA molecule such as double-stranded RNA (dsRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), miRNA (miRNA), and the like.

[00033] As used herein, the term "protein expression" is any feature of the translation of a transcribed RNA molecule into a protein molecule. The expression of a protein can be characterized by its temporal, spatial, developmental or morphological properties, as well as quantitatively or qualitatively.

[00034] A promoter is useful as a regulatory element for modulating the expression of an operably linked transcribed DNA molecule. As used herein, the term "promoter" refers generally to a DNA molecule that is involved in the recognition and binding of RNA polymerase II and other proteins, such as trans-acting transcription factors, to initiate transcription. The promoter may be derived from the 5'-untranslated region (5'-UTR) of the gene. Alternatively, promoters can be obtained synthetically or by manipulating the DNA molecule. Promoters can also be chimeric. Chimeric promoters are produced by the fusion of two or more heterologous DNA molecules. Promoters useful in the practice of the invention include SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, including fragments or variants thereof. In specific embodiments of the invention, such DNA molecules and any variants or derivatives thereof described herein are further defined as having promoter activity, i . capable of acting as a promoter in a host cell, such as a transgenic plant. In still further specific embodiments of the invention, the fragment may be defined as exhibiting the promoter activity that the original promoter molecule from which it was derived has, or the fragment may contain a "minimal promoter" that provides a basic level of transcription and includes a TATA box or an equivalent DNA sequence for recognition and binding of the RNA polymerase II complex to initiate transcription.

[00035] In one embodiment, the fragments are provided with the promoter sequence described herein. Promoter fragments may include promoter activity as described above and may be useful singly or in combination with other promoters and promoter fragments, for example in the design of chimeric promoters. Specific embodiments of the invention provide promoter fragments comprising at least about 50, at least about 75, at least about 95, at least about 100, at least about 125, at least about 150, at least about 175, at least about 200, at least about 225, at least about 250, at least about 275, at least about 300, at least about 500, at least about 600, at least about 700 , at least about 750, at least about 800, at least about 900, or at least about 1000 consecutive nucleotides, or more, of a DNA molecule having the promoter activity described herein. Methods for obtaining such fragments from the original promoter molecule are well known in the art.

[00036] Compositions derived from any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50 , 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, such as internal or 5' deletions, for example, can be obtained using methods well known in the art to improve or alter expression, including the removal of elements that have positive or negative effects on expression; doubling elements that have positive or negative effects on expression; and/or duplication or deletion of elements having tissue or cell specific effects on expression. Compositions derived from any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169 containing 3'-deletions in which the TATA-box element or its equivalent sequence The DNA and the underlying sequence removed can be used, for example, to create enhancer elements. Additional deletions can be made to remove any elements that have positive or negative, tissue-specific, cell-specific, or time-specific (such as, but not limited to, circadian rhythms) effects on expression. Any of the promoters shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61 , 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, and fragments or enhancers derived from them can be used to prepare compositions of chimeric regulatory elements, containing any of the promoters shown in SEQ ID nos: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169, and fragments or enhancers derived therefrom operably linked to other enhancers and promoters.

[00037] In accordance with the present invention, a promoter or promoter fragment can be analyzed for known promoter elements, ie, DNA sequence characteristics such as the TATA box and other known transcription factor binding site motifs. The identification of such known promoter elements can be used by one of skill in the art to design promoter variants having a similar expression pattern as the parent promoter.

[00038] As used herein, the term "leader" refers to a DNA molecule from the 5'-untranslated region (5'-UTR) of a gene and is defined in a general sense as the DNA segment between the transcription initiation site (CIT) and the starting site of the protein coding sequence . Alternatively, the leaders can be obtained synthetically or by manipulating DNA elements. The leader can be used as a 5' regulatory element to modulate the expression of an operably linked transcribed DNA molecule. The leader molecules can be used with a heterologous promoter or with their native promoter. The promoter molecules of the invention may thus be operably linked to their native leader or may be operably linked to a heterologous leader. Leaders useful in the practice of the invention include SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170, or fragments or variants thereof. In specific embodiments of the invention, such DNA sequences can be defined as capable of acting as a leader in a host cell, including, for example, a transgenic plant cell. In one embodiment of the invention, such DNA sequences can be decoded as including leader activity.

[00039] The leader sequences (5'-UTRs) shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170 may be composed of regulatory elements or may take on secondary structures, affecting the transcription or translation of an operably linked DNA molecule. The leader sequences shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90 and 170 can be used according to the invention to create chimeric regulatory elements that affect transcription or translation of an operably linked DNA molecule. In addition, the leader sequences shown in SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, and 170 can be used to create chimeric leader sequences that affect transcription or translation of an operably linked DNA molecule. .

[00040] As used herein, the term "intron" refers to a DNA molecule that can be isolated or identified from a genomic copy of a gene and can be defined in a general sense as a region that is excised during messenger RNA (mRNA) processing prior to translation. Alternatively, the intron can be obtained synthetically or by manipulating a DNA element. The intron may contain enhancer elements that affect the transcription of functionally related genes. An intron can be used as a regulatory element to modulate the expression of an operably linked transcribed DNA molecule. The genetic construct may contain an intron, and the intron may or may not be heterologous to the DNA molecule being transcribed. Examples of introns in the art include the rice actin intron and the maize HSP70 intron.

[00041] Compared to genetic constructs without an intron, the inclusion of certain introns in genetic constructs results in increased accumulation of mRNA and protein in plants. This effect has been termed "intron mediated enhancement" (IME) of gene expression. Introns that stimulate expression in plants have been identified in maize genes ( e.g. tubA1, Adh1, Sh1, and Ubi1), in rice genes ( e.g. tpi ), and in genes of dicotyledonous plants such as petunia ( e.g. rbcS ), potato ( st -ls1 ) and Arabidopsis thaliana ( ubq3 and pat1 ) . Deletions or mutations within intron splicing sites have been shown to reduce gene expression, indicating that splicing may be required for IME. However, splicing per se is not required, as IME has been shown in dicotyledonous plants by point mutations within the splicing sites of the pat1 gene from A. thaliana . It has been shown that multiple use of the same intron in the same plant exhibits disadvantages. In such cases, it is necessary to have a collection of basic controls to generate the appropriate recombinant DNA elements.

[00042] Introns useful in the practice of the invention include SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91 , 94 and 171. Compositions derived from any of the introns shown in SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81 , 91, 94 and 171 may consist of internal deletions or duplications of cis-regulatory elements; and/or alterations to the 5' and 3' DNA sequences containing intron/exon splice junctions can be used to improve expression or expression specificity when operably linked to a promoter+leader or chimeric promoter+leader and a coding sequence. When changing intron/exon boundary sequences, it may be beneficial to avoid using the AT nucleotide sequence or A nucleotide sequence immediately before the 5' end of the splice site (GT) and the G nucleotide or TG nucleotide sequence, respectively, immediately after the 3' end of the splicing site (AG) , to eliminate potential unwanted start codons generated during the processing of the messenger RNA into the final transcript. The DNA sequence near the 5' or 3' end of the intron splicing sites can be modified in this way. Introns and intron variants altered as described herein and using methods known in the art can be tested empirically, as described in the Working Examples, to determine the effect of an intron on the expression of an operably linked DNA molecule.

[00043] As used herein, the term "3' transcription termination molecule", "3' untranslated region", or "3' UTR" refers to a DNA molecule that is used during transcription from the untranslated region of the 3' portion of an mRNA molecule . The 3' untranslated region of an mRNA molecule can be obtained by specific cleavage and 3' polyadenylation, also known as a poly(A) tail. The 3'-UTR may be operably linked to and downstream of the transcribed DNA molecule, and may include a polyadenylation signal and other regulatory signals capable of affecting transcription, mRNA processing, or gene expression. It is believed that poly(A) tails perform the functions of mRNA stabilization and translation initiation. Examples of 3' transcription termination molecules in the art are the nopaline synthase 3' region, the wheat hsp17 3' region, the pea RuBisCO small subunit 3' region, the cotton E6 3' region, and the coli 3' UTR.

[00044] 3'-UTRs generally find useful use for recombinant expression of specific DNA molecules. A weak 3'-UTR can generate a read-through that can affect the expression of a DNA molecule located in adjacent expression cassettes. A suitable control for transcription termination can prevent read-through into downstream DNA sequences ( eg other expression cassettes) and can further enable efficient reuse of RNA polymerase to improve gene expression. Efficient transcription termination (release of RNA polymerase II from DNA) is a prerequisite for the next transcription initiation and thus directly affects the overall transcript level. After transcription termination, the mature mRNA is released from the site of synthesis, and the template is transported into the cytoplasm. Eukaryotic mRNAs accumulate in poly(A) forms in vivo , making it difficult to detect transcription termination sites by conventional methods. However, prediction of functional and effective 3'-UTRs using bioinformatics methods is difficult, as there are no conserved DNA sequences that allow easy prediction of effective 3'-UTRs.

[00045] From a practical point of view, it is generally useful that the 3'-UTR used in the expression cassette has the following characteristics. The 3'-UTR must be able to rationally and efficiently complete transcription of the transgene and prevent read-through of the transcript in any of the adjacent DNA sequences that may be contained in another expression cassette, as in the case of several expression cassettes belonging to the same transfer DNA (T-DNA) , or adjacent chromosomal DNA into which the T-DNA has been inserted. The 3'-UTR should not lead to a decrease in the transcriptional activity conferred by the promoter, leader, enhancers and introns that are used to control the expression of the DNA molecule. In plant biotechnology, the 3'-UTR is often used to prime amplification reactions of reverse transcribed RNA isolated from transformed plants and is used to: (1) assess the transcriptional activity or expression of an expression cassette integrated into the plant chromosome; (2) estimates of the number of copies of inserts in plant DNA; and (3) assessing the zygosity of the resulting seed after propagation. The 3'-UTR is also used in amplification reactions of DNA extracted from transformed plants to determine the integrity of the inserted cassette.

[00046] As used herein, the term "enhancer" or "enhancer element" refers to a cis-acting regulatory element, referred to as a cis element, that confers an aspect of general character to expression, but generally not sufficient on its own to control transcription, functionally linked DNA sequence. Unlike promoters, enhancer elements typically do not include a transcription initiation site (TIT) or a TATA box or equivalent DNA sequence. A promoter or promoter fragment may naturally contain one or more enhancer elements that affect the transcription of an operably linked DNA sequence. An enhancer element can also be combined with a promoter to provide a chimeric cis promoter element conferring an aspect of general modulation of gene expression.

[00047] Many promoter enhancer elements are believed to bind DNA binding proteins and/or influence DNA topology by producing local conformations that selectively allow or restrict access of RNA polymerase to the DNA template, or that facilitate selective opening of the double helix at the initiation site. transcription. An enhancer element may also function to bind transcription factors that regulate transcription. Some enhancer elements bind more than one transcription factor, and transcription factors can interact with different affinities with more than one enhancer domain. Enhancer elements can be identified by a number of methods, including analysis of deletions, ie removal of one or more nucleotides from the 5' end or within the promoter; DNA binding protein analysis using DNase I footprinting, methylation interference, electrophoretic mobility shift assays, in vivo genomic footprinting by ligation-mediated polymerase chain reaction (PCR) and other conventional assays; or by DNA sequence similarity analysis using known cis element or enhancer element motifs as target sequence or target motif with conventional DNA sequence comparison methods such as BLAST. The fine structure of the enhancer domain can be further explored by mutagenesis (or substitution) of one or more nucleotides, or by other conventional methods known in the art. Enhancer elements can be obtained by chemical synthesis or by isolation from regulatory elements that include such elements, and they can be synthesized with additional flanking nucleotides containing applicable restriction sites to facilitate subsequent manipulations. Thus, the design, construction and use of enhancer elements, in accordance with the methods described herein, to modulate the expression of operably linked transcribed DNA molecules are within the scope of the invention.

[00048] As used herein, the term "chimeric" refers to a single DNA molecule obtained by fusing a first DNA molecule with a second DNA molecule, where neither the first nor the second DNA molecule would normally be contained in such a configuration,those. will not be merged with each other. A chimeric DNA molecule is thus a new DNA molecule, in other words, not normally found in nature. As used herein, the term "chimeric promoter" refers to a promoter obtained by such manipulation of DNA molecules. A chimeric promoter may combine two or more DNA fragments, such as fusion of a promoter with an enhancer element. Thus, the design, construction and use of chimeric promoters, in accordance with the methods described herein, to modulate the expression of operably linked transcribed DNA molecules are within the scope of the invention.

[00049] As used herein, the term "variant" refers to a second DNA molecule, such as a regulatory element, that is similar in composition to, but not identical to, the first DNA molecule, and wherein the second DNA molecule still retains overall functionality, i . the same or similar expression pattern, for example, through greater or lesser or equivalent transcriptional or translational activity compared to the first DNA molecule. The variant may be a shortened or truncated version of the first DNA molecule and/or an altered version of the DNA sequence of the first DNA molecule, such as a variant with different restriction sites and/or internal deletions, substitutions and/or insertions. "Variants" of a regulatory element also encompasses variants resulting from mutations that occur during or as a result of transformation of bacterial and plant cells. In the invention, the DNA sequence given in SEQ ID NOs: 1-98 and 168-171 can be used to generate variants that are similar in composition, but not identical to the DNA sequence of the original regulatory element, while still retaining common functionality, i.e. e . the same or similar pattern of expression as the original regulatory element. The preparation of such embodiments of the invention is within the ordinary skill of the art in light of the description and is within the scope of the invention.

[00050] Chimeric regulatory elements can be designed to include various constituent elements that can be operably linked in various ways known in the art, such as restriction enzyme digestion and ligation, ligation-independent cloning, modular assembly of PCR products during amplification, or direct chemical synthesis of the regulatory element, as well as by other methods known in the art. The resulting different chimeric regulatory elements may be composed of the same or variants of the same building blocks, but differ in DNA sequence, or DNA sequences that include a DNA binding sequence, or sequences that allow the building blocks to be operably linked. In the invention, the DNA sequence set forth in SEQ ID NOs: 1-98 and 168-171 may provide a regulatory element reference sequence in which constituent elements comprising the reference sequence may be joined by methods known in the art and may include substitutions, deletions and/or insertions of one or more nucleotides or mutations that are commonly encountered during the transformation of bacterial and plant cells.

[00051] The effectiveness of the modifications, duplications or deletions described herein on the desired aspects of the expression of a particular transgene can be tested empirically in stable and transient plant tests, such as those described in the working examples herein, in order to confirm the results. , which may vary depending on the changes made and the purpose of the change in the original DNA molecule.

genetic constructs

[00052] As used herein, the term "genetic construct" means any recombinant DNA molecule, such as a plasmid, cosmid, virus, phage, or linear or circular DNA or RNA molecule, obtained from any source, capable of genomic integration or autonomous replication, including a DNA molecule, where at least one DNA molecule is connected to another DNA molecule in a functionally effective manner, i.e., functionally linked to each other. As used herein, the term "vector" means any genetic construct that can be used for transformation purposes, i . introduction of heterologous DNA or RNA into the host cell. The genetic construct typically includes one or more expression cassettes. As used herein, "expression cassette" refers to a DNA molecule containing at least a transcribed DNA molecule operably linked to one or more regulatory elements, typically at least a promoter and a 3'-UTR.

[00053] As used herein, the term "operably linked" refers to a first DNA molecule connected to a second DNA molecule, wherein the first and second DNA molecules are arranged such that the first DNA molecule affects the function of the second DNA molecule. The two DNA molecules may or may not be part of a single continuous DNA molecule and may or may not be adjacent. For example, a promoter is operably linked to a transcribed DNA molecule if the promoter modulates the transcription of the target transcribed DNA molecule in the cell. A leader, for example, is operably linked to a DNA sequence when it is capable of affecting transcription or translation of the DNA sequence.

[00054] The genetic constructs of the invention can be provided in one embodiment as genetic constructs consisting of two tumor-inducing plasmid border regions, (Ti), having right border region (RB or AGRtu.RB) and left border region (LB or AGRtu.LB) Ti-plasmid isolated from Agrobacterium tumefaciens containing T-DNA, which, along with the molecular transfer provided by A. tumefaciens cells, allows the integration of T-DNA into the plant cell genome ( see, for example , US Pat. No. 6,603,061 ). Genetic constructs may also contain segments of a plasmid DNA backbone that provide replication function and selection for an antibiotic in bacterial cells, such as an Escherichia coli origin of replication such as ori322, a broad host-range origin of replication such as oriV or oriRi, and a selectable marker coding region. , such as Spec/Strp encoding a Tn7 aminoglycoside adenyltransferase ( aadA ) conferring resistance to spectinomycin or streptomycin, or a gentamicin selectable marker gene (Gm, Gent). A. tumefaciens bacterial host strain ABI, C58 or LBA4404 is often used for plant transformation; however, other strains known to those skilled in the art of plant transformation may function in the invention.

[00055] Methods are known in the art for assembling and introducing genetic constructs into a cell such that a transcribed DNA molecule is transcribed into a functional mRNA molecule that is translated and expressed as a protein. For the practice of the invention, conventional compositions and methods for the preparation and use of genetic constructs and host cells are well known to those skilled in the art. Typical vectors used to express nucleic acids in higher plants are well known in the art and include vectors derived from Agrobacterium tumefaciens Ti plasmid and pCaMVCN transfer control vector.

[00056] Various regulatory elements may be included in the genetic construct, including any of those proposed herein. Any such regulatory elements may be provided in combination with other regulatory elements. Such combinations can be designed or modified to obtain the desired regulatory functions. In one embodiment, the genetic constructs of the invention comprise at least one regulatory element operably linked to a transcribed DNA molecule operably linked to a 3'UTR.

[00057] The genetic constructs of the invention may include any promoter or leader as provided herein or known in the art. For example, a promoter of the invention may be operably linked to a heterologous untranslated 5' leader, such as one derived from a heat shock protein gene. Alternatively, the leader of the invention may be operably linked to a heterologous promoter, such as the cauliflower mosaic virus 35S promoter transcript.

[00058] Expression cassettes may also include a transit peptide coding sequence that encodes a peptide useful for intracellular targeting of an operably linked protein, particularly to a chloroplast, leukoplast, or other plastid organelle, mitochondria, peroxisome, vacuole, or extracellular space. Many proteins localized in chloroplasts are expressed as precursors by nuclear genes and are directed to the chloroplast via the chloroplast transit peptide (CTP). Examples of such proteins isolated from chloroplasts include, but are not limited to, ribulose-1,5-bisphosphate carboxylase small subunit (MCE)-associated proteins, ferredoxin, ferredoxin oxidoreductase, photosensitizing complex protein I and protein II, thioredoxin F, and enolpyruvil shikimate phosphate synthase (EPSFS). Chloroplast transit peptides are described, for example, in US Pat. No. 7,193,133. It has been shown that non-chloroplast proteins can be targeted to the chloroplast upon expression of a heterologous CTP operably linked to a transgene encoding non-chloroplast proteins.

transcribed DNA molecule

[00059] As used herein, the term "transcribable DNA molecule" refers to any DNA molecule capable of being transcribed into an RNA molecule, including, but not limited to, those that have protein-coding sequences and those that produce RNA molecules having sequences useful for gene suppression. The type of DNA molecule may include, but is not limited to, a DNA molecule from the same plant, a DNA molecule from another plant, a DNA molecule from another organism, or a synthetic DNA molecule such as a DNA molecule containing an antisense gene sequence or a DNA molecule encoding an artificial , a synthetic or otherwise modified version of the transgene. An exemplary transcribed DNA molecule for inclusion in the genetic constructs of the invention includes, for example , DNA molecules or genes from a different species than the one in which the DNA molecule is inserted, or genes derived from or present in the same species, but which are included in cells - recipients by means of genetic engineering, and not by classical methods of reproduction.

[00060] "Transgene" refers to a transcribed DNA molecule that is heterologous to a host cell, at least in terms of its location in the host cell's genome and/or a transcribed DNA molecule artificially incorporated into the host cell's genome in the current or any previous generation of cells.

[00061] A regulatory element, such as a promoter of the invention, may be operably linked to a transcribed DNA molecule that is heterologous to the regulatory element. As used herein, the term "heterologous" refers to a combination of two or more DNA molecules when such a combination does not normally occur in nature. For example, the two DNA molecules may be from different species and/or the two DNA molecules may be from different genes, such as different genes from the same species or the same genes from different species. A regulatory element is thus heterologous to an operably linked transcribed DNA molecule if such combination does not normally occur in nature, i.e. the transcribed DNA molecule does not normally occur operably linked to the regulatory element.

[00062] A transcribed DNA molecule can generally be any DNA molecule for which expression of a transcript is desired. Such transcript expression can lead to translation of the resulting mRNA molecule and, accordingly, to protein expression. Alternatively, for example, a transcribed DNA molecule can be designed to ultimately cause a decrease in the expression of a particular gene or protein. In one embodiment of the invention, this can be achieved with a transcribed DNA molecule oriented in the antisense direction. One of skill in the art is familiar with the use of such antisense technology. Any gene can be negatively regulated in this way, and in one embodiment of the invention, the transcribed DNA molecule can be designed to suppress a particular gene through the expression of a dsRNA, siRNA, or microRNA molecule.

[00063] Thus, in one embodiment, there is provided a recombinant DNA molecule comprising a regulatory element of the invention, such as those set forth in SEQ ID NOs: 1-98 and 168-171, operably linked to a heterologous transcribed DNA molecule for in order to modulate the transcription of the transcribed DNA molecule at the desired level or pattern when the genetic construct is integrated into the genome of the transgenic plant cell. In one embodiment of the invention, the transcribed DNA molecule contains the region of the gene encoding the protein, and in another embodiment of the invention, the transcribed DNA molecule contains the antisense region of the gene.

Genes of agronomic interest

[00064] The DNA molecule to be transcribed may be a gene of agronomic interest. As used herein, the term "gene of agronomic interest" refers to a transcribed DNA molecule that, when expressed in a particular plant tissue, cell, or cell type, confers a desired characteristic. A gene product of agronomic interest may act within a plant to influence plant morphology, physiology, growth, development, yield, grain composition, nutritional profile, disease or pest resistance, and/or environmental or chemical tolerance. , or may act as a pesticidal agent in the diet of a pest that feeds on the plant. In one embodiment, the regulatory element of the invention is incorporated into a genetic construct such that the regulatory element is operably linked to a transcribed DNA molecule that is a gene of agronomic interest. In a transgenic plant containing such a genetic construct, expression of a gene of agronomic interest can confer a beneficial agronomic trait. A beneficial agronomic trait may include, but is not limited to, for example, herbicide tolerance, insect control, yield modification, disease resistance, pathogen resistance, plant growth and development modification, modified starch content, modified oil content, modified fatty acid content , modified protein content, modified fruit ripening, improved animal and human nutrition, biopolymer production, environmental stress tolerance, pharmaceutical peptides, improved processing properties, improved taste, beneficial hybrid seed production, improved fiber production, and desirable biofuel production.

[00065] Examples of genes of agronomic interest known in the art include herbicide resistance (US Pat. Nos. 6,803,501, 6,448,476, 6,248,876, 6,225,114, yield (U.S. Patent No. Nos. USRE38446, 6716474, 6663906, 6476295, 6441277, 6423828, 6399330, 6372211, 6235971, 6222098 and 5716837), insect control (U.S. Patent Nos. 6809078, 6713063, 6686452, 6657046, 6645497, 6642030, 6639054, 6620988, 6593293, 6555655 6538109 6537756 6521442 6501009 6468523 6326351 6313378 6284949 6281016 6248536 6242241 6221649 6177 615 6156573 6153814 6110464 6093695 6063756 6063597 6023013 5959091 5942664 5942658 5880275 5,763,245 and 5,763,241), resistance to fungal diseases (US Pat. resistance to viruses (US patents No. 6617496, 6608241, 6015940, 6013864 , 5,850,023 and 5,304,730), nematode resistance (US Pat. No. 6,228,992), bacterial disease resistance (US Pat. No. 5,516,671), plant growth and development (US Pat. Nos. 6,723,897 and 6,518,488), starch accumulation (US Pat. 6538179, 6538178, 5750876, 6476295), production of modified oils (US patents Nos. 6444876, 6426447 and 6380462), increased production of oils (US patents Nos. fatty acid content (U.S. Patent Nos. 6828475, 6822141, 6770465, 6706950, 6660849, 6596538, 6589767, 6537750, 6489461 and 6459018), increased protein production (US patent No. 6380466), fruit ripening (US patent No. 55124 66), improved animal and human nutrition (U.S. Patent Nos. 6723837, 6653530, 6541259; 5,985,605 and 6,171,640), biopolymers (US Pat. 6140075 and 6,080,560), improved processing properties (U.S. Patent No. 6,476,295), improved digestibility (U.S. Patent No. 6,531,648), low raffinose (U.S. Patent No. 6,166,292), commercial production of enzyme (U.S. Patent No. 6,011,199), nitrogen fixation (U.S. Patent No. 5,229,114), hybrid seed production (U.S. Patent No. 5,689,041), fiber production (U.S. Patents Nos. 6,576,818, 6,271,443, 5,981,834 and 5,869,720), and biofuel production (U.S. Patent No. 5,998,700).

[00066] Alternatively, a gene of agronomic interest can affect the aforementioned plant characteristics or phenotypes by encoding an RNA molecule that causes targeted modulation of endogenous gene expression, such as through antisense ( see, for example , US Pat. No. 5,107,065), inhibitory RNA ("RNA interferences" involving the modulation of gene expression by microRNA, miRNA, trans-acting miRNA and phasic small RNA-mediated mechanisms, e.g. as described in US Published Applications 2006/0200878 and 2008/0066206 and in US Patent Application 11/974469); or cosuppressor-mediated mechanisms. The RNA can also be a catalytic RNA molecule ( eg ribozyme or riboswitch, see eg US Published Application 2006/0200878) designed to cleave the desired endogenous mRNA product. Methods are known in the art for designing and introducing genetic constructs into a cell such that a transcribed DNA molecule is transcribed into a molecule capable of inducing gene suppression.

[00067] Expression of a transcribed DNA molecule in a plant cell can also be used to suppress plant pests that feed on plant cells, such as compositions isolated from beetle pests and compositions isolated from nematode pests. Plant pests include, but are not limited to, arthropod pests, nematode pests, and fungal or microbial pests.

selective markers

[00068] Selectable marker transgenes can also be used with the regulatory elements of the invention. As used herein, the term "selectable marker transgene" refers to any transcribed DNA molecule whose expression in the transgenic plant, tissue or cell, or lack thereof, can be screened or counted by some method. Selectable marker genes and associated selection and screening methods for use in the practice of the invention are known in the art and include, but are not limited to, transcribed DNA molecules encoding beta-glucuronidase (GUS), green fluorescent protein ( GFP), proteins conferring antibiotic resistance and proteins conferring herbicide resistance.

Cell transformation

[00069] The invention is also directed to a method for producing transformed cells and plants containing one or more regulatory elements operably linked to the transcribed DNA molecule.

[00070] The term "transformation" refers to the introduction of a DNA molecule into a recipient host. As used herein, the term "host" refers to bacteria, fungi, or plants, including any cells, tissues, organs, or progeny of bacteria, fungi, or plants. Plant tissues and cells of particular interest include protoplasts, callus, roots, tubers, seeds, stems, leaves, seedlings, ovaries and pollen.

[00071] As used herein, the term "transformed" refers to a cell, tissue, organ, or organism into which a foreign DNA molecule, such as a genetic construct, has been introduced. The introduced DNA molecule can be integrated into the genomic DNA of a cell, tissue, organ or organism of the recipient such that the introduced DNA molecule is inherited in future offspring. A "transgenic" or "transformed" cell or organism may also include the progeny of the cell or organism and the progeny resulting from a breeding program using such a transgenic organism as the parent in the cross and exhibiting an altered phenotype as a result of the presence of foreign DNA molecules. The introduced DNA molecule may also be temporarily introduced into the recipient cell such that the introduced DNA molecule is not inherited in further offspring. The term "transgenic" refers to a bacterium, fungus or plant containing one or more heterologous DNA molecules.

[00072] There are many methods, well known to those skilled in the art, for introducing a DNA molecule into plant cells. The process typically includes the steps of selecting an appropriate host cell, transforming the host cell with a vector, and obtaining the transformed host cell. Methods and materials for transforming plant cells, by introducing a plant genetic construct into the plant genome, in practice of the invention may include any of the known and shown methods. Suitable methods include, but are not limited to, bacterial infection ( e.g. , Agrobacterium ), binary IIR vectors, direct DNA transfer ( e.g. , PEG-mediated transformation, drying/inhibition-mediated DNA uptake, electroporation, mixing with silicon carbide fibers, and particle acceleration covered with DNA) from among others.

[00073] The host cells can be any cell or organism, such as a plant cell, an algal cell, an algae, a fungal cell, a fungus, a bacterial cell, or an insect cell. In specific embodiments of the invention, host cells and transformed cells may include cultured plant cells.

[00074] The transgenic plant can subsequently be regenerated from the transgenic plant cell of the invention. Seeds can be obtained from this transgenic plant using conventional propagation or self-pollination techniques. Such seed and the resulting progeny plant grown from such seed will contain the recombinant DNA molecule of the invention and will therefore be transgenic.

[00075] The transgenic plants of the invention may be self-pollinated to provide seeds of homozygous transgenic plants of the invention (homozygous for the recombinant DNA molecule), or crossed with non-transgenic plants or other transgenic plants to provide seeds of the homozygous transgenic plants of the invention (homozygous for the recombinant DNA molecule DNA). Both homozygous and heterozygous transgenic plants are referred to herein as "progeny plants". Descendant plants are transgenic plants derived from the original transgenic plant and containing the recombinant DNA molecule of the invention. Seeds produced using the transgenic plant of the invention can be collected and used to grow generations of transgenic plants, i . progeny plants of the invention, comprising the genetic construct of the present invention and the expression of a gene of agronomic interest. Descriptions of propagation methods commonly used for various crops can be found in one of several book references, see for example Allard, Principles of Plant Breeding , John Wiley & Sons, NY, U. of CA, Davis, CA, 50 -98 (1960); Simmonds, Principles of Crop Improvement , Longman, Inc., NY, 369-399 (1979); Sneep and Hendriksen, Plant breeding Perspectives , Wageningen (ed.), Center for Agricultural Publishing and Documentation (1979); Fehr, Soybeans: Improvement, Production and Uses , ^2nd Edition, Monograph, 16:249 (1987); Fehr, Principles of Variety Development , Theory and Technique, (Vol. 1) and Crop Species Soybean (Vol. 2), Iowa State Univ., Macmillan Pub. Co., NY, 360-376 (1987).

[00076] Transformed plants can be analyzed for the presence of the target gene or genes and for the level of expression and/or profile provided by the regulatory elements of the invention. Those skilled in the art are aware of the numerous methods available for analyzing transformed plants. For example, plant analysis methods include, but are not limited to, Southern blots or Northern blots, PCR-based approaches, biochemical assays, phenotypic screening methods, field trials, and immunodiagnostic assays. Expression of the transcribed DNA molecule can be measured using TaqMan® reagents (Applied Biosystems, Foster City, CA) and by the methods described by the manufacturer and by the number of PCR cycles determined using the TaqMan® Testing Matrix. Alternatively, Invader® reagents (Third Wave Technologies, Madison, WI) and methods described by the manufacturer can be used to evaluate transgene expression.

[00077] The invention also relates to parts of the plant according to the invention. Plant parts include, but are not limited to, leaves, stems, roots, tubers, seeds, endosperm, ovule, and pollen. Parts of plants according to the invention may be viable, non-viable, capable and/or not capable of regeneration. The invention also includes and provides transformed plant cells containing the DNA molecule of the invention. Transformed cells or cells of a transgenic plant according to the invention include cells capable and/or not capable of regeneration.

[00078] The invention may be more readily understood by reference to the following examples, which are provided by way of illustration and are not intended to limit the invention unless stated otherwise. It will be appreciated by those skilled in the art that the methods described in the following examples are the methods found by the inventors to function normally in the practice of the invention. However, it should be clear to those skilled in the art, in light of the present disclosure, that many changes can be made to the specific embodiments of the invention described and still produce a similar or analogous result without departing from the spirit and scope of the invention, so anything of significance is placed hereinafter or shown in the accompanying graphics and should be interpreted in an illustrative and not limiting sense.

EXAMPLES

Example 1

Identification and cloning of regulatory elements

[00079] Novel ubiquitin regulatory elements, or Expression Regulatory Element (EXP) group sequences, have been identified and isolated from the genomic DNA of monocots: dark bentgrass ( Agrostis nebulosa ), cane arundo ( Arundo donax ), graceful buteloui ( Bouteloua gracilis ), Chinese miscanthus ( Miscanthus sinensis ), panicled schizachyrium ( Schizachyrium scoparium ), drooping sorghastrum ( Sorghastrum nutans ) and coli ( Coix lacryma-jobi ).

[00080] Ubiquitin 1 transcript sequences have been identified in each of the above species. The 5'-untranslated region (5'-UTR) of each of the ubiquitin 1 transcripts was used to design primers for the amplification of the corresponding regulatory elements of the identified ubiquitin gene, which includes a promoter, a leader (5'-UTR), and the first intron functionally linked to each other. The primers were used with GenomeWalker ^™ (Clontech Laboratories, Inc, Mountain View, CA) libraries designed according to the manufacturer's protocol to clone the 5' region of the corresponding genomic DNA sequence. Ubiquitin regulatory elements have also been isolated from the monocots Setaria italica , Setaria viridis , and Zea mays subsp. Mexicana (Teosinte) using the GenomeWalker ^™ libraries as described above. In addition, ubiquitin regulatory elements have also been isolated from the monocot Sorghum bicolor using publicly available sequences that are homologues to ubiquitin genes 4, 6, and 7.

[00081] Using the identified sequences, bioinformatic analysis was performed to identify regulatory elements within the amplified DNA. Using the results of this analysis, regulatory elements were identified within the DNA sequences and primers designed to amplify the regulatory elements. The corresponding DNA molecule for each regulatory element was amplified using standard polymerase chain reaction (PCR) conditions with primers containing unique restriction sites and genomic DNA isolated from Aβ . nebulosa, A donax, B. gracilis, M. sinensis, S. scoparium , S. nutans, and C. lacryma-jobi . The resulting DNA fragments were ligated into basic plant expression vectors and sequenced. Then, the transcription initiation site (TSIT) of the regulatory element and the intron/exon of the splicing junction were analyzed using transformed plant protoplasts. Briefly, protoplasts were transformed with plant expression vectors containing cloned DNA fragments operably linked to a heterologous transcribed DNA molecule, and the 5'-RACE system for rapid cDNA end amplification, version 2.0 (Invitrogen, Carlsbad, California 92008) was used to confirm SIT. regulatory element and intron/exon of the splicing junction by analyzing the sequence of messenger RNA (mRNA) transcripts obtained in this way.

[00082] The DNA sequences identified by EXP are provided herein as SEQ ID NOs: 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41 , 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108 , 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150, and 168, which are shown in Table 1 below. No.: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 and 169. Leader sequences are provided herein as SEQ ID NOs: 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152 and 170. Intron sequences provided herein as SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 and 171.

Table 1. Regulatory expression element ("EXP") groups, promoters, enhancers, leaders, and introns isolated from various cereal species.

Description SEQID No.: Size (p. n.) Genus/Species Description and/or regulatory elements from EXP, connected in 5' → 3' direction (SEQ ID no.): EXP-AGRne.Ubq1:1:7 1 3143 A. nebulosa EXP: P-AGRne.Ubq1-1:1:5 (SEQ ID no: 2); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:5 2 2005 A. nebulosa promoter L-AGRne.Ubq1-1:1:1 3 85 A. nebulosa Leader I-AGRne.Ubq1-1:1:3 4 1053 A. nebulosa Intron EXP-AGRne.Ubq1:1:8 5 2137 A. nebulosa EXP: P-AGRne.Ubq1-1:1:4 (SEQ ID no: 6); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:4 6 999 A. nebulosa promoter EXP-AGRne.Ubq1:1:9 7 1900 A. nebulosa EXP: P-AGRne.Ubq1-1:1:6 (SEQ ID no: 8); L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3); I-AGRne.Ubq1-1:1:3 (SEQ ID no: 4) P-AGRne.Ubq1-1:1:6 8 762 A. nebulosa promoter EXP-ARUdo.Ubq1:1:4 9 5068 A.donax EXP: P-ARUdo.Ubq1-1:1:4 (SEQ ID no: 10); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:2 (SEQ ID no: 12) P-ARUdo.Ubq1-1:1:4 10 4114 A.donax promoter L-ARUdo.Ubq1-1:1:1 eleven 85 A.donax Leader I-ARUdo.Ubq1-1:1:2 12 869 A.donax Intron EXP-ARUdo.Ubq1:1:8 13 2969 A.donax EXP: P-ARUdo.Ubq1-1:1:5 (SEQ ID no: 14); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) P-ARUdo.Ubq1-1:1:5 14 2012 A.donax promoter I-ARUdo.Ubq1-1:1:3 15 872 A.donax Intron EXP-ARUdo.Ubq1:1:6 16 1954 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:2 (SEQ ID no: 12) P-ARUdo.Ubq1-1:1:6 17 1000 A.donax promoter EXP-ARUdo.Ubq1:1:9 18 1957 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) EXP-ARUdo.Ubq1:1:12 19 1957 A.donax EXP: P-ARUdo.Ubq1-1:1:6 (SEQ ID no: 17); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:4 (SEQ ID no: 20) I-ARUdo.Ubq1-1:1:4 20 872 A.donax Intron EXP-ARUdo.Ubq1:1:11 21 1712 A.donax EXP: P-ARUdo.Ubq1-1:1:8 (SEQ ID no: 22); L-ARUdo.Ubq1-1:1:1 (SEQ ID NO: 11); I-ARUdo.Ubq1-1:1:3 (SEQ ID NO: 15) P-ARUdo.Ubq1-1:1:8 22 755 A.donax promoter EXP-ARUdo.Ubq2:1:4 23 3276 A.donax EXP: P-ARUdo.Ubq2-1:1:4 (SEQ ID no: 24); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:1 (SEQ ID no: 26) P-ARUdo.Ubq2-1:1:4 24 2033 A.donax promoter L-ARUdo.Ubq2-1:1:1 25 88 A.donax Leader I-ARUdo.Ubq2-1:1:1 26 1155 A.donax Intron EXP-ARUdo.Ubq2:1:8 27 3250 A.donax EXP: P-ARUdo.Ubq2-1:1:6 (SEQ ID no: 28); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:6 28 2004 A.donax promoter I-ARUdo.Ubq2-1:1:2 29 1158 A.donax Intron EXP-ARUdo.Ubq2:1:9 thirty 2247 A.donax EXP: P-ARUdo.Ubq2-1:1:5 (SEQ ID no: no. 31); L-ARUdo.Ubq2-1:1:1 (SEQ ID NO: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:5 31 1001 A.donax promoter EXP-ARUdo.Ubq2:1:10 32 1942 A.donax EXP: P-ARUdo.Ubq2-1:1:7 (SEQ ID no: 33); L-ARUdo.Ubq2-1:1:1 (SEQ ID no: 25); I-ARUdo.Ubq2-1:1:2 (SEQ ID no: 29) P-ARUdo.Ubq2-1:1:7 33 696 A.donax promoter EXP-BOUgr.Ubq1:1:1 34 3511 B. gracilis EXP: P-BOUgr.Ubq1-1:1:2 (SEQ ID no: 35); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:2 (SEQ ID no: 37) P-BOUgr.Ubq1-1:1:2 35 2371 B. gracilis promoter L-BOUgr.Ubq1-1:1:1 36 86 B. gracilis Leader I-BOUgr.Ubq1-1:1:2 37 1054 B. gracilis Intron EXP-BOUgr.Ubq1:1:6 38 3142 B. gracilis EXP: P-BOUgr.Ubq1-1:1:3 (SEQ ID no: 39); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:3 39 1999 B. gracilis promoter I-BOUgr.Ubq1-1:1:3 40 1057 B. gracilis Intron EXP-BOUgr.Ubq1:1:7 41 2165 B. gracilis EXP: P-BOUgr.Ubq1-1:1:5 (SEQ ID no: 42); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:5 42 1022 B. gracilis promoter EXP-BOUgr.Ubq1:1:8 43 1903 B. gracilis EXP: P-BOUgr.Ubq1-1:1:6 (SEQ ID no: 44); L-BOUgr.Ubq1-1:1:1 (SEQ ID no: 36); I-BOUgr.Ubq1-1:1:3 (SEQ ID no: 40) P-BOUgr.Ubq1-1:1:6 44 760 B. gracilis promoter EXP-BOUgr.Ubq2:1:11 45 3234 B. gracilis EXP: P-BOUgr.Ubq2-1:1:4 (SEQ ID no: 46); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:3 (SEQ ID no: 48) P-BOUgr.Ubq2-1:1:4 46 2100 B. gracilis promoter L-BOUgr.Ubq2-1:1:1 47 91 B. gracilis Leader I-BOUgr.Ubq2-1:1:3 48 1043 B. gracilis Intron EXP-BOUgr.Ubq2:1:7 49 3176 B. gracilis EXP: P-BOUgr.Ubq2-1:1:7 (SEQ ID no: 50); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:1 (SEQ ID no: 51) P-BOUgr.Ubq2-1:1:7 50 2043 B. gracilis promoter I-BOUgr.Ubq2-1:1:1 51 1042 B. gracilis Intron EXP-BOUgr.Ubq2:1:14 52 3139 B. gracilis EXP: P-BOUgr.Ubq2-1:1:5 (SEQ ID no: 53); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:4 (SEQ ID no: 54) P-BOUgr.Ubq2-1:1:5 53 2002 B. gracilis promoter I-BOUgr.Ubq2-1:1:4 54 1046 B. gracilis Intron EXP-BOUgr.Ubq2:1:15 55 2160 B. gracilis EXP: P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:5 (SEQ ID no: 57) P-BOUgr.Ubq2-1:1:6 56 1024 B. gracilis promoter I-BOUgr.Ubq2-1:1:5 57 1045 B. gracilis Intron EXP-BOUgr.Ubq2:1:16 58 2160 B. gracilis EXP: P-BOUgr.Ubq2-1:1:6 (SEQ ID no: 56); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:6 (SEQ ID no: 59) I-BOUgr.Ubq2-1:1:6 59 1045 B. gracilis Intron EXP-BOUgr.Ubq2:1:17 60 1885 B. gracilis EXP: P-BOUgr.Ubq2-1:1:8 (SEQ ID no: 61); L-BOUgr.Ubq2-1:1:1 (SEQ ID no: 47); I-BOUgr.Ubq2-1:1:6 (SEQ ID no: 59) P-BOUgr.Ubq2-1:1:8 61 749 B. gracilis promoter EXP-MISsi.Ubq1:1:2 62 6813 M. sinensis EXP: P-MISsi.Ubq1-1:1:2 (SEQ ID no: 63); L-MISsi.Ubq1-1:1:1 (SEQ ID no: 64); I-MISsi.Ubq1-1:1:1 (SEQ ID no: 65) P-MISsi.Ubq1-1:1:2 63 5359 M. sinensis promoter L-MISsi.Ubq1-1:1:1 64 63 M. sinensis Leader I-MISsi.Ubq1-1:1:1 65 1391 M. sinensis Intron EXP-MISsi.Ubq1:1:9 66 4402 M. sinensis EXP: P-MISsi.Ubq1-1:1:11 (SEQ ID no: 67); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:11 67 2423 M. sinensis promoter L-MISsi.Ubq1-1:1:2 68 55 M. sinensis Leader I-MISsi.Ubq1-1:1:3 69 1924 M. sinensis Intron EXP-MISsi.Ubq1:1:8 70 3426 M. sinensis EXP: P-MISsi.Ubq1-1:1:10 (SEQ ID no: 71); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:10 71 1447 M. sinensis promoter EXP-MISsi.Ubq1:1:10 72 2878 M. sinensis EXP: P-MISsi.Ubq1-1:1:13 (SEQ ID no: 73); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:13 73 899 M. sinensis promoter EXP-MISsi.Ubq1:1:11 74 2670 M. sinensis EXP: P-MISsi.Ubq1-1:1:14 (SEQ ID no: 75); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:14 75 691 M. sinensis promoter EXP-MISsi.Ubq1:1:7 76 2485 M. sinensis EXP: P-MISsi.Ubq1-1:1:9 (SEQ ID no: 77); L-MISsi.Ubq1-1:1:2 (SEQ ID no: 68); I-MISsi.Ubq1-1:1:3 (SEQ ID no: 69) P-MISsi.Ubq1-1:1:9 77 506 M. sinensis promoter EXP-SCHsc.Ubq1:1:9, 78 4079 S. scoparium EXP: P-SCHsc.Ubq1-1:1:12 (SEQ ID no: 79); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:12 79 2831 S. scoparium promoter L-SCHsc.Ubq1-1:1:3 80 95 S. scoparium Leader I-SCHsc.Ubq1-1:1:2 81 1153 S. scoparium Intron EXP-SCHsc.Ubq1:1:8 82 3281 S. scoparium EXP: P-SCHsc.Ubq1-1:1:11 (SEQ ID no: 83); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:11 83 2033 S. scoparium promoter EXP-SCHsc.Ubq1:1:7 84 2294 S. scoparium EXP: P-SCHsc.Ubq1-1:1:10 (SEQ ID no: 85); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:10 85 1046 S. scoparium promoter EXP-SCHsc.Ubq1:1:10 86 1795 S. scoparium EXP: P-SCHsc.Ubq1-1:1:14 (SEQ ID no: 87); L-SCHsc.Ubq1-1:1:3 (SEQ ID no: 80); I-SCHsc.Ubq1-1:1:2 (SEQ ID NO: 81) P-SCHsc.Ubq1-1:1:14 87 547 S. scoparium promoter EXP-SORnu.Ubq1:1:2 88 3357 S. nutans EXP: P-SORnu.Ubq1-1:1:4 (SEQ ID no: 89); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:1 (SEQ ID no: 91) P-SORnu.Ubq1-1:1:4 89 2218 S. nutans promoter L-SORnu.Ubq1-1:1:1 90 86 S. nutans Leader I-SORnu.Ubq1-1:1:1 91 1053 S. nutans Intron EXP-SORnu.Ubq1:1:6 92 3106 S. nutans EXP: P-SORnu.Ubq1-1:1:5 (SEQ ID no: 93); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:5 93 1964 S. nutans promoter I-SORnu.Ubq1-1:1:2 94 1056 S. nutans Intron EXP-SORnu.Ubq1:1:7 95 2165 S. nutans EXP: P-SORnu.Ubq1-1:1:6 (SEQ ID no: 96); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:6 96 1023 S. nutans promoter EXP-SORnu.Ubq1:1:8 97 1866 S. nutans EXP: P-SORnu.Ubq1-1:1:7 (SEQ ID no: 98); L-SORnu.Ubq1-1:1:1 (SEQ ID no: 90); I-SORnu.Ubq1-1:1:2 (SEQ ID no: 94) P-SORnu.Ubq1-1:1:7 98 724 S. nutans promoter EXP-SETit.Ubq1:1:10 99 2625 S. italica EXP: P-SETit.Ubq1-1:1:4 (SEQ ID NO: 100); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:3 (SEQ ID NO: 102) P-SETit.Ubq1-1:1:4 100 1492 S. italica promoter L-SETit.Ubq1-1:1:1 101 127 S. italica Leader I-SETit.Ubq1-1:1:3 102 1006 S. italica Intron EXP-SETit.Ubq1:1:5 103 2625 S. italica EXP: P-SETit.Ubq1-1:1:1 (SEQ ID NO: 104); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:1 104 1492 S. italica promoter I-SETit.Ubq1-1:1:2 105 1006 S. italica Intron EXP-SETit.Ubq1:1:7 106 2167 S. italica EXP: P-SETit.Ubq1-1:1:2 (SEQ ID NO: 107); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:2 107 1034 S. italica promoter EXP-SETit.Ubq1:1:6 108 1813 S. italica EXP: P-SETit.Ubq1-1:1:3 (SEQ ID NO: 109); L-SETit.Ubq1-1:1:1 (SEQ ID NO: 101); I-SETit.Ubq1-1:1:2 (SEQ ID NO: 105) P-SETit.Ubq1-1:1:3 109 680 S. italica promoter EXP-Sv.Ubq1:1:7 110 2634 S. viridis EXP: P-Sv.Ubq1-1:1:1 (SEQ ID no: 111); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:1 111 1493 S. viridis promoter L-Sv.Ubq1-1:1:2 112 127 S. viridis Leader I-Sv.Ubq1-1:1:2 113 1014 S. viridis Intron EXP-Sv.Ubq1:1:11 114 2634 S. viridis EXP: P-Sv.Ubq1-1:1:1 (SEQ ID no: 111); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:3 (SEQ ID NO: 115) I-Sv.Ubq1-1:1:3 115 1014 S. viridis Intron EXP-Sv.Ubq1:1:8 116 2176 S. viridis EXP: P-Sv.Ubq1-1:1:2 (SEQ ID no: 117); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:2 117 1035 S. viridis promoter EXP-Sv.Ubq1:1:10 118 1822 S. viridis EXP: P-Sv.Ubq1-1:1:4 (SEQ ID no: 119); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:2 (SEQ ID NO: 113) P-Sv.Ubq1-1:1:4 119 681 S. viridis promoter EXP-Sv.Ubq1:1:12 120 1822 S. viridis EXP: P-Sv.Ubq1-1:1:3 (SEQ ID no: 121); L-Sv.Ubq1-1:1:2 (SEQ ID NO: 112); I-Sv.Ubq1-1:1:3 (SEQ ID NO: 115) P-Sv.Ubq1-1:1:3 121 681 S. viridis promoter EXP-Zm.UbqM1:1:6 (allele-1) 122 1925 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:1 (SEQ ID no: 123); L-Zm.UbqM1-1:1:1 (SEQ ID no: 124); I-Zm.UbqM1-1:1:13 (SEQ ID no: 125) P-Zm.UbqM1-1:1:1 (allele-1) 123 850 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:1 (allele-1) 124 78 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:13 (allele-1) 125 997 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:10 (allele-1) 126 1925 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:1 (SEQ ID no: 123); L-Zm.UbqM1-1:1:1 (SEQ ID no: 124); I-Zm.UbqM1-1:1:17 (SEQ ID no: 127) I-Zm.UbqM1-1:1:17 (allele-1) 127 997 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:7 (allele-2) 128 1974 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:4 (SEQ ID no: 129); L-Zm.UbqM1-1:1:5 (SEQ ID no: 130); I-Zm.UbqM1-1:1:14 (SEQ ID no: 131) P-Zm.UbqM1-1:1:4 (allele-2) 129 887 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:5 (allele-2) 130 77 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:14 (allele-2) 131 1010 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:12 (allele-2) 132 1974 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:4 (SEQ ID no: 129); L-Zm.UbqM1-1:1:5 (SEQ ID no: 130); I-Zm.UbqM1-1:1:19 (SEQ ID no: 133) I-Zm.UbqM1-1:1:19 (allele-2) 133 1010 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:8 (allele-3) 134 2008 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:5 (SEQ ID no: 135); L-Zm.UbqM1-1:1:4 (SEQ ID no: 136); I-Zm.UbqM1-1:1:15 (SEQ ID no: 137) P-Zm.UbqM1-1:1:5 (allele-2) 135 877 Z. mays subsp. Mexicana promoter L-Zm.UbqM1-1:1:4 (allele-2) 136 78 Z. mays subsp. Mexicana Leader I-Zm.UbqM1-1:1:15 (allele-2) 137 1053 Z. mays subsp. Mexicana Intron EXP-Zm.UbqM1:1:11 (allele-3) 138 2008 Z. mays subsp. Mexicana EXP: P-Zm.UbqM1-1:1:5 (SEQ ID no: 135); L-Zm.UbqM1-1:1:4 (SEQ ID no: 136); I-Zm.UbqM1-1:1:18 (SEQ ID no: 139) I-Zm.UbqM1-1:1:18 (allele-2) 139 1053 Z. mays subsp. Mexicana Intron EXP-Sb.Ubq4:1:2 140 1635 S. bicolor EXP: P-Sb.Ubq4-1:1:1 (SEQ ID no: 141); L-Sb.Ubq4-1:1:1 (SEQ ID NO: 142); I-Sb.Ubq4-1:1:2 (SEQ ID NO: 143) P-Sb.Ubq4-1:1:1 141 401 S. bicolor promoter L-Sb.Ubq4-1:1:1 142 154 S. bicolor Leader I-Sb.Ubq4-1:1:2 143 1080 S. bicolor Intron EXP-Sb.Ubq6:1:2 144 2067 S. bicolor EXP: P-Sb.Ubq6-1:1:1 (SEQ ID no: 145); L-Sb.Ubq6-1:1:1 (SEQ ID no: 146); I-Sb.Ubq6-1:1:2 (SEQ ID NO: 147) P-Sb.Ubq6-1:1:1 145 855 S. bicolor promoter L-Sb.Ubq6-1:1:1 146 136 S. bicolor Leader I-Sb.Ubq6-1:1:2 147 1076 S. bicolor Intron EXP-Sb.Ubq6:1:3 148 2067 S. bicolor EXP: P-Sb.Ubq6-1:1:1 (SEQ ID no: 145); L-Sb.Ubq6-1:1:1 (SEQ ID no: 146); I-Sb.Ubq6-1:1:3 (SEQ ID NO: 149) I-Sb.Ubq6-1:1:3 149 1076 S. bicolor Intron EXP-Sb.Ubq7:1:2 150 2003 S. bicolor EXP: P-Sb.Ubq7-1:1:1 (SEQ ID no: 151); L-Sb.Ubq7-1:1:1 (SEQ ID NO: 152); I-Sb.Ubq7-1:1:2 (SEQ ID NO: 153) P-Sb.Ubq7-1:1:1 151 565 S. bicolor promoter L-Sb.Ubq7-1:1:1 152 77 S. bicolor Leader I-Sb.Ubq7-1:1:2 153 1361 S. bicolor Intron EXP-Cl.Ubq10 168 1790 C. lacrima-jobi EXP: P-Cl.UBQ10 (SEQ ID no: 169); L-Cl.UBQ10 (SEQ ID no: 170); I-Cl.UBQ10 (SEQ ID no: 171) P-Cl.Ubq10 169 481 C. lacrima-jobi promoter L-Cl.Ubq10 170 93 C. lacrima-jobi Leader I-Cl.Ubq10 171 1216 C. lacrima-jobi Intron

[00083] As shown in Table 1, for example, the EXP regulatory sequence designated EXP-AGRne.Ubq1:1:7 (SEQ ID NO: 1), with components isolated from A. nebulosa , includes a promoter element P-AGRne.Ubq1- 1:1:5 (SEQ ID no: 2), functionally linked to the 5'-element of the leader L-AGRne.Ubq1-1:1:1 (SEQ ID no: 3), functionally linked to the 5'-element of the intron I- AGRne.Ubq1-1:1:3 (SEQ ID no: 4). Other EXP sequences are related similarly as shown in Table 1.

[00084] As shown in Table 1, Sequence Listing and FIG. 1-8, variants of promoter sequences from A. nebulosa, A donax, B. gracilis, M. sinensis, S. scoparium, and S. nutans have been developed containing truncated promoter fragments, for example, P-AGRne.Ubq1-1:1: 5 (SEQ ID NO:2), P-ARUdo.Ubq1-1:1:4 (SEQ ID NO:10), or other appropriate promoters from other species, and resulted in, for example, P-AGRne.Ubq1-1 :1:4 (SEQ ID no: 6) and P-ARUdo.Ubq1-1:1:5 (SEQ ID no: 14), as well as other promoter fragments.

[00085] Also listed in Table 1 are three allelic variants isolated using the same primers designed to amplify genomic DNA from Z. mays subsp. Mexicana . Allelic variants of the EXP sequences of Z. mays subsp. Mexicana contain DNA sequences that have a certain identity within various regions of other DNA sequences, but insertions, deletions and nucleotide mismatches can also be detected within each promoter, leader and/or intron of each of the EXP sequences. The EXP sequences designated EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122) and EXP-Zm.UbqM1:1:10 (SEQ ID NO: 126) represent the first allele (allele-1) of Z. mays subsp . Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction. The EXP sequences designated EXP-Zm.UbqM1:1:7 (SEQ ID NO: 128) and EXP-Zm.UbqM1:1:12 (SEQ ID NO: 132) represent the second allele (allele-2) of Z. mays subsp . Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction. The EXP sequences EXP-Zm.UbqM1:1:8 (SEQ ID NO: 134) and EXP-Zm.UbqM1:1:11 (SEQ ID NO: 138) represent the third allele (allele-3) of Z. mays subsp. Mexicana in a group of expression regulatory elements with the Ubq1 gene, with the only difference between the two EXP sequences occurring at the last 3'-nucleotides of each respective intron following the 5'-AG-3' sequence of the 3'-intron of the splicing junction.

Example 2

Analysis of regulatory elements controlling GUS in maize protoplasts using GUS

amplicon expression cassettes

[00086] Maize leaf protoplasts were transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the beta-glucuronidase (GUS) transgene and compared with leaf protoplasts in which GUS expression was under the control of known constitutive promoters, in series of experiments below.

[00087] In a first set of experiments, maize cell protoplasts derived from leaf tissue were transformed as previously described with amplicons derived from the amplification of GUS gene expression cassettes containing plant expression vectors to compare transgene (GUS) expression under the control of one from the sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-AGRne.Ubq1:1:9 (SEQ ID no: 7), EXP-ARUdo.Ubq1:1:8 (SEQ ID No.: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID No.: 16), EXP-ARUdo.Ubq1:1:11 (SEQ ID No. : 20), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 26), EXP-ARUdo.Ubq2:1:9 (SEQ ID NO: 29), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 31), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 37), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 40), EXP-BOUgr.Ubq1:1:8 (SEQ ID no: 42), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 51), EXP-BOUgr.Ubq2:1:16 (SEQ ID no: 57), EXP-BOUgr.Ubq2:1:17 ( SEQ ID NO: 59), EXP-MISsi.Ubq1:1:8 (SEQ ID NO: 69), EXP-MISsi.Ubq1:1:10 (SEQ ID NO: 71), EXP-MISsi.Ubq1:1:11 (SEQ ID NO: 73), EXP-MISsi.Ubq1:1:7 (SEQ ID NO: 75), EXP-SCHsc.Ubq1:1:9 (SEQ ID NO: 77), EXP-SCHsc.Ubq1:1: 7 (SEQ ID NO: 83), EXP-SCHsc.Ubq1:1:10 (SEQ ID NO: 85), EXP-SORnu.Ubq1:1:6 (SEQ ID NO: 91), EXP-SORnu.Ubq1:1 :7 (SEQ ID No.: 94), EXP-SORnu.Ubq1:1:8 (SEQ ID No.: 96), EXP-SETit.Ubq1:1:5 (SEQ ID No.: 102), EXP-SETit.Ubq1: 1:7 (SEQ ID No.: 105), EXP-SETit.Ubq1:1:6 (SEQ ID No.: 107), EXP-Sv.Ubq1:1:7 (SEQ ID No.: 109), EXP-Sv.Ubq1 :1:8 (SEQ ID NO: 115), EXP-Sv.Ubq1:1:10 (SEQ ID NO: 117), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 121), EXP-Zm. UbqM1:1:7 (SEQ ID NO: 127), EXP-Zm.UbqM1:1:8 (SEQ ID NO: 133), Exp-Sb.Ubq4:1:2 (SEQ ID NO: 139) and Exp-Sb .Ubq6:1:2 (SEQ ID NO: 143) under the control of known constitutive promoters. Each EXP sequence containing the amplification template from which the amplicon expression cassette is derived was cloned using methods known in the art into the plant expression vector shown in Table 2 below under the heading "amplicon template". The resulting plant expression vectors contain an EXP sequence operably linked to a 5' GUS coding sequence that contains either a processable intron ("GUS-2", SEQ ID no. 154) or an adjacent GUS coding sequence ("GUS-1", SEQ ID no: 153) operably linked to the 5'-3'-UTR of T-AGRtu.nos-1:1:13 (SEQ ID no: 157) or T-Ta.Hsp17-1:1:1 (SEQ ID no: 158). Amplicons were generated using methods known to those skilled in the art using the plasmid construct templates shown in Table 2 below. Briefly, a 5' oligonucleotide primer was designed to anneal the promoter sequence, and a 3' oligonucleotide primer that anneals from the 3' end of the 3' UTR was used to amplify each expression cassette. Sequential 5' deletions were introduced into the promoter sequences constituting the expression cassettes, giving rise to different EXP sequences, by using different oligonucleotide primers that were designed to anneal at different positions within the promoter sequence constituting each amplicon template.

Table 2. Amplicons for expression in plants with the GUS gene sequence and corresponding templates for plasmid amplicon constructs, the EXP sequence encoding the GUS sequence, and the 3'-UTR used to transform maize leaf protoplasts.

Amplicon ID Amplicon matrix EXP follow
validity SEQID No.: GUS coding sequence 3'-UTR PCR0145942 pMON25455 EXP-Os.Act1:1:9 162 GUS-1 T-AGRtu.nos-1:1:13 PCR0145943 pMON65328 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 GUS-2 T-Ta.Hsp17-1:1:1 PCR0145935 pMON140890 EXP-AGRne.Ubq1:1:7 1 GUS-1 T-AGRtu.nos-1:1:13 PCR0145827 pMON140890 EXP-AGRne.Ubq1:1:8 5 GUS-1 T-AGRtu.nos-1:1:13 PCR0145828 pMON140890 EXP-AGRne.Ubq1:1:9 7 GUS-1 T-AGRtu.nos-1:1:13 PCR0145939 pMON140894 EXP-ARUdo.Ubq1:1:8 13 GUS-1 T-AGRtu.nos-1:1:13 PCR0145837 pMON140894 EXP-ARUdo.Ubq1:1:9 18 GUS-1 T-AGRtu.nos-1:1:13 PCR0145838 pMON140894 EXP-ARUdo.Ubq1:1:11 21 GUS-1 T-AGRtu.nos-1:1:13 PCR0145940 pMON140895 EXP-ARUdo.Ubq2:1:8 27 GUS-1 T-AGRtu.nos-1:1:13 PCR0145841 pMON140895 EXP-ARUdo.Ubq2:1:9 thirty GUS-1 T-AGRtu.nos-1:1:13 PCR0145842 pMON140895 EXP-ARUdo.Ubq2:1:10 32 GUS-1 T-AGRtu.nos-1:1:13 PCR0145936 pMON140891 EXP-BOUgr.Ubq1:1:6 38 GUS-1 T-AGRtu.nos-1:1:13 PCR0145829 pMON140891 EXP-BOUgr.Ubq1:1:7 41 GUS-1 T-AGRtu.nos-1:1:13 PCR0145831 pMON140891 EXP-BOUgr.Ubq1:1:8 43 GUS-1 T-AGRtu.nos-1:1:13 PCR0145937 pMON140892 EXP-BOUgr.Ubq2:1:14 52 GUS-1 T-AGRtu.nos-1:1:13 PCR0145833 pMON140892 EXP-BOUgr.Ubq2:1:16 58 GUS-1 T-AGRtu.nos-1:1:13 PCR0145836 pMON140892 EXP-BOUgr.Ubq2:1:17 60 GUS-1 T-AGRtu.nos-1:1:13 PCR0145898 pMON136265 EXP-MISsi.Ubq1:1:8 70 GUS-1 T-AGRtu.nos-1:1:13 PCR0145823 pMON136265 EXP-MISsi.Ubq1:1:10 72 GUS-1 T-AGRtu.nos-1:1:13 PCR0145824 pMON136265 EXP-MISsi.Ubq1:1:11 74 GUS-1 T-AGRtu.nos-1:1:13 PCR0145899 pMON136260 EXP-MISsi.Ubq1:1:7 76 GUS-1 T-AGRtu.nos-1:1:13 PCR0145894 pMON136262 EXP-SCHsc.Ubq1:1:9, 78 GUS-1 T-AGRtu.nos-1:1:13 PCR0145895 pMON136257 EXP-SCHsc.Ubq1:1:7 84 GUS-1 T-AGRtu.nos-1:1:13 PCR0145813 pMON136257 EXP-SCHsc.Ubq1:1:10 86 GUS-1 T-AGRtu.nos-1:1:13 PCR0145938 pMON140893 EXP-SORnu.Ubq1:1:6 92 GUS-1 T-AGRtu.nos-1:1:13 PCR0145839 pMON140893 EXP-SORnu.Ubq1:1:7 95 GUS-1 T-AGRtu.nos-1:1:13 PCR0145840 pMON140893 EXP-SORnu.Ubq1:1:8 97 GUS-1 T-AGRtu.nos-1:1:13 PCR0145900 pMON140877 EXP-SETit.Ubq1:1:5 103 GUS-1 T-AGRtu.nos-1:1:13 PCR0145928 pMON140877 EXP-SETit.Ubq1:1:7 106 GUS-1 T-AGRtu.nos-1:1:13 PCR0145905 pMON140877 EXP-SETit.Ubq1:1:6 108 GUS-1 T-AGRtu.nos-1:1:13 PCR0145909 pMON140878 EXP-Sv.Ubq1:1:7 110 GUS-1 T-AGRtu.nos-1:1:13 PCR0145929 pMON140878 EXP-Sv.Ubq1:1:8 116 GUS-1 T-AGRtu.nos-1:1:13 PCR0145911 pMON140878 EXP-Sv.Ubq1:1:10 118 GUS-1 T-AGRtu.nos-1:1:13 PCR0145914 pMON140881 EXP-Zm.UbqM1:1:6 122 GUS-1 T-AGRtu.nos-1:1:13 PCR0145916 pMON140883 EXP-Zm.UbqM1:1:7 128 GUS-1 T-AGRtu.nos-1:1:13 PCR0145915 pMON140882 EXP-Zm.UbqM1:1:8 134 GUS-1 T-AGRtu.nos-1:1:13 PCR0145921 pMON140887 Exp-Sb.Ubq4:1:2 140 GUS-1 T-AGRtu.nos-1:1:13 PCR0145920 pMON140886 Exp-Sb.Ubq6:1:2 144 GUS-1 T-AGRtu.nos-1:1:13

[00088] The plasmid constructs shown as amplicon templates in Table 2 served as templates for amplifying transgene expression cassettes containing the EXP sequences listed in Table 2. Control plasmids used to generate GUS transgene amplicons for comparison were generated as previously described with constitutive EXP sequences EXP-Os.Act1:1:9 (SEQ ID no: 162) and EXP-CaMV.35S-enh+Ta .Lhcb1+Os.Act1:1:1 (SEQ ID no: 161). An empty vector, not designed to express the transgene, was used as a negative control to assess background expression of GUS and luciferase.

[00089] Two plasmids were also constructed for use in co-transformation and data normalization using methods known in the art. Each plasmid contained a specific luciferase-coding sequence under the control of the constitutive EXP sequence. The plant vector pMON19437 contains an expression cassette with a constitutive promoter operably linked to the 5'-intron (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 163) operably linked to the 5'-coding sequence firefly ( Photinus pyralis ) luciferase (LUCIFERASE:1:3, SEQ ID no: 156) operably linked to the 5'-3'-UTR from the nopaline synthase gene of Agrobacterium tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID No: 158). The plant vector pMON63934 contains an expression cassette with the constitutive sequence EXP (EXP-CaMV.35S-enh-Lhcb1, SEQ ID no: 164) operably linked to the 5'-coding sequence of the sea sponge luciferase ( Renilla reniformis ) (CR-Ren.hRenilla Lucife -0:0:1, SEQ ID no: 157) operably linked to the 5'-3'-UTR from the Agrobacterium tumefaciens nopaline synthase gene (T-AGRtu.nos-1:1:13, SEQ ID no: 158).

[00090] Maize leaf protoplasts were transformed using a PEG-based transformation method that is well known in the art. Protoplast cells were transformed with pMON19437 and pMON63934 plasmid DNA and the amplicons shown in Table 2 and incubated overnight in total darkness. Measurements of both GUS and luciferase were performed by placing aliquots of lysed cell preparations transformed as previously described in two different small well plates. One plate was used to measure GUS and the second plate was used to perform a dual luciferase assay using a dual luciferase reporter assay system (Promega Corp., Madison, WI; see, for example, Promega Notes Magazine, No: 57, 1996, p.02 ). One or two transformations were performed for each EXP sequence and average expression values were determined for each EXP sequence from several samples of each transformation experiment. Sample measurements were performed using four replications for each transformation with the EXP sequence genetic construct, or alternatively three replications for each EXP sequence amplicon in one of two transformation experiments. Values for GUS and luciferase expression levels are shown in Table 3. In this table, firefly luciferase values ( e.g. as a result of expression of pMON19437) are shown in the column labeled "FLuc" and Renilla luciferase values are listed in the column labeled "RLuc".

Table 3 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 5 7840.58 205661 EXP-Os.Act1:1:9 162 1540.25 2671.83 105417 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 12530.8 3067.08 137723 EXP-AGRne.Ubq1:1:7 1 39665 3645.83 137384 EXP-AGRne.Ubq1:1:8 5 22805.5 4183.58 140991 EXP-AGRne.Ubq1:1:9 7 5861.5 887.08 34034.3 EXP-ARUdo.Ubq1:1:8 13 26965.5 1052.33 37774.8 EXP-ARUdo.Ubq1:1:9 18 66126 3251.08 114622 EXP-ARUdo.Ubq1:1:11 21 136163 453851 EXP-ARUdo.Ubq2:1:8 27 13222.3 2203.58 72339.1 EXP-ARUdo.Ubq2:1:9 thirty 30095 6538.58 229201 EXP-ARUdo.Ubq2:1:10 32 16448.5 1842.58 65325.1 EXP-BOUgr.Ubq1:1:6 38 32544.3 2765.08 80330.8 EXP-BOUgr.Ubq1:1:7 41 3826.33 697.11 20709 EXP-BOUgr.Ubq1:1:8 43 9935.5 3372.58 110965 EXP-BOUgr.Ubq2:1:14 52 17828 1575.83 62286.8 EXP-BOUgr.Ubq2:1:16 58 54970.3 3389.08 117616 EXP-BOUgr.Ubq2:1:17 60 48601.3 7139.08 245785 EXP-MISsi.Ubq1:1:8 70 11788.3 3264.58 87751.6 EXP-MISsi.Ubq1:1:10 72 33329.5 2388.58 81000.6 EXP-MISsi.Ubq1:1:11 74 4723.75 3135.33 98059.1 EXP-MISsi.Ubq1:1:7 76 4499 3073.58 84015.1 EXP-SCHsc.Ubq1:1:9, 78 5972 1703.33 62310.6 EXP-SCHsc.Ubq1:1:7 84 24173.5 5306.08 155122 EXP-SCHsc.Ubq1:1:10 86 7260 1171.08 38698.1 EXP-SORnu.Ubq1:1:6 92 3966.5 4175.08 129365 EXP-SORnu.Ubq1:1:7 95 23375.5 616.83 25125.3 EXP-SORnu.Ubq1:1:8 97 8431.75 1630.08 55095.6 EXP-SETit.Ubq1:1:5 103 20496.5 2358.83 88695.8 EXP-SETit.Ubq1:1:7 106 75728.5 4723.08 185224 EXP-SETit.Ubq1:1:6 108 44148.3 4962.08 161216 EXP-Sv.Ubq1:1:7 110 15043.8 1888.33 74670.6 EXP-Sv.Ubq1:1:8 116 31997.8 3219.83 113787 EXP-Sv.Ubq1:1:10 118 38952.8 7011.33 220209 EXP-Zm.UbqM1:1:6 122 30528.3 2453.58 90113.1 EXP-Zm.UbqM1:1:8 134 34986.3 2553.78 105725 Exp-Sb.Ubq4:1:2 140 9982.25 2171.58 72593.8 Exp-Sb.Ubq6:1:2 144 33689 3879.58 114710

[00091] To compare the relative activity of each EXP sequence, GUS values were expressed as the ratio of GUS activity to luciferase activity and normalized to the expression levels observed for EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+ Ta.Lhcb1+Os.Act1:1:1. Table 4 below shows GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 controlled expression in maize protoplasts . Table 5 below shows GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:1 and EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 controlled expression in maize protoplasts .

Table 4. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 (SEQ ID NO: 161) in maize protoplasts.

EXP sequence SEQID No.: GUS/FLuc vs. EXP-CaMV.35S-enh+Ta.Lhcb1+
Os.Act1:1:1 GUS/RLuc vs. EXP-CaMV.35S-enh+Ta.Lhcb1+
Os.Act1:1:1 EXP-Os.Act1:1:9 162 0.14 0.16 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 1 1 EXP-AGRne.Ubq1:1:7 1 2.66 3.17 EXP-AGRne.Ubq1:1:8 5 1.33 1.78 EXP-AGRne.Ubq1:1:9 7 1.62 1.89 EXP-ARUdo.Ubq1:1:8 13 6.27 7.85 EXP-ARUdo.Ubq1:1:9 18 4.98 6.34 EXP-ARUdo.Ubq1:1:11 21 3.3 EXP-ARUdo.Ubq2:1:8 27 1.47 2.01 EXP-ARUdo.Ubq2:1:9 thirty 1.13 1.44 EXP-ARUdo.Ubq2:1:10 32 2.18 2.77 EXP-BOUgr.Ubq1:1:6 38 2.88 4.45 EXP-BOUgr.Ubq1:1:7 41 1.34 2.03 EXP-BOUgr.Ubq1:1:8 43 0.72 0.98 EXP-BOUgr.Ubq2:1:14 52 2.77 3.15 EXP-BOUgr.Ubq2:1:16 58 3.97 5.14 EXP-BOUgr.Ubq2:1:17 60 1.67 2.17 EXP-MISsi.Ubq1:1:8 70 0.88 1.48 EXP-MISsi.Ubq1:1:10 72 3.42 4.52 EXP-MISsi.Ubq1:1:11 74 0.37 0.53 EXP-MISsi.Ubq1:1:7 76 0.36 0.59 EXP-SCHsc.Ubq1:1:9, 78 0.86 1.05 EXP-SCHsc.Ubq1:1:7 84 1.12 1.71 EXP-SCHsc.Ubq1:1:10 86 1.52 2.06 EXP-SORnu.Ubq1:1:6 92 0.23 0.34 EXP-SORnu.Ubq1:1:7 95 9.28 10.23 EXP-SORnu.Ubq1:1:8 97 1.27 1.68 EXP-SETit.Ubq1:1:5 103 2.13 2.54 EXP-SETit.Ubq1:1:7 106 3.92 4.49 EXP-SETit.Ubq1:1:6 108 2.18 3.01 EXP-Sv.Ubq1:1:7 110 1.95 2.21 EXP-Sv.Ubq1:1:8 116 2.43 3.09 EXP-Sv.Ubq1:1:10 118 1.36 1.94 EXP-Zm.UbqM1:1:6 122 3.05 3.72 EXP-Zm.UbqM1:1:8 134 3.35 3.64 Exp-Sb.Ubq4:1:2 140 1.13 1.51 Exp-Sb.Ubq6:1:2 144 2.13 3.23

Table 5. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 162) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS/FLuc vs. EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-Os.Act1:1:9 EXP-Os.Act1:1:9 162 1 1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 7.09 6.23 EXP-AGRne.Ubq1:1:7 1 18.87 19.76 EXP-AGRne.Ubq1:1:8 5 9.46 11.07 EXP-AGRne.Ubq1:1:9 7 11.46 11.79 EXP-ARUdo.Ubq1:1:8 13 44.45 48.86 EXP-ARUdo.Ubq1:1:9 18 35.28 39.48 EXP-ARUdo.Ubq1:1:11 21 20.53 EXP-ARUdo.Ubq2:1:8 27 10.41 12.51 EXP-ARUdo.Ubq2:1:9 thirty 7.98 8.99 EXP-ARUdo.Ubq2:1:10 32 15.49 17.23 EXP-BOUgr.Ubq1:1:6 38 20.42 27.73 EXP-BOUgr.Ubq1:1:7 41 9.52 12.65 EXP-BOUgr.Ubq1:1:8 43 5.11 6.13 EXP-BOUgr.Ubq2:1:14 52 19.63 19.59 EXP-BOUgr.Ubq2:1:16 58 28.14 31.99 EXP-BOUgr.Ubq2:1:17 60 11.81 13.53 EXP-MISsi.Ubq1:1:8 70 6.26 9.19 EXP-MISsi.Ubq1:1:10 72 24.21 28.16 EXP-MISsi.Ubq1:1:11 74 2.61 3.3 EXP-MISsi.Ubq1:1:7 76 2.54 3.67 EXP-SCHsc.Ubq1:1:9, 78 6.08 6.56 EXP-SCHsc.Ubq1:1:7 84 7.9 10.67 EXP-SCHsc.Ubq1:1:10 86 10.75 12.84 EXP-SORnu.Ubq1:1:6 92 1.65 2.1 EXP-SORnu.Ubq1:1:7 95 65.74 63.67 EXP-SORnu.Ubq1:1:8 97 8.97 10.47 EXP-SETit.Ubq1:1:5 103 15.07 15.82 EXP-SETit.Ubq1:1:7 106 27.81 27.98 EXP-SETit.Ubq1:1:6 108 15.43 18.74 EXP-Sv.Ubq1:1:7 110 13.82 13.79 EXP-Sv.Ubq1:1:8 116 17.24 19.25 EXP-Sv.Ubq1:1:10 118 9.64 12.11 EXP-Zm.UbqM1:1:6 122 21.58 23.19 EXP-Zm.UbqM1:1:8 134 23.76 22.65 Exp-Sb.Ubq4:1:2 140 7.97 9.41 Exp-Sb.Ubq6:1:2 144 15.06 20.1

[00092] As can be seen from Tables 9 and 10, all EXP sequences were capable of expressing the GUS transgene in maize cells. The mean GUS expression was higher for all EXP sequences compared to EXP-Os.Act1:1:9. EXP sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-AGRne.Ubq1:1:9 (SEQ ID no: 7), EXP-ARUdo.Ubq1:1:8 (SEQ ID No.: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID No.: 18), EXP-ARUdo.Ubq1:1:11 (SEQ ID No. : 21), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 27), EXP-ARUdo.Ubq2:1:9 (SEQ ID NO: 30), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 32), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:16 (SEQ ID no: 58), EXP-BOUgr.Ubq2:1:17 (SEQ ID no: 60), EXP-MISsi.Ubq1:1:10 ( SEQ ID NO: 72), EXP-SCHsc.Ubq1:1:7 (SEQ ID NO: 84), EXP-SCHsc.Ubq1:1:10 (SEQ ID NO: 86), EXP-SORnu.Ubq1:1:7 (SEQ ID NO: 95), EXP-SORnu.Ubq1:1:8 (SEQ ID NO: 97), EXP-SETit.Ubq1:1:5 (SEQ ID NO: 103), EXP-SETit.Ubq1:1: 7 (SEQ ID NO: 106), EXP-SETit.Ubq1:1:6 (SEQ ID NO: 108), EXP-Sv.Ubq1:1:7 (SEQ ID NO: 110), EXP-Sv.Ubq1:1 :8 (SEQ ID NO: 116), EXP-Sv.Ubq1:1:10 (SEQ ID NO: 118), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122), EXP-Zm.UbqM1: 1:8 (SEQ ID no: 134), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Sb.Ubq6:1:2 (SEQ ID no: 144) showed GUS expression levels higher than than EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1.

[00093] In a second set of experiments, the GUS gene amplicon expression cassette comprising the sequence EXP EXP-Zm.UbqM1:1:7 (SEQ ID NO: 128) was compared with control amplicons, PCR0145942 (EXP-Os.Act1:1:9, SEQ ID no: 162) and PCR0145944 (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 161) in relation to GUS expression. GUS expression under the control of the EXP sequence EXP-Zm.UbqM1:1:7 was higher than in the case of two controls. Table 6 below shows the average GUS and luciferase values determined for each amplicon. Table 7 below shows GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in maize protoplasts .

Table 6 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 1512.25 11333.75 190461.00 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 41176.50 13885.75 330837.25 EXP-Zm.UbqM1:1:7 128 79581.50 15262.50 330755.75

Table 7. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 1512.25 11333.75 190461.00 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 41176.50 13885.75 330837.25 EXP-Zm.UbqM1:1:7 128 79581.50 15262.50 330755.75

[00094] The efficacy of regulatory elements controlling GUS expression from amplicons can be studied in a similar manner in sugar cane leaf protoplasts. For example, sugar cane protoplasts can be transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the GUS transgene and compared to a leaf protoplast in which GUS expression is controlled by known constitutive promoters.

Example 3

Analysis of Regulatory Elements Controlling GUS in Wheat Protoplasts Using GUS Amplicon Expression Cassettes

[00095] Wheat leaf protoplasts were transformed with DNA amplicons derived from plant expression vectors containing the EXP sequence that controls the expression of the GUS transgene and compared to a leaf protoplast in which GUS expression was under the control of known constitutive promoters.

[00096] Wheat protoplast cells derived from leaf tissues were transformed using methods known in the art with amplicons derived from the amplification of GUS gene expression cassettes containing plant expression vectors to compare transgene (GUS) expression under control EXP the sequences listed in Table 3 with that under the control of known constitutive promoters as described in the previous example (Example 2), using the same GUS gene amplicon expression cassettes as used previously for the maize assay in Example 2. Amplicons the control GUS expression cassette and the luciferase gene plasmids used to transform wheat protoplasts were the same as those presented in the previous example and those shown earlier in Table 3 of Example 2. Similarly, negative controls were used to determine the background activity of GUS and luciferase, as described earlier. Wheat leaf protoplasts were transformed using the PEG-based transformation method as previously described in Example 2. Table 8 shows the mean GUS and LUC activity observed in cell protoplasts from transformed wheat leaves, and Tables 9 and 10 show the normalized GUS/FLuc and GUS/RLuc ratios of expression in wheat protoplasts relative to expression under constitutive EXP controls.

Table 8 Mean GUS and luciferase activity in cell protoplasts from transformed wheat leaves.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 262.56 1109.78 61422.1 EXP-Os.Act1:1:9 162 2976.33 730.11 53334.8 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 29299.3 741.78 50717.4 EXP-AGRne.Ubq1:1:7 1 27078.3 754.44 44235.8 EXP-AGRne.Ubq1:1:8 5 22082.7 958.11 55774.8 EXP-AGRne.Ubq1:1:9 7 13882.7 699.78 49273.4 EXP-ARUdo.Ubq1:1:8 13 65628 791.44 56358.8 EXP-ARUdo.Ubq1:1:9 18 87615 801.44 53246.4 EXP-ARUdo.Ubq1:1:11 21 19224.3 143.44 14104.1 EXP-ARUdo.Ubq2:1:8 27 25453.3 835.11 57679.4 EXP-ARUdo.Ubq2:1:9 thirty 26720.7 702.44 47455.4 EXP-ARUdo.Ubq2:1:10 32 37089.3 859.11 57814.4 EXP-BOUgr.Ubq1:1:6 38 35146 995.44 64418.8 EXP-BOUgr.Ubq1:1:7 41 18077 857.78 55793.4 EXP-BOUgr.Ubq1:1:8 43 11723.7 938.44 59362.1 EXP-BOUgr.Ubq2:1:14 52 38109.3 875.11 58048.1 EXP-BOUgr.Ubq2:1:16 58 37384 860.44 52447.8 EXP-BOUgr.Ubq2:1:17 60 24090.7 968.78 53057.8 EXP-MISsi.Ubq1:1:8 70 16456.7 1021.78 61684.1 EXP-MISsi.Ubq1:1:10 72 42816.7 839.78 46688.1 EXP-MISsi.Ubq1:1:11 74 20625.7 987.78 61842.1 EXP-MISsi.Ubq1:1:7 76 4913.67 764.78 64720.1 EXP-SCHsc.Ubq1:1:9, 78 9726 937.11 54725.4 EXP-SCHsc.Ubq1:1:7 84 13374.7 1112.44 73815.4 EXP-SCHsc.Ubq1:1:10 86 13650 936.78 62242.1 EXP-SORnu.Ubq1:1:6 92 8188.17 753.83 50572.5 EXP-SORnu.Ubq1:1:7 95 83233.7 854.44 54410.1 EXP-SORnu.Ubq1:1:8 97 21904.7 1011.83 60852 EXP-SETit.Ubq1:1:5 103 39427.7 908.78 57463.1 EXP-SETit.Ubq1:1:7 106 108091 809.44 49330.4 EXP-SETit.Ubq1:1:6 108 58703 809.11 46110.1 EXP-Sv.Ubq1:1:7 110 29330 684.11 43367.1 EXP-Sv.Ubq1:1:8 116 53359 698.11 40076.4 EXP-Sv.Ubq1:1:10 118 49122.7 901.44 53180.8 EXP-Zm.UbqM1:1:6 122 37268 945.78 54088.1 EXP-Zm.UbqM1:1:8 134 51408 677.78 47297.4 Exp-Sb.Ubq4:1:2 140 35660.3 1114.11 62591.1 Exp-Sb.Ubq6:1:2 144 27543 915.11 57826.4

Table 9. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 (SEQ ID NO: 161) in wheat protoplasts.

EXP sequence SEQID No.: GUS FLuc RLuc Blank vector 262.56 1109.78 61422.1 EXP-Os.Act1:1:9 162 2976.33 730.11 53334.8 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 29299.3 741.78 50717.4 EXP-AGRne.Ubq1:1:7 1 27078.3 754.44 44235.8 EXP-AGRne.Ubq1:1:8 5 22082.7 958.11 55774.8 EXP-AGRne.Ubq1:1:9 7 13882.7 699.78 49273.4 EXP-ARUdo.Ubq1:1:8 13 65628 791.44 56358.8 EXP-ARUdo.Ubq1:1:9 18 87615 801.44 53246.4 EXP-ARUdo.Ubq1:1:11 21 19224.3 143.44 14104.1 EXP-ARUdo.Ubq2:1:8 27 25453.3 835.11 57679.4 EXP-ARUdo.Ubq2:1:9 thirty 26720.7 702.44 47455.4 EXP-ARUdo.Ubq2:1:10 32 37089.3 859.11 57814.4 EXP-BOUgr.Ubq1:1:6 38 35146 995.44 64418.8 EXP-BOUgr.Ubq1:1:7 41 18077 857.78 55793.4 EXP-BOUgr.Ubq1:1:8 43 11723.7 938.44 59362.1 EXP-BOUgr.Ubq2:1:14 52 38109.3 875.11 58048.1 EXP-BOUgr.Ubq2:1:16 58 37384 860.44 52447.8 EXP-BOUgr.Ubq2:1:17 60 24090.7 968.78 53057.8 EXP-MISsi.Ubq1:1:8 70 16456.7 1021.78 61684.1 EXP-MISsi.Ubq1:1:10 72 42816.7 839.78 46688.1 EXP-MISsi.Ubq1:1:11 74 20625.7 987.78 61842.1 EXP-MISsi.Ubq1:1:7 76 4913.67 764.78 64720.1 EXP-SCHsc.Ubq1:1:9, 78 9726 937.11 54725.4 EXP-SCHsc.Ubq1:1:7 84 13374.7 1112.44 73815.4 EXP-SCHsc.Ubq1:1:10 86 13650 936.78 62242.1 EXP-SORnu.Ubq1:1:6 92 8188.17 753.83 50572.5 EXP-SORnu.Ubq1:1:7 95 83233.7 854.44 54410.1 EXP-SORnu.Ubq1:1:8 97 21904.7 1011.83 60852 EXP-SETit.Ubq1:1:5 103 39427.7 908.78 57463.1 EXP-SETit.Ubq1:1:7 106 108091 809.44 49330.4 EXP-SETit.Ubq1:1:6 108 58703 809.11 46110.1 EXP-Sv.Ubq1:1:7 110 29330 684.11 43367.1 EXP-Sv.Ubq1:1:8 116 53359 698.11 40076.4 EXP-Sv.Ubq1:1:10 118 49122.7 901.44 53180.8 EXP-Zm.UbqM1:1:6 122 37268 945.78 54088.1 EXP-Zm.UbqM1:1:8 134 51408 677.78 47297.4 Exp-Sb.Ubq4:1:2 140 35660.3 1114.11 62591.1 Exp-Sb.Ubq6:1:2 144 27543 915.11 57826.4

Table 10. GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 162) in maize leaf protoplasts.

EXP sequence SEQID No.: GUS/FLuc vs. EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-Os.Act1:1:9 EXP-Os.Act1:1:9 162 1 1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 9.69 10.35 EXP-AGRne.Ubq1:1:7 1 8.8 10.97 EXP-AGRne.Ubq1:1:8 5 5.65 7.09 EXP-AGRne.Ubq1:1:9 7 4.87 5.05 EXP-ARUdo.Ubq1:1:8 13 20.34 20.87 EXP-ARUdo.Ubq1:1:9 18 26.82 29.49 EXP-ARUdo.Ubq1:1:11 21 32.88 24.43 EXP-ARUdo.Ubq2:1:8 27 7.48 7.91 EXP-ARUdo.Ubq2:1:9 thirty 9.33 10.09 EXP-ARUdo.Ubq2:1:10 32 10.59 11.5 EXP-BOUgr.Ubq1:1:6 38 8.66 9.78 EXP-BOUgr.Ubq1:1:7 41 5.17 5.81 EXP-BOUgr.Ubq1:1:8 43 3.06 3.54 EXP-BOUgr.Ubq2:1:14 52 10.68 11.76 EXP-BOUgr.Ubq2:1:16 58 10.66 12.77 EXP-BOUgr.Ubq2:1:17 60 6.1 8.14 EXP-MISsi.Ubq1:1:8 70 3.95 4.78 EXP-MISsi.Ubq1:1:10 72 12.51 16.43 EXP-MISsi.Ubq1:1:11 74 5.12 5.98 EXP-MISsi.Ubq1:1:7 76 1.58 1.36 EXP-SCHsc.Ubq1:1:9, 78 2.55 3.18 EXP-SCHsc.Ubq1:1:7 84 2.95 3.25 EXP-SCHsc.Ubq1:1:10 86 3.57 3.93 EXP-SORnu.Ubq1:1:6 92 2.66 2.9 EXP-SORnu.Ubq1:1:7 95 23.9 27.41 EXP-SORnu.Ubq1:1:8 97 5.31 6.45 EXP-SETit.Ubq1:1:5 103 10.64 12.3 EXP-SETit.Ubq1:1:7 106 32.76 39.26 EXP-SETit.Ubq1:1:6 108 17.8 22.81 EXP-Sv.Ubq1:1:7 110 10.52 12.12 EXP-Sv.Ubq1:1:8 116 18.75 23.86 EXP-Sv.Ubq1:1:10 118 13.37 16.55 EXP-Zm.UbqM1:1:6 122 9.67 12.35 EXP-Zm.UbqM1:1:8 134 18.61 19.48 Exp-Sb.Ubq4:1:2 140 7.85 10.21 Exp-Sb.Ubq6:1:2 144 7.38 8.54

[00097] As can be seen from Tables 9 and 10 above, all EXP sequences were capable of expressing the GUS transgene in wheat cells. All EXP sequences expressed GUS at a higher level than EXP-Os.Act1:1:9 in wheat cells. EXP sequences EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP-ARUdo.Ubq1:1:11 (SEQ ID no: 21), EXP-ARUdo.Ubq2:1:10 (SEQ ID no: 32), EXP-BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:16 (SEQ ID no. : 58), EXP-BOUgr.Ubq2:1:17 (SEQ ID no: 60), EXP-MISsi.Ubq1:1:10 (SEQ ID no: 72), EXP-SORnu.Ubq1:1:7 (SEQ ID No.: 95), EXP-SETit.Ubq1:1:5 (SEQ ID No.: 103), EXP-SETit.Ubq1:1:7 (SEQ ID No.: 106), EXP-SETit.Ubq1:1:6 (SEQ ID NO: 108), EXP-Sv.Ubq1:1:7 (SEQ ID NO: 110), EXP-Sv.Ubq1:1:8 (SEQ ID NO: 116), EXP-Sv.Ubq1:1:10 ( SEQ ID NO: 118), EXP-Zm.UbqM1:1:6 (SEQ ID NO: 122) and EXP-Zm.UbqM1:1:8 (SEQ ID NO: 134) showed GUS expression levels equal to or greater than levels of GUS expression under the control of EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 in wheat cells.

[00098] In a second series of experiments, a GUS gene amplicon expression cassette comprising EXP-ARUdo.Ubq1:1:11 (SEQ ID no: 21) was compared with controls EXP-Os.Act1:1:9 (SEQ ID no: 162 ) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID no: 161). Table 11 below shows the average GUS and luciferase values determined for each amplicon. Table 12 below shows GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in wheat protoplasts.

Table 11 Mean GUS and luciferase activity in cell protoplasts from transformed wheat leaves.

EXP sequence SEQID No.: GUS RLuc Blank vector 20.75 187112.50 EXP-Os.Act1:1:9 162 1234.00 176970.50 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 12883.50 119439.00 EXP-ARUdo.Ubq1:1:11 21 30571.50 135037.50

Table 12 GUS/RLuc and GUS/FLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/RLuc in relation to EXP-Os.Act1:1:9 GUS/RLuc in relation to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.
act1:1:1 EXP-Os.Act1:1:9 162 1.00 0.06 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 15.47 1.00 EXP-ARUdo.Ubq1:1:11 21 32.47 2.10

[00099] As can be seen previously from Table 12, GUS expression under the EXP-ARUdo.Ubq1:1:11 control (SEQ ID NO: 21) was higher than the two constitutive controls EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1.

Example 4

Analysis of Regulatory Elements Controlling GUS in Maize and Wheat Protoplasts

[000100] Maize and wheat leaf protoplasts were transformed with plant expression vectors containing an EXP sequence that controls the expression of the beta-glucuronidase (GUS) transgene and compared with GUS expression in leaf protoplasts in which GUS expression was controlled by known constitutive promoters.

[000101] Expression of the transgene under the control of EXP-Cl.Ubq10 (SEQ ID no: 168) was compared with expression under the control of known constitutive promoters. The above EXP sequences were cloned into plant expression vectors as shown in Table 13 below to obtain vectors in which the EXP sequence is operably linked to a 5'-GUS reporter that contained a processable intron (referred to as GUS-2, SEQ ID NO: 160), derived from the potato light-inducible tissue-specific ST-LS1 gene (GenBank Accession: X04753), or an adjacent GUS coding sequence (GUS-1, SEQ ID NO: 159) that has been operably linked to the 5'-3'-UTR from the nopaline synthase gene A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 161) or wheat Hsp17 gene (T-Ta.Hsp17-1:1:1, SEQ ID no: 162).

Table 13 Plant expression plasmid construct with the GUS gene and corresponding EXP sequence encoding the GUS sequence and 3'-UTR used to transform maize leaf protoplasts. "SEQ ID NO:" refers to a given EXP sequence.

EXP sequence SEQID No.: GUS/RLuc in relation to EXP-Os.Act1:1:9 GUS/RLuc vs. EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-Os.Act1:1:9 162 1.00 0.06 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 15.47 1.00 EXP-ARUdo.Ubq1:1:11 21 32.47 2.10

[000102] Two plasmids were also constructed for use in co-transformation and data normalization using methods known in the art. Each plasmid contained a specific luciferase-coding sequence under the control of the constitutive EXP sequence. The plant vector pMON19437 contains an expression cassette with a constitutive promoter operably linked to the 5'-intron (EXP-CaMV.35S-enh+Zm.DnaK:1:1, SEQ ID no: 163) operably linked to the 5'-coding sequence firefly ( Photinus pyralis ) luciferase (LUCIFERASE:1:3, SEQ ID no: 156) operably linked to the 5'-3'-UTR from the nopaline synthase gene of Agrobacterium tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID No: 158). The plant vector pMON63934 contains an expression cassette with the constitutive sequence EXP (EXP-CaMV.35S-enh-Lhcb1, SEQ ID no: 164) operably linked to the 5'-coding sequence of the sea sponge luciferase ( Renilla reniformis ) (CR-Ren.hRenilla Lucife -0:0:1, SEQ ID no: 157) operably linked to the 5'-3'-UTR from the Agrobacterium tumefaciens nopaline synthase gene (T-AGRtu.nos-1:1:13, SEQ ID no: 158).

[000103] Maize leaf protoplasts were transformed using a PEG-based transformation method that is well known in the art. Protoplast cells were transformed with pMON19437 and pMON63934 plasmid DNA and the plasmids shown in Table 13 and incubated overnight in total darkness. Both GUS and luciferase measurements were performed in a similar manner as described in Example 2 previously. One or two transformations were performed for each EXP sequence and average expression values were determined for each EXP sequence from several samples of each transformation experiment. Sample measurements were performed using four replications for each transformation with the EXP sequence genetic construct, or alternatively three replications for each EXP sequence genetic construct in one of two transformation experiments. Values for GUS and luciferase expression levels are shown in Table 14. In this table, firefly luciferase values ( e.g. as a result of expression of pMON19437) are shown in the column labeled "FLuc" and Renilla luciferase values are listed in the column labeled "RLuc".

Table 14 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-Os.Act1:1:9 162 83997.3 80983 61619 EXP-CaMV.35S-
enh+Ta.Lhcb1+Os.Act1:1:1 161 248832 83589.8 72064.3 EXP-Cl.Ubq10 168 30790.8 65807.5 34846.3

[000104] Table 15 below shows GUS/FLuc and GUS/RLuc expression ratios normalized to expression under control of EXP-Os.Act1:1:9 and EXP-CaMV.35S-enh+Zm.DnaK:1:1, in maize protoplasts.

Table 15 GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-Os.Act1:1:9 (SEQ ID NO: 161) and EXP-CaMV.35S-enh+Zm.DnaK:1:1 ( SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/Fluc normalized with respect to EXP-Os.Act1:1:9 GUS/Rluc normalized with respect to EXP-Os.Act1:1:9 GUS/Fluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 GUS/Rluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-Os.Act1:1:9 162 1.00 1.00 0.35 0.39 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 2.87 2.53 1.00 1.00 EXP-Cl.Ubq10 168 0.45 0.65 0.16 0.26

[000105] As can be seen from Table 15 above, EXP-Cl.Ubq10 (SEQ ID NO: 168) was able to express GUS, but at a lower level than both constitutive controls.

[000106] The plasmids shown earlier in Table 13 were also used to transform wheat leaf protoplast cells in a similar manner to the corn leaf protoplasts described previously. Mean GUS and luciferase values are presented in Table 16 below. Table 17 below shows GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-CaMV.35S-enh+Zm.DnaK:1:1 controlled expression in maize protoplasts.

Table 16 Mean GUS and luciferase activity in cell protoplasts from transformed maize leaves.

EXP sequence SEQID No.: GUS FLuc RLuc EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 134145 1076.67 6858.67 EXP-Cl.Ubq10 168 104669 888.67 4516

Table 17. GUS/FLuc and GUS/RLuc expression ratios normalized to EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID NO: 160) in wheat leaf protoplasts.

EXP sequence SEQID No.: GUS/Fluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 GUS/Rluc normalized to EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 161 1.00 1.00 EXP-Cl.Ubq10 168 0.95 1.19

[000107] As can be seen from Table 17 above, EXP-Cl.Ubq10 (SEQ ID NO: 168) expressed GUS at a similar level as EXP-CaMV.35S-enh+Zm.DnaK:1:1 (SEQ ID NO: : 160) in wheat protoplast cells.

Example 5

Analysis of regulatory elements controlling GUS in transgenic maize .

[000108] Corn plants were transformed with plant expression vectors containing EXP sequences that control the expression of the GUS transgene, and the resulting plants were analyzed for GUS protein expression. The ubiquitin EXP sequences were cloned into plant binary transformation plasmid constructs using methods known in the art.

[000109] The resulting plant expression vectors contain the right border region from A. tumefaciens , the first expression cassette for analysis of the EXP sequence operably linked to the GUS coding sequence, which has the processable GUS-2 intron described previously, operably linked to 5'-3' -UTR from rice lipid transfer protein gene (T-Os.LTP-1:1:1, SEQ ID no: 159); a second transgene selection cassette, used to select transformed plant cells, confers resistance to the herbicide glyphosate (under the control of the rice actin 1 promoter) and the left border region from A. tumefaciens . The resulting plasmids were used to transform maize plants. Table 18 lists the plasmid designations, EXP sequences, and SEQ ID Nos., which are also described in Table 1.

Table 18. Binary plasmids for plant transformation and associated EXP sequences.

Plasmid construct EXP sequence SEQID No.: pMON140869 EXP-AGRne.Ubq1:1:7 1 pMON140870 EXP-AGRne.Ubq1:1:8 5 pMON142650 EXP-ARUdo.Ubq1:1:8 13 pMON142651 EXP-ARUdo.Ubq1:1:9 18 pMON142652 EXP-ARUdo.Ubq2:1:8 27 pMON142653 EXP-ARUdo.Ubq2:1:9 thirty pMON140871 EXP-BOUgr.Ubq1:1:6 38 pMON140872 EXP-BOUgr.Ubq1:1:7 41 pMON140873 EXP-BOUgr.Ubq2:1:14 52 pMON140874 EXP-BOUgr.Ubq2:1:15 55 pMON142887 EXP-MISsi.Ubq1:1:7 76 pMON140875 EXP-SORnu.Ubq1:1:6 92 pMON140876 EXP-SORnu.Ubq1:1:7 95 pMON132037 EXP-SETit.Ubq1:1:10 99 pMON131958 EXP-Sv.Ubq1:1:11 114 pMON131959 EXP-Sv.Ubq1:1:12 120 pMON131961 EXP-Zm.UbqM1:1:10 126 pMON131963 EXP-Zm.UbqM1:1:12 132 pMON131962 EXP-Zm.UbqM1:1:11 138 pMON132932 EXP-Sb.Ubq4:1:2 140 pMON132931 EXP-Sb.Ubq6:1:3 148 pMON132974 EXP-Sb.Ubq7:1:2 150 pMON142738 EXP-Cl.Ubq10 168

[000110] Plants were transformed by Agrobacterium- mediated transformation, for example, as described in US Patent Application Publication 2009/0138985.

[000111] GUS histochemical analysis was used to qualitatively analyze the expression of transformed plants. Whole tissue sections were incubated with GUS staining solution X-Gluc (5-bromo-4-chloro-3-indolyl-beta-glucuronide) (1 mg/ml) for an appropriate period of time, washed and checked visually for blue staining. GUS activity was qualitatively determined by direct visual or microscopic examination using selected plant organs and tissues. R ₀ plants were tested for expression in roots and leaves, as well as anther, silk and developing seeds and ovary, 21 days after pollination (21 DPO).

[000112] For quantitative analysis, total protein was extracted from selected tissues of transformed maize plants. One microgram of total protein was used with the fluorogenic substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) in a total reaction volume of 50 μl. The reaction product, 4-methylumbelliferone (4-MU), fluoresces to the maximum at high pH values, where the hydroxyl group is ionized. The addition of an alkaline sodium carbonate solution simultaneously stops the reaction and adjusts the pH to quantify the fluorescent product. Fluorescence was measured at an excitation wavelength of 365 nm, emission at 445 nm using a Fluoromax-3 (Horiba; Kyoto, Japan) with a Micromax Reader, with the slit width set to 2 nm for excitation and 3 nm for emission.

[000113] The average expression of GUS in R ₀ observed for each transformation is presented in tables 19 and 20 below.

Table 19 Average GUS expression in R ₀ in root and leaf tissues.

EXP sequence SEQID No.: V3 root V4 root V7 root VT root V3 sheet V4 sheet V7 sheet VT sheet EXP-AGRne.Ubq1:1:7 1 16 25 14 49 60 48 EXP-AGRne.Ubq1:1:8 5 13 20 22 38 38 52 EXP-ARUdo.Ubq1:1:8 13 18 34 89 117 48 106 EXP-ARUdo.Ubq1:1:9 18 19 20 68 105 33 69 EXP-ARUdo.Ubq2:1:8 27 14 19 27 58 57 47 EXP-ARUdo.Ubq2:1:9 thirty 14 15 25 40 38 40 EXP-BOUgr.Ubq1:1:6 38 12 28 16 43 46 27 EXP-BOUgr.Ubq1:1:7 41 14 24 114 51 48 48 EXP-BOUgr.Ubq2:1:14 52 17 13 28 46 33 41 EXP-BOUgr.Ubq2:1:15 55 eleven 67 36 86 72 36 EXP-MISsi.Ubq1:1:7 76 17 28 13 18 12 18 EXP-SORnu.Ubq1:1:6 92 14 45 33 44 64 55 EXP-SORnu.Ubq1:1:7 95 eleven 18 20 31 36 48 EXP-SETit.Ubq1:1:10 99 0 29 57 58 37 46 EXP-Sv.Ubq1:1:11 114 nd nd 9 20 55 29 EXP-Sv.Ubq1:1:12 120 63 0 28 184 27 16 EXP-Zm.UbqM1:1:10 126 0 237 18 221 272 272 EXP-Zm.UbqM1:1:12 132 0 21 43 234 231 196 EXP-Zm.UbqM1:1:11 138 124 103 112 311 369 297 EXP-Sb.Ubq4:1:2 140 125 0 95 233 150 88 EXP-Sb.Ubq6:1:3 148 154 13 128 53 39 55 EXP-Sb.Ubq7:1:2 150 37 22 18 165 89 177 EXP-Cl.Ubq10 168 61 67 32 111 58 115

Table 20. Average expression of GUS in R ₀ in reproductive organs of corn (anther, silk) and developing seeds (ovary and endosperm).

EXP sequence SEQID No.: VT boot VT/R1 silk 21 dpo ovary 21 dpo endosperm EXP-AGRne.Ubq1:1:7 1 149 36 59 59 EXP-AGRne.Ubq1:1:8 5 73 66 33 58 EXP-ARUdo.Ubq1:1:8 13 321 253 177 355 EXP-ARUdo.Ubq1:1:9 18 242 268 97 266 EXP-ARUdo.Ubq2:1:8 27 104 99 79 157 EXP-ARUdo.Ubq2:1:9 thirty 78 71 82 139 EXP-BOUgr.Ubq1:1:6 38 58 250 43 63 EXP-BOUgr.Ubq1:1:7 41 58 77 40 49 EXP-BOUgr.Ubq2:1:14 52 236 377 48 137 EXP-BOUgr.Ubq2:1:15 55 203 134 47 180 EXP-MISsi.Ubq1:1:7 76 24 16 29 32 EXP-SORnu.Ubq1:1:6 92 361 80 37 94 EXP-SORnu.Ubq1:1:7 95 195 114 20 55 EXP-SETit.Ubq1:1:10 99 132 85 50 63 EXP-Sv.Ubq1:1:11 114 217 3 45 92 EXP-Sv.Ubq1:1:12 120 120 21 49 112 EXP-Zm.UbqM1:1:10 126 261 506 403 376 EXP-Zm.UbqM1:1:12 132 775 362 253 247 EXP-Zm.UbqM1:1:11 138 551 452 234 302 EXP-Sb.Ubq4:1:2 140 213 0 25 79 EXP-Sb.Ubq6:1:3 148 295 87 51 61 EXP-Sb.Ubq7:1:2 150 423 229 274 90 EXP-Cl.Ubq10 168 237 82 91 210

[000114] In R ₀ maize, leaf and root GUS expression levels differed among ubiquitin EXP sequences. Although all EXP sequences showed the ability to control GUS transgene expression in stably transformed plants, each EXP sequence showed a unique expression pattern compared to the others. For example, EXP sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP-ARUdo.Ubq2:1:8 (SEQ ID no: 27), EXP-ARUdo.Ubq2:1:9 (SEQ ID no: 30), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-BOUgr.Ubq2:1:14 ( SEQ ID no: 52), EXP-BOUgr.Ubq2:1:15 (SEQ ID no: 55), EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76), EXP-SORnu.Ubq1:1:6 (SEQ ID NO: 92), EXP-SORnu.Ubq1:1:7 (SEQ ID NO: 95), EXP-SETit.Ubq1:1:10 (SEQ ID NO: 99), EXP-Sv.Ubq1:1: 11 (SEQ ID NO: 114), EXP-Zm.UbqM1:1:12 (SEQ ID NO: 132) and EXP-Sb.Ubq7:1:2 (SEQ ID NO: 150) showed lower levels of GUS expression in the root at V3 and V7 stages of development in relation to EXP-Sv.Ubq1:1:12 (SEQ ID no: 120), EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1 :11 (SEQ ID no: 138), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Sb.Ubq6:1:3 (SEQ ID no: 148). Higher levels of GUS expression were observed at later stages of root development (VT) for EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP -BOUgr.Ubq1:1:7 (SEQ ID NO: 41), EXP-Zm.UbqM1:1:11 (SEQ ID NO: 138) and EXP-Sb.Ubq6:1:3 (SEQ ID NO: 148). Expression in roots under the control of EXP-Zm. UbqM1:1:10 (SEQ ID no: 140) showed no expression at stage V3 but was high at stage V7 and then decreased at VT stage. Expression in roots under the control of EXP-Zm.UbqM1:1:11 (SEQ ID no: 150) was maintained at a similar level throughout development from stages V3 and V7 up to VT. Expression of GUS under the control of EXP-Cl.Ubq10 (SEQ ID NO: 168) was relatively stable from V4 to V7, but decreased by about half in VT as compared to V4 and V7.

[000115] GUS expression levels also showed significant differences in leaf tissue. EXP sequences EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), and EXP-Zm.UbqM1:1:11 (SEQ ID no: 138) showed the highest level of GUS expression observed at all three developmental stages (V3, V7 and VT). The EXP sequence EXP-Sb.Ubq4:1:2 (SEQ ID NO: 140) showed reduced expression at developmental stages V3 to VT. EXP sequences EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13) and EXP-Sb.Ubq7:1:2 (SEQ ID no: 150) showed higher levels of GUS expression at V3 and VT developmental stages, with more low level of expression in the middle of growth at the V7 stage of development. EXP sequence EXP-ARUdo.Ubq2:1:9 (SEQ ID no: 30), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41) and EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76) maintained GUS expression throughout all three steps, while EXP-ARUdo.Ubq2:1:8 (SEQ ID no: 27), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38) and EXP -BOUgr.Ubq2:1:15 (SEQ ID no: 55) showed a slight decrease in expression at the VT stage. Expression under the control of EXP-Cl.Ubq10 (SEQ ID no: 168) was similar at V4 and VT stages, but the level was reduced by about half at V7 stage compared with stages V4 and VT.

[000116] Similarly, with respect to the reproductive tissue (anther and silk), a different expression pattern was observed, unique to each EXP sequence. For example, high expression levels were observed in anther and silk for the EXP sequences: EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1:9 (SEQ ID no: 18), EXP- BOUgr.Ubq2:1:14 (SEQ ID no: 52), EXP-BOUgr.Ubq2:1:15 (SEQ ID no: 55), EXP-SORnu.Ubq1:1:7 (SEQ ID no: 95), EXP -Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), EXP-Zm.UbqM1:1:11 (SEQ ID no: 138), and EXP-Sb.Ubq7:1:2 (SEQ ID NO: 150). EXP-controlled expression of sequences EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-SORnu.Ubq1:1:6 (SEQ ID no: 92), EXP-Sv.Ubq1:1:11 (SEQ ID no: 114), EXP-Sv.Ubq1:1:12 (SEQ ID no: 120), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140), EXP-Sb.Ubq6:1:3 ( SEQ ID no: 148) and EXP-Cl.Ubq10 (SEQ ID no: 168) was high in anther but lower in silk for each EXP sequence, while EXP-BOUgr.Ubq1:1:6 was under control ( SEQ ID NO: 38) was higher in silk than in anther.

[000117] Expression in developing seeds (21 DPO ovary and endosperm) differed among EXP sequences. EXP sequences EXP-Zm.UbqM1:1:10 (SEQ ID no: 126), EXP-Zm.UbqM1:1:12 (SEQ ID no: 132), and EXP-Zm.UbqM1:1:11 (SEQ ID no: 138) carried out a high expression of GUS in the tissues of the ovary and endosperm of developing seeds. Expression levels in the endosperm were about twice or more higher than in the ovary when GUS was under the control of the sequences EXP: EXP-ARUdo.Ubq1:1:8 (SEQ ID no: 13), EXP-ARUdo.Ubq1:1: 9 (SEQ ID NO: 18), EXP-ARUdo.Ubq2:1:8 (SEQ ID NO: 27), EXP-BOUgr.Ubq2:1:14 (SEQ ID NO: 52), EXP-BOUgr.Ubq2:1 :15 (SEQ ID no: 55), EXP-SORnu.Ubq1:1:6 (SEQ ID no: 92), EXP-SORnu.Ubq1:1:7 (SEQ ID no: 95), EXP-Sv.Ubq1: 1:12 (SEQ ID no: 120), EXP-Sb.Ubq4:1:2 (SEQ ID no: 140) and EXP-Cl.Ubq10 (SEQ ID no: 168). GUS expression was three times higher in the ovary than in the endosperm under the control of EXP-Sb.Ubq7:1:2 (SEQ ID no: 150). GUS expression levels were relatively equivalent in ovary and endosperm when controlled by the EXP sequences: EXP-AGRne.Ubq1:1:7 (SEQ ID no: 1), EXP-AGRne.Ubq1:1:8 (SEQ ID no: 5), EXP-BOUgr.Ubq1:1:6 (SEQ ID no: 38), EXP-BOUgr.Ubq1:1:7 (SEQ ID no: 41), EXP-MISsi.Ubq1:1:7 (SEQ ID no: 76) , EXP-SETit.Ubq1:1:10 (SEQ ID NO: 99) and EXP-Sb.Ubq6:1:3 (SEQ ID NO: 148).

[000118] Each EXP sequence showed the ability to control transgene expression in stably transformed maize plants. However, each EXP sequence had a unique expression pattern for each tissue, making it possible to select the EXP sequence that best expresses a particular transgene, depending on the tissue expression strategy required to achieve the desired results. This example shows that EXP sequences isolated from homologous genes do not necessarily behave equivalently in a transformed plant, and that expression can only be determined by empirical studies of the properties of each EXP sequence and cannot be predicted based on the homology of the gene from which the promoter is was received.

Example 6

Enhancers derived from regulatory elements.

[000119] Enhancers are derived from the promoter elements provided herein, such as those shown in SEQ ID NOs: 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 and 169. The enhancer element may contain one or more cis regulatory elements that, when operably linked at the 5' or 3' end to a promoter element, or operably linked at the 5' or 3' end to additional enhancer elements that are operably linked to the promoter, can increase or modulate expression the transgene or to allow the expression of the transgene in a particular cell type or plant organ, or at a particular time point in development or circadian rhythm. Enhancers are created by removing the TATA box or functionally similar elements and any underlying DNA sequence from the promoters, allowing transcription to be initiated from the promoters described herein as described above, including fragments thereof that have the TATA box or functionally similar elements removed. elements and DNA sequence below the TATA box.

[000120] Enhancer elements can be derived from the promoter elements described herein and cloned using methods known in the art such that they are operably linked via the 5' or 3' end to the promoter element or operably linked through the 5' or 3' end with additional enhancer elements that are operably linked to the promoter. Alternatively, enhancer elements are cloned using methods known in the art such that they are operably linked to one or more copies of an enhancer element that is operably linked via the 5' or 3' end to a promoter element or operably linked via 5 ' or 3' end with additional enhancer elements that are operably linked to the promoter. Enhancer elements can also be cloned so that they are operably linked at the 5' or 3' end to a promoter element derived from an organism of another species, or operably linked at the 5' or 3' end to additional enhancer elements derived from organisms of another species. of a genus or an organism of the same genus that is operably linked to a promoter derived from an organism of the same or another genus, resulting in a chimeric regulatory element. The GUS gene expression vector for plant transformation was generated using methods known in the art, similar to the genetic constructs described in the previous examples, in which the resulting plant expression vectors contain the right border region from A. tumefaciens , the first expression cassette for regulatory testing. or a chimeric regulatory element comprising a regulatory or chimeric regulatory element operably linked to an intron derived from the heat shock protein HSP70 from Z. mays (I-Zm.DnaK-1:1:1 SEQ ID no: 165) or any of the introns provided herein, or any other intron operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID no: 155) or no intron (GUS-1, SEQ ID no: 154), operably associated with the 3'-UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 158) or with the 3'-UTR of the rice lipid transfer protein gene (T-Os.LTP-1 :1:1, SEQ ID NO: 160); the second transgene selection cassette, used to select transformed plant cells, confers resistance to the herbicide glyphosate (under the control of the rice actin 1 promoter) or alternatively the antibiotic kanamycin (under the control of the rice actin 1 promoter) and the left boundary region from A. tumefaciens . The resulting plasmids are used to transform corn plants or other plants of this genus using the methods described previously, or other Agrobacterium -mediated transformation or microparticle bombardment methods known in the art. Alternatively, protoplast cells derived from corn or other plants of the genus are transformed using methods known in the art to perform transient expression analysis.

[000121] Expression of the GUS gene under the control of a regulatory element containing one or more enhancers is evaluated in stable transformation or transient expression plants to determine the effect of the enhancer element on transgene expression. Modifications of one or more enhancer elements or duplication of one or more enhancer elements are based on empirical studies and the resulting regulation of gene expression observed using the composition of each regulatory element. Changing the relative positions of one or more enhancers in the final regulatory or chimeric regulatory element can affect the transcriptional activity or specificity of the regulatory or chimeric regulatory element and is determined empirically to determine the best enhancers for the desired transgene expression profile within a corn plant or plant of another genus.

Example 7

Analysis of intron amplification of GUS activity using protoplasts of plant origin.

[000122] The intron is selected based on experimentation and comparison with the expression of a control intronless vector to empirically select the intron and configuration within the location of the DNA transfer elements (T-DNA) of the vector for optimal expression of the transgene. For example, when expressing a herbicide resistance gene such as CP4 that confers glyphosate tolerance, it is desirable to obtain expression of the transgene in reproductive tissues as well as vegetative tissues to prevent crop loss after herbicide application. The intron operably linked to the constitutive promoter will in this case be selected for its ability to enhance the expression of herbicide resistance conferred by the transgene, in particular in the reproductive cells and tissues of the transgenic plant and thus confer both vegetative and reproductive tolerance of the transgenic plant. when sprayed with herbicide. In most ubiquitin genes, the 5'-UTR consists of a leader that has an intron sequence inserted into it. Therefore, regulatory elements derived from such genes were analyzed using the entire 5'-UTR containing promoter, leader and intron. To achieve different expression profiles or modulate the expression level of a transgene, an intron from such a regulatory element can be removed or replaced with a heterologous intron.

[000123] The introns represented herein in SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171 were identified using genomic DNA contigs versus expressed clusters of tag sequences or cDNA contigs to identify exon and intron sequences within the genomic DNA. In addition, 5'-UTRs or leader sequences are also used to define intron/exon splicing junctions of one or more introns under conditions where the gene sequence encodes a leader sequence that is interrupted by one or more introns. The introns were cloned using methods known in the art into a plant transformation vector such that they are operably linked to the 3' regulatory element and the leader fragment and operably linked to the 5' of either the second leader fragment or coding sequences, for example, as shown in the expression cassettes shown in FIG. 9.

[000124] Thus, for example, the first possible expression cassette (expression cassette configuration 1 in Fig. 9) consists of a promoter or chimeric promoter element [A] operably linked to the 5' leader element [B] operably linked to the 5'- test intronic element [C] operably linked to a coding region [D] that is operably linked to the 3'-UTR element [E]. Alternatively, the second possible expression cassette (expression cassette configuration 2 in Figure 9) consists of a promoter or chimeric promoter element [F] operably linked to a 5' first leader element or fragment of the first leader element [G] operably linked to 5'-tested intron element [H] operably linked to the 5'-second leader element or second fragment of the first leader element [I] operably linked to the coding region [J] that is operably linked to the 3'-UTR element [K] . Additionally, a third possible expression cassette (expression cassette configuration 3 in FIG. 9) consists of a promoter or chimeric promoter element [L] operably linked to the 5' leader element [M] operably linked to the 5' first fragment of the coding sequence element [N], operably linked to the 5'-intron element [O], operably linked to the 5'-second fragment of the element of the coding sequence [P], which is operably linked to the 3'-UTR element [Q]. The expression cassette 3 is designed to splice the intron in such a way as to obtain a full open reading frame with no frameshift between the first and second fragment of the coding sequence.

[000125] As previously discussed, it may be preferable to avoid using an AT nucleotide sequence or an A nucleotide sequence immediately before the 5' end of the splice site (GT) and a G nucleotide or nucleotide sequence TG, respectively, immediately after the 3' end of the splicing site (AG) , to eliminate potential unwanted start codons generated during the processing of the messenger RNA into the final transcript. The DNA sequence near the 5' or 3' end of the splicing sites of the intron can thus be modified.

[000126] Introns were tested for the effect of amplification by the ability to enhance expression in the analysis of transient expression or stable expression in the plant. To analyze the intron amplification of transient expression, a basic plant vector was constructed using methods known in the art. The intron was cloned into a basic plant vector that contains an expression cassette consisting of a constitutive promoter such as the cauliflower mosaic virus promoter P-CaMV.35S-enh-1:1:9 (SEQ ID NO: 166) operably linked to 5' -leader element L-CaMV.35S-1:1:15 (SEQ ID no: 167) operably linked to the 5' test intron element ( for example , one of SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171) operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID NO: 155) or lacks an intron (GUS-1, SEQ ID no: 154) operably linked to the 3'UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID no: 158). Protoplast cells derived from maize or other genus plant tissue were transformed with the plant base vector and luciferase gene control vectors as previously described in Example 2 above and analyzed for activity. To compare the relative ability of an intron to enhance expression, GUS values were expressed as the ratio of GUS activity to luciferase activity and compared with those obtained with a genetic construct containing a constitutive promoter operably linked to a known intron standard, such as an intron derived from the HSP70 protein. heat shock Zea mays , I-Zm.DnaK-1:1:1 (SEQ ID no: 165), as well as a genetic construct containing a constitutive promoter, but without an intron functionally associated with the promoter.

[000127] For analysis in stably transformed plants of introns shown in SEQ ID nos: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171, a GUS expression vector for plant transformation similar to the genetic constructs described in the previous examples was developed, in which the resulting plant expression vectors contain the right border region from A. tumefaciens, the first expression cassette for intron testing, consisting of a constitutive promoter , such as the cauliflower mosaic virus promoter P-CaMV.35S-enh-1:1:9 (SEQ ID NO: 166) operably linked to the 5' leader element of L-CaMV.35S-1:1:15 (SEQ ID no: 167) operably linked to the 5' test intron element provided herein operably linked to a GUS coding sequence that either has a processable intron (GUS-2, SEQ ID no: 155) or no intron (GUS -1, SEQ ID NO: 154) operably linked to the 3'UTR of nopaline synthase from A. tumefaciens (T-AGRtu.nos-1:1:13, SEQ ID NO: 158); a second transgene selection cassette, used to select transformed plant cells, confers resistance to glyphosate (under the control of the rice actin 1 promoter) or alternatively the antibiotic kanamycin (under the control of the rice actin 1 promoter) and the left border region from A. tumefaciens . The resulting plasmids are used to transform maize plants or other plants of this genus using the methods described previously, or Agrobacterium -mediated methods known in the art. Single copy or low copy transformants are selected for comparison with single copy or low copy transformed plants transformed with a plant transformation vector identical to the test vector but without the test intron to determine if the test intron provides an intron-mediated enhancement effect.

[000128] Any of the introns shown in SEQ ID NOs: 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 and 171 can be modified in several ways, such as deletion of fragments within the intron sequence, which can reduce expression, or duplication of fragments with an intron, which can increase expression. In addition, DNA sequences within an intron that may affect the specificity of expression, either in particular cell types or in tissues and organs, may be duplicated or altered or deleted in order to affect the expression and expression patterns of the transgene. In addition, the introns provided herein can be modified to remove any potential start codons (ATGs) that could cause unintended transcripts to be expressed from mis-spliced introns as different, extended, or shortened proteins. Once the intron has been empirically verified or altered based on the study, the intron is used to enhance the expression of the transgene in stably transformed plants, which may be monocots or dicots of any genus, provided that the intron enhances the transgene. An intron can also be used to enhance expression in other organisms, such as algae, fungi, or animal cells, provided that the intron provides increased or decreased, or specific expression of the transgene to which it is operatively linked.

[000129] It will be apparent to those skilled in the art from the illustrations and description of the principles of the invention that the form and details of this invention may be modified without departing from these principles. We claim all modifications that fall within the spirit and scope of the claims. All publications and published patent documents cited herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually identified as being incorporated by reference.

--->

SEQUENCE LIST

<110> Monsanto Technology LLC

<120> PLANT REGULATORY ELEMENTS AND THEIR USE

<130> MONS:323WO

<140> No information

<141> 2013-12-17

<150> 61/739.720

<151> 2012-12-19

<160> 171

<210> 1

<211> 3143

<212> DNA

<213> Agrostis nebulosa

<400> 1

ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg 60

agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt 120

acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca 180

ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga 240

cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg 300

360

cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag 420

aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca 480

tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa 540

cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa 600

agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga 660

gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg 720

atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca 780

gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa 840

ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa 900

atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac 960

tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag 1020

ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata 1080

gaattctaag tatgaaatga ttttttttgtt atacaaaagg agtatatatc acaagattga 1140

tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg 1200

tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag 1260

gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt 1320

tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag 1380

ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt 1440

tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct 1500

1560

1620

taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga 1680

caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa 1740

cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc 1800

ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 1860

tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacggg 1920

gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaaatagcca 1980

gccccgtccc cagattcttt cccaacctca tctttgttcg gagcacccac acaacccgat 2040

ccccaattcc ctcgtctctc ctcgcgagcc tcgtcgaccc ccccttcaag gtacggcgat 2100

cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg cgacccggtc 2160

catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg taaaatagat 2220

ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc ctttaggaca 2280

tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct aggcagtggg 2340

gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa ctgggaaacc 2400

tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga tgagatcgat 2460

ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt ccgtggtatg 2520

atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca tttaattgtc 2580

aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg ttgggctgta 2640

gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc tgtatgtgtc 2700

acatatatct tcatgattaa tatggttgga attatctctt catcttttag atatatatgg 2760

taggtatat atgttgctgt gggttttact ggtactttat tagatatatt catgcttaga 2820

2880

gataggtata tatgttgctg atggttttac tgatacttta ttagatagta cttctttgac 2940

atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt 3000

3060

3120

gtttggtgat ccttctgcag gtg 3143

<210> 2

<211> 2005

<212> DNA

<213> Agrostis nebulosa

<400> 2

ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg 60

agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt 120

acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca 180

ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga 240

cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg 300

360

cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag 420

aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca 480

tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa 540

cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa 600

agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga 660

gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg 720

atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca 780

gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa 840

ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa 900

atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac 960

tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag 1020

ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata 1080

gaattctaag tatgaaatga ttttttttgtt atacaaaagg agtatatatc acaagattga 1140

tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg 1200

tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag 1260

gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt 1320

tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag 1380

ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt 1440

tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct 1500

1560

1620

taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga 1680

caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa 1740

cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc 1800

ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 1860

tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacggg 1920

gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaaatagcca 1980

gccccgtccc cagattcttt cccaa 2005

<210> 3

<211> 85

<212> DNA

<213> Agrostis nebulosa

<400> 3

cctcatcttt gttcggagca cccacacaac ccgatcccca attccctcgt ctctcctcgc 60

gagcctcgtc gaccccccct tcaag 85

<210> 4

<211> 1053

<212> DNA

<213> Agrostis nebulosa

<400> 4

gtacggcgat cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg 60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600

tgtatgtgtc acatatatct tcatgattaa tatggttgga attatctctt catcttttag 660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780

aacaaataag gataggtata tatgttgctg atggttttac tgatacttta ttagatagta 840

cttctttgac atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa 900

agcttgcttt ttaattattt tgatatactt ggatgatgtc atgcagcagc tatgtgtgaa 960

ttttcggccc tgtcttcata tgatgtttat ttgcttggga ctgtttcttt ggctgataac 1020

tcaccctgtt gtttggtgat ccttctgcag gtg 1053

<210> 5

<211> 2137

<212> DNA

<213> Agrostis nebulosa

<400> 5

gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct 60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aggagtata 120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420

gcccatataa gcgttcatga attgtacata tccttagatt ttttttttttg ggtaaagctc 480

gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat 540

ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg 600

tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg 660

tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag 720

cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct 780

ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt 840

ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc 900

accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc 960

gctataaata gccagccccg tccccagatt ctttcccaac ctcatctttg ttcggagcac 1020

ccacacaacc cgatccccaa ttccctcgtc tctcctcgcg agcctcgtcg accccccctt 1080

caaggtacgg cgatcgtcct ccctccctct ctctctctac cttctcttct ctagactaga 1140

tcggcgaccc ggtccatggt tagggcctgc tagttctgtt cctgtttttt ccatggctgc 1200

gaggtaaaat agatctgatg gcgttatgat ggttaactcg tcatactctt gcgatctatg 1260

gtccctttag gacatcgatt taatttcgga tggttcgaga tcggtgatcc atggttagta 1320

1380

gtaactggga aacctgggat ggttctagct gggaatcctg ggatggttct agctggttcg 1440

cagatgagat cgatttcatg gtctgctata tcttgtttcg ttgcctaggt tccgtttaat 1500

ctgtccgtgg tatgatgtta gcctttgata aggttcgatc gtgctagcta cgtcctgcgc 1560

1620

ctggttgggc tgtagatagt ttcaatctac ctgtcggttt attttattaa atttggattg 1680

gatctgtatg tgtcacatat atcttcatga ttaatatggt tggaattatc tcttcatctt 1740

ttagatatat atggataggt atatatgttg ctgtgggttt tactggtact ttattagata 1800

1860

aataaacaaa taaggatagg tatatatgtt gctgatggtt ttactgatac tttattagat 1920

agtacttctt tgacatgaag gaacatcctg cgacagctta ataattattc ttcatctaat 1980

aaaaagcttg ctttttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 2040

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 2100

taactcaccc tgttgtttgg tgatccttct gcaggtg 2137

<210> 6

<211> 999

<212> DNA

<213> Agrostis nebulosa

<400> 6

gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct 60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aggagtata 120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420

gcccatataa gcgttcatga attgtacata tccttagatt ttttttttttg ggtaaagctc 480

gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat 540

ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg 600

tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg 660

tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag 720

cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct 780

ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt 840

ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc 900

accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc 960

gctataaata gccagccccg tccccagatt ctttcccaa 999

<210> 7

<211> 1900

<212> DNA

<213> Agrostis nebulosa

<400> 7

gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata 60

120

ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta 180

agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag 240

ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt 300

360

gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag 420

ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc 480

aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga 540

tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca 600

agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac 660

ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc 720

gccgctataa atagccagcc ccgtccccag attctttccc aacctcatct ttgttcggag 780

cacccacaca acccgatccc caattccctc gtctctcctc gcgagcctcg tcgacccccc 840

cttcaaggta cggcgatcgt cctccctccc tctctctctc taccttctct tctctagact 900

agatcggcga cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc 960

tgcgaggtaa aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct 1020

atggtccctt taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta 1080

gtaccctagg cagtggggtt agatccgtgc tgttagggtt cgtagatgga ttctgattgc 1140

tcagtaactg ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt 1200

tcgcagatga gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt 1260

aatctgtccg tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg

1380

1440

ttggatctgt atgtgtcaca tatatcttca tgattaatat ggttggaatt atctcttcat 1500

cttttagata tatatggata ggtatatatg ttgctgtggg ttttactggt actttattag 1560

atatattcat gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta 1620

tctaataaac aaataaggat aggtatatat gttgctgatg gttttactga tactttatta 1680

gatagtactt ctttgacatg aaggaacatc ctgcgacagc ttaataatta ttcttcatct 1740

aataaaaagc ttgcttttta attattttga tatacttgga tgatgtcatg cagcagctat 1800

1860

tgataactca ccctgttgtt tggtgatcct tctgcaggtg 1900

<210> 8

<211> 762

<212> DNA

<213> Agrostis nebulosa

<400> 8

gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata 60

120

ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta 180

agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag 240

ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt 300

360

gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag 420

ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc 480

aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga 540

tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca 600

agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac 660

ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc 720

gccgctataa atagccagcc ccgtccccag attctttccc aa 762

<210> 9

<211> 5068

<212> DNA

<213> Arundo donax

<400> 9

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga 60

ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag 120

gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga 180

tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc 240

agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa 300

aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg 360

tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg 420

aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc 480

ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga 540

tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata 600

tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt 660

gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata 720

attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt 780

gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg 840

tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata 900

catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc 960

accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact 1020

atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc 1080

acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt 1140

gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga 1200

agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt 1260

ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg 1320

gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc 1380

ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct 1440

caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc 1500

ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc 1560

cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt 1620

caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat 1680

ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta 1740

tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa 1800

gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga 1860

gctgacaaga acctcccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg 1920

agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat 1980

catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc 2040

tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg 2100

aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt 2160

gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacggg 2220

ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta 2280

actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca 2340

ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa 2400

ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt 2460

ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac 2520

aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca 2580

gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg 2640

accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga 2700

atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc 2760

actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg 2820

agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac 2880

ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac 2940

attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa 3000

aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa 3060

accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt 3120

atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca 3180

tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa 3240

3300

ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg 3360

3420

gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca 3480

gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg 3540

gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt 3600

3660

agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaactttgaggacgaag 3720

ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc 3780

cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc 3840

aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc 3900

tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc 3960

gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa 4020

ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa 4080

ataggcaccc cgtcctcgcc tcctctcccc acctcatctc ctcctttccc gtgaaccgtg 4140

aacacaaccc gacccagatc ccctcttgcg agcttcgtcg atccctcctc cgcgtcaagg 4200

tacggagctt ctcctccccc ttcttctcta gatcggcgtg ttatgttgtt tccgtggttg 4260

cttggttgga tgaatcgaat gattcttagg gcctaggagg ctggttagat ctgttgcgtt 4320

ctgtttcgta gatggatttt ggtgtaagat caggtcggtt ccgctgttta acttgtgatg 4380

ctagtgtgat ttttgggagg atttgagttg ttaatctgggg agttgttggg aggttctcgt 4440

aggcggattg tagatgaagt cgccgcacg atttgcgtgg cttgttggggt agctagggtt 4500

agatctgctc ggatttttca ttgttactta ttgagagata atgtagctaa cctttacttg 4560

ttcatctatg tatctcgtat tcgtattcat ctggttcgat ggtgctagat agatgcgcct 4620

gatttgtccg atcgaattgg gtagcatccg cggcttgttt ggtagtgttc tgattgattt 4680

gtcgctctag atctgagtgg aataatatta catctcaaca tgttactaga aacttggttt 4740

atagctccgg atttacatgt ttattcttat gtaaggtttt aaatgaaaga tttatgctac 4800

tgctgctcgt tgatccttta gcatccacct gaggaacatg catgcatctg ttacttcttt 4860

tgatatatgc ttagatagtt gttagtatat actgctgttg ttcgatgatc cttcaggatg 4920

aacatgcatg atcatgttac ttgtttttat atgcttctgc tgttcgttga ttctttagta 4980

ctacctacct gatcatcttg catgtttcct gcttgttaga gattaattga ttaggcttac 5040

cttgttgcct ggtgattctt ccttgcag 5068

<210> 10

<211> 4114

<212> DNA

<213> Arundo donax

<400> 10

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga 60

ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag 120

gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga 180

tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc 240

agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa 300

aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg 360

tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg 420

aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc 480

ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga 540

tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata 600

tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt 660

gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata 720

attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt 780

gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg 840

tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata 900

catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc 960

accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact 1020

atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc 1080

acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt 1140

gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga 1200

agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt 1260

ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg 1320

gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc 1380

ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct 1440

caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc 1500

ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc 1560

cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt 1620

caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat 1680

ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta 1740

tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa 1800

gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga 1860

gctgacaaga acctcccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg 1920

agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat 1980

catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc 2040

tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg 2100

aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt 2160

gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacggg 2220

ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta 2280

actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca 2340

ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa 2400

ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt 2460

ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac 2520

aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca 2580

gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg 2640

accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga 2700

atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc 2760

actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg 2820

agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac 2880

ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac 2940

attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa 3000

aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa 3060

accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt 3120

atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca 3180

tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa 3240

3300

ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg 3360

3420

gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca 3480

gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg 3540

gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt 3600

3660

agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaactttgaggacgaag 3720

ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc 3780

cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc 3840

aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc 3900

tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc 3960

gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa 4020

ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa 4080

ataggcaccc cgtcctcgcc tcctctcccc acct 4114

<210> 11

<211> 85

<212> DNA

<213> Arundo donax

<400> 11

catctcctcc tttcccgtga accgtgaaca caacccgacc cagatcccct cttgcgagct 60

tcgtcgatcc ctcctccgcg tcaag 85

<210> 12

<211> 869

<212> DNA

<213> Arundo donax

<400> 12

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840

ccttgttgcc tggtgattct tccttgcag 869

<210> 13

<211> 2969

<212> DNA

<213> Arundo donax

<400> 13

gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga 60

cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct 120

agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac 180

tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc 240

ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg 300

actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt 360

ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa 420

caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg 480

gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac 540

cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat 600

660

tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag 720

agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt 780

gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat 840

tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa 900

tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac 960

cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat 1020

ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata 1080

gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact 1140

ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt 1200

ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg 1260

acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga 1320

gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga 1380

gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga 1440

gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg 1500

ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag 1560

tggtcggttt gatagttcag ggatgaaatg tgtctttgggg caaactttga ggacgaagtt 1620

gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca 1680

ctccgagccg gcatgtgggg tcccctag tcagccactg tatggcgccg actagctcaa 1740

1800

tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt 1860

gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc 1920

gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat 1980

aggcaccccg tcctcgcctc ctctccccac ctcatctcct cctttcccgt gaaccgtgaa 2040

cacaacccga cccagatccc ctcttgcgag cttcgtcgat ccctcctccg cgtcaaggta 2100

cggagcttct cctccccctt cttctctaga tcggcgtgtt atgttgtttc cgtggttgct 2160

tggttggatg aatcgaatga ttcttagggc ctaggaggct ggttagatct gttgcgttct 2220

2280

agtgtgattt ttgggaggat ttgagttgtt aatctgggag ttgttgggag gttctcgtag 2340

gcggattgta gatgaagtcg cccgcacgat ttgcgtggct tgttgggtag ctagggttag 2400

atctgctcgg atttttcatt gttacttatt gagagataat gtagctaacc tttacttgtt 2460

catctatgta tctcgtattc gtattcatct ggttcgatgg tgctagatag atgcgcctga 2520

tttgtccgat cgaattgggt agcatccgcg gcttgtttgg tagtgttctg attgatttgt 2580

2640

agctccggat ttacatgttt attcttatgt aaggttttaa atgaaagatt tatgctactg 2700

ctgctcgttg atcctttagc atccacctga ggaacatgca tgcatctgtt acttcttttg 2760

atatatgctt agatagttgt tagtatatac tgctgttgtt cgatgatcct tcaggatgaa 2820

catgcatgat catgttactt gttttatat gcttctgctg ttcgttgatt ctttagtact 2880

acctacctga tcatcttgca tgtttcctgc ttgttagaga ttaattgatt aggcttacct 2940

tgttgcctgg tgattcttcc ttgcaggtg 2969

<210> 14

<211> 2012

<212> DNA

<213> Arundo donax

<400> 14

gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga 60

cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct 120

agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac 180

tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc 240

ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg 300

actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt 360

ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa 420

caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg 480

gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac 540

cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat 600

660

tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag 720

agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt 780

gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat 840

tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa 900

tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac 960

cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat 1020

ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata 1080

gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact 1140

ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt 1200

ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg 1260

acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga 1320

gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga 1380

gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga 1440

gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg 1500

ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag 1560

tggtcggttt gatagttcag ggatgaaatg tgtctttgggg caaactttga ggacgaagtt 1620

gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca 1680

ctccgagccg gcatgtgggg tcccctag tcagccactg tatggcgccg actagctcaa 1740

1800

tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt 1860

gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc 1920

gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat 1980

aggcaccccg tcctcgcctc ctctccccac ct 2012

<210> 15

<211> 872

<212> DNA

<213> Arundo donax

<400> 15

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840

ccttgttgcc tggtgattct tccttgcagg tg 872

<210> 16

<211> 1954

<212> DNA

<213> Arundo donax

<400> 16

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260

1320

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440

tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920

gcttaccttg ttgcctggtg attcttcctt gcag 1954

<210> 17

<211> 1000

<212> DNA

<213> Arundo donax

<400> 17

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct 1000

<210> 18

<211> 1957

<212> DNA

<213> Arundo donax

<400> 18

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260

1320

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440

tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920

gcttaccttg ttgcctggtg attcttcctt gcaggtg 1957

<210> 19

<211> 1957

<212> DNA

<213> Arundo donax

<400> 19

tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60

agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120

ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180

agatattttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240

gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300

aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360

gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420

gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480

tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540

gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600

acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660

catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720

tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780

ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840

ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900

acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960

ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020

accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080

tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140

tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200

tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260

1320

1380

agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440

tacttgttca tctatgtata tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500

gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560

tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620

tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680

tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740

ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800

1860

ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920

gcttaccttg ttgcctggtg attcttcctt gcaggtg 1957

<210> 20

<211> 872

<212> DNA

<213> Arundo donax

<400> 20

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180

240

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360

gttcatctat gtatatcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540

600

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840

ccttgttgcc tggtgattct tccttgcagg tg 872

<210> 21

<211> 1712

<212> DNA

<213> Arundo donax

<400> 21

gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt 60

ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac 120

agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac 180

ggagggaggg gcactatgaa ttttttggtga cggagggagg ggcactatga atttttggct 240

ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga 300

aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa 360

gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct 420

ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct 480

caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat 540

ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct 600

cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga 660

accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata 720

aataggcacc ccgtcctcgc ctcctctccc cacctcatct cctcctttcc cgtgaaccgt 780

gaacacaacc cgacccagat cccctcttgc gagcttcgtc gatccctcct ccgcgtcaag 840

gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 900

gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 960

tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 1020

1080

taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 1140

tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 1200

gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 1260

tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 1320

tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 1380

1440

ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 1500

ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 1560

gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 1620

actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 1680

ccttgttgcc tggtgattct tccttgcagg tg 1712

<210> 22

<211> 755

<212> DNA

<213> Arundo donax

<400> 22

gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt 60

ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac 120

agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac 180

ggagggaggg gcactatgaa ttttttggtga cggagggagg ggcactatga atttttggct 240

ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga 300

aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa 360

gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct 420

ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct 480

caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat 540

ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct 600

cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga 660

accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata 720

aataggcacc ccgtcctcgc ctcctctccc cacct 755

<210> 23

<211> 3276

<212> DNA

<213> Arundo donax

<400> 23

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc 60

ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt 120

gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta 180

tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa 240

tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca 300

cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac 360

acattggtca ttcgggagg agtaatcccc catagctagt tgttttgagt ttgactaccc 420

aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag 480

gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa 540

atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc 600

tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga 660

atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct 720

tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg 780

gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag 840

aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt 900

agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg 960

aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt 1020

ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga 1080

ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg 1140

atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg 1200

aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat 1260

tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt 1320

ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga 1380

acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa 1440

tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct 1500

tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tattagaat 1560

acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc 1620

caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc 1680

agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc 1740

aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc 1800

tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc 1860

tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg 1920

agaggggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg 1980

gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgtctcatct 2040

cctcctgttc cgtgaaccgt gaacgcaacc cgacccccag atctctctcg cgagcatcgt 2100

cgatccctcc tccgcgtcaa ggtacggatc ttctccttcc tcccccttcc cctctgggtc 2160

ggcgtgtcgt gttgtttctc tagttgcttg gctggatgga tcgagtggtt cttagggctt 2220

agatggctgg ttagatctgt tgcgttctgt ttcgtagatg gatttttggt gtagatctgg 2280

taggttatgc tggttaactg gtgatgctcc tgcgattttt gggggatctg agttgttaat 2340

ctggtagttg tatggggttc tcgtagccgg attgtagatg aaatcgtccg cgcggtttgc 2400

gtggctcgtt ggttagctag ggttagatct gctcggattt ttcattgttc ctgattcaga 2460

gatgtagtta acctttactt gttcatcttt gtatctcgta ttcgtacctg catgtatgat 2520

ctgtttcgat ggtgctagat aggtgcgcct gatttgtccg atcgaatctg gtagcatgcg 2580

ctgtttgttt ggtagtgttc tgattgattt gtcgctctag atctgagtag aataggatta 2640

tttctcaaca tgatattaga agcttggttt atagctccgg attagcatgt atgttacatg 2700

tttattctta tgtaaggttt taaacggaag atatatgcta ctgctgctca ttgattcttt 2760

atcatccacc tgagtccatg catgcttctg ttacttcttt tgatatgtgc ttagatagct 2820

gttgatatgt actgctgctg ttagatgatc cttcaggatg aacatgcatg attctgttac 2880

ttgttttggt atgcttagat aaatcaagat acgcttctgc tgttcgttga ttctttagta 2940

ctacctacct gatcagctta gatagatcaa gatatgcttc tgctgttcgt tgattcttta 3000

gtaataccta cctgatcagc ttagatagat caagatacgc ttctgctgtt cgttgattct 3060

ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaaggtt gattgcttag 3120

gctcatcttt ttcttttcgt tgattctcta gtactaccta cctgataaac atgcatgttt 3180

tctgcttgtt aaagattgat tgcttagtct catctttttc tttctctttt gtctaccgcc 3240

aggcctaacc ttgttgctgg tgactctttc ttgcag 3276

<210> 24

<211> 2033

<212> DNA

<213> Arundo donax

<400> 24

ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc 60

ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt 120

gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta 180

tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa 240

tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca 300

cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac 360

acattggtca ttcgggagg agtaatcccc catagctagt tgttttgagt ttgactaccc 420

aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag 480

gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa 540

atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc 600

tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga 660

atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct 720

tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg 780

gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag 840

aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt 900

agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg 960

aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt 1020

ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga 1080

ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg 1140

atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg 1200

aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat 1260

tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt 1320

ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga 1380

acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa 1440

tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct 1500

tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tattagaat 1560

acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc 1620

caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc 1680

agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc 1740

aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc 1800

tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc 1860

tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg 1920

agaggggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg 1980

gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgt 2033

<210> 25

<211> 88

<212> DNA

<213> Arundo donax

<400> 25

ctcatctcct cctgttccgt gaaccgtgaa cgcaacccga cccccagatc tctctcgcga 60

gcatcgtcga tccctcctcc gcgtcaag 88

<210> 26

<211> 1155

<212> DNA

<213> Arundo donax

<400> 26

gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct 60

agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt 120

gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg 180

tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct 240

cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg 300

gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg 360

ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata 420

ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct 480

gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa 540

gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt 600

aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc 660

atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt 720

tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata 780

aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag 840

atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct 900

tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa 960

1020

gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt 1080

gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt 1140

gactctttct tgcag 1155

<210> 27

<211> 3250

<212> DNA

<213> Arundo donax

<400> 27

gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa 60

taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca 120

aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata 180

ctacttctta aagggctatt ctttttctga atgtcgcagt tggtttatttt aaccatatta 240

caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa 300

gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc 360

ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg 420

gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca 480

aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa 540

aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac 600

aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag 660

cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca 720

atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca 780

atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa 840

ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat 900

tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa 960

ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag 1020

1080

catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag 1140

tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca 1200

aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac 1260

1320

gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt 1380

1440

ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat 1500

atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt 1560

ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga 1620

1680

1740

caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa 1800

gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc 1860

tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa 1920

ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc 1980

ccctcctcgc gtcctctccc ccgtctcatc tcctcctgtt ccgtgaaccg tgaacgcaac 2040

ccgaccccca gatctctctc gcgagcatcg tcgatccctc ctccgcgtca aggtacggat 2100

cttctccttc ctcccccttc ccctctgggt cggcgtgtcg tgttgtttct ctagttgctt 2160

ggctggatgg atcgagtggt tcttagggct tagatggctg gttagatctg ttgcgttctg 2220

tttcgtagat ggatttttgg tgtagatctg gtaggttatg ctggttaact ggtgatgctc 2280

ctgcgatttt tgggggatct gagttgttaa tctggtagtt gtatggggtt ctcgtagccg 2340

gattgtagat gaaatcgtcc gcgcggtttg cgtggctcgt tggttagcta gggttagatc 2400

tgctcggatt tttcattgtt cctgattcag agatgtagtt aacctttact tgttcatctt 2460

tgtatctcgt attcgtacct gcatgtatga tctgtttcga tggtgctaga taggtgcgcc 2520

tgatttgtcc gatcgaatct ggtagcatgc gctgtttgtt tggtagtgtt ctgattgatt 2580

tgtcgctcta gatctgagta gaataggatt atttctcaac atgatattag aagcttggtt 2640

tatagctccg gattagcatg tatgttacat gtttattctt atgtaaggtt ttaaacggaa 2700

gatatatgct actgctgctc attgattctt tatcatccac ctgagtccat gcatgcttct 2760

gttacttctt ttgatatgtg cttagatagc tgttgatatg tactgctgct gttagatgat 2820

tatgcttaga taaatcaaga 2880

tacgcttctg ctgttcgttg attctttagt actacctacc tgatcagctt agatagatca 2940

agatatgctt ctgctgttcg ttgattcttt agtaatacct acctgatcag cttagataga 3000

tcaagatacg cttctgctgt tcgttgattc tctagtacta cctacctgat aaacatgcat 3060

3120

agtactacct acctgataaa catgcatgtt ttctgcttgt taaagattga ttgcttagtc 3180

tcatcttttt ctttctcttt tgtctaccgc caggcctaac cttgttgctg gtgactcttt 3240

cttgcaggtg 3250

<210> 28

<211> 2004

<212> DNA

<213> Arundo donax

<400> 28

gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa 60

taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca 120

aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata 180

ctacttctta aagggctatt ctttttctga atgtcgcagt tggtttatttt aaccatatta 240

caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa 300

gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc 360

ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg 420

gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca 480

aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa 540

aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac 600

aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag 660

cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca 720

atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca 780

atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa 840

ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat 900

tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa 960

ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag 1020

1080

catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag 1140

tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca 1200

aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac 1260

1320

gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt 1380

1440

ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat 1500

atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt 1560

ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga 1620

1680

1740

caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa 1800

gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc 1860

tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa 1920

ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc 1980

ccctcctcgc gtcctctccc ccgt 2004

<210> 29

<211> 1158

<212> DNA

<213> Arundo donax

<400> 29

gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct 60

agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt 120

gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg 180

tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct 240

cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg 300

gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg 360

ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata 420

ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct 480

gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa 540

gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt 600

aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc 660

atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt 720

tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata 780

aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag 840

atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct 900

tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa 960

1020

gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt 1080

gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt 1140

gactctttct tgcaggtg 1158

<210> 30

<211> 2247

<212> DNA

<213> Arundo donax

<400> 30

tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg 60

gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga 120

gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat 180

aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg 240

aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac 300

ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa 360

tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct 420

taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca 480

tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta 540

cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct 600

aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg 660

gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa 720

ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc 780

gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg 840

gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagggg 900

ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt 960

ctccataaat aggcgccccc tcctcgcgtc ctctcccccg tctcatctcc tcctgttccg 1020

tgaaccgtga acgcaacccg accccgat ctctctcgcg agcatcgtcg atccctcctc 1080

1140

tgtttctcta gttgcttggc tggatggatc gagtggttct tagggcttag atggctggtt 1200

1260

gttaactggt gatgctcctg cgatttttgg gggatctgag ttgttaatct ggtagttgta 1320

tggggttctc gtagccggat tgtagatgaa atcgtccgcg cggtttgcgt ggctcgttgg 1380

ttagctaggg ttagatctgc tcggattttt cattgttcct gattcagaga tgtagttaac 1440

ctttacttgt tcatctttgt atctcgtatt cgtacctgca tgtatgatct gtttcgatgg 1500

tgctagatag gtgcgcctga tttgtccgat cgaatctggt agcatgcgct gtttgtttgg 1560

tagtgttctg attgatttgt cgctctagat ctgagtagaa taggattatt tctcaacatg 1620

1680

taaggtttta aacggaagat atatgctact gctgctcatt gattctttat catccacctg 1740

agtccatgca tgcttctgtt acttcttttg atatgtgctt agatagctgt tgatatgtac 1800

tgctgctgtt agatgatcct tcaggatgaa catgcatgat tctgttactt gttttggtat 1860

gcttagataa atcaagatac gcttctgctg ttcgttgatt ctttagtact acctacctga 1920

tcagcttaga tagatcaaga tatgcttctg ctgttcgttg attctttagt aatacctacc 1980

tgatcagctt agatagatca agatacgctt ctgctgttcg ttgattctct agtactacct 2040

acctgataaa catgcatgtt ttctgcttgt taaaggttga ttgcttaggc tcatcttttt 2100

cttttcgttg attctctagt actacctacc tgataaacat gcatgttttc tgcttgttaa 2160

agattgattg cttagtctca tctttttctt tctcttttgt ctaccgccag gcctaacctt 2220

gttgctggtg actctttctt gcaggtg 2247

<210> 31

<211> 1001

<212> DNA

<213> Arundo donax

<400> 31

tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg 60

gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga 120

gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat 180

aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg 240

aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac 300

ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa 360

tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct 420

taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca 480

tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta 540

cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct 600

aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg 660

gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa 720

ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc 780

gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg 840

gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagggg 900

ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt 960

ctccataaat aggcgcccc tcctcgcgtc ctctcccccg t 1001

<210> 32

<211> 1942

<212> DNA

<213> Arundo donax

<400> 32

catgttgact aagagcaagt ggagcact ctcatttcat agaacaagtg atgaatgcca 60

acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata 120

atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt 180

aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa 240

atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc 300

ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag 360

taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc 420

caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc 480

ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg 540

ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac 600

ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca 660

taaataggcg ccccctcctc gcgtcctctc ccccgtctca tctcctcctg ttccgtgaac 720

cgtgaacgca acccgacccc cagatctctc tcgcgagcat cgtcgatccc tcctccgcgt 780

caaggtacgg atcttctcct tcctccccct tcccctctgg gtcggcgtgt cgtgttgttt 840

ctctagttgc ttggctggat ggatcgagtg gttcttaggg cttagatggc tggttagatc 900

tgttgcgttc tgtttcgtag atggattttt ggtgtagatc tggtaggtta tgctggttaa 960

ctggtgatgc tcctgcgatt tttgggggat ctgagttgtt aatctggtag ttgtatgggg 1020

ttctcgtagc cggattgtag atgaaatcgt ccgcgcggtt tgcgtggctc gttggttagc 1080

tagggttaga tctgctcgga tttttcattg ttcctgattc agagatgtag ttaaccttta 1140

cttgttcatc tttgtatctc gtattcgtac ctgcatgtat gatctgtttc gatggtgcta 1200

1260

ttctgattga tttgtcgctc tagatctgag tagaatagga ttatttctca acatgatatt 1320

agaagcttgg tttatagctc cggattagca tgtatgttac atgtttattc ttatgtaagg 1380

ttttaaacgg aagatatatg ctactgctgc tcattgattc tttatcatcc acctgagtcc 1440

atgcatgctt ctgttacttc ttttgatatg tgcttagata gctgttgata tgtactgctg 1500

ctgttagatg atccttcagg atgaacatgc atgattctgt tacttgtttt ggtatgctta 1560

gataaatcaa gatacgcttc tgctgttcgt tgattcttta gtactaccta cctgatcagc 1620

ttagatagat caagatatgc ttctgctgtt cgttgattct ttagtaatac ctacctgatc 1680

agcttagata gatcaagata cgcttctgct gttcgttgat tctctagtac tacctacctg 1740

ataaacatgc atgttttctg cttgttaaag gttgattgct taggctcatc tttttctttt 1800

cgttgattct ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaagatt 1860

gattgcttag tctcatcttt ttctttctct tttgtctacc gccaggccta accttgttgc 1920

tggtgactct ttcttgcagg tg 1942

<210> 33

<211> 696

<212> DNA

<213> Arundo donax

<400> 33

catgttgact aagagcaagt ggagcact ctcatttcat agaacaagtg atgaatgcca 60

acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata 120

atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt 180

aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa 240

atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc 300

ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag 360

taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc 420

caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc 480

ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg 540

ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac 600

ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca 660

taaataggcg ccccctcctc gcgtcctctc ccccgt 696

<210> 34

<211> 3511

<212> DNA

<213> Bouteloua gracilis

<400> 34

gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg 60

attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag 120

ccctagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg 180

caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct 240

gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt 300

gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg 360

cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg 420

gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca 480

cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta 540

tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag 600

660

gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca 720

gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt 780

ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta 840

tatgagtgaa ttgataaatt tattctagggt gacatggcct taacgatgaa cagtacgtgg 900

ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga 960

ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt 1020

ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca 1080

ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta 1140

taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa 1200

tgctcaagat tcattcaaca attgaaacgt

taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat 1320

1380

ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa 1440

1500

1560

1620

agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc 1680

tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac 1740

aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat 1800

ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc 1860

agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac 1920

cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta 1980

ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca 2040

ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga 2100

cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca 2160

cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg 2220

ttccgttccg ccctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag 2280

cacggggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa 2340

tagccagccc cgtccccaga ttctttccca acctcatctt tgttcggagc acgcacacaa 2400

cccgatcccc aattccctcg tctctcctcg cgagcctcgt cgaccccccc cttcaaggta 2460

cggcgatcat cctccctccc tccctctctc taccttctct tctctagact agatcggcga 2520

cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc tgcgaggtaa 2580

aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct atggtccctt 2640

taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta gtaccctagg 2700

2760

ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt tcgcagatga 2820

gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt aatctgtccg 2880

tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg cgcagcattt 2940

aattgtcagg tcataatttt tagcattcct gtttttgttt ggtttggttt tgtctggttg 3000

3060

atgtgtcaca tatatcttca tgattaagat ggttggaatt atctcttcat cttttagata 3120

3180

gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta tctaataaac 3240

3300

3360

3420

attttcggcc ctgtcttcat atgatgttta tttgcttggg actgtttctt tggctgataa 3480

cttaccctgt tgtttggtga tccttctgca g 3511

<210> 35

<211> 2371

<212> DNA

<213> Bouteloua gracilis

<400> 35

gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg 60

attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag 120

ccctagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg 180

caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct 240

gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt 300

gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg 360

cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg 420

gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca 480

cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta 540

tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag 600

660

gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca 720

gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt 780

ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta 840

tatgagtgaa ttgataaatt tattctagggt gacatggcct taacgatgaa cagtacgtgg 900

ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga 960

ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt 1020

ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca 1080

ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta 1140

taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa 1200

tgctcaagat tcattcaaca attgaaacgt

taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat 1320

1380

ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa 1440

1500

1560

1620

agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc 1680

tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac 1740

aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat 1800

ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc 1860

agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac 1920

cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta 1980

ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca 2040

ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga 2100

cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca 2160

cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg 2220

ttccgttccg ccctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag 2280

cacggggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa 2340

tagccagccc cgtccccaga ttctttccca a 2371

<210> 36

<211> 86

<212> DNA

<213> Bouteloua gracilis

<400> 36

cctcatcttt gttcggagca cgcacacaac ccgatcccca attccctcgt ctctcctcgc 60

gagcctcgtc gacccccccc ttcaag 86

<210> 37

<211> 1054

<212> DNA

<213> Bouteloua gracilis

<400> 37

gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg 60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600

tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag 660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780

aacaaataag gataggtata tgttgctgat ggttttactg atacttttatt agatagtact 840

ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt 900

gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 960

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 1020

taacttaccc tgttgtttgg tgatccttct gcag 1054

<210> 38

<211> 3142

<212> DNA

<213> Bouteloua gracilis

<400> 38

gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc 60

ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg 120

tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac 180

ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata 240

atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag 300

aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag 360

gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag 420

gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt 480

gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag 540

aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg 600

caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca 660

aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc 720

gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac 780

ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc 840

attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct 900

tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt 960

ttaaaacaat tttataagag aagctccggga gataaaaggg catctaatct atgttagaag 1020

agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt 1080

tgcttagggc acgtacacga 1140

cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca 1200

cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc 1260

ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat 1320

1380

attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt 1440

ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc 1500

atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt 1560

gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca 1620

agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa 1680

ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc 1740

ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca 1800

cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc 1860

tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt 1920

ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg 1980

tccccagatt ctttcccaac ctcatctttg ttcggagcac gcacacaacc cgatccccaa 2040

ttccctcgtc tctcctcgcg agcctcgtcg acccccccct tcaaggtacg gcgatcatcc 2100

tccctccctc cctctctcta ccttctcttc tctagactag atcggcgacc cggtccatgg 2160

ttagggcctg ctagttctgt tcctgttttt tccatggctg cgaggtaaaa tagatctgat 2220

ggcgttatga tggttaactc gtcatactct tgcgatctat ggtcccttta ggacatcgat 2280

ttaatttcgg atggttcgag atcggtgatc catggttagt accctaggca gtggggttag 2340

atccgtgctg ttagggttcg tagatggatt ctgattgctc agtaactggg aaacctggga 2400

tggttctagc tgggaatcct gggatggttc tagctggttc gcagatgaga tcgatttcat 2460

ggtctgctat atcttgtttc gttgcctagg ttccgtttaa tctgtccgtg gtatgatgtt 2520

agcctttgat aaggttcgat cgtgctagct acgtcctgcg cagcatttaa ttgtcaggtc 2580

ataattttta gcattcctgt ttttgtttgg tttggttttg tctggttgggg ctgtagatag 2640

tttcaatcta cctgtcggtt tattttatta aatttggatt ggatctgtat gtgtcacata 2700

2760

tatatatgtt gctgtgggtt ttactggtac tttattagat atattcatgc ttagatacat 2820

2880

gtatatgttg ctgatggttt tactgatact ttattagata gtactttgac atgaaggaac 2940

atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt ttaattattt 3000

3060

gtcttcatat gatgtttatt tgcttgggac tgtttctttg gctgataact taccctgttg 3120

tttggtgatc cttctgcagg tg 3142

<210> 39

<211> 1999

<212> DNA

<213> Bouteloua gracilis

<400> 39

gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc 60

ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg 120

tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac 180

ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata 240

atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag 300

aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag 360

gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag 420

gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt 480

gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag 540

aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg 600

caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca 660

aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc 720

gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac 780

ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc 840

attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct 900

tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt 960

ttaaaacaat tttataagag aagctccggga gataaaaggg catctaatct atgttagaag 1020

agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt 1080

tgcttagggc acgtacacga 1140

cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca 1200

cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc 1260

ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat 1320

1380

attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt 1440

ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc 1500

atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt 1560

gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca 1620

agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa 1680

ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc 1740

ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca 1800

cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc 1860

tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt 1920

ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg 1980

tccccagatt ctttcccaa 1999

<210> 40

<211> 1057

<212> DNA

<213> Bouteloua gracilis

<400> 40

gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg 60

cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120

taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180

ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240

aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300

ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360

tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420

ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480

tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540

ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600

tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag 660

atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720

catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780

aacaaataag gataggtata tgttgctgat ggttttactg atacttttatt agatagtact 840

ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt 900

gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 960

tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 1020

taacttaccc tgttgtttgg tgatccttct gcaggtg 1057

<210> 41

<211> 2165

<212> DNA

<213> Bouteloua gracilis

<400> 41

gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct 60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata 120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420

gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc 480

tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg 540

atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag 600

cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc 660

cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca 720

agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat 780

ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt 840

ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc 900

agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct 960

tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc 1020

aacctcatct ttgttcggag cacgcacaca acccgatccc caattccctc gtctctcctc 1080

gcgagcctcg tcgacccccc ccttcaaggt acggcgatca tcctccctcc ctccctctct 1140

ctaccttctc ttctctagac tagatcggcg acccggtcca tggttagggc ctgctagttc 1200

tgttcctgtt ttttccatgg ctgcgaggta aaatagatct gatggcgtta tgatggttaa 1260

ctcgtcatac tcttgcgatc tatggtccct ttaggacatc gatttaattt cggatggttc 1320

gagatcggtg atccatggtt agtaccctag gcagtggggt tagatccgtg ctgttagggt 1380

tcgtagatgg attctgattg ctcagtaact gggaaacctg ggatggttct agctgggaat 1440

1500

ttcgttgcct aggttccgtt taatctgtcc gtggtatgat gttagccttt gataaggttc 1560

gatcgtgcta gctacgtcct gcgcagcatt taattgtcag gtcataattt ttagcattcc 1620

tgtttttgtt tggtttggtt ttgtctggtt gggctgtaga tagtttcaat ctacctgtcg 1680

gtttatttta ttaaatttgg attggatctg tatgtgtcac atatatcttc atgattaaga 1740

tggttggaat tatctcttca tcttttagat atatatggat aggtatatat gttgctgtgg 1800

gttttactgg tactttatta gatatattca tgcttagata catgaagcaa cgtgctgtta 1860

cagtttaata attcttgttt atctaataaa caaataagga taggtatatg ttgctgatgg 1920

ttttactgat actttattag atagtacttt gacatgaagg aacatcctgc gacagcttaa 1980

taattattct tcatctaata aaaagcttgc

ttggatgatg tcatgcagca gctatgtgtg aattttcggc cctgtcttca tatgatgttt 2100

atttgcttgg gactgtttct ttggctgata acttaccctg ttgtttggtg atccttctgc 2160

aggtg 2165

<210> 42

<211> 1022

<212> DNA

<213> Bouteloua gracilis

<400> 42

gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct 60

ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata 120

ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180

240

gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300

360

cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420

gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc 480

tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg 540

atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag 600

cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc 660

cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca 720

agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat 780

ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt 840

ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc 900

agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct 960

tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc 1020

aa 1022

<210> 43

<211> 1903

<212> DNA

<213> Bouteloua gracilis

<400> 43

actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga 60

tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg 120

ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat 180

tgtacatatc cttagatgtt ttttttttttg ggtaaagctc gagcttctct atctaaaagt 240

agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt 300

360

ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc 420

caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg 480

agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt 540

ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 600

tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg 660

caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc 720

cgctataaat agccagcccc gtccccagat tctttcccaa cctcatcttt gttcggagca 780

cgcacacaac ccgatcccca attccctcgt ctctcctcgc gagcctcgtc gacccccccc 840

ttcaaggtac ggcgatcatc ctccctccct ccctctctct accttctctt ctctagacta 900

gatcggcgac ccggtccatg gttagggcct gctagttctg ttcctgtttt ttccatggct 960

gcgaggtaaa atagatctga tggcgttatg atggttaact cgtcatactc ttgcgatcta 1020

tggtcccttt aggacatcga tttaatttcg gatggttcga gatcggtgat ccatggttag 1080

taccctaggc agtggggtta gatccgtgct gttagggttc gtagatggat tctgattgct 1140

cagtaactgg gaaacctgggg atggttctag ctgggaatcc tgggatggtt ctagctggtt 1200

1260

atctgtccgt ggtatgatgt tagcctttga taaggttcga tcgtgctagc tacgtcctgc 1320

1380

gtctggttgg gctgtagata gtttcaatct acctgtcggt ttattttatt aaatttggat 1440

tggatctgta tgtgtcacat atatcttcat gattaagatg gttggaatta tctcttcatc 1500

ttttagatat atatggatag gtatatatgt tgctgtgggt tttactggta ctttattaga 1560

tgctgttaca gtttaataat tcttgtttat 1620

1680

agtactttga catgaaggaa catcctgcga cagcttaata attattcttc atctaataaa 1740

aagcttgctt tttaattatt ttaattattt tgatatactt ggatgatgtc atgcagcagc 1800

1860

ggctgataac ttaccctgtt gtttggtgat ccttctgcag gtg 1903

<210> 44

<211> 760

<212> DNA

<213> Bouteloua gracilis

<400> 44

actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga 60

tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg 120

ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat 180

tgtacatatc cttagatgtt ttttttttttg ggtaaagctc gagcttctct atctaaaagt 240

agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt 300

360

ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc 420

caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg 480

agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt 540

ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 600

tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg 660

caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc 720

cgctataaat agccagcccc gtccccagat tctttcccaa 760

<210> 45

<211> 3234

<212> DNA

<213> Bouteloua gracilis

<400> 45

ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag 60

ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag 120

tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg 180

240

gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta 300

taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt 360

tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa 420

atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc 480

catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca 540

agtttttttt ttcttgcaaa aattgtttct tttttttttaa aaaagtataa tttggatcgt 600

gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt 660

gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat 720

aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat 780

taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat 840

tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg 900

tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc 960

taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc 1020

accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga 1080

aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt 1140

taccatttttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt 1200

ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac 1260

acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag 1320

attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc 1380

ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa 1440

1500

aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa 1560

agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt 1620

tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc 1680

tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt 1740

cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt 1800

ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg 1860

agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc 1920

cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg cctcctctca 1980

cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc 2040

cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa 2100

cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct 2160

cgtcgcgagc ctcgtcgatc cccgcttcaa ggtacagcga tcgatcgatc atcctcgctc 2220

tctctacctt ctctctctta gggcgtgctg gttctgttcc tgtttttcca tggctgcgag 2280

gtacaataga ttggcgattc atggttaggg cctgctagtt ctgttcctgt tttttttttt 2340

tccatggctg cgaggcacaa tagatctgat ggcgttatga tggttaactt gtcatactct 2400

2460

ctgtgatcca tggttagtac cctaggcagt ggggttagat ccgtgctgtt atggttcgta 2520

2580

aagatcgatt tcatgatatg ctatatcttg tttggttgcc gtggttccgt taaatctgtc 2640

tgttatgatc ttagtctttg ataaggttcg gtcgtgctag ctacgtcctg tgcagcactt 2700

aattgtcagg tcataatttt tagcatgcct tttttttatt ggtttggttt tgtctgactg 2760

2820

tgtttatttt attaaatttg gatctgtatg tgtgtcatat atcttcatct tttagatata 2880

2940

3000

taaggatagg tgctgcagtt agttttactg gtactttttt tgacatgaac ctacggctta 3060

ataattagtc ttcatcaaat aaaaagcata tttttaatt atttcgatat acttgaatga 3120

tgtcatatgc agcatctgtg tgaatttttg gccctgtctt catatgatgt ttatttgctt 3180

gggactgttt ctttggctga taactcaccc tgttgtttgg tgatccttct gcag 3234

<210> 46

<211> 2100

<212> DNA

<213> Bouteloua gracilis

<400> 46

ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag 60

ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag 120

tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg 180

240

gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta 300

taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt 360

tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa 420

atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc 480

catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca 540

agtttttttt ttcttgcaaa aattgtttct tttttttttaa aaaagtataa tttggatcgt 600

gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt 660

gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat 720

aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat 780

taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat 840

tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg 900

tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc 960

taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc 1020

accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga 1080

aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt 1140

taccatttttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt 1200

ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac 1260

acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag 1320

attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc 1380

ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa 1440

1500

aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa 1560

agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt 1620

tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc 1680

tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt 1740

cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt 1800

ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg 1860

agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc 1920

cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg cctcctctca 1980

cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc 2040

cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa 2100

<210> 47

<211> 91

<212> DNA

<213> Bouteloua gracilis

<400> 47

cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct 60

cgtcgcgagc ctcgtcgatc cccgcttcaa g 91

<210> 48

<211> 1043

<212> DNA

<213> Bouteloua gracilis

<400> 48

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg 480

tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt 540

tttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact 600

gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt 660

gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt 720

actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt 780

taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg 840

tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat 900

tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg 960

ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct 1020

gttgtttggt gatccttctg cag 1043

<210> 49

<211> 3176

<212> DNA

<213> Bouteloua gracilis

<400> 49

aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc 60

aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt 120

atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacgggg 180

cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta 240

ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa 300

cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat 360

aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca 420

agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc 480

tcaagtttttt ttttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat 540

cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg 600

cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt 660

tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat 720

gattaaatca tataaagttt ctaagtcttg tttgacaaga ttttttttaga ttttcatcta 780

aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa 840

aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa 900

ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat 960

gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag 1020

agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg 1080

1140

tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg 1200

aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta 1260

gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa 1320

agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt 1380

gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca 1440

aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt 1500

gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat 1560

ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc 1620

1680

agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc 1740

cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa cggagaacc accgccagcg 1800

ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc 1860

tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc 1920

tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc 1980

ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc 2040

caacctcatc ttctcgtgtt gttcggccca acccgatcga tccccaattc cctcgtcgtc 2100

tctcgtcgcg agcctcgtcg atccccgctt caaggtacag cgatcgatcg atcatcctcg 2160

ctctctctac cttctctctc ttagggcgtg ctggttctgt tcctgttttt ccatggctgc 2220

gaggtacaat agattggcga ttcatggtta gggcctgcta gttctgttcc tgtttttttt 2280

ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac 2340

tcttgcgatc tatggtccct ttaggagttt aggacatcga tttaatttcg gatagttcga 2400

gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc 2460

gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca 2520

gtaagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct 2580

gtctgttatg atcttagtct tgataaggtt cggtcgtgct agctacgtcc tgtgcagcac 2640

ttaattgtca ggtcataatt tttagcatgc ctttttttta ttggtttggt tttgtctgac 2700

tgggctgtag atagtttcaa tctttgtctg actgggctgt agatagtttc aatcttcctg 2760

tctgtttatt ttattaaatt tggatctgta tgtgtgtcat atatcttcat cttttagata 2820

2880

2940

aataaggata ggtgctgcag ttagttttac tggtactttt tttgacatga acctacggct 3000

taataattag tcttcatcaa ataaaaagca tattttttaa ttatttcgat atacttgaat 3060

gatgtcatat gcagcatctg tgtgaatttt tggccctgtc ttcatatgat gtttatttgc 3120

ttgggactgt ttctttggct gataactcac cctgttgttt ggtgatcctt ctgcag 3176

<210> 50

<211> 2043

<212> DNA

<213> Bouteloua gracilis

<400> 50

aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc 60

aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt 120

atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacgggg 180

cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta 240

ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa 300

cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat 360

aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca 420

agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc 480

tcaagtttttt ttttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat 540

cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg 600

cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt 660

tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat 720

gattaaatca tataaagttt ctaagtcttg tttgacaaga ttttttttaga ttttcatcta 780

aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa 840

aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa 900

ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat 960

gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag 1020

agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg 1080

1140

tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg 1200

aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta 1260

gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa 1320

agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt 1380

gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca 1440

aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt 1500

gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat 1560

ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc 1620

1680

agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc 1740

cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa cggagaacc accgccagcg 1800

ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc 1860

tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc 1920

tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc 1980

ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc 2040

caa 2043

<210> 51

<211> 1042

<212> DNA

<213> Bouteloua gracilis

<400> 51

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020

ttgtttggtg atccttctgc ag 1042

<210> 52

<211> 3139

<212> DNA

<213> Bouteloua gracilis

<400> 52

gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat 60

gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt 120

atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa 180

gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc 240

aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata 300

atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg 360

aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga 420

atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt 480

aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt 540

aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa 600

aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa 660

caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat 720

ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa 780

gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc 840

ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat 900

gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc 960

ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct 1020

aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt 1080

tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc 1140

acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag 1200

1260

aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata 1320

1380

ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt 1440

ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag 1500

cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta 1560

taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc 1620

ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt 1680

caagtgacct cagtgagcc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac 1740

cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc 1800

tccctggcgt ctggtcccct ccggagactt ccgctccacc tcccaccggt ggcggtttcc 1860

gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacggggg 1920

attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc 1980

ccatccccag cttcttcccc aacctcatct tctcgtgttg ttcggcccaa cccgatcgat 2040

ccccaattcc ctcgtcgtct ctcgtcgcga gcctcgtcga tccccgcttc aaggtacagc 2100

gatcgatcga tcatcctcgc tctctctacc ttctctctct tagggcgtgc tggttctgtt 2160

cctgtttttc catggctgcg aggtacaata gattggcgat tcatggttag ggcctgctag 2220

ttctgttcct gtttttttttt tttccatggc tgcgaggcac aatagatctg atggcgttat 2280

gatggttaac ttgtcatact cttgcgatct atggtccctt taggagttta ggacatcgat 2340

ttaatttcgg atagttcgag atctgtgatc catggttagt accctaggca gtggggttag 2400

atccgtgctg ttatggttcg tagatggatt ctgattgctc agtaactggg aatcctggga 2460

2520

ccgtggttcc gttaaatctg tctgttatga tcttagtctt tgataaggtt cggtcgtgct 2580

agctacgtcc tgtgcagcac ttaattgtca ggtcataatt tttagcatgc ctttttttta 2640

ttggtttggt tttgtctgac tgggctgtag atagtttcaa tctttgtctg actgggctgt 2700

agatagtttc aatcttcctg tctgtttatt ttattaaatt tggatctgta tgtgtgtcat 2760

atatcttcat cttttagata tatcgatagg tatatatgtt gctgtcgttt tttactgttc 2820

2880

2940

tttgacatga acctacggct taataattag tcttcatcaa ataaaaagca tattttttaa 3000

ttatttcgat atacttgaat gatgtcatat gcagcatctg tgtgaatttt tggccctgtc 3060

ttcatatgat gtttatttgc ttgggactgt ttctttggct gataactcac cctgttgttt 3120

ggtgatcctt ctgcaggtg 3139

<210> 53

<211> 2002

<212> DNA

<213> Bouteloua gracilis

<400> 53

gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat 60

gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt 120

atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa 180

gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc 240

aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata 300

atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg 360

aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga 420

atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt 480

aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt 540

aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa 600

aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa 660

caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat 720

ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa 780

gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc 840

ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat 900

gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc 960

ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct 1020

aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt 1080

tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc 1140

acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag 1200

1260

aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata 1320

1380

ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt 1440

ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag 1500

cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta 1560

taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc 1620

ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt 1680

caagtgacct cagtgagcc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac 1740

cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc 1800

tccctggcgt ctggtcccct ccggagactt ccgctccacc tcccaccggt ggcggtttcc 1860

gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacggggg 1920

attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc 1980

ccatccccag cttcttcccc aa 2002

<210> 54

<211> 1046

<212> DNA

<213> Bouteloua gracilis

<400> 54

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg 480

tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt 540

tttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact 600

gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt 660

gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt 720

actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt 780

taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg 840

tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat 900

tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg 960

ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct 1020

gttgtttggt gatccttctg caggtg 1046

<210> 55

<211> 2160

<212> DNA

<213> Bouteloua gracilis

<400> 55

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540

tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020

ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt 1080

ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc 1140

gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg 1200

1260 cgaggtacaa tagattggcg attcatggtt agggcctgct

ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac 1320

1380

gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc 1440

gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca 1500

gataagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct 1560

gtctgttatg atcttagtct ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca 1620

1680

ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct 1740

1800

atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat 1860

gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac 1920

aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc 1980

ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa 2040

tgtgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg 2100

cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg 2160

<210> 56

<211> 1024

<212> DNA

<213> Bouteloua gracilis

<400> 56

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540

tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020

ccaa 1024

<210> 57

<211> 1045

<212> DNA

<213> Bouteloua gracilis

<400> 57

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120

ctgctagttc tgttcctgtt ttttttttttc catggctgcg aggcacaata gatctgatgg 180

cgttatgatg gttaacttgt catactcttg cgatctatgg tccctttagg agtttaggac 240

atcgatttaa tttcggatag ttcgagatct gtgatccatg gttagtaccc taggcagtgg 300

ggttagatcc gtgctgttat ggttcgtaga tggattctga ttgctcagta actgggaatc 360

ctgggatggt tctagctggt tcgcagataa gatcgatttc atgatatgct atatcttgtt 420

tggttgccgt ggttccgtta aatctgtctg ttatgatctt agtctttgat aaggttcggt 480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020

ttgtttggtg atccttctgc aggtg 1045

<210> 58

<211> 2160

<212> DNA

<213> Bouteloua gracilis

<400> 58

gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60

ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120

ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180

gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240

agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300

aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360

tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420

aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480

tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540

tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600

660

gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720

ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780

gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840

ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900

ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960

cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020

ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt 1080

ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc 1140

gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg 1200

1260 cgaggtacaa tagattggcg attcatggtt agggcctgct

tttttccatg gctgcgaggc acaatagatc tgatggcgtt atgatggtta acttgtcata 1320

ctcttgcgat ctatggtccc tttaggagtt taggacatcg atttaatttc ggatagttcg 1380

agatctgtga tccatggtta gtaccctagg cagtggggtt agatccgtgc tgttatggtt 1440

cgtagatgga ttctgattgc tcagtaactg ggaatcctgg gatggttcta gctggttcgc 1500

agataagatc gatttcatga tatgctatat cttgtttggt tgccgtggtt ccgttaaatc 1560

tgtctgttat gatcttagtc ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca 1620

1680

ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct 1740

1800

atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat 1860

gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac 1920

aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc 1980

ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa 2040

tgtgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg 2100

cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg 2160

<210> 59

<211> 1045

<212> DNA

<213> Bouteloua gracilis

<400> 59

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360

cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 480

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540

600

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660

tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720

ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780

aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020

ttgtttggtg atccttctgc aggtg 1045

<210> 60

<211> 1885

<212> DNA

<213> Bouteloua gracilis

<400> 60

caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag 60

taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc 120

aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gccgacaa 180

aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact 240

agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc 300

ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt 360

tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc 420

ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga 480

caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca 540

cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc 600

ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc 660

acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata 720

gccagcccca tccccagctt cttccccaac ctcatcttct cgtgttgttc ggcccaaccc 780

gatcgatccc caattccctc gtcgtctctc gtcgcgagcc tcgtcgatcc ccgcttcaag 840

gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 900

ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 960

ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 1020

gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 1080

catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 1140

gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 1200

1260

ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 1320

cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 1380

tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg 1440

ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 1500

1560

1620

1680

actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 1740

ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 1800

cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1860

ttgtttggtg atccttctgc aggtg 1885

<210> 61

<211> 749

<212> DNA

<213> Bouteloua gracilis

<400> 61

caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag 60

taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc 120

aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gccgacaa 180

aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact 240

agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc 300

ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt 360

tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc 420

ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga 480

caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca 540

cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc 600

ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc 660

acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata 720

gccagcccca tccccagctt cttccccaa 749

<210> 62

<211> 6813

<212> DNA

<213> Miscanthus sinesis

<400> 62

agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac 60

tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa 120

aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg 180

gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta 240

attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg 300

acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat 360

gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct 420

ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg 480

taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt 540

acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc 600

ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg 660

acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc 720

ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag 780

gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc 840

cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag 900

tattgataa 960

ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa 1020

ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa 1080

cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt 1140

atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat 1200

catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac 1260

gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg 1320

catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt 1380

tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc 1440

tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc 1500

gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc 1560

tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg 1620

gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc 1680

gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga 1740

agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta 1800

1860

caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg 1920

ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga 1980

cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt 2040

gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt 2100

atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca 2160

gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga 2220

tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg 2280

atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg 2340

tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg 2400

gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt 2460

acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc 2520

cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta 2580

cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc 2640

tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa 2700

aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga 2760

cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct 2820

cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga 2880

actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca 2940

tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct 3000

3060

3120

gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg 3180

ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt 3240

tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg 3300

aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta 3360

aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt 3420

3480

tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc 3540

tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc 3600

taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca 3660

acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag 3720

cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag 3780

tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat 3840

attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa 3900

3960

cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag 4020

ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac 4080

attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag 4140

gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt 4200

gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc 4260

ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa 4320

atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt 4380

gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc 4440

tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg 4500

acaagtctaa cggacaccaa cggcgaacc accagcgccg agccaagcga agcagactgc 4560

agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc 4620

tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt 4680

ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc 4740

ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc 4800

ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac 4860

ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct 4920

accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct 4980

gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat 5040

gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc 5100

taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt 5160

ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga 5220

tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt 5280

5340

gtttggtttg gtttcgtctg atttggctgt cgttctagat cagagtagaa actgtttcaa 5400

actacctgtt ggatttatta aggtagcgtt tggttcctgg tatcgaatca tacacgcacc 5460

agtgcatctt ggatagccag ctggggccca cctgtccaac cgtttggttg ccggatcgaa 5520

cgagtccatt caagaccgaa ccatgcagag caatcgaata ttctcttgtg acgctgtatc 5580

atccagttcg gcaaaaaaca ccgaatgccg ccatacagga caccgtactg agcgtctgca 5640

actctgcatc ccgctcactg ctcacatctc cgcttgccgc ctcacccatc cgactcagac 5700

cagagccaca cggattactg ctgctggtgt gtgtattaac aaaagatcca tttgaccgga 5760

gcacatgcag cttggatgga aaaaatttat tatattcgtc agtgctgcat atgtactcat 5820

acttgcatga tggttttatt tattcgacct catcagtcct ggcactatgg aaagtcattg 5880

tagtatagat tttttaatat aatataaatc attggtgact tatcttgctt aattttattt 5940

tcttattatg aaatatcgtt gcattcataa tagcaaattt gtgcaaatat atagaatcta 6000

cgtgaaattc ttggttggac caatacaaca aacccctcaa acattctctt gtactgaacc 6060

ataccattcc gtacaaccat ccaaacaaaa atcatgtatc atcatgtaca tgtaaccaaa 6120

caattaacac gcaccatcct attcagactt gtctcatcca taatctatcc atccaggatg 6180

atccatccca ttcatctata tacacccaat caaacgctac ctaaaatttg gatctgtatg 6240

6300

6360

6420

6480

atgctacagt tcaaaaattc ttgttcatct cataaacaaa aaggaggtgt atatgttgct 6540

gtgggtttta ctggtacttt attagatata tacatgctta catagatgaa gcaacatgct 6600

gctatggtgt ttaataatta ttgtttatct aataaacaaa catgcttttt aattatcttg 6660

atatgtttgg atgatggcat atgcagcagc tatgtgtgga ttttaaatac ccagcatcat 6720

6780 gagcatgcat gaccctgcct tagtatgcag

tactcatcct ttagttcggt cactcttctg cag 6813

<210> 63

<211> 5359

<212> DNA

<213> Miscanthus sinesis

<400> 63

agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac 60

tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa 120

aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg 180

gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta 240

attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg 300

acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat 360

gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct 420

ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg 480

taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt 540

acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc 600

ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg 660

acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc 720

ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag 780

gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc 840

cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag 900

tattgataa 960

ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa 1020

ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa 1080

cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt 1140

atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat 1200

catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac 1260

gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg 1320

catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt 1380

tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc 1440

tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc 1500

gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc 1560

tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg 1620

gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc 1680

gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga 1740

agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta 1800

1860

caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg 1920

ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga 1980

cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt 2040

gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt 2100

atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca 2160

gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga 2220

tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg 2280

atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg 2340

tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg 2400

gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt 2460

acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc 2520

cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta 2580

cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc 2640

tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa 2700

aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga 2760

cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct 2820

cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga 2880

actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca 2940

tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct 3000

3060

3120

gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg 3180

ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt 3240

tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg 3300

aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta 3360

aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt 3420

3480

tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc 3540

tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc 3600

taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca 3660

acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag 3720

cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag 3780

tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat 3840

attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa 3900

3960

cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag 4020

ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac 4080

attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag 4140

gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt 4200

gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc 4260

ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa 4320

atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt 4380

gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc 4440

tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg 4500

acaagtctaa cggacaccaa cggcgaacc accagcgccg agccaagcga agcagactgc 4560

agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc 4620

tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt 4680

ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc 4740

ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc 4800

ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac 4860

ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct 4920

accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct 4980

gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat 5040

gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc 5100

taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt 5160

ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga 5220

tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt 5280

5340

gtttggtttg gtttcgtct 5359

<210> 64

<211> 63

<212> DNA

<213> Miscanthus sinesis

<400> 64

gatttggctg tcgttctaga tcagagtaga aactgtttca aactacctgt tggatttatt 60

aag 63

<210> 65

<211> 1391

<212> DNA

<213> Miscanthus sinesis

<400> 65

gtagcgtttg gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct 60

ggggcccacc tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc 120

atgcagagca atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc 180

gaatgccgcc atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct 240

cacatctccg cttgccgcct cacccatccg actcagacca gagccacacg gattactgct 300

gctggtgtgt gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa 360

aaatttatta tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta 420

ttcgacctca tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa 480

tataaatcat tggtgactta tcttgcttaa tttttattttc ttattatgaa atatcgttgc 540

attcataata gcaaatttgt gcaaatatat agaatctacg tgaaattctt ggttggacca 600

atacaacaaa cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc 660

aaacaaaaat catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat 720

tcagacttgt ctcatccata atctatccat ccaggatgat ccatcccatt catctatata 780

cacccaatca aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga 840

tggatggaaa tatctcttta tcttttagat atggataggt atatatgttg ctgtgggttt 900

gttagttata tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat 960

tcttgttcat ctcaacaaat aacgataggc gtatatgttg ctgtgttttt tactggtact 1020

ttgttagata tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt 1080

gttcatctca taaacaaaaa ggaggtgtat atgttgctgt gggttttact ggtactttat 1140

tagatatata catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt 1200

gtttatctaa taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat 1260

gcagcagcta tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta 1320

gtatgcagtt atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca 1380

ctcttctgca g 1391

<210> 66

<211> 4402

<212> DNA

<213> Miscanthus sinesis

<400> 66

cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg 60

cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc 120

agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga 180

cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt 240

ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga 300

cgtccgga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg 360

ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac 420

ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga 480

ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc 540

gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg 600

ttttatttgg ttttccctttg aaatcttcta atttagcttt catagaaata atctaggtat 660

tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt 720

ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt 780

ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct 840

aactattact ccgtatatga gtgagttgtt aaattttattc caggtgaaat ggcattaacg 900

atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc 960

atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa 1020

gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt 1080

ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata 1140

aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt 1200

gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc 1260

1320

tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc 1380

gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag 1440

tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa 1500

gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa 1560

tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc 1620

caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc 1680

tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta 1740

gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct 1800

tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt 1860

ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg 1920

gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt 1980

tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc 2040

ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga 2100

caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg 2160

agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct 2220

ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg 2280

ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg 2340

ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaaatagc 2400

cagccccatc cctcgtctct cgtgttgttc ggagcgcaca cacaacccga tccccaatca 2460

atcgatcccc gcttcaaggt acggcgatcc tcctccctct ctctctacct tctcttctct 2520

acactagatc ggcggtccat ggttagggcc tgctagttcc gttcctgttt ttccatggct 2580

gcgaggtaca atagatctga tggcgttatg atggttaact tgtcatgctt ttgcgattta 2640

tagtcccttt agatagttcg agatcggtga tccatggtta gtaccctagg ctgtggagtc 2700

gggttagatc cgcgctgtta gggttcgtat atggaggcga gctgttctga ttgttaactt 2760

gctgggaatc ctgggatggt tctagctgtt ccgcagatga gatcgatttc atgatctgct 2820

gtatctatcc gtggtatgat gttagccttt gatatggttc gatcgtgcta gctacgtcct 2880

2940

cgtctgattt ggctgtcgtt ctagatcaga gtagaaactg tttcaaacta cctgttggat 3000

ttattaaggt agcgtttggt tcctggtatc gaatcataca cgcaccagtg catcttggat 3060

agccagctgg ggcccacctg tccaaccgtt tggttgccgg atcgaacgag tccattcaag 3120

accgaaccat gcagagcaat cgaatattct cttgtgacgc tgtatcatcc agttcggcaa 3180

aaaacaccga atgccgccat acaggacacc gtactgagcg tctgcaactc tgcatcccgc 3240

tcactgctca catctccgct tgccgcctca cccatccgac tcagaccaga gccacacgga 3300

ttactgctgc tggtgtgtgt attaacaaaa gatccatttg accggagcac atgcagcttg 3360

gatggaaaaa atttattata ttcgtcagtg ctgcatatgt actcatactt gcatgatggt 3420

tttatttatt cgacctcatc agtcctggca ctatggaaag tcattgtagt atagattttt 3480

taatataata taaatcattg gtgacttatc ttgcttaatt ttatttttctt attatgaaat 3540

atcgttgcat tcataatagc aaatttgtgc aaatatatag aatctacgtg aaattcttgg 3600

ttggaccaat acaacaaacc cctcaaacat tctcttgtac tgaaccatac cattccgtac 3660

aaccatccaa acaaaaatca tgtatcatca tgtacatgta accaaacaat taacacgcac 3720

catcctattc agacttgtct catccataat ctatccatcc aggatgatcc atcccattca 3780

tctatataca cccaatcaaa cgctacctaa aatttggatc tgtatgtgtc acatatatct 3840

3900

gtgggtttgt tagttata tatacgtgct tacatacgtg aagaaacctg ctgctacagt 3960

ttaataattc ttgttcatct caacaaataa cgataggcgt atatgttgct gtgtttttta 4020

4080

aaattcttgt tcatctcata aacaaaaagg aggtgtatat gttgctgtgg gttttactgg 4140

tactttatta gatatataca tgcttacata gatgaagcaa catgctgcta tggtgtttaa 4200

taattattgt ttatctaata aacaaacatg cttttttaatt atcttgatat gtttggatga 4260

tggcatatgc agcagctatg tgtggatttt aaatacccag catcatgagc atgcatgacc 4320

ctgccttagt atgcagttat ttgcttgaga ctgtttcttt tgttgatact catcctttag 4380

ttcggtcact cttctgcagg tg 4402

<210> 67

<211> 2423

<212> DNA

<213> Miscanthus sinesis

<400> 67

cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg 60

cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc 120

agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga 180

cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt 240

ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga 300

cgtccgga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg 360

ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac 420

ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga 480

ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc 540

gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg 600

ttttatttgg ttttccctttg aaatcttcta atttagcttt catagaaata atctaggtat 660

tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt 720

ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt 780

ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct 840

aactattact ccgtatatga gtgagttgtt aaattttattc caggtgaaat ggcattaacg 900

atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc 960

atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa 1020

gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt 1080

ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata 1140

aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt 1200

gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc 1260

1320

tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc 1380

gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag 1440

tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa 1500

gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa 1560

tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc 1620

caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc 1680

tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta 1740

gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct 1800

tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt 1860

ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg 1920

gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt 1980

tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc 2040

ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga 2100

caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg 2160

agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct 2220

ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg 2280

ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg 2340

ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaaatagc 2400

cagccccatc cctcgtctct cgt 2423

<210> 68

<211> 55

<212> DNA

<213> Miscanthus sinesis

<400> 68

gttgttcgga gcgcacacac aacccgatcc ccaatcaatc gatccccgct tcaag 55

<210> 69

<211> 1924

<212> DNA

<213> Miscanthus sinesis

<400> 69

gtacggcgat ccctcctccct ctctctctac cttctcttct ctacactaga tcggcggtcc 60

atggttaggg cctgctagtt ccgttcctgt ttttccatgg ctgcgaggta caatagatct 120

gatggcgtta tgatggttaa cttgtcatgc ttttgcgatt tatagtccct ttagatagtt 180

cgagatcggt gatccatggt tagtacccta ggctgtggag tcgggttaga tccgcgctgt 240

tagggttcgt atatgggaggc gagctgttct gattgttaac ttgctgggaa tcctgggatg 300

gttctagctg ttccgcagat gagatcgatt tcatgatctg ctgtatctat ccgtggtatg 360

atgttagcct ttgatatggt tcgatcgtgc tagctacgtc ctgtgcactt aattgtcagg 420

tcataatttt tactatactt ttttttttggt ttggtttggt ttcgtctgat ttggctgtcg 480

ttctagatca gagtagaaac tgtttcaaac tacctgttgg atttattaag gtagcgtttg 540

gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct ggggcccacc 600

tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc atgcagagca 660

atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc gaatgccgcc 720

atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct cacatctccg 780

cttgccgcct cacccatccg actcagacca gagccacacg gattactgct gctggtgtgt 840

gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa aaatttatta 900

tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta ttcgacctca 960

tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa tataaatcat 1020

1080

1140

cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc aaacaaaaat 1200

catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat tcagacttgt 1260

ctcatccata atctatccat ccaggatgat ccatcccatt catctatata cacccaatca 1320

aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga tggatggaaa 1380

1440

tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat tcttgttcat 1500

1560

tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt gttcatctca 1620

taaacaaaaa gggaggtgtat atgttgctgt

catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt gtttatctaa 1740

taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat gcagcagcta 1800

tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta gtatgcagtt 1860

atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca ctcttctgca 1920

ggtg 1924

<210> 70

<211> 3426

<212> DNA

<213> Miscanthus sinesis

<400> 70

gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa 60

attacattttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt 120

gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct 180

gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt 240

ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga 300

360

gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt 420

taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca 480

ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc 540

atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc 600

660

gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg 720

gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac 780

tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa 840

tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc 900

ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc 960

1020

gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg 1080

taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa cggcgaacc 1140

accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg 1200

gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac 1260

ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc 1320

1380

ccgctccgtc ccttttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt 1440

ctctcgtgtt gttcggagcg cacacacaac ccgatcccca atcaatcgat ccccgcttca 1500

aggtacggcg atcctcctcc ctctctctct accttctctt ctctacacta gatcggcggt 1560

ccatggttag ggcctgctag ttccgttcct gtttttccat ggctgcgagg tacaatagat 1620

ctgatggcgt tatgatggtt aacttgtcat gcttttgcga tttatagtcc ctttagatag 1680

ttcgagatcg gtgatccatg gttagtaccc taggctgtgg agtcgggtta gatccgcgct 1740

gttagggttc gtatatggag gcgagctgtt ctgattgtta acttgctggg aatcctggga 1800

1860

tgatgttagc ctttgatatg gttcgatcgt gctagctacg tcctgtgcac ttaattgtca 1920

ggtcataatt tttactatac ttttttttttg gtttggtttg gtttcgtctg atttggctgt 1980

cgttctagat cagagtagaa actgtttcaa actacctgtt ggatttatta aggtagcgtt 2040

tggttcctgg tatcgaatca tacacgcacc agtgcatctt ggatagccag ctggggccca 2100

2160

caatcgaata ttctcttgtg acgctgtatc atccagttcg gcaaaaaaca ccgaatgccg 2220

ccatacagga caccgtactg agcgtctgca actctgcatc ccgctcactg ctcacatctc 2280

cgcttgccgc ctcacccatc cgactcagac cagagccaca cggattactg ctgctggtgt 2340

gtgtattaac aaaagatcca tttgaccgga gcacatgcag cttggatgga aaaaatttat 2400

tatattcgtc agtgctgcat atgtactcat acttgcatga tggttttatt tattcgacct 2460

catcagtcct ggcactatgg aaagtcattg tagtatagat tttttaatat aatataaatc 2520

attggtgact tatcttgctt aattttattt tcttattatg aaatatcgtt gcattcataa 2580

tagcaaattt gtgcaaatat atagaatcta cgtgaaattc ttggttggac caatacaaca 2640

aacccctcaa acattctctt gtactgaacc ataccattcc gtacaaccat ccaaacaaaa 2700

atcatgtatc atcatgtaca tgtaaccaaa caattaacac gcaccatcct attcagactt 2760

gtctcatcca taatctatcc atccaggatg atccatccca ttcatctata tacacccaat 2820

caaacgctac ctaaaatttg gatctgtatg tgtcacatat atcttaataa gatggatgga 2880

aatatctctt tatcttttag atatggatag gtatatatgt tgctgtgggt ttgttagtta 2940

tatatatacg tgcttacata cgtgaagaaa cctgctgcta cagtttaata attcttgttc 3000

3060

tatatacatg cttacataca tgaagaacac atgctacagt tcaaaaattc ttgttcatct 3120

cataaacaaa aaggaggtgt atatgttgct gtgggtttta ctggtacttt attagatata 3180

tacatgctta catagatgaa gcaacatgct gctatggtgt ttaataatta ttgtttatct 3240

aataaacaaa catgcttttt aattatcttg atatgtttgg atgatggcat atgcagcagc 3300

3360

ttatttgctt gagactgttt cttttgttga tactcatcct ttagttcggt cactcttctg 3420

caggtg 3426

<210> 71

<211> 1447

<212> DNA

<213> Miscanthus sinesis

<400> 71

gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa 60

attacattttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt 120

gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct 180

gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt 240

ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga 300

360

gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt 420

taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca 480

ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc 540

atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc 600

660

gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg 720

gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac 780

tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa 840

tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc 900

ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc 960

1020

gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg 1080

taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa cggcgaacc 1140

accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg 1200

gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac 1260

ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc 1320

1380

ccgctccgtc ccttttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt 1440

ctctcgt 1447

<210> 72

<211> 2878

<212> DNA

<213> Miscanthus sinesis

<400> 72

ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc 60

gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta 120

cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa 180

acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg 240

ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac 300

aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa 360

aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat 420

480

tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa 540

atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc 600

cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca 660

acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct 720

ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac 780

ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg 840

tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgtg 900

ttgttcggag cgcacacaca acccgatccc caatcaatcg atccccgctt caaggtacgg 960

cgatcctcct ccctctctct ctaccttctc ttctctacac tagatcggcg gtccatggtt 1020

agggcctgct agttccgttc ctgtttttcc atggctgcga ggtacaatag atctgatggc 1080

1140

cggtgatcca tggttagtac cctaggctgt ggagtcgggt tagatccgcg ctgttagggt 1200

tcgtatatgg aggcgagctg ttctgattgt taacttgctg ggaatcctgg gatggttcta 1260

gctgttccgc agatgagatc gatttcatga tctgctgtat ctatccgtgg tatgatgtta 1320

1380 gcctttgata tggttcgatc gtgctagcta cgtcctgtgc

tttttactat actttttttt tggtttggtt tggtttcgtc tgatttggct gtcgttctag 1440

atcagagtag aaactgtttc aaactacctg ttggatttat taaggtagcg tttggttcct 1500

ggtatcgaat catacacgca ccagtgcatc ttggatagcc agctggggcc cacctgtcca 1560

accgtttggt tgccggatcg aacgagtcca ttcaagaccg aaccatgcag agcaatcgaa 1620

tattctcttg tgacgctgta tcatccagtt cggcaaaaaa caccgaatgc cgccatacag 1680

gacaccgtac tgagcgtctg caactctgca tcccgctcac tgctcacatc tccgcttgcc 1740

gcctcaccca tccgactcag accagagcca cacggattac tgctgctggt gtgtgtatta 1800

acaaaagatc catttgaccg gagcacatgc agcttggatg gaaaaaattt attatattcg 1860

tcagtgctgc atatgtactc atacttgcat gatggtttta tttattcgac ctcatcagtc 1920

ctggcactat ggaaagtcat tgtagtatag attttttaat ataatataaa tcattggtga 1980

cttatcttgc ttaattttat ttttcttatta tgaaatatcg ttgcattcat aatagcaaat 2040

ttgtgcaaat atatagaatc tacgtgaaat tcttggttgg accaatacaa caaacccctc 2100

aaacattctc ttgtactgaa ccataccatt ccgtacaacc atccaaacaa aaatcatgta 2160

tcatcatgta catgtaacca aacaattaac acgcaccatc ctattcagac ttgtctcatc 2220

cataatctat ccatccagga tgatccatcc cattcatcta tatacaccca atcaaacgct 2280

acctaaaatt tggatctgta tgtgtcacat atatcttaat aagatggatg gaaatatctc 2340

tttatctttt agatatggat aggtatatat gttgctgtgg gtttgttagt tatatatata 2400

2460

aaataacgat aggcgtatat gttgctgtgt tttttactgg tactttgtta gatatataca 2520

tgcttacata catgaagaac acatgctaca gttcaaaaat tcttgttcat ctcataaaca 2580

aaaaggaggt gtatatgttg ctgtgggttt tactggtact ttattagata tatacatgct 2640

tacatagatg aagcaacatg ctgctatggt gtttaataat tattgtttat ctaataaaca 2700

aacatgcttt ttaattatct tgatatgttt ggatgatggc atatgcagca gctatgtgtg 2760

gattttaaat acccagcatc atgagcatgc atgaccctgc cttagtatgc agttatttgc 2820

ttgagactgt ttcttttgtt gatactcatc ctttagttcg gtcactcttc tgcaggtg 2878

<210> 73

<211> 899

<212> DNA

<213> Miscanthus sinesis

<400> 73

ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc 60

gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta 120

cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa 180

acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg 240

ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac 300

aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa 360

aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat 420

480

tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa 540

atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc 600

cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca 660

acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct 720

ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac 780

ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg 840

tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgt 899

<210> 74

<211> 2670

<212> DNA

<213> Miscanthus sinesis

<400> 74

gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat 60

tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat 120

cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg 180

ttttggtggt ttttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga 240

ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca 300

aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt 360

ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg 420

cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga 480

gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt 540

ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc 600

660

taaatagcca gccccatccc tcgtctctcg tgttgttcgg agcgcacaca caacccgatc 720

cccaatcaat cgatccccgc ttcaaggtac ggcgatcctc ctccctctct ctctaccttc 780

tcttctctac actagatcgg cggtccatgg ttagggcctg ctagttccgt tcctgttttt 840

ccatggctgc gaggtacaat agatctgatg gcgttatgat ggttaacttg tcatgctttt 900

gcgatttata gtccctttag atagttcgag atcggtgatc catggttagt accctaggct 960

gtggagtcgg gttagatccg cgctgttagg gttcgtatat ggaggcgagc tgttctgatt 1020

gttaacttgc tgggaatcct gggatggttc tagctgttcc gcagatgaga tcgatttcat 1080

gatctgctgt atctatccgt ggtatgatgt tagcctttga tatggttcga tcgtgctagc 1140

1200 tacgtcctgt gcacttaatt gtcaggtcat

tttggtttcg tctgatttgg ctgtcgttct agatcagagt agaaactgtt tcaaactacc 1260

1320

tcttggatag ccagctgggg cccacctgtc caaccgtttg gttgccggat cgaacgagtc 1380

cattcaagac cgaaccatgc agagcaatcg aatattctct tgtgacgctg tatcatccag 1440

ttcggcaaaa aacaccgaat gccgccatac aggacaccgt actgagcgtc tgcaactctg 1500

catcccgctc actgctcaca tctccgcttg ccgcctcacc catccgactc agaccagagc 1560

cacacggatt actgctgctg gtgtgtgtat taacaaaaga tccatttgac cggagcacat 1620

gcagcttgga tggaaaaaat ttattatatt cgtcagtgct gcatatgtac tcatacttgc 1680

atgatggttt tatttattcg acctcatcag tcctggcact

agatttttta atataatata aatcattggt gacttatctt gcttaatttt attttcttat 1800

tatgaaatat cgttgcattc ataatagcaa atttgtgcaa atatatagaa tctacgtgaa 1860

attcttggtt ggaccaatac aacaaacccc tcaaacattc tcttgtactg aaccatacca 1920

ttccgtacaa ccatccaaac aaaaatcatg tatcatcatg tacatgtaac caaacaatta 1980

acacgcacca tcctattcag acttgtctca tccataatct atccatccag gatgatccat 2040

tttggatctg tatgtgtcac 2100

2160

atgttgctgt gggtttgtta gttatatata tacgtgctta catacgtgaa gaaacctgct 2220

gctacagttt aataattctt gttcatctca acaaataacg ataggcgtat atgttgctgt 2280

gttttttact ggtactttgt tagatatata catgcttaca tacatgaaga acacatgcta 2340

cagttcaaaa attcttgttc atctcataaa caaaaaggag gtgtatatgt tgctgtgggt 2400

tttactggta ctttattaga tatatacatg cttacataga tgaagcaaca tgctgctatg 2460

2520

ttggatgatg gcatatgcag cagctatgtg tggattttaa atacccagca tcatgagcat 2580

gcatgaccct gccttagtat gcagttattt gcttgagact gtttcttttg ttgatactca 2640

tcctttagtt cggtcactct tctgcaggtg 2670

<210> 75

<211> 691

<212> DNA

<213> Miscanthus sinesis

<400> 75

gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat 60

tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat 120

cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg 180

ttttggtggt ttttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga 240

ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca 300

aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt 360

ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg 420

cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga 480

gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt 540

ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc 600

660

taaatagcca gccccatccc tcgtctctcg t 691

<210> 76

<211> 2485

<212> DNA

<213> Miscanthus sinesis

<400> 76

60

gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg 120

accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac 180

ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg 240

ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc 300

gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc 360

ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc 420

acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat 480

agccagcccc atccctcgtc tctcgtgttg ttcggagcgc acacacaacc cgatccccaa 540

tcaatcgatc cccgcttcaa ggtacggcga tcctcctccc tctctctcta ccttctcttc 600

tctacactag atcggcggtc catggttagg gcctgctagt tccgttcctg tttttccatg 660

gctgcgaggt acaatagatc tgatggcgtt atgatggtta acttgtcatg cttttgcgat 720

ttatagtccc tttagatagt tcgagatcgg tgatccatgg ttagtaccct aggctgtgga 780

gtcgggttag atccgcgctg ttagggttcg tatatggagg cgagctgttc tgattgttaa 840

cttgctggga atcctgggat ggttctagct gttccgcaga tgagatcgat ttcatgatct 900

gctgtatcta tccgtggtat gatgttagcc tttgatatgg ttcgatcgtg ctagctacgt 960

1020

1080

gatttattaa ggtagcgttt ggttcctggt atcgaatcat acacgcacca gtgcatcttg 1140

gatagccagc tggggcccac ctgtccaacc gtttggttgc cggatcgaac gagtccattc 1200

aagaccgaac catgcagagc aatcgaatat tctcttgtga cgctgtatca tccagttcgg 1260

caaaaaacac cgaatgccgc catacaggac accgtactga gcgtctgcaa ctctgcatcc 1320

cgctcactgc tcacatctcc gcttgccgcc tcacccatcc gactcagacc agagccacac 1380

ggattactgc tgctggtgtg tgtattaaca aaagatccat ttgaccggag cacatgcagc 1440

ttggatggaa aaaatttatt atattcgtca gtgctgcata tgtactcata cttgcatgat 1500

ggttttattt attcgacctc atcagtcctg gcactatgga aagtcattgt agtatagatt 1560

ttttaatata atataaatca ttggtgactt atcttgctta attttatttt cttattatga 1620

aatatcgttg cattcataat agcaaatttg tgcaaatata tagaatctac gtgaaattct 1680

tggttggacc aatacaacaa acccctcaaa cattctcttg tactgaacca taccattccg 1740

tacaaccatc caaacaaaaa tcatgtatca tcatgtacat gtaaccaaac aattaacacg 1800

caccatccta ttcagacttg tctcatccat aatctatcca tccaggatga tccatcccat 1860

tcatctatat acacccaatc aaacgctacc taaaatttgg atctgtatgt gtcacatata 1920

tcttaataag atggatggaa atatctcttt atcttttaga tatggatagg tatatatgtt 1980

gctgtgggtt tgttagttat atatatacgt gcttacatac gtgaagaaac ctgctgctac 2040

agtttaataa ttcttgttca tctcaacaaa taacgatagg cgtatatgtt gctgtgtttt 2100

ttactggtac tttgttagat atatacatgc ttacatacat gaagaacaca tgctacagtt 2160

2220

tggtacttta ttagatatat acatgcttac

2340

tgatggcata tgcagcagct atgtgtggat tttaaatacc cagcatcatg agcatgcatg 2400

accctgcctt agtatgcagt tatttgcttg agactgtttc ttttgttgat actcatcctt 2460

tagttcggtc actcttctgc aggtg 2485

<210> 77

<211> 506

<212> DNA

<213> Miscanthus sinesis

<400> 77

60

gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg 120

accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac 180

ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg 240

ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc 300

gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc 360

ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc 420

acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat 480

agccagcccc atccctcgtc tctcgt 506

<210> 78

<211> 4079

<212> DNA

<213> Schizachyium scoparium

<400> 78

ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag 60

agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga 120

gtgaattgat aaattttattc taggtgacat ggccttaacg atggccagta cgtggttaaa 180

tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca 240

ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt 300

360

gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct 420

gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc 480

atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta 540

catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt 600

660

ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat 720

tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt 780

840

tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc 900

cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat 960

tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa 1020

catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa 1080

catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca 1140

aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac 1200

1260

1320

tctttctta ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca 1380

tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga 1440

1500

agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca 1560

1620

gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta 1680

tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac 1740

atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg 1800

cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt 1860

tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat 1920

tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt 1980

ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt 2040

aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa 2100

gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg 2160

atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag 2220

ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa 2280

cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg 2340

aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc 2400

ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag 2460

atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat 2520

taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt 2580

atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga 2640

caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt 2700

ccgcttcctc ctccgacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt 2760

2820

caacttcttt ccccaacctc atcttttgtt cggagcacgc acacaatccg atcgatcccc 2880

aatcccctcg tctctcctcg cgagcctcgt cgatccgcca ttcaaggtac ggcgatcatc 2940

ctccctccct ctctacctgc tcttctgtag atcggcgacc ccatccatgg ttagggcctg 3000

ctagttctgt tcctgttttt tttccatggc tgcgaggtag aatagatctg atggcgttat 3060

gatggttaat ttgtcatact cttgcggtct atgggtccct ttaggtcatc aatttaattt 3120

tgggtggttg agatcggtga tccatggtta gtaccctagt cagtggggtt ggatccgtgc 3180

tattagggtt cgtagatgga ttctgatggc tcagtaactg ggaatcctag gatggttcca 3240

tctggtttgc agatgagaac gatttcatca tctgctatat cttgtttcgt tgcgtaggtt 3300

catcatctgc 3360

tatatcttgt ttcgttgcgt aggttctgtt taaactaatc cgtggtatga tgttagcctt 3420

tgataaggtt tgattgtgct agctacgtcc tgtgcagcag ttaattgtca ggtcatacgt 3480

cataattttt agcatgtctg ttttgtttg atttcgttgt ctgattaggc tgtagatagt 3540

ttcgatctac ctgtcggttt attttattaa aatttggatc cgtatgtgtg tcacatatat 3600

cttcatgatt aagatggagt tatatgggta ggttatacat gtggctgtgg atcatgatta 3660

agatggattg aagtatctct ttatctttta gttaggatag attattatat atgttgctgt 3720

3780

ttacagttta atagttcttg attatctaat aaacaaataa ggataggtat atttgttgct 3840

gttggtttta ctggtactct attagatagt actttgacat gaagcaacat cctgctatgg 3900

3960

cttggatgat gtcatatgca gcagctattt gtgaattttt cggccgtatc ttcatattgc 4020

ttgggactgt ttctttggtt gataactcac cctgttgttt ggtgatcctt ctgcaggtg 4079

<210> 79

<211> 2831

<212> DNA

<213> Schizachyium scoparium

<400> 79

ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag 60

agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga 120

gtgaattgat aaattttattc taggtgacat ggccttaacg atggccagta cgtggttaaa 180

tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca 240

ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt 300

360

gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct 420

gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc 480

atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta 540

catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt 600

660

ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat 720

tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt 780

840

tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc 900

cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat 960

tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa 1020

catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa 1080

catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca 1140

aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac 1200

1260

1320

tctttctta ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca 1380

tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga 1440

1500

agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca 1560

1620

gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta 1680

tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac 1740

atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg 1800

cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt 1860

tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat 1920

tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt 1980

ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt 2040

aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa 2100

gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg 2160

atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag 2220

ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa 2280

cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg 2340

aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc 2400

ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag 2460

atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat 2520

taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt 2580

atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga 2640

caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt 2700

ccgcttcctc ctccgacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt 2760

2820

caacttcttt c 2831

<210> 80

<211> 95

<212> DNA

<213> Schizachyium scoparium

<400> 80

cccaacctca tcttttgttc ggagcacgca cacaatccga tcgatcccca atccctcgt 60

ctctcctcgc gagcctcgtc gatccgccat tcaag 95

<210> 81

<211> 1153

<212> DNA

<213> Schizachyium scoparium

<400> 81

gtacggcgat catcctccct ccctctctac ctgctcttct gtagatcggc gaccccatcc 60

atggttaggg cctgctagtt ctgttcctgt ttttttttcca tggctgcgag gtagaataga 120

tctgatggcg ttatgatggt taatttgtca tactcttgcg gtctatggggt ccctttaggt 180

catcaattta attttgggtg gttgagatcg gtgatccatg gttagtaccc tagtcagtgg 240

ggttggatcc gtgctattag ggttcgtaga tggattctga tggctcagta actgggaatc 300

ctaggatggt tccatctggt ttgcagatga gaacgatttc atcatctgct atatcttgtt 360

tcgttgcgta ggttctgttt aaactaatcc gtggtatgat gttagccttt gataaggttg 420

atttcatcat ctgctatatc ttgtttcgtt gcgtaggttc tgtttaaact aatccgtggt 480

atgatgttag cctttgataa ggtttgattg tgctagctac gtcctgtgca gcagttaatt 540

gtcaggtcat acgtcataat ttttagcatg tctgtttttg tttgatttcg ttgtctgatt 600

aggctgtaga tagtttcgat ctacctgtcg gtttatttta ttaaaatttg gatccgtatg 660

tgtgtcacat atatcttcat gattaagatg gagttatatg ggtaggttat acatgtggct 720

gtggatcatg attaagatgg attgaagtat ctctttatct tttagttagg atagattatt 780

atatatgttg ctgttgattt tattggttct ttattatata tattcatgct tatatacata 840

aaagcaatgt gctattacag tttaatagtt cttgattatc taataaacaa ataaggatag 900

gtatatttgt tgctgttggt tttactggta ctctattaga tagtactttg acatgaagca 960

acatcctgct atggattaat aattattctt cgtctaataa aaagcatggt ttttaattat 1020

tttgatttga tatacttgga tgatgtcata tgcagcagct atttgtgaat ttttcggccg 1080

1140

ccttctgcag gtg 1153

<210> 82

<211> 3281

<212> DNA

<213> Schizachyium scoparium

<400> 82

gtggacgtta atcggtaatt cttcatttta ttttattttg ttgtcaccgc gtacatttgg 60

gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata 120

gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat 180

tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa 240

ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa 300

tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt 360

ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat 420

cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg 480

cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat 540

tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact 600

gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag 660

aaacaacaaa atttggtttt ttttgtttg gtttcgatta tagccgaact aaccaaattt 720

aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt 780

caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat 840

tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag 900

tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc 960

agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt 1020

1080

gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc 1140

ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga 1200

gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt 1260

catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa 1320

atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat 1380

1440

aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa 1500

cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca 1560

cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc 1620

tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga 1680

aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt 1740

ctcgtaaagt acattcaaac tgtgtaatta gttatttttat ttatctacat ttaatactctct 1800

1860

caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga 1920

cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt 1980

ttcccttcat cgcccgcagc tataaatagc cacccccgtc cgcaacttct ttccccaacc 2040

tcatcttttg ttcggagcac gcacacaatc cgatcgatcc ccaatcccct cgtctctcct 2100

cgcgagcctc gtcgatccgc cattcaaggt acggcgatca tcctccctcc ctctctacct 2160

gctcttctgt agatcggcga ccccatccat ggttagggcc tgctagttct gttcctgttt 2220

tttttccatg gctgcgaggt agaatagatc tgatggcgtt atgatggtta atttgtcata 2280

2340

gatccatggt tagtacccta gtcagtgggg ttggatccgt gctattaggg ttcgtagatg 2400

gattctgatg gctcagtaac tgggaatcct aggatggttc catctggttt gcagatgaga 2460

acgatttcat catctgctat atcttgtttc gttgcgtagg ttctgtttaa actaatccgt 2520

ggtatgatgt tagcctttga taaggttgat ttcatcatct gctatatctt gtttcgttgc 2580

2640

ctagctacgt cctgtgcagc agttaattgt caggtcatac gtcataattt ttagcatgtc 2700

tgtttttgtt tgatttcgtt gtctgattag gctgtagata gtttcgatct acctgtcggt 2760

ttattttatt aaaatttgga tccgtatgtg tgtcacatat atcttcatga ttaagatgga 2820

gttatatggg taggttatac atgtggctgt ggatcatgat taagatggat tgaagtatct 2880

ctttatcttt tagttaggat agattattat atatgttgct gttgatttta ttggttcttt 2940

attatatata ttcatgctta tatacataaa agcaatgtgc tattacagtt taatagttct 3000

tgattatcta ataaacaaat aaggataggt atatttgttg ctgttggttt tactggtact 3060

ctattagata gtactttgac atgaagcaac atcctgctat ggattaataa ttattcttcg 3120

3180

3240

ttgataactc accctgttgt ttggtgatcc ttctgcaggt g 3281

<210> 83

<211> 2033

<212> DNA

<213> Schizachyium scoparium

<400> 83

gtggacgtta atcggtaatt cttcatttta ttttattttg ttgtcaccgc gtacatttgg 60

gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata 120

gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat 180

tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa 240

ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa 300

tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt 360

ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat 420

cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg 480

cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat 540

tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact 600

gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag 660

aaacaacaaa atttggtttt ttttgtttg gtttcgatta tagccgaact aaccaaattt 720

aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt 780

caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat 840

tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag 900

tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc 960

agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt 1020

1080

gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc 1140

ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga 1200

gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt 1260

catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa 1320

atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat 1380

1440

aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa 1500

cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca 1560

cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc 1620

tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga 1680

aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt 1740

ctcgtaaagt acattcaaac tgtgtaatta gttatttttat ttatctacat ttaatactctct 1800

1860

caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga 1920

cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt 1980

ttcccttcat cgcccgcagc tataaaatagc cacccccgtc cgcaacttct ttc 2033

<210> 84

<211> 2294

<212> DNA

<213> Schizachyium scoparium

<400> 84

gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag 60

tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac 120

180

ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt 240

tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt 300

ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat 360

420

ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag 480

taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg 540

ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca 600

tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc 660

caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc 720

caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa 780

ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga 840

ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt 900

ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca 960

gcacggggat tcctttccca ccgctccttc ctttttccctt catcgcccgc agctataaat 1020

agccaccccc gtccgcaact tctttcccca acctcatctt ttgttcggag cacgcacaca 1080

1140

ggtacggcga tcatcctccc tccctctcta cctgctcttc tgtagatcgg cgaccccatc 1200

catggttagg gcctgctagt tctgttcctg ttttttttcc atggctgcga ggtagaatag 1260

atctgatggc gttatgatgg ttaatttgtc atactcttgc ggtctatgggg tccctttagg

1380

gggttggatc cgtgctatta gggttcgtag atggattctg atggctcagt aactgggaat 1440

cctaggatgg ttccatctgg tttgcagatg agaacgattt catcatctgc tatatcttgt 1500

1560

gatttcatca tctgctatat cttgtttcgt tgcgtaggtt ctgtttaaac taatccgtgg 1620

1680

1740

taggctgtag atagtttcga tctacctgtc ggtttatttt attaaaattt ggatccgtat 1800

gtgtgtcaca tatatcttca tgattaagat ggagttatat gggtaggtta tacatgtggc 1860

tgtggatcat gattaagatg gattgaagta tctctttatc ttttagttag gatagattat 1920

tatatatgtt gctgttgatt ttattggttc ttttattatat atattcatgc ttatatacat 1980

aaaagcaatg tgctattaca gtttaatagt tcttgattat ctaataaaca aataaggata 2040

ggtatatttg ttgctgttgg ttttactggt actctattag atagtacttt gacatgaagc 2100

aacatcctgc tatggattaa taattattct tcgtctaata aaaagcatgg tttttaatta 2160

ttttgatttg atatacttgg atgatgtcat atgcagcagc tatttgtgaa tttttcggcc 2220

gtatcttcat attgcttggg actgtttctt tggttgataa ctcaccctgt tgtttggtga 2280

tccttctgca ggtg 2294

<210> 85

<211> 1046

<212> DNA

<213> Schizachyium scoparium

<400> 85

gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag 60

tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac 120

180

ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt 240

tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt 300

ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat 360

420

ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag 480

taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg 540

ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca 600

tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc 660

caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc 720

caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa 780

ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga 840

ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt 900

ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca 960

gcacggggat tcctttccca ccgctccttc ctttttccctt catcgcccgc agctataaat 1020

agccaccccc gtccgcaact tctttc 1046

<210> 86

<211> 1795

<212> DNA

<213> Schizachyium scoparium

<400> 86

gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg 60

tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct 120

ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc 180

tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg 240

ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct 300

acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat 360

ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc 420

ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc 480

accgctcctt ccttttccct tcatcgcccg cagctataaa tagccacccc cgtccgcaac 540

ttctttcccc aacctcatct tttgttcgga gcacgcacac aatccgatcg atccccaatc 600

ccctcgtctc tcctcgcgag cctcgtcgat ccgccattca aggtacggcg atcatcctcc 660

ctccctctct acctgctctt ctgtagatcg gcgaccccat ccatggttag ggcctgctag 720

ttctgttcct gttttttttc catggctgcg aggtagaata gatctgatgg cgttatgatg 780

gttaatttgt catactcttg cggtctatgg gtccctttag gtcatcaatt taattttggg 840

tggttgagat cggtgatcca tggttagtac cctagtcagt ggggttggat ccgtgctatt 900

agggttcgta gatggattct gatggctcag taactgggaa tcctaggatg gttccatctg 960

gtttgcagat gagaacgatt tcatcatctg ctatatcttg tttcgttgcg taggttctgt 1020

ttaaactaat ccgtggtatg atgttagcct ttgataaggt tgatttcatc atctgctata 1080

1140

aaggtttgat tgtgctagct acgtcctgtg cagcagttaa ttgtcaggtc atacgtcata 1200

1260

atctacctgt cggtttattt tattaaaatt tggatccgta tgtgtgtcac atatatcttc 1320

1380

ggattgaagt atctctttat cttttagtta ggatagatta ttatatatgt tgctgttgat 1440

tttattggtt ctttattata tatattcatg cttatataca taaaagcaat gtgctattac 1500

agtttaatag ttcttgatta tctaataaac aaataaggat aggtatattt gttgctgttg 1560

gttttactgg tactctatta gatagtactt tgacatgaag caacatcctg ctatggatta 1620

ataattattc ttcgtctaat aaaaagcatg gtttttaatt attttgattt gatatacttg 1680

gatgatgtca tatgcagcag ctatttgtga atttttcggc cgtatcttca tattgcttgg 1740

gactgtttct ttggttgata actcaccctg ttgtttggtg atccttctgc aggtg 1795

<210> 87

<211> 547

<212> DNA

<213> Schizachyium scoparium

<400> 87

gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg 60

tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct 120

ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc 180

tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg 240

ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct 300

acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat 360

ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc 420

ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc 480

accgctcctt ccttttccct tcatcgcccg cagctataaa tagccacccc cgtccgcaac 540

ttctttc 547

<210> 88

<211> 3357

<212> DNA

<213> Sorghastrum nutans

<400> 88

gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt 60

gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc 120

cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt 180

tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt 240

gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg 300

taatgtagtc cggcacggca agttagactc tgtgtgtagt gttattag ccggcctctt 360

taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt 420

caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa 480

agttatatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat 540

agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt 600

660

gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg 720

ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata 780

tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat 840

atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt 900

gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcgggg ctttaagcca 960

aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata 1020

cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga 1080

gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat 1140

caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc 1200

aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag 1260

1320

1380

tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact 1440

atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta 1500

caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg 1560

agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa 1620

tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa 1680

ctatattttc atagtatact tatttaatgt tataaattttt tataattttt tttataattt 1740

tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc 1800

aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa 1860

ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc 1920

tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt 1980

ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc 2040

gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac 2100

acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct 2160

ctccttcggt ccctccctcca tcataaatag ccacccccct cccaccttct ttccccacct 2220

cgtctcccct cgtgttattc ggagcacaga cacaccccga tccccaatcc tctcctcgcg 2280

agcctcgtcg atccccgctt caaggtacgg cgatcatcct ccctccctaa ctccaatccg 2340

tggttagggc ctgctagatc gtcctccctc cctacctgcg atccgtggtt cgcgcctgct 2400

agttctgttt cctgtttgtc gatggctgcg aggtataata gatctgatgg cgtgcggtgt 2460

gacggttaaa ttcacatgct cttgcgattt atacgcgaat cgatctggga ttgctcgaga 2520

tcggtgatcc atggttagaa ccctaggcgg tggagtcggg ttaaatccgt gctgttaggg 2580

ttcgtaggtg gatgcgacct gttctggttg tttacttgtc agtatttagg aatcctacta 2640

ggatggttct agctggttcg cagatgagat cgatttcatg atctgctata tctttcgttg 2700

2760

ctagctacct cctgtgcact aaattatcag ctcgtaattt ttagcatgcc cttttttttt 2820

tgggtattgt tcgattgagg tgtcgttcta gatcagagta ggaagactgt ttcaaactac 2880

2940

ggatggaaat atcccttttt cttttagata ctgtttggta tagattttgc tgtgggtttt 3000

actggtactt agatactctt cgtttagata tggatatgtt tacatgcaga tacatgaagc 3060

aacatgctgc tacagtttaa tatggatagg tgtatatgtt gttgtgggtc ctttacttac 3120

atgcttagat acatgaagca acatgctgct acgtttaata

3240

ctatgtgtgg attttaaata cccagcatga gcatgcatga ccctaactta gtatgctgtt 3300

3357

<210> 89

<211> 2218

<212> DNA

<213> Sorghastrum nutans

<400> 89

gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt 60

gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc 120

cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt 180

tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt 240

gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg 300

taatgtagtc cggcacggca agttagactc tgtgtgtagt gttattag ccggcctctt 360

taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt 420

caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa 480

agttatatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat 540

agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt 600

660

gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg 720

ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata 780

tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat 840

atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt 900

gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcgggg ctttaagcca 960

aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata 1020

cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga 1080

gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat 1140

caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc 1200

aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag 1260

1320

1380

tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact 1440

atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta 1500

caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg 1560

agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa 1620

tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa 1680

ctatattttc atagtatact tatttaatgt tataaattttt tataattttt tttataattt 1740

tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc 1800

aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa 1860

ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc 1920

tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt 1980

ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc 2040

gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac 2100

acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct 2160

ctccttcggt cctccctcca tcataaatag ccacccccct cccaccttct ttccccac 2218

<210> 90

<211> 86

<212> DNA

<213> Sorghastrum nutans

<400> 90

ctcgtctccc ctcgtgttat tcggagcaca gacacacccc gatccccaat cctctcctcg 60

cgagcctcgt cgatccccgc ttcaag 86

<210> 91

<211> 1053

<212> DNA

<213> Sorghastrum nutans

<400> 91

gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc 60

tccctcccta cctgcgatcc gtggttcgcg

gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg 180

cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct 240

aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc 300

360

tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg 420

tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat 480

tatcagctcg taatttttag catgcccttt tttttttgggg tattgttcga ttgaggtgtc 540

gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat 600

ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt 660

tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt 720

tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg 780

gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat 840

gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat 900

tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca 960

gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt 1020

gatactcacc cttttgtttg ttgactcttg cag 1053

<210> 92

<211> 3106

<212> DNA

<213> Sorghastrum nutans

<400> 92

agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc 60

acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa 120

tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg 180

atataatata caagacaag tatacaataa agtgacatta gcgtaaagtt atatgacata 240

tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta 300

tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat 360

aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct 420

480

ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag 540

atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc 600

ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg 660

caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc 720

tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag 780

840

tctctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct 900

aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt 960

1020

ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga 1080

accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt 1140

tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa 1200

tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca 1260

acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag 1320

1380

aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag 1440

1500

atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga 1560

gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag 1620

ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc 1680

catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc 1740

1800

agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg 1860

aaccgtgacg gcaccggggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc 1920

ccctccatcat aaatagccac ccccctccca ccttctttcc ccacctcgtc tcccctcgtg 1980

ttattcggag cacagacaca ccccgatccc caatcctctc ctcgcgagcc tcgtcgatcc 2040

ccgcttcaag gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc 2100

2160

tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca 2220

catgctcttg cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg 2280

ttagaaccct aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg 2340

cgacctgttc tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct 2400

ggttcgcaga tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt 2460

aatctgtccg tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg 2520

tgcactaaat tatcagctcg taatttttag catgcccttt ttttttttggg tattgttcga 2580

ttgaggtgtc gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt 2640

aaatttggat ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc 2700

ctttttcttt tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat 2760

actcttcgtt tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca 2820

2880

gaagcaacat gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat 2940

gctttttaat tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt 3000

taaataccca gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt 3060

ttcttttgtt gatactcacc cttttgtttg ttgactcttg caggtg 3106

<210> 93

<211> 1964

<212> DNA

<213> Sorghastrum nutans

<400> 93

agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc 60

acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa 120

tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg 180

atataatata caagacaag tatacaataa agtgacatta gcgtaaagtt atatgacata 240

tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta 300

tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat 360

aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct 420

480

ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag 540

atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc 600

ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg 660

caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc 720

tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag 780

840

tctctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct 900

aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt 960

1020

ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga 1080

accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt 1140

tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa 1200

tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca 1260

acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag 1320

1380

aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag 1440

1500

atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga 1560

gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag 1620

ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc 1680

catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc 1740

1800

agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg 1860

aaccgtgacg gcaccggggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc 1920

ccctccatcat aaatagccac ccccctccca ccttctttcc ccac 1964

<210> 94

<211> 1056

<212> DNA

<213> Sorghastrum nutans

<400> 94

gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc 60

tccctcccta cctgcgatcc gtggttcgcg

gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg 180

cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct 240

aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc 300

360

tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg 420

tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat 480

tatcagctcg taatttttag catgcccttt tttttttgggg tattgttcga ttgaggtgtc 540

gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat 600

ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt 660

tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt 720

tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg 780

gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat 840

gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat 900

tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca 960

gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt 1020

gatactcacc cttttgtttg ttgactcttg caggtg 1056

<210> 95

<211> 2165

<212> DNA

<213> Sorghastrum nutans

<400> 95

gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt 60

ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat 120

gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga 180

gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa 240

atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg 300

tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg 360

gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga 420

ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc 480

atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat 540

aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca 600

tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca 660

gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg 720

acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg 780

gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc 840

ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct 900

ctcacacggc acggaacgga accgtgacgg caccgggcag cacggggcggg attccttccc 960

cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc 1020

cacctcgtct cccctcgtgt tattcggagc acagacacac cccgatcccc aatcctctcc 1080

tcgcgagcct cgtcgatccc cgcttcaagg tacggcgatc atcctccctc cctaactcca 1140

atccgtggtt agggcctgct agatcgtcct ccctccctac ctgcgatccg tggttcgcgc 1200

ctgctagttc tgtttcctgt ttgtcgatgg ctgcgaggta taatagatct gatggcgtgc 1260

ggtgtgacgg ttaaattcac atgctcttgc gatttatacg cgaatcgatc tgggattgct 1320

1380

tagggttcgt aggtggatgc gacctgttct ggttgtttac ttgtcagtat ttaggaatcc 1440

tactaggatg gttctagctg gttcgcagat gagatcgatt tcatgatctg ctatatcttt 1500

1560

1620

ttttttgggt attgttcgat tgaggtgtcg ttctagatca gagtaggaag actgtttcaa 1680

actacctgct ggatttatta aatttggatc tgtatgagta tcacatatat ctccataatt 1740

1800

gttttactgg tacttagata ctcttcgttt agatatggat atgtttacat gcagatacat 1860

gaagcaacat gctgctacag tttaatatgg ataggtgtat atgttgttgt gggtccttta 1920

cttacatgct tagatacatg aagcaacatg ctgctacgtt taataattat tgtttatctg 1980

atctgattta aacaaacatg ctttttaatt gtcctgaaat gcttggatga tggcatatgc 2040

agcagctatg tgtggatttt aaatacccag catgagcatg catgacccta acttagtatg 2100

2160

aggtg 2165

<210> 96

<211> 1023

<212> DNA

<213> Sorghastrum nutans

<400> 96

gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt 60

ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat 120

gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga 180

gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa 240

atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg 300

tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg 360

gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga 420

ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc 480

atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat 540

aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca 600

tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca 660

gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg 720

acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg 780

gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc 840

ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct 900

ctcacacggc acggaacgga accgtgacgg caccgggcag cacggggcggg attccttccc 960

cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc 1020

cac 1023

<210> 97

<211> 1866

<212> DNA

<213> Sorghastrum nutans

<400> 97

gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac 60

ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg 120

attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt 180

catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta 240

taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac 300

atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc 360

agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg cggagacgtt 420

gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc 480

ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg 540

cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc 600

tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacggggcgg gattccttcc 660

ccacctctcc ttcggtcctc ccctccatcat aaatagccac ccccctccca ccttctttcc 720

ccacctcgtc tcccctcgtg ttattcggag cacagacaca ccccgatccc caatcctctc 780

ctcgcgagcc tcgtcgatcc ccgcttcaag gtacggcgat catcctccct ccctaactcc 840

aatccgtggt tagggcctgc tagatcgtcc tccctcccta cctgcgatcc gtggttcgcg 900

cctgctagtt ctgtttcctg tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg 960

cggtgtgacg gttaaattca catgctcttg cgatttatac gcgaatcgat ctgggattgc 1020

tcgagatcgg tgatccatgg ttagaaccct aggcggtgga gtcgggttaa atccgtgctg 1080

ttagggttcg taggtggatg cgacctgttc tggttgttta cttgtcagta tttaggaatc 1140

ctactaggat ggttctagct ggttcgcaga tgagatcgat ttcatgatct gctatatctt 1200

1260

atcgtgctag ctacctcctg tgcactaaat tatcagctcg taatttttag catgcccttt 1320

tttttttggg tattgttcga ttgaggtgtc gttctagatc agagtaggaa gactgtttca 1380

aactacctgc tggatttatt aaatttggat ctgtatgagt atcacatata tctccataat 1440

ttagatggat ggaaatatcc ctttttcttt tagatactgt ttggtataga ttttgctgtg 1500

ggttttactg gtacttagat actcttcgtt tagatatgga tatgtttaca tgcagataca 1560

1620

acttacatgc ttagatacat gaagcaacat gctgctacgt ttaataatta ttgtttatct 1680

gatctgattt aaacaaacat gctttttaat tgtcctgaaa tgcttggatg atggcatatg 1740

cagcagctat gtgtggattt taaataccca gcatgagcat gcatgaccct aacttagtat 1800

1860

1866

<210> 98

<211> 724

<212> DNA

<213> Sorghastrum nutans

<400> 98

gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac 60

ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg 120

attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt 180

catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta 240

taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac 300

atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc 360

agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg cggagacgtt 420

gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc 480

ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg 540

cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc 600

tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacggggcgg gattccttcc 660

ccacctctcc ttcggtcctc ccctccatcat aaatagccac ccccctccca ccttctttcc 720

ccc 724

<210> 99

<211> 2625

<212> DNA

<213> Setaria italica

<400> 99

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg 1500

caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg 1560

tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg 1620

tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt 1680

gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc 1740

agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa 1800

tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac 1860

ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc 1920

ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc 1980

2040

agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct 2100

gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg 2160

aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta 2220

gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct 2280

agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc 2340

gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg 2400

aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac 2460

atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt 2520

2580

tgtttgcaag ctttctgaca ttattctatt gttctgaaac agggt 2625

<210> 100

<211> 1492

<212> DNA

<213> Setaria italica

<400> 100

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc 1492

<210> 101

<211> 127

<212> DNA

<213> Setaria italica

<400> 101

cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt 60

ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc 120

cagcaag 127

<210> 102

<211> 1006

<212> DNA

<213> Setaria italica

<400> 102

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240

cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt 300

cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc 360

caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag 420

tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc 480

tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg 540

gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt 600

agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc 660

tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat 720

cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag 780

gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa 840

catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg 900

ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc 960

atgtttgcaa gctttctgac attattctat tgttctgaaa cagggt 1006

<210> 103

<211> 2625

<212> DNA

<213> Setaria italica

<400> 103

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg 1500

caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg 1560

tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg 1620

tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt 1680

gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc 1740

agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa 1800

tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac 1860

ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc 1920

ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc 1980

2040

agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct 2100

gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg 2160

aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta 2220

gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct 2280

agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc 2340

gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg 2400

aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac 2460

atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt 2520

2580

tgtttgcaag ctttctgaca ttattctatt gttctgaaac aggtg 2625

<210> 104

<211> 1492

<212> DNA

<213> Setaria italica

<400> 104

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080

gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140

tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200

tggcggaaga aaggaatggc tcgtagggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260

cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320

gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380

acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440

caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc 1492

<210> 105

<211> 1006

<212> DNA

<213> Setaria italica

<400> 105

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240

cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt 300

cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc 360

caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag 420

tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc 480

tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg 540

gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt 600

agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc 660

tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat 720

cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag 780

gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa 840

catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg 900

ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc 960

atgtttgcaa gctttctgac attattctat tgttctgaaa caggtg 1006

<210> 106

<211> 2167

<212> DNA

<213> Setaria italica

<400> 106

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg 180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg 300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600

agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg 660

gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc 720

ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gcccgggtag 780

aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg 840

acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc 900

acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg 960

gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa 1020

ataccctccc atcccgttgc cgcaagactc agatcagatt ccgatcccca gttcttcccc 1080

aatcaccttg tggtctctcg tgtcgcggtt cccagggacg cctccggctc gtcgctcgac 1140

agcgatctcc gccccagcaa ggtatagatt cagttccttg ctccgatccc aatctggttg 1200

agatgttgct ccgatgcgac ttgattatgt catatatctg cggtttgcac cgatctgaag 1260

cctagggttt ctcgagcgac ccagttattt gcaatttgcg atttgctcgt ttgttgcgca 1320

gcgtagttta tgtttggagt aatcgaggat ttgtatgcgg cgtcggcgct acctgcttaa 1380

tcacgccatg tgacgcggtt acttgcagag gctgggttct gttatgtcgt gatctaagaa 1440

tctagattag gctcagtcgt tcttgctgtc gactagtttg ttttgatatc catgtagtac 1500

aagttactta aaatttaggt ccaatatatt ttgcatgctt ttggcctgtt attcttgcca 1560

1620

agtgctatag ttctatagtt ctgtgataca tctatctgat ttttttttggt ctattggtgc 1680

ctaacttatc tgaaaatcat ggaacatgag gctagtttga tcatggttta gttcattgtg 1740

attaataatg tatgatttag tagctatttt ggtgatcgtg tcattttatt tgtgaatgga 1800

atcattgtat gtaaatgaag ctagttcagg ggttacgatg tagctggctt tgtattctaa 1860

aggctgctat tattcatcca tcgatttcac ctatatgtaa tccagagctt ttgatgtgaa 1920

atttgtctga tccttcacta ggaaggacag aacattgtta atattttggc acatctgtct 1980

tattctcatc ctttgtttga acatgttagc ctgttcaaac agatactgtt gtaatgtcct 2040

agttatatag gtacatatgt gttctctatt gagtttatgg acttttgtgt gtgaagttat 2100

atttcatttt gctcaaaact catgtttgca agctttctga cattattcta ttgttctgaa 2160

acaggtg 2167

<210> 107

<211> 1034

<212> DNA

<213> Setaria italica

<400> 107

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg 180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg 300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600

agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg 660

gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc 720

ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gcccgggtag 780

aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg 840

acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc 900

acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg 960

gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa 1020

ataccctccc atcc 1034

<210> 108

<211> 1813

<212> DNA

<213> Setaria italica

<400> 108

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc 300

gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactggggc 360

caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtagggggccc 420

gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct 480

aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg 540

aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc 600

cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag 660

gcataaatac cctcccatcc cgttgccgca agactcagat cagattccga tccccagttc 720

ttccccaatc accttgtggt ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg 780

ctcgacagcg atctccgccc cagcaaggta tagattcagt tccttgctcc gatcccaatc 840

tggttgagat gttgctccga tgcgacttga ttatgtcata tatctgcggt ttgcaccgat 900

ctgaagccta gggtttctcg agcgacccag ttatttgcaa tttgcgattt gctcgtttgt 960

tgcgcagcgt agtttatgtt tggagtaatc gaggatttgt atgcggcgtc ggcgctacct 1020

gcttaatcac gccatgtgac gcggttactt gcagaggctg ggttctgtta tgtcgtgatc 1080

taagaatcta gattaggctc agtcgttctt gctgtcgact agtttgtttt gatatccatg 1140

tagtacaagt tacttaaaat ttaggtccaa tatattttgc atgcttttgg cctgttattc 1200

ttgccaacaa gttgtcctgg taaaaagtag atgtgaaagt cacgtattgg gacaaattga 1260

1320 tggtttagtg ctatagttct atagttctgt

tggtgcctaa cttatctgaa aatcatggaa catgaggcta gtttgatcat ggtttagttc 1380

1440

aatggaatca ttgtatgtaa atgaagctag ttcaggggtt acgatgtagc tggctttgta 1500

ttctaaaggc tgctattatt catccatcga tttcacctat atgtaatcca gagcttttga 1560

1620

ctgtcttatt ctcatccttt gtttgaacat gttagcctgt tcaaacagat actgttgtaa 1680

tgtcctagtt atataggtac atatgtgttc tctattgagt ttatggactt ttgtgtgtga 1740

agttatattt cattttgctc aaaactcatg tttgcaagct ttctgacatt attctattgt 1800

gtg 1813

<210> 109

<211> 680

<212> DNA

<213> Setaria italica

<400> 109

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc 300

gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactggggc 360

caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtagggggccc 420

gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct 480

aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg 540

aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc 600

cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag 660

gcataaatac cctcccatcc 680

<210> 110

<211> 2634

<212> DNA

<213> Setaria viridis

<400> 110

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg 1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc 1500

gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt 1560

gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag 1620

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 1680

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 1740

1800

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 1860

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 1920

1980

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 2040

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 2100

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 2160

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 2220

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 2280

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 2340

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 2400

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 2460

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 2520

2580

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg 2634

<210> 111

<211> 1493

<212> DNA

<213> Setaria viridis

<400> 111

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg 1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcc 1493

<210> 112

<211> 127

<212> DNA

<213> Setaria viridis

<400> 112

cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt 60

ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc 120

cagcaag 127

<210> 113

<211> 1014

<212> DNA

<213> Setaria viridis

<400> 113

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 300

360

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 420

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 480

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 540

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 600

660

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 720

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 780

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 840

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 900

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 960

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg 1014

<210> 114

<211> 2634

<212> DNA

<213> Setaria viridis

<400> 114

actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60

ggagcagcga tctggattgg agagaataga ggaaaagag ggaaaaggag agagatagcg 120

caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180

catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240

ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300

gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360

tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420

aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480

ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540

tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600

cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660

ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720

cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780

atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840

gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900

ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960

aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020

acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080

ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140

gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200

ttggcggaag aaaggaatgg ctcgtaggggg cccgggtaga atccaagaat gttgcgctgg 1260

gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320

ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380

gacgcgggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440

gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc 1500

gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt 1560

gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag 1620

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 1680

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 1740

1800

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 1860

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 1920

1980

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 2040

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 2100

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 2160

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 2220

gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 2280

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 2340

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 2400

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 2460

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 2520

2580

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt 2634

<210> 115

<211> 1014

<212> DNA

<213> Setaria viridis

<400> 115

gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60

tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120

180

atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240

cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 300

360

tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 420

gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 480

tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 540

aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 600

660

aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 720

tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 780

ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 840

tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 900

catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 960

caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt 1014

<210> 116

<211> 2176

<212> DNA

<213> Setaria viridis

<400> 116

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg 180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg 300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600

agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt 660

ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg 720

cctcgtcgca actcgcaacc cgttggcggga agaaaggaat ggctcgtagg ggcccgggta 780

gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac 840

gacggcccgg tcaccggggcg atggaaagag accggatcct cctcgtgaat tctggaaggc 900

cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg 960

ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata 1020

aataccctcc catcccgttg ccgcaagact cagatcagat tccgatcccc agttcttccc 1080

caatcacctt gtggtctctc gtgtcgcggt tcccagggac gcctccggct cgtcgctcga 1140

cagcgatctc cgccccagca aggtatagat tcagttcctt gctccgatcc caatctggtt 1200

gagatgttgc tccgatgcga cttgattatg tcatatatct gcggtttgca ccgatctgaa 1260

gcctagggtt tctcgagcga cccagttgtt tgcaatttgc gatttgctcg tttgttgcgc 1320

1380

atcacgccat gtgacgcggt tacttgcaga ggctgggtta gtgggttctg ttatgtcgtg 1440

atctaagaat ctagattagg ctcagtcgtt cttgctgtcg actagtttgt tttgatatcc 1500

tgcatgcttt tggcctgtta 1560

ttcttgccaa caagttgtcc tggtaaaaag tagatgtgaa agtcacgtat tgggacaaat 1620

tgtggttaa gtgctatagt tctatagttc tgtgatacat ctatctgatt ttttttggtc 1680

tattggtgcc taacttatct gaaaatcatg gaacatgagg ctagtttgat catggtttag 1740

1800

1860

gtattctaaa ggctgctatt attcatccat cgatttcacc tatatgtaat cgagcttt 1920

cgatgtgaaa tttgtctgat ccttcactag gaaggacaga acattgttaa tattttggca 1980

catctgtctt attctcatcc tttgtttgaa catgttagcc tgttcaaaca gatactgttg 2040

2100

2160

tgttctgaaa caggtg 2176

<210> 117

<211> 1035

<212> DNA

<213> Setaria viridis

<400> 117

gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60

acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120

aggcactagg cagagataga gccggggggtg aatggggcta aagctcagct gctcgagggg 180

ccgtgggctg gtttcacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240

agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg 300

ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360

taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420

cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480

ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540

agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600

agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt 660

ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg 720

cctcgtcgca actcgcaacc cgttggcggga agaaaggaat ggctcgtagg ggcccgggta 780

gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac 840

gacggcccgg tcaccggggcg atggaaagag accggatcct cctcgtgaat tctggaaggc 900

cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg 960

ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata 1020

aataccctcc catcc 1035

<210> 118

<211> 1822

<212> DNA

<213> Setaria viridis

<400> 118

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc 420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg 540

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660

ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt 720

cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc 780

gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat 840

ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga 900

tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg 960

ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc 1020

tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat 1080

gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg 1140

atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc 1200

1260

acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt 1320

ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg 1380

1440

ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct 1500

ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag 1560

agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt 1620

1680

ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt 1740

1800

ttctattgtt ctgaaacagg tg 1822

<210> 119

<211> 681

<212> DNA

<213> Setaria viridis

<400> 119

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc 420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg 540

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660

ggcataaata ccctccatc c 681

<210> 120

<211> 1822

<212> DNA

<213> Setaria viridis

<400> 120

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc 420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg 540

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660

ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt 720

cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc 780

gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat 840

ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga 900

tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg 960

ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc 1020

tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat 1080

gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg 1140

atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc 1200

1260

acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt 1320

ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg 1380

1440

ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct 1500

ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag 1560

agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt 1620

1680

ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt 1740

1800

ttctattgtt ctgaaacagg gt 1822

<210> 121

<211> 681

<212> DNA

<213> Setaria viridis

<400> 121

cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60

gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120

atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180

atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240

cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300

cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360

ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtagggggcc 420

cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480

taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg 540

600

ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660

ggcataaata ccctccatc c 681

<210> 122

<211> 1925

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 122

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt 780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840

ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa 900

atccaccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct 960

ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt 1020

catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat 1080

gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat 1140

1200

1260

1320

1380

tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat 1440

ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt 1500

ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac 1560

1620

atagttacga gtttaagatc gatggaaata tcgatctagg

gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga 1740

gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 1800

gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 1860

tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc 1920

aggtc 1925

<210> 123

<211> 850

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 123

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt 780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840

ctctttcccc 850

<210> 124

<211> 78

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 124

aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa atccacccgt 60

cggcacctcc gcttcaag 78

<210> 125

<211> 997

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 125

gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc 60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt 120

tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat 180

tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga 240

cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 300

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgt tttttttggc 360

ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt 420

caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt 480

acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540

ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg 600

tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta 660

attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa 720

tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat 780

atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat 840

gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt 900

ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg 960

atgctcaccc tgttgtttgg tgatacttct gcaggtc 997

<210> 126

<211> 1925

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 126

gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60

120

aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180

attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240

ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300

ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360

ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420

aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480

aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540

caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600

aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660

ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720

gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacgggg ggattccttt 780

cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840

ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa 900

atccaccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct 960

ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt 1020

catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat 1080

gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat 1140

1200

1260

1320

1380

tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat 1440

ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt 1500

ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac 1560

1620

atagttacga gtttaagatc gatggaaata tcgatctagg

gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga 1740

gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 1800

gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 1860

tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc 1920

1925

<210> 127

<211> 997

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 127

gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc 60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt 120

tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat 180

tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga 240

cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 300

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgt tttttttggc 360

ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt 420

caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt 480

acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540

ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg 600

tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta 660

attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa 720

tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat 780

atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat 840

gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt 900

ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg 960

atgctcaccc tgttgtttgg tgatacttct gcagggt 997

<210> 128

<211> 1974

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 128

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc 300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg 660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt 900

tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 960

caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt 1020

1080

ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga 1140

ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag 1200

1260

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt 1320

cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt 1380

tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat 1440

agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg 1500

1560

ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg 1620

1680

aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg 1740

catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta 1800

ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat 1860

atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg 1920

1974

<210> 129

<211> 887

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 129

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc 300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg 660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc ttttcccc 887

<210> 130

<211> 77

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 130

aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc 60

ggcacctccg cttcaag 77

<210> 131

<211> 1010

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 131

gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat 60

ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 120

atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 180

taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 240

300

360

gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa 420

actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt 480

acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540

ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg 600

ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt 660

atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga 720

tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata 780

tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat 840

aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc 900

agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact 960

gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcaggtc 1010

<210> 132

<211> 1974

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 132

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60

120

ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180

atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240

tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaa tagcttcacc 300

tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360

tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420

480

gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540

ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600

ccagcgaacc agcagcgtcg cgtcggggcca agcgaagcag acggcacggc atctctgtcg 660

ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720

tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780

ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840

ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt 900

tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 960

caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt 1020

1080

ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga 1140

ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag 1200

1260

tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt 1320

cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt 1380

tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat 1440

agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg 1500

1560

ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg 1620

1680

aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg 1740

catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta 1800

ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat 1860

atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg 1920

tactgtttct tttgtcgatg ctcaccctgt tgtttggtga tacttctgca gggt 1974

<210> 133

<211> 1010

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 133

gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat 60

ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 120

atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 180

taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 240

300

360

gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa 420

actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt 480

acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540

ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg 600

ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt 660

atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga 720

tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata 780

tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat 840

aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc 900

agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact 960

gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcagggt 1010

<210> 134

<211> 2008

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 134

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata 300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840

gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc 900

aacacaccgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg 960

ccgctcatcc tcccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg 1020

ttagggcccg gtagttctac ttctgttcat gttgtgtta gagcaaacat gttcatgttc 1080

1140

caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata 1200

1260

gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt 1320

ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt 1380

attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg 1440

acatcttcat agttacgagt ttaagatgat 1500

ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 1560

1620

atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt 1680

gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg 1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat 1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa 1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt 1920

agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc 1980

ctgttgttgg gtgatacttc tgcaggtc 2008

<210> 135

<211> 877

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 135

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata 300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840

gtaataaata gacaccccct ccacaccctc ttttcccc 877

<210> 136

<211> 78

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 136

aacctcgtgt tcgttcggag cgcacacaca cgcaaccaga tctcccccaa atccagccgt 60

cggcacctcc gcttcaag 78

<210> 137

<211> 1053

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 137

gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc 60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca 120

tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac 180

tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct 240

tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt 300

360

atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 420

gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct 480

ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag 540

atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 600

catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt 660

tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt 720

tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat 780

ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 840

ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 900

tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 960

tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc 1020

tcaccctgtt gttgggtgat acttctgcag gtc 1053

<210> 138

<211> 2008

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 138

gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaaatagctt gacctatata 300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840

gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc 900

aacacaccgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg 960

ccgctcatcc tcccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg 1020

ttagggcccg gtagttctac ttctgttcat gttgtgtta gagcaaacat gttcatgttc 1080

1140

caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata 1200

1260

gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt 1320

ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt 1380

attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg 1440

acatcttcat agttacgagt ttaagatgat 1500

ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 1560

1620

atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt 1680

gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg 1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat 1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa 1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt 1920

agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc 1980

ctgttgttgg gtgatacttc tgcaggggt 2008

<210> 139

<211> 1053

<212> DNA

<213> Zea mays subsp. Mexicana

<400> 139

gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc 60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca 120

tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac 180

tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct 240

tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt 300

360

atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 420

gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct 480

ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag 540

atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 600

catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt 660

tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt 720

tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat 780

ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 840

ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 900

tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 960

tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc 1020

tcaccctgtt gttgggtgat acttctgcag ggt 1053

<210> 140

<211> 1635

<212> DNA

<213> Sorghum bicolor

<400> 140

ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat 60

ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct 120

tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa

aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt 240

tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc 300

gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac 360

ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga cgtaaccctc cgttgcccac 420

gataaaagct ccacccccga ccccggcccc ccgatttccc ctacggacca gtctcccccc 480

gatcgcaatc gcgaattcgt cgcaccatcg gcacgcagac gaacgaagca aggctctccc 540

catcggctcg tcaaggtatg cgttccctag atttgttccc ttcctctctc ggtttgtcta 600

tatatatgca tgtatggtcg attcccgatc tcgtcgattc tcggtttcgc cttccgtacg 660

aagattcgtt tagattgttc atatgttctg ttgtgttacc agattgatcg gatcaacttg 720

atccagttat cttcgctcct ccgattagat ccgtttctat ttcagtatat atatactagt 780

atagtatcta gggttcacac tgttgaccga ctggttactt ggaattgatc cgtgctgagt 840

tcagttgttg ccgtccataa aggcccgtgc tattgtctgt tctgaaacga aatcctgtag 900

atttcttagg gttagtgttc aattcatcaa aaggttgatt agtgaattat caaatttgag 960

agggttaaat cattctcatc atgttgtctc gaatgtaatc ccaaagatat tatagactgt 1020

1080

catagaatca tgtttaggtt tccgttcaat agactagttt tatcaatata taaaattata 1140

agaagggtag ggtaaatcac gttgcctcaa atgccatcct gtatggtttg gtttcaattc 1200

aattagtttg gttgattagg gtatgctctg gattaagatg gttaaatctt ccctagcatc 1260

ttccctgcct atccttactt gatccgtttc ggatatgttg gaagtacagc gagcttattt 1320

catgttgata gtgacccctt tcagattata ctattgaata ttgtatgttt gccacttctg 1380

tatgttgaat tatcctgcta aattagcaat ggaattagca tattggcaat tggtatgcat 1440

ggacctaatc aggacggatg tggttatgtt agtttcaatt cattgtcaat tcattgttca 1500

cctgcgttag atatatatga tgatttttac gtgtagttca tagttcttga gttttggatc 1560

tttcttatct gatatatgct ttcctgtgcc tgtgctttat tgtgtcttac catgcgattt 1620

ttgtctatgc aggtc 1635

<210> 141

<211> 401

<212> DNA

<213> Sorghum bicolor

<400> 141

ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat 60

ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct 120

tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa

aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt 240

tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc 300

gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac 360

ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga c 401

<210> 142

<211> 154

<212> DNA

<213> Sorghum bicolor

<400> 142

gtaaccctcc gttgccccg ataaaagctc cacccccgac cccggccccc cgatttcccc 60

tacggaccag tctccccccg atcgcaatcg cgaattcgtc gcaccatcgg cacgcagacg 120

aacgaagcaa ggctctcccc atcggctcgt caag 154

<210> 143

<211> 1080

<212> DNA

<213> Sorghum bicolor

<400> 143

gtatgcgttc cctagatttg ttcccttcct ctctcggttt gtctatatat atgcatgtat 60

ggtcgattcc cgatctcgtc gattctcggt ttcgccttcc gtacgaagat tcgtttagat 120

tgttcatatg ttctgttgtg ttaccagatt gatcggatca acttgatcca gttatcttcg 180

ctcctccgat tagatccgtt tctatttcag tatatatata ctagtatagt atctagggtt 240

cacactgttg accgactggt tacttggaat tgatccgtgc tgagttcagt tgttgccgtc 300

cataaaggcc cgtgctattg tctgttctga aacgaaatcc tgtagatttc ttagggttag 360

tgttcaattc atcaaaaggt tgattagtga attatcaaat ttgagagggt taaatcattc 420

tcatcatgtt gtctcgaatg taatcccaaa gatattatag actgtgtttc gatttgatgg 480

attgatttgt gtatcatcta aatcaacaag gctaagtcat cagttcatag aatcatgttt 540

aggtttccgt tcaatagact agttttatca atatataaaa ttataagaag ggtagggtaa 600

atcacgttgc ctcaaatgcc atcctgtatg gtttggtttc aattcaatta gtttggttga 660

ttagggtatg ctctggatta agatggttaa atcttcccta gcatcttccc tgcctatcct 720

tacttgatcc gtttcggata tgttggaagt acagcgagct tatttcatgt tgatagtgac 780

ccctttcaga ttatactatt gaatattgta tgtttgccac ttctgtatgt tgaattatcc 840

tgctaaatta gcaatggaat tagcatattg gcaattggta tgcatggacc taatcaggac 900

ggatgtggtt atgttagttt caattcattg tcaattcatt gttcacctgc gttagatata 960

tttacgtgta gttcatagtt cttgagtttt ggatctttct tatctgatat 1020

atgctttcct gtgcctgtgc tttattgtgt cttaccatgc gatttttgtc tatgcaggtc 1080

<210> 144

<211> 2067

<212> DNA

<213> Sorghum bicolor

<400> 144

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600

ggatcgcacc atatggggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840

atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc 900

aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag 960

tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta 1020

cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg 1080

gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt 1140

1200

tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga 1260

ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta 1320

ctccagtgct atattcacat tgtttgaggc ttgagttttg tcgatcagtg gtcatgagaa 1380

aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat 1440

1500

1560

catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg 1620

1680

gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc 1740

ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat 1800

ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg 1860

ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg 1920

ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa 1980

cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag 2040

tttctttgtg tttgattgaa acaggtg 2067

<210> 145

<211> 855

<212> DNA

<213> Sorghum bicolor

<400> 145

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600

ggatcgcacc atatggggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840

atccaggcaa ggcgc 855

<210> 146

<211> 136

<212> DNA

<213> Sorghum bicolor

<400> 146

agagcctcag accagattcc gatcaatcac ccataagctc cccccaaatc tgttcctcgt 60

ctcccgtctc gcggtttcct acttccctcg gacgcctccg gcaagtcgct cgaccgcgcg 120

attccgcccg ctcaag 136

<210> 147

<211> 1076

<212> DNA

<213> Sorghum bicolor

<400> 147

gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt 60

ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg 120

aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc 180

ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc 240

tttgtagagg ctgggggtct aggctgtgat tttgaatca aataaagctg ttccttaccg 300

tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct 360

tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat 420

tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg 480

ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt 540

gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac 600

aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga 660

720

actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat 780

cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt 840

ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata 900

taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa 960

catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta 1020

aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa caggtg 1076

<210> 148

<211> 2067

<212> DNA

<213> Sorghum bicolor

<400> 148

cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60

ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120

aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180

tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240

ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300

360

gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420

attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480

cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540

gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600

ggatcgcacc atatggggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660

aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720

cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780

aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840

atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc 900

aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag 960

tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta 1020

cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg 1080

gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt 1140

1200

tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga 1260

ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta 1320

1380

aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat 1440

1500

1560

catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg 1620

1680

gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc 1740

ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat 1800

ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg 1860

ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg 1920

ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa 1980

cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag 2040

tttctttgtgtttgattgaaacagggt 2067

<210> 149

<211> 1076

<212> DNA

<213> Sorghum bicolor

<400> 149

gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt 60

ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg 120

aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc 180

ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc 240

tttgtagagg ctgggggtct aggctgtgat tttgaatca aataaagctg ttccttaccg 300

tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct 360

tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat 420

tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg 480

ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt 540

gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac 600

aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga 660

720

actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat 780

cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt 840

ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata 900

taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa 960

catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta 1020

aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa cagggt 1076

<210> 150

<211> 2003

<212> DNA

<213> Sorghum bicolor

<400> 150

agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa 60

aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata 120

agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat 180

ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat 240

gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa 300

cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc 360

cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtgggga 420

cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg 480

ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc 540

atcccctccc tgcctcatcc atccaaatcc cactccccaa tcccatcccg tcggagaaat 600

tcatcgaagc gaagcgaagc gaatcctccc gatcctctca aggtacgcga gttttcgaat 660

cccctccaga cccctcgtat gctttccctg ttcgttttcg tcgtagcgtt tgattaggta 720

tgctttccct gttcgtgttc gtcgtagggt tcgattaggt cgtgtgaggc catggcctgc 780

tgtgataaat ttatttgttg ttatatcgga tctgtagtcg atttgggggt cgtggtgtag 840

atccgcgggc tgtgatgaag ttatttggtg tgattgtgct cgcgtgattc tgcgcgttga 900

gctcgagtag atctgatggt tggacgaccg attggttcgt tggctggctg cgctaaggtt 960

gggctgggct catgttgcgt tcgctgttgc gcgtgattcc gcggatggac ttgcgcttga 1020

ttgccgccag atcacgttac gattatgtga tttcgtttgg aactttttag atttgtagct 1080

tctgcttatt atatgacaga tgcgcctact gctcatatgc ctgtggtaaa taatggatgg 1140

ctgtgggtca aactagttga ttgtcgagtc atgtatcata tacaggtgta tagacttgcg 1200

tctaattgtt tgcatgttgc agttatatga tttgttttag attgtttgtt ccactcatct 1260

1320

1380

taatcataga tcactgtagt tgattgttga atcatgtgtc aaatacccgt atacataaca 1440

ctacacattt gcttagttgt ttccttaact catgcaaatt gaacaccatg tatgatttgc 1500

atggtgctgt aatgttaaat actacagtcc tgttggtact tgtttagtaa gaatctgctt 1560

catacaacta tatgctatgc ctgatgataa tcatatatct ttgtgtaatt aataattagt 1620

tgactgttga ataatgtatc gagtacatac catggcacaa ttgcttagtc acttccttaa 1680

ccatgcatat tgaactgacc ccttcatgtt ctgctgaatt gttctattct gattagacca 1740

tacatcatgt attgcaatct ttatttgcaa ttgtaatgta atggttcggt tctcaaatgt 1800

taaatgctat agttgtgcta ctttctaatg ttaaatgcta tagctgtgct acttgtaaga 1860

tctgcttcat agtttagtta aattaggatg atgagctttg atgctgtaac tttgtttgat 1920

tatgttcata gttgatcagt ttttgttaga ctcacagtaa cttatggtct cactcttctt 1980

ctggtctttg atgtttgcag cgg 2003

<210> 151

<211> 565

<212> DNA

<213> Sorghum bicolor

<400> 151

agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa 60

aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata 120

agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat 180

ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat 240

gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa 300

cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc 360

cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtgggga 420

cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg 480

ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc 540

atcccctccc tgcctcatcc atcca 565

<210> 152

<211> 77

<212> DNA

<213> Sorghum bicolor

<400> 152

aatcccactc cccaatccca tcccgtcgga gaaattcatc gaagcgaagc gaagcgaatc 60

ctcccgatcc tctcaag 77

<210> 153

<211> 1361

<212> DNA

<213> Sorghum bicolor

<400> 153

gtacgcgagt tttcgaatcc cctccagacc cctcgtatgc ttttccctgtt cgttttcgtc 60

gtagcgtttg attaggtatg ctttccctgt tcgtgttcgt cgtagggttc gattaggtcg 120

tgtgaggcca tggcctgctg tgataaattt atttgttgtt atatcggatc tgtagtcgat 180

ttgggggtcg tggtgtagat ccgcgggctg tgatgaagtt atttggtgtg attgtgctcg 240

cgtgattctg cgcgttgagc tcgagtagat ctgatggttg gacgaccgat tggttcgttg 300

gctggctgcg ctaaggttgg gctgggctca tgttgcgttc gctgttgcgc gtgattccgc 360

ggatggactt gcgcttgatt gccgccagat cacgttacga ttatgtgatt tcgtttggaa 420

ctttttagat ttgtagcttc tgcttattat atgacagatg cgcctactgc tcatatgcct 480

gtggtaaata atggatggct gtgggtcaaa ctagttgatt gtcgagtcat gtatcatata 540

600

tgtttgttcc actcatctag gctgtaaaag ggacactact tattagcttg ttgtttaatc 660

tttttattag tagattatat tggtaatgtt ttactaatta ttattatgtt atatgtgact 720

tctgctcatg cctgattata atcatagatc actgtagttg attgttgaat catgtgtcaa 780

atacccgtat acataacact acacatttgc ttagttgttt ccttaactca tgcaaattga 840

acaccatgta tgatttgcat ggtgctgtaa tgttaaatac tacagtcctg ttggtacttg 900

tttagtaaga atctgcttca tacaactata tgctatgcct gatgataatc atatatcttt 960

gtgtaattaa taattagttg actgttgaat aatgtatcga gtacatacca tggcacaatt 1020

gcttagtcac ttccttaacc atgcatattg aactgacccc ttcatgttct gctgaattgt 1080

tctattctga ttagaccata catcatgtat tgcaatcttt atttgcaatt gtaatgtaat 1140

ggttcggttc tcaaatgtta aatgctatag ttgtgctact ttctaatgtt aaatgctata 1200

gctgtgctac ttgtaagatc tgcttcatag tttagttaaa ttaggatgat gagctttgat 1260

gctgtaactt tgtttgatta tgttcatagt tgatcagttt ttgttagact cacagtaact 1320

tatggtctca ctcttcttct ggtctttgat gtttgcagcg g 1361

<210> 154

<211> 1812

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1812)

<223> Codon-optimized coding sequence.

<400> 154

atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca 60

ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa 120

gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt 180

cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttggggca 240

ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat 300

aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg 360

tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg 420

cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac 480

ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg 540

aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg 600

tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat 660

caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac 720

ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca 780

gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag 840

ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac 900

ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg 960

attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg 1020

gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct 1080

ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc 1140

aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa 1200

aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt 1260

gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg 1320

atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt 1380

1440 gatgtgctgt gcctgaaccg ttattacggga tggtatgtcc

gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt 1500

atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg 1560

tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc 1620

agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata 1680

ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg 1740

gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga 1800

ggcaaacaat ga 1812

<210> 155

<211> 2001

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(2001)

<223> Processable intron chimeric coding sequence.

<400> 155

atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca 60

ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa 120

gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt 180

cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttggggca 240

ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat 300

aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg 360

420

taattatcat taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat 480

gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt 540

ttctaatata tgaccaaaat ttgttgatgt gcaggtatca ccgtttgtgt gaacaacgaa 600

ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag 660

cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac 720

accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt 780

aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt 840

gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg 900

aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa 960

agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag 1020

ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa 1080

gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta 1140

atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg 1200

ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt 1260

aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa 1320

gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg 1380

cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt 1440

ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg 1500

acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc 1560

gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat 1620

ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat 1680

cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac 1740

accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt 1800

1860

caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg 1920

aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg 1980

cagcagggag gcaaacaatg a 2001

<210> 156

<211> 1653

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1653)

<223> Codon-optimized coding sequence.

<400> 156

atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga 60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt 120

gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc 180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta 240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt 300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt 360

tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa 420

aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacggga ttaccaggga 480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat 540

tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga 600

tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg 660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt 720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt 780

cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac 840

aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg 900

attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg 960

aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat 1020

1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa 1140

acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt 1200

1260

ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct 1320

1380

caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt 1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat 1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata 1620

aaggccaaga aggcgggaaa gtccaaattg taa 1653

<210> 157

<211> 936

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(936)

<223> Codon-optimized coding sequence.

<400> 157

atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 60

tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 120

aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg 180

aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 240

atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 300

ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 360

tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc 420

gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag 480

gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 540

ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct 600

gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 660

cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 720

aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg 780

ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 840

gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag 900

agcttcgtgg agcgcgtgct gaagaacgag cagtaa 936

<210> 158

<211> 253

<212> DNA

<213> Agrobacterium tumefaciens

<400> 158

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 60

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 120

atgacgttat ttatgagatg ggtttttatg

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 240

atgttactag atc 253

<210> 159

<211> 210

<212> DNA

<213> Triticum aestivum

<400> 159

ctgcatgcgt ttggacgtat gctcattcag gttggagcca atttggttga tgtgtgtgcg 60

agttcttgcg agtctgatga gacatctctg tattgtgttt ctttccccag tgttttctgt 120

acttgtgtaa tcggctaatc gccaacagat tcggcgatga ataaatgaga aataaattgt 180

tctgattttg agtgcaaaaa aaaaggaatt 210

<210> 160

<211> 300

<212> DNA

<213> Oryza sativa

<400> 160

attaatcgat cctccgatcc cttaattacc ataccattac accatgcatc aatatccata 60

tatatataaa ccctttcgca cgtacttata ctatgttttg tcatacatat atatgtgtcg 120

aacgatcgat ctatcactga tatgatatga ttgatccatc agcctgatct ctgtatcttg 180

ttatttgtat accgtcaaat aaaagtttct tccacttgtg ttaataatta gctactctca 240

tctcatgaac cctatatata actagtttaa tttgctgtca attgaacatg atgatcgatg 300

<210> 161

<211> 1204

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1204)

<223> A group of chimeric transcriptional regulatory expression elements.

<400> 161

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600

catttggaga ggacacgctg acaagctgac tctagcagat cctctagaac catcttccac 660

acactcaagc cacactattg gagaacacac agggacaaca caccataaga tccaagggag 720

gcctccgccg ccgccggtaa ccaccccgcc cctctcctct ttctttctcc gttttttttt 780

ccgtctcggt ctcgatcttt ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg 840

cgccgatc ggtgcgcggg aggggcggga tctcgcggct ggggctctcg ccggcgtgga 900

tccggcccgg atctcgcggg gaatggggct ctcggatgta gatctgcgat ccgccgttgt 960

tgggggagat gatggggggt ttaaaatttc cgccgtgcta aacaagatca ggaagagggg 1020

aaaagggcac tatggtttat atttttatat atttctgctg cttcgtcagg cttagatgtg 1080

ctagatcttt ctttcttctt tttgtgggta gaatttgaat ccctcagcat tgttcatcgg 1140

tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta 1200

gaag 1204

<210> 162

<211> 1396

<212> DNA

<213> Oryza sativa

<400> 162

tcgaggtcat tcatatgctt gagaagagag tcgggatagt ccaaaataaa acaaaggtaa 60

gattacctgg tcaaaagtga aaacatcagt taaaaggtgg tataaagtaa aatatcggta 120

ataaaaggtg gcccaaagtg aaatttactc ttttctacta ttataaaaat tgaggatgtt 180

240

ggaaatgcat atctgtattt gagtcgggtt ttaagttcgt ttgcttttgt aaatacagag 300

ggatttgtat aagaaatatc tttagaaaaa cccatatgct aatttgacat aatttttgag 360

aaaaatatat attcaggcga attctcacaa tgaacaataa taagattaaa atagctttcc 420

cccgttgcag cgcatgggta ttttttctag taaaaataaa agataaactt agactcaaaa 480

catttacaaa aacaacccct aaagttccta aagcccaaag tgctatccac gatccatagc 540

aagccagcc caacccaacc caacccagcc caccccagtc cagccaactg gacaatagtc 600

tccacacccc cccactatca ccgtgagttg tccgcacgca ccgcacgtct cgcagccaaa 660

aaaaaaaaga aagaaaaaaa agaaaaagaa aaaacagcag gtgggtccgg gtcgtggggg 720

ccggaaacgc gaggaggatc gcgagccagc gacgaggccg gccctccctc cgcttccaaa 780

gaaacgcccc ccatcgccac tatatacata cccccccctc tcctcccatc cccccaaccc 840

taccaccacc accaccacca cctccacctc ctcccccctc gctgccggac gacgagctcc 900

tcccccctcc ccctccgccg ccgccgcgcc ggtaaccacc ccgcccctct cctctttctt 960

1020

aggcggcttc gtgccgccca gatcggtgcg cgggaggggc gggatctcgc ggctggctct 1080

cgcccccgtg gatccggccc ggatctcgcg gggaatgggg ctctcggatg tagatctgcg 1140

atccgccgtt gttggggccg atgatggggc ccttaaaatt tccgccgtgc taaacaagat 1200

caggaagagg ggaaaagggc actatggttt atatttttat atatttctgc tgcttcgtca 1260

ggcttagatg tgctagatct ttctttcttc tttttgtggg tagaatttaa tccctcagca 1320

ttgttcatcg gtagtttttc ttttcatgat tcgtgacaaa tgcagcctcg tgcggacgtt 1380

ttttgtaggg tagaag 1396

<210> 163

<211> 1446

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(1446)

<223> A group of chimeric transcriptional regulatory expression elements.

<400> 163

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600

catttggaga ggacacgctg acaagctgac tctagcagat ctaccgtctt cggtacgcgc 660

tcactccgcc ctctgccttt gttactgcca cgtttctctg aatgctctct tgtgtggtga 720

ttgctgagag tggtttagct ggatctagaa ttacactctg aaatcgtgtt ctgcctgtgc 780

tgattacttg ccgtcctttg tagcagcaaa atatagggac atggtagtac gaaacgaaga 840

tagacctac acagcaatac gagaaatgtg taatttggtg cttagcggta tttatttaag 900

cacatgttgg tgttataggg cacttggatt cagaagtttg ctgttaattt aggcacaggc 960

ttcatactac atgggtcaat agtatagggga ttcatattat aggcgatact ataataattt 1020

1080 gttcgtctgc agagcttatt atttgccaaa attagatatt

1140

tctctgctcc tttattgtga ccataagtca agatcagatg cacttgtttt aaatattgtt 1200

gtctgaagaa ataagtactg acagtatttt gatgcattga tctgcttgtt tgttgtaaca 1260

1320

atctaccaac tgacactata ttgcttctct ttacatacgt atcttgctcg atgccttctc 1380

cctagtgttg accagtgtta ctcacatagt ctttgctcat ttcattgtaa tgcagatacc 1440

aagcgg 1446

<210> 164

<211> 675

<212> DNA

<213> Artificial sequence

<220>

<221> misc_feature

<222> (1)..(675)

<223> A group of chimeric transcriptional regulatory expression elements.

<400> 164

ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg 60

cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg 120

ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa 180

agatggaccc ccacccga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 240

aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg 300

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 360

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 420

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 480

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 540

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 600

ttcatttgga gaggaaccat cttccacaca ctcaagccac actattggag aacacacagg 660

gacaacacac cataa 675

<210> 165

<211> 804

<212> DNA

<213> Zea mays

<400> 165

accgtcttcg gtacgcgctc actccgccct ctgcctttgt tactgccacg tttctctgaa 60

tgctctcttg tgtggtgatt gctgagagtg gtttagctgg atctagaatt acactctgaa 120

atcgtgttct gcctgtgctg attacttgcc gtcctttgta gcagcaaaat atagggacat 180

ggtagtacga aacgaagata gaacctacac agcaatacga gaaatgtgta atttggtgct 240

tagcggtatt tatttaagca catgttggtg ttatagggca cttggattca gaagtttgct 300

gttaatttag gcacaggctt catactacat gggtcaatag tatagggatt catattatag 360

gcgatactat aataatttgt tcgtctgcag agcttattat ttgccaaaat tagatattcc 420

tattctgttt ttgtttgtgt gctgttaaat tgttaacgcc tgaaggaata aatataaatg 480

acgaaatttt gatgtttatc tctgctcctt tattgtgacc ataagtcaag atcagatgca 540

cttgttttaa atattgttgt ctgaagaaat aagtactgac agtattttga tgcattgatc 600

660

ctcctgatgg tatctagtat ctaccaactg acactatatt gcttctcttt acatacgtat 720

cttgctcgat gccttctccc tagtgttgac cagtgttact cacatagtct ttgctcattt 780

cattgtaatg cagataccaa gcgg 804

<210> 166

<211> 623

<212> DNA

<213> Cauliflower mosaic virus

<400> 166

ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg 60

cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg 120

ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa 180

agatggaccc ccacccga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 240

aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg 300

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 360

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 420

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 480

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 540

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 600

ttcatttgga gaggacacgc tga 623

<210> 167

<211> 8

<212> DNA

<213> Cauliflower mosaic virus

<400> 167

acacgctg 8

<210> 168

<211> 1790

<212> DNA

<213> Coix lacryma-jobi

<400> 168

gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata 60

caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag 120

taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact 180

tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc 240

acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg 300

ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag 360

gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat 420

ttccttcccc tcccctgccg ttctcctcat cataaatagc caccccctcc tcgcctcttt 480

tccccaactc atctgttctt cgtctcacac agccagatcc caatccctct cctcgcgaac 540

ttcgtcgatc tcccttccct cgcctcgctt caaggtacgg cgatcatcct cccgctttcc 600

ctcctcctcc tctagatgta gtacggagta cttgccatca tgcatcatgc tacatcacgc 660

tcgtgcgagc tctgggtcct cgatctggga acggaactgt gggatgctgc tcgtgcgatt 720

tattattgggg gatctgggtt ctcgatctgg gaacggaact gtgggatgct gctcgtgcga 780

ttttattattg gggatctggg ttctcgatct gggaacggaa ctgtgggatg cttgtaggca 840

ggtcggagat gggtcggatc gttgcttagg gttcgatctg ctcgtggttt tcttttaatc 900

cctgatgcat gatttatcgg tcatcctatt agatggaacc agtagggtga ctctgatccg 960

1020

gaccagtttt gctgtttttg gtatggttgt gatgcccgtc caaatatgac taagcgagtg 1080

tagatcatt ttatgaacta actgctggtc ttattaaatc tagatctgca tacgttgatg 1140

tactacgttc atagttgata cagtatgtat gaactagttg ctggtcgtat taattttgga 1200

tctgcatgtg tggtagcata taatgttcat aatacaattg atacagtatg atgtatgaac 1260

1320

taccatgatg gattaatcta tacttctgat gtatatgctg cagttttctg ctgaggctgt 1380

agttttttcc agattaaaat acagcatgca tatttgctaa gctctgggcg tgtgaacgcc 1440

caccatggca ttgtccagta atagtaatga atttttttgt ttgcctgatg tgggagaaaa 1500

cacgcattgt ccagttattt tgttccatat gcattgtcct gttttgttgg atatgcatgc 1560

ttagaaaaca tatgcagcca ctgtttgata atgctttagc atctgcctgt tgaacatgca 1620

1680

1740

ggtctactgc tatatgctta cccttttgtt tggctaattt tcaggtgcag 1790

<210> 169

<211> 481

<212> DNA

<213> Coix lacryma-jobi

<400> 169

gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata 60

caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag 120

taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact 180

tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc 240

acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg 300

ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag 360

gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat 420

ttccttcccc tcccctgccg ttctcctcat cataaatagc caccccctcc tcgcctcttt 480

t 481

<210> 170

<211> 93

<212> DNA

<213> Coix lacryma-jobi

<400> 170

ccccaactca tctgttcttc gtctcacaca gccagatccc aatccctctc ctcgcgaact 60

tcgtcgatct cccttccctc gcctcgcttc aag 93

<210> 171

<211> 1216

<212> DNA

<213> Coix lacryma-jobi

<400> 171

gtacggcgat catcctcccg ctttccctcc tcctcctcta gatgtagtac ggagtacttg 60

ccatcatgca tcatgctaca tcacgctcgt gcgagctctg ggtcctcgat ctgggaacgg 120

aactgtggga tgctgctcgt gcgatttatt attggggatc tgggttctcg atctgggaac 180

ggaactgtgg gatgctgctc gtgcgattta ttattgggga tctgggttct cgatctggga 240

acggaactgt gggatgcttg taggcaggtc ggagatgggt cggatcgttg cttagggttc 300

gatctgctcg tggttttctt ttaatccctg atgcatgatt tatcggtcat cctattagat 360

ggaaccagta gggtgactct gatccgatat acttaacctc gatctggttc gatgttcctg 420

gctaggcttg tgcgtctgtt tcgtcagacc agttttgctg tttttggtat ggttgtgatg 480

cccgtccaaa tatgactaag cgagtgtaga atcattttat gaactaactg ctggtcttat 540

taaatctaga tctgcatacg ttgatgtact acgttcatag ttgatacagt atgtatgaac 600

tagttgctgg tcgtattaat tttggatctg catgtgtggt agcatataat gttcataata 660

caattgatac agtatgatgt atgaactatc tgctggttta ttaaatttgg atctgcttgt 720

ggtaaaaaat atgtttttta tatagttacc atgatggatt aatctatact tctgatgtat 780

atgctgcagt ttttctgctga ggctgtagtt ttttccagat taaaatacag catgcatatt 840

tgctaagctc tgggcgtgtg aacgcccacc atggcattgt ccagtaatag taatgaattt 900

ttttgtttgc ctgatgtggg agaaaacacg cattgtccag ttattttgtt ccatatgcat 960

tgtcctgttt tgttggatat gcatgcttag aaaacatatg cagccactgt ttgataatgc 1020

tttagcatct gcctgttgaa catgcatgat ctacctatct ttatttttgta tgtacttggg 1080

1140

1200

taatttttcag gtgcag 1216

<---

Claims (31)

1. A recombinant DNA molecule for initiating transcription of a heterologous transcribed DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 52, 53, 55 and 56, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 52, 53, 55 and 56; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule. 2. A DNA molecule according to claim 1, characterized in that the heterologous transcribed DNA molecule is a gene of agronomic interest. 3. A recombinant DNA molecule according to claim 2, characterized in that the gene of agronomic interest causes plant tolerance to herbicides. 4. A recombinant DNA molecule according to claim 2, characterized in that the gene of agronomic interest causes resistance to pests in plants. 5. A genetic construct for initiating transcription of a heterologous transcribed DNA molecule, comprising a recombinant DNA molecule according to claim 1, wherein said DNA molecule is operably linked to the 3'UTR. 6. A transgenic plant cell containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 52, 53, 55 and 56, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 52, 53, 55 and 56; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and wherein said plant cell has increased expression of said heterologous transcribed DNA molecule compared to a plant cell lacking said recombinant DNA molecule. 7. Transgenic plant cell according to claim 6, characterized in that said transgenic plant cell is a monocotyledonous plant cell. 8. A transgenic plant cell according to claim 6, characterized in that said transgenic plant cell is a dicotyledonous plant cell. 9. A transgenic plant containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 52, 53, 55 and 56, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 52, 53, 55 and 56; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and wherein said transgenic plant has increased expression of said heterologous transcribed DNA molecule compared to a plant lacking said recombinant DNA molecule. 10. Part of a transgenic plant containing a recombinant DNA molecule containing a DNA sequence selected from the group consisting of: a) DNA sequences with at least 95 percent full-length sequence identity to any of SEQ ID nos: 52, 53, 55 and 56, where the sequence has promoter activity; And b) a DNA sequence containing any of SEQ ID nos: 52, 53, 55 and 56; wherein said DNA sequence is operably linked to a heterologous transcribed DNA molecule, and where the specified part of the transgenic plant has an increased expression of the specified heterologous transcribed DNA molecule compared to the part of the plant that does not contain the specified recombinant DNA molecule. 11. A transgenic seed from a transgenic plant according to claim 9 for obtaining a plant, where this seed contains the recombinant DNA molecule according to claim 1, and where the specified plant has an increased expression of the specified heterologous transcribed DNA molecule compared to a plant that does not contain the specified recombinant DNA molecule . 12. A method for expressing a heterologous transcribed DNA molecule, comprising the following steps: obtaining a transgenic plant according to claim 9 and cultivating said plant, wherein the transcribed DNA molecule is expressed. 13. A method for producing a transgenic plant, comprising the following steps: a) transforming a plant cell with a recombinant DNA molecule according to claim 1 to obtain a transformed plant cell; And b) regenerating the transgenic plant from the transformed plant cell; wherein said transgenic plant has increased expression of said heterologous transcribed DNA molecule compared to a plant lacking said recombinant DNA molecule.

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