RU2792843C2 - Universal vaccine based on common tumor neoantigens for the prevention and treatment of cancer with microsatellite instability (msi) - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: method for selecting a pool of peptides resulting from a frameshift (CFSP) to obtain a universal pool of peptides for a cancer vaccine (CVP) for the prevention and treatment of patients with hereditary and sporadic tumors with microsatellite instability (MSI). The present invention also relates to a method for producing CVPs by selecting a subset of frameshift peptides (FSPs) from CFSPs and optionally modifying the amino acid (ad) sequence of the FSPs to produce modified FSPs (mFSPs). The present invention further relates to pools of nucleic acids encoding CVP FSP and/or mFSP in one or more vaccine vectors, which can also be used simultaneously.

EFFECT: such CVPs, nucleic acids and vectors are used to prevent or treat MSI forms of cancer.

20 cl, 11 dwg, 2 tbl, 6 ex

Description

The present invention relates to a method for selecting a collection of frameshift peptides (CFSPs) to obtain a universal collection of cancer vaccine (CVP) peptides for the prevention and treatment of patients with hereditary and sporadic tumors with microsatellite instability (MSI). The present invention also relates to a method for producing CVPs by selecting a subset of frameshift peptides (FSPs) from CFSPs and optionally modifying the amino acid (aa) sequence of the FSPs to produce modified FSPs (mFSPs). The present invention further relates to collections of nucleic acids encoding CVP FSP and/or mFSP in one or more vaccine vectors, which can also be used simultaneously. Such CVPs, nucleic acids and vectors are useful in the prevention or treatment of MSI forms of cancer.

Background of the Invention

In the field of cancer vaccine development, attention has long been focused on targeting tumor-associated and, later, tumor-specific antigens. The latter may arise in cancer cells due to oncogenic viral proteins or somatic mutations in coding genes leading to the formation of tumor neoantigens defined as such because they are absent in normal cells. Causing lower risks of self-tolerance and autoimmune reactions, tumor neoantigens vary greatly among a given patient's cancer cells and across populations, making it difficult to develop an effective universal cancer vaccine based on common tumor neoantigens.

However, there is a group of cancers that do not follow this general rule due to their underlying nature: microsatellite instability (MSI) tumors, often caused by mutations in mismatch repair (MMR) genes. A defective MMR system causes the accumulation of mutations in regions of repetitive nucleotide sequences called microsatellites. Mutations in microsatellites of coding genes can cause a translational frameshift, resulting in the formation of chimeric proteins whose C-terminus consists of a new, unfamiliar peptide, the so-called frameshift peptide (FSP). Unlike most cancers, mutations in MSI tumors occur predominantly in microsatellites composed of mononucleotide repeats (MNRs). Within coding regions, such mutations mainly involve a 1 nucleotide deletion and affect a limited number of genes, thus being common among patients (Kim, T.M., et al. (2013) Cell 155(4): 858-868).

Therefore, MSI-associated cancers provide a unique opportunity to develop a universal vaccine based on common tumor neoantigens. MSI tumors include both sporadic and hereditary forms of cancer caused by somatic and gametic mutations in the MMR genes, respectively. Lynch syndrome (LS) is a rare disease (ORPHA144) that falls into the second group. In particular, individuals with heterozygous gamete mutations in the MSH2 or MLH1 genes encoding components of the MMR pathway (~90% of LS gene carriers) are at higher risk of developing cancer. In particular, they are predisposed to developing colon or endometrial cancer in >50% of cases during their lifetime (Boland, C.R. and A. Goel (2010) Gastroenterology 138(6): p. 2073-2087 e3).

Briefly, the invention described herein relates to the identification of common FSP neoantigens among MSI tumors and to the development of a universal vaccine based on a subset of FSPs and modified FSPs (defined as CVP) derived from CFSP for the treatment and prevention of MSI cancers. in particular, for LS carriers. Such CVPs may provide, inter alia, the following benefits: (i) appropriate therapeutic and/or prophylactic immune responses for a wide variety of MSI cancers; (ii) a mass-produced vaccine that is ready to use and effective in a large cohort of patients because it encodes a large set of common FSPs; (iii) a particular CVP can be selected to be suitable for the treatment and prevention of any cancer with MSI, which is particularly useful in a prophylactic setting; (iv) no risk of autoimmune responses due to exclusion of potential autoepitopes.

According to the knowledge obtained by the authors of the present invention, the methods of the present invention are characterized by various previously unknown features, including:

(i) a range of security measures such as

a) selection of frameshift peptides based on a gene that, on average, is expressed at a level above a threshold in the selected tumor type,

b) selection of frameshift peptides that are absent or extremely rare in the tissue of healthy subjects,

c) exclusion of frameshift peptide segments that are identical to segments of 8 amino acids or more in normal human proteins, and

c) using a large number of cancer samples.

(ii) Inclusion of frameshift peptides shorter than the length of the minimum expected CD8+ T cell epitope (8-mer). This is achieved by adding up to 4 wild-type amino acids to produce a peptide of at least 8 amino acids.

(iii) Selection of an optimal collection of frameshift peptides to be included in the vaccine such that each individual cancer sample (cancer samples represent the population targeted by the vaccine) contains a fraction of the frameshift peptides the reading frames encoded by the vaccine and the total length of the frameshift peptides present in this part was at least 400 amino acids. -cell response in each patient requires an average of 400 amino acids of the viral antigen, i.e. non-native antigens, such as frameshift peptides. Although for each cancer sample, the proportion of frameshift peptides covered will be different (i.e., each cancer sample harbors a different subset of frameshift mutations and therefore a different subset of frameshift peptides will be present from the optimal collection). frameshift), the optimal collection is selected such that for the proportion of frameshifted peptides covered in each tumor sample, the total length will be at least 400 amino acids. The incorporation of this 400 amino acid rule into the disclosed selection method thus provides for the induction of T cells against FSP present in tumors of the target population receiving the vaccine.

Brief summary of the present invention

According to a first aspect, the present invention relates to a method for selecting a collection of frameshift peptides (CFSP) to obtain a universal collection of peptides for a vaccine (CVP) against microsatellite instability (MSI) for the prevention or treatment of a patient with cancer, including hereditary and sporadic cancer with MSI, or at risk of developing such cancer, involving stages:

(i) selecting a collection of nucleic acids (CFSMs) each containing a frameshift mutation (FSM), each FSM present in one or more of at least M cancer samples (CS), each from a different patient, wherein the patient's cancer involves cancer cells with an MSI phenotype;

wherein at least 50% of the selected FSMs meet criteria (a), (b), (c) and/or (d):

(a) FSM is present in a mononucleotide repeat (MNR) of coding genes with a length of 6 nucleotides or more;

(b) FSM corresponds to a 1 nucleotide deletion;

(c) the number of FSM-carrying DNA sequencing reads is significantly higher in the tumor sample than in a comparable normal sample (FDR-corrected Fisher p-value equal to or less than 0.05);

(d) FSM is present in comparable normal samples with an allele frequency of less than 25%,

(ii) selecting X different frameshift peptides (FSPs), each selected FSP being a complete translation product of a nucleic acid protein-coding segment providing an FSM of the CFSM, at least 4 amino acids long, starting at the codon coding for the first amino acid that differs from the translation product of the corresponding wild-type (wt) nucleic acid without FSM,

wherein X is at least 20, more preferably at least 35, and M is at least 5.

According to a second aspect, the present invention relates to a method for determining the amino acid sequences of peptides contained in a CVP, or nucleic acid sequences encoding peptides contained in a CVP, comprising the steps of:

(a) selecting at least Y FSPs or fragments thereof at least 8 amino acids long from a CFSP selected in accordance with the first aspect of the present invention;

(b) modifying the amino acid sequence of one or more or all of those FSPs that meet the following criteria: (i) the FSP is 4 to 9 amino acids in length, and/or (ii) the FSP contains one or more identical contiguous stretches of 8 or more amino acids present in more than one FSP encoded by a nucleic acid with the same FSM, and/or (iii) the FSP contains one or more contiguous stretches of 8 or more amino acids also present in human wt proteins,

wherein the amino acid sequence of the FSP according to (i) is modified by adding to the N-terminus of the FSP from 1 to 4 amino acids of the wild-type (wt) amino acid sequence present immediately upstream of the FSP, and wherein the modified FSP (mFSP) is at least 8 amino acids in length ; the amino acid sequence of the FSP according to (ii) is modified by deleting such contiguous stretches from all but the longest FSP, provided that an FSP less than 4 amino acids long is excluded from the CVP after the removal of the contiguous stretch; and/or the amino acid sequence of the FSP according to (iii) is modified by removing such segments; modified FSPs less than 4 amino acids long are excluded from CVP after removal of the contiguous length, and

wherein the amino acid sequences of the CVPs include the amino acid sequences of the FSPs or fragments thereof selected in step a) and/or modified in step (b);

and wherein Y is at least 20, more preferably at least 35.

According to a third aspect, the present invention relates to a method for obtaining a CVP or a collection of nucleic acids encoding a CVP, comprising the steps

(i) obtaining information about amino acid sequences or nucleic acid sequences determined in the method according to the second aspect of the present invention; And

(ii) synthesizing the CVP amino acid sequence as one or more polypeptides or a collection of nucleic acids encoding such a sequence, and optionally inserting the collection of nucleic acids into one or more expression cassettes and/or a collection of expression vectors.

According to a fourth aspect, the present invention relates to a CVP or a collection of nucleic acids encoding peptides of said CVP, which can be obtained using the method according to the third aspect of the present invention.

According to a fifth aspect, a CVP comprising or consisting of Y different FSPs and/or mFSPs, wherein each FSP or FSP modified to form an mFSP is a complete translation product of a protein-coding segment of an FSM-containing nucleic acid, or a fragment thereof, starting with a codon encoding the first amino acid that differs from the translation product of the corresponding wt nucleic acid without FSM, in any case at least 4 amino acids long, and at least 50% of the FSP or FSP modified to form mFSP correspond to one or more from the following criteria:

(a) FSP is encoded by a nucleic acid with an FSM that is observed at a cancer type-specific frequency (CF) observed for a subset of CS of a particular cancer type that is part of a collection of M different CSs that is at least 5% of at least for one of the cancer types present in CS; and/or

(b) the average level of mRNA expression of the gene with FSM encoding FSP is in the upper 80th percentile of the distribution describing the average values of mRNA expression of each protein-coding gene by CS; and/or

(c) FSM causing FSP formation is observed in less than 2% of normal tissues in a cohort of subjects without cancer,

wherein the amino acid sequence of one or more or all of such FSPs that meet the following criteria: (i) the FSP is 4 to 9 amino acids in length, and/or (ii) the FSP contains one or more identical contiguous stretches of 8 or more amino acids present in more than one FSP encoded by a nucleic acid with the same FSM, and/or (iii) the FSP contains one or more contiguous stretches of 8 or more amino acids also present in human wt proteins,

modified for FSP according to (i) by adding to the N-terminus of FSP from 1 to 4 amino acids of the wild-type amino acid sequence (wt) present immediately upstream of the FSP, and wherein the modified FSP (mFSP) is at least 8 amino acids in length; for FSP in accordance with (ii) modified by removing such continuous segments from all but the longest FSP, provided that the FSP is less than 4 amino acids after the removal of the continuous segment is excluded from the CVP; and/or for FSP in accordance with (iii) modified by removing such segments; modified FSPs less than 4 amino acids long are excluded from CVP after removal of the contiguous segment;

and wherein Y is at least 20 and more preferably at least 35 and M is at least 5.

According to the sixth aspect, the present invention relates to a collection of nucleic acids encoding CVP according to the fifth aspect of the present invention.

According to the seventh aspect, the present invention relates to a collection of one or more expression vectors, each of which contains the entire collection of nucleic acids according to the fourth or sixth aspect of the present invention, or part thereof, and the complete collection of expression vectors contains the entire collection of nucleic acids according to the fourth or sixth aspect of the present inventions.

According to an eighth aspect, the present invention relates to a CVP according to the fourth or sixth aspect of the present invention, a collection of nucleic acids according to the fourth or sixth aspect of the present invention, a collection of expression vectors according to the seventh aspect of the present invention, for use in the prevention or treatment of a patient with cancer, involving cancer cells with MSI phenotype, or at risk of developing such a cancer, the cancer being preferably selected from the group consisting of colon and rectal cancer, gastric cancer, endometrial cancer, small intestine cancer, hepatobiliary tract cancer, liver cancer, neuroendocrine cancers, cervical cancer , ovarian cancer, forms of uterine sarcoma, brain cancer and skin cancer.

According to the eighth aspect, the present invention relates to a collection of nucleic acids according to the fourth or sixth aspect of the present invention and/or a collection of expression vectors according to the seventh aspect of the present invention for use in the prevention or treatment of a patient with cancer, involving cancer cells with an MSI phenotype, or at risk of developing such a cancer, wherein the collection of nucleic acids and/or the collection of expression vectors are administered using a heterologous prime-boost vaccination schedule, preferably priming with an adenovirus vector and one or more stimulations with an MVA vector.

Symbols on the figures

Fig. 1. The length of the antigen determines the amount of immunogenic epitopes induced by the vaccine.

Fig. 2. Immunogenic coverage of Nous-209 in MSI cell lines.

Fig. 3. Immunogenic coverage of Nous-209 in CRC biopsies with MSI.

Fig. 4. Number of predicted Nous-209-derived MHC-I-binding epitopes in biopsies from patients with MSI.

Fig. 5. FSP corresponding to SEQ ID NO: 123 included in Nous-209 is immunogenic in vivo in HLA.A02 transgenic mice. Representative response of CD8 T cells in 5 animals to the HLA-A02 nonamer present in FSP SEQ ID NO: 123 as measured by intracellular staining (ICS) for interferon (IFN-γ) (panel A). Graph of FACS results using a gating strategy for IFN-γ+CD8 T cells from one of these mice, showing a significant percentage (5.6%) of FSP-reactive T cells (panel B).

Fig. 6. Disorder profiles predicted by IUPRED for eight artificial polypeptides containing Nous-209 FSP in arrangement A and B.

Fig. 7. Schematic representation of the expression cassette.

Fig. 8. Immunogenicity of GAd20-209-FSP/MVA-209-FSP in priming/stimulation mode in mice. IFNγ ELISpot responses were measured 2 weeks after priming with GAd20-209-FSP (GAd) and one week after stimulation with MVA-209-FSP (GAd/MVA). Responses (number of IFN-γ producing T cells per million splenocytes) to 16 pools of synthetic peptides (P1-P16) spanning the vaccine-encoded 209 FSP polypeptide sequences are shown.

Fig. 9. Measurement of immune responses to each of 16 pools spanning 209 FSP sequences. IFNγ ELISpot responses were measured post-stimulation against splenocytes from mice vaccinated with GAd20-209-FSP/MVA-209-FSP. Shown are the responses (number of IFN-y producing T cells per million splenocytes) for each of the 16 pools of synthetic peptides (P1 to P16) spanning the vaccine-encoded 209 FSP polypeptide sequences.

Fig. 10. Vaccine-induced FSP immune responses encoded by a single vector are not affected by co-administration of the vectors. IFNγ ELISpot responses were measured post-priming (week 2, gray bars) and post-stimulation (week 3, black bars) in mice immunized with vector mixture or single vectors. Shown are the responses (number of IFN-γ producing T cells per million splenocytes) to 4 pools of synthetic peptides (P1-P4) spanning the FSP sequences encoded by the FSPA1 vector (A) and to 4 pools of synthetic peptides (P5-P8) spanning FSP sequences encoded by the FSPA2 vector (B). Statistical data were calculated using the non-parametric Mann-Whitney U-test (ns=p>0.05).

Fig. 11. FSP corresponding to SEQ ID NO: 123 (FSP peptide) included in Nous-209 is immunogenic in vivo in HLA.A02 transgenic mice. A) Percentage of IFNγ+ CD8+ FSP-specific T cell responses and responses observed with DMSP as a control. B) Plot of FACS results using a gating strategy for IFN-γ+ CD8 T cells from one representative mouse sample with DMSO as control (top) and FSP peptide (bottom).

Detailed disclosure of the present invention

Before the present invention is described in detail below, it should be understood that the present invention is not limited to the specific techniques, protocols, and reagents described herein, as they may vary. It should also be understood that the terminology used herein is only intended to describe specific embodiments, and is not intended to limit the scope of the present invention, which will be limited only by the appended claims. Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as are commonly understood by one of ordinary skill in the art.

Terms preferably used herein are defined as described in "A multilingual glossary of biotechnological terms: (IUPAC Recommendations)", Leuenberger, H.G.W, Nagel, B. and Klbl, H. eds. (1995), Helvetica Chimica Acta, CH-4010 Basel, Switzerland) and as described in "Pharmaceutical Substances: Syntheses, Patents, Applications" Axel Kleemann and Jurgen Engel, Thieme Medical Publishing, 1999; "Merck hidex: An Encyclopedia of Chemicals, Drugs, and Biologicals", edited by Susan Budavari et al., CRC Press, 1996, and United States Pharmacopoeia-25/National Formulary-20, published by United States Pharmcopeial Convention, Inc., Rockville Md., 2001.

In the present description and the following claims, unless the context otherwise requires, the word "comprise" and variations such as "comprises" and "comprising" will be intended to include the claimed feature, integer or step or group of features, integers or steps, but not the exclusion of any other feature, integer or stage or group of integers or stages. In the following paragraphs, various aspects of the present invention are defined in more detail. Each aspect thus defined may be combined with any other aspect or aspects, unless expressly stated otherwise. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.

Several documents are cited throughout the text of this specification. Each of the documents referenced in this document (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions, etc.), whether cited above or below, are incorporated herein by reference throughout its fullness. Nothing herein should be construed as an admission that the present invention is not entitled to claim primacy in filing the present disclosure by virtue of a prior invention.

Definitions

Below are some definitions of terms frequently used in the present description. Such terms, in each case of their use, in the rest of the description will have a correspondingly defined meaning and preferred meanings.

As used herein, the term "isolated" refers to a molecule that is substantially free of other molecules with which it is naturally associated. In particular, the term "isolated" means that the molecule is not found in an animal body or a sample from an animal body. An isolated molecule is thus free of other molecules with which it will encounter or come into contact in the animal body. "Isolated" does not mean separated from other associated components described herein, for example, not separated from other components of the composition in which the molecule is contained, or not isolated from the vector or cell in which it is contained.

The terms "polynucleotide" and "nucleic acid" are used interchangeably herein and are understood as a polymeric or oligomeric macromolecule consisting of monomers in the form of nucleotides. Nucleotide monomers are composed of a nitrogenous base, a five-carbon sugar (such as, but not limited to, ribose or 2'-deoxyribose), and one to three phosphate groups. Typically, a nucleic acid is formed via phosphodiester bonds between individual nucleotide monomers. In the context of the present invention, preferred nucleic acid molecules include, without limitation, ribonucleic acid (RNA), modified RNA, deoxyribonucleic acid (DNA), and mixtures thereof, such as, for example, RNA-DNA hybrids. Nucleic acids, for example, can be chemically synthesized, for example, according to the phosphotriester method (see, for example, Uhlmann, E. & Peyman, A. (1990) Chemical Reviews, 90, 543-584). "

The term "open reading frame", abbreviated as "ORF", in the context of the present invention is used to denote a sequence of nucleotides that can be translated into a consecutive chain of amino acids. Typically, an ORF contains a start codon, a subsequent region, usually a multiple of 3 nucleotides in length, but does not contain a stop codon (TAG, TAA, TGA, UAG, UAA, or UGA) in that reading frame. An ORF encodes a protein where the amino acids it can be translated into form a chain through peptide bonds.

In the context of this document, the terms "protein", "peptide", "polypeptide", "peptides" and "polypeptides" are used interchangeably throughout the text. These terms are used in the context of the present invention to refer to both naturally occurring peptides, such as naturally occurring proteins, and synthesized peptides, which may or may not include naturally occurring amino acids. Peptides can also be chemically modified by modifying the side chain or free amino or carboxy terminus of a naturally occurring or non-naturally occurring amino acid. Such chemical modification includes the addition of additional chemical moieties as well as modification of functional groups on the amino acid side chains, such as glycosylation. The peptide is a polymer, preferably containing at least 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or at least 100 amino acids, most preferably at least 8 or at least 30 amino acids.

The term "frameshift mutation", abbreviated "FSM", in the context of the present invention is used to refer to a change in the nucleic acid sequence within the ORF encoding a protein, which leads to a change in the reading frame below the mutation, and thus to the formation of a protein with an altered sequence compared to the wild-type protein. A frameshift mutation occurs when there is an insertion or deletion of a number of nucleotides in the ORF that is not a multiple of 3. Typically, FSM occurs when one or two nucleotides are deleted or inserted. Deletions are more common than insertions. If one or two nucleotides are removed within the ORF codon, an altered codon is generated with one or two nucleotides on the 3' side of the affected codon. If such a changed codon is not a stop codon, the ORF translation product will be determined by the changed codon and the alternate ORF on the 3' side of the changed codon until the next stop codon is reached. If there is an insertion of one or two nucleotides in a codon, a new codon is formed that will contain two or one nucleotide from the previous nucleotides and one or two of the inserted nucleotides. If such a new codon is not a stop codon, the ORF translation product will be determined by the new codon and the alternative ORF on the 3' side of the new codon until the next stop codon is reached.

The term "frameshift codon", abbreviated as "FC", is used in the context of the present invention to refer to the first codon on the 3' side of the FSM that codes for a different amino acid compared to the wt sequence.

The term "collection of nucleic acids providing FSM", abbreviated "CFSM", in the context of the present invention is used to refer to a list of individual nucleotide sequences, each of which includes FSM, which may represent all FSM observed in a particular type of cancer with MSI, for example, cancer colon, rectum, endometrium, or stomach, or two or more cancers with MSI, or a subgroup of FSM selected according to one or more of the criteria detailed below.

The term "frameshift peptide", abbreviated "FSP", in the context of the present invention is used to refer to the complete translation product of a protein-coding segment of a nucleic acid providing an FSM from CFSM, starting with FC.

The term “collection of frameshift peptides”, abbreviated as “CFSP”, is used in the context of the present invention to refer to a FSP amino acid sequence listing that may represent all FSPs seen in a particular type of cancer with MSI, e.g., colon cancer. and rectum, endometrium, or stomach, or two or more cancers with MSI, or a subset of FSP selected according to one or more of the criteria detailed below.

The term "modified FSP", abbreviated "mFSP", in the context of the present invention is used to refer to a peptide with an amino acid sequence based on FSP, but modified from FSP by addition or deletion of amino acids to make a particular FSP more suitable for inclusion in a collection of peptides. , which form a collection of peptides for a cancer vaccine. The criteria for selecting FSPs that may benefit from the modification and the specific modification are detailed below.

The term "collection of peptides for cancer vaccine", abbreviated as "CVP", in the context of the present invention is used to refer to FSP and/or mFSP, either in the form of separate peptides or associated with each other. Preferably, two or more different FSPs and/or mFSPs are linked to each other via peptide bonds to form polypeptides. The linkage may be direct or mediated by one or more linker amino acids, for example, small flexible amino acids such as Gly, Ser or Ala. In order to avoid the formation of additional antigens, it is preferable that the peptides are directly linked to each other. It is preferable to link FSP and/or mFSP to each other to form polypeptides. It is known that the efficiency of translation of very long mRNAs is reduced, and therefore it is preferable to separate CVPs containing FSPs and mFSPs with a total length of more than 1000 amino acids, more preferably more than 1500 amino acids, into separate polypeptides. For example, if the CVP contains FSP and/or mFSP with a total length of approximately 6000 amino acids, then it is preferred that the FSP and/or mFSP be linked to form four separate polypeptides, each containing an FSP and/or mFSP with a total length of approximately 1500 amino acids. .

The term "microsatellite instability", abbreviated as "MSI", is defined as changes in the length of microsatellites due to the deletion or insertion of one or more repeat units in microsatellite regions. This leads to the formation of new alleles of microsatellite loci with a change in the total length of the genomic DNA of tumor cells when compared with the genomic DNA of normal/germ cells from the same individual. The state of genetic hypermutability leads to disruption of mismatched nucleotide repair (MMR). MMR is a mechanism that corrects spontaneous mutations that occur in genomic DNA during DNA replication. Typically, mutations are single base pair mismatches or short nucleotide insertions or deletions. The last two can cause frameshift mutations if they occur in ORF and if the length of the inserted or deleted nucleotide sequence is not a multiple of three (see FSM definition above).

The term "MSI phenotype" refers to a change in the length of repetitive nucleotides, most commonly GT/CA repeats. Such repeats are found throughout the genomic DNA of healthy subjects, and make up approximately 3% of the human genome. One of skill in the art is well aware of how to determine the MSI phenotype in a sample. The preferred route is to use the 7 marker MSI (v 1.2) assay system from Promega (Madison, Wisconsin) in accordance with the guidelines established by the National Cancer Institute (NCI) Symposium on Microsatellite Instability.

The term "neoepitope" in the context of the present invention is used to refer to an epitope encoded by tumor genes that is absent in normal/germ cells, but occurs in precancerous and/or cancer cells, in particular those characterized by an MSI phenotype.

The term "expression cassette" in the context of the present invention is used to refer to a nucleic acid molecule that contains at least one nucleic acid sequence to be expressed, for example, a nucleic acid encoding a CVP of the present invention or a portion thereof operably linked to transcription and translation control sequences . Preferably, the expression cassette includes cis-regulatory elements for efficient expression of the gene, such as a promoter, an initiation site and/or a polyadenylation site. Preferably, the expression cassette contains all the additional elements required for the expression of the nucleic acid in the patient's cell. A typical expression cassette thus contains a promoter operably linked to the nucleic acid sequence to be expressed and the signals required for efficient polyadenylation of the transcript, ribosome binding sites and a transcription termination sequence. Additional elements of the cassette may include, for example, enhancers. The expression cassette preferably also contains a transcription termination region downstream of the structural gene to ensure efficient termination. The termination region may be derived from the same gene as the promoter sequence or may be derived from a different gene.

The term "operably linked" as used in the context of the present invention refers to an arrangement of elements in which the components thus described are configured to perform their normal function. A nucleic acid is "operably linked" if it is in a functional relationship with another nucleic acid sequence. For example, a promoter is operably linked to one or more transgenes if it affects the transcription of one or more transgenes. In addition, control elements operably linked to a coding sequence are capable of influencing the expression of the coding sequence. Control elements do not have to be adjacent to a coding sequence if they serve the function of directing its expression. Thus, for example, intermediate untranslated but transcribed sequences may be present between a promoter sequence and a coding sequence, and the promoter sequence is still considered to be "operably linked" to the coding sequence.

The term "expression vector" refers to a polynucleotide or a mixture of a polynucleotide and proteins that can be introduced, or that is capable of introducing a collection of nucleic acids of the present invention, or one nucleic acid that is part of a collection of nucleic acids of the present invention, into a cell, preferably a mammalian cell. Examples of vectors include, without limitation, plasmids, cosmids, phages, viruses, or artificial chromosomes. In particular, the vector is used to transfer a promoter and a collection of nucleic acids or a single nucleic acid that is part of the collection of nucleic acids of the present invention into a suitable host cell. Expression vectors may contain "replicon" polynucleotide sequences that facilitate autonomous replication of the expression vector in a host cell. Once in a host cell, the expression vector can replicate independently of, or simultaneously with, the host's chromosomal DNA, and multiple copies of the vector and the inserted DNA can be made. In the case of using replication-competent expression vectors - which is often the case for safety reasons - the vector may not replicate but simply direct the expression of the nucleic acid. Depending on the type of expression vector, the expression vector may be lost during cell division, i. it only transiently expresses the CVP encoded by the nucleic acid, or may be stably present in the cell. Expression vectors typically contain expression cassettes, ie. necessary elements that enable the transcription of a nucleic acid into an mRNA molecule.

The term "antigen" in the context of the present invention is used to refer to any structure recognized by immune response molecules, for example, antibodies, T-cell receptors (TCR), etc. Preferred antigens are cellular proteins that are associated with a particular disease. Antigens are recognized by the highly variable antigen receptors (B-cell receptor or T-cell receptor) of the adaptive immune system, and may elicit a humoral or cellular immune response. Antigens that elicit such a response are also called immunogens. A proportion of proteins within cells, whether foreign or cellular, are processed into smaller peptides and presented by the major histocompatibility complex (MHC). A cellular immune response is elicited when a small peptide fragment binds to a T-cell receptor.

The term "epitope", also known as an antigenic determinant, is used in the context of the present invention to refer to a segment of an antigen, preferably a peptide, that is bound by immune system molecules such as B-cell receptors, T-cell receptors or antibodies. Epitopes bound by antibodies or B cells are referred to as "B cell epitopes", and epitopes bound by T cells are referred to as "T cell epitopes". In this context, the term "binding" preferably refers to specific binding, which is defined as the binding of an antibody or T cell receptor (TCR) and the corresponding epitope with an association constant of 1x10 ^∧ 5 M-1 or higher, preferably 1x10 ^∧ 6 M -1, 1×10 ^∧ 7M-1, 1×10 ^∧ 8 M-1 or higher. One skilled in the art is well aware of how to determine the association constant (see, for example, Caoili, SE (2012) Advances in Bioinformatics Vol. 2012). Preferably, the specific binding of antibodies to the epitope is mediated by the Pab-(antigen-binding fragment-region of the antibody), the specific binding of the B cell is mediated by the Fab region of the antibody in the B-cell receptor, and the specific binding of the T-cell is mediated by the variable (V) region of the T-cell receptor. T -cell epitopes are presented on the surface of an antigen-presenting cell where they bind to major histocompatibility complex (MHC) molecules.There are at least three different classes of MHC molecules, referred to as MHC class I, II, and III, respectively.Epitopes presented by MHC-I- pathways elicit a response via cytotoxic T lymphocytes (CD8+ cells), while epitopes presented via the MHC-II pathway elicit a response via T helper cells (CD4+ cells). , are peptides 8-11 amino acids long, and T-cell epitopes presented by mo Class II MHC molecules are typically 13-17 amino acid long peptides. Class III MHC molecules also present non-peptide epitopes such as glycolipids. Accordingly, the term "T-cell epitope" preferably refers to peptides 8-11 or 13-17 amino acids long, which can be represented by either MHC class I or MHC class II molecules. Epitopes typically consist of reactive groups on the surface of amino acids that may or may not carry side chains from sugars, and typically have specific three-dimensional structure characteristics as well as specific charge characteristics. Conformational and non-conformational epitopes differ in that binding to the former, but not to the latter, is lost in the presence of denaturing solvents.

The term "linked epitope" as used in the context of the present invention refers to an epitope that is not present in the isolated FSP, mFSP and/or antigenic fragment thereof of the given CVP, but contains the amino acid sequence resulting from the linking of two peptides, e.g. two FSPs, via a peptide connections. For example, a list of all possible contiguous epitopes of the assembled peptides with 8 consecutive amino acids can be created, which includes 1 to 7 amino acids of the first peptide and 7 to 1 amino acids of the second peptide, including the binding peptide bond. This list is then compared with the amino acid sequence of all FSPs and mFSP CVPs in order to identify all possible linked epitopes.

The term "epitope found in non-MSI cancer" refers to a protein epitope that is specifically expressed in cancer cells but is not associated with FSM. Such an epitope is considered specific if its amount in cancer cells is at least 10 times greater than in healthy cells. Examples of such epitopes are proteins that are overexpressed in cancer cells, such as tyrosinase in melanoma forms or the Her-2 receptor in breast cancer, or which are mutated in certain cancers, such as p53.

The term "immunogenic coverage" refers to the expected number of immunogenic epitopes that a vaccine can stimulate in a patient. A vaccine encoding a set of FSPs with a total length of 400 amino acids that are present in a patient's tumor is expected to stimulate an average of 3 immunogenic epitopes and provide good immunogenic coverage.

The term "antigenic fragment thereof" as used in the context of the present invention refers to an antigen fragment, preferably FSP or mFSP, where the fragment is also antigenic, i.e. capable of eliciting a B- and/or T-cell immune response in a mammal. Preferably, the antigenic "FSP and/or mFSP fragments" are continuous FSP and mFSP segments of at least

8 amino acids as defined above and described in more detail below. The mFSP fragments contain at least 4 amino acids of the FSP from which the mFSP is derived.

The term "collection of nucleic acids encoding CVP" in the context of the present invention is used to refer to one or more consecutive nucleic acid sequences encoding a polypeptide containing all FSP and/or mFSP CVP, or antigenic fragments thereof, encoding 2, 3, 4 , 5, 6, 7, 8, 9 or 10 or more, preferably four polypeptides containing all of the FSP and/or mFSP CVP. Thus, in one embodiment, the term includes a single CVP-encoding nucleic acid and, in another extreme embodiment, the collection of nucleic acids includes a single nucleic acid for each FSP and/or mFSP or antigenic fragment thereof.

The terms "preparation" and "composition" as used in the context of the present invention are intended to include a formulation based on the active compound, eg the VLP of the present invention, with a carrier and/or an excipient.

The term "pharmaceutically acceptable" as used in the context of the present invention means approved by a federal or state government regulatory agency or listed in the USP or other generally accepted pharmacopeia for use in animals, and in particular in humans.

The term "carrier" in the context of this document refers to a pharmacologically inactive substance, such as, without limitation, a diluent, excipient, surfactants, stabilizers, physiological buffer solutions, or inert substances, with which the therapeutically active ingredient is administered. Such pharmaceutical carriers may be liquid or solid. Liquid carriers include, without limitation, sterile liquids such as saline solutions in water and oils, including but not limited to those of petroleum, animal, vegetable, or synthetic origin such as peanut oil, soybean oil, mineral oil, sesame oil, and the like. Saline solutions and aqueous solutions of dextrose and glycerol can also be used as liquid carriers, in particular for injectable solutions. When the pharmaceutical composition is administered intravenously, saline is the preferred carrier. Examples of suitable pharmaceutical carriers are described in "Remington's Pharmaceutical Sciences" by E.W. Martin.

Suitable pharmaceutical "excipients" include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, skimmed milk powder, glycerin, propylene, glycol, water, ethanol, etc.

"Surfactants" includes anionic, cationic, and nonionic surfactants such as, but not limited to, sodium deoxycholate, sodium dodecyl sulfate, Triton X-100, and polysorbates such as polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 65, and polysorbate 80 .

"Stabilizers" include, without limitation, mannitol, sucrose, trehalose, albumin, and protease and/or nuclease antagonists.

"Physiological buffer solution" that can be used in the context of the present invention includes, without limitation, sodium chloride solution, demineralized water, as well as suitable organic or inorganic buffer solutions, such as, without limitation, phosphate buffer, nitrate buffer, tris buffer (tris(hydroxymethyl )aminomethane), HEPES buffer ([4-(2-hydroxyethyl)piperazino]ethanesulfonic acid) or MOPS buffer (3-morpholino-1-propanesulfonic acid). The selection of an appropriate buffer generally depends on the molarity of the buffer. Phosphate buffer is suitable, for example, for injection and infusion solutions.

The term "adjuvant" refers to agents that enhance, stimulate, activate, potentiate, or modulate the immune response to the active ingredient of the composition, either at the cellular or humoral level, for example, immunological adjuvants stimulate the response of the immune system to an actual antigen, but do not by themselves have an immunological effect. Examples of such adjuvants include, without limitation, inorganic adjuvants (for example, inorganic metal salts such as aluminum phosphate or aluminum hydroxide), organic adjuvants (for example, saponins or squalene), oil-based adjuvants (for example, complete Freund's adjuvant and incomplete Freund's adjuvant) , cytokines (eg, IL-1β, IL-2, IL-7, IL-12, IL-18, GM-CFS, and INF-γ), particulate adjuvants (eg, immunostimulatory complexes (ISCOMS), liposomes, or biodegradable microspheres), virosomes, bacterial adjuvants (eg, monophosphoryl lipid A or muramyl peptides), synthetic adjuvants (eg, nonionic block copolymers, muramyl peptide analogs, or synthetic lipid A), or synthetic polynucleotide adjuvants (eg, polyarginine or polylysine).

An "effective amount" or "therapeutically effective amount" is an amount of a therapeutic agent sufficient to achieve the intended purpose. The effective amount of a given therapeutic agent will vary depending on factors such as the nature of the agent, the route of administration, the size and species of the animal to be treated with the therapeutic agent, and the purpose of administration. The effective amount in each individual case can be determined empirically by a person skilled in the art in accordance with well-established methods in this field.

As used herein, "treating", "administering treatment", "treatment", or "treatment" of a disease or disorder means effecting one or more of the following: (a) lessening the severity of the disorder; (b) limiting or preventing the development of symptoms characteristic of the disorder(s) to be treated; (c) inhibiting worsening of symptoms characteristic of the disorder(s) to be treated; (d) limiting or preventing recurrence of the disorder(s) in an individual who has previously experienced the disorder(s); and (e) limiting or preventing recurrence of symptoms in individuals who have previously experienced symptoms of the disorder(s).

Aspects of the present invention and preferred embodiments

According to a first aspect, the present invention relates to a method for selecting a collection of frameshift peptides (CFSP) to obtain a universal collection of peptides for a vaccine (CVP) against microsatellite instability (MSI) for the prevention or treatment of a patient with cancer, including hereditary and sporadic cancer with MSI, or at risk of developing such cancer, involving stages:

(i) selecting a collection of nucleic acids (CFSMs) each containing a frameshift mutation (FSM), each FSM present in one or more of at least M cancer samples (CS), each from a different patient, wherein the patient's cancer involves cancer cells with an MSI phenotype;

wherein at least 50% of the selected FSMs meet criteria (a), (b), (c) and/or (d):

(a) the FSM is present in a mononucleotide repeat (MNR) of coding genes with a length of 6 nucleotides or more;

(b) FSM corresponds to a 1 nucleotide deletion;

(c) the number of FSM-carrying DNA sequencing reads is significantly higher in the tumor sample than in a comparable normal sample (FDR-corrected Fisher p-value equal to or less than 0.05);

(d) FSM is present in comparable normal samples with an allele frequency of less than 25%,

(ii) selecting X different frameshift peptides (FSPs), each selected FSP being a complete translation product of a nucleic acid protein-coding segment providing an FSM of the CFSM, at least 4 amino acids long, starting at the codon coding for the first amino acid that differs from the translation product of the corresponding wild-type (wt) nucleic acid without FSM,

wherein X is at least 20, more preferably at least 35, and M is at least 5.

By selecting a certain number and types of FSPs in accordance with the method according to the first aspect, the induction of T cells in relation to a certain number of FSPs is ensured by vaccination. The expected number of immunogenic epitopes for T cells based on the 400 aa rule is at least one immunogenic epitope for every 400 aa, and therefore at least 16 for a vaccine encoding a polypeptide with a total length of 6021 aa. The present inventors actually demonstrated in a mouse model that vaccination with 209 FSPs with a total length of 6021 aa induced a T cell response to at least 16 different FSPs, corresponding to at least 1 in every 376 aa (Fig. 9), confirming the rule immunogenicity. The chosen platform for vaccination based on genetic vectors, as opposed to peptide vaccination, ensured that the immunogenicity of an individual FSP was not suppressed by other FSPs present in the vaccine. The present inventors have demonstrated in a mouse model that competition does not occur by showing that administration of only one of the four vaccine vectors elicits nearly identical responses when compared to administration of a mixture of four vaccine vectors.

In one embodiment, the FSPs selected in step (ii) can be described as candidate FSPs for a generic MSI CVP for the prevention or treatment of a patient with or at risk of developing hereditary and sporadic cancer with MSI. The term "candidate" means that FSPs have a potential prophylactic or therapeutic effect. This can be expected based on the selection process and their structure.

According to the selection criterion (c), it is required that the occurrence of a given FSM be significantly higher, i.e. that it occurs more frequently in the genomic DNA of one or more tumor samples than in comparable normal samples from a healthy subject or healthy subjects. In this context, the term "significantly higher" means that the occurrence of a given FSM relative to the occurrence of unmutated MNR wt is significantly higher in a tumor sample compared to a normal sample according to the Fisher's p-value adjusted for the mean false rejection rate (FDR), equal to or less than 0.1, more preferably less than 0.05, more preferably less than 0.01, and even more preferably less than 0.005. The lower the value, the more suitable the FSM is for inclusion in the CFSM, since it is less likely to stimulate an unwanted immune response to healthy tissue. This criterion is assessed by sequencing either genomic DNA or cDNA obtained from the samples. Preferably, criterion (c) is met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least at least 95% and most preferably at least 100%.

While criterion (c) refers to the relative occurrence of a given FSM, criterion (d) refers to the absolute occurrence of a given FSM in alleles for normal samples. Preferably, the FSM selected for CFSM has a low overall occurrence in alleles for normal samples. Preferably the allele frequency in normal samples is less than 40%, more preferably less than 35%, more preferably less than 30%, more preferably less than 25%, more preferably less than 20% and even more preferably less than 10%. Preferably, criterion (d) is met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least at least 95% and most preferably at least 100%.

Preferably, the FSMs selected for CFSM meet both criteria (c) and (d). Preferably, criteria (c) and (d) are met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90% , more preferably at least 95% and most preferably at least 100%. Preferably at least 50% also meet criteria (a) and/or (b).

According to another preferred embodiment, the FSMs selected for the CFSM meet criterion (a). Preferably, criterion (a) is met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least at least 95% and most preferably at least 100%.

According to another preferred embodiment, the FSMs selected for the CFSM meet criterion (b). Preferably, criterion (b) is met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least at least 95% and most preferably at least 100%.

According to another preferred embodiment, the FSMs selected for the CFSM meet criteria (a), (c) and (d). Preferably, criteria (a), (c) and (d) are met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least 95% and most preferably at least 100%.

According to another preferred embodiment, the FSMs selected for CFSM meet criteria (b), (c) and (d). Preferably, criteria (b), (c) and (d) are met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least at least 90%, more preferably at least 95% and most preferably at least 100%.

According to another preferred embodiment, the FSMs selected for the CFSM meet criteria (a), (b), (c) and (d). Preferably, criteria (a), (b), (c) and (d) are met by 50% of the FSMs selected for CFSM, more preferably at least 60%, more preferably at least 70%, more preferably at least 80% , more preferably at least 90%, more preferably at least 95% and most preferably at least 100%.

The method of the present invention provides a CVP that is suitable for treating cancer with MSI, not only in a particular patient, but in many different patients who have cancer with MSI, ie. a versatile CVP for the treatment of cancers with MSI. For the same reason, it will also be suitable in the context of having a preventive effect on the development of cancer forms with MSI. Accordingly, the selection of FSM in the method of the present invention is not based on the analysis of a cancer sample from only one patient, but on the contrary, it uses information for several cancer samples from different patients. To obtain appropriate diversity, at least 5 cancerous tumor (CS) samples from different patients are analyzed to determine FSM. As described above, this analysis is preferably performed using whole genome sequencing, exome sequencing or cDNA sequencing obtained from mRNA isolated from samples. Use cancer samples from patients who have cancer with MSI. This can be determined by detecting the MSI phenotype in the cells contained in the cancer sample. CS may refer to the same cancer as MSI, ie. originate from the same tissue, or from two or more different cancers with MSI. This is particularly advantageous and provides a particularly widely used CVP if CS of MSI cancers of two or more different MSI cancers are used in the selection process. Preferred cancers with MSI from which samples originate are colon and rectal cancer and gastric cancer, colon and rectal cancer and endometrial cancer, gastric and endometrial cancer and gastric cancer, colon and rectal cancer and endometrial cancer.

The selection process described in terms of the FSM can also be performed based on the FSP encoded by the particular nucleic acid providing the FSM, ie. criteria (c) and (d) can also be assessed by comparing the encoded amino acid sequences, respectively, rather than nucleic acid sequences. It will immediately be apparent to one skilled in the art that the presence of FSM in a cancerous tumor or normal sample can also be assessed by determining the number of amino acid sequences of the encoded proteins containing FSPs at their C-terminus and not at the C-terminus of the wild-type protein sequence.

The present inventors also contemplate selecting FSPs that are included in the CFSP using one or more of the following criteria:

(a) FSP is encoded by a nucleic acid with an FSM that is observed at a cancer type-specific frequency (CF) observed for a subset of CS of a particular cancer type that is part of a collection of M different CSs that is at least 5% of at least for one of the cancer types present in CS; and/or

(b) the average level of mRNA expression of the gene with FSM encoding FSP is in the upper 80th percentile of the distribution describing the average values of mRNA expression of each protein-coding gene by CS; and/or

(c) FSM, causing the formation of FSP, is observed in less than 2% of normal tissues in a cohort of subjects without cancer.

Preferably, at least 50% of the FSPs included in the CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c), or (a )+(b)+(c). Preferably, 60% of FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or (a) +(b)+(c), more preferably at least 70% of the FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c ), (b)+(c) or (a)+(b)+(c), more preferably at least 80% of the FSPs selected for CFSP meet criteria (a), (b), (c), ( a)+(b), (a)+(c), (b)+(c) or (a)+(b)+(c), more preferably at least 90% of the FSPs selected for CFSP meet the criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or (a)+(b)+(c), more preferably that at least 95% of the FSPs selected for the CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or ( a)+(b)+(c), and most preferably at least 100% of the FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a) +(c), (b)+(c) or (a)+(b)+(c).

With respect to criterion (a), it is preferable that FSP be encoded by a nucleic acid with an FSM that is observed with a CF observed for a subset of CS of a specific cancer type that is part of a collection of M different CSs that is at least 5% for at least one or all of the cancer types present in CS, preferably at least 10%, preferably at least 15%, preferably at least 20%, preferably at least 25%, preferably at least 30%, more preferably at least 35%, more preferably at least 40% for at least one or all of the cancer types present in CS.

With regard to criterion (b), it is preferable that the average expression level of the mRNA of the FSM gene encoding FSP is in the upper 75th percentile, preferably the upper 70th percentile, preferably the upper 65th percentile, preferably the upper 60th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer. The higher the relative amount of FSP, the more likely that the induction of an immune response will be effective in the treatment or prevention of forms of cancer with MSI. Solely for clarity, the phrase "upper 80th percentile" refers to all FSMs in which the level of expression is in the range including mRNAs expressed at the highest level and excluding only 19% of mRNAs expressed at the lowest level. Accordingly, the "upper 30th percentile" refers to all FSMs in which the expression level includes the mRNAs expressed at the highest level and excludes 69% of the mRNAs expressed at the lowest level.

Preferred combinations of criteria (a) and (b) are a CF of at least 5% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the mean values of mRNA expression of each protein-coding gene by CS, preferably of a specific type cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 10% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 15% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 20% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer.

With regard to criterion (c), it is preferable that the FSM causing FSP formation is observed in less than 2% of normal tissues in a cohort of subjects without cancer, more preferably in less than 1.9%, less than 1.8%, less than in 1.7%, less than 1.6%, less than 1.5%, less than 1.4%, less than 1.3%, less than 1.2%, less than 1 .1%, less than 1.0% or less.

According to a preferred embodiment of the method according to the first aspect of the present invention:

(i) CS are obtained from patients who have a tumor with MSI, preferably colon and rectal cancer, and/or gastric cancer, and/or endometrial cancer, more preferably colon and rectal cancer, gastric cancer and endometrial cancer; and/or

(ii) M is at least 30, preferably at least 50, more preferably at least 100, more preferably at least 200, most preferably at least 300; and/or

(iii) X is at least 50, preferably at least 100, more preferably at least 200, and more preferably at least 300.

Preferably M is at least 30 and X is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, preferably M is at least 50 and X is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, M is at least 100 and X is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, M is at least 200 and X is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, even more preferably M is at least 300 and X is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least e 300.

An increase in the number of M samples determines a wider, i.e. more versatile, CVP applicability. However, due to the statistical distribution of FSMs, the completeness of detection of all FSMs occurring in a particular type of cancer with MSI will asymptotically reach a maximum for a given number of samples. Thus, when more than 400 samples of a particular type of cancer with MSI are included, no meaningful improvement will be achieved. To increase the broad coverage of FSMs present in an MSI type of cancer, it is preferable to use CS of two or more MSI cancers. Preferred cancers with MSI that are treatable and preventable at the same time are colon and rectal cancer and gastric cancer, colon and rectal cancer and endometrial cancer, gastric and endometrial cancer and colon and rectal cancer, gastric cancer and endometrial cancer.

According to a second aspect, the present invention relates to a method for determining the amino acid sequences of peptides contained in a CVP, or nucleic acid sequences encoding peptides contained in a CVP, comprising the steps of:

(a) selecting at least Y FSPs or antigenic fragments thereof from a CFSP selected in accordance with the first aspect of the present invention;

(b) modifying the amino acid sequence of one or more or all of those FSPs that meet the following criteria: (i) the FSP is 4 to 9 amino acids in length, and/or (ii) the FSP contains one or more identical contiguous stretches of 8 or more amino acids present in more than one FSP encoded by a nucleic acid with the same FSM, and/or (iii) the FSP contains one or more contiguous stretches of 8 or more amino acids also present in human wt proteins,

wherein the amino acid sequence of the FSP according to (i) is modified by adding to the N-terminus of the FSP 1, 2, 3 or 4, preferably 4 amino acids of the wild-type amino acid sequence (wt) present immediately upstream of the FSP, and wherein the modified FSP (mFSP) has at least 8 amino acids long; the amino acid sequence of the FSP according to (ii) is modified by deleting such contiguous stretches from all but the longest FSP, provided that an FSP less than 4 amino acids long is excluded from the CVP after the removal of the contiguous stretch; and/or the amino acid sequence of the FSP according to (iii) is modified by removing such segments; modified FSPs less than 4 amino acids long are excluded from CVP after removal of the contiguous length, and

moreover, the amino acid sequences of the CVP include the amino acid sequences of the FSPs or their antigenic fragments selected in step a) and/or modified in step (b);

and wherein Y is at least 20, more preferably at least 35.

In the context of the second aspect, it should be noted that the term "definition" is used, as is immediately obvious from step (b), not in the sense of "identifying" the sequence of peptides (for example, by sequencing), but in the sense of "establishing", "assigning" , "encryption" or "modification".

In one embodiment, at least one of the FSPs modified in step (b) is i) immunogenic, and ii) present in the patient's tumor, or expected to be so. Preferably, the immunogenicity of such a FSP is not suppressed by another selected or modified FSP.

In a further embodiment, the FSPs modified in step (b) are candidate FSPs for a generic MSI CVP for the prevention or treatment of a patient with or at risk of developing cancer, including hereditary and sporadic cancer with MSI.

The modification of the FSP according to step (b) serves to increase the antigenicity of the FSP. Preferably, at least 50% of FSPs that meet criteria (i), (ii), (iii), (i)+(ii), (i)+(iii), (ii)+(iii) or ( i)+(ii)+(iii) have been modified, more preferably at least 60% of FSPs that meet criteria (i), (ii), (iii), (i)+(ii), (i)+ (iii), (ii)+(iii) or (i)+(ii)+(iii) have been modified by at least 70% of FSPs that meet criteria (i), (ii), (iii), ( i)+(ii), (i)+(iii), (ii)+(iii) or (i)+(ii)+(iii) have been modified, more preferably at least 80% of FSPs that meet the criteria (i), (ii), (iii), (i)+(ii), (i)+(iii), (ii)+(iii) or (i)+(ii)+(iii) have been modified , more preferably at least 90% FSP that meet criteria (i), (ii), (iii), (i)+(ii), (i)+(iii), (ii)+(iii) or ( i)+(ii)+(iii) have been modified, more preferably at least 95% of FSPs that meet criteria (i), (ii), (iii), (i)+(ii), (i)+ (iii), (ii)+(iii) or (i)+(ii)+(iii) have been modified, and most preferably 100% FSP that meet criteria (i), (ii), (iii), (i)+(ii), (i)+(iii), (ii)+(iii) or (i)+( ii)+(iii) have been modified.

According to a preferred embodiment of the second aspect of the present invention, the selection of FSPs and mFSPs is carried out in step (a), in which FSPs are sequentially selected from CFSPs, and in each selection step, a new FSP is selected from CFSPs that increases the total total amino acid length of FSPs (CAFSPL) to reach the threshold in the maximum number of cancer samples whose CAFSPL is below that threshold, and optionally, if more than one FSP raises CAFSPL for the maximum number of cancer samples in which the CAFSPL is still below the threshold, select the FSP with the highest score .

According to a preferred embodiment of the second aspect of the present invention:

(i) CAFSPL for each cancer sample is determined by summing the amino acid length of the FSPs that are already part of the CVP and the new FSP from the CFSP for which the corresponding FSMs are present in the cancer sample; and/or

(ii) where the threshold value is determined separately for each subset of samples from the CS, which belong to a particular type of cancer; and/or

(iii) where the score is defined as the product of the length in amino acids of the FSP and the overall frequency of occurrence with which the FSM causing the formation of FSP is observed in CS, optionally without taking into account FSM in CS from a type of cancer for which CF is below 5%, more preferably below 4 %, more preferably below 3%, and/or

(iv) wherein the subset of cancer samples comprises all cancer samples from tumor types in which FSM is present with a CF greater than or equal to 5%, more preferably greater than or equal to 10%, greater than or equal to 15%; and/or

(v) moreover, FSPs resulting from FSM with an overall frequency of occurrence below 5%, more preferably below 4%, more preferably below 3%, are excluded from the selection; and/or

(vi) adding new FSPs and/or modified FSPs until there are no available FSPs whose inclusion in the CVP would increase the CAFSPL of any cancer sample whose CAFSPL is still below Threshold (TV), or the total length of all FSPs present in the CVP will not reach the maximum V amino acid value; and/or

(vii) wherein the FSPs arising from the same FSM are considered as one FSP with the cumulative score calculated as the sum of the scores for the individual FSPs.

Criteria (i)-(vii) serve to increase the versatility of the FSPs selected for CVP by providing high immunogenic coverage for a cohort of patients with one or more cancer types with MSI. In addition, criterion (vi) also allows the selection of CVP, which is practical by limiting the total number of antigens that can be encoded in the vaccine. The use of a scoring system makes it possible that FSPs with a higher observed frequency of occurrence (hence, they are expected to be present in a large number of patients) and with a greater total amino acid length (i.e., FSP with a higher total immunogenic potential).

In a preferred embodiment, at least 50%, more preferably at least 60%, at least 70%, at least 80%, at least 90%, meet criteria (i)+(iii)+(vi). at least 95% FSP.

Thus, it is preferred that at least 50% of the FSPs are selected, preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi) , (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more than preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii )+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+ (vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+( vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+ (vi)+(vii), more preferably at least 60% of the FSPs are selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+( iii)+(vi), (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi) +(vii), more preferably based on criteria (i )+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii)+ (vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or (i )+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), (i) +(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+( iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+(vii ), more preferably at least 70% of the FSPs are selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi) , (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more than preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii )+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+ (vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+( vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(i ii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+(vii ), more preferably at least 80% of the FSPs are selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi) , (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more than preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii )+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+ (vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+( vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+ (vi)+(vii), more preferably at least 90% of the FSPs are selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+( iii)+(vi), (i)+(iii)+(iv )+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria (i)+(ii) +(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii)+(vi)+( vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or (i)+(iii) +(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), (i)+(ii)+ (iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv )+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+(vii) and most preferably, that 100% FSPs be selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi), (i)+(iii) +(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria (i)+ (ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii)+(vi )+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or (i)+ (iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+( iv)+(v)+(vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v) +(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+ (iv)+(v)+(vi)+(vii).

It will be immediately apparent to one skilled in the art that the score can also be determined in alternative ways, for example, based only on the total frequency of occurrence of the FSM, which causes the formation of the FSP, the length or reciprocal of the FSP length, or as the sum of the cancer type-specific scores, or after excluding one or more types of cancer from the score calculation. The alternative score may further include or be based solely on the number of predicted epitopes presented by MHC class I and/or class II within each FSP. It will also be immediately apparent to one of ordinary skill in the art that criterion (vii) can be modified if the expression levels of specific mRNA isoforms conferring FSM are known. In such a case, one or more of the FSPs resulting from the same FSM may be excluded from selection, or the score of FSPs derived from individual FSM isoforms of mRNAs may be weighted according to observed relative isoform expression levels.

According to a preferred embodiment of the second aspect of the present invention:

(i) the addition of new FSPs continues until there are no available FSPs whose inclusion in the CVP would increase the CAFSPL of any cancer sample whose CAFSPL is still below TV; and/or

(ii) FSPs arising from the same FSM are treated as one FSP with the cumulative score calculated as the sum of the scores for the individual FSPs.

According to a preferred embodiment of the second aspect of the present invention:

(a) TV is at least 400 amino acids, preferably at least 600 amino acids, more preferably at least 800 amino acids; or

(b) TV is:

(i) at least 400 amino acids, preferably at least 600 amino acids, more preferably at least 800 amino acids for colon and rectal cancer and gastric cancer; And

(ii) at least 200 amino acids, preferably at least 300 amino acids, more preferably at least 400 amino acids for endometrial cancer.

One skilled in the art will immediately appreciate that TV values can be extended to any situation where selection is made for CS that includes different types of cancer, e.g. combinations of colon and rectal tumors, gastric tumors, endometrial tumors, small intestine tumors. , tumors of the hepatobiliary tract, liver tumors, neuroendocrine tumors, cervical tumors, ovarian tumors, forms of uterine sarcoma, brain cancer and / or skin tumors.

According to a preferred embodiment of the second aspect of the present invention:

(i) the CVP contains at least 4 amino acids of each FSP selected from the CFSP; and/or

(ii) Y is selected such that the total length in V amino acids of all peptides that are part of the CVP is at least 280 amino acids, preferably at least 6000;

(iii) Y is at least 35, preferably at least 50, preferably at least 100, more preferably at least 200, and/or

(iv) the CVP contains FSP and/or mFSP selected from the FSP and/or mFSP group according to SEQ ID NO: 1-1087, preferably selected from the FSP and/or mFSP group according to SEQ ID NO: 1-209.

According to a third aspect, the present invention relates to a method for obtaining a CVP or a collection of nucleic acids encoding a CVP, comprising the steps

(i) obtaining information about amino acid sequences or nucleic acid sequences determined in the method according to the second aspect of the present invention; And

(ii) synthesizing the CVP amino acid sequence as one or more polypeptides or a collection of nucleic acids encoding such a sequence, and optionally inserting the collection of nucleic acids into one or more expression cassettes and/or a collection of expression vectors.

Synthesis of FSP and, if modified, mFSP CVP, or antigenic fragments thereof, can be carried out by recombinant expression of a collection of nucleic acids encoding FSP, and, if modified, mFSP, or antigenic fragments thereof, or by chemical synthesis. Similarly, the synthesis of a collection of nucleic acids encoding CVP peptides can be carried out using known methods, including chemical solid phase synthesis.

According to a fourth aspect, the present invention relates to a CVP that can be obtained using the method according to the second aspect of the present invention, or a collection of nucleic acids encoding peptides of said CVP.

According to a fifth aspect, the CVP comprises or consists of Y different FSPs and/or mFSPs or FSP and/or mFSP antigenic fragments of at least 8 amino acids in length, wherein each FSP or FSP modified to form an mFSP is a complete protein translation product. a coding segment of an FSM-containing nucleic acid, starting at a codon encoding the first amino acid that differs from the translation product of the corresponding wt nucleic acid without FSM, in any case at least 4 amino acids long, and at least 50% FSP or FSP, modified to form mFSP meet one or more of the following criteria:

(a) FSP is encoded by a nucleic acid with an FSM that is observed with a cancer type-specific frequency (CF) observed for a subset of CS of a specific cancer type that is part of a collection of M different CSs that is at least 5% of at least for one of the cancer types present in CS; and/or

(b) the average level of mRNA expression of the gene with FSM encoding FSP is in the upper 80th percentile of the distribution describing the average values of mRNA expression of each protein-coding gene by CS; and/or

(c) FSM causing FSP formation is observed in less than 2% of normal tissues in a cohort of subjects without cancer,

wherein the amino acid sequence of one or more or all of such FSPs that meet the following criteria: (i) the FSP encoded by the nucleic acid providing the FSM, starting at the amino acid encoded by the FC, is 4 to 9 amino acids in length, and/or (ii) the FSP contains one or more identical contiguous stretches of 8 or more amino acids present in more than one FSP encoded by a nucleic acid with the same FSM, and/or (iii) the FSP contains one or more contiguous stretches of 8 or more amino acids also present in human wt proteins,

modified for FSP according to (i) by adding to the N-terminus of FSP from 1 to 4 amino acids of the wild-type amino acid sequence (wt) present immediately upstream of the FSP, and wherein the modified FSP (mFSP) is at least 8 amino acids in length; for FSP in accordance with (ii) modified by removing such continuous segments from all but the longest FSP, provided that the FSP is less than 4 amino acids after the removal of the continuous segment is excluded from the CVP; and/or for FSP in accordance with (iii) modified by removing such segments; modified FSPs less than 4 amino acids long are excluded from C VP after removal of the contiguous segment;

and wherein Y is at least 20, more preferably at least 35, and M is at least 5.

Preferably, at least 50% of the FSPs included in the CVP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c), or (a )+(b)+(c). Preferably, 60% of FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or (a) +(b)+(c), more preferably at least 70% of the FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c ), (b)+(c) or (a)+(b)+(c), more preferably at least 80% of the FSPs selected for CFSP meet criteria (a), (b), (c), ( a)+(b), (a)+(c), (b)+(c) or (a)+(b)+(c), more preferably at least 90% of the FSPs selected for CFSP meet the criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or (a)+(b)+(c), more preferably that at least 95% of the FSPs selected for the CFSP meet criteria (a), (b), (c), (a)+(b), (a)+(c), (b)+(c) or ( a)+(b)+(c), and most preferably at least 100% of the FSPs selected for CFSP meet criteria (a), (b), (c), (a)+(b), (a) +(c), (b)+(c) or (a)+(b)+(c).

With regard to criterion (a), it is preferable that FSP be encoded by a nucleic acid with an FSM that is observed with a CF observed for a subset of CS of a specific cancer type that is part of a collection of M different CSs that is at least 10% for at least one or all of the cancer types present in CS, preferably at least 15%, preferably at least 20%, preferably at least 25%, preferably at least 30%, more preferably at least 35%, more preferably at least at least 40% for at least one or all of the cancer types present in CS.

With regard to criterion (b), it is preferable that the average expression level of the mRNA of the FSM gene encoding FSP is in the upper 75th percentile, preferably the upper 70th percentile, preferably the upper 65th percentile, preferably the upper 60th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS. The higher the relative amount of FSP, the more likely it is that the induction of an immune response will be effective in treating or preventing forms of cancer with MSI. Solely for clarity, the phrase "upper 80th percentile" refers to all FSMs in which the level of expression is in the range including mRNAs expressed at the highest level and excluding only 19% of mRNAs expressed at the lowest level. Accordingly, the "upper 30th percentile" refers to all FSMs in which the expression level includes the mRNAs expressed at the highest level and excludes 69% of the mRNAs expressed at the lowest level.

Preferred combinations of criteria (a) and (b) are a CF of at least 5% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the mean values of mRNA expression of each protein-coding gene by CS, preferably of a specific type cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 10% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 15% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer. Other preferred combinations of criteria (a) and (b) are a CF of at least 20% and an mRNA expression level of the FSM gene in the top 75th percentile, preferably the top 70th percentile, preferably the top 65th percentile, preferably the top 60th percentile. th percentile, preferably the top 55th percentile, more preferably the top 50th percentile, more preferably the top 40th percentile, more preferably the top 30th percentile of a distribution describing the average mRNA expression of each protein-coding gene by CS, preferably a specific type of cancer.

With regard to criterion (c), it is preferable that the FSM causing FSP formation is observed in less than 2% of normal tissues in a cohort of subjects without cancer, more preferably in less than 1.9%, less than 1.8%, less than in 1.7%, less than 1.6%, less than 1.5%, less than 1.4%, less than 1.3%, less than 1.2%, less than 1 .1%, less than 1.0% or less.

According to a preferred embodiment of the CVP according to the fifth aspect of the present invention:

(i) CS are obtained from patients who have a tumor with MSI, preferably colon and rectal cancer, and/or gastric cancer, and/or endometrial cancer, more preferably colon and rectal cancer, gastric cancer and endometrial cancer; and/or

(ii) M is at least 10, preferably at least 20, preferably at least 30, preferably at least 50, more preferably at least 100, more preferably at least 200, most preferably at least 300; and/or

(iii) Y is at least 35, preferably at least 50, preferably at least 100, more preferably at least 200 and/or

(iv) the CVP contains at least 4, preferably at least 6, more preferably at least 8 amino acids of each FSP; and/or

(v) Y is selected such that the total V amino acid length of all FSPs and/or mFSPs or antigenic fragments thereof that are part of the CVP is at least 280 amino acids, preferably at least 500 amino acids, preferably at least 1000 amino acids , preferably at least 1500 amino acids, preferably at least 2000 amino acids, preferably at least 2500 amino acids, preferably at least 3000 amino acids, preferably at least 3500 amino acids, preferably at least 4000 amino acids, preferably at least 4500 amino acids, preferably at least 5000 amino acids, preferably at least 5500 amino acids, more preferably at least 6000; and/or

(vi) FSP and/or mFSP CVP selected from FSP and/or mFSP according to SEQ ID NO: 1-1087, preferably selected from FSP and/or mFSP according to SEQ ID NO: 1-209.

Preferably M is at least 30 and Y is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, preferably M is at least 50 and Y is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, M is at least 100 and Y is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least at least 300, M is at least 200 and Y is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least 300, even more preferably M is at least 300 and Y is at least 50, preferably at least 100, more preferably at least 200 and more preferably at least e 300.

According to one embodiment of the present invention, the CVP according to the fifth aspect of the present invention further comprises one or more antigens specific for a cancer other than cancer with MSI. Preferably, such antigens are also specific for one or more types of cancer being treated.

According to a preferred embodiment, at least 35, preferably at least 50, preferably at least 100, more preferably at least 200, more preferably at least 209 FSP and/or mFSP CVP, or antigenic fragments thereof, are selected from the FSP group and/or mFSP according to SEQ ID NO: 1-1087, preferably selected from the group FSP and/or mFSP according to SEQ ID NO: 1-209.

FSP and mFSP according to SEQ ID NO: 1-1087 are numbered from 1 to 1087 in descending order of relevance for the prevention or treatment of tumors with MSI, in particular colon and rectal carcinoma, gastric cancer and endometrial cancer. Specific suitable subsets comprise FSP and mFSP with the amino acid sequences of SEQ ID NOs: 1-50, 1-75, 1-100, 1-125, 1-150, 1-175, 1-200, particularly preferably 1-209, 1- 225, 1-250, 1-275, 1-300, 1-325, 1-350 1-375, 1-400, 1-450, 1-500, 1-550, 1-600, 1-650, 1 -700, 1-750, 1-800, 1-850, 1-900, 1-950, 1-1000, 1-1050 or 1-1087, or consist of them. While it is preferred that the subset contain or consist of the appropriately specified FSP and/or mFSP, one skilled in the art will appreciate that 1-10% of such FSP and/or mFSP can be omitted from the set without any significant loss. activity. Similarly, and as implied by the expression "comprising", a subset that contains, for example, the amino acid sequences of SEQ ID NO: 1-200 may further contain 1-100 or more FSP or mFSP SEQ ID NO: 201-1087 and/or one or more other FSPs or mFSPs or antigens specific to cancers other than those with MSI.

According to a preferred embodiment of the CVPs according to the fifth aspect of the present invention, the CVP FSPs were sequentially selected from the CFSP, and at each selection step, a new FSP or antigenic fragment thereof is selected from the CFSP in order to increase the CAFSPL to reach the threshold value in the maximum number of cancer samples. whose CAFSPL is below such a threshold, and optionally, if more than one FSP raises the CAFSPL for the maximum number of cancer samples in which the CAFSPL is still below the threshold, the FSP with the highest score is selected.

According to a preferred embodiment of the CVP, in accordance with the fifth aspect of the present invention:

(i) CAFSPL for each cancer sample is determined by summing the amino acid length of the FSPs that are already part of the CVP and the new FSP from CFSPP for which the corresponding FSMs are present in the cancer sample; and/or

(ii) a threshold value is determined separately for each subset of samples from the CS that are related to a particular type of cancer; and/or

(iii) the score is defined as the product of the length in amino acids of the FSP and the overall frequency of occurrence with which the FSM, causing the formation of FSP, is observed in CS; and/or

(iv) adding new FSPs continues until there are no available FSPs whose inclusion in the CVP would increase the CAFSPL of any cancer sample whose CAFSPL is still below Threshold (TV); and/or

(v) wherein the FSPs arising from the same FSM are treated as one FSP with the cumulative score calculated as the sum of the scores for the individual FSPs.

According to a preferred embodiment, a CVP according to the fifth aspect of the present invention is provided,

(i) where CAFSPL for each cancer sample is determined by summing the amino acid length of the FSPs that are already part of the CVP and the new FSP from the CFSP for which the corresponding FSMs are present in the cancer sample; and/or

(ii) where the threshold value is determined separately for each subset of samples from the CS that belong to a particular type of cancer; and/or

(iii) where the score is defined as the product of the length in amino acids of the FSP and the total frequency of occurrence of FSM, causing the formation of FSP, excluding FSM in cancer samples of a cancer type for which the CF is below 5%, more preferably below 4% and more preferably below 3% ; and/or

(iv) where the subset of cancer samples contains all cancer samples from tumor types in which FSM is present with a CF greater than or equal to 5%, more preferably greater than or equal to 10%, greater than or equal to 15%; and/or

(v) where FSP resulting from FSM with an overall frequency of occurrence below 5%, more preferably below 4% and more preferably below 3%, are excluded from the selection; and/or

(vi) where the addition of new FSPs continues until there are no available FSPs whose inclusion in the CVP would increase the CAFSPL of any cancer sample whose CAFSPL is still below TV, or the sum length of all FSPs present in the CVP , will not reach the maximum value of V amino acids; and/or

(vii) and where the FSPs arising from the same FSM are treated as one FSP with the cumulative score calculated as the sum of the scores for the individual FSPs.

One skilled in the art will appreciate that the CVP according to the fifth aspect can be constructed using the method according to the first and second aspects and, accordingly, the selection steps and criteria described in relation to the first and second aspects can be similarly applied to characterize the CVP according to fifth aspect. Accordingly, in a preferred embodiment, at least 50%, more preferably at least 60%, at least 70%, at least 80%, at least 90% meet criteria (i)+(iii)+(vi). , at least 95% FSP.

Thus, it is preferred that at least 50% of the FSPs are selected, preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi) , (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more than preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii )+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+ (vii) or

(i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), ( i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i) +(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+ (vii), more preferably at least 60% of the FSPs are selected preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+( vi), (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii) , more preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+ (ii)+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi )+(vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v) +(vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+( vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v )+(vi)+(vii), more preferably th Typically at least 70% of the FSPs were selected, preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi), (i)+ (iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria ( i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii) +(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or ( i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), (i )+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+ (iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+( vii), more preferably at least 80% of the FSPs are selected, preferably based on criteria (i)+(iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi ), (i)+(iii)+(iv)+(vi), (i)+(iii)+(v)+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i)+(ii)+( iii)+(v)+(vi), (i)+(ii)+(iii)+(vi)+(vii), (i)+(iii)+(iv)+(v)+(vi) , (i)+(iii)+(iv)+(vi)+(vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+ (ii)+(iii)+(iv)+(v)+(vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii )+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i) +(ii)+(iii)+(iv)+(v)+(vi)+(vii), more preferably at least 90% of the FSPs are selected preferably based on criteria (i)+(iii)+( vi), more preferably based on criteria (i)+(ii)+(iii)+(vi), (i)+(iii)+(iv)+(vi), (i)+(iii)+(v )+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi), (i) +(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii)+(vi)+(vii), (i)+(iii)+(iv)+( v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or (i)+(iii)+(v)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), (i)+(ii)+(iii)+(iv)+(vi)+(vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+(vii) and most preferably 100% FSPs are selected preferably based on criteria (i)+( iii)+(vi), more preferably based on criteria (i)+(ii)+(iii)+(vi), (i)+(iii)+(iv)+(vi), (i)+(iii )+(v)+(vi) or (i)+(iii)+(vi)+(vii), more preferably based on criteria (i)+(ii)+(iii)+(iv)+(vi) , (i)+(ii)+(iii)+(v)+(vi), (i)+(ii)+(iii)+(vi)+(vii), (i)+(iii)+( iv)+(v)+(vi), (i)+(iii)+(iv)+(vi)+(vii) or (i)+(iii)+(v)+(vi)+(vii) , more preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi), (i)+(ii)+(iii)+(iv)+(vi)+ (vii), (i)+(ii)+(iii)+(v)+(vi)+(vii) or (i)+(iii)+(iv)+(v)+(vi)+(vii ), most preferably based on criteria (i)+(ii)+(iii)+(iv)+(v)+(vi)+(vii).

According to a preferred embodiment of the CVP, according to the fifth aspect of the present invention, TV is:

(a) TV is at least 400 amino acids, preferably at least 600 amino acids, more preferably at least 800 amino acids; or

(b) TV is:

(i) at least 400 amino acids, preferably at least 600 amino acids, more preferably at least 800 amino acids for colon and rectal cancer and gastric cancer; And

(ii) at least 200 amino acids, preferably at least 300 amino acids, more preferably at least 400 amino acids for endometrial cancer.

According to a preferred embodiment of the CVP, according to the fifth aspect of the present invention, the CVP peptides are separated, or at least two FSPs and/or mFSPs are contained in one or more polypeptides. When linking two or more FSPs and/or mFSPs to form a polypeptide, it is preferred that the linkage is direct via a peptide bond, i.e. no amino acid linker.

It is also preferred that before linking two FSPs and/or mFSPs within a polypeptide, it is assessed whether the resulting border sequence contains one or more contiguous stretches of 8 or more amino acids also present in human wt proteins. If so, then the two peptides are not related in this way. Accordingly, the resulting polypeptide from linked FSPs and/or mFSPs will not contain one or more contiguous stretches of 8 or more amino acids also present in human wt proteins. Depending on the total length of the FSP and/or mFSP contained in the CVP, the FSP and/or mFSP may be contained in two, three, four, five, six or more polypeptides.

The present inventors have assembled 209 FSPs and mFSPs of SEQ ID NO: 1-209 or antigenic fragments thereof into four polypeptides and have found that this is a particularly suitable representation of such antigens. Preferred four polypeptide arrangements of FSP and mFSP SEQ ID NO: 1-209 consist of or include the following four polypeptides with amino acid sequences according to SEQ ID NO: 1088-1091 (Layout A), SEQ ID NO: 1092-1095 ( layout B), SEQ ID NO: 1155-1158 (layout C) or SEQ ID NO: 1159-1162 (layout D), or consists of them.

Preferably, in subsequent cycles of administration of the same CVP, i.e. containing the same FSP and mFSP, polypeptides containing FSP and mFSP, for example, in accordance with SEQ ID NO: 1-209, were located in a different order. This minimizes unwanted immune responses to linked epitopes. For example, it is preferable to combine CVP administration as Polypeptide Layout A with Polypeptide Layout B, Layout A with Layout C, Layout A with Layout D, Layout B with Layout C, Layout B with Layout D, Layout C with Layout D, Layout A with Layout B and C layout, A-layout with B-layout and D-layout, B-layout with C-layout and D-layout, A-layout with B-layout, C-layout and D-layout.

Accordingly, the present invention also provides 2, 3, 4, 5, 6 or more different polypeptide arrangements, with each set containing one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides. containing the same FSP and mFSP or their antigenic fragments, or consisting of them, i.e. are the same CVP. The difference between each set thus lies not in the amino acid sequence of the FSP, mFSP, or antigenic fragments thereof, but in the location of the FSP, mFSP, or antigenic fragments thereof in the corresponding number of polypeptides. It is particularly preferred that a given CVP be provided in two different arrangements and contain one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides, most preferably 4 polypeptides containing the same FSPs. and mFSP or antigenic fragments thereof, or consisting of them, in three different layouts and contained one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides, most preferably 4 polypeptides containing the same FSP and mFSP or antigenic fragments thereof, or consisting of them, in four different layouts and contained one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides, most preferably 4 polypeptides containing or consisting of the same FSP and mFSP or antigenic fragments thereof, in five different arrangements and containing one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides, most preferably 4 polypeptides containing the same F SP and mFSP or their antigenic fragments, or consisting of them. Preferably, each individual arrangement is distinguished from other arrangements by different boundary sequences, i.e. the two arrangements do not provide for a link between the same N-C-terminal link of one FSP or mFSP to the next FSP or mFSP.

Each formulation will typically be administered to the patient separately, and thus different formulations may also be considered separate formulations of the same CVP.

According to a preferred embodiment, a CVP according to a fifth aspect of the present invention is provided, wherein one or more FSPs and/or mFSPs or polypeptides comprising two or more FSPs and/or mFSP CVPs are linked, preferably via peptide bonds, to one or more of the following, which increase the immunogenicity of CVP: the sequence of the invariant chain or its fragment; tissue plasminogen activator; PEST sequence; boxing destruction of cyclin; ubiquitination signal; sumoylation signal; interleukin, preferably interleukin 2, interleukin 12 or interleukin 15; a checkpoint protein-specific ligand, preferably an anti-PD1 antibody or a PD1 binding fragment thereof, an anti-CTLA4 antibody or a CTLA4 binding fragment thereof, an anti-LAG3 antibody or a LAG3 binding fragment thereof, an anti-TIM3 antibody or a TIM3 binding fragment thereof.

In a preferred embodiment, a sorting signal (either a human tissue plasminogen activator signal peptide (hTPA; SEQ ID NO: 1104 or a functional fragment thereof that is sorted as hTPA) or a human invariant chain (hINV; SEQ ID NO: 1105 or a functional fragment thereof) a fragment that is sorted as hINV) is added to the N-terminus, and optionally an influenza virus HA tag sequence (SEQ ID NO: 1106) is added to the C-terminus of the polypeptides of SEQ ID NO: 1088-1091 (layout A), SEQ ID NO: 1092-1095 (layout B), SEQ ID NO: 1155-1158 (layout C) or SEQ ID NO: 1159-1162 (layout D), or consists of them.

Preferably, the amino acid sequence of the thus constructed polypeptides is shown as SEQ ID NO: 1107-1110 (layout A), SEQ ID NO: 111 1-1114 (layout B), SEQ ID NO: 1171-1174 (layout C), and SEQ ID NO: 1179 -1182 (layout D) for hTPA and SEQ ID NO: 1115-1118 (layout A), SEQ ID NO: 1119-1122 (layout B), SEQ ID NO: 1175-1178 (layout C) and SEQ ID NO: 1183 -1186 (D layout) for hINV.

According to the sixth aspect, the present invention relates to a collection of nucleic acids encoding CVP according to the fifth aspect of the present invention. The nucleic acid may preferably be DNA, RNA or RNA modified to increase its serum half-life.

For the above reasons with respect to CVP, it is preferable to use 2, 3, 4, 5, 6, 7, 8, 9, 10 or more, preferably 4 different arrangements 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptides containing FSP, mFPS or antigenic fragments thereof. Accordingly, the collection of nucleic acids encoding the corresponding number of polypeptides is characterized by different layouts. In a preferred embodiment, the collection of nucleic acids encoding FSB and/or mFSB encodes four polypeptides according to SEQ ID NO: 1088-1091 (layout A), SEQ ID NO: 1092-1095 (layout B), SEQ ID NO: 1155 -1158 (layout C) or SEQ ID NO: 1159-1162 (layout D) and is codon-optimized based on human codon frequency.

When the CVP is administered in the form of a collection of nucleic acids encoding that CVP, it is preferred to use 2, 3, 4, 5, 6 or more different arrangements of nucleic acids, with each arrangement providing one or more, preferably 2, 3, 4, 5, 6 , 7, 8, 9 or 10 or more nucleic acids encoding polypeptides containing or consisting of the same FSP and mFSP or antigenic fragments thereof, i.e. encodes the same CVP. It is particularly preferred that this collection of nucleic acids encoding the CVP according to the fourth or fifth aspect of the present invention is provided in two different arrangements and contains one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more nucleic acids encoding polypeptides, most preferably 4 nucleic acids encoding polypeptides containing the same FSP and mFSP or antigenic fragments thereof, or consisting of them, in three different arrangements and contained one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptide-encoding nucleic acids, most preferably 4 polypeptide-encoding nucleic acids containing or consisting of the same FSP and mFSP or antigenic fragments thereof, in four different arrangements and contains one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more nucleic acids encoding polypeptides, most preferably 4 nucleic acids encoding e polypeptides containing or consisting of the same FSP and mFSP or antigenic fragments thereof, in five different arrangements and containing one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more than 4 nucleic acids encoding polypeptides, most preferably 4 nucleic acids encoding polypeptides, containing or consisting of the same FSP and mFSP or antigenic fragments thereof, or in six different arrangements and contained one or more, preferably 2, 3, 4, 5, 6, 7, 8, 9 or 10 or more polypeptide-encoding nucleic acids, most preferably 4 polypeptide-encoding nucleic acids containing or consisting of the same FSP and mFSP or antigenic fragments thereof.

Additional examples of such collections of nucleic acids encoding FSP and mFSP SEQ ID NO: 1-209 encoding the same CVP but with a different layout are shown in SEQ ID NO: 1123-1126 for layout A and SEQIDNO: 1187-1190 for layout C with hTPA; SEQ ID NO: 1127-1130 for layout B and SEQ ID NO: 1191-1194 for layout D with hTPA; SEQ ID NO: 1131-1134 for layout A and SEQ ID NO: 1195-1198 for layout C with hINV and SEQ ID NO: 1135-1138 for layout B and SEQ ID NO: 1199-1202 for layout D with hINV.

In a preferred embodiment, the Kozak sequence (CGCGACTTCGCCGCC) can be placed directly upstream of the start codon of the collection nucleic acid according to the fourth or sixth aspect of the present invention to ensure efficient translation initiation, and the TAA stop codon is placed below the HA tag. Finally, it is preferred that the collection of nucleic acids contains two flanking segments containing unique restriction sites to facilitate subcloning of cassettes added at the 5' and 3' end of the nucleotide sequence, respectively (FIG. 7).

A collection of nucleic acids according to the fourth and sixth aspects of the present invention may also be contained in an expression cassette.

According to the seventh aspect, the present invention relates to a collection of one or more expression vectors, each of which contains the entire collection of nucleic acids according to the fourth or sixth aspect of the present invention, or part thereof, and the complete collection of expression vectors contains the entire collection of nucleic acids according to the fourth or sixth aspect of the present inventions, i.e. the collection of expression vectors contains nucleic acids encoding all FSPs, mFSPs, or antigenic fragments thereof, of a given CVP.

Preferably, the collection of expression vectors contains one or more elements that increase the immunogenicity of the expression vector. Preferably, such elements are expressed as a fusion product with FSP, mFSP or antigenic fragments thereof, or encoded by another nucleic acid contained in a vector, preferably in an expression cassette.

In a preferred embodiment, the element that enhances the immunogenicity of CVP is selected from the group consisting of an invariant chain sequence or an immunostimulatory fragment thereof; tissue plasminogen activator; PEST sequences; boxing destruction of cyclin; ubiquitination signal; sumoylation signal; interleukin, preferably interleukin 2, interleukin 12 or interleukin 15; checkpoint protein-specific ligand, preferably an anti-PD1 antibody or PD1-binding fragment thereof, an anti-CTLA4 antibody or CTLA4-binding fragment thereof, an anti-LAG3 antibody or LAG3-binding fragment thereof, an anti-TIM3 antibody or a THM3-binding fragment thereof .

According to a preferred embodiment of the collection of expression vectors according to the seventh aspect, each expression vector from the collection is independently selected from the group consisting of a plasmid; cosmids; RNA; RNA formulated with an adjuvant; RNA compiled into liposomal particles; self-amplifying RNA (SAM); SAM formulated with an adjuvant; SAM formulated into liposomal particles; viral vector; preferably an alphavirus vector, a Venezuelan equine encephalitis virus (VEE) vector, a Sindbis virus (SIN) vector, a Semliki Forest Virus (SFV) vector, also preferably a replication-competent or replication-incompetent adenovirus vector, preferably chimpanzee or bonobo or gorilla derived poxvirus, vaccinia or modified vaccinia Ankara (MVA) vector, simian or human cytomegalovirus (CMV) vector, lymphocytic choriomeningitis virus vector ( LCMV), retrovirus or lentivirus vectors. Preferably, all expression vectors used for one collection are of the same type, eg replication-incompetent adenovirus vectors.

The most preferred expression vectors are adenovirus vectors, in particular adenovirus vectors derived from humans or non-human hominids. Preferred members of the hominids from which adenoviruses are derived are chimpanzees (Pan), gorillas (Gorilla) and orangutans (Pongo), preferably bonobos (Pan paniscus) and common chimpanzees (Pan troglodytes). Typically, naturally occurring adenoviruses of a non-human hominid are isolated from fecal samples of the corresponding hominid. The most preferred vectors are non-replicating adenovirus based vectors hAd5, hAd11, hAd26, hAd35, hAd49, ChAd3, ChAd4, ChAd5, ChAd6, ChAd7, ChAd8, ChAd9, ChAd10, ChAd11, ChAd16, ChAd17, ChAd19, ChAd20, ChAd22, ChAd24, ChAd26, ChAd30, ChAd31, ChAd37, ChAd38, ChAd44, ChAd55, ChAd63, ChAd73, ChAd82, ChAd83, ChAdl46, ChAdl47, PanAd1, PanAd2 and PanAd3 or Ad4 and Ad7 replication competent vectors. The human adenoviruses hAd4, hAd5, hAd7, hAd11, hAd26, hAd35 and hAd49 are well known in the art. Vectors based on naturally occurring ChAd3, ChAd4, ChAd5, ChAd6, ChAd7, ChAd8, ChAd9, ChAd10, ChAd11, ChAd16, ChAd17, ChAd19, ChAd20, ChAd22, ChAd24, ChAd26, ChAd30, ChAd31, ChAd37, ChAd38, ChAd34, and ChAd82 are described in detail in WO 2005/071093. Vectors based on the naturally occurring PanAd1, PanAd2, PanAd3, ChAd55, ChAd73, ChAd83, ChAd146 and ChAd147 are described in detail in WO 2010/086189.

In a specific embodiment, the adenoviral vector is Gad20 (also referred to as GADNOU20, SEQ ID NO: 1219) or a vector derived therefrom. In one embodiment, "derived from it" means that the adenoviral vector has at least 85% and preferably at least 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with SEQ ID NO: 1219. According to the preferred embodiment, however, this means the following:

the adenoviral vector encodes an adenovirus hexon protein comprising: (i) a first HVR1 hypervariable region containing the amino acid (aa) sequence encoded by nucleotides 19386-19472 of SEQ ID NO: 1219, or a variant thereof characterized by at least 85% identity to aa sequences, and not containing A, and preferably containing V at position aa27,

(i) a second hypervariable region of HVR2 containing aa sequence encoded by nucleotides 19527-19571 of SEQ ID NO: 1219, or a variant thereof, characterized by at least 85% identity to aa sequences, and not containing L, and preferably containing I at position aa 1 ,

(iii) a third hypervariable region of HVR3 containing the aa sequence encoded by nucleotides 19623-19643 of SEQ ID NO: 1219, or a variant thereof, characterized by at least 85% identity to aa sequences, and not containing V, and preferably containing A at position aa 7 ,

(iv) a fourth hypervariable region of HVR4 containing an aa sequence encoded by nucleotides 19737-19772 of SEQ ID NO: 1219, or a variant thereof, characterized by at least 85% identity of aa sequences,

(v) a fifth hypervariable region of HVR5 containing the aa sequence encoded by nucleotides 19794-19838 of SEQ ID NO: 1219, or a variant thereof, characterized by at least 85% identity of the aa sequences,

(vi) a sixth HVR6 hypervariable region comprising an aa sequence encoded by nucleotides 19908-19934 of SEQ ID NO: 1219, or a variant thereof having at least 85% aa sequence identity, and

(vii) a seventh hypervariable region of HVR7 containing the aa sequence encoded by nucleotides 20259-20336 of SEQ ID NO: 1219, or a variant thereof characterized by at least 85% aa sequence identity, without I and preferably with V at position aa 1.

In a preferred embodiment, the HVR variants share at least 90% and more preferably at least 95% sequence identity. As an alternative to definition using a percentage level of sequence identity, HVR variants can be defined as having a certain number of mutations in an amino acid compared to the encoded sequence. The number of mutations in this case is as follows: instead of at least 85% sequence identity, up to 4 mutations in HVR1, up to 2 mutations in HVR2, up to 1 mutation in HVR3, up to 1 mutation in HVR4, up to 2 mutations in HVR5, up to 1 mutations in HVR6 and up to 3 mutations in HVR7; instead of at least 90% sequence identity, up to 2 mutations in HVR1, up to 1 mutation in HVR2, up to 1 mutation in HVR3, and preferably no mutations, up to 1 mutation in HVR4, up to 1 mutation in HVR5, up to 1 mutation in HVR6, and preferably no mutations, and up to 2 mutations in HVR7; instead of at least 95% sequence identity, up to 1 mutation in HVR1, up to 1 mutation in HVR2, and preferably no mutation, up to 1 mutation in HVR3, and preferably no mutation, up to 1 mutation in HVR4, and preferably no mutation, up to 1 mutations in HVR5, and preferably no mutation, up to 1 mutation in HVR6, and preferably no mutation, and up to 1 mutation in HVR7.

As is known in the art, for example, from Bradley et al. (J Virol., 2012 Jan;86(2): 1267-72), adenovirus neutralizing antibodies target the hexon hypervariable regions, and by replacing adenovirus HVR regions with serum abundant elements, such adenovirus can avoid the action of the immune system in the body host that elicits an immune response. Thus, although the aforementioned HVRs can be used with the corresponding hexon proteins defined below, they are useful independently of such hexon proteins, as well as the penton proteins and fibril proteins mentioned below, namely by replacing the hexon HVR in another adenovirus with other hexon proteins, penton and/or fibrils.

In a preferred embodiment, the hexon protein comprises the amino acid sequence encoded by nucleotides 18981-21845 of SEQ ID NO: 1219, or a variant thereof having at least 85% sequence identity.

In a preferred embodiment, the hexon variants share at least 90% and preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity. As an alternative to being defined by a percentage level of sequence identity, hexon variants can be defined as having a certain number of mutations in an amino acid compared to the encoded sequence. The number of mutations in this case is as follows: instead of at least 85% sequence identity, up to 143 mutations; instead of at least 90% sequence identity, up to 95 mutations; instead of at least 95% sequence identity, up to 47 mutations; instead of at least 96% sequence identity, up to 38 mutations; instead of at least 97% sequence identity, up to 28 mutations; instead of at least 98% sequence identity, up to 19 mutations; instead of at least 99% sequence identity, up to 9 mutations. It should be understood that hexon variants do not share less sequence identity or contain more mutations in their HVRs than defined for the respective HVRs above.

In one embodiment, the adenoviral vector further encodes an adenovirus penton protein comprising the amino acid sequence encoded by nucleotides 14021-15973 of SEQ ID NO: 1219, or a variant thereof having at least 85% sequence identity. In a preferred embodiment, the pentone variants have at least 90% and preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity relative to the encoded sequence. As an alternative to being defined by a percentage level of sequence identity, pentone variants can be defined as having a certain number of amino acid mutations. The number of mutations in this case is as follows: instead of at least 85% sequence identity, up to 97 mutations; instead of at least 90% sequence identity, up to 65 mutations; instead of at least 95% sequence identity, up to 32 mutations; instead of at least 96% sequence identity, up to 26 mutations; instead of at least 97% sequence identity, up to 19 mutations; instead of at least 98% sequence identity, up to 13 mutations; instead of at least 99% sequence identity, up to 6 mutations.

Preferably the pentone variants do not contain D and preferably contain G at amino acid position 289 and do not contain D and preferably contain N at amino acid position 341.

According to another embodiment, the adenoviral vector additionally (i.e. next to the hexon protein and possibly the penton protein) encodes an adenovirus fibril protein containing the amino acid sequence encoded by nucleotides 32163-33956 of SEQ ID NO: 1219, or a variant thereof characterized by at least least 85% sequence identity. In a preferred embodiment, the fibril variants share at least 90% and preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity. As an alternative to being defined by a percentage level of sequence identity, fibril variants can be defined as having a certain number of mutations in an amino acid compared to the encoded sequence. The number of mutations in this case is as follows: instead of at least 85% sequence identity, up to 89 mutations; instead of at least 90% sequence identity, up to 59 mutations; instead of at least 95% sequence identity, up to 29 mutations; instead of at least 96% sequence identity, up to 23 mutations; instead of at least 97% sequence identity, up to 17 mutations; instead of at least 98% sequence identity, up to 11 mutations; instead of at least 99% sequence identity, up to 5 mutations.

Preferably, the fibril variants do not contain A and preferably contain P at amino acid position 181, do not contain V and preferably contain I at amino acid position 474 and/or do not contain an S insert, and preferably do not contain an amino acid insert between amino acid positions 4 and 5.

According to another embodiment, the adenoviral vector additionally (i.e. adjacent to the hexon and possibly pentone and/or fibril protein) encodes VA-RNA II, which is a non-coding RNA containing the nucleotide sequence according to nucleotides 10724-10897 of SEQ ID NO: 1219 or a nucleotide sequence variant having at least 85% sequence identity. Alternatively, or in addition, it may encode VA RNA I, which is a non-coding RNA containing a nucleotide sequence according to nucleotides 10492-10659 of SEQ ID NO: 1219 or a nucleotide sequence variant having at least 85% sequence identity. In a preferred embodiment, the VA RNA variants have at least 90% and preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity. As an alternative to definition using a percentage level of sequence identity, VA RNA variants can be defined as having a certain number of mutations at a nucleotide. The number of mutations in this case is as follows: instead of at least 85% sequence identity, up to 25 mutations in VA RNA I and up to 26 mutations in VA RNA II; instead of at least 90% sequence identity, up to 16 mutations in VA RNA I and up to 17 mutations in VA RNA II; instead of at least 95% sequence identity, up to 8 mutations in any VA RNA; instead of at least 96% sequence identity, up to 6 mutations in any VA RNA; instead of at least 97% sequence identity, up to 5 mutations in any VA RNA; instead of at least 98% sequence identity, up to 3 mutations in any VA RNA; instead of at least 99% sequence identity, up to 1 mutation in any VA RNA.

Preferably the variant VA RNA II (a) does not contain C at position 79 and/or does not contain A at position 80 and preferably contains T at position 79 and/or G at position 80 and (b) does not contain A at position 81 and preferably contains a G at position 81. The variant VA RNA I preferably does not contain a G at position 80 and preferably contains an A at position 80.

VA RNA in accordance with the present invention provides increased production of adenovirus.

Preferably, the adenoviral vector further contains other adenovirus genes and nucleotide segments that are adjacent to the hexon, penton and/or fibril gene in the adenovirus genome, using SEQ ID NO: 1219 as a reference. In particular, it is preferred that the adenoviral vector also contains the sequences required to package the polynucleotide into an adenoviral particle.

In general, it is preferred that the adenoviral vector contains at least one of the following:

(a) the 5' end of the adenovirus, preferably the 5' inverted terminal repeat of the adenovirus;

(b) an adenovirus E1a region, or a fragment thereof, selected from the 13S, 12S, and 9S regions;

(c) an adenovirus E1b region, or a fragment thereof, selected from the group consisting of regions encoding small T antigen, large T antigen, and region IX;

(d) the VA region of adenovirus RNA; or a fragment thereof selected from the group consisting of VA RNA I and VA RNA II regions;

(e) the E2b region of the adenovirus; or a fragment thereof selected from the group consisting of a small pTP coding region, a polymerase coding region, and an IVa2 region;

(f) an adenovirus L1 region or a fragment thereof, said fragment encoding an adenoviral protein selected from the group consisting of a 28.1 kD protein, a polymerase, an agnoprotein, a 52/55 kD protein, and protein IIIa;

(g) an L2 region of an adenovirus or a fragment thereof, said fragment encoding an adenovirus protein selected from the group consisting of Penton protein as defined above, protein VII, V and X;

(h) an adenovirus L3 region or fragment thereof, said fragment encoding an adenoviral protein selected from the group consisting of protein VI, a hexon protein as defined above, and an endoprotease;

(i) an adenovirus E2a region or a fragment thereof, said fragment encoding an adenovirus protein comprising a DBP protein;

(j) an adenovirus L4 region or a fragment thereof, said fragment encoding an adenoviral protein selected from the group consisting of a 100 kDa protein, a 22 kDa protein homologue, a 33 kDa protein homologue, and protein VIII;

(k) an E3 adenovirus region or fragment thereof selected from the group consisting of E3 ORF1, E3 ORF2, E3 ORF3, E3 ORF4, E3 ORF5, E3 ORF6, E3 ORF7, E3 ORF8 and E3 ORF9;

(l) an adenovirus L5 region or a fragment thereof, said fragment encoding a fibril protein as defined above;

(m) an E4 adenovirus region, or a fragment thereof, selected from the group consisting of E4 ORF6/7, E4 ORF6, E4 ORF5, E4 ORF4, E4 ORF3, E4 ORF2, and E4 ORF1;

(n) the 3' end of the adenovirus, preferably the 3' inverted terminal repeat of the adenovirus; and/or

(o) El region of the adenovirus.

These elements may be derived from the same adenovirus according to SEQ ID NO: 1219, or from a different adenovirus, in particular from a different species, eg human adenovirus, to form a chimeric adenovirus.

In some embodiments of the adenoviral vector, it may be desirable that it does not contain one or more of the genomic regions described above (such as in (a)-(m)). In particular, it may not contain the E1, E3 and/or E4 regions, and/or may contain an adenovirus gene that includes a deletion and/or mutation that renders at least one gene non-functional. In preferred embodiments, suitable regions of the adenovirus are modified so that they do not include the aforementioned region(s)/gene(s), or so as to make the selected region(s)/gene(s) non-functional. One way to render them non-functional is to introduce one or more artificial stop codons (eg, TAA) into the open reading frame of these genes. Techniques for making a virus replication defective are well known in the art (see, for example, Brody et al, 1994 Ann NY Acad Sci., 716: 90-101). The deletion can make room for the insertion of transgenes, preferably within an expression cassette, such as a minigene cassette. In addition, deletions can be used to generate adenovirus vectors that are unable to replicate without the use of a packaging cell line or helper virus, as is well known in the art. Such a recombinant adenovirus containing one or more of these gene/region deletions or loss-of-function mutations may provide a safer recombinant adenovirus, for example for gene therapy or vaccination.

Although an adenoviral vector may not contain at least one genomic region/gene as described herein (such as the E1, E3 and/or E4 region), in particular E1A, E1B, E2A, E2 B, E3 ORF1, E3 ORF2, E3 ORF3, E3 ORF4, E3 ORF5, E3 ORF6, E3 ORF7, E3 ORF8, E3 ORF9, E4 ORF6/7, E4 ORF6, E4 ORF5, E4 ORF4, E4 ORF3, E4 ORF2 and/or E4 ORF1, preferably E1A, E1B, E2A, E2B, E3 and/or E4, and/or may contain an adenovirus gene that includes a deletion and/or mutation that makes at least one gene non-functional, it is desirable that the E1a and/or E1b region remain intact . Such an intact E1 region may be located at its native location in the adenovirus genome, or may be placed at a deletion site in the native adenovirus genome (eg, in the E3 region).

In a preferred embodiment, the adenoviral vector further encodes one or more, preferably all, of the following adenoviral proteins: protein VI, protein VIII, protein IX, protein IIIa, and protein IVa2.

One of ordinary skill in the art related to adenoviruses is well aware of how to determine the open reading frames that encode the above adenovirus proteins. He also knows the structure of adenovirus genomes and will be able to easily transfer the individual adenovirus and ORF regions described herein to any adenovirus genome.

The term "hexon protein" refers to the hexon(II) protein contained in the adenovirus. The hexon protein or a variant thereof according to the present invention has the same function as the hexon protein or fragment thereof in an infectious adenovirus virion. Thus, an adenovirus containing said hexon or a variant thereof, preferably as a capsid protein, is able to enter the host cell. A suitable method for producing hexon protein variants is described in US Patent Application 5,922,315. According to this method, at least one hexon loop of an adenovirus is replaced with at least one loop of an adenovirus of a different serotype. It can be easily determined whether the recombinant adenovirus can enter the host cell. For example, after contacting a host cell with an adenovirus, the recombinant host cell can be washed and lysed, and it can be determined whether adenoviral RNA and/or DNA is found in the host cell using, for example, a suitable hybridization probe specific for adenovirus RNA and/or DNA. Alternatively, or in addition, the host cell, after being contacted with the recombinant adenovirus, can be washed, lysed, and tested for adenovirus-specific antibodies, for example, using Western blotting. In yet another alternative, it is observed, for example in vivo, whether the host cell expresses a gene product, for example a fluorescent protein, after infection with a recombinant adenovirus that contains an appropriate expression cassette to allow expression of the gene product in the host cell.

The term "hypervariable region" (HVR) refers to domains with high sequence variability among strains located on the hydrophilic surface of the hexon protein found on the outside of the viral capsid. They are the main determinants for neutralizing antibodies. HVR can be identified, for example, by alignment with sequences of other hexon proteins.

By "pentone protein of adenovirus" is meant a protein that is the base of pentone (III) contained in the composition of adenovirus. The pentone protein of adenovirus is characterized by the fact that it is localized in the corners of the icosahedral symmetry of the capsid. The Penton protein or a variant thereof according to the present invention has the same function as the Penton protein in the infectious adenovirus virion. Thus, an adenovirus containing said pentone or a variant thereof, preferably as a capsid protein, is able to enter the host cell, which can be tested as described above. In addition, the functional pentone is characterized by affinity for the adenovirus fibril protein. One of ordinary skill in the art is well aware of how to test affinity scores for protein-protein interactions. To determine whether the first protein is able to bind the second protein, for example, an assay using a yeast two-hybrid system or a biochemical assay such as co-precipitation assay, enzyme immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS) assay can be used. ) or analysis based on the phenomenon of surface plasmon resonance. In the case of a co-precipitation or surface plasmon resonance assay, it is advantageous to fuse at least one of the proteins with an affinity tag, such as a HIS tag, GST tag, or other, as is well known in the field of biochemistry.

The term “fibril protein” refers to the fibril head (IV) protein contained within the adenovirus. The fibril protein or a variant thereof according to the present invention has the same function as the fibril protein or fragment thereof in an infectious adenovirus virion. Thus, an adenovirus containing said fibril or fibril variant, preferably as a capsid protein, is able to enter the host cell, which can be tested as described above. In addition, the functional fibril protein has an affinity for the adenovirus penton protein. Also, the functional adenovirus fibril protein in its glycosylated form is capable of trimerization. Thus, it is also preferred that the variant be able to glycosylate and/or form a trimer. Affinity, including trimerization, can be tested as described above, and glycosylation assays are also well known in the art.

"VA (virus-associated) RNA" is a type of non-coding RNA found in adenovirus. It plays a role in the regulation of translation. There are two copies of this RNA, called VAI or VA RNA I and VAII or VA RNA II. The two VA RNA genes are separate genes in the adenovirus genome. VA RNA I is the main molecule, while VA RNA II is expressed at a lower level. Neither transcript is polyadenylated and both are transcribed by Po1III.

The term "identity" or "identical" in the context of a polynucleotide, polypeptide or protein sequence refers to the number of residues in two sequences that are identical when aligned for maximum match. In particular, the percent sequence identity of two sequences, whether nucleic acid sequences or amino acid sequences, is the number of exact matches between two aligned sequences divided by the length of the shorter sequence multiplied by 100. Alignment tools that can be used to align two sequences , are well known to the person skilled in the art and may be available for example on the World Wide Web, for example Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/) for polypeptide alignments or MUSCLE ( http://www.ebi.ac.uk/Tools/msa/muscle/) or MAFFT (http://www.ebi.ac.uk/Tools/msa/maffi/) for polynucleotide alignments, or WATER (http: //www.ebi.ac.uk/Tools/psa/emboss_water/) for polynucleotide and polypeptide alignments. Alignments of two sequences can be performed using default parameters, e.g. for MAFFT preferred: template: Blosum62, gap opening penalty 1.53, gap extension penalty 0.123, for WATER aligned polynucleotides preferred: template: DNAFULL, opening penalty gap: 10.0, gap extension penalty 0.5, and for WATER-aligned polypeptides preferably: MATRIX: BLOSUM62, gap opening penalty: 10.0, gap extension penalty: 0.5. Those skilled in the art will appreciate that gapping in any sequence may be required to obtain a satisfactory alignment. The "best sequence alignment" is defined as the alignment that produces the most aligned identical residues with the fewest gaps. Preferably, it is a global alignment that includes every residue in every sequence in the alignment.

The term "variant" in relation to a polypeptide generally refers to a modified version of a polypeptide, for example, by mutation, due to which one or more amino acids of the polypeptide can be deleted, inserted, modified and/or replaced. In general, the variant is functional, which means that an adenovirus containing a functional variant is capable of infecting a host cell. More specific functions are defined in this document and take precedence over the general definition. "Mutation" or "amino acid mutation" may be an amino acid substitution, deletion and/or insertion ("and" may be used if more than one mutation occurs). Preferably it is a substitution (ie a conservative or non-conservative amino acid substitution), more preferably a conservative amino acid substitution. In some embodiments, the replacement also includes replacing a naturally occurring amino acid with a non-naturally occurring amino acid. A conservative substitution involves replacing an amino acid with another amino acid with a chemical property similar to that of the amino acid to be replaced. Preferably the conservative substitution is a substitution selected from the group consisting of:

(i) substitution of the basic amino acid for another, different basic amino acid;

(ii) replacing an acidic amino acid with a different acidic amino acid;

(iii) replacing an aromatic amino acid with a different aromatic amino acid;

(iv) replacing the non-polar aliphatic amino acid with another, different non-polar aliphatic amino acid; And

(v) replacing the polar uncharged amino acid with a different polar uncharged amino acid.

The basic amino acid is preferably selected from the group consisting of arginine, histidine and lysine. The acidic amino acid is preferably aspartate or glutamate. The aromatic amino acid is preferably selected from the group consisting of phenylalanine, tyrosine and tryptophan. The non-polar aliphatic amino acid is preferably selected from the group consisting of glycine, alanine, valine, leucine, methionine and isoleucine. The polar uncharged amino acid is preferably selected from the group consisting of serine, threonine, cysteine, proline, asparagine and glutamine. Unlike a conservative amino acid substitution, a non-conservative amino acid substitution is a substitution of one amino acid with any amino acid that is not included in the group of conservative substitutions (i)-(v) described above.

Means for determining sequence identity are described above.

The amino acids of the protein can also be modified, for example, chemically modified. For example, the side chain or free amino or carboxy terminus of an amino acid of a protein or polypeptide can be modified, for example, by glycosylation, amidation, phosphorylation, ubiquitination, and the like. Chemical modification can also take place in vivo, for example in a host cell, as is well known in the art. For example, a suitable chemical modification motif, such as a glycosylation motif, present in the amino acid sequence of a protein will cause the protein to be glycosylated. If the modification does not result in a change in the identity of the modified amino acid (eg, substitution or deletion), the modified polypeptide refers to said polypeptide, i. it is not an option as defined in this document.

The term "variant" in relation to a polynucleotide generally refers to a modified version of a polynucleotide, eg, by mutation, whereby one or more nucleotides of the polynucleotide can be deleted, inserted, modified and/or replaced. In general, the variant is functional, which means that an adenovirus containing a functional variant is capable of infecting a host cell. More specific functions are defined in this document and take precedence over the general definition. "Mutation" can be a nucleotide substitution, deletion and/or insertion ("and" can be used if more than one mutation occurs). Preferably it is a substitution, more preferably it is an amino acid substitution, most preferably a conservative amino acid substitution.

In general, it is preferred that all expression vectors in a collection of expression vectors, ie. collection of expression vectors containing nucleic acids encoding all FSPs, mFSPs and their antigenic fragments of the corresponding CVP were of the same type, for example, were replication-competent adenovirus. According to one embodiment of the present invention, which uses different layouts of the same CVP or nucleic acids encoding it, each arrangement will be contained in a separate collection of expression vectors. Thus, each arrangement may be contained in the same expression vector or in different expression vectors. The latter option is preferred in cases where the expression vector itself is antigenic in the patient. Thus, changing the type of expression vector between two or more repeated administrations of the same CVP, preferably in different arrangements, increases the likelihood that the CVP will be expressed in a patient.

The present invention also relates to a CVP according to the fourth or fifth aspect of the present invention, a collection of nucleic acids according to the fourth or sixth aspect of the present invention, or a collection of expression vectors according to the seventh aspect of the present invention for use in medicine.

The present invention also relates to a pharmaceutical composition containing a CVP according to the fourth or fifth aspect of the present invention, a collection of nucleic acids according to the fourth or sixth aspect of the present invention, or a collection of expression vectors according to the seventh aspect of the present invention.

In the case where different arrangements of the same CVP are used, nucleic acids encoding such different arrangements of the same CVP or expression vectors containing such different arrangements of nucleic acids are used using a homologous or heterologous prime-boost regimen (see . below), the term "pharmaceutical composition" also encompasses two physically separated compositions that allow separate administration of the same CVP, nucleic acids encoding such different arrangements of the same CVP, or expression vectors containing such nucleic acids with different layouts.

According to preferred embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier and/or excipient and optionally one or more additional active substances. Preferably, the composition according to the fifth aspect contains a therapeutically effective amount of the compound, preferably in purified form, together with a suitable amount of carrier and/or excipient, in order to provide a form for proper administration to the patient. The composition must correspond to the route of administration.

Pharmaceutical compositions may be in the form of solutions, suspensions, emulsions, tablets, pills, capsules, powders, sustained release formulations, and the like. The pharmaceutical composition may be formulated as a suppository with standard binders and carriers such as triglycerides.

For the preparation of the pharmaceutical compositions of the present invention, pharmaceutically acceptable carriers may be either solid or liquid. Solid form compositions include powders, tablets, pills, capsules, lozenges, cachets, suppositories, and dispersible granules. The solid excipient may be one or more substances which may also act as diluents, flavors, binders, preservatives, tablet disintegrators, or microencapsulation material. In the case of powders, the excipient is preferably a finely divided solid which is in admixture with the finely divided inhibitor of the present invention. In the case of tablets, the active ingredient is mixed with a carrier having the necessary binding properties in suitable proportions and compressed into the desired shape and size. Suitable excipients are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, low melting wax, cocoa butter, and the like. To prepare suppositories, a low-melting wax, for example a mixture of fatty acid glycerides or cocoa butter, is first melted and the active ingredient is uniformly dispersed therein, such as by stirring. The molten homogeneous mixture is then poured into suitable sized molds, allowed to cool and thereby solidify. Tablets, powders, capsules, pills, cachets and lozenges can be used in solid dosage forms suitable for oral administration.

Liquid form compositions include solutions, suspensions and emulsions, for example water, saline solutions, aqueous dextrose solutions, glycerol or water/propylene glycol solutions. For parenteral injections (eg, intravenous, intra-arterial, intraosseous infusions, intramuscular, subcutaneous, intraperitoneal, intradermal, and intrathecal injections), liquid formulations may be formulated as a solution, such as an aqueous solution of polyethylene glycol. When the pharmaceutical composition is administered intravenously, saline is the preferred carrier.

Preferably the pharmaceutical composition is in unit dosage form. In this form, the composition may be divided into unit doses containing appropriate amounts of the active ingredient. The unit dosage form may be a packaged composition, wherein the package contains discrete amounts of the composition, such as packaged tablets, capsules, and powders in vials or ampoules. Also, the unit dosage form may be a capsule itself, an injection vial, a tablet, a cachet, or a lozenge, or it may be an appropriate amount of any of the above in the packaged form.

The composition, if necessary, may also contain minor amounts of lubricating or emulsifying agents, or agents that maintain the pH in a certain range.

In addition, such a pharmaceutical composition may also contain other pharmacologically active substance, such as, without limitation, adjuvants and/or additional active ingredients. Adjuvants in the context of the present invention include, without limitation, inorganic adjuvants, organic adjuvants, oil-based adjuvants, cytokines, particulate adjuvants, virosomes, bacterial adjuvants, synthetic adjuvants, or synthetic polynucleotide-based adjuvants.

According to an eighth aspect, the present invention relates to a CVP according to the fourth or sixth aspect of the present invention, a collection of nucleic acids according to the fourth or sixth aspect of the present invention, or a collection of expression vectors according to the seventh aspect of the present invention, for use in the prevention or treatment of a patient with cancer, involving cancer cells with MSI phenotype, or at risk of developing such a cancer, the cancer being preferably selected from the group consisting of colon and rectal cancer, gastric cancer, endometrial cancer, small intestine cancer, hepatobiliary tract cancer, liver cancer, neuroendocrine cancers, cervical cancer , ovarian cancer, forms of uterine sarcoma, brain cancer and skin cancer.

As indicated above, one of the specific advantages of the universal vaccine of the present invention is the fact that it provides prevention or therapy for more than one type of cancer. Accordingly, according to the eighth aspect, it is preferable to provide prevention or therapy for at least two types of cancer with MSI, more preferably at least three types of cancer with MSI. Preferred cancers are colon and rectal cancer, gastric cancer, endometrial cancer, small intestine cancer, hepatobiliary tract cancer, liver cancer, neuroendocrine cancers, cervical cancer, ovarian cancer, uterine sarcoma forms, brain cancer, and skin cancer.

Prophylaxis in the context of the present invention is preferably intended for patients who are known to be at risk of developing cancers with MSI, in accordance with clinical guidelines, including patients with gametic mutations in genes involved in the mismatched nucleotide repair (MMR) system, such like MLH-1, MSH-2, MSH-6, PMS2 and TACSTD1/EPCAM. The treatment is intended for patients with all stages of cancer (I-IV), occurring in any tissue, after MSI status has been determined in accordance with the latest clinical guidelines. The use of such a vaccine is intended for the treatment of forms of cancer with MSI status, which may be either spontaneous or pharmacologically induced.

In many cases, a single administration of CVP is insufficient to obtain the number of persistent immune cells that is required for effective protection against tumor diseases or for therapeutic treatment of tumor disease. Therefore, in order to develop long-lasting and protective immunity against said disease or to cure said disease, re-stimulation with a disease-specific biological agent is required. An administration regimen involving re-introduction of a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid is referred to herein as a "prime-boost vaccination regimen". Preferably, the prime boost vaccination schedule includes at least two administrations of a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid. The first introduction of a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid is referred to as "priming", and any subsequent introduction of the same CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such nucleic acid is called "stimulation". It will be understood from the above explanation that the same CVP may nevertheless be introduced in different arrangements with each successive introduction of a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid.

Thus, according to a preferred embodiment of the present invention, a prime boost vaccination regimen comprises one administration of a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid to prime an immune response and at least one subsequent administration to stimulate immune response. It should be understood that 2, 3, 4 or even 5 administrations to stimulate an immune response is also contemplated by the present invention.

The interval between priming and stimulation is preferably 1 week, 2 weeks, 4 weeks, 6 weeks or 8 weeks. More preferably it is 4 weeks or 8 weeks. If more than one stimulation is performed, the subsequent stimulation is preferably carried out 1 week, 2 weeks, 4 weeks, 6 weeks or 8 weeks after the previous stimulation. More preferably, the interval between any two stimulations is 4 weeks or 8 weeks.

The prime boost vaccination schedules of the present invention may be homologous or heterologous. In the case of homologous prime-boost modes, both priming and at least one stimulation are performed using the same methods of introducing a CVP, a collection of nucleic acids encoding such a CVP, or a collection of expression vectors containing such a nucleic acid. In the case of heterologous prime-boost regimens, different methods are used for priming and for stimulating the immune response. In the context of the present invention, a heterologous prime-boost scheme may include, for example, a poxvirus vector to prime an immune response and a different expression vector or CPV to stimulate an immune response.

According to one preferred embodiment of the present invention, the prime boost vaccination regimen is homologous, and according to another preferred embodiment of the present invention, the prime boost vaccination regimen is heterologous.

Accordingly, according to one aspect, the present invention relates to a collection of nucleic acids according to the fourth or sixth aspect of the present invention and/or a collection of expression vectors according to the seventh aspect of the present invention for use in the prevention or treatment of a patient with a cancer involving cancer cells with an MSI phenotype, or susceptible to the risk of developing such cancer, wherein the collection of nucleic acids and/or the collection of expression vectors is administered using a heterologous prime-boost vaccination regimen, with preferably the priming being carried out using an adenovirus vector and one or more stimulations being carried out using a poxvirus vector, preferably MVA -vector.

Examples

Example 1. Selection of mutations in the mononucleotide repeat (MNR) in the protein-coding genes of tumor samples with MSI.

Mutation Annotation Format (MAF) files based on whole exome sequencing data (publish date 4.0 October 31, 2016) and available from the TCGA information portal (https://gdc-portal.nci.nih.gov/) were analyzed for the presence of frameshift mutations readouts (FSM) in MNRs equal to or greater than 6 nucleotides located within the protein-coding segments of exomes of protein-coding genes. This analysis only considered tumors with an MSI phenotype, as defined by sample annotation data from TCGA. This group included a total of 320 tumor specimens and matched normal controls, which were 69 specimens for high-MSI colon and rectal cancer (MSI-H) (CRC), 85 specimens for gastric cancer with MSI, and 166 specimens for endometrial cancer with MSI (EC). In the second stage of selection, only FSMs were accepted in the form of a 1 nucleotide deletion, since this type of FSM is most often seen in CRC and EC tumors (1). The resulting list was further refined by accepting only those FSMs that met the following criteria: (i) the number of reads carrying the mutation was significantly higher in the tumor compared to that in a comparable normal sample (p-value by Fisher's test with FDR- correction ≤0.05); (ii) the allele frequency for FSM in a comparable normal sample was ≤25%. The remaining FSMs were further selected, leaving only those FSMs that were present in at least 5% of tumor samples in one of the three tumor types analyzed (CRC, stomach, EC). At the final selection stage, FSMs that fell into the following categories were excluded because they were considered unsuitable for the vaccine: (i) the presence of FSMs in ≥2% of samples from the collection of normal samples (EXAC database http://exac.broadinstitute.org/) and/or (ii) mRNA expression (log ₂ RSEM expression values) of the FSM-carrying gene was within the lower 20th percentile of all protein-coding genes expressed (mRNA expression data at the gene level according to TCGA), considering all three types of tumors (CRC, EC and stomach). The list thus obtained contained 1283 FSMs and was the CFSM for these tumors.

Example 2 Selection of a list of frameshift peptides with acceptable properties.

Each of the CFSM FSMs selected in Example 1 was then projected onto the corresponding mRNA sequence(s) from the NCBIREFSEQ database using ANNOVAR (Wang K, et al. (2010) NAR, 38:e164), and as wild-type mRNA (wt) and the resulting mutated mRNA were translated into proteins. Comparison of wt and mutated protein sequences provided the determination of the amino acid (aa) sequence of the corresponding frameshift (FSP) peptide. If FSM caused the formation of several FSPs with different lengths and/or aa sequences, due to the presence of several mRNA isoforms, all resulting FSPs were kept. The resulting list was selected to exclude all FSPs shorter than 4 aa that could not generate potential neoepitopes for CD8 T cells.

In addition, the amino acid sequence of FSPs to be included in the CVP was modified by adding or deleting amino acids (or FSPs that did not meet certain criteria were excluded after modification) if they met certain criteria: (i) if the FSP is shorter than 10 aa, 4 wt aa naturally occurring immediately after the FC-encoded amino acid, towards the N-terminus, is added to the N-terminus of the FSP to provide a peptide (which is referred to as mFSP because it differs from the FSP defined in the context of the present invention as the complete translation product of the protein-coding segment of a nucleic acid containing FSM, starting at the codon encoding the first amino acid that differs from the translation product of the corresponding wild-type (wt) nucleic acid without FSM), the length of which reaches the minimum length of the putative neoepitope for a CD8 T cell (8-10-mer ). Amino acid stretches common to multiple FSPs arising from the same FSM were retained only in the longest FSPs, ie. in shorter FSPs, such amino acid stretches were removed. In the final step, any segment of 8 or more contiguous amino acids also present in the human wt proteome (NCBI REFSEQ database) was removed from the FSP to minimize the risk of inducing an autoimmune response. If, after deleting the wt segment, the resulting FSP was shorter than 4 aa, it was removed from the list. The final group of FSPs thus obtained contains 1087 amino acid sequences (SEQ ID NO: 1-1087) encoded by 1059 FSMs (Table 1) and referred to as CVPs.

Example 3 Selection of the Optimal FSP Subset for an MSI Vaccine with High "Immunogenic Coverage".

SEQ ID NO: 1-1087 shows the amino acid sequence of FSP and mFSP that can be included in the CVP of the present invention. Such an exemplary CFSP contains 1087 FSPs and mFSPs. If the criteria described in Examples 1 and 2 are changed, the CFSP will include additional or fewer FSPs, and accordingly the CVP will include additional or fewer FSPs and mFSPs. Depending on the route of administration of the CVP, eg via a viral vector, in the form of naked DNA/RNA, it may not be practical or economical to produce such a large number of CVPs of FSPs and mFSPs. Thus, the present inventors have developed additional selection criteria for selecting from the CFSP prepared as described in Examples 1 and 2 an appropriate number of FSPs or mFSPs from the CFSP FSP to be included in the CVP to ensure optimal immunization of the majority of patients suffering from a particular type of cancer. with MSI, or optimal prophylaxis in patients at risk of developing cancer with MSI. Described below are such additional selection criteria that the present inventors have used to determine the subsets of FSPs and mFSPs to be included in preferred CVPs.

Previous studies in humans vaccinated with different viral antigens have shown that an antigenic sequence of approximately 400 aa contains an average of 3 immunogenic epitopes (minimum 1, maximum 12) (Figure 1) (Borthwick, N., et al. ( 2017) PLoS One 12(4), Swadling, L., et al (2014) Sci Transl Med 6(261)). Therefore, in order to obtain a good "immunogenic coverage", which in this case is meant as the expected average of 3 immunogenic epitopes, it would be desirable that FSP selected for the vaccine be presented in each patient's tumor with a total length of at least 400 aa. Thus, the present inventors have designed an algorithm for selecting FSPs from CFSPs such that the empirically established minimum immunogenic coverage threshold of 400 aa is achieved for the maximum number of MSI tumors considered. Whenever possible, the authors of the present invention aimed to achieve a target immunogenic coverage of 800 aa to compensate for the possibility that part of the FSP was not translated or presented on the surface of tumor cells. These criteria should ensure: (i) a high probability of inducing effective immune responses in a large cohort of patients; and (ii) that a large number of cancer cells in this patient will be targets of vaccine-induced immunity.

An additional constraint was included in the algorithm to achieve a maximum total length of 6000 aa for all FSPs in the final vaccine kit. This length limit reflects the preferred total number of vaccine vectors (n=4) given a maximum insert size of approximately 4500 nucleotides per vector encoding approximately ˜1500 aa contiguous FSPs and/or mFSPs.

For each of the 1059 FSMs constituting FSPs in the CVSP collection, a value of 1 or zero was assigned based on the presence or absence of FSMs, respectively, in each of 320 MSI tumor samples according to TCGA (69 for MSI-colon cancer). H, 85 for gastric cancer with MSI and 166 for endometrial cancer with MSI). For each FSM tumor type with an incidence of <5%, a value of zero was assigned to facilitate the inclusion in the vaccine of those FSMs that are more prevalent in each tumor group.

As a first step, the algorithm ranked 1059 FSMs according to a score that is the product of the total length of all FSPs resulting from each FSM and the frequency of FSMs observed in 320 tumors with MSI (length in aa x number of samples containing FSMs/total number of samples).

The first FSM listed for the vaccine was the one with the highest score. Further, the algorithm worked cyclically, selecting in each round the FSM that provided for the maximum number of tumors an increase in their immunogenic coverage. If more than one FSM met this condition, the FSM with the highest score was selected. If an FSM was selected, all FSPs encoded by the nucleic acid with that FSM were added to the vaccine inventory, and the immunogenic coverage of the samples carrying the selected FSM was updated accordingly by adding the total length in aa of the corresponding FSPs. Once the target immunogenic coverage threshold was reached for a sample (in this case chosen to be 800 aa for colon and rectal cancer and gastric cancer, 400 aa for endometrial cancer), the algorithm reset it for selection of subsequent FSMs, while considering only those samples for which the target coverage has not yet been reached.

The algorithm continued to add FSP or mFSP to the list for the vaccine, ie. amino acid sequences that form CVPs until one of the following three conditions is met: (i) the total length in aa of all selected FSPs encoded by nucleic acids with selected FSMs reaches 6000 aa; (ii) all cancer samples had an immunogenic coverage ≥ target threshold, or (iii) there were no more FSMs that increased the immunogenic coverage of the samples below the threshold.

The final subset of FSPs selected using the described algorithm contains 209 FSPs resulting from 204 FSMs, with a total length of 6021 aa, and is named Nous-209 (SEQ ID NO: 1-209). This FSP subset provides >400 aa immunogenic coverage for 98% of colon and rectal tumor specimens according to TCGA with a median of 50 FSPs per sample and a median immunogenic coverage of 1322 aa. Similarly, the FSPs in Nous-209 provide immunogenic coverage of ≥400 aa in 95% of gastric tumor samples with a median of 46 FSPs per sample and a median coverage of 1178 aa. Finally, immunogenic coverage of >400 aa is achieved in 70% of endometrial tumor samples according to TCGA with a median of 21 FSPs per sample and a median coverage of 512 aa. Immunogenic coverage remains very high for colon and rectal cancer and gastric cancer (93% and 83% respectively) with a target value of 800 aa.

Example 4 Validation of Nous-209 as a high immunogenic coverage MSI cancer vaccine candidate.

As a first step in checking the list of FSPs and mFSPs included in Nous-209, the present inventors performed next generation sequencing (NGS) using a panel of cell lines with MSI (7 CRC and 1 EC). At the genome level (exome sequencing), the number of FSMs found in each cell line, which were also included in Nous-209, was such that all cell lines significantly passed the minimum immunogenic coverage threshold of 400 aa, with a mean coverage (total FSP length) 2037 aa (Fig. 2A).

Secondly, the present inventors analyzed these same cell lines by RNA sequencing to determine how many FSMs in Nous-209 that were found by exome sequencing were also present at the transcriptional level. Of note, immunogenic coverage above 400 aa was also maintained for all cell lines at the RNA sequencing level (FIG. 2B).

Results similar to those obtained for MSI cell lines were obtained by exome sequencing of six fresh frozen comparable MSI colon and rectal tumor biopsies (tumor and normal tissue). For all patients analyzed, immunogenic coverage was above the minimum threshold of 400 aa, with a mean coverage of 926 aa. Four of the six samples exceeded the target threshold of 800 aa (FIG. 3).

Moreover, to assess the immunogenicity potential of Nous-209, the present inventors calculated how many of the vaccine-encoded FSPs in MSI CRC biopsies were predicted to produce epitopes with the proper binding profile (IC ₅₀ ≤ 500 nM) for MHC-I molecules. To this end, the present inventors first deduced the HLA haplotype for 6 patients from sequencing data from their biopsy specimens. The present inventors then performed HLA-I patient-specific binding predictions for the subset of vaccine-encoded FSPs present in each patient using the IEDB software (http://www.iedb.org/). Each patient with MSI had an average of 67 epitopes (min. 29, max. 141) predicted to bind to their own HLA-I haplotype (FIG. 4).

Taken together, these results demonstrate that high immunogenic coverage can be achieved in a subset of FSPs, such as the subset included in Nous-209, in both MSI cell lines and primary tumor biopsies.

The present inventors then tested whether immune responses against FSP in Nous-209 could be measured in vivo. To this end, the authors of the present invention immunized HLA-A02+ transgenic mice using a subset of FSP Nous-209. Vaccine-induced immune responses were assessed by cytofluorimetry (FACS) using nonamers selected from these FSPs based on HLA-A02 binding prediction. Figure 5 shows representative CD8 T cell responses to HLA-A02 nonamer derived from FSP corresponding to SEQ ID NO: 123 in 5 animals as measured by intracellular staining (ICS) for interferon y (IFN-y) (panel A). A graph of the results of FACS using the gating strategy for IFN-y+CD8 T cells from one of these mice is presented in panel B, which shows a significant percentage (5.6%) of FSP-reactive T cells.

Example 5 Construction of nucleic acid cassettes for Nous-209.

The FSPs in Nous-209 were divided into 4 subsets to produce artificial genes approximately 4500 nucleotides in length, each encoding approximately 1500 aa, suitable for cloning into the following viral backbones: Hominid adenovirus (GAd) and modified vaccinia virus Ankara (MVA). Each gene was assembled by joining the FSP sequences one by one without any linker. The 209 FSPs were distributed across 4 genes such that each gene encoded approximately the same number of FSPs with similar scores according to the algorithm described in Example 3 and encoded FSPs in which the corresponding FSMs had a similar overall distribution of observed frequencies across the three cancer types. This procedure resulted in:

Gene 1=1507 aa (46 FSP)

Gene 2=1501 aa (54 FSP)

Gene 3=1506 aa (59 FSP)

Gene 4=1507 aa (51 FSP).

Each artificial gene was assembled in four different arrangements (A, B, C and D) corresponding to a different FSP order (Table 2). The genes in layouts A and C were used to construct GAd vectors, while the genes in layouts B and D were cloned into MVA backbones, following a heterologous prime-boost vaccination strategy. FSP shuffling between layouts A and B and between layouts C and D was performed to avoid encoding both GAd and MVA the same border aa sequences between adjacent FSPs, which would stimulate immune responses to the linked epitopes. To select the FSP order for each gene in the two arrangements, the present inventors used a procedure that yields 50,000 different polypeptides in which the FSPs are randomly linked. Of these, the present inventors selected two with different border sequences and a disordered sequence in the first 50 aa (mean disorder tendency >0.50 as assessed by the IUPRED software (Dosztanyi Z., et al. (2005) Bioinformatics 21, 3433) (Fig. 6). The latter choice was based on the fact that the disordered N-terminus facilitates processing by the proteasome, and therefore should increase the likelihood of presentation of FSP-derived epitopes on MHC-I molecules. The artificial polypeptides were then screened w silico with respect to similarity to human reference proteins to avoid the formation of cross-reactive regions with the human proteome The Aa sequences of the four poly-FSP chains in layouts A, B, C and D are shown as SEQ ID NOs: 1088-1091 (layout A), SEQ ID NO: 1092-1095 (layout B), SEQ ID NO: 1155-1158 (layout C) and SEQ ID NO: 1159-1162 (layout D), corresponding to the times FSP order (Table 2). The genes in layouts A and C were used to construct GAd vectors, while the genes in layouts B and D were cloned into MVA backbones, following a heterologous prime-boost vaccination strategy. The FSPs were permuted respectively between layouts A and B and between layouts C and D. Their respective nt sequences are SEQ ID NO: 1096-1099 (layout A), SEQ ID NO: 1100-1103 (layout B), SEQ ID NO: 1163-1166 (layout C) and SEQ ID NO: 1167-1170 (layout D).

To construct an expression cassette, a sorting signal (either human tissue plasminogen activator signal peptide (hTPA; SEQ ID NO: 1104) or human invariant chain (hINV; SEQ ID NO: 1105) was added to the N-terminus, and the influenza virus HA tag sequence (SEQ ID NO: 1106) was added to the C-terminus of each polypeptide sequence shown in Figure 7. The extended sequence of the thus constructed polypeptides is shown as SEQ ID NO: 1107-1110 (layout A), SEQ ID NO: 1111-1114 (layout B), SEQ ID NO: 1171-1174 (layout C) and SEQ ID NO: 1179-1182 (layout D) for hTPA and SEQ ID NO: 1115-1118 (layout A), SEQ ID NO: 1119-1122 (layout B), SEQ ID NO: 1175-1178 (layout C) and SEQ ID NO: 1183-1186 (layout D) for hINV Corresponding nt sequences to obtain the indicated poly-BvR chains were codon-optimized based on the codon usage of y human (SEQ ID NO: 1123-1126 for layout A, SEQ ID NO: 1127-1130 for layout B, SEQ ID NO: 1187-1190 for layout C and SEQ ID NO: 1191-1194 for layout D with hTPA; SEQ ID NO: 1131-1134 for layout A, SEQ ID NO: 1135-1138 for layout B, SEQ ID NO: 1195-1198 for layout C, and SEQ ID NO: 1199-1202 for layout D with hINV). In addition, the Kozak sequence (CGCGACTTCGCCGCC) was placed directly upstream of the start codon to ensure efficient translation initiation, and the TAA stop codon was placed below the HA tag. Finally, two flanking segments containing unique restriction sites were added to the 5' and 3' ends of the nucleotide sequence, respectively, to facilitate subcloning of the cassettes (FIG. 7). Nt sequences representing artificial genes (SEQ ID NO: 1139-1142 for layout A, SEQ ID NO: 1143-1146 for layout B, SEQ ID NO: 1203-1206 for layout C, and SEQ ID NO: 1207-1210 for layout D with hTPA, SEQ ID NO: 1147-1150 for layout A, SEQ ID NO: 1151-1154 for layout B, SEQ ID NO: 1211-1214 for layout C, and SEQ ID NO: 1215-1218 for layout D with hINV) , was prepared using standard oligonucleotide synthesis methods and subcloned between the TetO-CMV promoter (hCMV promoter with binding site for Tet-penpeccopa) and BGH polyA (bovine growth hormone).

Example 6 Confirmation of immunogenicity.

Immunogenicity of 4 GAd vectors (GAd20-209-FSP) containing 4 genes in layout A (polypeptide sequence SEQ ID NO: 1107-1110, nucleotide sequence SEQ ID NO: 1139-1142) and 4 MVA vectors (MVA-209 -FSP) containing 4 genes in layout B (polypeptide sequence SEQ ID NO: 1111-1114, nucleotide sequence SEQ ID NO: 1143-1146) were evaluated in mice (CB6F1 mouse strain). GAd20-209-FSP were constructed based on GAd20 (SEQ ID NO: 1219) from which the E3 region was removed and the E1 region was replaced with genes. Mice were immunized by a single intramuscular immunization with GAd20-209-FSP at doses of 10 18 viral particles (vp) for each vector and challenged with MVA-209-FSP at doses of 1017 plaque-forming units (pfu) two weeks later. The induction of T cell responses to vaccine-encoded FSPs was measured by ELIspot analysis using synthetic peptides spanning the 209 FSP sequences. Synthetic peptides were diluted in DMSO and mixed to obtain 16 pools. Immune responses (interferon gamma (IFN-y) producing T cells per million splenocytes) were analyzed 2 weeks after priming and one week after challenge. The data demonstrate the induction of a T-cell mediated immune response after GAd20-209-FSP priming and the potent stimulation of the immune response obtained after administration of MVA-209-FSP (FIG. 8). Responses were directed to multiple FSPs distributed across vaccine constructs, as evidenced by their detection for each of the 16 peptide pools (FIG. 9). It is important to note that the co-administration of the vectors did not affect the immune responses against FSP encoded by one vector, which ruled out immune interference in the presence of the FSP vaccine mixture (Fig. 10). Finally, the quality of T cell responses was assessed by intracellular staining (ICS) using two pools of peptides (pool 1 and pool 3), and demonstration of the induction of FSP-specific CD4 and CD8 IFN-gamma + T cells (FIG. 11).

As explained in Example 4, it was tested whether immune responses against FSP in Nous-209 could also be measured in vivo. HLA-A02+ transgenic mice were immunized with a subset (30 FSP) of Nous-209 FSP or DMSO as a negative control. Vaccine-induced immune responses were assessed by cytofluorimetry (FACS) using nonamers selected from 30 FSPs based on HLA-A02 binding prediction. In FIG. 11 shows a representative response of CD8 T cells in 5 animals to the HLA-A02 nonamer present in FSP SEQ ID NO: 123 as measured by intracellular staining (ICS) for interferon (IFN-y) (panel A). A graph of the results of FACS using the gating strategy for IFN-γ+CD8 T cells from one of these mice is presented in panel B, which shows a significant percentage (5.6%) of FSP-reactive T cells.

Taken together, these results demonstrate that

i) the constructs are highly immunogenic and induce a CD4- and CD8-mediated immune response, ii) The immune response is directed to 16 different pools of peptides and therefore there is recognition of at least 16 different epitopes (one for each pool). Even in inbred mice (virtually genetically identical mice), the at least one epitope per 400 aa rule has been shown to hold true, since the presence of 16 epitopes per 6000 aa vaccine corresponds, on average, to at least one immunogenic epitope per 375 aa.

iii) No apparent interference effect, in particular no suppression between the individual vectors, as comparable immune responses to vector 1 encoded antigens were measured whether vector 1 was used alone or in combination with the other three vectors. Similarly, comparable immune responses to vector 2 encoded antigens were measured whether vector 2 was used alone or in combination with the other three vectors.

--->

SEQUENCE LIST

<110> NOWCOME AG

<120> UNIVERSAL VACCINE BASED ON GENERAL TUMOR NEOANTIGENS FOR

PREVENTION AND TREATMENT OF CANCER WITH MICROSATELLITE INSTABILITY (MSI)

<130> 854-11 PCT

<150> 17181026.0

<151> 2017-07-12

<160> 1219

<170>PatentIn version 3.5

<210> 1

<211> 154

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1

Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys Val Pro Val Ala Glu

1 5 10 15

Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala Ala Gly Thr Arg Cys

20 25 30

Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro Ala Ser Ser Pro Thr

35 40 45

Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg Arg Lys Arg Ser Ser

50 55 60

Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu Arg Met Met Leu Pro

65 70 75 80

Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro

85 90 95

Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg

100 105 110

Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu

115 120 125

Gly Leu Arg Pro Pro His Leu Pro Ser Cys His Pro Leu Pro Gln Leu

130 135 140

Leu Gly Pro Pro Pro His Gln Ala Pro

145 150

<210> 2

<211> 74

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 2

Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr

1 5 10 15

Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro His

20 25 30

Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser His His

35 40 45

Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly

50 55 60

Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu

65 70

<210> 3

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 3

Gly Gly Lys Lys Arg Ser Lys Phe

15

<210> 4

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 4

Val His Lys Lys Arg Gly Leu Phe

15

<210> 5

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 5

Val Pro Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys

1 5 10 15

Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile

20 25 30

Leu Gly Pro Gln Arg His Thr Pro

35 40

<210> 6

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 6

Gln Pro Ser Leu Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu Ser

1 5 10 15

Gln Pro Pro Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala Thr Leu

20 25 30

Arg Cys Pro Ala Val Pro Ala Ala Ala Cys Pro Pro

35 40

<210> 7

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 7

Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala

1 5 10 15

Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser

20 25 30

<210> 8

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 8

Arg Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg

1 5 10 15

Lys Tyr Leu Arg Val

20

<210> 9

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 9

Val Leu Lys Lys Asn Arg Lys Gln

15

<210> 10

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 10

Thr Ala Phe Phe Leu Leu Asp Ile Ile Leu

1 5 10

<210> 11

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 11

Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg Lys Lys

1 5 10

<210> 12

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 12

Arg Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro Ala Asn

1 5 10 15

Gln Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg Thr Glu

20 25 30

<210> 13

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 13

Cys Arg Cys Arg Arg Glu Tyr Arg Val Thr Met

1 5 10

<210> 14

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 14

Gly Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser Asp Thr Thr Tyr

1 5 10 15

Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His

20 25 30

Leu Lys Thr Thr Ser Leu

35

<210> 15

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 15

Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile His His

1 5 10 15

Arg Lys Asn Pro Leu Leu Pro Met Arg

20 25

<210> 16

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 16

Thr Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg

1 5 10 15

Gly Gly Thr Thr Ile Gly Glu Val Thr Ser

20 25

5

<210> 17

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 17

Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser

1 5 10 15

Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln

20 25

<210> 18

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 18

Met Pro Ser Ser Trp Ser Ser Ser Gly Arg Thr

1 5 10

<210> 19

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 19

Pro Gly Thr Ser Leu Gln Cys Tyr Leu Asn

1 5 10

<210> 20

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 20

Lys Gly Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn

1 5 10 15

Gln Gly His Leu Leu Met Ile Leu Leu His Asn

20 25

<210> 21

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 21

Asn Ile Leu Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln

1 5 10 15

Glu

<210> 22

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 22

Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr

1 5 10

<210> 23

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 23

Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr

1 5 10 15

Gln

<210> 24

<211> 73

<212> PROTEIN

<213> Homo sapiens

<400> 24

Phe Leu Ile His Leu Arg Lys Val Asp Thr His His Leu Gln Val Val

1 5 10 15

Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg

20 25 30

Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala

35 40 45

Ala Gln Ala Pro Pro Lys Ser His Phe Pro Pro His Pro Ser Gly Pro

50 55 60

Ser Arg Thr Ser Ser Leu Gln Leu Arg

65 70

<210> 25

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 25

Glu Trp Gly Gly Gln Ala His Arg Gln Arg Asn

1 5 10

<210> 26

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 26

Ile Val Phe Phe Leu Ala Pro Tyr Phe Pro

1 5 10

<210> 27

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 27

Pro Lys Phe Phe Leu Thr Phe Ser Gln

15

<210> 28

<211> 77

<212> PROTEIN

<213> Homo sapiens

<400> 28

Ala Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro Thr Thr Arg Leu

1 5 10 15

Met Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu Leu Thr Gln Pro

20 25 30

Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu His Pro Gln Trp

35 40 45

Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys

50 55 60

Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr Trp

65 70 75

<210> 29

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 29

Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys Cys

1 5 10

<210> 30

<211> 38

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 30

Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg Gln Asn His

1 5 10 15

Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu

20 25 30

Arg Ser Pro Trp Ile Phe

35

<210> 31

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 31

Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro

1 5 10 15

Arg Ser

<210> 32

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 32

Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu Ser

1 5 10 15

<210> 33

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 33

Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly Val Arg

1 5 10 15

Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg

20 25 30

<210> 34

<211> 53

<212> PROTEIN

<213> Homo sapiens

<400> 34

Ala Leu Arg Ser Met Pro Lys Ser Arg Asn Gln Val Leu Phe Arg Arg

1 5 10 15

Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu

20 25 30

Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser

35 40 45

Gln Ser Met Leu Val

50

<210> 35

<211> 34

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 35

Leu Gln Ala His Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp Trp

1 5 10 15

9

Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln Gln Ser

20 25 30

Leu Thr

<210> 36

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 36

Cys Thr Asn Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys

1 5 10 15

Arg Val Phe Ile Leu Leu Leu Ser Asp Lys Lys

20 25

<210> 37

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 37

Pro Leu Phe Phe His Gly Asp Leu Cys

15

<210> 38

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 38

Val Ser Lys Lys Arg Leu Pro Lys Leu

15

<210> 39

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 39

Arg Arg Lys Lys Arg Glu Asp His Cys Gly Leu

1 5 10

<210> 40

<211> 17

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 40

Cys Leu Arg Val Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg

1 5 10 15

gly

10

<210> 41

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 41

Arg Glu Ser Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro

1 5 10 15

<210> 42

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 42

Ala Ser Ser Pro Gly His Pro Leu Gly Pro

1 5 10

<210> 43

<211> 56

<212> PROTEIN

<213> Homo sapiens

<400> 43

Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly

1 5 10 15

Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser Leu

20 25 30

Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg Val

35 40 45

Leu Trp Met Pro Thr Ser Thr Val

50 55

<210> 44

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 44

Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu

1 5 10 15

Phe Pro Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys

20 25 30

<210> 45

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 45

Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe

1 5 10

eleven

<210> 46

<211> 39

<212> PROTEIN

<213> Homo sapiens

<400> 46

Asn Gly Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys Ile Ser

1 5 10 15

Val Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile Thr Glu

20 25 30

Cys Val Arg Thr Ala Leu Asn

35

<210> 47

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 47

Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn Leu

1 5 10

<210> 48

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 48

His Gln Met Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe Trp

1 5 10 15

Asp Met

<210> 49

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 49

Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys

1 5 10

<210> 50

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 50

Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu Lys Met Glu Ile Gln

1 5 10 15

Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu Cys

20 25

<210> 51

<211> 43

12

<212> PROTEIN

<213> Homo sapiens

<400> 51

Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro Leu Arg Asn

1 5 10 15

Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser

20 25 30

Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met

35 40

<210> 52

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 52

Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser Cys Pro Phe Gln Ser Pro

1 5 10 15

Thr Lys Arg Leu

20

<210> 53

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 53

Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr

1 5 10 15

Lys Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val

20 25 30

Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln

35 40 45

Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg

50 55 60

<210> 54

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 54

Asn Ala Lys Lys Met Pro Phe Gln

15

<210> 55

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 55

13

Ala Asp Lys Lys Asn Val Arg Ser

15

<210> 56

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 56

Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser Cys

1 5 10

<210> 57

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 57

Ile Gln Lys Lys Lys Gln Lys Ser

15

<210> 58

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 58

Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser

1 5 10 15

Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro

20 25 30

Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala

35 40

<210> 59

<211> 46

<212> PROTEIN

<213> Homo sapiens

<400> 59

His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr Cys Ile

1 5 10 15

Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp

20 25 30

Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr

35 40 45

<210> 60

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 60

Arg Lys Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro

1 5 10

14

<210> 61

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 61

Cys Gln Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu

1 5 10

<210> 62

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 62

Arg Leu Pro Cys Lys Val Thr Glu Ser Ser

1 5 10

<210> 63

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 63

Asn Gln Gln Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser

1 5 10 15

<210> 64

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 64

Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu Thr Lys

1 5 10 15

Arg Glu Gly Pro Glu Thr

20

<210> 65

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 65

Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg

1 5 10 15

Ala Val Arg Arg Lys Gly

20

<210> 66

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 66

Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser Pro Ser

15

1 5 10

<210> 67

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 67

Gln Arg Pro Pro Leu Gln Pro Val

15

<210> 68

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 68

Leu Lys Lys Lys Tyr Leu Val Gln

15

<210> 69

<211> 50

<212> PROTEIN

<213> Homo sapiens

<400> 69

Thr Ser Arg Ile Leu Asn Leu Gln Val Leu Lys Lys Ile Leu Arg Ser

1 5 10 15

Phe Met Lys Leu Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg Thr

20 25 30

Lys Leu Glu Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser

35 40 45

Tyr Lys

50

<210> 70

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 70

Ser Thr Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly

1 5 10 15

Pro Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys

20 25 30

Leu Arg Leu Arg Asn Thr Ser Pro

35 40

<210> 71

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 71

16

Asn Ser Arg Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr

1 5 10 15

<210> 72

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 72

Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu

1 5 10

<210> 73

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 73

Asp Lys Lys Lys Lys Ser Thr Glu Lys Thr Lys

1 5 10

<210> 74

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 74

Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys

1 5 10 15

Cys Leu Pro Gly Leu Thr His Pro Ala His Gln Pro Leu Gly Ser Met

20 25 30

<210> 75

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 75

Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg

1 5 10

<210> 76

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 76

His Leu Lys Lys Thr Ser Ala Lys

15

<210> 77

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 77

Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala Pro Gln

1 5 10 15

17

<210> 78

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 78

Gly Gln Ala Phe Gly Ser Leu Cys Gln Leu Val Ser Ala Asp Phe Ser

1 5 10 15

Ala Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His Leu Leu Ile

20 25 30

<210> 79

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 79

Gly His Arg Glu Gly Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser

1 5 10 15

Phe Pro Pro

<210> 80

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 80

Ser Glu Lys Lys Arg Lys Leu Pro

15

<210> 81

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 81

Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val Ala Met

1 5 10 15

Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu Ser Thr Leu

20 25 30

Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro Val Thr Pro Leu

35 40 45

Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro Gln Thr Ala

50 55 60

<210> 82

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 82

18

Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu

1 5 10 15

Pro Ser Ser Asp Leu Ala Val Leu Ile Ser Ser Ser Thr Ser His Phe

20 25 30

Leu Met Arg Ser Pro Val Leu Pro Ser Ser Arg Leu Thr Cys Ala Ser

35 40 45

Pro Gln Leu Pro Arg Met Trp Thr Trp Ser Ser Ser Trp Leu Lys

50 55 60

<210> 83

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 83

Trp Leu Lys Asn Trp Lys Arg Phe Lys Leu Lys Lys Lys Tyr

1 5 10

<210> 84

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 84

Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu

1 5 10

<210> 85

<211> 155

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 85

Tyr Pro Arg Leu Lys Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys

1 5 10 15

Leu Ala Gly Ser Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg Gln

20 25 30

Glu Cys Gly Arg Arg Ala Ser Val Pro Cys Arg Pro Met Ile Gly Ser

35 40 45

Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser Ala Met Gly

50 55 60

Val Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr

65 70 75 80

Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met Met Ser Trp Met Pro

85 90 95

Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu

19

100 105 110

Cys Pro Ala His Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr

115 120 125

Pro Leu Ser Ala His Pro Val Pro Val Leu Ser Gly Cys His Leu Ala

130 135 140

Val Arg Thr Ser Met Leu Thr Leu Leu Pro Met

145 150 155

<210> 86

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 86

Val Leu Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly

1 5 10 15

<210> 87

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 87

Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile

1 5 10

<210> 88

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 88

Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe

1 5 10

<210> 89

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 89

Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln Thr Tyr

1 5 10 15

Phe Gly Ser

<210> 90

<211> 50

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 90

20

Gly Lys Gly Leu Thr His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn

1 5 10 15

Lys Arg Asn Lys Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile

20 25 30

Lys Arg Lys Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro Glu

35 40 45

Ser Asn

50

<210> 91

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 91

Thr Pro Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro

1 5 10

<210> 92

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 92

Met Ser Arg Thr Pro Pro Arg Arg Val Cys

1 5 10

<210> 93

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 93

Leu Ala Lys Lys Thr Leu Thr Asn

15

<210> 94

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 94

Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys Ile

1 5 10 15

Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val His Val Leu Met Phe

20 25 30

<210> 95

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 95

Val Ser Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu

1 5 10 15

21

Asn Trp Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met

20 25 30

<210> 96

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 96

Ile Ile Cys Ile Lys Asn Met Phe Leu Gln Arg Lys Gly Phe Leu Asn

1 5 10 15

Leu Ser His Val Glu Lys Phe

20

<210> 97

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 97

Leu Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile Ser

1 5 10

<210> 98

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 98

Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln

1 5 10

<210> 99

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 99

Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe Phe Thr

1 5 10 15

Ser Ala His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln

20 25 30

<210> 100

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 100

Asn Pro Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys

1 5 10 15

Pro Ser Gln Lys Asn His Leu Tyr

20

22

<210> 101

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 101

Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn

1 5 10

<210> 102

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 102

Phe Asp Lys Lys Gly Phe Val Ile Leu Ser

1 5 10

<210> 103

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 103

Gly Ser Pro Pro Leu His Leu Cys Gln Pro Leu Arg

1 5 10

<210> 104

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 104

Glu Leu Lys Lys Asn Leu Leu Leu Pro

15

<210> 105

<211> 77

<212> PROTEIN

<213> Homo sapiens

<400> 105

Arg His Pro His Arg Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu

1 5 10 15

Ala Phe Pro Leu Pro Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His

20 25 30

Ser Ser Val Leu Ala Thr Gly Leu His Leu His Arg Gln Ile Ser Leu

35 40 45

Pro Leu Arg Gly Phe Leu Leu His Gln Pro Leu Pro Gly Gln His Leu

50 55 60

Trp Leu Ser His Arg Leu Arg Leu Arg Leu Pro Arg Thr

65 70 75

<210> 106

<211> 13

23

<212> PROTEIN

<213> Homo sapiens

<400> 106

Ala Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp

1 5 10

<210> 107

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 107

Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His

1 5 10

<210> 108

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 108

Ile Thr His Pro Phe Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn

1 5 10 15

Asn Thr Gln Ile Arg Leu Leu Lys Pro

20 25

<210> 109

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 109

Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr Ala

1 5 10 15

Asn Met Cys Trp

20

<210> 110

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 110

Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe Arg Asn Tyr

1 5 10 15

Pro Arg

<210> 111

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 111

Ala Phe Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr

24

1 5 10 15

Gly Cys Trp

<210> 112

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 112

Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser

1 5 10

<210> 113

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 113

Met Ala Lys Lys Ser Gln Gln Pro Leu

15

<210> 114

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 114

Glu Ala Lys Lys Ser Met Lys Arg Ser

15

<210> 115

<211> 125

<212> PROTEIN

<213> Homo sapiens

<400> 115

Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln

1 5 10 15

Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala

20 25 30

Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe

35 40 45

Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg

50 55 60

Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg

65 70 75 80

Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly

85 90 95

Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe

100 105 110

25

Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr

115 120 125

<210> 116

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 116

Ala Ser Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr

1 5 10 15

ile

<210> 117

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 117

Ala Ser Pro Gln Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met

1 5 10 15

Lys Arg Arg Met Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu

20 25 30

Leu Pro Gly

35

<210> 118

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 118

Gly Asp Cys Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro

1 5 10 15

Ala Ser Gly Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala

20 25 30

Cys Arg Ala Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala

35 40 45

<210> 119

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 119

Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly

1 5 10

<210> 120

<211> 10

<212> PROTEIN

26

<213> Homo sapiens

<400> 120

Met Leu Asn Gln Lys Cys Leu Ala Pro Phe

1 5 10

<210> 121

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 121

Ser Leu Ile Leu Ser Phe Gln Arg Val Lys

1 5 10

<210> 122

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 122

Gly Ala Lys Lys Ile Thr Phe Leu

15

<210> 123

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 123

Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys

1 5 10 15

Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly

20 25 30

Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala

35 40

<210> 124

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 124

Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln Cys

1 5 10 15

<210> 125

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 125

Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys

1 5 10

<210> 126

<211> 12

27

<212> PROTEIN

<213> Homo sapiens

<400> 126

Pro Ile Pro Pro His Leu Arg His Pro Leu Leu Leu

1 5 10

<210> 127

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 127

Val Pro Met Ser Leu Ala Cys Ser Ile Thr Val Ser Phe Val Gly Gln

1 5 10 15

Ile Ser Thr Leu Val Leu Asp Val Ser Ser Ile Ser His Trp

20 25 30

<210> 128

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 128

Asp Ser Lys Lys Met Thr Gln Lys

15

<210> 129

<211> 64

<212> PROTEIN

<213> Homo sapiens

<400> 129

Arg Ser Leu Leu Tyr Ser Arg Met Cys Leu Val Gln Ile Pro Thr His

1 5 10 15

Thr Trp Glu Pro Asp Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp

20 25 30

Ala Thr Gln Ser Phe Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala

35 40 45

Ile Pro Cys Cys Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser

50 55 60

<210> 130

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 130

Arg Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln Leu Ala Ile Gln

1 5 10 15

Val Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe Gly

20 25

28

<210> 131

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 131

Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly

1 5 10

<210> 132

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 132

Ile Leu Ala Met Thr Leu Thr Leu Asn Gly

1 5 10

<210> 133

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 133

Gln Arg Pro Pro Arg Ser Ile Leu Ser Leu

1 5 10

<210> 134

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 134

Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr

1 5 10

<210> 135

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 135

Gly Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr

1 5 10 15

Thr Pro Ser Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln

20 25 30

Ser Lys Lys Thr Met Thr Ala Ala Gly Leu Thr Pro

35 40

<210> 136

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 136

Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile Ser

1 5 10 15

29

Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu Ala

20 25

<210> 137

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 137

Ala Phe Ser Gly Arg Ile Leu His Gln Pro Glu Met Ala Ala Met Arg

1 5 10 15

Leu Met Pro Lys Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr

20 25 30

<210> 138

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 138

Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln Val Met Leu

1 5 10 15

Cys

<210> 139

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 139

Gln Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys

1 5 10 15

Gln Ser Gln Arg Arg Gly Lys Leu Ser

20 25

<210> 140

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 140

Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro Gly

1 5 10 15

His Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp Ser Ser Lys

20 25 30

<210> 141

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 141

Asn Lys Val Arg Gln Asn Phe Lys Arg Asn

thirty

1 5 10

<210> 142

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 142

Ile Tyr Pro Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly

1 5 10

<210> 143

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 143

Val Asp Pro Pro Gly Ser Val Trp Ile Leu Pro Cys

1 5 10

<210> 144

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 144

Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val His

1 5 10

<210> 145

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 145

Lys Gly Lys Lys Arg Arg Lys Glu Ile

15

<210> 146

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 146

Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe

1 5 10

<210> 147

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 147

Arg Gly Pro Pro Leu His Thr Arg

15

<210> 148

<211> 170

<212> PROTEIN

<213> Artificial sequence

31

<220>

<223> Modified FSP Homo sapiens

<400> 148

Asn Glu Gly Asn Asn His Trp Pro Arg Val Glu Met Pro Thr Gly Trp

1 5 10 15

Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu

20 25 30

Gln Gln His Leu Lys His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys

35 40 45

Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe

50 55 60

Lys Thr Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro

65 70 75 80

Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr

85 90 95

Thr Gly Val Tyr Gln His Val Trp Leu Ile Cys Phe Val Phe Phe Val

100 105 110

Cys Val Glu Thr Gly Phe Cys His Val Ala Gln Val Gly Leu Glu Arg

115 120 125

Leu Val Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro

130 135 140

Lys Pro Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu

145 150 155 160

Arg Val Gly Lys Gly Val Trp Ala Arg Leu

165 170

<210> 149

<211> 78

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 149

Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met Met Met Met

1 5 10 15

Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met Thr Met Met

20 25 30

Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro Arg Thr

35 40 45

Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln Arg Arg

32

50 55 60

Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp Gly

65 70 75

<210> 150

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 150

Arg Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu Ile Ala

1 5 10 15

His Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro Arg Leu

20 25 30

Leu Thr Arg Lys Lys Pro Gly Lys

35 40

<210> 151

<211> 15

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 151

Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg Met Trp Trp

1 5 10 15

<210> 152

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 152

Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser Arg Arg Gly

1 5 10 15

Met Lys Ser Ser Val Arg Leu Lys Asn

20 25

<210> 153

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 153

Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe Phe Arg Gly Ser

1 5 10 15

Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys

20 25

<210> 154

<211> 30

<212> PROTEIN

33

<213> Homo sapiens

<400> 154

Trp Leu Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln

1 5 10 15

Trp Gly Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly

20 25 30

<210> 155

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 155

Glu Glu Lys Lys Asn Gly Arg Ile Ser Ser

1 5 10

<210> 156

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 156

Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg Leu

1 5 10

<210> 157

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 157

Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met Leu Cys Leu Pro

1 5 10 15

<210> 158

<211> 114

<212> PROTEIN

<213> Homo sapiens

<400> 158

Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser Asn Gln Leu Gln Thr

1 5 10 15

Val Gln Met Leu Cys Leu Pro Pro Leu Lys Gln Gly Glu Arg Ile Ile

20 25 30

Trp Pro Leu Gly Gly Phe Gln Ile Pro Asn Phe Phe Trp Ser Leu Gly

35 40 45

Ile Gly His Thr Gly Ala Trp Trp His Thr Gly Arg Arg Arg Arg Glu

50 55 60

Trp Gly Gly Ser Thr Val Ser Arg Ser Pro Leu Trp Ile Ser Ser Met

65 70 75 80

Ile Tyr Leu Arg Gly Met Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr

34

85 90 95

Thr Arg Val Ser Trp Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala

100 105 110

Ser Ser

<210> 159

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 159

Ile Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile

1 5 10

<210> 160

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 160

Ser Arg Arg Asn Arg Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met

1 5 10 15

Ser Leu Gln Ala

20

<210> 161

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 161

Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp

1 5 10 15

<210> 162

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 162

Phe Leu Pro Ser Arg Phe Pro Trp Gly Pro Pro Pro Leu

1 5 10

<210> 163

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 163

Asn Ser Val Ile Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu

1 5 10 15

Ser Ser Tyr Asn Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala

20 25 30

35

Ile Glu Phe Ala Val Leu Lys Leu Leu Asn Met Asp Leu

35 40 45

<210> 164

<211> 61

<212> PROTEIN

<213> Homo sapiens

<400> 164

Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser Pro Gly Pro

1 5 10 15

Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala Ile Gly Lys Leu

20 25 30

Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala Arg Glu

35 40 45

Ala Gly Arg Phe Ala Val Leu Gly Thr Met Val Met Lys

50 55 60

<210> 165

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 165

Ser Pro Leu Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys

1 5 10 15

Leu Trp Glu Ile Pro Ser Val Thr Leu Leu Ala

20 25

<210> 166

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 166

Thr Leu Ala Ile Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Ser Thr

1 5 10 15

Val Thr Ala Ala Pro Ser Gly Gln Tyr Phe

20 25

<210> 167

<211> 46

<212> PROTEIN

<213> Homo sapiens

<400> 167

Leu Leu Leu Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys

1 5 10 15

Gly Glu Cys Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile

20 25 30

36

Leu Lys Gln Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met

35 40 45

<210> 168

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 168

Ser Leu Phe Phe Ser His Cys Leu Cys

15

<210> 169

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 169

Leu Lys Lys Lys Val Asn Ala Cys Ser

15

<210> 170

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 170

Asn Ala Lys Lys Met Glu Asp Met Ile Trp Glu Ser

1 5 10

<210> 171

<211> 27

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 171

Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr Pro

1 5 10 15

Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg

20 25

<210> 172

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 172

Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala Asn Arg

1 5 10

<210> 173

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 173

37

Thr Tyr Thr Pro His Ile Cys Arg His Thr

1 5 10

<210> 174

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 174

Met Lys Lys Lys Asp Ser Thr Ile Phe Ile

1 5 10

<210> 175

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 175

Leu Cys Phe Phe Tyr Leu Leu Tyr

15

<210> 176

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 176

Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu

1 5 10

<210> 177

<211> 75

<212> PROTEIN

<213> Homo sapiens

<400> 177

Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys Lys Lys Lys Asp Lys

1 5 10 15

Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn Ser Thr Glu Cys Gln

20 25 30

Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn Leu Val Gln Leu Asn

35 40 45

Pro His Ser Pro His His Arg Lys Asp Glu Glu Asp His Gln Lys Arg

50 55 60

His His His His Asn Gln Lys Lys Met Ser Val

65 70 75

<210> 178

<211> 55

<212> PROTEIN

<213> Homo sapiens

<400> 178

Ala Ser Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly

1 5 10 15

38

Met Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys

20 25 30

Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp His

35 40 45

Arg Ser Gly Leu Arg Ala Gln

50 55

<210> 179

<211> 68

<212> PROTEIN

<213> Homo sapiens

<400> 179

Pro Tyr Pro Ile Ser Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile

1 5 10 15

Asn Ser Asn Ser Leu Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu

20 25 30

Cys His His Ala Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg

35 40 45

Leu Phe Ser Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala

50 55 60

Leu Cys Lys Phe

65

<210> 180

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 180

Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr Leu Lys Leu Ile Leu

1 5 10 15

Val Met Leu Glu Ile Val Thr Glu

20

<210> 181

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 181

Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg Arg Met Ser Val

1 5 10 15

Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr Val Arg Leu Ser Ala

20 25 30

Val Gly Leu Ser Thr Trp Ser Ala Leu Asp

35 40

39

<210> 182

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 182

Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu His Arg Trp

1 5 10 15

Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg

20 25

<210> 183

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 183

Arg Lys Met Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn

1 5 10

<210> 184

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 184

Ser Arg Lys Lys Gly His Thr Lys Asn Arg Arg Lys Ala Lys Arg Lys

1 5 10 15

Pro Gln Ile

<210> 185

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 185

Asn Lys Ile Ile Cys Lys Gly His Arg Ile Thr

1 5 10

<210> 186

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 186

Ser Ser Phe Phe Tyr Thr Ser Asp Arg

15

<210> 187

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 187

Gln Lys Lys Gly His Met Ile Phe Ser

40

15

<210> 188

<211> 66

<212> PROTEIN

<213> Homo sapiens

<400> 188

Met Leu Gln Lys Gln Cys Cys Tyr Asn Leu Val Ile Lys His Pro Leu

1 5 10 15

Ile Phe Arg Ile Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp Gly Val

20 25 30

Val Gln Ser Leu Gly Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu

35 40 45

Phe Phe Ile Cys Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala

50 55 60

Thr Lys

65

<210> 189

<211> 146

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 189

His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly

1 5 10 15

Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr

20 25 30

Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr

35 40 45

Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala His

50 55 60

Cys Pro Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser

65 70 75 80

Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala

85 90 95

Val Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile Gln

100 105 110

Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser Trp Gly

115 120 125

Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys Arg Trp Pro

41

130 135 140

Arg Arg

145

<210> 190

<211> 78

<212> PROTEIN

<213> Homo sapiens

<400> 190

Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala

1 5 10 15

Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser

20 25 30

Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala Pro Val Gly Ala Gln

35 40 45

Ala Pro Pro Glu Val Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser

50 55 60

Lys Ala Cys Ala Gly Leu Trp Pro Trp Gly Ala Gln Val Arg

65 70 75

<210> 191

<211> 52

<212> PROTEIN

<213> Homo sapiens

<400> 191

Cys His Pro Ala Trp Leu Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu

1 5 10 15

Pro Tyr Pro Phe His Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly

20 25 30

Thr Ala Met Pro Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys

35 40 45

Gly Trp Asp Lys

50

<210> 192

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 192

Met Asp Leu Asp Gly Met Leu Thr Val Ile Gly Gln Gly Leu Val Gly

1 5 10 15

Met Thr Pro Arg Asp Leu Gly Thr Val Ala Gly Ala Thr Ala Gln Ile

20 25 30

Gln Arg Pro Ile Gln Val Gln Thr Gly Gly Arg Leu

42

35 40

<210> 193

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 193

Ser Gln Lys Ser Gln His His Cys Ala Leu Arg Lys Ile Glu Trp Ala

1 5 10 15

Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys

20 25 30

Ala

<210> 194

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 194

Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe Tyr Ile Ser Ser Thr

1 5 10 15

Val Cys Phe Leu Ser Lys His Cys Met Thr Ala Cys Thr

20 25

<210> 195

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 195

Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys Ser

1 5 10

<210> 196

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 196

Leu Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser

1 5 10 15

Arg Ile Ser

<210> 197

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 197

Ser Ile Pro His Val Pro Lys Ala Thr Ala Ala His Ser Val Thr Val

1 5 10 15

43

Lys Leu Gln

<210> 198

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 198

Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly

1 5 10

<210> 199

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 199

Ser Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys

1 5 10 15

<210> 200

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 200

Trp Leu Lys Lys Ser Thr Lys Ser Arg

15

<210> 201

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 201

Asp Arg Val Phe Leu Val Lys Ile

15

<210> 202

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 202

Asn Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala

1 5 10

<210> 203

<211> 106

<212> PROTEIN

<213> Homo sapiens

<400> 203

Gly Ala Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln

1 5 10 15

Thr Pro His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro

20 25 30

44

Arg Ala Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg

35 40 45

Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Trp Pro Pro Ala

50 55 60

Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln

65 70 75 80

Arg Gly Arg Met Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr

85 90 95

Arg Cys Phe Thr Gly Val Arg Thr Ser Ser

100 105

<210> 204

<211> 86

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 204

Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn

1 5 10 15

Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His His Cys Gln

20 25 30

Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp Lys Lys Met

35 40 45

Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp Ser Ser Pro

50 55 60

Leu Gln Leu Leu Leu His Pro His Gly Lys Arg Cys Asn Pro Cys Cys

65 70 75 80

Arg Leu Thr Trp Met Ser

85

<210> 205

<211> 135

<212> PROTEIN

<213> Homo sapiens

<400> 205

Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser Cys Ser Pro

1 5 10 15

Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln Gly Cys Leu

20 25 30

Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly Pro Gly Ser Pro

35 40 45

45

Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg Trp Trp Gly

50 55 60

Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe Pro Pro Pro

65 70 75 80

Asp Pro Pro Trp Pro Val Thr Ala Val Thr Val Met Ala Gly Ser Val

85 90 95

Pro Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly Pro Leu Ala

100 105 110

Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg Glu Met Ser

115 120 125

Ile Phe Leu Pro Gly Ile Phe

130 135

<210> 206

<211> 87

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 206

His Leu Asn Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu

1 5 10 15

Thr Met Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile

20 25 30

Pro Leu Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His

35 40 45

Pro Pro Pro Pro Cys Leu Leu Leu Leu His His Arg His His Leu Ala

50 55 60

Phe Leu Glu Met Ala Cys Ser Ser Gln His Thr His Ser Ile Lys Ile

65 70 75 80

Trp Ile Ser Phe Ser His Glu

85

<210> 207

<211> 89

<212> PROTEIN

<213> Homo sapiens

<400> 207

Leu Ile Leu Ile Gln His Gly Gly Ile Phe Thr Phe Arg Phe Trp Val

1 5 10 15

Glu Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp

20 25 30

46

Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr Ile Ile Glu Thr Asn Val

35 40 45

Phe Val Leu Asn Leu Phe His Val Pro Val Asn Gln Ile Phe Met Met

50 55 60

Ala Phe Tyr Leu Lys Tyr Thr Glu Lys Ala Trp Val Met Glu Leu Glu

65 70 75 80

Trp Leu Ile Gln Gln Gln Cys Tyr Gln

85

<210> 208

<211> 53

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 208

Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn Val Ser Ser Val Leu

1 5 10 15

Lys Pro Phe Leu Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys Thr

20 25 30

Leu Gly Arg Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys Leu

35 40 45

Ile Pro Val Pro Ile

50

<210> 209

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 209

Asp Leu Pro Arg Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln

1 5 10 15

Met Pro Phe Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp

20 25 30

Glu Thr Pro Ser Thr Trp Gly Trp Arg Thr Phe

35 40

<210> 210

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 210

Val Ile Ala Ile Leu Ser Ser Met Cys Gln Glu Ile Leu Asn Arg His

1 5 10 15

47

His Arg Arg Lys Glu Val Arg Lys Leu Lys Gln Ile Leu Phe Gln Val

20 25 30

Glu Glu Thr Lys Ser Gly Arg Phe Trp Ile Lys His Asp Leu Val Ser

35 40 45

Cys Leu Gln Ser Tyr Leu Val Met Glu Ala

50 55

<210> 211

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 211

Trp Leu Gly Met Ala Leu Ile Phe Gly

15

<210> 212

<211> 87

<212> PROTEIN

<213> Homo sapiens

<400> 212

Ala Asn Met Asp Ala Gln Pro Met Met Pro Thr Pro Asn Ile Ile Ala

1 5 10 15

Glu Leu Pro Arg Cys Thr Gly Arg Arg Ser Gly Ser Trp Gly Val Arg

20 25 30

Pro Trp Leu Gly Met Ala Leu Ile Pro Leu Pro Gly Met Arg Gln Pro

35 40 45

Leu Ser Leu Gln Gly Pro Ser Ser Gln Pro Arg Leu Gln Arg Ala Val

50 55 60

Ala Trp His Ser Arg Ser Met Ala Pro Thr Gln Thr Cys Leu Ala Pro

65 70 75 80

His Pro Lys Pro His Trp Asn

85

<210> 213

<211> 53

<212> PROTEIN

<213> Homo sapiens

<400> 213

Thr Ala Leu Lys Tyr Ile Phe Val Ala Val Arg Ala Ile Cys Val Met

1 5 10 15

Lys Ser Phe Leu Ile Phe Arg Arg Trp Lys Ser His Ser Pro Leu Gln

20 25 30

Ile Gln Leu His Leu Ser His Pro Ile Thr Thr Ser Cys Ser Ile Pro

35 40 45

48

Trp Cys His Leu Cys

50

<210> 214

<211> 61

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 214

His Arg Pro Ala Ser Ser Thr Gly Ser Ala Ala Trp Thr Trp Thr Gly

1 5 10 15

Thr Ala Pro Cys Pro Cys Ser Ser Ala Glu Gly Trp Thr Ala Trp Pro

20 25 30

Ser Arg Pro Cys Pro Ser Arg Thr Ala Ser Ala Arg Cys Trp Thr Trp

35 40 45

Ser Ser Arg Gly Leu Lys Gly Arg Ser Arg Cys Arg Thr

50 55 60

<210> 215

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 215

Asn Asp Leu Gln Leu Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr

1 5 10 15

Trp Thr Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr

20 25 30

Thr Ser Leu

35

<210> 216

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 216

Val Ser Trp Tyr Ser His Gln Lys Lys Leu Thr Val Pro His Ser Leu

1 5 10 15

Leu Phe Arg His Lys Ser Arg Arg Leu Arg Leu Cys Tyr Met Ala Asn

20 25 30

trp

<210> 217

<211> 12

<212> PROTEIN

<213> Homo sapiens

49

<400> 217

Val Asn Leu Ser Ser Pro Lys Ala Ser Leu Leu Arg

1 5 10

<210> 218

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 218

Met Asn Ser Val Asn Leu Ser Ser Pro Lys Ala Arg Asp Trp Thr Val

1 5 10 15

Ser Glu Leu Leu Lys Asn

20

<210> 219

<211> 20

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 219

Asn Pro Asp Asn Gly Gly Gly Ser Phe Trp Gln Glu Ala Leu Leu Val

1 5 10 15

Leu Trp Thr Val

20

<210> 220

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 220

Gln Arg Lys Pro Lys Arg Ala Asn Cys Val Ile Gln Arg Arg Ala Lys

1 5 10 15

Met

<210> 221

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 221

Met Gln Lys Lys Trp Pro Ile Thr Tyr Val Ala

1 5 10

<210> 222

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 222

50

Gly Gly Gly Gly Ser Trp Ser Ser Pro Ser Gly

1 5 10

<210> 223

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 223

Gly Lys Asp Arg Tyr Cys Ser Asn Ile Asp Lys His Trp Leu Asn Ser

1 5 10 15

<210> 224

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 224

Arg Ala Asp Pro Gln Arg His Thr

15

<210> 225

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 225

Arg Ala Phe Phe Gly Gly Leu Leu

15

<210> 226

<211> 117

<212> PROTEIN

<213> Homo sapiens

<400> 226

Thr Ser Leu Trp Ser Ser Met Pro His Gly Val Val Thr Ala Asn Ser

1 5 10 15

Trp Leu Pro Phe Gly Ile Asn Trp Glu Arg Arg Thr Arg Thr Met Arg

20 25 30

Thr Ser Ser Ser Pro Arg Trp Thr Arg Leu Pro Thr Arg Trp Arg Pro

35 40 45

Ser Lys Cys Thr Ala Ser Pro His Ser Ser Ser Phe Leu Pro Val Pro

50 55 60

Thr Gly Arg Ser Leu Ile Thr Thr Gly Asn Ala Arg Trp Met Val Leu

65 70 75 80

Arg Asn Ser Trp Arg Ala Val Ala Arg Met Gly Gln Gly Met Met Thr

85 90 95

Ile Ser Arg Thr Trp Lys Lys Gln Arg Ser Gln Thr Trp Arg Lys Thr

100 105 110

Met Ile Arg Lys Leu

51

115

<210> 227

<211> 117

<212> PROTEIN

<213> Homo sapiens

<400> 227

Cys Arg Thr Arg Pro Arg Lys Ala Ala Ser Ala Gln Ser Ser Arg Ser

1 5 10 15

Gly Gly Glu Gly Gly Ser Val Leu Val Gly Met Glu Arg Thr Ala Ser

20 25 30

Thr Gly Glu Thr Val Glu Met Arg Arg Trp Trp Thr Leu Val Met Glu

35 40 45

Trp Lys Ser Ser Tyr Arg Ala Ala Thr Pro Cys Pro Ala Pro Pro Arg

50 55 60

Pro Ala Arg Val Pro Ala His Ala Ser Thr Ser Leu Ser Ser His Ile

65 70 75 80

Thr Leu Lys Pro Val Thr Pro Gln Glu Arg Lys Thr Gln Asp Val Gly

85 90 95

Met Ala Val Ser Met Asp Thr Ile Ser Ile Val Thr Met Trp Gly Glu

100 105 110

Gly Gly Gly Val Gly

115

<210> 228

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 228

Val Ala Arg Val Glu Val Ala Ala Gly Pro Met Arg Glu Val Ala

1 5 10 15

Glu Ala Ala Ala Val Val Met Val Val Arg Pro Val Ala Thr Leu Ser

20 25 30

Pro Gly Glu Ala Arg Ala Pro Leu Glu Ser Val Arg Gln Ile Tyr Ser

35 40 45

Glu His Asn Tyr Cys Arg Leu Ile Leu

50 55

<210> 229

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 229

Arg Leu Pro Gly Leu Thr Cys Lys Val Arg Arg Thr Leu His Gln Leu

52

1 5 10 15

Lys Leu Cys Gln Arg Val Met Ile Leu Ser Val Val Phe Gln Ile Leu

20 25 30

Met Thr Cys Gln Trp Pro

35

<210> 230

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 230

Cys Leu Glu Ser Gly Thr Asp His Ile Leu Thr Glu Ile Leu Ser Ile

1 5 10 15

Ser Cys Lys Lys Met Asn Gln Met Glu Leu

20 25

<210> 231

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 231

Arg Gly Gly Ala Pro Leu Pro Ser Trp Ser Pro Pro Cys Gln Ser Leu

1 5 10 15

Arg Gln Ala Val Thr Ser Ser Arg Thr Ser Gly

20 25

<210> 232

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 232

Ile Ala Ser Thr Phe Tyr Leu Asp Gly Asn Val Ser Ser

1 5 10

<210> 233

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 233

Glu Cys Cys Ser Ser Trp Leu Arg Arg Ile Glu

1 5 10

<210> 234

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 234

Met Glu Cys Ser Lys Leu Lys Leu Phe Asn Leu Phe Trp Thr Trp Gln

1 5 10 15

53

Arg Glu Pro Val Met Gln Pro Gln

20

<210> 235

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 235

Leu Gln Lys Lys Asn Ser Arg Lys Arg Lys Gln Lys Gly

1 5 10

<210> 236

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 236

Leu Val Val Cys Leu Ser Arg Arg Met Lys Arg Cys

1 5 10

<210> 237

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 237

Val Asp Ser Ser Ser Tyr His Pro Phe Lys Ile Asn Gln Asn Ile Arg

1 5 10 15

Phe Gln Lys Phe

20

<210> 238

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 238

Arg Lys Arg Arg Arg Arg Lys Arg Lys Arg Ala Asn

1 5 10

<210> 239

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 239

Pro Tyr Lys Phe Ser Glu Tyr Arg Arg Ala Phe Ser Trp Ser Ala Asp

1 5 10 15

Ile Thr Gly Thr

20

<210> 240

<211> 10

<212> PROTEIN

<213> Homo sapiens

54

<400> 240

Ile Leu Phe Phe Tyr Met Ala Tyr Leu Trp

1 5 10

<210> 241

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 241

Asn Gly Leu Pro Glu Thr Glu Lys Met Pro Gln Leu Met Pro

1 5 10

<210> 242

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 242

Tyr Tyr Gln Lys Thr Trp Arg Val Ile Leu Pro Asp

1 5 10

<210> 243

<211> 97

<212> PROTEIN

<213> Homo sapiens

<400> 243

Ser Thr Trp Phe His Val Thr Ser Gly Thr Ser Leu Ser Cys Ala Leu

1 5 10 15

Pro Ser Val Leu Ala Arg Trp Asn Leu Pro Met Cys Ser Gly Pro Gly

20 25 30

Asn Thr Ser Lys Ser Trp Arg Pro Thr Cys Cys Glu Gln Arg Gly Ser

35 40 45

Arg Ser Glu Ala Ser Cys Arg Asn Gln Lys Leu Lys Arg Asp Ile Ser

50 55 60

Glu Asn Trp Asn Lys Lys Gln Ile Asn Ile Ile Leu Pro Ser Trp Asn

65 70 75 80

Ser Ala Val Cys Gly Phe Pro Cys Leu Ser Pro Lys Leu Ser Arg Gly

85 90 95

Leu

<210> 244

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 244

Ser Ala Ala Trp Arg Ser Cys Ile Ala Leu Trp Cys Ala Ser Ser Val

1 5 10 15

55

Thr Glu Arg Thr Arg Cys Ala Gly Arg Trp Leu Trp Tyr Cys Trp Pro

20 25 30

Thr Trp Leu Arg Gly Thr Ala Trp Gln Leu Val Pro Leu Gln Cys Arg

35 40 45

Arg Ala Val Ser Ala Thr Ser Trp Ala Ser

50 55

<210> 245

<211> 60

<212> PROTEIN

<213> Homo sapiens

<400> 245

Ala Val Thr Arg Ala Ser Ala Pro Arg Ala Thr Ala Ala Ala Gly Ala

1 5 10 15

Trp Arg Gly Arg Arg Pro Pro Thr Ala Arg Ser Ser Ala Thr Thr Arg

20 25 30

Gly Pro Pro Ser Ser Thr Ser Arg Ala Val Gly Arg Pro Pro Cys Trp

35 40 45

Arg Gly Pro Gly Ser Thr Thr His Thr Ser Arg Pro

50 55 60

<210> 246

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 246

Thr Ile Lys Ile Ser Gln Ile Gln Asn Gln Ser Val Asn Lys Asn Phe

1 5 10 15

His Ile His Leu Lys Arg Thr Tyr Gln

20 25

<210> 247

<211> 77

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 247

Ala Glu Thr Ile Thr Gln Leu Tyr Gly Lys His Tyr Ala Ser Gly Trp

1 5 10 15

Met Met Leu Met Ser Lys Thr Pro Arg Thr Tyr Arg Met Cys Thr Val

20 25 30

Gly Met Pro Arg Ser Val Cys Gly Trp Pro Ser Cys Phe Pro Gly Leu

35 40 45

56

Ala Gly Ser Ser Gln Gly Pro Thr Leu Arg Ser Gly Ser His Cys Pro

50 55 60

Gln Asp Cys Arg Arg Asn Val Asn Arg Glu Lys Thr Glu

65 70 75

<210> 248

<211> 63

<212> PROTEIN

<213> Homo sapiens

<400> 248

Ala Ala Leu Gln Thr Gly Pro Gly Ser Cys Pro Pro Ser Arg Ala Ala

1 5 10 15

Ser Leu Leu Phe Ser Ile Arg Arg Trp Ser Thr Ile Ser Cys Pro Arg

20 25 30

Pro Ser Ala Thr Ser Gln Thr Pro Gly Arg Gly Val Pro Pro Arg Ala

35 40 45

Pro Ala Ser Pro Gly Arg Arg Pro Leu Lys Lys Trp Pro Thr Pro

50 55 60

<210> 249

<211> 67

<212> PROTEIN

<213> Homo sapiens

<400> 249

Ala Ser Pro Leu Lys Arg Asp Pro Gln Gln Gly Gln Phe Ile Pro Ser

1 5 10 15

Gln Pro Pro Thr Leu Pro Trp Thr Leu Arg Lys Gln Pro Gln Gly Pro

20 25 30

Ser Cys Ser Arg Gly Ser Gln Arg Ala Val Arg Gly Thr Ser Pro Ser

35 40 45

Gln Gly Ile Leu Gly Ser Thr Gly Cys Ser Leu Gly Ser Arg Leu His

50 55 60

Ser Trp Cys

65

<210> 250

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 250

Asn Gly Lys Leu Ser Pro Arg Thr Ala Val Gly Ala Ala Arg Asn Ile

1 5 10 15

Arg Ser Ser Ser Phe Arg Ser Pro His Thr Ser Pro Phe Phe Gly Pro

20 25 30

57

Thr Ser Gly Cys Leu Gly Phe Thr Gln Glu Pro Pro Leu Pro Gln Phe

35 40 45

Pro Arg Thr Cys His Phe Met Phe Leu Phe

50 55

<210> 251

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 251

Met Lys Ala Leu Ile Ser Gln Glu Met Phe Ser Gln Ala Leu Leu

1 5 10 15

<210> 252

<211> 33

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 252

Asp Thr Thr Phe Gln Arg Ala Pro Leu Lys Phe Lys Leu Ala Leu Lys

1 5 10 15

Arg Val Cys Arg Lys Ser Val Leu Gly Ser Gly Gly Gln Arg Thr Ser

20 25 30

trp

<210> 253

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 253

Ser Phe Leu Val Leu Arg Phe Met Ser Gln Ile Ile Leu Ser Glu Phe

1 5 10 15

Trp Met Cys Cys Gly Leu Val Pro Thr Ser Ser Pro

20 25

<210> 254

<211> 39

<212> PROTEIN

<213> Homo sapiens

<400> 254

Thr Arg Gly Thr Gly Trp Pro Leu Ala Gly Gln Gln Pro Gly Ser Cys

1 5 10 15

Pro Ser Gln His Gln Gly Leu Pro Gly Arg Leu Pro Pro Pro Arg Pro

20 25 30

Leu Gln Arg Val Arg His Thr

35

58

<210> 255

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 255

Cys Arg Arg Leu Val Ser Thr Leu Asn Phe Ser Ala His Leu Leu Met

1 5 10 15

Trp Thr Pro Cys Thr Val Thr Trp Pro Ile Cys Thr Leu Ser Ser Ser

20 25 30

Lys Thr His Ser Thr Ser Met His Met Gln Gln Ser

35 40

<210> 256

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 256

Leu Glu Arg Phe Glu Thr Leu Lys Ile Lys Ser Ser Lys Asn Ile Cys

1 5 10 15

Phe Leu His Val Ser Val Cys Pro Ser

20 25

<210> 257

<211> 16

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 257

Thr Leu Met Met Ser Asn Gly Cys Trp Gly Trp Gln Gln Ser Trp Glu

1 5 10 15

<210> 258

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 258

Ser Leu Leu Lys Arg Asn Met Lys Ser Ser Trp Met Phe Ala Lys Ile

1 5 10 15

Phe Asn Gln Phe Ser Val Thr Ser Ala Trp Lys Asn Ser Trp Ile Gln

20 25 30

Leu Phe Gly Leu Arg Ser Asp Trp Leu Ile Arg Ala Val

35 40 45

<210> 259

<211> 27

<212> PROTEIN

<213> Homo sapiens

59

<400> 259

Ala Gly Ser Thr Gly Ala Ser Arg Pro Leu Arg Thr Arg Arg Thr Arg

1 5 10 15

Ser Gln Cys Arg Arg Ser Trp Ser Pro Ala Arg

20 25

<210> 260

<211> 27

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 260

His Pro Ala Ala Arg Ser Ser Ser Cys Cys Pro Ala Pro Met Ser Leu

1 5 10 15

Ser Trp Gly Thr Ala Leu Met Thr Ser Pro Arg

20 25

<210> 261

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 261

Arg Ser Gln Asn His Gln Lys Ser Pro Gln Ala Leu Ala Met Pro Trp

1 5 10 15

<210> 262

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 262

Arg Ser Gln Asn His Gln Lys Arg Lys Val Leu Gly Thr Ile Leu Val

1 5 10 15

His Asn His Ile Ile Asp Tyr Ala Trp Val Ile Cys His Leu

20 25 30

<210> 263

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 263

Ile Ser Phe Gly Tyr Pro Arg Trp Thr Leu Lys Ala Leu Val Tyr Ser

1 5 10 15

Gln Asn Gly Val Leu Ile Trp Leu Met

20 25

<210> 264

<211> 8

<212> PROTEIN

60

<213> Homo sapiens

<400> 264

Lys Glu Lys Lys Gly Gly Ser Glu

15

<210> 265

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 265

Glu Leu Leu His Pro Ser Gln Arg Leu Leu Val Pro

1 5 10

<210> 266

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 266

Val Ile Phe Phe Ser Lys Pro Lys Arg Ile Lys Asn Leu Ser Leu Lys

1 5 10 15

Thr

<210> 267

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 267

Lys Ser Ala Arg Thr Ala Leu Asn Ser Glu Leu Asp Val Gln Asn Ala

1 5 10 15

Val His Glu Asn Gln Lys Glu Arg Val Phe

20 25

<210> 268

<211> 19

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 268

Ile Ile Leu Asp Thr Ala Lys Leu Leu Thr Ser Pro Gln Ser Leu Leu

1 5 10 15

Pro Ile Arg

<210> 269

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 269

61

Arg Ile Leu Leu Gly Thr Phe Leu Ala Ile Pro

1 5 10

<210> 270

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 270

Gly Cys Cys Arg Leu Leu Thr Glu Met Gly Lys Arg Lys Arg Lys Arg

1 5 10 15

Arg Ser His Trp Met Lys Ala Gln

20

<210> 271

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 271

Leu Ile Asn Pro Asp Lys Lys Ile Lys Met Ile

1 5 10

<210> 272

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 272

Asn Lys Lys Lys Val Asn Lys Asn

15

<210> 273

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 273

Phe Ile Phe Phe Leu Ile Leu Trp Ser Ser Arg Gln

1 5 10

<210> 274

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 274

Met Lys Lys Glu Asn Lys Ile Lys Trp Lys Leu Asn

1 5 10

<210> 275

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 275

Lys Glu Ser Pro Gly Arg Glu Lys Gly Met Glu Lys Met

1 5 10

62

<210> 276

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 276

Leu Ala Phe Ser Ser Leu Leu Trp Pro Ser His Leu Asp Met Ser Met

1 5 10 15

Leu Gln

<210> 277

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 277

Lys Pro Asn Lys Asn Thr Gln Ile Thr Phe Ser Leu Met Ser Phe Thr

1 5 10 15

Leu

<210> 278

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 278

Arg Phe Phe Pro Ser Ile Leu Ile Lys Ser Tyr Ile

1 5 10

<210> 279

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 279

Gly Ser Pro Pro Gly Gly Pro Arg Pro Cys Trp Thr

1 5 10

<210> 280

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 280

Ser Ile Gly Tyr Leu Thr Gly Phe Cys Ser Pro Asn Ala

1 5 10

<210> 281

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 281

Glu Glu Pro Pro Ser Cys Arg Pro Thr Ala His Ile Pro

63

1 5 10

<210> 282

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 282

Glu Gln Lys Thr Glu Trp Glu Trp Lys Leu Gln Ile Glu

1 5 10

<210> 283

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 283

Lys Val Ala Phe Leu Cys Glu Lys Glu Gln Ile Val

1 5 10

<210> 284

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 284

Gln Leu Lys Lys Ala Val Lys Val

15

<210> 285

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 285

Leu Gln Arg Met Ser Ser Asp Lys Arg Asn Thr Gly

1 5 10

<210> 286

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 286

Ile Asn Lys Lys Ile Gln Ala Thr Lys

15

<210> 287

<211> 93

<212> PROTEIN

<213> Homo sapiens

<400> 287

Thr Ser Ser Pro Arg Thr Met Ser Trp Leu Cys Cys Leu Gly Gln Ile

1 5 10 15

Met Ala Ser Ala Val Glu Ala Ser Gln Pro Pro Thr Trp Pro Ser Met

20 25 30

64

Pro Arg Arg Thr Pro Val Gly Pro Val Ser Ser Pro Ala Arg Gln Glu

35 40 45

Gly Val Arg Val Cys Ser Gly Cys Val Thr Cys Gly Glu Thr Ala Pro

50 55 60

Met Ala Ala Met Thr Thr Ala Ala Ala Pro Cys Ser His Pro Gln Ser

65 70 75 80

Trp Pro Val Ser Leu Ser Arg Trp Arg Cys Ala Ser Val

85 90

<210> 288

<211> 112

<212> PROTEIN

<213> Homo sapiens

<400> 288

Val Gly Lys Pro Leu Pro Arg Ser His Thr Ala Gln Asn Ile Arg Glu

1 5 10 15

His Thr Gln Glu Arg Asp Pro Leu Ser Ala Val Asn Ala Gly Asn Arg

20 25 30

Leu Val Arg Ser His Thr Ser Met Tyr Ile Glu Lys Cys Thr Gln Glu

35 40 45

Lys Asp Arg Ile Val Ala Glu Asn Val Glu Asn Pro Ser Ala Arg Ser

50 55 60

His Ala Ser Ile Asn Ile Gly Glu Leu Thr Gln Glu Arg Ser Pro Met

65 70 75 80

Gly Ala Met Asn Val Gly Lys Leu Ser Thr Arg Ser Gln Thr Ser Ala

85 90 95

Asp Ile Arg Lys Phe Met Leu Gly Arg Met Pro Thr Gly Met Lys Thr

100 105 110

<210> 289

<211> 59

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 289

Cys Arg Ala Ser Leu Arg Thr Asp Cys Leu Leu Ala Val Val Ile Gln

1 5 10 15

Phe Gln Ile Ser Thr Tyr Gln Ala Gln Leu Arg Ile Ser Leu Arg Lys

20 25 30

Lys Lys Thr Lys Gln Asp Ile Leu Thr His Ile Ile Glu Cys Leu Lys

35 40 45

65

Lys Met Lys Thr Ser Thr Ser Leu Gly Met Ile

50 55

<210> 290

<211> 94

<212> PROTEIN

<213> Homo sapiens

<400> 290

Val Ala Pro Cys Pro Trp Ser Trp Gly Ser Leu Arg Met Leu Val Gln

1 5 10 15

Pro Glu Met Arg Ala Pro Tyr Gly Ser Val Leu Thr His Cys Gln Arg

20 25 30

Leu Met Thr His Tyr Cys Ala Met Leu Gly Gln Leu Ser Ala Glu Ala

35 40 45

Lys Leu Arg Gly Arg Arg Gly Gly Gly Ala Ala Pro Gln Pro Val Pro

50 55 60

Ala Ser Asn Arg Val Ala Ala Ala Val Ser Gln Glu Asp Ala Gly Leu

65 70 75 80

Val Glu Glu Pro Met Glu Asp Val Val Glu Asp Gly Pro Gly

85 90

<210> 291

<211> 82

<212> PROTEIN

<213> Homo sapiens

<400> 291

Asn Thr His Leu Gln Leu Thr His Cys Phe Leu Glu Ile Lys Pro Tyr

1 5 10 15

Ser Thr Lys Lys Met Lys Met Ala Cys Gly Lys Arg Ser Leu Leu Gln

20 25 30

Glu Val Lys Lys His Glu Leu Pro Lys Lys Ala Pro Ser Trp Pro Asp

35 40 45

Arg Pro Leu Val Gly Leu Ala Arg Ile Gln Ile Trp Ile Leu His Leu

50 55 60

Leu Gly Leu Arg Pro Ser Leu Leu Ser Val Phe Gln Met Thr Phe

65 70 75 80

Ile Lys

<210> 292

<211> 88

<212> PROTEIN

<213> Homo sapiens

<400> 292

66

Gly Gly Phe Arg Gln Trp Trp Cys Thr Cys Arg Met Ser Gly Pro Ser

1 5 10 15

Cys Leu Leu Trp Arg Ser Gly Arg Pro Cys Ala Ser Arg Lys Leu Gln

20 25 30

Arg Gln Leu Pro Gln Pro Arg Arg His Lys Gly Lys Arg Ser Leu Leu

35 40 45

Asn Arg His Leu Met Pro Trp Ser Lys Gln Gln Thr Leu Leu Asp Gly

50 55 60

Thr Gln Lys Leu Gln Arg Pro Pro His Ser Arg Lys Arg Thr Leu Ile

65 70 75 80

Ser Gln Arg Pro Leu His Pro Pro

85

<210> 293

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 293

Leu Ala Gly Leu Leu Cys Pro Ala Leu His Leu Val Lys Pro Arg Val

1 5 10 15

Leu His Gln Asn Leu Asp Pro Thr Ser Ser Ser Phe Ser Lys Trp Cys

20 25 30

Arg Pro Trp Leu Glu Gln Met Leu His Ser Cys Arg Ile Gln Lys Ser

35 40 45

Asp Phe Ser Asn Asn Trp Asn Ser Ser Thr Gln Trp Gly Ser

50 55 60

<210> 294

<211> 127

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 294

Asn Val His Arg Gln Pro Val Gln Ile Ile Lys Asn Met Glu Thr Thr

1 5 10 15

Val Thr Tyr Arg Lys Lys Gln Ile Gln Ser Gln Ser Ser Thr Ser Ile

20 25 30

Leu Ser Ala Leu Arg Val Ser Pro Val Arg Asn Ser Pro Cys Ser Met

35 40 45

Cys Cys Val Ile Ile Pro Pro Gly Ser Ser His Ala Cys Val Gln Lys

50 55 60

67

Val Thr Ser Ser Leu Gly Ile Arg Ala Gln Gly Thr Arg Arg Ser Ser

65 70 75 80

Asn Lys Pro Leu Arg Gly Asp Pro Gly Val Ile Val Pro Cys Leu Cys

85 90 95

Leu Glu Glu Pro Arg Lys Gly Leu Trp Glu Arg Ser Pro Gly His Pro

100 105 110

Arg Gly Ser Gln Ser Ala Leu Gly Thr Ala Ser Glu Gly Trp Ser

115 120 125

<210> 295

<211> 16

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 295

Asn Val His Arg Gln Pro Val Gln Gln Ile Gln Ser Gln Ser Ser Thr

1 5 10 15

<210> 296

<211> 14

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 296

Val His Arg Gln Pro Val Gln Ile Gln Ser Gln Ser Ser Thr

1 5 10

<210> 297

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 297

Ser Arg Pro Gly Trp Lys Arg Thr Gln Gln Arg Pro Pro Trp Arg Thr

1 5 10 15

Ser Arg Gly Gln Ala Gln Asp Thr Ser Gly His Arg Gly Ser

20 25 30

<210> 298

<211> 53

<212> PROTEIN

<213> Homo sapiens

<400> 298

Ala Ala Ala Leu Gly Leu Arg Ile Leu Pro Pro Pro Pro Leu Thr Ser Pro

1 5 10 15

Ser Arg Ser Thr Thr Ala Pro Val Gly Ser Gln Cys Val Pro Ser Trp

20 25 30

68

Thr Ala Arg Arg Trp Ser Thr Thr Ala Ser Gly Trp Arg Arg Trp Thr

35 40 45

Thr Ala Arg Pro Pro

50

<210> 299

<211> 37

<212> PROTEIN

<213> Homo sapiens

<400> 299

Leu Ser Ala Ile Arg Lys Met Thr Tyr Phe Thr Ser Trp Ile Leu Asp

1 5 10 15

Ser Asp Ser Thr His Tyr Ile Pro Ser Cys Asn Arg Lys Ser Cys Tyr

20 25 30

Lys Arg Met Trp Ser

35

<210> 300

<211> 54

<212> PROTEIN

<213> Homo sapiens

<400> 300

Trp Glu Thr Leu Ser Thr Trp Arg Pro Ser Glu Arg Trp Ser Cys Arg

1 5 10 15

Ser Pro Ala Ser Leu Pro Arg Met Trp Cys Trp Cys His Gln Ala Ser

20 25 30

Met Pro Trp Arg Ala Thr Pro Pro Leu Leu Gly Ala Thr Thr Ser Pro

35 40 45

Pro Asp Ala Ser Gly Thr

50

<210> 301

<211> 42

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 301

Tyr Lys Gly Arg Lys Glu Arg Lys Thr Ser Val Gln Gly Ile Thr Thr

1 5 10 15

Val Ile Leu Lys Arg Arg Thr Ser Leu Arg Arg Glu Ser Phe Met Lys

20 25 30

Asn Phe Leu Ala Val Thr Ile Thr Gly Ser

35 40

69

<210> 302

<211> 67

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 302

Arg Pro Pro Met Thr Thr Trp Pro Arg Met Arg Met Thr Leu Arg Trp

1 5 10 15

Ala Arg Gly Ser Leu Pro Gly Pro Ala Arg Ala Arg Pro Thr Thr Pro

20 25 30

Leu Leu Pro Trp Arg Thr Thr Cys Ser Ser Arg Pro Arg Val Gly Ala

35 40 45

Ser Arg Pro Ala Pro His Arg Pro Gln Arg Ser Ser Arg Arg Tyr Ser

50 55 60

Arg Ser Ala

65

<210> 303

<211> 71

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 303

Arg Ser Thr Gly Leu Thr Gly Glu Gln Thr His Trp Leu Arg Pro Thr

1 5 10 15

Ser Gly Pro Ala Thr Met Ser Pro Trp Tyr Arg Gly Pro Thr Pro Ser

20 25 30

Pro Leu Thr Cys Arg Cys Thr Thr Pro Pro Ala Ser Pro Trp Leu Pro

35 40 45

Ile Pro Val Arg Pro Met Gly Ala Arg Ala Pro Ala Pro Thr Cys Val

50 55 60

Ser Ser Thr Thr Thr Gly Ser

65 70

<210> 304

<211> 40

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 304

Arg Ser Leu Pro Ala Gly Pro Ser Ser Arg Arg Arg Ala Pro Pro Arg

1 5 10 15

70

Ser Gly Arg Gly Ser Leu Arg Lys Trp Arg Pro Gly Ala Ser Arg Arg

20 25 30

Trp Arg Met Ser Thr Trp Pro Ser

35 40

<210> 305

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 305

Val Ser Thr Ser Trp Gly Pro Thr Ala Gly Ala Ala Arg Ala Arg Thr

1 5 10 15

Cys Ser Pro Cys Ser Pro Thr Thr Pro Ser Pro Ala Ser Gly Ser Thr

20 25 30

Ser Arg Arg Thr Arg Pro Asn Arg Val Leu Thr

35 40

<210> 306

<211> 45

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 306

Thr Trp Pro Ala Ala Cys Trp Ala Thr Trp Thr Ser Pro Gly Ala Ala

1 5 10 15

Val Pro Val Ala Leu Arg Pro Ser Ala Ser Trp Ser Arg Cys Thr Cys

20 25 30

Ser Ser Ser Ala Thr Met Trp Arg Ser Thr Thr Pro Arg

35 40 45

<210> 307

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 307

Arg Arg Ser Arg Ala Glu Cys Gly Ser Thr Arg Ser Leu Thr Ala Arg

1 5 10 15

Arg Glu Thr Pro Thr Ala Thr Met Cys Ala Ala Pro Arg Val Ser Pro

20 25 30

<210> 308

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 308

Met Val Lys Arg Lys Asn Pro Lys Arg Arg Lys Leu Gln Glu Leu Leu

71

1 5 10 15

Lys Arg Arg Lys Leu Arg Lys Asn Ser Asn Leu Ser His Met Met Ile

20 25 30

Arg Phe Phe Trp Met Glu Met Arg Cys Met Tyr Gly Ser Met Thr Gln

35 40 45

Phe Thr Leu Lys His Leu Ser Trp Asp

50 55

<210> 309

<211> 35

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 309

Arg Glu Pro Leu Val Leu Ser Val Gly Asn Leu Ile Gly Leu Pro Ile

1 5 10 15

Met Pro Ser Arg Cys Glu Asn Ser Ser Leu Gly Gly Met Leu Glu Met

20 25 30

Ser Met His

35

<210> 310

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 310

Leu Pro Gly Gly Ile Ala Ser Arg Phe Pro Ala Ser Trp Pro Arg Thr

1 5 10 15

Pro Ala Ala Cys His Pro Ser Leu Arg Thr Gly Thr

20 25

<210> 311

<211> 66

<212> PROTEIN

<213> Homo sapiens

<400> 311

Gly Met Thr Ser Thr Cys Pro Ser Ala Met Thr Trp Ala Thr Ser Ser

1 5 10 15

Pro Cys Ser Ala Thr Glu Arg Ala Thr Ser Ala Gly Val Trp Thr Lys

20 25 30

Met Ala Glu Arg Cys Arg Ala Pro Ala Pro Ser Gln Ala Pro Pro Leu

35 40 45

Arg Val Tyr Pro Pro Ser Leu His Pro Trp Ser Gly Pro Arg Pro Gly

50 55 60

72

Gln Met

65

<210> 312

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 312

Gly Gly Arg Met Leu Leu Glu Met Met Lys Arg Leu Val Arg Lys Asn

1 5 10 15

Leu Lys Lys Thr Met Met Ile Leu Lys Thr Ser Leu Lys Met Ser Phe

20 25 30

Gln Lys Met Arg Lys Arg

35

<210> 313

<211> 47

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 313

Ser His Ile Trp Pro Lys Gly Phe Glu Ala Gln Arg Ala Gln Glu His

1 5 10 15

Arg Ala Gln Leu Val Cys Ser Ala Leu Leu Pro Glu Gly Ile Arg Cys

20 25 30

Arg Pro Gly Ala Pro Ala Ala Val Ser Pro Pro Arg Gly Ser Gln

35 40 45

<210> 314

<211> 50

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 314

Gln Thr Ser His Trp Cys Pro Thr Gln Pro Arg Arg Val Ala Ser Arg

1 5 10 15

Val Trp Pro Ser Thr Thr Thr Leu Ser Cys Pro Pro Arg Ser Ser Thr

20 25 30

Ser Cys Ser Thr Ala Ala Thr Ala Pro Ser Pro Cys Thr Pro Arg Ala

35 40 45

Ala Glu

50

73

<210> 315

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 315

Ser Asn Cys Gln Leu Val Thr Ala Val Pro Asn Trp Asn Leu Phe Ser

1 5 10 15

Thr Val Leu Leu His Leu

20

<210> 316

<211> 36

<212> PROTEIN

<213> Homo sapiens

<400> 316

Asn Asp Gly Gly Gly Ser Arg Ser Trp His Leu Pro Ser His Pro Met

1 5 10 15

Gln Gln Thr Pro Thr Thr Ala Arg Pro Ser Thr Gln Thr Ser Trp Pro

20 25 30

Ser Trp Pro Ser

35

<210> 317

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 317

Leu Ile Leu Glu Lys Ser Pro Ile Asn Val Lys Lys Cys Lys Gly Phe

1 5 10 15

Asn His Tyr Ser Phe Cys Gln Lys His Glu Gln Ser His Thr

20 25 30

<210> 318

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 318

Asn Pro Pro Val Lys Glu Ser Gln Val Ala Arg Arg Lys Cys Leu Lys

1 5 10 15

Glu Met Ala Arg Arg Lys Lys Thr Glu Glu

20 25

<210> 319

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 319

Leu Ala Met Thr Asp Asp Gln Arg Met Val Lys Asn Lys Gln Leu Leu

1 5 10 15

74

Met

<210> 320

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 320

Met Leu Thr Gln Thr Asp Ser Ala Trp Asp Leu Glu Ile Ala Glu Val

1 5 10 15

Leu Phe His Ile Gln

20

<210> 321

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 321

Ala Phe Arg Asp Arg Asn Thr Arg Ile Leu Gly Arg Val Ser Ala Pro

1 5 10 15

Glu Ala Ser His Asp Thr Val Thr Phe Arg Thr Ala Arg Lys Gly Glu

20 25 30

Lys Phe

<210> 322

<211> 54

<212> PROTEIN

<213> Homo sapiens

<400> 322

Gly Lys Leu Phe Arg Arg Ser Lys Phe Glu Ala Lys Pro Thr Ser Gly

1 5 10 15

Pro Trp Gln Pro Gly Arg Asn Ile Thr Thr Lys Leu Ile Gln Arg Pro

20 25 30

Leu Arg Ser Ser Leu Gly Ser Arg Lys Thr Pro Ser Leu Pro Phe

35 40 45

Leu Gly Glu Glu Glu Leu

50

<210> 323

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 323

Ser Ser His Met His Asn Thr Ser Pro Cys Gln Met Met Ser His Leu

1 5 10 15

75

Asn His Val Gln Phe Val Leu Tyr Lys Leu

20 25

<210> 324

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 324

Cys Tyr Leu Ala Gly Ser Lys Met Thr Ser Leu Thr Ser Gly Trp Asn

1 5 10 15

Gly Lys Arg Gln Ser Cys Ser Gln Leu Cys Leu Leu Pro Cys Thr Ile

20 25 30

<210> 325

<211> 73

<212> PROTEIN

<213> Homo sapiens

<400> 325

Arg Arg Gly Thr Ser Gln Arg Ala Gln Ser Gly Pro Leu Ser Phe Val

1 5 10 15

Leu His Phe Leu Ala Lys Asp Pro His Pro Met Phe Leu Cys Thr Pro

20 25 30

Arg Ala His Pro Leu Ala Arg Pro Gln Gly His Leu Leu Ala His Pro

35 40 45

Gly Pro Ser Pro Thr Trp Pro Gly Pro Leu Pro Leu Pro Gln Thr Arg

50 55 60

Leu Asp Leu Ser Ala Gly Pro Thr Ala

65 70

<210> 326

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 326

Ser Ser Ile His Ile Val Glu Lys Gln Leu Lys Leu Arg Glu Arg Pro

1 5 10 15

Thr Asn Lys Lys Lys Arg Lys Asn

20

<210> 327

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 327

Leu Gln Lys Lys Ala Pro Gln Lys Arg Ile Lys Leu Leu Arg Met Leu

1 5 10 15

76

Pro Ala Leu Tyr Leu Met Ala Cys Gln

20 25

<210> 328

<211> 40

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 328

Ala Ala Gln Met Ser Trp Ser Leu Pro Cys Pro Pro Gly Gly Ala Glu

1 5 10 15

Glu Arg Met Glu Ala Pro Ser Val Gly Val Ala Arg Arg Arg Arg Arg

20 25 30

Ser Lys Gly Ser Ser Met Arg Cys

35 40

<210> 329

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 329

Met Thr Pro Gly Pro Gly Ala Gly Pro Ala Ala Cys Ser Ser Cys Ala

1 5 10 15

Pro Ala Leu Cys Ala Ala Ala Ala

20

<210> 330

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 330

Thr Ser Arg Gly Ser Ser Leu Gln Ala Ser Ser Trp Lys Met Ala Ala

1 5 10 15

Leu Phe Leu Thr Thr Thr Ser Arg Lys Ser Arg Pro Cys Thr Trp Ser

20 25 30

Cys Ala

<210> 331

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 331

Phe Glu Phe Phe Leu Gln Cys Pro Ala Lys

1 5 10

<210> 332

<211> 24

77

<212> PROTEIN

<213> Homo sapiens

<400> 332

Leu Cys Thr Ile Cys Thr Ser Tyr Val Ser Pro Trp Ile Ile Phe Thr

1 5 10 15

Glu Met Glu Tyr Phe Thr Glu Met

20

<210> 333

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 333

Cys Gly Lys Ser Ile Trp Trp Leu Lys Glu Leu Arg Val Leu Met Phe

1 5 10 15

Leu His Leu Trp Asp Ser Met Lys Gln Val Ala Leu His Gln Ser Asn

20 25 30

Arg

<210> 334

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 334

Ala Ser Pro Ser Pro Ser Arg Asn Lys Ser Glu Leu Leu Thr Ser Pro

1 5 10 15

Arg Pro Leu Thr Leu Gln Ser

20

<210> 335

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 335

Leu Lys Asp Cys Ile Ser Thr Lys Pro Ile Ser Gly Ile Val Pro

1 5 10 15

<210> 336

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 336

Val Ser Glu Asp Gly Lys Val Ala Ser Asp Thr Cys Val Thr Leu His

1 5 10 15

Trp Pro Leu Gly Lys Phe Arg Phe Arg

20 25

78

<210> 337

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 337

Lys Phe Cys Asn Phe Ile Leu Leu Phe Ser Leu Ile Leu Ser Ile Lys

1 5 10 15

His Ile Ser Val Phe Phe His Ile Phe Ser Ala Val Ile Leu Thr Gln

20 25 30

Tyr Arg Ser

35

<210> 338

<211> 13

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 338

Gly Arg Cys Tyr Gln Leu Pro Gln Leu Gln Gly Phe Leu

1 5 10

<210> 339

<211> 11

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 339

Ser Lys Leu Tyr Gly Asp His Phe Leu Leu Leu

1 5 10

<210> 340

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 340

Ala Arg Ala Ile Ala Thr Arg Pro Gly Gly Trp Asn Arg Met Gly Glu

1 5 10 15

Pro Arg Gly Phe Cys

20

<210> 341

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 341

Glu Glu Leu Phe Leu Leu Val Ser Leu Ala Met Phe Arg

1 5 10

79

<210> 342

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 342

Ile Pro Ser Arg His Met Phe Ser Val Thr Lys Ile Met Leu Lys Glu

1 5 10 15

Thr Phe Lys Arg Arg Lys

20

<210> 343

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 343

Arg Arg Lys Ile Ile Pro Asn Cys Phe Trp Gly Lys Thr Glu Ser Pro

1 5 10 15

Ser Ile Phe Thr Thr Cys

20

<210> 344

<211> 41

<212> PROTEIN

<213> Homo sapiens

<400> 344

Arg Thr Thr Thr Thr Ser Leu Lys Glu Glu Glu Gly Ile Ile Leu Ile

1 5 10 15

Thr Glu Ser Arg Ser Leu Ala Leu Leu Phe Gln Ser Leu Met Ile Leu

20 25 30

Ile Cys Ala Gly Thr Lys Glu Pro Phe

35 40

<210> 345

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 345

Ser Ser Gly Gly Gly Leu Gly Leu Ile Met Leu Pro Trp Ser Gly Val

1 5 10 15

Glu Ile Ile Ile Cys Leu Lys Arg Ile Val Lys Arg Arg Lys Arg Lys

20 25 30

Asn

<210> 346

<211> 11

<212> PROTEIN

<213> Homo sapiens

80

<400> 346

Thr Thr Lys Lys Ser Met Ile Glu Trp Lys Ile

1 5 10

<210> 347

<211> 40

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 347

Ala Gly Ser Ala Thr Lys Met Ser Thr Asn Ala Ala Arg Arg Thr Gly

1 5 10 15

Ala Ala Ser Arg Ser Ala Thr Thr Ser Arg Val Ala Ser Thr Val Pro

20 25 30

Ala Met Ala Gly Pro Ala Lys Thr

35 40

<210> 348

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 348

Gly Ser Lys Lys Thr Gly Ile Met Trp

15

<210> 349

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 349

Arg Glu Lys Arg Gln Ala Val Asn Met Met Cys Gln His Thr Met Ile

1 5 10 15

<210> 350

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 350

Gly Leu Ser Leu Ser Tyr Gly Pro Val Ser Pro Ile Ile Arg Arg Leu

1 5 10 15

Trp Asn Ile Phe Val Arg Asn Gln Glu Lys Ile

20 25

<210> 351

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 351

Ser Ser Cys Phe Gln Arg Arg Lys Arg Arg Ile Tyr Gln His Leu Arg

81

1 5 10 15

Tyr

<210> 352

<211> 68

<212> PROTEIN

<213> Homo sapiens

<400> 352

Ala Leu Pro His His Pro Ser Pro Ile Ala Arg Ala Ser Trp Gly Leu

1 5 10 15

Arg Pro Pro Ala Phe Leu Trp Ala Leu Arg Ile Leu Thr Pro Leu Pro

20 25 30

Leu Pro Gln Pro Gln Pro Pro Ala Val Ala Thr Ala Thr Val Ala Ala

35 40 45

Thr Ala Ala Ala Leu Leu Gly Leu Cys Pro Phe Pro His His Pro Ile

50 55 60

Trp Pro Glu Val

65

<210> 353

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 353

Ser Pro Ser Leu Gln Thr Gln Trp Arg Leu Lys Arg Ser

1 5 10

<210> 354

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 354

Thr Leu Val Met Val Ala Thr Val Lys Leu Phe Leu

1 5 10

<210> 355

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 355

Leu Val Cys Ser Ala Leu Ile Ser Leu Thr Leu Lys Gly Asn Leu Phe

1 5 10 15

Thr Leu Leu Thr Leu Leu Met

20

<210> 356

<211> 40

82

<212> PROTEIN

<213> Homo sapiens

<400> 356

Val Ala Phe His Pro Ser Gln Pro Gly Pro Cys Gly Arg Pro Thr Pro

1 5 10 15

Lys Leu Pro Ser Pro Met Arg Arg Gln Pro Ser Trp Gln Trp Ala Ala

20 25 30

Ser Pro Ser Thr Ala Thr Thr Ser

35 40

<210> 357

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 357

Leu Val Ile Pro Lys Leu Ile Ile Leu Met Glu Lys Thr Leu Thr Gly

1 5 10 15

Cys Leu Arg Ala Leu Lys Met Asn Arg Phe

20 25

<210> 358

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 358

Thr Lys Ser Gly Lys Lys Gln Glu Leu Thr Phe Leu Leu Leu

1 5 10

<210> 359

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 359

Glu Ser Pro Pro Ala Pro Ser Met Pro Arg Cys

1 5 10

<210> 360

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 360

Arg Gln Thr Leu Arg Thr Leu Ser Leu Arg Gly Met Val Pro Arg Thr

1 5 10 15

Leu

<210> 361

<211> 10

<212> PROTEIN

<213> Homo sapiens

83

<400> 361

Thr Thr Ser Gln Ser Gln Leu Ser Ser Glu

1 5 10

<210> 362

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 362

Glu Met Lys Lys Thr Thr Phe Ile Ser

15

<210> 363

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 363

Arg Phe Gln Met Gln Pro Leu Ser Leu Met Pro Ala His Trp Ser Ile

1 5 10 15

Cys Leu Leu Arg Asp Ser Met Ile Trp

20 25

<210> 364

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 364

Val Trp Lys Lys Met Lys Ser Lys Thr Ser Cys Val Val

1 5 10

<210> 365

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 365

Ser Gly Arg Asn Leu Pro Thr Ala Pro Cys

1 5 10

<210> 366

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 366

Thr Trp Pro Thr Gln Thr Trp Thr Arg Leu Pro Arg Arg Ser Cys Cys

1 5 10 15

Ser Arg

<210> 367

<211> 13

<212> PROTEIN

84

<213> Homo sapiens

<400> 367

His Thr Ser Thr Leu Gly Lys Lys Ser Ile Leu Leu Leu

1 5 10

<210> 368

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 368

Gly Ile Tyr Phe Met Leu Gln Thr Pro Thr Ile Thr Arg Leu Lys Leu

1 5 10 15

Trp Ile Gln Lys Gln Lys Thr Val Gln His

20 25

<210> 369

<211> 13

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 369

Leu Ala Ser Ser Tyr Ser Gly Ile Gln Gln Arg Lys Leu

1 5 10

<210> 370

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 370

Ile His His Leu Pro Gly Thr Ile Pro Leu Ser Ser Gly Ala Arg Met

1 5 10 15

Cys Leu Leu Ser Gln Ala Pro Pro Ala Gly Ser Gly Gly Arg Arg

20 25 30

<210> 371

<211> 55

<212> PROTEIN

<213> Homo sapiens

<400> 371

Gly Ser Pro Ser Arg Ser Ala Ala Cys Gly Gly Arg Glu Arg Arg Asp

1 5 10 15

Arg Arg Pro Gly Pro Ser Arg Ala His Cys Pro Ala Asn Gly Ala Gly

20 25 30

Trp Pro Cys Pro Ala Val Pro Leu Gly Pro Ala Arg Thr Gly Leu Leu

35 40 45

Pro Gly Glu Ala Ala Pro Ser

50 55

85

<210> 372

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 372

Cys Trp Thr Ser His Leu Met Gln Met Thr Arg Pro Trp Leu Asn

1 5 10 15

<210> 373

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 373

Gln Arg Lys Lys Ser Leu Lys Pro Ser Gly Ser Leu

1 5 10

<210> 374

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 374

Arg Thr Pro Pro Gly Gly Gly Arg

15

<210> 375

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 375

Glu Glu Lys Lys Lys Ala His Leu

15

<210> 376

<211> 15

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 376

Ala Thr Val Pro Thr Cys Ser Pro Arg Thr Leu Arg Cys Leu Pro

1 5 10 15

<210> 377

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 377

Ile Gly Asp Arg Glu Gln Val Lys Lys Thr Met

1 5 10

<210> 378

<211> 9

86

<212> PROTEIN

<213> Homo sapiens

<400> 378

Leu Ser Lys Lys Glu Lys Met Thr Arg

15

<210> 379

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 379

Leu Ser Ile Asn Asn Tyr Arg Phe Gln Met Lys Phe Tyr Phe Arg Phe

1 5 10 15

Thr Ser His Gly Ser Pro Phe Thr Ser Ala Asn Phe

20 25

<210> 380

<211> 31

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 380

Ala Pro Phe Ser Pro Lys Val Trp Arg Thr Ala Arg Gly Ala Ser Leu

1 5 10 15

Ser Cys Gln Thr Thr Pro Pro Ile Thr Leu Ser Ser Thr Pro Arg

20 25 30

<210> 381

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 381

Asp Tyr Lys Lys Asn Thr Gly Ile

15

<210> 382

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 382

Ile Val Ala Leu Met Ser Ser Thr Ile Ile

1 5 10

<210> 383

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 383

Ile Pro Phe Phe Thr Gly Leu Thr Arg Met Met Arg

1 5 10

87

<210> 384

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 384

Glu Gly Arg Ser Ser Thr Asn Ala Arg Ser Ala Thr Val Cys Leu Thr

1 5 10 15

Ala Gly Thr Ser Ser Lys Ile Thr Trp

20 25

<210> 385

<211> 14

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 385

Ser Phe Ile Ala Leu Ile Gly Arg Leu Met Phe Gln Ala Cys

1 5 10

<210> 386

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 386

Asn Thr Met Lys Trp Trp Glu Leu Tyr Leu Val Ile Leu Ser His Ile

1 5 10 15

Ala

<210> 387

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 387

Ser Lys Pro Lys Arg Ile Lys Asn Leu Ser Leu Lys Thr

1 5 10

<210> 388

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 388

Ser Leu Asn Gln Arg Ser Tyr Ala Arg Pro Ser Cys Gln Pro

1 5 10

<210> 389

<211> 16

<212> PROTEIN

<213> Homo sapiens

88

<400> 389

Met Ser Pro Pro Phe Met Pro Ser Arg Val Leu Phe Met Ser Arg Arg

1 5 10 15

<210> 390

<211> 26

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 390

Ile Arg Val Ile Glu Val Ala Arg His Lys Pro Leu Leu Arg Asn Leu

1 5 10 15

Lys Lys Lys Lys Ala Arg Ile Leu Arg Lys

20 25

<210> 391

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 391

Pro Ala Gly Trp Pro Gln Ala Ala Pro Arg Pro Ser Ala

1 5 10

<210> 392

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 392

Met Ala Thr Ser Ser His Arg Phe Leu Glu Asp Met Leu Pro Ala Lys

1 5 10 15

<210> 393

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 393

Glu Ser Pro Pro Ala Ser Leu Lys Asn

15

<210> 394

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 394

Gly Pro Pro Pro Pro Ala Pro Pro Ile Ser Leu Ala Ser

1 5 10

<210> 395

<211> 10

<212> PROTEIN

<213> Homo sapiens

89

<400> 395

Thr Gln Lys Lys Ser Tyr Pro Ile Lys Thr

1 5 10

<210> 396

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 396

Asn Trp Ile Ser Lys Lys Leu Asn Leu Thr Met

1 5 10

<210> 397

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 397

Leu Tyr Lys Lys Asn Ser Arg Arg Leu

15

<210> 398

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 398

Ser Ile Phe Leu Ala Lys Glu Ser Ser Gly Arg Ala Tyr Val

1 5 10

<210> 399

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 399

His Gln Arg Cys Ala Ala Ser Gln Gln Ser Ala Ser Phe

1 5 10

<210> 400

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 400

Asp Leu Lys Lys Arg Arg Ser Ala Val

15

<210> 401

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 401

Gln Leu Arg Leu Met Leu Pro Ser Ser Ala Arg Pro Trp Gly Pro Asp

1 5 10 15

gly

90

<210> 402

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 402

Asn Tyr Asn Lys Glu Phe Leu His Gln Gly Gln Leu Glu Asn

1 5 10

<210> 403

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 403

Leu Gln Thr Trp Lys Lys Asn His Leu Thr Gln Thr Thr Leu Lys

1 5 10 15

<210> 404

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 404

His Ile Thr Gly Gly Thr Gly Leu Leu Leu

1 5 10

<210> 405

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 405

Glu Glu Gly Gly Cys Ala Ser Gly Thr Trp

1 5 10

<210> 406

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 406

Asn Gln Glu Arg Ser Pro Asp Pro Ser Pro Asp Leu His Thr Gly Leu

1 5 10 15

Ile Lys Ser Gln Arg Lys Thr Asn Thr Asp Val Asn Phe

20 25

<210> 407

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 407

Ala His Gln Lys Arg Lys Gln Ser Cys Arg Cys Ser

1 5 10

91

<210> 408

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 408

Cys His Lys Lys Thr Ser Gln Leu Asp

15

<210> 409

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 409

Arg Glu Phe Ile Asn Leu Lys Met Ile Lys Trp Thr Leu Leu Lys Ser

1 5 10 15

<210> 410

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 410

Ile Phe Tyr Thr Trp Thr Asn Gln Tyr Leu Gln Asn

1 5 10

<210> 411

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 411

Ser Leu Ser Pro Gly Pro Lys Arg Thr Gln Ile Trp Ser

1 5 10

<210> 412

<211> 22

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 412

Asn Trp Met Thr Met Gln Thr Ile Ile Lys Asn Arg Thr Glu Ala Met

1 5 10 15

Pro Arg Glu His Leu Leu

20

<210> 413

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 413

Glu Phe Phe Phe Thr Ile Gly Ile Thr Trp Ile

1 5 10

92

<210> 414

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 414

Phe Pro Phe Phe Ser Ser Leu Glu Gln Cys Pro

1 5 10

<210> 415

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 415

Leu Tyr Lys Lys Ile Glu Lys Asn

15

<210> 416

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 416

His Pro Ser Ser Thr Asp Gly Gly Leu Cys Glu Ala Pro Gly Leu Pro

1 5 10 15

Thr Ser Ser Ser Leu

20

<210> 417

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 417

Leu Pro Leu Gln Pro Tyr Leu Leu Pro Cys Leu Leu

1 5 10

<210> 418

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 418

Leu Ile Glu Gln Asn Leu Ile Gly Lys Lys Asn

1 5 10

<210> 419

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 419

Glu His Gly Gly Pro Ser Ser Met Pro Leu

1 5 10

<210> 420

<211> 11

<212> PROTEIN

93

<213> Homo sapiens

<400> 420

Thr His Trp Lys Arg Asn Pro Lys Ala Ile Trp

1 5 10

<210> 421

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 421

Gly Tyr Thr Pro Gln Met Glu Ala Gly Ala Gly Gln Trp

1 5 10

<210> 422

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 422

Leu Thr Lys Lys Ile Arg Asn Ser Cys Leu Ile

1 5 10

<210> 423

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 423

Gln Pro Pro Arg Arg Pro Ser Thr Ala Asn

1 5 10

<210> 424

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 424

Ala Pro Lys Lys Thr Leu Leu Lys Cys Trp

1 5 10

<210> 425

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 425

Asp Met Lys Lys Asn Cys Gly Ser Leu

15

<210> 426

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 426

Trp Thr Thr Gly Val Pro Pro Ser Ser

15

94

<210> 427

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 427

Asp Ser Leu Lys Met Val Lys Asn Phe Leu Ser

1 5 10

<210> 428

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 428

Phe Leu Asn Pro Arg Lys Leu Gly Pro

15

<210> 429

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 429

Lys Thr Pro Pro Gly Gly Tyr Pro Leu

15

<210> 430

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 430

Ser Pro Cys Trp Ala Trp Thr Pro Gly Gly Trp Ala Ala Ser

1 5 10

<210> 431

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 431

Gln Val Pro Ser Ile Leu His

15

<210> 432

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 432

Val Lys Pro Thr Leu Trp Glu Glu Gly Thr Ser

1 5 10

<210> 433

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 433

95

Tyr Trp Ala Pro His Val Thr Pro

15

<210> 434

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 434

Leu Leu His Phe Leu Val Met Leu Arg Ile Ile

1 5 10

<210> 435

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 435

Asn Thr Leu Lys Asn Ser Phe Leu Leu Trp Pro

1 5 10

<210> 436

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 436

Glu Arg Gln Lys Ile Lys Lys Lys Pro Lys Arg

1 5 10

<210> 437

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 437

Gly Arg Arg Lys Arg Ser Glu Gly Gly Val Lys Arg Lys Lys Glu Asn

1 5 10 15

Val Cys Lys Arg Arg Lys Arg Asn Val Gly Glu Lys Lys Arg Lys Gly

20 25 30

Phe Asp Gly Arg Lys Arg Lys Gly Asp Gly

35 40

<210> 438

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 438

Tyr Met Lys Lys Asn Leu Thr Pro Ser Phe Ala Met Lys Tyr Leu Pro

1 5 10 15

Pro Ile Cys Thr Leu Gly Asp Gln

20

<210> 439

96

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 439

Tyr Ser Ala Ser Gly His Gly Val Arg Ile Thr Lys Ser

1 5 10

<210> 440

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 440

Glu Met Phe Val Leu Asn Pro Cys Leu Pro Ser Ile Met Leu Thr Ser

1 5 10 15

His Asn Met

<210> 441

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 441

Val Pro Ala Leu Lys Lys Leu Arg Ser Pro Leu Trp Ile Phe

1 5 10

<210> 442

<211> 55

<212> PROTEIN

<213> Homo sapiens

<400> 442

Arg Gln Pro Glu Ala Ala Thr Lys Ser Cys Val Arg Ser Ser Cys Gly

1 5 10 15

Gly Pro Ser Gly Asp Glu Pro Pro Pro Cys Leu Gln Gln Pro Asp Pro

20 25 30

Arg Ala Leu Ser Gln Ala Phe Ser Arg Ser Phe Pro Leu Phe Pro Ser

35 40 45

Leu Ala Gly Lys Ser Met Ile

50 55

<210> 443

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 443

Arg Thr Pro Thr Ser Pro Ala Ala Thr Lys Ser His Ser Leu Arg Val

1 5 10 15

Phe Gly Trp Ala Asp Ala Leu Leu Asn Leu Val Ala Arg Val Pro Ser

20 25 30

97

Asn Leu Gly Asp Leu Phe Lys Ser Lys Val Asn Pro Ala Thr Met Leu

35 40 45

<210> 444

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 444

Arg Glu Pro Arg Glu Arg Leu Tyr Gln Asp Thr Ser Leu Met Met Ile

1 5 10 15

Val Leu Thr Ile Gln Asn Ala Ala Phe Leu Ser Asn Ile Arg Met Ala

20 25 30

<210> 445

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 445

Ser Lys Thr Gln Arg Arg Tyr Lys Leu Ser Ser Leu Lys Glu Asp Leu

1 5 10 15

Leu Gln Arg Ser Ala Phe Thr Ser Gln Ala Pro Leu Tyr

20 25

<210> 446

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 446

Pro Gly Leu Arg Pro Ser Gln His Leu Leu Leu Tyr Ile Thr Leu Arg

1 5 10 15

Val Val Lys Trp Ile Pro Ala Leu Ala Thr Ala Ser Ala Trp Pro Ala

20 25 30

Pro Ser Ala Lys Thr Val Trp His Pro Thr Leu Leu Ile

35 40 45

<210> 447

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 447

Cys His Leu Ala Ser Cys Pro Gln Cys Phe His Gln Trp Gly Arg His

1 5 10 15

His His Ser His Arg Tyr Gln Glu Trp Tyr Leu Arg

20 25

<210> 448

<211> 11

<212> PROTEIN

<213> Homo sapiens

98

<400> 448

Gly Ala Thr Pro Gln Ala Ala Pro Thr Thr Ser

1 5 10

<210> 449

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 449

Val Leu Thr Lys Gln Lys Val Leu Tyr Lys

1 5 10

<210> 450

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 450

Ile Lys Ala Leu Leu Thr Leu Asn Ser Leu

1 5 10

<210> 451

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 451

Gly Thr Pro Pro Trp Met Pro Phe Arg

15

<210> 452

<211> 49

<212> PROTEIN

<213> Homo sapiens

<400> 452

His Arg Pro Pro His Ser Gln Gln Pro Phe His Ser His Cys Pro Ser

1 5 10 15

Arg Ser Pro Gln Pro Ser Leu Arg Pro His Pro His Pro Leu Arg Ala

20 25 30

Gln Gly Cys Asn Pro Ser Leu Ser Thr Thr His Arg Trp Tyr Ser Trp

35 40 45

gly

<210> 453

<211> 64

<212> PROTEIN

<213> Homo sapiens

<400> 453

Asn Tyr Gly His Cys Asn Ser Pro Leu Ala Phe Leu Thr Gln Leu Leu

1 5 10 15

99

Pro Arg Pro Thr Ser Asn Pro Trp Trp Met Thr Arg Arg Asp Pro Phe

20 25 30

Cys Gly Gly Asn Leu Ile Ser Thr Lys Leu Glu Asn Phe Val Ser Gly

35 40 45

Ile Ser Ala Gly Thr Glu Arg Arg Gln Met Asn Leu Cys Phe Leu Tyr

50 55 60

<210> 454

<211> 51

<212> PROTEIN

<213> Homo sapiens

<400> 454

Arg Lys His Cys Cys Lys Arg Ile Thr Leu Arg Ser Ser Cys Ser Leu

1 5 10 15

Gly Arg Asp Leu Tyr Leu Trp Asn Pro Arg Ile Leu Lys Gly Ser Gly

20 25 30

Gly Tyr Thr Asp Val Ile Met Met Asp Ala Thr Lys Cys Thr Leu Lys

35 40 45

Ala Pro Thr

50

<210> 455

<211> 47

<212> PROTEIN

<213> Homo sapiens

<400> 455

Asn Ser Gly Ser Gln Ser Gly Thr Lys Met Lys Ala Thr His Ser Gly

1 5 10 15

Gly Leu Lys Lys Lys Ser Phe Thr Val Pro Lys Leu Leu Phe Pro Thr

20 25 30

Gln Lys Phe Asn Leu Asp Leu Lys Ala Cys Ser Ile Pro Glu Asp

35 40 45

<210> 456

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 456

Arg Asp Gln Cys Phe Gln Met Met Glu Gly Lys Ala Phe Leu Pro Glu

1 5 10 15

Arg Lys Cys Phe Pro Gly Asn Gln His Pro Leu Gly

20 25

<210> 457

<211> 43

<212> PROTEIN

100

<213> Homo sapiens

<400> 457

Ala Met Arg Arg Pro Ser Ser Arg His Ala Ala His Arg Pro Leu Cys

1 5 10 15

Gln Arg Phe Pro Thr Leu Thr Val Ser Arg Trp Met Thr Ser Leu Ile

20 25 30

Ser Ser Leu Arg Val Glu Arg Ser Pro Ser Pro

35 40

<210> 458

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 458

Cys Ile Trp Gln Pro Val Met Asp Thr Val Ile Leu Tyr Arg Ser Tyr

1 5 10 15

Cys Ser Thr Arg Gln Thr Ser Met Gln

20 25

<210> 459

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 459

Asn Ser Leu Ala Leu Cys Trp Val Pro Ala Cys Trp Val Thr Cys Leu

1 5 10 15

His

<210> 460

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 460

Leu Leu Gln Pro Cys Leu Lys Ala Leu Ser Val Pro Leu Trp Lys Met

1 5 10 15

Pro Ser Arg Ala Ser

20

<210> 461

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 461

Asn Pro Pro Leu Arg Phe Asn Leu Ile Leu Met Met Tyr

1 5 10

<210> 462

101

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 462

Thr Ala Leu Gly Ala Thr Ile Pro Ser Arg Pro Arg Ser Ala Ala Ala

1 5 10 15

Leu Arg Thr

<210> 463

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 463

Asp Ala Lys Lys Leu Val Leu Leu Asn Trp Leu Pro

1 5 10

<210> 464

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 464

Gly Gly Arg Met Arg Asn Ile Phe Thr Pro Ala Gly His Lys

1 5 10

<210> 465

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 465

Leu Cys Arg Asn Met Lys Gln Gln Ile Tyr

1 5 10

<210> 466

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 466

Gly Glu Trp Ala Ala Leu Gly Pro Cys Trp Lys Ser Ala Cys Arg Ala

1 5 10 15

Ser Arg Ser Ala

20

<210> 467

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 467

Cys Pro Gly Gly Ala Leu Gln Trp

15

102

<210> 468

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 468

Glu Ala Lys Lys Arg Ile Met Trp Leu Leu

1 5 10

<210> 469

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 469

His Thr Ser Trp Asn Phe Arg Pro Leu Asn Trp Arg Ser

1 5 10

<210> 470

<211> 36

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 470

Val Gly Ala Gln Leu Met Leu Thr Ser Thr Ser Cys Ser Thr Met Ser

1 5 10 15

Cys Leu Ser Arg Pro Gly Trp Ala Ala Leu Gln Arg Leu Thr Trp Val

20 25 30

Cys Arg Arg Pro

35

<210> 471

<211> 87

<212> PROTEIN

<213> Homo sapiens

<400> 471

Arg Ala Pro Pro Lys Thr Ala Pro Glu Thr Ala Ala Lys Phe Leu Lys

1 5 10 15

Glu Gly Thr Glu Arg Asn Thr Ala Glu Ser Leu Gln Glu Lys Leu Gly

20 25 30

Val Thr Pro Gly Ser Trp Trp Met His Leu Arg Ser Arg Phe Leu Ser

35 40 45

Ala Asn Ala Ala Cys Glu Cys Val Arg Arg Leu Pro Cys Asp Gly Asn

50 55 60

Thr Leu Pro Arg Arg Thr Ser Asn Phe Tyr Val Asp Val His Glu Ala

65 70 75 80

Leu Val Phe Asp Val Cys Leu

85

103

<210> 472

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 472

Gln Leu Leu Ser Gln Thr Pro Arg Leu Pro Pro Cys Leu His Thr Glu

1 5 10 15

Glu Pro Ser His Trp Thr Gly Gly Pro Arg Ser Pro Pro

20 25

<210> 473

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 473

Ile Ala Phe His Ile Lys Ser Ile Ala Ala Ala Leu Lys Arg His Phe

1 5 10 15

Thr Val Asn Met Ser Lys Val Glu Thr Gly Leu His Val

20 25

<210> 474

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 474

Trp Lys Lys Thr Gln Ala Asp Cys Phe Phe Gly Leu Leu Lys Lys Ile

1 5 10 15

Gly Leu Pro Gln Cys Gly Asp Ser Phe Leu Lys Arg Pro Leu Thr

20 25 30

<210> 475

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 475

Ala Val Phe Ala Ser Ser Arg Pro Thr Ser Pro Ala Ser Cys Arg Arg

1 5 10 15

Pro Pro Ser Ser Trp Trp Arg

20

<210> 476

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 476

Thr Gln Arg Arg Leu Lys Asn Trp Lys Glu Lys Lys Cys Ile Glu Leu

1 5 10 15

104

Asn Ile Leu Ile Ser Trp Arg Ile

20

<210> 477

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 477

Arg His Asn Asp Gly Lys Asn Glu Lys Asn Trp Pro Asn Ser Met Lys

1 5 10 15

Pro Ser Tyr Gly Ser Val Ala Thr Ala Ala Ser Ser Gly His Cys Ile

20 25 30

Leu Cys Leu Val Trp Arg

35

<210> 478

<211> 46

<212> PROTEIN

<213> Homo sapiens

<400> 478

Thr Arg Glu Lys Ile Lys Gln Pro Val Ala Gln Trp Val Met Ile Thr

1 5 10 15

Lys Arg Pro Lys Glu Asn His Gln Arg Lys Arg Arg Asn Val Asn Ile

20 25 30

Lys Lys Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln

35 40 45

<210> 479

<211> 50

<212> PROTEIN

<213> Homo sapiens

<400> 479

Leu Pro Thr Pro Phe Cys Thr Thr Leu Arg Asn Phe Phe Leu Ser Ser

1 5 10 15

Leu Ile Leu Ala Val Leu Cys Thr Asn Cys Ile Phe Arg Tyr Thr Ala

20 25 30

Ser Phe Leu Gln Ala Phe Met Leu Pro Gly Phe Leu Lys His Thr Lys

35 40 45

Arg Lys

50

<210> 480

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 480

Leu Pro Ile Leu Met Met Pro Ser Lys Lys Leu Ile Leu Tyr Leu Phe

105

1 5 10 15

Leu Cys Cys Pro Thr Leu Ser Phe Trp Gln Arg Glu Gln Pro Ser Arg

20 25 30

Thr

<210> 481

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 481

Leu Ser Met Ile Met Tyr Ser Leu Pro Leu Thr Tyr Ser Asn Leu His

1 5 10 15

Thr Leu Thr Ile Leu Lys Lys Tyr Met Leu Ser Gln Asn

20 25

<210> 482

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 482

Cys Thr Pro Phe Cys Thr Leu Ser Ala Lys His Val Pro Leu Ser Ile

1 5 10 15

Ser Gly Ser Pro Ala Leu Glu Leu Leu Gly Pro Trp

20 25

<210> 483

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 483

Leu Asn Gln Lys Asn Ile Pro Asp Leu Ser Ile

1 5 10

<210> 484

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 484

Val Ser Pro Leu Ala Pro Cys Pro Pro Leu Glu Val His Leu His

1 5 10 15

<210> 485

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 485

Ser Thr Gly Arg Leu Thr Ala Arg Arg Met Pro Pro Cys Leu

1 5 10

106

<210> 486

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 486

Arg Pro Lys Lys Arg Thr Val Leu

15

<210> 487

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 487

Val Val Lys Val Trp Met Arg Val Lys Tyr Thr Lys Glu Val Ile Met

1 5 10 15

Asp Leu Thr Asn Val

20

<210> 488

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 488

Phe Arg Phe Phe Arg Met Arg Lys Trp Arg Asp Gln Ile

1 5 10

<210> 489

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 489

His Phe Trp Asn Ser Thr Lys Lys Arg Leu Arg Thr Met Lys Ile Lys

1 5 10 15

Cys Ser

<210> 490

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 490

Leu Pro Val Leu Ala Pro Ile Met Ser Arg Gly Arg Ala

1 5 10

<210> 491

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 491

Met Thr Lys Lys Glu Asn Ala Asn Met Ile Phe Lys Lys

1 5 10

107

<210> 492

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 492

Ala Leu Lys Lys Pro Leu Arg Lys Val Arg Arg Leu

1 5 10

<210> 493

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 493

Asp Gln Phe Phe Trp Lys Met Gln Leu Leu

1 5 10

<210> 494

<211> 196

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 494

Ala Pro Thr Val Leu Gly Gly Pro Arg Trp Leu Cys Cys Thr Gln Asn

1 5 10 15

Val Ala Ser Gly Asn Gly Pro Leu Leu Ala Arg Pro Ala Gly Lys Pro

20 25 30

Arg Ala Ala Ser Leu Ser Ser Trp Lys Ala Leu Gly Pro Ser Ala Ala

35 40 45

Ser Leu Arg Pro Ser Leu Arg Ala Pro Gly Arg Pro Val Gly Ala Ser

50 55 60

Arg Pro Ala Gln Thr Gln Gly Ser Leu Pro Arg Arg Leu Leu Pro Pro

65 70 75 80

Phe Cys Gly Gln Thr Trp Pro Gly Ser Trp Gly Ser Gln Glu Ser Phe

85 90 95

Ile Cys Cys Ala Asp Ile Ser Thr Ala Leu Pro Leu Gly Ser Ser Arg

100 105 110

Pro Ala Pro Ala Pro Arg His Arg Glu His Glu His Gly His Gln Ala

115 120 125

Arg Pro Pro Arg Leu Leu Phe Thr Ser Leu Met Gln Leu Leu Trp Thr

130 135 140

Gln Leu Thr Arg His Ser Pro Thr Leu His Thr Gly Pro His Pro

145 150 155 160

108

Ser Leu His Val Ala Ala Leu Ser Leu Leu Arg Met Asp Ser Thr Leu

165 170 175

Arg Gln Gly Ala Ser Leu Phe Ser Leu Thr Leu Gly Gly Pro Gly Pro

180 185 190

Trp Lys Asp Pro

195

<210> 495

<211> 120

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 495

Arg Ser Val Cys Ser Tyr Ala Gly Ser Trp Gly Thr Ala Leu Gly Arg

1 5 10 15

Gly Gln Ser Leu Trp Arg Arg Arg Lys Arg Trp Leu Cys Ala Gln Thr

20 25 30

Val Arg Ala Ala Thr Ile Leu Leu Ala Arg Arg Arg Arg Arg Ser Leu

35 40 45

Asp Leu Arg Lys Arg Arg Arg Ala Asn Arg Arg Met Met Met Met Ile

50 55 60

Gln Arg Ser Leu Asn His Leu Leu Ser Ser Trp Lys Thr Gly Ala Trp

65 70 75 80

Lys Thr Leu Thr Thr Cys Ser Gln Arg Arg Ile Ile Glu Pro Ser Pro

85 90 95

Thr Thr Arg Pro Ser Ala Ser Leu Ser Asp Pro Ser Leu Leu Pro Lys

100 105 110

Ile Pro Arg Leu Leu Ser Pro Arg

115 120

<210> 496

<211> 9

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 496

Arg Ser Trp Gly Thr Ala Leu Gly Arg

15

<210> 497

<211> 104

<212> PROTEIN

<213> Homo sapiens

109

<400> 497

Ser Gly Pro Thr Arg Arg His Ser Trp Ala Pro Lys Thr Ser Ser Leu

1 5 10 15

Thr Arg Asn Pro Arg Arg Ser Leu Ala Ser Pro Thr Arg Gly Lys Gly

20 25 30

Ser Ala Arg Gly Cys Gly Arg Ser Arg Thr Thr Leu Leu Ser Arg Leu

35 40 45

Pro Ala Ile Ser Pro Pro Arg Lys Arg Ala Val Trp Lys Ser Leu Asn

50 55 60

Gln Ser Pro Lys Leu Gln Arg Val Thr Val Ile Arg Arg Gly Met Gln

65 70 75 80

Arg Ala Ala Ala Thr Arg Lys Gly Ser Trp Ser Leu Met Ser Gln Pro

85 90 95

Arg Arg Arg Thr Arg Lys Glu Arg

100

<210> 498

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 498

His Leu Thr Thr Lys Gly Glu Gly Trp Val His Gln Trp Gly Val Thr

1 5 10 15

Val Gly Ala Gln Val Ala Thr Ala Pro Ser Met Gly Thr Pro His Pro

20 25 30

Leu Pro His His Pro Ser Met Ala Leu Thr Pro Thr Ala Leu Cys Ser

35 40 45

Trp Ser Met Ala Trp Ile Asn Leu Arg

50 55

<210> 499

<211> 64

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 499

Thr Cys Arg Ala Ile Val Thr Met Thr Ser Cys Leu Tyr Leu Arg Thr

1 5 10 15

Thr Glu Gly His Pro Val Gly Ala Ile Pro Pro His Ser Ala Ser Tyr

20 25 30

Pro Thr Tyr Pro Thr His Phe Ser Thr Trp Glu Pro Val Pro Ser Asp

110

35 40 45

Arg Pro Ala Gly Ala Leu Pro Arg Cys Ser Pro Leu Arg Thr Glu Gly

50 55 60

<210> 500

<211> 75

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 500

Arg Arg Arg Lys Arg Arg Asp Ile Gln Glu Asn Gln Arg Thr Leu Leu

1 5 10 15

Lys Ile Gln Asn Cys Thr Tyr Pro Gly Ser Pro Ala Trp Arg Leu Val

20 25 30

Ala Phe Leu Ser Leu Val Ala Arg Leu Trp Lys Ala Ser Asp Leu Ser

35 40 45

Val Arg Lys Arg Asn Thr Tyr Gly Trp Lys Leu Ser Met Ala Arg Val

50 55 60

Ile Glu Asn Ser Ala Ser Lys Gln Lys Ile His

65 70 75

<210> 501

<211> 60

<212> PROTEIN

<213> Homo sapiens

<400> 501

Gly Cys Phe Trp Pro Cys Leu Trp Asn Ser Leu Leu Arg Phe Cys Pro

1 5 10 15

Ala Ser Cys Ser Gly Cys Tyr Ser Trp Thr Ile Pro Pro Thr Gly Ser

20 25 30

Pro Phe Ser Cys Thr Thr Thr Arg Ser Ser Met Asn Pro Thr Ser Leu

35 40 45

Thr Pro Gly Arg Ser Ser Arg Thr Thr Ser Gln Leu

50 55 60

<210> 502

<211> 52

<212> PROTEIN

<213> Homo sapiens

<400> 502

Cys Thr Thr Ser Arg Gly His Cys Thr Gly Ser Pro Arg Pro Cys Thr

1 5 10 15

Pro Phe Ala Ile Ser Cys Thr Ala His Lys Trp Asn Gly Lys Thr Pro

20 25 30

111

Thr Trp Ser Leu Pro Lys Ser Thr Ser Arg Trp Lys Gly Pro Ser Ser

35 40 45

Cys Cys Arg Arg

50

<210> 503

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 503

Lys Gln Thr Arg Arg Arg Lys Leu Leu Leu Leu Cys Lys Lys Asn Gln

1 5 10 15

Ile Leu Lys Lys Lys Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp

20 25 30

gly

<210> 504

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 504

Cys Pro Leu Leu Leu Val Tyr Ile Met Ser Thr Thr Gly Trp Leu Glu

1 5 10 15

Arg Ala Tyr Gly Arg Leu Trp Arg Pro Thr Ala Ile Arg Gln Thr Leu

20 25 30

Tyr

<210> 505

<211> 52

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 505

Ser Leu Leu Val Pro Arg Arg Leu Leu Pro Tyr Thr Leu Pro His Ser

1 5 10 15

Ala His Gly Ser Arg Arg Thr Ser Thr Cys Gln His Pro Thr Thr Thr

20 25 30

Asn Thr Ser Ser Ser Ser Ser Ser Gln Met Trp Cys Arg Pro Arg Ser

35 40 45

Ser Cys Ile Arg

50

112

<210> 506

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 506

Ser Leu Pro Pro Leu Met Ser Ala Val Ala Thr Leu Cys Gln Asn Thr

1 5 10 15

Pro Met Val Ser Pro Leu Gly Ala Pro Leu Ala Arg Asn Cys Thr Gln

20 25 30

His Pro Ser Pro Leu Trp Leu Gly Thr Leu Arg Asp Thr

35 40 45

<210> 507

<211> 71

<212> PROTEIN

<213> Homo sapiens

<400> 507

Gln Arg Lys Ile Met Asp Thr Arg Gln Gly Lys Val His Thr Ser Thr

1 5 10 15

Asp Ser Ser Trp Arg Ala Pro Ala Gly Thr Pro Lys Gln Glu Pro Leu

20 25 30

Leu Lys Pro Val Ser Arg Ser Trp His Ala Leu Cys Arg Ser Ser Leu

35 40 45

Gln Lys Pro Pro Pro Trp Thr Asp Lys Lys Pro Asn Arg Pro Thr Ser

50 55 60

Ala Leu Cys Ile Glu Pro Asn

65 70

<210> 508

<211> 41

<212> PROTEIN

<213> Homo sapiens

<400> 508

Met Lys Leu Thr Leu Ala Val Leu Pro Thr Leu Arg Asn Leu His Cys

1 5 10 15

Tyr Pro Gln Leu Cys Leu Gln Gly Arg Leu Ala Ala Arg Asn Ala Asn

20 25 30

Met Asn Leu Glu Ile Leu Leu Gly Val

35 40

<210> 509

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 509

113

Cys Ser Ile Lys Pro Pro Leu Pro Arg Arg Trp Thr Thr Val Leu Arg

1 5 10 15

Glu Pro Trp His Gly Met Gln Glu Ile Arg Pro

20 25

<210> 510

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 510

Arg Thr Gly Pro Trp Thr Pro Leu Leu Pro Pro Ser Cys Pro Trp

1 5 10 15

<210> 511

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 511

Asn Met Glu Thr Phe Gln Ser Met Ser Trp Pro Ile Leu Thr Ile Phe

1 5 10 15

Leu Thr Ser Met Arg Thr Ile Ile Pro Glu Phe Cys Leu Asn Glu Phe

20 25 30

<210> 512

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 512

Leu Gly Gln Ile Ile Met Lys Lys Met Arg Arg Pro Val Cys Tyr Lys

1 5 10 15

<210> 513

<211> 37

<212> PROTEIN

<213> Homo sapiens

<400> 513

Leu Pro Pro Pro Ser His Arg Ala Pro His Ser Pro Pro Pro Ser Gln

1 5 10 15

His Arg Arg Ala Gln Asp Gln Pro His Ser Cys Leu Cys Pro Arg Arg

20 25 30

Thr Gly Cys Cys Val

35

<210> 514

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 514

Ser Val Ser Arg Pro Glu Ala Cys Gly Ile Arg Thr Ser Leu Leu Val

1 5 10 15

114

Arg Arg Met Arg Arg Gln

20

<210> 515

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 515

Leu Gln Lys Lys Glu Ile Ala Lys Asn Val Phe Val Gln Asn Ile

1 5 10 15

<210> 516

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 516

Ile Ile Lys Lys Asn Pro Ala Leu Pro Lys His

1 5 10

<210> 517

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 517

Gly Phe Lys Met Gln Ser Arg Arg Arg Lys Trp Ala Gly Asn

1 5 10

<210> 518

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 518

Ser Ala Thr Thr Thr Thr Val Ile Leu Glu Ile Ile Ile Met Asp Arg

1 5 10 15

Val Ile Pro

<210> 519

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 519

Ala Ala Ser Gly Ala Ala Glu Ala Ala Glu Leu Leu

1 5 10

<210> 520

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 520

Ile Thr Lys Lys Arg Asn Trp Leu Arg Ser Trp Leu

115

1 5 10

<210> 521

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 521

Arg Arg Lys Asn Lys Lys Gln Pro Thr Phe Phe Leu Trp Gln

1 5 10

<210> 522

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 522

Val Thr Ile Arg Ile Gln Lys Gln Lys Phe Arg Ile Leu Lys Arg Ile

1 5 10 15

<210> 523

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 523

Arg Lys Glu Asn Thr Val Lys Arg Pro Asn Leu Pro His Leu

1 5 10

<210> 524

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 524

Ile Glu Lys Lys Glu Arg Lys Asn Leu

15

<210> 525

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 525

Leu Thr Leu Phe Ser Leu Lys Val Trp Glu

1 5 10

<210> 526

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 526

Ile Leu Ile Val Cys Arg Lys Ser Phe Tyr Ser

1 5 10

<210> 527

<211> 11

<212> PROTEIN

<213> Homo sapiens

116

<400> 527

Leu Ile Ser His Leu Met Lys Trp Ala His Pro

1 5 10

<210> 528

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 528

Ser Leu Phe Phe Ser Tyr Cys Phe

15

<210> 529

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 529

Thr Leu Asn Trp Lys Arg Asn Leu Leu Phe Gly

1 5 10

<210> 530

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 530

Thr Leu Asn Trp Lys Arg Gly Pro Ser Val Val Pro Trp

1 5 10

<210> 531

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 531

Thr Leu Asn Trp Lys Arg Glu Pro Arg Leu Ser Tyr Leu Lys Thr Met

1 5 10 15

Tyr Ser Ser Leu Phe Trp Ser Gln Phe Pro Phe Leu Gln Cys Cys His

20 25 30

His His His Leu His His His Tyr His His Val Leu Asn Lys Tyr Ile

35 40 45

<210> 532

<211> 226

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 532

Gln Thr Gln Leu Pro Lys Val Trp Asp Pro Val Phe Phe Gly Ala Ala

1 5 10 15

117

Ser Pro Pro Ser Cys Thr Trp Ala Asp Trp Leu Cys Gly Ala Trp Ala

20 25 30

Cys Ala Ala Thr Cys Ser Gln Pro Ser Ser Ser Arg Gln His Arg Ser

35 40 45

Ala Leu Met Thr Phe Leu Leu Ala Ser Trp Cys Val Pro Lys Val Ser

50 55 60

Cys Gln Gly Val Arg Arg Pro Trp Asn Ser Leu Pro Arg Ser Thr Trp

65 70 75 80

His Ser Val Leu Met Cys His Gln Leu Cys Ser Asp Thr Ser Ser Ile

85 90 95

Val Ala Gly Gln Ala Thr His Gln Trp Pro Gly Ser Cys Pro Arg Cys

100 105 110

Ala Ser Arg Pro Arg Arg Glu Arg Thr Ala Ala Trp Gln Leu Cys Cys

115 120 125

Arg Arg Pro Ser Arg Thr Thr Phe Arg Ser Gln Gln Pro Ser Trp Gln

130 135 140

Gly Cys Cys Pro Gly Ser Thr Gly Ala Cys Trp Leu Ser Ala Arg His

145 150 155 160

Leu Arg Arg Pro Cys Ser Gly Ala Arg Pro Val Pro Ala Gly Val Trp

165 170 175

Pro Ala Ala Ser Ala Ser Thr Ser Thr Pro Ser Arg Gln Leu His Arg

180 185 190

Val Arg Pro Arg Ala Cys Met Pro Cys Pro Gly Ser Ser Gly Ser Ser

195 200 205

Gly Ala Cys Thr Arg Cys Arg Arg Ser Gly Trp Leu Gly Arg Leu His

210 215 220

Val Ala

225

<210> 533

<211> 88

<212> PROTEIN

<213> Homo sapiens

<400> 533

His His Leu Val Pro Leu Phe Ile Trp Thr Pro Trp Arg Lys Ser Gly

1 5 10 15

Ser Gly Ser Gly Leu Asn Ala Trp Asn Gly Phe Ser Asn Leu Val Arg

20 25 30

Leu Met Gly Pro Trp His Leu Cys Met Gly Leu Lys Ser Trp Ile Ser

35 40 45

118

Val Pro Cys Pro Asn Leu Leu Pro Ala Pro Ser Ala Leu Val Leu Leu

50 55 60

Ala Pro Ala Thr Pro Pro Phe Gly Leu Ile Pro Arg Leu Pro Thr Gly

65 70 75 80

Leu Tyr Cys Phe Pro Ser Ser Asp

85

<210> 534

<211> 66

<212> PROTEIN

<213> Homo sapiens

<400> 534

Leu Lys Arg Gln Met Gly Ser Ser Leu Leu Val Ser Met Lys Asn Pro

1 5 10 15

Tyr His Leu Leu Gly Pro His Ser Leu Met Val Thr Ile Lys Trp Ser

20 25 30

Ser Arg Lys Arg Tyr Cys Ile Leu Gln Val Ser His Tyr Met Met Lys

35 40 45

Lys Gly Lys Arg Asp Lys Leu Pro Phe Leu Leu Leu Ile Asn Phe His

50 55 60

Ser Ala

65

<210> 535

<211> 63

<212> PROTEIN

<213> Homo sapiens

<400> 535

Ile Phe His Ser Gly Met Leu Pro Leu Glu Ile Ala Leu Thr Ile Ser

1 5 10 15

Pro Phe Ser Thr Val Cys Arg Glu Ser Glu Arg Asp Tyr Asn Met Asp

20 25 30

Phe Leu Thr Leu Arg His Leu Met Trp Met Asn Met Asn Ile Met Ser

35 40 45

Glu Leu Lys Met Val Thr Ser Thr Gly Leu Phe Gln Glu Asn Phe

50 55 60

<210> 536

<211> 115

<212> PROTEIN

<213> Homo sapiens

<400> 536

Pro Met Phe Tyr Val Gly Pro Leu Met Cys Leu Leu Thr Gly Val Phe

1 5 10 15

119

Val Ile Ser Gly Ser His Leu Phe Cys Thr Ala Pro Ala Met Ser Asp

20 25 30

Ser Ser Pro Ser Val Thr Cys Cys Arg Val Gln Pro Ser Trp Trp Leu

35 40 45

Ser Pro Thr Ala Gly Phe Trp Arg Pro Trp Pro Ala Pro Cys Thr Leu

50 55 60

Ser Ser Ser Ala Thr Cys Cys Ser Leu Met Trp Leu Leu Ser Ser His

65 70 75 80

Phe Gly Ser Cys Cys Ile Ser Ser Phe Thr Met Gly Leu Ser Val Lys

85 90 95

Cys Arg Tyr Thr Pro Gly Leu Cys Leu Ser Gln Pro Phe Ser Ser Leu

100 105 110

Phe Ser Pro

115

<210> 537

<211> 105

<212> PROTEIN

<213> Homo sapiens

<400> 537

Gly Ser Leu Leu Ser Pro Pro Glu Arg Arg Cys Gly Ser Arg Leu Trp

1 5 10 15

Arg Ala Trp Arg Arg Phe Pro Leu Leu Met Met Trp Arg Thr Pro Met

20 25 30

Thr Thr Arg Leu Arg Thr Gln Ala Cys Arg Arg Asn Ser Ser Arg Pro

35 40 45

Arg Pro Ala Gly Arg Ala Pro Arg Ser Leu Ala Thr Arg Pro Trp Pro

50 55 60

Arg Arg Thr Gly Gly Cys Leu Leu Trp Arg Gly Arg Cys Ser His Arg

65 70 75 80

Arg Gly Gly Cys Pro Trp Cys Pro Arg Lys Pro Arg Ser Trp Pro Arg

85 90 95

Ser Ala Cys Glu Pro Gly Arg Ser Pro

100 105

<210> 538

<211> 140

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

120

<400> 538

Ser Arg Ala Ser Trp Val Pro Pro Gln Pro Cys Arg Cys Ser Met Gly

1 5 10 15

Gln Pro Cys Pro Arg Arg Cys Cys Ser Ser Pro Cys Arg Pro Arg Ala

20 25 30

Arg Arg Ser Pro Ala Ser Trp Glu Thr His Pro Trp Ala Pro Ser Ser

35 40 45

Leu Gly Pro Ser Gln Pro Thr Pro Ser Ser Gly Ser Cys Leu Gln Glu

50 55 60

Gly Ala Cys Pro Arg Ser Cys Arg Pro Gly Glu Gly Ser His His Pro

65 70 75 80

Cys Cys His Pro Cys Leu Ala Leu Leu Gly Val Thr Gly Arg Pro Trp

85 90 95

Ala Trp Val Pro Gln Arg Leu Trp Gly Ser Glu Ala Leu Ala Ser Glu

100 105 110

Ala Ser Arg Lys Thr Val Gly Leu Cys Arg Arg Pro Leu Gly Ser His

115 120 125

Cys Trp Gly Ser Pro Pro Arg Thr Thr Gly Phe Pro

130 135 140

<210> 539

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 539

Ala Ala Leu Thr Ser Ser Ala Ile Ser Gly Pro Gly Trp Thr Pro Arg

1 5 10 15

Pro Pro Thr Phe Leu Ser Pro Trp Pro Ser Pro Ala Ile Pro Leu Leu

20 25 30

Thr Pro Arg Met Ser Trp Cys Ser Ser Leu Thr Thr Trp Pro Asn Ser

35 40 45

<210> 540

<211> 80

<212> PROTEIN

<213> Homo sapiens

<400> 540

Leu Cys Ser Pro Pro Ser Pro Gln Asn Arg Ser Arg Thr Asn Gly Ser

1 5 10 15

Val Thr Thr Ser Ala Thr Glu Ala Trp Cys Arg Thr Thr Leu Pro Ala

20 25 30

Ser Gln Arg Ser Ser Ala Cys Thr Arg Ser Thr Leu Met Ser Cys Thr

121

35 40 45

Glu Ala Ser Arg Asp Pro Ala Lys Met Arg Arg Arg Ser Trp Gln Arg

50 55 60

Arg Arg Leu Pro Ser Val Ile Pro Thr Arg Thr Ala Arg Trp Pro Arg

65 70 75 80

<210> 541

<211> 98

<212> PROTEIN

<213> Homo sapiens

<400> 541

Leu Val Asn Leu Lys Lys Leu Trp Glu Arg Lys Lys Asn Gln Ser Gln

1 5 10 15

Leu Leu Pro His Ala Leu Leu Glu Glu Glu Val Val Ala Val Glu Asn

20 25 30

Glu Val Ala Arg Lys Met Ile Leu Thr Gly Gly Pro Gly Phe Arg Arg

35 40 45

Val Thr Phe His Gly Thr Thr Arg Ile Ser Gly Cys Ser Ser Ser Gly

50 55 60

Leu Leu Cys Ser Gly Val Glu Ser Cys Phe Thr Cys Cys Ser Arg Asp

65 70 75 80

Pro Gly Glu Lys Ser Leu Gly Arg Thr Leu Ser Ile Thr Ile Phe Gln

85 90 95

Lys Glu

<210> 542

<211> 102

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 542

Val Ser Gly Ser Pro Pro Ala Gly Ser Ser Thr Gln Ala His Ala Ser

1 5 10 15

Val Asp Ser Arg Arg Glu Arg Val Lys Ala Val Pro Ala Leu Ala His

20 25 30

Leu Val Gly Trp Gly Val Leu Ile Ser Leu Trp Arg Thr Asn Asp Arg

35 40 45

Arg Val His Ile Asn Ser Ile Leu Thr Gln Ser Cys Phe Leu Asp Phe

50 55 60

Gly Val Glu Ala Phe Tyr Arg Lys Glu Ser Ser Asp Val Ile Leu Ala

122

65 70 75 80

Phe Leu Glu Ala Lys Asp Ser Gly Ser Pro Glu Val Arg Gly Ser Arg

85 90 95

Pro Ala Trp Pro Thr Trp

100

<210> 543

<211> 87

<212> PROTEIN

<213> Homo sapiens

<400> 543

Tyr Ile Gln Val Lys Lys Leu Ile Ser Val Lys Asn Val Glu Asn Val

1 5 10 15

Leu Val Val Gly Ile Ile Ser Leu Phe Ile Thr Lys Ala Tyr Thr Leu

20 25 30

Glu Arg Lys Cys Gly Lys Asn Ile Lys Gln His Phe Ile Asn Val Met

35 40 45

Phe Val Arg Lys Phe Leu Lys Ala Asn Gln Val Trp Lys Cys Ile Phe

50 55 60

Glu Arg Ile Gln Val Lys Asn His Thr Ser Val Lys Phe Ala Ile Ser

65 70 75 80

Leu Leu Glu Leu Arg Lys His

85

<210> 544

<211> 50

<212> PROTEIN

<213> Homo sapiens

<400> 544

Gln Gly Gln Ala Arg Asp Leu Arg Cys Pro Leu Thr Ala Pro Leu Ala

1 5 10 15

Cys Leu His Leu Pro Ser Pro Pro Val Arg Ser Thr Val Cys Ala Met

20 25 30

Asp Ser Leu Arg Pro Val Arg Leu Gln Lys Tyr Pro Gln Ala Ala Ala

35 40 45

ValPro

50

<210> 545

<211> 93

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

123

<400> 545

Ala Ser Leu Leu Ser Arg Ser Ser Met Ser Leu Val Arg Thr Trp Ser

1 5 10 15

Ser Lys Leu Asn Arg His Gln Ser Leu Ala Leu Pro Val Pro Gln Gly

20 25 30

Pro Ser Gly Ala Leu Trp Lys Asn Leu Arg Lys Pro Pro Gly Val Thr

35 40 45

Lys Gly Pro Gly Lys Val Lys Val Ser Pro Leu Gly Asp Ser Cys Ser

50 55 60

Pro Arg Gly Gln Gln Glu Pro Gly Gly Pro Ala Val Trp Lys Ile Leu

65 70 75 80

Phe Trp Ser Ala Arg Arg Ser Leu Val Ser Pro Gly Trp

85 90

<210> 546

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 546

Arg Arg Trp Pro Arg Pro Arg Arg Ser Ala Val Ser Cys Phe Arg Ile

1 5 10 15

Thr Arg Gly Pro Arg Ala Ala Ser Pro Glu Thr Ser Thr Arg Pro Pro

20 25 30

Val Leu Pro Ala Ser Pro Arg Thr Ser Pro Trp Ser Pro

35 40 45

<210> 547

<211> 60

<212> PROTEIN

<213> Homo sapiens

<400> 547

Ser Arg Ser Ser Ala Thr Leu Pro Gly Arg Arg Arg Arg Arg Trp Leu

1 5 10 15

Arg Arg Arg Arg Gln Pro Ile Ser Val Ala Pro Ala Gly Pro Pro Arg

20 25 30

Arg Pro Asn Gln Lys Pro Asn Pro Pro Gly Gly Ala Arg Cys Val Ile

35 40 45

Met Arg Pro Thr Trp Pro Gly Thr Ser Ala Phe Thr

50 55 60

<210> 548

<211> 30

<212> PROTEIN

<213> Homo sapiens

124

<400> 548

Arg Glu Ser Arg Ala Met Ile Ser Leu Gly Ala Leu Arg Glu Asp Trp

1 5 10 15

Asn Gln Lys Arg Ser Leu Gly Gln Gln Ile Pro Val Val Ile

20 25 30

<210> 549

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 549

Arg Ser Ile Asn Thr Glu Val Asn Ile Arg Asn Ile Asn Ile Pro Gln

1 5 10 15

Lys Lys Thr Arg Ile Lys Asn Ile Asn Ile Ser Ile Asn Ile Arg Asn

20 25 30

Thr Lys Glu Lys Arg Leu Leu Met Leu Leu Ile Lys Arg Val Cys Leu

35 40 45

Gln Gln Lys Glu Leu Asn Leu Met Ile

50 55

<210> 550

<211> 94

<212> PROTEIN

<213> Homo sapiens

<400> 550

Ala Ser Val Pro Gly Ser Gly Pro Ser Thr Gln Thr Pro Leu Thr Phe

1 5 10 15

Thr Pro Ala Leu Cys Ser Leu Arg Arg Leu Arg Pro Ser Met Ser Ser

20 25 30

Thr Gly Arg His Thr Pro Cys Glu His Arg Thr Gln Ala Thr Leu Ala

35 40 45

Cys Pro Gly Ala Arg Arg Gln Leu Pro Arg Asn Phe Leu Gly Glu Arg

50 55 60

Ala Leu Gly Leu Pro Ser Pro Leu Arg Val Ser Lys Trp Asp Asp Ser

65 70 75 80

Arg Pro Arg Lys Thr Arg Gly Ser Leu Gly Leu Pro Glu Leu

85 90

<210> 551

<211> 76

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 551

125

Trp Ser Pro Pro Gln Pro Arg Pro Pro Arg Asp Ala Ala Gly Phe Ser

1 5 10 15

Val Ser Val Arg Asp Ser Arg Glu Glu Pro Gly Gly Ser Ser Pro Arg

20 25 30

Gly Ala Met Leu Arg Ala Gly Leu Ser Ser Val Thr Ile Pro Ala Cys

35 40 45

Asn Ile Ser Val Asn Ser Glu Met Asp Leu Gly Pro Leu Thr Pro Arg

50 55 60

Thr Gln Pro Trp Ala Arg Ala Arg Gly Arg Arg Lys

65 70 75

<210> 552

<211> 39

<212> PROTEIN

<213> Homo sapiens

<400> 552

Gln Pro Pro Lys Ala Leu Gln Pro Pro Arg Ala Pro Gln Leu Pro Asn

1 5 10 15

Ala Pro Gln Pro Pro Ser Ile Pro Gln Pro Pro Arg Ala Pro Gln Pro

20 25 30

Leu Lys Leu Leu Gln Cys Leu

35

<210> 553

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 553

Gly Leu Thr Met Ser Pro Leu Trp Pro Ser Cys Pro Trp Thr Gly Cys

1 5 10 15

Arg Cys Trp Lys Pro Cys Ala Gly Thr Gly Leu Ala Pro

20 25

<210> 554

<211> 45

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 554

Thr Leu Glu Ser Ala Leu Glu Lys Gly Phe Met Asn Leu Ala Asn Val

1 5 10 15

Gly Lys Pro Ala Ala Val Ser Ala Pro Leu Phe Ser Cys Lys Glu Ser

20 25 30

126

Ala Val Asn Val Gly Asn Leu Leu Ala Lys Pro Leu Ile

35 40 45

<210> 555

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 555

Leu Leu Lys Asn Ser Leu Gln Arg Leu Phe Val Leu Glu Leu Gly Val

1 5 10 15

Ser Leu Ile Gln Met Thr Cys Arg Gly Trp His Thr Phe Trp Ser Thr

20 25 30

Trp Tyr Ser Trp Val Val

35

<210> 556

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 556

Leu Leu Lys Asn Ser Asn Cys Arg Ala Cys Phe Cys Cys Gly Gln Ser

1 5 10 15

<210> 557

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 557

His Leu Leu Leu Ser Leu His Pro Ser Leu Pro Lys Leu Pro Gln Asn

1 5 10 15

ProLeu

<210> 558

<211> 51

<212> PROTEIN

<213> Homo sapiens

<400> 558

Arg Glu Ile Arg Cys Val Gln Ala Val Val Ile Leu Cys Ser Glu Leu

1 5 10 15

Ser Ser Val His Glu Val Leu Ser Ser Cys Val Ala Leu Phe Lys Gly

20 25 30

Ala Arg Glu His Ile Cys Leu Arg Glu Thr Tyr Arg Leu Ile Ser Thr

35 40 45

Ile Ala Ile

50

<210> 559

127

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 559

Asn Val Lys Asn Pro Lys Val Ile Leu Ile Phe Leu Met Lys Lys Gln

1 5 10 15

Pro Ser Lys Val Arg Arg Lys Glu Ala His Gly Leu Pro Leu

20 25 30

<210> 560

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 560

Thr Arg Thr Arg Glu Val Ser Pro Arg His Ser Thr Leu Thr Ala Ser

1 5 10 15

Arg Arg Ser Ser Thr Ala Ser Thr Ser Trp Glu Gly Trp Pro Pro Gly

20 25 30

<210> 561

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 561

Ile Cys Pro Trp Lys Arg Gln Pro Gly Ala Val Ala Gln Arg Ile Gly

1 5 10 15

Ser His Leu Val Pro Thr Ala Thr Leu Phe Leu Leu Asn Gln His Leu

20 25 30

Gly Lys Thr Val Arg Arg Thr Val Leu Glu Trp Arg Leu Thr Cys Trp

35 40 45

<210> 562

<211> 36

<212> PROTEIN

<213> Homo sapiens

<400> 562

Gln Thr Leu Arg Pro Trp Leu Trp Leu Thr Ala Trp Ala Ala Ser Thr

1 5 10 15

Gly Ala Cys Gly Arg Arg Gln Leu Gly Ala Asn Ala Arg Cys Ser Ser

20 25 30

Gly Met Arg Cys

35

<210> 563

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 563

128

Arg Arg Thr Lys Pro Ala Ala Leu Ser Pro Val Met Val Pro Arg Gln

1 5 10 15

Lys Tyr Gln Gln Arg Val Lys Arg Ser Ser Ala Asp Pro Leu Pro Leu

20 25 30

Cys

<210> 564

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 564

Arg Leu Val Arg Gly Arg Arg Leu Arg Thr Ala Glu Asn Val Lys Asn

1 5 10 15

Asn

<210> 565

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 565

Leu Asp Pro Met Ala Pro His Arg Ser Pro Thr Asn Lys Leu Leu Pro

1 5 10 15

Gln

<210> 566

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 566

Asn Ser Lys Lys Pro Pro Gln Ser Lys Arg Thr Asn Gly Met Lys Met

1 5 10 15

Asn Met Lys Gly Pro Glu Gly Arg Arg

20 25

<210> 567

<211> 56

<212> PROTEIN

<213> Homo sapiens

<400> 567

Leu Pro Val Asn Val His Leu Val Ser His Ser Ile Thr Leu Leu Val

1 5 10 15

Ser Thr Thr Thr Asn Val Gly Leu Asn Leu Arg Phe Val Glu Gln Arg

20 25 30

129

Glu Ser Val Lys Thr Leu Gln Ala Val Ser Ala Val Asn Ala Lys Glu

35 40 45

Gly Ser Leu Leu Met Pro Pro Asp

50 55

<210> 568

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 568

Ser Arg Gly Gln Arg Ser Leu Val Ser Ala Asp Glu Ser Thr Thr His

1 5 10 15

Leu Arg Asn Ala Arg Lys Thr Lys

20

<210> 569

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 569

Phe Val Lys Trp Leu Lys Lys Glu Glu Lys Glu Val Gln Leu Gln Asp

1 5 10 15

<210> 570

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 570

Gly Leu Gln Thr Lys Arg Thr Leu Arg Ile Arg Lys Pro Arg Val Leu

1 5 10 15

Lys Pro Leu Gln Phe Arg Leu Ala Ser Gln Ile Leu Pro Thr Leu Ser

20 25 30

Lys

<210> 571

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 571

Gln Thr Glu Ile Val Ser Gly Ile Leu Ala Tyr Cys

1 5 10

<210> 572

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 572

Val Ala Thr Pro Gly Arg Pro Trp Pro Pro Ser Phe Ser Ala Pro Cys

1 5 10 15

130

Cys Trp Arg Leu Trp Pro

20

<210> 573

<211> 33

<212> PROTEIN

<213> Homo sapiens

<400> 573

Ala Ser Ser Pro Ser Leu Leu Val Gly Ala Asn Arg Ser Ser Ala Met

1 5 10 15

Gly Arg Thr Gly Gly Gly Cys Gln Trp Trp His His Phe Trp Val Pro

20 25 30

ile

<210> 574

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 574

Ser Tyr Ala Leu Ser Asn Lys Asn Leu Leu Ile

1 5 10

<210> 575

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 575

Met Ala Leu His Gly Val Gln Leu Arg Lys Ser Lys Asp Leu His Phe

1 5 10 15

Leu Leu Gln Gly Cys Cys Leu Met Leu Lys Arg Val Trp Ile Pro Met

20 25 30

Gln Leu Ile Ala Ala Leu Met Thr Ile Trp Met Asn Ile Pro Leu Glu

35 40 45

Lys Met Trp Gln Leu Thr Val Val Leu Asn Gly Ser Gly Leu

50 55 60

<210> 576

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 576

Leu Lys Pro Ala Ala Pro Arg Gly Pro Ile Arg Ala Lys Thr Ala Ser

1 5 10 15

Ile Phe

131

<210> 577

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 577

Lys Met Leu Lys Arg Thr Lys Cys Phe Thr Phe Gly

1 5 10

<210> 578

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 578

Arg Glu Arg Thr Arg Ile Asn Arg Lys Glu Leu Arg Leu Tyr His Pro

1 5 10 15

Asp Thr His His Ala

20

<210> 579

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 579

Arg Ile Pro Pro His His Pro Arg Cys Leu Arg Asp Ala

1 5 10

<210> 580

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 580

His His His Gln Cys Gln Pro Trp Ser Pro Thr Leu Pro Arg Thr Pro

1 5 10 15

Leu Leu Pro

<210> 581

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 581

Met Gln Lys Lys Lys Ser Ser Arg Glu Ile Arg Lys Arg Arg Arg Met

1 5 10 15

Met Met Met Arg Arg

20

<210> 582

<211> 13

<212> PROTEIN

<213> Homo sapiens

132

<400> 582

Trp Met Arg Arg Leu Glu Glu Arg Trp Glu Asn Lys Leu

1 5 10

<210> 583

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 583

Ala Gln Lys Lys Asn Arg Arg His

15

<210> 584

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 584

Arg Ser Glu Arg Lys Trp Ser Trp Met Thr Leu Pro Gly Ser Trp Ser

1 5 10 15

Met Arg Arg Thr

20

<210> 585

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 585

Ser Leu Lys Lys Gln Tyr Pro Lys

15

<210> 586

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 586

Lys Leu Phe Leu Val Arg Glu Val Gln Arg Gly Glu Lys Gln Glu Leu

1 5 10 15

Arg Arg Ile Lys Gln

20

<210> 587

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 587

Leu Val Ala Ile Ser Phe Lys Thr Val Phe Lys Ala Ser His Ala Ala

1 5 10 15

Lys Thr

133

<210> 588

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 588

Glu Leu Val Ser Thr Gly Ile Met Thr Phe His Leu Leu Ser Val Asp

1 5 10 15

Ile Tyr Gln Glu Ala Thr

20

<210> 589

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 589

Asn Ser Asp Gln Asn Leu Phe Thr Glu Ile Ile Leu Asn Pro Pro Lys

1 5 10 15

His Gln

<210> 590

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 590

Ser Arg Lys Arg Ala Val Ser Asn Ser Arg

1 5 10

<210> 591

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 591

Met Ala Pro Ser Ser Ala Ala Ala Asp Gln Asp Ser Gly Cys Trp Met

1 5 10 15

Thr Trp

<210> 592

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 592

His Asn Gly Tyr Val Leu Gln Lys Lys Leu His Phe Thr Phe Tyr Thr

1 5 10 15

Cys Leu

<210> 593

<211> 13

134

<212> PROTEIN

<213> Homo sapiens

<400> 593

Gln Pro Pro Pro Arg Ser Glu Val Leu Ser Cys Pro Leu

1 5 10

<210> 594

<211> 13

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 594

Arg Ala Leu Ala Ala Leu Pro Ser Ser Ser Thr Leu Arg

1 5 10

<210> 595

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 595

Asp His Lys Lys Met Tyr Ser Ala His Ser Cys

1 5 10

<210> 596

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 596

Ser Ser Lys Lys Ile Ser Lys Met Ile Phe Val

1 5 10

<210> 597

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 597

Gln Ser His Arg Pro Pro Arg Ala Ser Ser Ala Gly Asp Leu Gln Cys

1 5 10 15

Gln Ala

<210> 598

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 598

Ser Val Gly Cys Leu Gly Cys Pro Gly Ser Arg Leu Pro Arg Ser Ala

1 5 10 15

Pro Ser Pro Met Ser Met Arg

20

135

<210> 599

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 599

Ser Thr Val Arg Glu Ala Leu Ser Pro Val Gln His Gly Lys Cys Arg

1 5 10 15

Lys Arg Arg Asn

20

<210> 600

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 600

Ala Ala Lys Phe Ala Arg Cys Ala Thr Ala Leu Glu His Val Ser Ser

1 5 10 15

Ser Leu Arg Leu Arg Thr Arg Thr Arg Thr

20 25

<210> 601

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 601

Thr Ser Ala Leu Asp Val Ser Ala Pro Ala Ala Met Ile Ala Ala Cys

1 5 10 15

Ser Ala Ala Leu Leu Ser Ala Trp Ala Arg Trp Asn Val Cys

20 25 30

<210> 602

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 602

Gln Asp Arg Ile Phe Arg Val Phe Gln Asn His

1 5 10

<210> 603

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 603

Glu Thr Asp Thr Ser Ser Ala Phe Ser Ser Leu Leu Pro Leu Arg Asn

1 5 10 15

Leu Ser Gln Leu

20

136

<210> 604

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 604

Met Glu Lys Thr Gln Gln Met Gly Arg Arg Met Glu His Ile Leu

1 5 10 15

<210> 605

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 605

Arg Lys Leu Phe Ser Ser Lys Arg Lys Lys Lys Lys

1 5 10

<210> 606

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 606

Ala Asp Lys Lys His Val Gln Ser Val Thr Ser Val Pro

1 5 10

<210> 607

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 607

Arg Arg Lys Asn Gln Arg Cys Leu Thr Phe Gln Pro Phe Thr

1 5 10

<210> 608

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 608

Lys Thr Val Phe Leu Asn His Trp Arg Gly Ser

1 5 10

<210> 609

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 609

Lys Lys Lys Lys Asn Met Lys Pro Ser Leu Leu Leu

1 5 10

<210> 610

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 610

137

Cys Gly Pro Pro Ala Trp Ala Leu Cys

15

<210> 611

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 611

Arg Arg Tyr Phe Trp Lys Arg Gln Glu Met

1 5 10

<210> 612

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 612

Asp Gly Trp Gly Ala Thr Ala Pro Thr Arg Gly

1 5 10

<210> 613

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 613

Arg Gly Gly Ile Leu Leu Ser Met Phe Leu

1 5 10

<210> 614

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 614

Gln Glu Ser Pro Arg Ser Pro Leu Leu

15

<210> 615

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 615

Lys Arg Lys Lys Ala Ala Arg Lys Leu Thr

1 5 10

<210> 616

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 616

Thr His Phe Phe Thr Phe Phe Asn Leu

15

<210> 617

<211> 11

<212> PROTEIN

138

<213> Homo sapiens

<400> 617

Glu Val Val Phe Trp Met Thr Ser Gly Leu Ile

1 5 10

<210> 618

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 618

Met Arg Lys Leu Ala Phe Pro Gln Phe Ser Ser Cys

1 5 10

<210> 619

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 619

Val Ser Lys Lys Asp Leu Ser Pro

15

<210> 620

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 620

Ser Ile Gly Phe Leu Leu Gln Asn Leu

15

<210> 621

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 621

Glu Val Lys Lys Pro Pro Val Cys Ser Arg

1 5 10

<210> 622

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 622

Leu Leu Phe Ile Pro Thr Gly Ile Ser Leu Val Glu Thr Ala Arg Glu

1 5 10 15

Gln Leu Phe Arg Gly

20

<210> 623

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 623

139

Ala Val Pro Pro His Val Lys Arg His Ala Val Asn Leu

1 5 10

<210> 624

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 624

Thr Cys Ser Pro Gln Ser Leu Leu Ser Ser Trp Arg Ser Thr Gln Pro

1 5 10 15

His Val Pro Pro Ser Thr Leu Trp Gly Val Arg Met Thr Ser Leu Ser

20 25 30

Ala Ser Thr Ser Leu Trp

35

<210> 625

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 625

Val Asn Leu Phe Leu Thr Lys Met Arg Met

1 5 10

<210> 626

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 626

Ala Leu Lys Lys Arg Arg Met Val Leu Leu Lys Ser

1 5 10

<210> 627

<211> 233

<212> PROTEIN

<213> Homo sapiens

<400> 627

Trp Leu Ala Pro Pro Glu Ala Gly Ala Ala Arg Gly Ala Glu Ala Ala

1 5 10 15

Ala Val Val Thr Ala Ala Phe Thr Lys Glu Ala Ser Lys Lys Ala Met

20 25 30

Gly Pro Leu Asp Glu Ala Pro Gly Lys Ser Ser Ala Ala Arg Ala Gly

35 40 45

Met Thr Glu Ala Arg Gly Gly Leu Arg Ser Gln Gln Gly Gly Met Glu

50 55 60

His Asp Val Ala Leu Arg Arg Glu Gly Leu Ala Gln Gly Arg Ser Thr

65 70 75 80

140

Pro Ala Gln Thr Ala Arg Thr Gly Ala Pro Tyr Gly Arg Asn Arg Arg

85 90 95

Arg Arg Arg Arg Ala Ala Gly Gly Ser Glu Gln Gly Pro Gly Glu Thr

100 105 110

Ala Thr Ala Gly Ala Pro Pro Ala Leu Met Val Val Pro Ala Leu Leu

115 120 125

Ala Gly Gly Asn Met Gly Asn Gly Gly Ala Ser Leu Asn Leu Ile Cys

130 135 140

Glu Gly Ile Glu Glu Gly Val Val Lys Arg Arg Gly Gly Glu Gly Glu

145 150 155 160

Ala Ala Leu Thr Cys Gly Gly Ala Glu Arg Leu Lys Ala Leu Arg Arg

165 170 175

Thr Arg Met Gly Ser Gln Ser Gly Ala Trp Thr Met Arg Met Lys Lys

180 185 190

Trp Ala Pro Leu Met Pro Leu Gly Pro Ser Cys Leu Ser Arg Arg Ala

195 200 205

Pro Arg Ser Pro Phe Leu Arg Ser Arg Ser Trp Thr Ser Lys Gly Trp

210 215 220

Arg Arg Arg Arg Asn Leu Pro Lys Gly

225 230

<210> 628

<211> 70

<212> PROTEIN

<213> Homo sapiens

<400> 628

Asn Asp Leu Arg Lys Asn Lys Glu Gln Pro Ala Lys Thr Gly Pro Ser

1 5 10 15

Gln Lys Arg Thr Gly Arg Arg Gly Gly Ser Ile Lys Val Leu Ile Pro

20 25 30

Thr Met Glu His Arg Thr Gly Phe Thr Leu Ala Ala Thr Gly Thr Glu

35 40 45

Ile Thr Ser Ile Cys Leu Thr Phe Ile Lys Met His Thr Ser Gln Gly

50 55 60

Val Trp Leu Ile Tyr Lys

65 70

<210> 629

<211> 78

<212> PROTEIN

<213> Homo sapiens

<400> 629

141

Tyr Cys His Leu Arg Leu Pro Ser Gln Ala Pro Leu Lys Gly Lys Arg

1 5 10 15

Ala Gln Asn Gln Ala Gln Pro Asn Leu Leu Pro Gln Asn Gln His His

20 25 30

Leu Leu Arg Gln Trp Thr Gln Thr Val Gln Ala Pro Arg Phe Pro Leu

35 40 45

Leu Lys Ser Arg Ser Ser Trp Ile Gln Pro Leu Trp Ser Gln Glu Leu

50 55 60

Trp Met Pro Ser Gln Trp Arg Val Leu Glu Ile Leu Thr Asn

65 70 75

<210> 630

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 630

His Asn Pro Arg His Gln Thr Ser Val Leu Ser Phe Thr Thr Ala Asn

1 5 10 15

Thr Val Pro Thr Ala Ile Gly Gln Leu Trp Glu Cys Leu Ser Thr Thr

20 25 30

Arg Lys Asp Thr Gln Lys

35

<210> 631

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 631

Met Phe Leu Ile Gln Arg Glu Leu Leu Lys Thr Leu Asn Met Ile Gln

1 5 10 15

Val Leu Met Ser Val Val His

20

<210> 632

<211> 15

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 632

Thr Thr Ser Ser Leu Val Ala Ser Ser Gln Asn Trp Gly Gln Pro

1 5 10 15

<210> 633

<211> 11

<212> PROTEIN

<213> Homo sapiens

142

<400> 633

Met Gly Glu Lys Asn Gln Ser Leu Trp Thr Ser

1 5 10

<210> 634

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 634

Leu Pro Val Tyr Arg Gln Thr His Trp Arg Leu Tyr Pro Lys Pro Phe

1 5 10 15

Ala Gly Leu Phe Pro Leu Lys Pro

20

<210> 635

<211> 97

<212> PROTEIN

<213> Homo sapiens

<400> 635

Gln Pro Ala Arg Gln Ala Arg Ala Gln Asp Arg Trp Arg Thr Gln Arg

1 5 10 15

Pro Gly Pro Ala Ser Ser Pro Gly Ser Arg Ser Pro Ser His Val Arg

20 25 30

His Leu His His Gly Trp Ala Ser Val Pro Gly Leu Pro Lys Val Ala

35 40 45

Met Ala Pro Gly Thr Pro Gly Ile Arg Gly Pro His Ala Pro Pro Thr

50 55 60

Trp Leu Pro Ala Arg Pro Ser Arg Leu Pro Gly Ser Ala Ala Ala Pro

65 70 75 80

Thr Leu Pro Arg Ala Leu Ala Arg Leu Pro Pro Ala Val Gly Gly Cys

85 90 95

Val

<210> 636

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 636

Arg Ser Val Lys Arg Cys Lys Arg Ser Leu Pro Lys Gly Ile Val Lys

1 5 10 15

Leu Thr Leu Gln Ile Tyr Asn Ile

20

<210> 637

143

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 637

Gly Asn Phe Gln Arg Thr Thr Cys His Ser Ile Leu Ser Ser

1 5 10

<210> 638

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 638

Leu Lys Pro Tyr Arg Ser Val Leu Val Leu Ile Lys Met Phe Phe

1 5 10 15

<210> 639

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 639

Asp Glu Lys Lys Arg Lys Gln Lys

15

<210> 640

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 640

Phe Gly Arg Ser Leu Asn Leu Thr Val Lys Trp Leu Val Thr Ser Gly

1 5 10 15

Lys Arg Val Thr Ser Ile Arg

20

<210> 641

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 641

Ser Trp Cys Leu Cys Cys Arg Leu Ile Ala Met Lys Glu Met Thr Trp

1 5 10 15

Thr Lys Ile

<210> 642

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 642

Lys Pro Pro Pro Thr His Pro Asp Arg Gly Val Glu

1 5 10

144

<210> 643

<211> 82

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 643

Val Thr Pro Pro Val Ala Arg Met Gly Arg Ser Pro Thr Ser Lys Pro

1 5 10 15

Trp Gly Met Arg Arg Ala Glu Thr Gln Thr Ala Ala Pro Arg His Leu

20 25 30

Pro Lys Gln Ser Pro Asp Ala Ser Pro Pro Val Ala Pro Pro Ala Trp

35 40 45

Thr Arg Pro Gly Ser Thr Ala Val Ser His Pro Gly Ser Thr Pro Gly

50 55 60

Ala Pro Ser Leu Ala Thr Ala Val Pro Ser Pro Leu Asp Ser Ser Ala

65 70 75 80

Met Ser

<210> 644

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 644

Ala Asn Pro Gln Leu Leu Cys Gln Asn Pro Glu Lys Leu Ala Leu Lys

1 5 10 15

Glu Asp Leu Leu Tyr Leu Arg Thr Ser Gly Glu Pro Trp Asp Thr Glu

20 25 30

Arg Asn Leu Arg Pro Leu Ser Leu Gln Cys Gln Ser Leu Ala Leu Pro

35 40 45

<210> 645

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 645

Asn Pro His Phe Arg Pro Pro Arg Thr Trp Arg Ala Thr Thr Ser Ala

1 5 10 15

Gln Leu Ala Arg Cys Ser Pro Trp Ala Thr Leu Pro His Met Pro Arg

20 25 30

Ala Arg Ser Ser Thr Ala Ala Ala

35 40

<210> 646

145

<211> 37

<212> PROTEIN

<213> Homo sapiens

<400> 646

Thr Thr Ala Gln Thr Ala Pro Pro Ser Thr Arg Met Met Ala Thr Cys

1 5 10 15

Pro Cys Pro Gln Gly Trp Pro Gln Cys Pro Val Ala Glu Arg Ala Val

20 25 30

Glu Thr Ile Cys Pro

35

<210> 647

<211> 41

<212> PROTEIN

<213> Homo sapiens

<400> 647

His Pro Leu Pro Thr Leu Thr Cys Pro Thr Met Pro Gly Arg Thr Pro

1 5 10 15

Thr Ala Asn Pro Pro Thr Pro Ser Ala Ala Ser Tyr Leu Trp Pro Ser

20 25 30

Arg Thr Leu Gln Pro Ser Ala Cys Gln

35 40

<210> 648

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 648

Glu Ala Thr Ser Asn Gln Asp Leu Leu Gln Met Val Pro Cys Ser Met

1 5 10 15

Gln Asp Pro Leu Leu Ser Leu Trp Thr Leu Phe Thr Glu

20 25

<210> 649

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 649

Asn Val Asn Val Cys Ser Pro Lys Ile Leu Ile Leu Lys His Gln Lys

1 5 10 15

Met Ile Arg Asp Phe Arg Arg Asn Leu Arg Lys Trp Leu Lys Ser Tyr

20 25 30

Lys Gly Lys Lys Gln Ile

35

<210> 650

<211> 36

146

<212> PROTEIN

<213> Homo sapiens

<400> 650

Ala Asn Gln Glu Ile Ser Ile Val Lys Thr Ile Val Val Cys Leu Lys

1 5 10 15

Val Gly Cys Val Ile Leu Lys Met Thr Val Val Met Ala Ala Met Lys

20 25 30

Lys Ile Ala Gln

35

<210> 651

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 651

Ala Ala Pro Met Met Ser Ser Met Ala Cys Gly Ser Ala Glu Ser Ser

1 5 10 15

<210> 652

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 652

Arg Leu Phe Trp Met Arg Arg Leu Trp Cys Phe Arg Gln Leu Ser Gly

1 5 10 15

Asp Ile Ser Arg Gly Gln Ser Ser

20

<210> 653

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 653

Glu Gln Lys Lys Ile Ser Pro Phe Ile Tyr Phe Phe Leu

1 5 10

<210> 654

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 654

Lys Leu Lys Lys Ala Ser Val Leu Leu Phe Ser

1 5 10

<210> 655

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 655

Asp Thr Gln Ile Pro Arg Thr Ser Arg Trp Cys

147

1 5 10

<210> 656

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 656

Ser Val Lys Lys Arg Met Ile Ile Phe

15

<210> 657

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 657

Arg Val Pro Phe Leu Phe Val Val Gln Val

1 5 10

<210> 658

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 658

Leu Glu Val Gly Ala Thr Ser Ser

15

<210> 659

<211> 94

<212> PROTEIN

<213> Homo sapiens

<400> 659

Ser Arg Ser Pro Lys Ser Met Ser Pro Pro Gly Arg Thr Gln Leu Pro

1 5 10 15

Pro Ser Gln Leu Pro Gln Pro His Arg His Arg Lys Thr Cys Ser Arg

20 25 30

Arg Ala Thr Pro Leu Ser Ser Leu Ala Ala Ala Pro Gly Ala Arg Ala

35 40 45

Gly Ala Arg His Pro Pro Pro Thr Arg Arg Gln Arg Ser Leu Cys Ser

50 55 60

Ser Gln Pro Ser Gln Pro Arg Pro Arg Ser Cys Leu Gly Thr Pro Leu

65 70 75 80

Ala Gly Leu Pro Ala Cys Pro Ser Pro Cys Pro Pro Val Arg

85 90

<210> 660

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 660

148

Arg His Pro Pro Pro Thr Arg Arg Pro Ser Gln Pro Arg Pro Arg Ser

1 5 10 15

<210> 661

<211> 70

<212> PROTEIN

<213> Homo sapiens

<400> 661

Pro Leu Ser Gly Thr Trp Gly Thr Leu Arg Ala Pro Ser Ser Phe His

1 5 10 15

Pro Leu Val Ala Thr Gln Ala Leu Leu Ser Gln Arg Gly His Pro Arg

20 25 30

Thr Ala Leu Gly Gly Ser Gln Pro Leu Ser Pro Arg Arg Ala Ala Ala

35 40 45

Pro Thr Gln Gln Leu Pro His Ala Leu Leu Ala Pro Ser Leu Gln Asp

50 55 60

Leu Ala Lys Arg Pro Pro

65 70

<210> 662

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 662

Arg Ala Trp Ala Pro Ala Pro Pro Thr Arg Cys Val Trp Gln Gln Pro

1 5 10 15

Ser

<210> 663

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 663

Thr Arg Lys Ala Ser Ala Ala Ser Cys Ala Ser Gly Val Thr Thr Ser

1 5 10 15

Thr His Arg Pro Ala Ser Ala Trp Thr Ser Met His Gln Thr

20 25 30

<210> 664

<211> 36

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 664

Val Leu Thr Val Ala Cys Arg Ser Ala Gln Pro Pro Arg Pro Trp Asp

149

1 5 10 15

His Ala Ala Pro Val Thr Cys Thr Cys Gly Arg Thr Arg Gly Leu Gly

20 25 30

Gly Gly Thr Thr

35

<210> 665

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 665

Lys Thr Lys Asn Leu Pro Leu Lys Thr Lys Leu Lys Lys Lys Glu Asn

1 5 10 15

Lys Thr Thr Leu Asn Leu Gln Met Ala Glu His His Leu Leu Cys Pro

20 25 30

Arg Ile Met Asn Lys Ala Gln Pro Tyr Gly Ile Cys

35 40

<210> 666

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 666

His Pro Ile His Ile Gly Cys Thr Gln Thr Met Val Met Gly Ile Ile

1 5 10 15

Phe Met Cys Leu Arg Leu Cys Pro His Ile Leu Asp Arg Leu Gln Arg

20 25 30

Gly Leu Pro Gly Asn Trp Glu Leu Lys Leu Glu

35 40

<210> 667

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 667

Val Ser Ser Thr His Ser Thr Thr Thr Gly Ser His Trp Lys Gln Pro

1 5 10 15

Thr Asp Gly Ala Val Cys Thr Arg Thr Ser Met Arg Ser Phe Pro Thr

20 25 30

Arg Ser Thr

35

<210> 668

<211> 27

<212> PROTEIN

<213> Homo sapiens

150

<400> 668

His Thr Trp Asn Gly Pro Arg Ile Gly Val Asn Val Gly Phe Ser Gly

1 5 10 15

Gln Thr Phe Glu Leu Leu Leu Met Leu Met Tyr

20 25

<210> 669

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 669

Arg Ile Lys Pro Phe Ser Thr Asn Ser Val Leu Arg Arg Thr Gly Ser

1 5 10 15

Ser Ile Glu Glu Leu

20

<210> 670

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 670

Val Thr Trp Met Lys Arg Arg Arg Arg Asn Leu Ile Leu Arg Lys Pro

1 5 10 15

Glu Trp Ile Phe Lys

20

<210> 671

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 671

Leu Glu Lys Lys Glu Ser Glu Lys Trp Met

1 5 10

<210> 672

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 672

Val Ser Asn Trp Arg Val Lys Ser Leu Pro Cys Leu Pro Phe Phe Ser

1 5 10 15

Val Leu

<210> 673

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 673

151

Gly Cys Thr Pro Gln Leu Cys Trp Thr

15

<210> 674

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 674

Thr Phe Leu Leu Trp Lys Asn Trp Lys Arg Ala Leu Ser Thr Arg Lys

1 5 10 15

Ile Cys Ser Lys

20

<210> 675

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 675

Glu Glu Lys Lys Thr Glu His Tyr Ala Tyr

1 5 10

<210> 676

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 676

Phe Leu Gln Gly Val Ala Leu Ala Val Gln Ala Ser

1 5 10

<210> 677

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 677

Leu Met Phe Phe Cys Gly His Gln Asn

15

<210> 678

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 678

Ile Arg Lys Lys Ala Asp Leu His Thr Gln

1 5 10

<210> 679

<211> 48

<212> PROTEIN

<213> Homo sapiens

<400> 679

Gly Ala Pro Arg Ser Pro Ser Ser Val Ala Gly Ser Ser Ser Ala Thr

1 5 10 15

152

Trp Gln Ser Gly Ala Met Trp Val Gln Pro Pro Trp Glu Gln Leu Pro

20 25 30

Gly Gly Ser Cys Thr Leu Arg Met Gly Leu Met Ser Thr Thr Ala Ser

35 40 45

<210> 680

<211> 126

<212> PROTEIN

<213> Homo sapiens

<400> 680

Ile Arg Thr Pro Gly His Gly Ser Pro Pro Ala Arg Trp Glu Ser Pro

1 5 10 15

Trp Pro Val His Thr Arg Pro Arg Ser Ser Pro Gly Met Asn Ser Glu

20 25 30

His Ser Gly Ser Ala Gly Val Val Ala Leu Ser Arg Leu Ala Met Val

35 40 45

Gln Ser His Pro Met Ser Leu Ser Leu Trp Pro Gly Pro Ser Arg Gly

50 55 60

His Gln Arg Ser Ser Ser Leu Ala Phe Pro Pro Ala Gly Ala Pro Trp

65 70 75 80

Val Asn Ser Glu Ser Gly Gly Arg Trp Arg Trp Thr Arg Ala Thr Ala

85 90 95

Arg Pro Ser Pro Trp Gly Ser His Thr Ala Pro Ser Leu Gly Leu Ser

100 105 110

Glu Pro Pro Pro Pro Ser Ser Gly Thr Ser Trp Gly Gln Pro

115 120 125

<210> 681

<211> 56

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 681

Thr Leu Trp Thr Leu Cys Ser Ser Thr Pro Thr Cys Leu Arg Thr Cys

1 5 10 15

His Leu Met Arg Ser Leu Trp Pro Pro Gln Arg Pro Ser Asn Ser Arg

20 25 30

Lys Phe Gly Trp Gln Met Arg Ala Ser Thr Pro Ala Pro Cys Leu Thr

35 40 45

His Cys Thr Phe Trp Thr Arg Leu

50 55

153

<210> 682

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 682

Pro Pro Pro Gly Trp Leu Pro Arg Leu Thr Arg Glu Arg Ala Ala Ala

1 5 10 15

Ala Leu Arg Ala Val Gly Thr Gln Ile Ser Ile

20 25

<210> 683

<211> 76

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 683

Ser Ile Lys Lys Lys Lys Asn Lys Glu Gly Val Gly Arg Glu Val Leu

1 5 10 15

Val Val Val Val Pro Val Ala Ile Ala Glu Leu Val Val Leu Val Val

20 25 30

Leu Ser Leu Ala Leu His Thr Val Leu Ala Gln Val Val Leu Leu Lys

35 40 45

Gly Lys Arg Asp Thr Val Gly Val Asp Leu Gln Gln Ser Lys Leu Asp

50 55 60

Val Ala Gly Val Glu Ala Ile Leu Ala Glu Leu Lys

65 70 75

<210> 684

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 684

Leu Gln Asp Gly Ser Leu Asp Arg Asn Leu Gly Ser Pro Leu Met Ser

1 5 10 15

Glu Phe Arg Lys

20

<210> 685

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 685

Arg Asn Ser Tyr Arg Met Asp Gln Thr Phe Lys Ile Leu Tyr Leu Val

1 5 10 15

Ile Leu Gln Thr Gly Ala Pro Gly Met Asn Ile Lys Glu Thr

154

20 25 30

<210> 686

<211> 43

<212> PROTEIN

<213> Homo sapiens

<400> 686

Thr Leu Ser His Gln Ile Lys Ser Ser Phe Gly Gln Lys Met Gly Lys

1 5 10 15

Val Ile Phe Thr Asn Tyr Leu Pro Val Ala Phe Gln Leu Val Ile His

20 25 30

Ser Ala Val Met Trp Gly Arg Gln Leu Lys Ile

35 40

<210> 687

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 687

Leu His Tyr Leu Met Arg Trp Pro Ile Asn Leu Ile Arg Arg Trp Thr

1 5 10 15

Ser Phe Trp Glu Ile

20

<210> 688

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 688

Thr Gly Thr Lys His Glu Ser Gly Leu Leu Lys Thr Ser Gln Lys Leu

1 5 10 15

Ser Leu Lys Lys Met Thr Gln Gly Pro Cys Leu Gly

20 25

<210> 689

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 689

Arg His Pro Pro Gly Ser Thr Arg Tyr Pro Ser Phe Pro Leu Arg Leu

1 5 10 15

Val Pro Leu Ser Leu Ile Gly Pro

20

<210> 690

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 690

155

Lys Arg Ser Ser Arg Leu Gly Trp Ala Ser Gly Arg Ile Thr Tyr Ser

1 5 10 15

His Ser Pro Pro Arg Gly Trp Cys Ser Ala Ala Trp Pro Pro Arg His

20 25 30

Gly Trp Cys

35

<210> 691

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 691

Thr Ser Arg Pro Cys Thr Ser Thr Ser Thr Thr Ser Leu Leu Pro Met

1 5 10 15

Ala Leu Leu Cys Val Lys Arg Gly Arg Arg Cys Thr Lys Ala Lys Ala

20 25 30

Thr Ala Val Ser Ala Ala Val Ser Thr Glu Gln Ile

35 40

<210> 692

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 692

Trp Phe Ala Phe Leu Ser Ile Lys Lys Arg Ala Ala Arg

1 5 10

<210> 693

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 693

Leu Arg Met Ser Leu Ile Leu Ser Val Ile Cys Ser Cys Ser Trp Glu

1 5 10 15

Leu Trp Gly Tyr Met Ile Cys His Ile

20 25

<210> 694

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 694

Thr Pro Ser Ser Asp Pro Glu Ser Pro Gly Met Leu Ala Arg Ser Thr

1 5 10 15

Pro Ser Thr Thr Gly Arg Trp Cys Ala Leu

20 25

156

<210> 695

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 695

Tyr Ile Thr Leu Leu Tyr Leu Arg Lys Ile Phe Lys Val

1 5 10

<210> 696

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 696

Gln Asp Thr Leu Arg Asn Leu Leu Leu Gln Thr Thr Leu Thr Cys Trp

1 5 10 15

Trp Ile His

<210> 697

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 697

Asn Thr His Pro Leu Leu Gln Ala Cys Ser Pro Ser Ala Cys Arg Cys

1 5 10 15

Pro Cys His Gln

20

<210> 698

<211> 14

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 698

Asn Leu Gln Ile Arg Arg Leu Pro Arg Gln Arg Leu Leu Ser

1 5 10

<210> 699

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 699

Cys Ile His Ile Thr Gly Met Ser Leu Leu Gly Leu His Gly Thr Met

1 5 10 15

Glu Lys Ser Glu Gly Met Lys Ser Phe His

20 25

<210> 700

<211> 20

<212> PROTEIN

157

<213> Homo sapiens

<400> 700

Gly Asn Thr Ala Asn Gly Pro Gly Ile Gly Arg Lys Ser Leu Met Pro

1 5 10 15

Met Gln Val Thr

20

<210> 701

<211> 36

<212> PROTEIN

<213> Homo sapiens

<400> 701

Glu Ala Phe Thr Leu Thr Met Val Gly Phe Ser Lys Leu Ile Ser His

1 5 10 15

Val Leu Leu Ser Tyr Lys Pro Phe Ser Lys Gly Lys Glu His Glu Arg

20 25 30

Ile Pro Glu Ser

35

<210> 702

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 702

Gly Ser Trp Gly Val Pro Trp Met Thr Ser Met

1 5 10

<210> 703

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 703

Thr Ser Gly Thr Lys Ser Ser Arg Lys Ser Met Asn Ser

1 5 10

<210> 704

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 704

Ser Ser Lys Lys Arg Lys Lys Gly Asn Met Trp Thr Thr

1 5 10

<210> 705

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 705

Asp Val Ala Gly Glu Glu Asn Glu Gly Pro Pro Arg Pro Ser Leu Met

1 5 10 15

158

Cys Pro Arg

<210> 706

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 706

Pro Glu Ile Phe Leu Arg Met Asn Trp Cys Val Ser

1 5 10

<210> 707

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 707

His Leu His Arg Met Leu Tyr Met Asn Arg Leu Gln Pro Ser Thr Gln

1 5 10 15

Trp Tyr Lys Ile Thr Ala

20

<210> 708

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 708

Gly Leu Pro Gln Lys Glu Leu Lys Gly Ile Gln Leu

1 5 10

<210> 709

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 709

Lys Lys Lys Lys Ser Asn Ile Pro Lys Thr Pro

1 5 10

<210> 710

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 710

Val Ser Pro Ala Phe Leu Asn Phe Met Asp Leu Leu Leu Thr Ile Lys

1 5 10 15

Leu Glu Leu Cys Val

20

<210> 711

<211> 9

<212> PROTEIN

<213> Homo sapiens

159

<400> 711

Asn Ile Ile Phe Leu Ser Asp Pro Gly

15

<210> 712

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 712

Ser Pro Ser Arg Ala His Leu Trp Pro Thr Arg Arg Ser Ser

1 5 10

<210> 713

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 713

Asn Met Val Lys Met Leu Val Phe Ile Leu Lys Leu

1 5 10

<210> 714

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 714

Ile Ala Met Thr Met Asp Gln Tyr Pro Phe

1 5 10

<210> 715

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 715

Pro Pro Phe Phe Thr Ile Asp Leu Val Thr Thr Cys Ile

1 5 10

<210> 716

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 716

Cys Lys Lys Lys Asn Asp Gly Thr

15

<210> 717

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 717

Met Thr Gly Phe Leu Thr Pro Arg Asp Phe

1 5 10

160

<210> 718

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 718

Leu Pro Pro Pro Trp Thr Ala Gln Leu Pro Cys Ile Ser

1 5 10

<210> 719

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 719

Arg Leu Ile Glu Ile Lys Val Pro Ser Gln Lys

1 5 10

<210> 720

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 720

Gly Glu Lys Lys Tyr Asn Val Phe His Met Lys

1 5 10

<210> 721

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 721

Lys Ala Phe Phe Leu Pro Val Arg

15

<210> 722

<211> 109

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 722

Ala His Pro Arg Pro Pro Ala Leu Ser Leu Arg Ser Leu Gln Arg Ser

1 5 10 15

Pro Ala Leu Arg Ala Arg Trp Ser Thr Ser Phe Cys Leu Ala Pro Lys

20 25 30

Arg Pro Leu Leu Leu Thr Pro Arg Glu Gln Thr Trp Leu Asp Pro Asp

35 40 45

Pro Ala Asn Ala Pro Cys Arg Cys Ser Ala Pro Ala Asn Gln Pro Arg

50 55 60

Thr Glu Ser Pro Pro Pro Leu Pro Ala Asp Leu Pro His Ser Ser Gly

65 70 75 80

161

Leu Ala Leu Cys Gly Ile Val Ser Thr Ser Ser His Leu Ile Leu Leu

85 90 95

Trp Leu Leu Gly Ser Arg Met Ala His Pro Arg Leu Pro

100 105

<210> 723

<211> 131

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 723

Ser Thr Ala Thr Ala Pro Trp Thr Ser Ala Ser Ser Thr Arg Ser Ala

1 5 10 15

Lys Trp Ala Thr Ser Thr Gly Ala Arg Cys Leu Thr Ser Gly Ala Ala

20 25 30

Ala Ala Cys Trp Arg Arg Cys Ser Ala Thr Ser Arg Gly Arg Ala Arg

35 40 45

Ser Pro Arg Val Arg Ser Arg Thr Leu Pro Lys Leu Cys Leu Trp Trp

50 55 60

Met Thr Asn Leu Thr Thr Arg Arg Ser Thr Arg Arg Ser Cys Trp Thr

65 70 75 80

Ser Pro Gly Met His Thr Gln Thr Ser Arg Ala Pro Gln Pro Ser Arg

85 90 95

Ala Gln Thr Trp Arg Thr Ser Pro His Cys Gly Ile Asp Thr Pro Val

100 105 110

Trp Leu Ser Gln Asn Cys Leu Arg Ala Pro Leu Thr Arg Thr Gly Arg

115 120 125

Gly Leu Cys

130

<210> 724

<211> 70

<212> PROTEIN

<213> Homo sapiens

<400> 724

Val Lys Thr Gly Ser Ser Trp Thr Gly Ser Cys Arg Gln Gly Trp Arg

1 5 10 15

Trp Ser Gly Ser Asp Cys Gly Ile Ser Ile Thr Thr Thr Thr Pro Arg

20 25 30

Gly Ala Cys Gly Ala Ser Ala Ala Gly Arg Ala Leu Ala Arg Gly Arg

35 40 45

162

Leu Glu Asp Gly Gln Pro Leu Pro Ala Pro Val Gly Val Pro Arg Gln

50 55 60

Leu Leu Gly Ala Gly Leu

65 70

<210> 725

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 725

Ser Ala Thr Arg Met Pro Gly Pro Val Pro Ala Pro Ser

1 5 10

<210> 726

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 726

Gly Pro Pro Thr Ala Gln Cys Gln His Pro Pro Glu Pro Leu Phe Leu

1 5 10 15

Gln Ala Asp Gln Ala Thr Cys Leu Arg Gly Phe Ala Arg Ile Ala Ser

20 25 30

Ser Cys His Pro His His Gln Phe

35 40

<210> 727

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 727

Gly Pro Pro Thr Val Thr Arg Pro Thr Pro Thr Thr Glu Pro Ser

1 5 10 15

Pro Pro Thr Ser Thr Thr Leu Trp Ser Ser Ala Phe Ser Ala Thr Arg

20 25 30

Met Pro Gly Ile Pro Ala Gly Leu Gly Leu Gly Phe Gly Arg Ala Glu

35 40 45

Gly Ala Gln Glu Gly Lys Ala Arg Val

50 55

<210> 728

<211> 81

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 728

Thr Gly Gly Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Thr Lys Met

163

1 5 10 15

Met Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met

20 25 30

Thr Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr

35 40 45

Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser

50 55 60

Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp

65 70 75 80

gly

<210> 729

<211> 68

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 729

Val Pro Gly Leu Arg Ala Lys Val Trp Arg Gly Ser Pro Glu Trp Asp

1 5 10 15

Pro Glu Arg Arg Cys Leu Leu Pro Leu Pro Ser Cys Ala Gly Pro Gly

20 25 30

Asp Gln Ala Arg Met Arg Thr Ser Ala Val Ser Cys Cys Arg Ser Pro

35 40 45

Trp Thr Pro Cys Glu His Phe Pro Arg Pro Arg Ser Cys Gly Trp Arg

50 55 60

Trp Trp ArgGlu

65

<210> 730

<211> 16

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 730

Thr Tyr Gly Ala Trp Ala Ser Arg Trp Trp Ser Trp Gln Gln Asp Ser

1 5 10 15

<210> 731

<211> 117

<212> PROTEIN

<213> Artificial sequence

<220>

164

<223> Modified FSP Homo sapiens

<400> 731

Gln Thr Pro Pro Ser Arg Thr Met Thr Ser Gly Pro Thr Tyr Gly Ala

1 5 10 15

Trp Ala Ser Arg Cys Pro Ala Arg Leu Pro Pro Arg Trp Ser Trp Gln

20 25 30

Gln Asp Ser Phe Pro Thr Arg Thr Ala Arg Arg Thr Leu Arg Ser Ser

35 40 45

Pro Lys Ser Tyr Arg Lys Ser Pro Arg Phe Cys Pro Asp Thr Trp Ala

50 55 60

Ser Arg Gly Thr Ser Ser Pro Ser Ser Lys Thr Ala Leu Leu Lys Ile

65 70 75 80

Thr Gly Arg Asp Gln Ser Ile Ile Ser Tyr Leu Asn Thr Trp Arg Trp

85 90 95

Thr Trp Arg Pro Gly Ser Arg Met Ser Trp Arg Arg Leu Ser His Arg

100 105 110

Gly Leu Ala Ala Ser

115

<210> 732

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 732

Trp Val Leu Ile Cys Leu Asp Leu Phe Arg Gln Lys Phe Arg Lys Pro

1 5 10 15

Leu Met Leu Gln Glu Ser Leu Phe Gln Met Ile Phe Ser Ser Ile Phe

20 25 30

<210> 733

<211> 95

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 733

Leu Pro Met Pro Pro Pro Pro Arg Arg Pro Leu Pro Pro Pro Pro Arg

1 5 10 15

Ala Ala Gln Arg Thr Val Thr Arg Gly Lys Arg Leu Asn Asn Arg Ala

20 25 30

Pro Leu Gly Glu Ala Thr Gly Ser Arg Arg Gln Gly Glu Gln Asn Thr

35 40 45

165

Glu Asp Ser Arg Lys Ala Lys Pro Asp Phe Trp Lys Val Gln Asn Ser

50 55 60

Phe Gly Ser Leu Val Pro Glu Arg Glu Lys Met Leu Val Pro Gly Gly

65 70 75 80

Thr Arg Val Cys Gln His Ala Lys Ile Gly Pro Cys Tyr Met Lys

85 90 95

<210> 734

<211> 64

<212> PROTEIN

<213> Homo sapiens

<400> 734

Asn Arg Phe Arg Leu Cys Ile Thr Ile Leu Pro Gly Met Lys Met Ser

1 5 10 15

Trp Ser Ser Glu Lys Val Met Ser Leu Met Ser Trp Lys Ser Val Met

20 25 30

Thr Ala Gly Leu Trp Gly Pro Gln Glu Glu Pro Asn Ser Leu Val Leu

35 40 45

Ser Pro Glu Thr Thr Ser Arg Gly Cys Glu Leu Arg Ser Leu Leu Leu

50 55 60

<210> 735

<211> 66

<212> PROTEIN

<213> Homo sapiens

<400> 735

Leu Leu Leu Arg Ile Leu Arg Leu Arg Arg His Leu Pro Pro Ala Ala

1 5 10 15

Gly Leu Pro Val Thr Gly Ser Pro Arg Ser Phe Thr Asp Thr Ile Thr

20 25 30

Tyr Arg Asp Ser Arg Phe Leu Arg Pro Ser Lys Arg Pro Ser Trp Tyr

35 40 45

Asn Ile Leu Thr Val Asn Arg Pro Val Val Ile Leu Ala Arg Thr Gln

50 55 60

Thr Thr

65

<210> 736

<211> 81

<212> PROTEIN

<213> Homo sapiens

<400> 736

Leu Pro Leu Pro Pro Val Pro Phe Leu Asp Cys Leu Ser Cys Pro Gly

1 5 10 15

166

Arg Asn Ala Trp Trp Leu Gln Val Pro Gly Glu Leu Gly Thr Leu Ala

20 25 30

Asp Arg Glu Arg Gly Pro Trp Ser Pro Thr His Ser Ala Gln Ser Pro

35 40 45

Val Pro Leu Arg Ile His Phe Cys Pro Val His Arg Pro Pro Trp His

50 55 60

Gly Leu Met Ala Ala Asp Arg Ala Trp Gly Leu Phe Thr Pro Ala Pro

65 70 75 80

Thr

<210> 737

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 737

Asp Gln Trp Cys Pro Asp Gly Ser His Leu Gly Gln Pro Arg Gln Leu

1 5 10 15

Pro Arg Asp Arg Arg Pro Arg Thr Ser His Ile Ser Ala Leu Ile Ala

20 25 30

Ala Arg Gly Leu Cys Ser Ala Ala Ala Ser

35 40

<210> 738

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 738

Asn Leu Ala Ile Val Glu Glu Glu Met Phe Leu Val Met Gly Glu Leu

1 5 10 15

Asn Tyr Val Pro Leu Ala Leu

20

<210> 739

<211> 52

<212> PROTEIN

<213> Homo sapiens

<400> 739

Pro Tyr Phe Trp Arg Thr Leu Gln Pro Met Glu Met His Phe Phe Phe

1 5 10 15

Cys Gln His Phe Pro Ser Gly Pro Thr Arg Val Pro Leu Ser Lys Tyr

20 25 30

Thr Thr Arg Ser Lys Ser Leu Lys Gln Asp Lys Arg Phe Tyr Phe Ser

35 40 45

167

Ile Pro Ser Thr

50

<210> 740

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 740

Arg Arg Cys Glu Val Gln His Ser Ser Val Thr Arg Glu Lys Thr Gly

1 5 10 15

Cys Gly Phe Ser Ala Val Asn Lys Thr Gln Glu Val Phe Trp Trp Lys

20 25 30

Asn Thr Leu Tyr Leu Ile Lys Ile His Tyr Met Lys

35 40

<210> 741

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 741

Pro Gln Pro Gly Asn Gln Arg Ser Asn Arg Ser Leu Pro Gly Thr Thr

1 5 10 15

Arg Met Arg His Arg Val

20

<210> 742

<211> 52

<212> PROTEIN

<213> Homo sapiens

<400> 742

Thr Arg Thr Ser Leu His Gln Ala Thr Arg Lys His Ile Ile Ile Pro

1 5 10 15

Leu Glu Arg Arg Ser Pro Gln Ala His Lys Leu Trp Met Ile Val Ile

20 25 30

Ile Lys Asp Ile Phe Leu Met Lys Arg Phe Leu Thr Leu Ala Ser Ala

35 40 45

His Val Gly Val

50

<210> 743

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 743

Gly Ser Leu Leu Gly Leu Ser Leu Ala Gln Pro Glu Ala Leu Lys Val

1 5 10 15

Ala Ala Arg Leu Arg Cys Gln His Pro Gln His His His Arg Arg Arg

168

20 25 30

Val Gln Cys Ser Leu Ser Arg Val Arg Arg

35 40

<210> 744

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 744

Gly Ser Asn Ser Asn Leu Gly Met Lys Cys Gly Ile Gln Asn Gln Met

1 5 10 15

Leu Val Ile Gln Gln Glu Ala Gly Phe Gly Ile Leu His Thr

20 25 30

<210> 745

<211> 40

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 745

Gln Ser Gln Gly Gly Gly Ala Gly Gly Glu Ser Gln Arg Ala Asn Ser

1 5 10 15

Ala Arg Arg Pro Leu Gly Arg Ile Gln Gly Arg Cys Arg Ala Ala Gly

20 25 30

Pro Ala Cys Gly Arg Gly Arg Ala

35 40

<210> 746

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 746

Pro Glu Gln Leu Thr Leu Pro Ile Ser Gln Gln Lys Pro Ser Pro Val

1 5 10 15

Leu Gly Lys Leu Glu Met Val Lys Ala Val Gln Leu Arg Val Ala

20 25 30

<210> 747

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 747

Phe Met Arg Asn Phe Leu Asn Met Leu Cys Phe Glu Asn Ile Leu Leu

1 5 10 15

His Leu His Gln Ile Met Lys Phe Leu Leu Arg Gln Pro Gly Gln Gly

20 25 30

169

Ile Trp Lys Leu Arg Leu Ile Gln Pro Ser Leu Trp Leu Ser Leu Trp

35 40 45

Ile Arg Pro Asn Leu Leu Leu Phe His Ile

50 55

<210> 748

<211> 32

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 748

His Pro Gln Ser His Leu His Pro Leu Gly Leu Pro His Leu Pro His

1 5 10 15

Pro His Cys Gln Thr Thr Arg Ala Pro Cys Gln Asp Trp Pro Leu Pro

20 25 30

<210> 749

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 749

Pro Pro Ala Gly Lys Asn Leu Met Lys Ser Pro Gln Trp Trp Gly Ser

1 5 10 15

Ser Val His Leu Ala Trp Ser Ser Gln Ser Leu Ser Leu His Gly Ser

20 25 30

Ala Val Arg Glu Asn His Pro Ser Leu Thr Arg Leu Leu His Thr Ser

35 40 45

Leu Cys Met Cys Leu Arg Glu Leu Ile Phe Thr Leu Lys Thr

50 55 60

<210> 750

<211> 53

<212> PROTEIN

<213> Homo sapiens

<400> 750

Met Glu Tyr Cys Pro Pro Leu Asn Ser Cys Ala Ser Val Cys Ala Ser

1 5 10 15

Trp Ser Leu Asp Gly Cys His Pro Leu Trp Ser Trp His Ser Ser Arg

20 25 30

Ala Gly Arg Ala Gly Gly Gln Gly Ala Gln Asp Cys Pro Cys Ile Ala

35 40 45

Glu Leu Trp Gln Cys

50

170

<210> 751

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 751

Leu Trp Leu Trp Ala Gly Trp Thr Val Trp Trp Ser Cys Gly Pro Gly

1 5 10 15

Glu Lys Gly His Gly Trp Leu Pro Ser Leu Pro Thr Met Ala Leu Leu

20 25 30

Leu Leu Arg Phe Ser Cys Met Arg Val Ala Ser Tyr

35 40

<210> 752

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 752

Val Met Pro Leu His His Gly Leu Thr Gln Lys Met Ile Ser Glu Thr

1 5 10 15

Cys Ser Val Arg Ser Phe Met Asp Leu Lys Ile Lys Leu Ala Met Lys

20 25 30

Ile Gly Gly Val Ser Leu Thr Ser Phe Leu Phe Pro

35 40

<210> 753

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 753

Ala Ala Ala Thr Thr Leu Thr Ser Ala Ala Ser Ser Ala Gly Ser Ser

1 5 10 15

Trp Thr Pro Gly Arg Arg Gly Gly Thr Cys Ala Ser Ser

20 25

<210> 754

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 754

Asn Leu Leu Val Ser Thr Gln Leu Gly Thr Ala Ala Ser Pro Thr Gln

1 5 10 15

His Gln Gln Leu Pro Arg

20

<210> 755

<211> 27

<212> PROTEIN

<213> Homo sapiens

171

<400> 755

Trp Arg Glu Ala Trp Arg Arg Ser Ser Trp Pro Arg Trp Thr Met Ala

1 5 10 15

Trp Ala Gln His Gly Ser Pro Ala Ile Gly Gly

20 25

<210> 756

<211> 54

<212> PROTEIN

<213> Homo sapiens

<400> 756

Ser Val Ser Arg Lys Ala Gly Leu Val Ser Ser Thr Lys Pro Leu Val

1 5 10 15

Gln Leu Gly Phe Thr Leu Lys Arg Tyr Arg Ser Phe Leu Asn Ile Glu

20 25 30

Phe Leu Gln Thr Pro Lys Leu Thr Arg Lys Leu Ser Glu Trp Ser Arg

35 40 45

Arg Lys Leu Leu Thr Cys

50

<210> 757

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 757

Val Val Lys Thr Val Ala Leu Gly His Leu Thr Ile Arg Asn Val Ala

1 5 10 15

Ser Val Asp Pro Gly Gln Thr Val Leu Trp Met Glu Ser Leu Cys Leu

20 25 30

<210> 758

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 758

Cys His Trp Gly Ala Ala Pro Gly Ser

15

<210> 759

<211> 25

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 759

Gly Pro Val Tyr Tyr Val Cys Asn His Met Asn Val Ser Ser Val Leu

1 5 10 15

172

Lys Pro Ser Leu Phe Thr Val Pro Ile

20 25

<210> 760

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 760

Thr Ser Gln Arg Ile Pro Cys Ser Leu Gln Lys Lys Met His Leu Lys

1 5 10 15

Tyr Gly Leu Phe Ser Thr Phe Tyr Leu Arg Thr Ser Ile His

20 25 30

<210> 761

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 761

Ala Leu Gln Val Phe Ser Ser Thr Gly Leu Lys Arg Thr Leu Ile Glu

1 5 10 15

Arg Val Ser Met Asp Thr Ala Phe Arg Glu Ser

20 25

<210> 762

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 762

Pro Ala Pro Ala Thr Gln Ala Pro Cys Met Met Thr

1 5 10

<210> 763

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 763

Lys Thr Ala Lys Thr Leu Cys Gly His Arg Met Asp Pro Gln Pro Arg

1 5 10 15

Ser Cys Ser Ser Leu Trp Ser Trp Gln Leu Lys Pro Cys Pro Cys

20 25 30

<210> 764

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 764

Leu Val Lys Val Gln Thr His Ile Ser Val Ile Pro Ser Leu Glu Ser

1 5 10 15

Ser Pro Leu Gly Val Gln Asp Trp Gly Asn Pro Ser Ala Leu Ile Gln

20 25 30

173

Asn Ser

<210> 765

<211> 32

<212> PROTEIN

<213> Homo sapiens

<400> 765

Leu Leu Trp Glu Asp Arg Ala Lys Asn Thr Gln Ala Leu Pro Leu Pro

1 5 10 15

Leu Ser Gly Glu Ala Leu Ser Ile Gly Pro Ala Leu Gln Leu Lys Val

20 25 30

<210> 766

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 766

Arg Lys Ser Leu Ile Leu Cys Lys Thr Asn Leu Leu Val Phe Ile Asn

1 5 10 15

Asp Ser Arg Arg His Leu Asp Ser Met Glu Lys Phe

20 25

<210> 767

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 767

Trp Thr Arg Ser Gln Lys Gly Lys Ala Ser Leu Thr Ile Gly Gly Trp

1 5 10 15

Arg Ile Cys Cys Ser Leu

20

<210> 768

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 768

Arg His Pro Leu Gln Ser Leu His His Pro Pro Cys Ser Leu Ser Ala

1 5 10 15

Ala Thr Gln Ala Ile Pro Pro Arg

20

<210> 769

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 769

174

Val Arg Ile Leu Glu Ile Ser Ser His Phe Leu His Ile Leu Arg Ser

1 5 10 15

Gln Lys Met Ile Gln Leu Asn Leu Thr Val Met Lys Lys Ile Leu Leu

20 25 30

Leu Phe Phe His His His Leu Val

35 40

<210> 770

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 770

Asn Leu Arg Asn Gln Ser Val Ala Met Ala Asp Trp Arg Glu Met Lys

1 5 10 15

Ser Val Ile Val Val Leu Arg Leu Asn Val Asp Leu Gln Ala Val Val

20 25 30

Ile Phe Glu Leu Val Tyr

35

<210> 771

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 771

Arg Ile Lys Ala Ser Ser Met Pro Gln Thr Pro Glu Arg Gln Thr Leu

1 5 10 15

Pro His Leu Thr Ser Leu Pro Ser Arg

20 25

<210> 772

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 772

Leu Arg Thr Lys Leu Cys Phe Ala Pro Cys Thr Asn Gln Arg Glu Phe

1 5 10 15

Met Ser Lys Asn

20

<210> 773

<211> 44

<212> PROTEIN

<213> Homo sapiens

<400> 773

Arg Lys Ser Pro Val Thr Gly Asn Met Arg His Trp Pro Cys Leu Lys

1 5 10 15

Asp Arg Lys Gln Ala Glu Arg Pro Gly Leu Ile Cys Arg Asn Pro Ser

175

20 25 30

Leu Leu Trp Ala Trp Met Met Lys Leu His Asn Tyr

35 40

<210> 774

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 774

Val Ile Pro Pro Ile Gln Cys Phe Ile Leu Met Tyr Phe

1 5 10

<210> 775

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 775

Lys Glu Lys Lys Ser Arg Thr Leu Met Lys Met Ile Ser

1 5 10

<210> 776

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 776

His Pro Leu Pro Lys Met Asn Thr Thr Arg Met Gly Ile Trp Ile Ser

1 5 10 15

Pro Gly Ala Gly Pro Gly Leu His

20

<210> 777

<211> 12

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 777

Asn Lys Trp Asn Ala Met Val Val Leu Asn Lys Gly

1 5 10

<210> 778

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 778

Gln Arg Pro Leu Pro Gly Asn Gly Val Arg Lys Ala Gln Asn Gly Trp

1 5 10 15

Cys Arg His

176

<210> 779

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 779

Leu Trp Ser Ser Arg His Thr Ser Leu Arg Ile Thr His Lys Arg His

1 5 10 15

Gly Ser Ile Pro Leu

20

<210> 780

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 780

Ser Gln Glu Ile Leu Val Ile Ala Leu Leu Leu Lys Arg

1 5 10

<210> 781

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 781

Thr Trp Arg Lys Leu Ala Asp Ser Leu Val Cys Ser Trp Ile Thr

1 5 10 15

<210> 782

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 782

Cys His Leu Val Ser Thr Cys Gln Thr Ile Pro Ser

1 5 10

<210> 783

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 783

Leu Glu Ile Leu Arg Arg Ser Ser Leu Gly Arg

1 5 10

<210> 784

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 784

Leu Glu Asn Leu Asp Ser Arg Gln Lys Phe Pro Phe Asp Leu Ile Ile

1 5 10 15

Met Ala Asn Met Tyr Gln Trp Ile Arg Val Arg

20 25

177

<210> 785

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 785

Leu Pro Ile Met Pro Cys Trp Ser Pro Cys Leu Ala Gly Ser Leu Trp

1 5 10 15

<210> 786

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 786

Met Ser Leu Leu Gln Arg Thr Val Ser Gln Gln

1 5 10

<210> 787

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 787

Lys Gln Lys Lys Asn Trp Gln Ser

15

<210> 788

<211> 19

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 788

Met Leu Lys Asn Val Arg Gly Asn Asn Ser Lys Leu Lys Tyr Arg Arg

1 5 10 15

Lys Arg Lys

<210> 789

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 789

Ser Ala Ile Lys Gln Arg Lys Gln Arg Thr Met Trp Ser Gly Lys Pro

1 5 10 15

Ser Leu Trp Ser Ser Gly

20

<210> 790

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 790

178

Lys Glu Lys Lys Leu Arg Leu Ala Val Leu Val Asn Asn

1 5 10

<210> 791

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 791

Gly Leu Ala Ser Pro Leu Ala Ala Cys Arg Leu Thr Ser Pro

1 5 10

<210> 792

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 792

Ile Ser Lys Lys Gln Asn Ala Leu Ala Glu His

1 5 10

<210> 793

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 793

Gly Gln Lys Lys Ile Arg Lys Thr Ser Gln Val Leu Thr

1 5 10

<210> 794

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 794

Ser Glu Lys Cys Arg Ser Ser Leu Asp Trp Arg

1 5 10

<210> 795

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 795

Glu Lys Met Cys Trp Phe Arg Thr Gly Met

1 5 10

<210> 796

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 796

Ala His Lys Lys Ala Met Lys Lys Ala Thr Lys Ser Ser

1 5 10

<210> 797

<211> 10

179

<212> PROTEIN

<213> Homo sapiens

<400> 797

Phe Thr Pro Phe Leu Val His Lys Met Phe

1 5 10

<210> 798

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 798

Ala Gly Arg Ala Ala Pro Ala Pro Ser Pro Arg Thr Ala Ala Ala Cys

1 5 10 15

Pro Thr Pro Val

20

<210> 799

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 799

Val Arg Tyr Pro Arg Ala Arg Pro Arg

15

<210> 800

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 800

Gln Leu Leu Lys Met Arg Ser Trp Asn

15

<210> 801

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 801

Val Ile His Phe Lys Lys Ser Lys Ile Ile Trp

1 5 10

<210> 802

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 802

Gly Pro Thr Pro Leu Leu Leu Ser Ser Gly Phe Tyr

1 5 10

<210> 803

<211> 10

<212> PROTEIN

<213> Homo sapiens

180

<400> 803

Asn Gln Leu Thr Arg Asp Trp Lys Ala Ser

1 5 10

<210> 804

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 804

Leu Ile Glu Glu Phe Thr His Leu Cys Ser Leu

1 5 10

<210> 805

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 805

Ala Val Glu Lys Ser Pro His Leu Arg Trp Leu Ile

1 5 10

<210> 806

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 806

Ser Leu Pro Asn Leu Lys His Cys Leu Met Met Ser

1 5 10

<210> 807

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 807

Ala Gly Ser Gly Ser Asp Thr Gln Arg Asp

1 5 10

<210> 808

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 808

Thr Asp Lys Lys Val Trp Leu Met

15

<210> 809

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 809

Ile Val Lys Lys Gly Glu Glu Gly Gly Arg Met

1 5 10

<210> 810

181

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 810

Thr Ser Met Thr Ser Thr Trp Thr Gly Leu Leu Arg Ser Arg Cys

1 5 10 15

<210> 811

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 811

Asp Cys Gln Lys Ser His Ser Ser

15

<210> 812

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 812

Arg Ala Phe Phe Ile Ser His Ser Leu

15

<210> 813

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 813

Leu Asn Gln Lys Asn Ser Val Ile

15

<210> 814

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 814

Gln Leu Gln Lys Thr Ala Pro Gly Leu Asn Ile

1 5 10

<210> 815

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 815

Lys Gly Lys Lys Asn Val Leu Arg Leu Thr Ser Cys

1 5 10

<210> 816

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 816

Leu Lys Lys Ile Leu Gln Asp Tyr Gln Ser

182

1 5 10

<210> 817

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 817

Phe Phe Thr Thr Cys Gly Asn Arg Arg Ser

1 5 10

<210> 818

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 818

Leu Ala Gln Trp Ala Pro Ala Ala Ser Pro Ala

1 5 10

<210> 819

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 819

Leu Ala Gly Arg Ala Met Gly Arg Ala Pro

1 5 10

<210> 820

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 820

Thr Gly Lys Lys Asn Cys Ser Ile

15

<210> 821

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 821

Lys Asp Leu Phe Cys Arg Gly Ile Ser Leu

1 5 10

<210> 822

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 822

Gly Thr Pro Pro Ser Pro Val Ser

15

<210> 823

<211> 36

<212> PROTEIN

<213> Homo sapiens

183

<400> 823

Trp Gln Cys Leu Glu Pro Ser Arg Phe Tyr Val Pro Trp Glu Ala Ser

1 5 10 15

Val Val Thr Phe Phe Ala Ala Gly Ser Ala Lys Met Ser Cys Gln Ser

20 25 30

Trp Leu Ala Pro

35

<210> 824

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 824

Ser Thr Ser Ala Ser Cys Ser Ser Ser Phe Ser Leu Val Met Thr Leu

1 5 10 15

Thr Leu Asp Thr Trp Arg Leu

20

<210> 825

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 825

Arg Pro Glu Asn Ser Gln Ile Asn Ser Ser Leu Cys Asn Glu Ala

1 5 10 15

<210> 826

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 826

Ile Arg Lys Lys Arg Cys Val Tyr

15

<210> 827

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 827

Thr Thr Leu Ala Phe Gln Lys Ser Leu Pro Trp Pro Lys Leu Leu Pro

1 5 10 15

Lys Thr Arg Ser Met Thr His Leu Thr Glu Lys Pro Arg Lys Lys Ser

20 25 30

Leu Leu Gly Pro Leu Thr Gln Ile His Gln Ile Leu Leu Ser Lys Phe

35 40 45

Ser Phe Phe Gln Leu Leu Cys Thr Arg Asp

50 55

184

<210> 828

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 828

Met Asn Leu Pro Phe Gly Ser Phe Met Ser Gln

1 5 10

<210> 829

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 829

Pro Asp Pro Pro Thr Gly Ile Gly

15

<210> 830

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 830

Leu Cys Lys Lys Ile Met Met Arg Ser Ile Gln

1 5 10

<210> 831

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 831

Thr Pro Arg Thr Gly Gly Pro Arg Cys Trp

1 5 10

<210> 832

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 832

Pro Thr Phe Phe Ile Ser Pro Asn Lys Thr His

1 5 10

<210> 833

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 833

His Lys Trp Leu Lys Phe Cys Leu Leu Arg Val Val Lys Glu Ser Phe

1 5 10 15

His Glu

<210> 834

185

<211> 214

<212> PROTEIN

<213> Homo sapiens

<400> 834

Val Ala Ala Pro Ser Pro Gly Cys Thr Leu Ala Arg Ala Pro Ser Thr

1 5 10 15

Pro Val Lys Thr Val Trp Ala Thr His Arg Ala Arg Val Pro Pro Ala

20 25 30

Cys Leu Leu His Ser Trp Ile Ser Leu Lys Ser Ser Cys Gln Phe Asn

35 40 45

Lys Met Ala Ser Thr His Tyr His Thr Ser Glu Gly Gln Gln Gly Gln

50 55 60

Gly Pro Gly Gln Arg Gln Gly Arg Ala Leu Pro Gln Ser Pro Ala Val

65 70 75 80

Arg Ala Leu Ala Ala Ser Gly Thr Trp Phe Ile Leu Cys Arg Ser Ser

85 90 95

Leu Pro Ser Pro Thr Leu Trp Arg Arg Arg Gly Ser Gly Thr Ile Met

100 105 110

Gly Pro Arg Leu Arg Glu Glu Val Ala Leu Glu Glu Thr Ala Thr Pro

115 120 125

Ala Arg Ala Leu Glu Ala Pro Thr Pro Pro Ile Thr Thr Ile Thr Thr

130 135 140

Thr Ile Thr Thr Thr Thr Ser Pro Gly Thr Ala Arg Gly Ala Arg Ala

145 150 155 160

Arg Thr Ala Arg Gly Met Gly Gly Thr Arg Pro Ser Pro Gln Ala Gly

165 170 175

Gly Val Pro Met Thr Thr Trp Thr Val Ile Ala Ala Ser Trp Arg Val

180 185 190

Gly Gly Pro Leu Gly Ser Leu Val Val Pro Ser Ala Trp Arg Val Gln

195 200 205

Met Gly Pro Thr Gly Thr

210

<210> 835

<211> 140

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 835

Ser Leu Arg Lys Lys Arg Arg Lys Ser Pro Ile Thr Arg Asn Leu Arg

186

1 5 10 15

His Trp Pro Cys Leu Lys Asp His Lys Trp Ala Val Arg Leu Gly Leu

20 25 30

Ile Cys Arg Asn Pro Gly Leu Leu Trp Ala Cys Met Val Lys Leu Gln

35 40 45

Glu Tyr Gln His Leu Leu Ile Lys Thr Ser Leu Arg Lys Lys Arg Lys

50 55 60

Ser Pro Ile Thr Arg Asn Leu Arg Gln Trp Pro Cys Leu Arg Ala Ser

65 70 75 80

Arg Val His Thr Leu Lys Asp His Arg Trp Ala Val Arg Leu Gly Leu

85 90 95

Trp Lys Ala Val Gln Leu Leu Lys Gly Ser Arg Asn Pro Thr Val Gln

100 105 110

Arg Arg Ser Ser Lys Gly His Glu Cys Leu Val Met Ile Phe Gln Cys

115 120 125

Pro Val Arg Thr Leu Arg Ala His Ser Leu Ile Gln

130 135 140

<210> 836

<211> 90

<212> PROTEIN

<213> Homo sapiens

<400> 836

Gln His Cys Pro Pro Val Pro Gly Ser Ala Ser Leu Phe His Arg Arg

1 5 10 15

Arg Thr Gln Gly His Pro Ala Leu His Gln Cys Leu Ser Leu Thr Met

20 25 30

Ser Ser Ala Trp Pro Pro Thr Val Thr Ile Trp Met Cys Arg His Pro

35 40 45

Arg Pro Gln Leu Ser Leu Thr Leu Pro Gln Leu Ser Leu Thr Gln Pro

50 55 60

Gln Pro Pro Val Lys Val Arg Thr Lys Ala Ala Gly Gly Pro Cys Leu

65 70 75 80

Pro Arg Arg Ala Pro Gln Gly Val Asn Pro

85 90

<210> 837

<211> 39

<212> PROTEIN

<213> Homo sapiens

<400> 837

187

Leu Gly Ser Ile Arg Gly Leu Asn Gly Asn Gln Met Arg Met Glu Ser

1 5 10 15

Leu Pro Ser Thr Ala Gly Thr Glu Asn Gly Thr Ser Arg Gln Gln Leu

20 25 30

Leu Arg Lys Thr Trp Ser Phe

35

<210> 838

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 838

Thr Val Leu Ser Leu Val Thr Ile Ser Arg Phe Val Leu Ser

1 5 10

<210> 839

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 839

Asn Ser Asn Ile Gln Leu Met Thr Thr Arg Asn Phe Trp Pro Met Ala

1 5 10 15

Ser Ala Ile

<210> 840

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 840

Trp Glu Lys Lys Arg Gly Thr Lys Ser Met Lys Val Thr

1 5 10

<210> 841

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 841

Ser Thr Phe Phe His Glu Asp Leu Val Arg Ile Pro

1 5 10

<210> 842

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 842

Trp Glu Lys Lys Trp Val Arg Ser

15

<210> 843

<211> 11

188

<212> PROTEIN

<213> Homo sapiens

<400> 843

Asn Val Pro Leu Thr Lys Leu Ser Glu Val Phe

1 5 10

<210> 844

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 844

Leu Trp Asp Pro Ser Arg Arg Arg Thr Thr Thr Val Pro Ile Ser Thr

1 5 10 15

Ser

<210> 845

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 845

Leu Gln Lys Lys Ile Ser Trp Asn Leu Ser Leu

1 5 10

<210> 846

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 846

Lys Glu Lys Lys Gln Trp Lys Lys Asn

15

<210> 847

<211> 154

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 847

Val Pro Leu Pro Arg Ser Leu Leu Arg Gln Pro Val Gln Leu Arg Thr

1 5 10 15

Glu Pro Ala Arg Ser Ser Cys Ile Ser Thr Ala Ser Thr Thr Glu Ser

20 25 30

Thr Pro Thr Ala Ser Ser Ala Met Ala Val Thr Arg Ser Ser Arg Gly

35 40 45

Cys Trp Pro Ala Pro Ser Arg Pro Thr Ala Ser Thr Pro Ala Gly Arg

50 55 60

Gly Ala Phe Pro Pro Lys Cys Cys Ser Gly Met Cys Thr Ser His His

189

65 70 75 80

Ala Pro Trp Pro Ser Gly Pro Arg Thr Ser Thr Ala Val Pro Gly Ser

85 90 95

Ala Cys Gln Gly Ala Val Thr Pro Thr Ser Ala Glu Ile Trp Phe Leu

100 105 110

Pro Ser Pro Asp Ala Leu Ser Met Gly Ser Ser Ser Pro Gln Trp Leu

115 120 125

Gly Thr Leu Leu Trp Thr Phe Cys Gly Leu Arg Trp Gly Cys Pro Trp

130 135 140

Pro Pro Cys Ser Ser Trp Arg Gln Pro Gln

145 150

<210> 848

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 848

Gln Glu Leu Lys Glu Ala Val Lys Phe Ser Ile Val Lys Asn Asn Ser

1 5 10 15

Asn Asn Lys Cys Gly Ile Gln Pro Gly Leu Pro Ile Leu

20 25

<210> 849

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 849

Val Ser Gly Ser Trp Ser Leu Gly His Pro Trp Cys Cys Leu Gly Leu

1 5 10 15

<210> 850

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 850

Leu Thr Leu Asn Ser Leu Arg Trp Pro Leu Gln Leu Ser Asn Leu Ala

1 5 10 15

Gly Ser Gln Ile Ser Trp

20

<210> 851

<211> 29

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 851

190

Ser Glu Arg Cys Leu Leu Val Cys Leu Leu Ser Leu Cys Lys Met Gly

1 5 10 15

Pro Thr Ile Leu Leu Thr Phe Gly Val His Gln Met Gly

20 25

<210> 852

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 852

Arg Pro Cys Trp Ser Arg Cys Ser Leu Arg Gln Met

1 5 10

<210> 853

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 853

Cys Val Gly Cys Gln Phe Ile Val Pro Thr Arg Gly Ala Pro Leu Met

1 5 10 15

Ala Arg Val His Thr Glu His Ser Arg Val Leu Leu Ser Pro Ser Phe

20 25 30

Thr Cys

<210> 854

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 854

Asn Tyr Arg Lys Leu Tyr Gln Lys Gln Leu Glu Lys Arg Ile Lys His

1 5 10 15

Leu Cys Ser

<210> 855

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 855

Ser Ala Thr Pro Leu Pro His Tyr Leu Ala His

1 5 10

<210> 856

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 856

Thr Ser Ser Leu Thr Met Glu Ser Ser Gly His

191

1 5 10

<210> 857

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 857

Leu Met Phe Phe Gly Asn Gln Ser Ile Ile Thr Leu

1 5 10

<210> 858

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 858

Leu Asn His Gln Lys Gly Lys Phe Met Asn Phe

1 5 10

<210> 859

<211> 160

<212> PROTEIN

<213> Homo sapiens

<400> 859

Ala Ile Arg Gly Leu Thr Val Ala Ser Ser Leu Pro Trp Thr Thr Pro

1 5 10 15

Ala Ser Gly Gly Val Ser Arg Tyr Ile Ser Arg Cys Ala Pro Pro Ala

20 25 30

Thr Ala Trp Thr Ser Trp Pro Thr Ala Thr Trp Trp Ala Cys Ala Thr

35 40 45

Arg Arg Met Lys Leu Arg Ser Trp Leu Arg Arg Trp Arg Phe Lys Thr

50 55 60

Ala Pro Met Lys Met Gly Arg Cys Ser Cys Gly Gln Gly Ser Cys Ser

65 70 75 80

Thr Ile Ser Gln Asn His Thr Pro Thr Val Arg Leu Leu Glu Leu Pro

85 90 95

Thr Thr Glu His Cys Pro Leu Thr Ser Ala Thr Ser Cys Glu Leu Gly

100 105 110

Met Val Val Arg Thr Thr Ser Ser Pro Cys Ser Arg Ala Thr Ala Ser

115 120 125

His Pro Pro Gly Cys His Cys Gly Lys Val Ser Thr Ser Thr Pro Thr

130 135 140

Phe Leu Ala Arg Pro Leu Pro Trp Pro Leu Pro Ser Thr Gln Met Ser

145 150 155 160

<210> 860

192

<211> 85

<212> PROTEIN

<213> Homo sapiens

<400> 860

Arg Gly Arg Thr Ser Gly Arg Ser Leu Ser Cys Cys Pro Arg Pro Arg

1 5 10 15

Cys Arg Pro Ala Val Ala Ser Arg Ser Thr Ala Pro Ser Pro Arg Ala

20 25 30

Gly Ser Arg Arg Cys Cys Leu Arg Thr Ser Cys Gly Ala Ala Arg Pro

35 40 45

Arg Arg Thr Arg Ser Ala Trp Gly Asp Trp Val Ala Ser Pro Pro Thr

50 55 60

Arg Ser Ser Ser Arg Thr Ala Cys Gly Ala Ala Ser Pro Pro Ala Arg

65 70 75 80

Ser Trp Ser Ala Pro

85

<210> 861

<211> 60

<212> PROTEIN

<213> Homo sapiens

<400> 861

Leu His Leu Tyr Leu Gln Asp Leu Asp Phe Ile Phe Ile Asn Lys Gly

1 5 10 15

Leu His Leu Lys His Ser Ile Gln Asp Val Gln Phe Met Lys Ile Thr

20 25 30

Met Ala Lys Gln Leu Leu Lys Ile Pro Cys Met Thr Pro Thr Gln Ser

35 40 45

Gln Trp Lys Gly Arg Arg Tyr Asp Leu Ile Pro Thr

50 55 60

<210> 862

<211> 105

<212> PROTEIN

<213> Homo sapiens

<400> 862

His Thr Ala Thr Lys Ala Tyr Thr Ser Pro His Ala Glu Gly Glu Phe

1 5 10 15

Cys Pro Gly Glu Thr Asp Thr Pro Asp Met Gly Glu Gly Leu Ser Gly

20 25 30

Asn Thr Glu Thr Lys Arg Glu Arg Gly Phe Arg Thr Ser Pro Pro Phe

35 40 45

Ile Ser Lys Pro Gln Arg Ala Leu Trp Ser Pro Pro Cys Pro Ser Pro

193

50 55 60

Gly Pro Pro Ser Leu Thr Ser Gly Leu Ser Cys Ser Trp Cys Ala Pro

65 70 75 80

Cys Thr Gly Ala Gly Trp Val Gly Lys Trp Ala Gly Leu Gln Leu Pro

85 90 95

Phe Tyr Pro Gln Trp Gln Leu Pro Pro

100 105

<210> 863

<211> 63

<212> PROTEIN

<213> Homo sapiens

<400> 863

Glu Lys Ala Cys Gly His Pro Thr Asp Arg Ala Glu Thr Leu Leu

1 5 10 15

Lys Phe Leu Leu Lys Gly Ile Leu Ala Leu Ala Ile Gly Pro Ala Gly

20 25 30

Ala Leu Ala Gly Pro Val Leu Thr Thr Leu Gly Thr Gln Arg Pro Leu

35 40 45

Pro Trp Ala Ala Pro Cys Pro Ala Thr Ala Ser Pro Ser Pro Leu

50 55 60

<210> 864

<211> 35

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 864

Ala Ala Ala Arg Trp Lys Ala Cys Leu Trp Pro Val Ala Pro Arg Cys

1 5 10 15

Ser Arg Trp Pro Pro Ala Ile Leu Pro Ala Ser Thr Pro Pro Ser Ala

20 25 30

Pro Pro Thr

35

<210> 865

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 865

Thr Ala Leu Pro Arg Ser Ser Ser Gln Gly Ser Val Trp Gln Leu Pro

1 5 10 15

Gly Asn Gly Leu Gln Ser Gln Ala Gly Ala Leu Arg Met Ile Ser

20 25 30

194

<210> 866

<211> 23

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 866

Gln Gln Val Pro Gly Leu Met Lys Thr Gly Pro Ser Trp Met Glu Ala

1 5 10 15

Trp Asn Gln Met Gly Pro Thr

20

<210> 867

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 867

Ala Ser Arg Trp Asn Pro Leu Arg Arg Thr Arg Ala Leu Arg Gly Pro

1 5 10 15

Pro Arg Gln Arg Ala Ala Ala Pro His

20 25

<210> 868

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 868

Tyr Ser Tyr Pro Ser Ser Leu Ser Val Phe Ser Val Pro Gly Tyr His

1 5 10 15

Leu Tyr Tyr Phe Arg

20

<210> 869

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 869

Ile Phe Tyr Ile Phe Ser Trp Ile Cys Pro Pro Thr Lys Lys Gln Arg

1 5 10 15

His Lys Ile Gln Leu

20

<210> 870

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 870

Ser Ile Gln His Leu Gln Ala Pro Ser Pro Ser Leu Trp Pro Trp Asn

195

1 5 10 15

Met Leu Cys

<210> 871

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 871

Ser Ala Ser Lys Asn Gly Asn Lys Lys Asn Ser Arg

1 5 10

<210> 872

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 872

Glu Ser Leu Lys Asn Thr Leu Asn Gln

15

<210> 873

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 873

Asn Thr Leu Asn Gln Ser Pro Glu His Ala Thr Arg

1 5 10

<210> 874

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 874

Leu Thr Asp Phe Phe Val Leu Trp Lys Ala Ser Gly Thr Ile Gln Phe

1 5 10 15

Asn Cys Lys

<210> 875

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 875

Leu Ala Ala Phe Leu Asn Phe Val Met His

1 5 10

<210> 876

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 876

196

Pro Pro Lys Lys Phe Ser Ile Leu Pro Ser Ser Arg

1 5 10

<210> 877

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 877

Trp Asp Gln Arg Lys Arg Lys Met Gly Lys Val Ile Ser Lys Gly Asn

1 5 10 15

Asp Leu Ser Lys Thr Gly

20

<210> 878

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 878

Trp Ala Ser Thr Ile Arg Asp Arg Cys Val

1 5 10

<210> 879

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 879

Cys Gln Leu Ile Glu Asn Val Pro Leu Leu Leu

1 5 10

<210> 880

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 880

Lys Thr Pro Pro Gly Leu Leu Arg Asn Thr Gln Gln

1 5 10

<210> 881

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 881

Ala Gly Ala Arg Gly Glu Ile Ala Val Met

1 5 10

<210> 882

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 882

Glu Lys Ile Pro Arg Gly Pro Ser Ala Ser

1 5 10

197

<210> 883

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 883

Gln Leu Pro Lys Met Ala Pro Phe Met Met

1 5 10

<210> 884

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 884

Lys Asp Lys Lys Gln Ile Met Lys Cys

15

<210> 885

<211> 165

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 885

Thr Asn Ala Leu Thr Leu Ala Gln Trp Ser Pro Val Ile Ala Ala Ser

1 5 10 15

Pro Ser Ala Ser Thr Gln Ala Arg Cys Ala Thr Ser Ala Ala Ala Thr

20 25 30

Thr Ser Pro Pro Thr Ser Ala Pro Thr Gln Ala Lys Ser Pro Ser Pro

35 40 45

Ala Thr Ser Val Glu Glu Ser Leu Pro Gly Ala Met Asn Ala Arg Gly

50 55 60

Ile Pro Arg Ser Thr Cys Gly Arg Arg Thr Arg Lys Gln Thr Lys Val

65 70 75 80

Leu Trp Pro Leu Arg Pro Pro Pro Leu Ser Leu Pro Thr Arg Pro Arg

85 90 95

Leu Leu Pro Leu Thr Arg Pro Arg Leu Leu Pro Leu Ile His Pro Arg

100 105 110

Pro Pro Pro His Thr His Leu Pro Ala Pro Arg Pro Thr His Pro Leu

115 120 125

Cys Thr Val Ala Ser Pro Pro Arg Arg Trp Pro Pro Arg Thr Pro Leu

130 135 140

Phe Pro Leu Leu Ser Arg Pro Arg Ser Ala Ala Ser Leu Ala Pro Pro

145 150 155 160

198

Gln Gly Phe Arg Thr

165

<210> 886

<211> 49

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 886

Gly Ala Trp Glu Leu Gly Gly Leu Ala Arg Trp Gly Pro Leu Cys Thr

1 5 10 15

His Pro Gln Cys Gln Val Leu Leu Leu Val Glu Asp Val Leu Cys Ala

20 25 30

Lys Trp Phe Ile Cys Pro Ala Ser Phe Asn Pro Gln Asn Asp Ser Met

35 40 45

Arg

<210> 887

<211> 34

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 887

Arg Asn Leu Ile Leu Met Ile Lys Val His Lys Phe Leu His Asn Leu

1 5 10 15

Ser Lys Tyr Thr Asp Lys Leu Gln Lys Ala Arg Met Leu Leu Pro Pro

20 25 30

Lys Ala

<210> 888

<211> 80

<212> PROTEIN

<213> Homo sapiens

<400> 888

Arg Lys Gln Leu Tyr Ile Gln Asn Ala Leu Asn Leu Ser Arg Pro His

1 5 10 15

Ser Ala Arg Leu Thr Ala Ser Asp Thr Thr Arg Thr Arg Thr Cys Arg

20 25 30

Thr Trp Arg Arg Ser Ser Ala Ser Ala Ala Ser Ala Trp Arg Arg Ser

35 40 45

Phe Ala Ala Ser Cys Thr Arg Gly Thr Ser Cys Thr Pro Trp Arg Ala

50 55 60

199

Lys Ile Ser Thr Met Ser Thr Tyr Arg Glu Lys Leu Ser Gly Phe Pro

65 70 75 80

<210> 889

<211> 100

<212> PROTEIN

<213> Homo sapiens

<400> 889

Leu Pro Trp Met Val Pro Trp Gly Met Glu Ala Leu Gly Ser Trp Gly

1 5 10 15

Gln Thr Asp Leu Thr Val Leu Gly Gln Pro Gly Val Arg Ala Leu Gly

20 25 30

Lys Trp Leu Gln Trp Pro His Pro Asp Pro Ala Gly Ser Pro Arg Pro

35 40 45

Gly Pro Arg Gly Gly His Pro Gln Pro Arg Pro Ser Thr Pro Ala Ser

50 55 60

Pro Gly Asn Arg Gly Leu Pro Leu Pro Pro Pro Val Trp Ala Val Leu

65 70 75 80

Ala Trp Ala Arg Ala His Leu Ala Val Glu Ala Cys Leu Arg Thr His

85 90 95

Gly Cys Ser Gly

100

<210> 890

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 890

Ser Arg Pro Arg His Arg Val Arg Lys Arg Arg Cys Pro His Cys Arg

1 5 10 15

Ser Ser Leu Pro Gly Arg His Arg Leu Gln Ser Cys Arg Pro Ser Pro

20 25 30

Asp Pro Arg

35

<210> 891

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 891

Ala Pro Ala Cys His Trp Leu Ala Arg Leu Gly Trp Arg Val Ser Glu

1 5 10 15

Glu Pro Val Leu Ser Ser Leu Thr Ser Leu Arg Ile Glu Leu Leu Leu

20 25 30

200

Ser Ser Cys Ser Ser Arg Trp Gly

35 40

<210> 892

<211> 62

<212> PROTEIN

<213> Homo sapiens

<400> 892

Met Lys Met Ala Cys Gly Lys Arg Ser Leu Leu Gln Glu Val Lys Lys

1 5 10 15

His Glu Leu Pro Lys Lys Ala Pro Ser Trp Pro Asp Arg Pro Leu Val

20 25 30

Gly Leu Ala Arg Ile Gln Ile Trp Ile Leu His Leu Leu Gly Leu Arg

35 40 45

Pro Pro Ser Leu Leu Ser Val Phe Gln Met Thr Phe Ile Lys

50 55 60

<210> 893

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 893

Ala Ala Ala Pro Arg Ser Thr Pro Arg Ala Thr Arg Pro Ser Cys Ala

1 5 10 15

Gly Pro Ser Arg Arg Ala Ala Arg Ala Ser Thr Ala Lys Ser Ala Ser

20 25 30

Ser Arg Met Ala Ser Thr Ser Cys Ala Ala

35 40

<210> 894

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 894

Arg Ala Gly Asn Phe Ala Ile Met Glu Ile Gln Cys Pro Ala Leu Arg

1 5 10 15

Lys Thr Leu Pro Ile Leu Phe Gly Ser Leu Arg Arg Gln Asn Met Ser

20 25 30

Leu Glu Met Trp Cys Arg

35

<210> 895

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 895

201

Arg Val Trp Ser Phe Leu Phe Leu Arg Gln Arg Ala Thr Ser Thr Ile

1 5 10 15

Thr Ile Ala Cys Thr Lys Glu Ser Leu Asn Ser Gln Asn Ser Ser Phe

20 25 30

Ile Phe Ser Leu Leu Ile

35

<210> 896

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 896

Lys Asp Gln Arg Ile Ile Lys Val Ala Pro Leu Val Glu Asp Lys Met

1 5 10 15

ile

<210> 897

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 897

Ile Ala Gln Leu Met Lys Asp Gln Arg Ile Ile Lys Val Pro Gln Ile

1 5 10 15

Val Cys Gly Leu Arg Ile Val Leu Arg Lys Lys Arg Thr Val Phe Leu

20 25 30

Arg Gln

<210> 898

<211> 31

<212> PROTEIN

<213> Homo sapiens

<400> 898

Ile Gln Asp Gln Asp Gln Glu Leu Ala Thr Val Leu Leu Gln Ser Ala

1 5 10 15

Leu Asp Cys Ile Phe Gly Val Glu Glu Met Ala Thr Lys Lys His

20 25 30

<210> 899

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 899

Pro Ser Leu Asn Leu Ile Ser His Gln Ser Ser Val Gly Cys Ser Lys

1 5 10 15

202

Gly Ile Trp Arg Ala Pro Glu Met Thr Leu Phe Ser Thr Pro Thr Phe

20 25 30

Ala Ala Ser Ser Pro Trp Ala Ser Met Ser Trp Val Ser Ser Gly Cys

35 40 45

Ser Leu Pro Leu Trp Ser Ser Ser Cys Cys

50 55

<210> 900

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 900

Cys Thr Leu Gly Gly Ser Val Thr Gln Lys Leu Lys Met Thr Ala Thr

1 5 10 15

Gly Val Pro Val Ile Pro Pro Leu Val Ile Ser Trp Trp Ala Gly Thr

20 25 30

Arg Ser Leu Trp Leu Leu Leu Pro Val Gly

35 40

<210> 901

<211> 40

<212> PROTEIN

<213> Homo sapiens

<400> 901

Gly Gly Thr Gly Thr Asp Met Thr Thr Gln Lys Leu Met Met Val Pro

1 5 10 15

Asn Gln Cys Lys Leu Glu Leu Glu Leu Ser Leu Arg Asn Tyr Ala Leu

20 25 30

Glu Ser Ser Gln Val Pro Met Lys

35 40

<210> 902

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 902

Asn Leu Leu Gln Lys Glu Ile Pro Leu Leu Gln Leu Ser Leu Leu Thr

1 5 10 15

Asp Ser Lys Arg Asn His Gly His Pro Gln Leu Thr

20 25

<210> 903

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 903

Asn Val Leu Cys Lys Gln Gln Asn Gly Leu Pro Ala Pro Glu His Val

203

1 5 10 15

Gly Trp Glu Tyr Leu Thr Gly

20

<210> 904

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 904

Gly Lys Gly Met Lys Lys Ile Lys Lys Cys Leu His Ala Asn Gln Gln

1 5 10 15

Glu

<210> 905

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 905

Asn Asn Leu Cys Gln Arg Leu His Trp Arg Leu Arg His Val Ile Val

1 5 10 15

Leu Ile

<210> 906

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 906

Glu Gln Leu Phe Leu Lys Leu Gln Glu Ala Asn Gly Ile

1 5 10

<210> 907

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 907

Met Leu Thr Ala Leu Pro Pro Ala Met Thr Ala Met Cys Gly Tyr Gly

1 5 10 15

Ile Arg Gly Asn Pro Val Gln Gln Trp Asn Ile

20 25

<210> 908

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 908

Leu Asn Val Thr Cys Phe Val Ile Phe Arg Arg Val Arg Asn Glu Leu

1 5 10 15

204

Gln Lys Trp

<210> 909

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 909

Val Ile Met Ser Ile Thr Gly Lys Lys Glu Glu Ser Asn Arg Ser Lys

1 5 10 15

Ala Leu Ser Glu Ala Thr Pro Phe His Trp Ile Thr

20 25

<210> 910

<211> 27

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 910

Leu Ala Val Ser Ala Val Thr Gly Arg Ala Arg Ser Leu Gly Leu Ser

1 5 10 15

Lys Val Ser Ser Glu Pro Gly Leu Met Ser Thr

20 25

<210> 911

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 911

Leu Lys Thr Phe His Met Lys Cys Phe Leu Asn Leu Ser Gln Met Glu

1 5 10 15

<210> 912

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 912

Pro Asp Lys Lys Ser Arg Asn Cys

15

<210> 913

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 913

Asn Ile Arg Ala Arg Gln Glu Gly Asn

15

<210> 914

<211> 13

205

<212> PROTEIN

<213> Homo sapiens

<400> 914

Leu Ser Asn Ile Arg Ala Arg Gln Glu Gly Lys Ser Gly

1 5 10

<210> 915

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 915

Ala Arg Gly Thr Ser Pro Ile Arg His Cys Ser His Ser Ser Ser Cys

1 5 10 15

Ser Gly Pro Ser Ser

20

<210> 916

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 916

Phe Arg Glu Lys Arg Asn Tyr Val Lys Lys Lys Ser Lys Leu Lys Lys

1 5 10 15

Lys Glu Lys Lys

20

<210> 917

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 917

Ser Arg Thr Ala Leu Ile Thr His Thr Leu Cys Cys Trp Leu Thr Trp

1 5 10 15

Ala Gln Val Ser Ala Phe

20

<210> 918

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 918

Arg Arg Arg Ser Leu Pro Cys Gly Trp Thr Leu Leu Tyr Leu Ser Ser

1 5 10 15

Ala Trp Arg Met Lys

20

<210> 919

<211> 9

<212> PROTEIN

<213> Homo sapiens

206

<400> 919

Leu Arg Pro Pro Trp Pro Ser Trp Ser

15

<210> 920

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 920

Ile Gln Ala Ser Thr Ser Pro Ser Ser Gln His Gly Cys Met Arg Asp

1 5 10 15

Leu Pro

<210> 921

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 921

Glu Val Thr Arg Cys Cys Phe Phe His Lys

1 5 10

<210> 922

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 922

Ala Pro Ser Leu Thr Phe Ser Pro Ser Gly Leu Met Glu Ile Trp Ile

1 5 10 15

Ser Ala Ser Val

20

<210> 923

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 923

Val Ile Leu Gln Val Arg Ser Thr Ala Cys Glu Asn Ser Pro Lys

1 5 10 15

<210> 924

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 924

Ile Ser Lys Lys Cys Ser Leu Ser His Gln Glu Lys

1 5 10

<210> 925

<211> 18

<212> PROTEIN

207

<213> Homo sapiens

<400> 925

Leu Arg Phe Leu Arg Thr Met Lys Gln Trp Pro Lys Phe Cys Ser Ala

1 5 10 15

Gly Ile

<210> 926

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 926

Thr Pro Leu Lys Asn Glu Asp Phe Ile Ser Cys

1 5 10

<210> 927

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 927

Trp Thr Cys Leu Lys Met Ser Ile Gly Ala

1 5 10

<210> 928

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 928

Asp Gly Trp Gly Ala Met Ala Leu Thr Arg Gly

1 5 10

<210> 929

<211> 11

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 929

Ala Trp Lys Ala Val Leu Val Glu Arg Gly Pro

1 5 10

<210> 930

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 930

Asn Ser Leu Gly Ile Ile Trp Pro Lys Ser Gly Leu Ile Leu Thr Ala

1 5 10 15

<210> 931

<211> 14

<212> PROTEIN

208

<213> Homo sapiens

<400> 931

Pro Trp Lys Asn Ser Glu Ser Met Lys Lys Val Lys Cys Ile

1 5 10

<210> 932

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 932

Ile Tyr Ile Phe Cys Leu His Tyr Gly Thr Gln Leu

1 5 10

<210> 933

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 933

Phe Ser Lys Lys Ala Arg Lys Gln Ser

15

<210> 934

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 934

Gln Asn Lys Lys Val Met Ser Met Glu Met Ile Leu

1 5 10

<210> 935

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 935

Ser Gln Lys Thr Val Ser Ser Gly Lys Val

1 5 10

<210> 936

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 936

Tyr Ser Lys Lys Ala Lys Glu Arg Gln Asn

1 5 10

<210> 937

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 937

Ile Asn Lys Lys Thr Ser Arg Ile Phe Pro Ala

1 5 10

209

<210> 938

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 938

Leu Asn Lys Met Asn Ser Gly Trp Ser Leu Lys

1 5 10

<210> 939

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 939

Leu Ala Phe Phe Thr Thr Tyr Met Ala

15

<210> 940

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 940

Arg Ser Gln Gly Ala Gly Pro Val Ala Thr

1 5 10

<210> 941

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 941

Lys Val Lys Lys Gln Asp Pro Lys Pro Ile Asn

1 5 10

<210> 942

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 942

Leu Val Gly Gly Ala Gly His Gly Arg Trp

1 5 10

<210> 943

<211> 193

<212> PROTEIN

<213> Homo sapiens

<400> 943

Ser Val Ser Cys Gly Ser Asn Thr Thr Thr Cys Ile Trp Leu Pro His

1 5 10 15

Pro Arg Arg Thr Arg Ala Cys Arg Trp Ser Phe Leu Ser Ser Ile Arg

20 25 30

Trp Cys Arg Cys Phe Pro Ser Thr Ser Arg Ser Trp Arg Arg Arg Ala

35 40 45

210

Ser Gly Thr Thr Leu Leu Ser Ser Thr Ser Cys Trp Thr Ser Ser Trp

50 55 60

Thr Ser Ala Thr Pro Arg Pro Pro Thr Ala Arg Ser Cys Arg Ser Thr

65 70 75 80

Ser Leu Arg Lys Ala Thr Ser Trp Lys Gln Gly Pro Arg Gly His Gln

85 90 95

Pro Pro Ser Pro Thr Arg Cys Pro Gly Gly Pro Lys Ala Ser Ser Ile

100 105 110

Gly Arg Met Arg Cys Ser Trp Thr Ser Ser Ser Leu Ser Thr Ser Trp

115 120 125

Val Asn Thr Gln Glu Trp Asp Gly Trp Val Thr Arg Ser Ala Pro Thr

130 135 140

Ala Met Ser Cys Ala Ala Arg Ser Trp Ala Pro Ser Arg Cys Glu Ser

145 150 155 160

Ser Ser Arg Ala Cys Pro Ser Cys Ala Trp Ala Ser Thr Thr Arg Ser

165 170 175

Ser Leu Thr Thr Arg Ala Ala Ala Lys Ala Asn Pro Trp Ser Trp Arg

180 185 190

Met

<210> 944

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 944

Ser Ser Ser Leu Ser Thr Ser Trp Ser Ala Pro Thr Ala Met Ser Cys

1 5 10 15

<210> 945

<211> 82

<212> PROTEIN

<213> Homo sapiens

<400> 945

Arg Thr Pro Ala Ser Cys Val Arg Thr Leu Thr Trp Thr Trp Gln Trp

1 5 10 15

Ser Val Pro Ile Arg Glu Cys Ser Ser Thr Lys Ala Ser Val Ala Arg

20 25 30

Gln Pro Pro Gly Cys Ser Trp Arg Ser Arg Ser Thr Leu Ser Leu Ser

35 40 45

Gly Gly Ala Trp Ser Met Pro Arg Asn Gly Pro Trp Glu Thr Pro Ser

211

50 55 60

Met Ser Lys Gln Asn Arg Gly Leu Arg Leu Ile Lys Ser Ser Ser Thr

65 70 75 80

Lys Ser

<210> 946

<211> 174

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 946

Ser Ser Ser Arg Thr Pro Arg Arg Gln Arg Ser Ser Thr Thr Pro Pro

1 5 10 15

Ala Ser Ser Gly Thr Ser Ala Gln Pro Leu Arg Pro Leu Ala Arg Arg

20 25 30

Cys Gly Ser Ala Thr Gly Trp Trp Thr Pro Trp Ser Pro Leu Ser Thr

35 40 45

Thr Pro Trp Thr Arg Ala Asn Ala Arg Thr Arg Ala Trp Arg Thr Arg

50 55 60

Cys Ala Ser Cys Gly Thr Cys Pro Thr Ala Ser Thr Thr Arg Cys Arg

65 70 75 80

Arg Pro Arg Cys Ser Gly Trp Arg Val Ala Ala Ala Gly Thr Trp Arg

85 90 95

Gly Arg Arg Arg Glu Arg Ser Trp Ala Ala Ser Arg Arg Arg Ala Gly

100 105 110

Gly Cys Ala Ser Cys Pro Ser Pro Pro Met Arg Ser Pro Ser Arg Arg

115 120 125

Cys Pro Arg Thr Pro Arg Ala Ser Ser Gly Cys Gly Ala Pro Arg Ser

130 135 140

Trp Gly Cys Thr Thr Gly Cys Cys Ser Ala Ala Arg Pro Pro Gly Arg

145 150 155 160

Cys Arg Thr Ser Arg Gln Ala Thr Ala Gly Gly Arg Gly Cys

165 170

<210> 947

<211> 60

<212> PROTEIN

<213> Homo sapiens

<400> 947

Gly Pro Ser Trp Thr Trp Gly Arg Ala Pro Arg Thr Glu Ser Ser Gln

212

1 5 10 15

Pro Arg Gly Ser Ser Ser Cys Ser Ala Arg Trp Cys Leu Gly Arg Cys

20 25 30

Cys Cys Ser Gly Asn Asp Gly Arg Thr Arg Ser Ser Gly Trp Met Pro

35 40 45

Gly Met Asn Met Lys Met Lys Thr Phe Met Lys Ala

50 55 60

<210> 948

<211> 163

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 948

Ser Leu Leu Val Trp Pro Val Gly Arg Asn Trp Gln Leu Ala Gly Pro

1 5 10 15

Leu Thr Pro Thr Arg Gly Leu Thr Arg Thr Thr His Pro Gly Val Pro

20 25 30

Arg Gly Ser Leu Met Thr Trp Pro Leu Thr Gly His Gln Arg Gly Ala

35 40 45

Trp Pro Pro Gly Leu Pro Pro Glu Pro Glu Val Pro Pro Ala Leu Glu

50 55 60

Cys Trp Asp Pro Thr Pro Gln Ser Pro Ser Trp Pro Leu Gln Phe Leu

65 70 75 80

Gly Pro Leu Ala Pro Ala His His Ser Gly Ser His Ser Glu Tyr Pro

85 90 95

Met Ser Ser Ser Gly Leu Pro Cys Ser Trp Trp Trp Thr Gln Ala Thr

100 105 110

Pro Ala Pro Thr Trp Thr Thr Ser Ser Arg Leu Ala Arg Ala Pro Arg

115 120 125

Ala Ser Cys Ala Ser Pro Pro Cys Ala Ala Arg Ala Ser Trp Trp Pro

130 135 140

Ser Arg Arg Trp Thr Cys Ala Ser Ser Arg Gly Ala Ser Cys Ser Ser

145 150 155 160

Thr Arg Trp

<210> 949

<211> 74

<212> PROTEIN

<213> Homo sapiens

213

<400> 949

Met Asp Thr Leu Ala Leu His Asp Pro His Pro Met Ala Thr Ser Gly

1 5 10 15

Gly Leu Ser Leu His Pro Asp Pro Leu Pro Gly His Gln Phe Pro Leu

20 25 30

Glu Ala His Phe Glu Ala Leu Ser Leu Ser Lys Phe Thr Phe Ser Phe

35 40 45

Leu Leu Leu His Phe Pro Asn Ile Phe Ser Ile Pro Trp Thr Asn Gln

50 55 60

Arg Cys Cys Ser Ser Leu Gly Gln Arg Tyr

65 70

<210> 950

<211> 52

<212> PROTEIN

<213> Homo sapiens

<400> 950

Asn Lys Glu Thr Phe Trp Cys Glu Arg Val Met Gly Asn Leu Val Asn

1 5 10 15

Met Ser Phe Leu Tyr Ile Leu Met Asp Arg Gly Asp Ile Leu Ser Tyr

20 25 30

Asn Met Leu Ile Thr Cys Ile Asp Ser Arg Ala Leu Gly Phe Gln Thr

35 40 45

Phe Leu Asn Leu

50

<210> 951

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 951

Cys His Pro Leu Trp Glu Gln Leu Asp Thr Glu Pro Thr Pro Arg Leu

1 5 10 15

Cys Ser Ala Arg Lys Glu Asn Gln Arg Trp Ser Leu Arg Pro Ala Ala

20 25 30

Arg Arg Pro Trp Arg Ser

35

<210> 952

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 952

Cys Tyr Leu His His Trp Asn Tyr Leu Ser Phe Leu Lys Gly Gln Ile

214

1 5 10 15

Ile Arg Glu Met Ser Phe His Leu Leu Asn Tyr Arg Thr Ser Asn Ile

20 25 30

Ser His Gln Trp Ile Arg Leu His Phe Gln Lys Ala Leu Glu Ile Lys

35 40 45

His Ile Ser Lys Gly Val His Arg Val Gly

50 55

<210> 953

<211> 103

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 953

Trp Cys Ser Pro Asp Ser Ser Ser Ser Pro Arg Thr Arg Ser Ser Gly

1 5 10 15

Cys Pro Ser Tyr Ala Gly Arg Arg Pro Thr Gln Ser Leu Ser Gln Pro

20 25 30

Gly Trp Cys Pro Leu Ser Arg Pro Ser Trp Pro Pro Leu Pro Ala Thr

35 40 45

Ser Ser Pro Pro Pro Ala Ser Thr Ser Leu Met Thr Asn Thr Gly Cys

50 55 60

Pro Leu Pro Thr Arg Asn Leu Leu Cys Pro Cys Ser Ser Val Thr Gly

65 70 75 80

Thr Ser Thr Arg Ser Lys Gly Met Glu Gly Thr Thr Glu Arg Pro Glu

85 90 95

Pro Arg Ala Ala Arg Gly Pro

100

<210> 954

<211> 64

<212> PROTEIN

<213> Homo sapiens

<400> 954

His Leu Leu Pro Cys Leu Glu Lys Gln Glu Cys His Leu Leu Leu Pro

1 5 10 15

Leu Phe Leu Val Val Leu Glu Ser Leu His Leu Leu His Phe Pro Glu

20 25 30

Ala Leu Ala Phe Leu His Leu His Pro Glu Trp Val Cys Leu His Leu

35 40 45

Pro His Leu Asp Leu Glu Phe Leu Gln Pro Gln Phe Cys His Leu Asp

215

50 55 60

<210> 955

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 955

His Ala Gly Ala Cys Thr Glu Glu Ile Ala Ile Leu Met Arg Leu Thr

1 5 10 15

Ser Pro Asn Ala Ser Val Leu Ala Ala Thr Pro Glu Asn Ile Val Lys

20 25 30

Trp Arg Phe Gln Lys Ala Ser Leu Gln Glu Gln Pro Gln

35 40 45

<210> 956

<211> 41

<212> PROTEIN

<213> Homo sapiens

<400> 956

Trp Ile Gln Leu Asp Phe Asp Phe Arg Leu Met Arg Lys Glu Asn Pro

1 5 10 15

Lys Leu His Thr Trp Cys Ser Trp Arg Ser Leu Lys Lys Lys Asp Ser

20 25 30

Trp His His Ile Glu Gly Gly Phe Gln

35 40

<210> 957

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 957

Ile Gln Leu Asp Phe Asp Phe Arg Glu Trp Leu Met Cys Cys

1 5 10

<210> 958

<211> 78

<212> PROTEIN

<213> Homo sapiens

<400> 958

Ala Asp Phe Thr Glu Pro Gly Gln Ala Ile Ser Pro His Pro Thr Leu

1 5 10 15

Glu Leu Ser Pro Ala Thr Ser Asn Pro His Pro Leu Gly Pro Gln Ile

20 25 30

Trp Pro Pro Gly Leu Pro Ser His Met Leu Pro Ser Pro His Leu

35 40 45

Ala Thr Arg Val Ser Arg Leu Thr Cys Asp Ile Phe Pro Met Glu Glu

50 55 60

216

Ser Trp Asp Leu Gln Leu Ala Ile Met Asp Cys Ser Asp Phe

65 70 75

<210> 959

<211> 74

<212> PROTEIN

<213> Homo sapiens

<400> 959

Arg Trp Leu Pro Gly Gly Gly Ser Pro Trp Thr Ser Arg Arg Gly Pro

1 5 10 15

Pro Trp Ser Ser Thr Thr Pro Cys Thr Gln Gly Val Arg Glu Gly Thr

20 25 30

Ala Ser Gly Lys Thr Arg Gly Glu Ala Met Gly Ser Gly Gln Ala Thr

35 40 45

Val Thr Ser Met Ser Pro Gly Pro Pro Cys Arg Gly Ser Gly Ala Ser

50 55 60

Ser Phe Ile Arg His Thr Gly Asn Pro Pro

65 70

<210> 960

<211> 49

<212> PROTEIN

<213> Homo sapiens

<400> 960

Pro Gln Gln Arg Ala Pro Leu Val Glu Leu Lys Ile Leu Ala Arg Gly

1 5 10 15

Ser His Ser Ser Arg Pro Gly Ser His Pro Arg Arg His Ile Gln Ser

20 25 30

Gln Leu Ser Gln Ala Leu Glu Leu Gly Gln Pro Ser Thr Asp Phe Ile

35 40 45

Leu

<210> 961

<211> 45

<212> PROTEIN

<213> Homo sapiens

<400> 961

Val Gly Pro Lys Lys Gly Ala Arg His Arg Trp Pro Val Pro Ala Pro

1 5 10 15

Leu Leu Ser Ala Ser Ala Ser Thr Ser Leu Pro Ser Pro Ser Ser Cys

20 25 30

Pro His Thr Leu Phe Arg Lys Asn Pro Leu Trp Arg Cys

35 40 45

217

<210> 962

<211> 56

<212> PROTEIN

<213> Homo sapiens

<400> 962

Ala Thr Met Thr His Pro Arg Thr Ala Thr Ser Leu Asp Ile Met Thr

1 5 10 15

Cys Leu Gln Tyr Gly Ile Pro His His Leu His Phe Asp Ala Arg Thr

20 25 30

Val Glu Glu Pro Gly Trp Tyr Gly Arg Gly Gln His Thr Trp Leu Leu

35 40 45

Leu Leu Lys Ala Gly Asp Arg Ala

50 55

<210> 963

<211> 78

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 963

Ala Gln Gly Ser Val Met Ile Ser Ser Pro Ala Ser Ser Gln Arg Trp

1 5 10 15

Pro Pro Pro Arg Trp Met Ser Ser Glu Val Thr Arg Leu Trp Ser Ser

20 25 30

Ser Gly Ala Gly Lys Ser Glu Thr Cys Gly Ser Cys Ala Arg Ser Ile

35 40 45

Ser Gly Arg Gln Ser Pro Ser Pro Ala Ala Cys Trp Met Ala Trp Leu

50 55 60

Arg His Arg Leu Arg Ala Gly Ala Gly Cys Gly Gln Val Pro

65 70 75

<210> 964

<211> 59

<212> PROTEIN

<213> Homo sapiens

<400> 964

Asn Met Arg Glu Phe Ile Leu Gly Arg Asn Arg Thr Asp Val Met Asn

1 5 10 15

Val Val Arg Thr Ser Leu Glu Ile Gln Thr Trp Gln Ile Ile Thr Glu

20 25 30

Ser Ile Leu Glu Arg Asn Arg Thr Asp Glu Met Cys Val Val Arg Ser

35 40 45

218

Leu Val Ile Ile His Thr Leu His Ser Met Arg

50 55

<210> 965

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 965

Leu Glu Ile Arg Gln Leu Ser Ser Lys Ser Leu Trp Arg Val Gly Pro

1 5 10 15

Lys Thr Arg Val Leu Asn Ser Ser Lys Pro Pro Leu Ser Gln Ile Pro

20 25 30

Leu Arg

<210> 966

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 966

Leu Trp Thr Ile Arg Asn Tyr Leu Leu His Lys Leu Leu Lys Leu Phe

1 5 10 15

Arg Ile Tyr Asn Ser Gly Asp Phe

20

<210> 967

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 967

Ser Gln Gly Ser Gln Asn Phe Ala Leu Thr Lys His His Trp Leu Ile

1 5 10 15

Leu Glu Ser Ile Cys Ala Lys

20

<210> 968

<211> 59

<212> PROTEIN

<213> Homo sapiens

<400> 968

His Pro Thr Thr Trp Asn Ala Cys Pro Trp Gly Gln Leu Pro Thr Gly

1 5 10 15

Glu Gln Ile Arg Pro Gly Arg His Pro Pro Leu Arg Pro Gln Arg Pro

20 25 30

Ser Pro Arg Pro Arg Leu Leu Thr Met Pro Thr Gly Leu Gln Ser Arg

35 40 45

219

Leu Phe Ser Ala Glu Lys Thr Pro Leu Pro Leu

50 55

<210> 969

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 969

Asn Ser Pro Phe Gly Ser Ser Cys Leu Gln Thr Ser Asn Asn Asn Met

1 5 10 15

Glu Lys Val Thr Leu Lys Lys Val

20

<210> 970

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 970

Ile Ser Phe Arg Val Leu Ala Ser Ser Trp Tyr Ala Ser Leu Pro Ser

1 5 10 15

Pro Ser Cys Asn Leu Trp Met Pro Ser Thr Lys Ile Arg Leu Phe Thr

20 25 30

Ala Ile

<210> 971

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 971

Ser Leu Phe Leu Pro Gly Ser Thr Pro Ser Leu His Phe Leu Cys Trp

1 5 10 15

Ser Leu Arg Ser Ser Gly Gly Cys Arg Ser Ala

20 25

<210> 972

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 972

Leu Glu Ser Leu Pro Ala Phe Leu Arg Cys His Leu Lys Thr Glu Val

1 5 10 15

Pro Cys Gly Leu Trp Leu Arg Ser Met Gly Glu Ile Ala Ser

20 25 30

<210> 973

<211> 38

<212> PROTEIN

<213> Homo sapiens

220

<400> 973

Val Gly Glu Leu Cys Gly Gly Ser Glu Pro Gln Thr Gln Ser Trp Cys

1 5 10 15

Val Gln Leu Gly Val Gly Met Gly Leu Lys Lys Pro Ser Cys Trp Cys

20 25 30

Trp Thr Leu Met Trp Thr

35

<210> 974

<211> 35

<212> PROTEIN

<213> Homo sapiens

<400> 974

Arg Arg Arg Thr Pro Thr Ala Met Gly Arg Asn His Ser Cys Thr Ser

1 5 10 15

Arg Ser Pro Ser Ser Ser Trp Met Ser Ser Glu Ser Trp Arg Ser Arg

20 25 30

Thr Cys Arg

35

<210> 975

<211> 30

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 975

His Trp Ser Pro Ser Pro Leu His His His Ala His Cys Leu Pro Thr

1 5 10 15

Pro Glu Trp Pro Arg Ala Trp Val Leu Gln Gln Tyr Pro His

20 25 30

<210> 976

<211> 20

<212> PROTEIN

<213> Homo sapiens

<400> 976

His His His Arg Arg Ala Pro Gly Gln Gly Ser Ala Thr Ala Ser Pro

1 5 10 15

Ala Arg Cys Thr

20

<210> 977

<211> 29

<212> PROTEIN

<213> Homo sapiens

<400> 977

221

Trp Ser Arg Ser Trp Arg Ser Ser Arg Cys Pro Cys Cys Gly Trp Ser

1 5 10 15

Thr Thr Ala Arg Pro Ala Cys Ser Met Arg Leu Thr Ser

20 25

<210> 978

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 978

Asn Gly Asn Pro Val Gln Glu Ser Pro Gln His Gln Met Ile Gln Asp

1 5 10 15

Ala Gln Gly

<210> 979

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 979

Cys Pro Met Met Asn Phe Leu Arg Tyr Tyr Leu Arg Leu Lys Ile Pro

1 5 10 15

Leu Gly Cys Asn Leu Thr

20

<210> 980

<211> 26

<212> PROTEIN

<213> Homo sapiens

<400> 980

Leu Thr Lys Leu Met Val Trp Leu Gln Tyr Gly Gln Ala Gly Lys Ile

1 5 10 15

Arg Phe Thr Val Leu Leu Phe Pro Pro Cys

20 25

<210> 981

<211> 20

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 981

Ala Ser Thr Ser Ser Val Thr Ala Ser Cys Ala Ser Thr Thr Ser Arg

1 5 10 15

Gly Met Trp Asn

20

<210> 982

<211> 27

222

<212> PROTEIN

<213> Homo sapiens

<400> 982

Leu Ala Arg Ser Ser Leu Ser Leu Glu Ala Asn Ser Pro Tyr Leu Gly

1 5 10 15

Val Trp Arg Pro Pro Leu Arg Met Gly Leu Trp

20 25

<210> 983

<211> 27

<212> PROTEIN

<213> Homo sapiens

<400> 983

Leu Gly Gly Gly Cys Ile Thr Leu Ala His Val Cys Gln Ala Trp Pro

1 5 10 15

Val Tyr Gly Leu His Leu Thr Asp Leu Phe Val

20 25

<210> 984

<211> 18

<212> PROTEIN

<213> Homo sapiens

<400> 984

Leu Ser Arg Asn Ser Thr Leu Leu Ile Glu Glu Asn Leu His His Ser

1 5 10 15

Lys Asn

<210> 985

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 985

Asn Thr Gly Pro Arg Lys Asn Arg Ser Gly Lys Lys Trp Lys

1 5 10

<210> 986

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 986

Pro Pro Val Trp Ser Arg Cys Gly Arg Arg Cys

1 5 10

<210> 987

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 987

Leu Ser Leu Pro Glu Thr Ala Leu Lys Gln Tyr Ala Cys Thr Val

223

1 5 10 15

<210> 988

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 988

Gln Leu Asp Leu Leu Leu Pro Ser Glu Tyr Tyr Met Phe Pro Ala Trp

1 5 10 15

Gly Ser Val Phe Trp

20

<210> 989

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 989

Ser Met Lys Lys Ile Glu Ser Trp Thr Ser Leu His Pro

1 5 10

<210> 990

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 990

Ser Leu Phe Arg Glu Thr Gln Ser Asn Arg

1 5 10

<210> 991

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 991

Leu Thr Ser Ser Cys Gly Ser Trp Ile Ala Tyr Cys Cys Cys Phe Lys

1 5 10 15

Gly Lys Ser Val Leu

20

<210> 992

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 992

Pro Phe Leu Pro Ile Gln Lys Ala Ala Ser Gly Arg Gly

1 5 10

<210> 993

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 993

224

Ser Asp Ala Pro Leu Glu Ala Leu Thr Gly Gln Gly Met

1 5 10

<210> 994

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 994

Lys Lys Lys Lys Arg Thr Arg Ile Ile Met

1 5 10

<210> 995

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 995

Arg Met Lys Lys Val Ile Ile Lys Trp Ala

1 5 10

<210> 996

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 996

Ile Asn Glu Lys Arg Leu Thr Cys Ser Ile Leu

1 5 10

<210> 997

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 997

Ala Thr Ser Thr Pro Gly Arg Leu Ser Ser Ser Pro Arg Cys Pro Gly

1 5 10 15

Asn Gly Gly

<210> 998

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 998

Asn Val Gly Arg Pro Leu Asp Thr Val Gln Pro Leu Lys Cys Ile

1 5 10 15

<210> 999

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 999

Asp Glu Lys Lys Arg Glu Lys Val Ile Ser

1 5 10

225

<210> 1000

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1000

Ser Asn Lys Lys Trp Lys Lys Met Lys Arg Ser

1 5 10

<210> 1001

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 1001

Thr Met Lys Lys Ile Val Met Tyr

15

<210> 1002

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 1002

Gly Gly Gly Thr Gln Asp Ser Val Trp Gln Ala Cys Arg Lys Ser Gln

1 5 10 15

Val Pro Leu Leu Ser

20

<210> 1003

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 1003

Phe Ser Pro Pro Pro Ala Ala Cys Ser Pro Ala Lys

1 5 10

<210> 1004

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1004

Thr Val Gln Lys Met Gln Asn Thr Cys Val

1 5 10

<210> 1005

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1005

Asn Pro Lys Lys Asp Gln Leu Leu Lys Asp Phe

1 5 10

<210> 1006

<211> 10

226

<212> PROTEIN

<213> Homo sapiens

<400> 1006

Arg Met Gly Gly Ser Gln Leu Ser Arg Ile

1 5 10

<210> 1007

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1007

Thr Asn Lys Lys Gln Leu Met Arg Lys Lys Ser

1 5 10

<210> 1008

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1008

Glu Leu Lys Lys Ile Thr Val Asn Tyr Leu Gln

1 5 10

<210> 1009

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1009

Phe Ile Glu Lys Asn Ser Lys Met Thr Gln Ile Ile Gln

1 5 10

<210> 1010

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1010

Glu Ala Lys Glu Val Phe Pro Lys Met Leu

1 5 10

<210> 1011

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1011

Arg Val Leu Ala Thr His Pro Gln Ala Thr

1 5 10

<210> 1012

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 1012

Ala Ala Gly Gly Thr Gly Arg Arg

15

227

<210> 1013

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1013

Ala Gln Pro Gly Ser Gly Leu Glu Thr Cys

1 5 10

<210> 1014

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1014

Lys Gly Lys Lys Ala Ile Gln Arg Gln Ser Gln Met Arg

1 5 10

<210> 1015

<211> 37

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1015

Cys Trp Ser Pro Leu Gln Pro Arg Ala Arg Gln Arg Gln Thr Arg Cys

1 5 10 15

Gln Val Arg Pro Pro Pro Gly Pro Pro Cys Leu Pro Leu Ala Lys Arg

20 25 30

Ser Arg Ser Pro Arg

35

<210> 1016

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 1016

Arg Arg Ser Cys Leu Pro His Gln Leu His Pro Trp Gly Pro Ser Cys

1 5 10 15

Leu Leu Cys Leu Gly Thr Ile Val Pro Leu Pro Cys Ala Pro Ser Ser

20 25 30

ProGly

<210> 1017

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1017

Pro Gly Ala Leu Leu Asn Pro Ser Arg Gly

228

1 5 10

<210> 1018

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1018

Cys His Pro His His Pro Gln Val Pro His His Pro Leu

1 5 10

<210> 1019

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1019

Leu Gly Leu Asn Met Lys Arg Asn Ser Lys Pro

1 5 10

<210> 1020

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1020

Glu Pro Lys Lys Ser Leu Ile Pro Leu Ser Gly

1 5 10

<210> 1021

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 1021

Gly Trp His Trp Ala Phe Arg Lys Glu

15

<210> 1022

<211> 49

<212> PROTEIN

<213> Homo sapiens

<400> 1022

Gly Lys Thr Ser Thr Pro Leu Thr Ser Ile Ser Pro Arg Trp Pro Ser

1 5 10 15

Leu Leu Thr Cys Ser Trp Leu Gly Trp His Trp Ala Phe Arg Lys Gly

20 25 30

Ser Pro Arg Arg Cys Trp Ala Cys Val Gln Ala Gln Arg Trp Cys Gly

35 40 45

trp

<210> 1023

<211> 10

<212> PROTEIN

229

<213> Homo sapiens

<400> 1023

Val Asn Phe Val Lys Ile Leu Met Ile Gln

1 5 10

<210> 1024

<211> 38

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1024

Gly Ser Gly Ser Ala Pro Cys Arg Arg Val Arg Gly Pro Ala Ser Pro

1 5 10 15

Met Gly Met Arg Arg Arg Ala Gln Pro Ser Ala Ser Thr Arg Arg Cys

20 25 30

Ser Ala Cys Ser Thr Ser

35

<210> 1025

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 1025

Ala Leu Ser Val Tyr Gln Phe Phe Ile Pro Gln Arg Glu Val Lys Ile

1 5 10 15

Ser Thr Ile Ile Leu Pro Ser Asn Lys Pro Phe Lys Pro Leu Thr Lys

20 25 30

Arg Ile Leu Val Leu Leu Gly Ser Ile Lys

35 40

<210> 1026

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 1026

Ala Arg Pro Ala Arg Pro Cys Pro Thr Gly His Ser Leu Val Gln Met

1 5 10 15

Glu Pro Leu Thr Thr Ala Arg Pro Thr Ser Ser Ser Thr Thr Cys Pro

20 25 30

Arg Thr

<210> 1027

<211> 18

<212> PROTEIN

<213> Homo sapiens

230

<400> 1027

Ala Thr Arg Met Pro Glu Pro His Arg Ala Leu Pro Thr Arg Val Pro

1 5 10 15

PhePro

<210> 1028

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1028

Ser Leu Val Met Lys Gly Gln Ile Pro Val Arg

1 5 10

<210> 1029

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1029

Tyr Tyr Lys Tyr Val Thr Asn Ala Cys Leu

1 5 10

<210> 1030

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1030

Ser Leu Pro Pro Ala Pro Leu Leu His Glu

1 5 10

<210> 1031

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 1031

Ile Leu Thr Met Arg Gln Trp Leu Lys Asn Arg Ser Leu Glu Arg Thr

1 5 10 15

His Leu Leu Ile Ile Gln Asn Ile

20

<210> 1032

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 1032

Thr Pro Trp Ser Thr Thr Arg Pro Thr Thr Pro Trp Leu Thr

1 5 10

<210> 1033

<211> 11

<212> PROTEIN

<213> Homo sapiens

231

<400> 1033

Leu Thr Ile Lys Ile Thr Ser Thr Phe Ile Ser

1 5 10

<210> 1034

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 1034

Arg Arg Pro Cys Thr His Ser Gly Ser Thr Thr Arg Ser His Ser

1 5 10 15

<210> 1035

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1035

Val Ile Glu Lys Asn Leu Met Val Gly Gly

1 5 10

<210> 1036

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1036

Thr Ala Glu Lys Arg Ile Lys Met Ser Thr

1 5 10

<210> 1037

<211> 55

<212> PROTEIN

<213> Homo sapiens

<400> 1037

Thr Pro Met Asp Ser His Arg Ser Ser Gln Asp Lys Thr Leu Thr His

1 5 10 15

Pro Ser Met Glu Thr Thr Arg Arg Arg Val Pro His Pro Thr Met Thr

20 25 30

Thr Arg Thr Ser Leu Pro Pro Thr Gly Met Thr Arg Ala Ser Asp Arg

35 40 45

Pro Ser Ser Ala Arg Cys Ser

50 55

<210> 1038

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 1038

Leu Ser Thr Val Thr Thr His Trp Ser Ser Gly Trp Thr Leu Cys Trp

1 5 10 15

232

Ala Leu Pro Lys Arg Ala Ser Asn Trp Thr Ser Arg Thr Ser Arg Gln

20 25 30

Trp Ala Pro Pro Trp Thr Ser Cys Gly Ser

35 40

<210> 1039

<211> 39

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1039

Leu Pro Gln Pro Cys Gly Pro Ala Ser Cys Ser Thr Pro Ser Trp Pro

1 5 10 15

Met Ala Val Pro Thr Ser Arg Ser Cys Met Ala Arg Ser Pro Arg Pro

20 25 30

Ser Ala Cys Gln Leu Pro Arg

35

<210> 1040

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 1040

Val Val Asn Leu Gly His Ser Cys Cys Leu Asp Pro Glu Thr Arg Leu

1 5 10 15

Leu Arg Cys Gly Met Ser Val Leu Ala Cys Ala Leu

20 25

<210> 1041

<211> 23

<212> PROTEIN

<213> Homo sapiens

<400> 1041

Leu Leu His Phe Leu Glu Lys Ser Pro Leu Glu Glu Trp Asn Gln Trp

1 5 10 15

Val Met His Leu Cys Trp His

20

<210> 1042

<211> 26

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1042

Arg Ile Ser Leu Phe Leu Gly Ser His Pro Phe Arg Ala Arg Ile Leu

1 5 10 15

233

Cys Leu Thr Pro Cys Lys Pro Arg Ser Ser

20 25

<210> 1043

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 1043

Asn Leu Trp Leu Ile Val Leu Thr Gln Leu Val Leu Asn Leu Val Gln

1 5 10 15

Glu

<210> 1044

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 1044

Pro Thr Thr Gln His Met Thr Thr Ser Ala Gly Ser Leu Ser Pro Thr

1 5 10 15

<210> 1045

<211> 16

<212> PROTEIN

<213> Homo sapiens

<400> 1045

Leu Leu Lys Arg Thr Ile Gln Met Pro Pro Ala Ser Pro Ala Ser Ser

1 5 10 15

<210> 1046

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1046

Arg Glu Arg Phe Leu Ser Asn Arg Met Lys Trp Lys Tyr

1 5 10

<210> 1047

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1047

Lys Pro Ile Phe Leu Asn Arg Tyr Leu Leu Gln

1 5 10

<210> 1048

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1048

His Asn Pro Phe Leu Ala Ser Cys Arg Trp

234

1 5 10

<210> 1049

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1049

Gln Cys Pro Ser Cys Met Pro Ser Gly Thr

1 5 10

<210> 1050

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 1050

Glu Thr Ala Pro His Leu His Leu Leu

15

<210> 1051

<211> 58

<212> PROTEIN

<213> Homo sapiens

<400> 1051

Pro Gln Trp Cys Gly Trp Val Arg Tyr Glu Gly Pro Arg Ala Glu Pro

1 5 10 15

Trp Ala Lys Glu His Pro Leu Val Leu Thr Leu Gln Pro Gln Ala Gly

20 25 30

Thr Leu Tyr Ile Phe Thr Asp Lys Ser Lys Asn Leu Tyr Ser Leu Cys

35 40 45

Gly Ser Thr Leu Val Ser Leu Gln Val Ser

50 55

<210> 1052

<211> 74

<212> PROTEIN

<213> Homo sapiens

<400> 1052

Cys Ser Gln Arg Arg Trp Ile His Leu Met Arg Tyr Ala Thr Ala Phe

1 5 10 15

Leu Cys Asn Ser Val Asp Asn Met Ile Ser Gln Cys Leu Gln Phe Glu

20 25 30

Cys Phe Leu Lys Cys Arg Lys Leu Val Leu Thr Pro Met Pro Leu Leu

35 40 45

Met Val Ile Ile Ile Arg Leu Phe Trp Lys Val Pro Gly Leu Gln Glu

50 55 60

Val Val Val Ala Ile Phe Phe Gly Gln Lys

65 70

235

<210> 1053

<211> 53

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1053

Arg Gly Ala Ala Leu Ala Ile Ser Met Gly Val Arg Val Met Trp Ala

1 5 10 15

Ser Phe Lys His Ser Thr Leu Ala Leu Ala Val Cys His Ile Ile Ser

20 25 30

Arg Ile Thr Leu Gly Leu Ser Leu Gln Gly Val Leu Ser Thr Asn Ser

35 40 45

Ser Lys Ser Ser Ser

50

<210> 1054

<211> 84

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1054

Arg Arg Ser Cys Pro Thr Leu Arg Ala Cys Ala Asn Ser Trp Ser Thr

1 5 10 15

Trp Leu Ser Leu Trp Ser His Arg Pro Pro Pro Gly Ala Ser Ser Gly

20 25 30

Trp Ala Gly Pro Ala Ala Thr Ser Ser Leu Glu Ala Ser Gly Met Pro

35 40 45

Pro Ser Ser Pro Ser Met Ala Ser Leu Cys Trp Ser Thr Val Ala Gln

50 55 60

Thr Pro Ser Pro Val Ser Gly Ser Trp Cys Gly Thr Trp Thr Ala Trp

65 70 75 80

Met Pro Cys Trp

<210> 1055

<211> 54

<212> PROTEIN

<213> Homo sapiens

<400> 1055

Glu Lys Ser Val Lys Glu Ile Lys Cys Phe Leu Thr Met Asn Leu Asn

1 5 10 15

236

Ile Ala Lys Val Leu Leu Lys Thr Lys Val Tyr Phe Met Tyr Leu Thr

20 25 30

Ser Leu Ser Lys Asn Pro Lys Ile Phe Met His Arg Asn Leu Lys Gln

35 40 45

Lys Trp His Leu Gly Ile

50

<210> 1056

<211> 51

<212> PROTEIN

<213> Homo sapiens

<400> 1056

Arg Arg Lys Ser Trp Lys Met Lys Ser Pro Val Asp Thr Val Leu Ser

1 5 10 15

Lys Glu Asn Gln Ile Cys Lys Val Lys Thr Ile Leu Ala Pro Ala Pro

20 25 30

His Ala Leu Ser Leu Pro Arg Pro Gln Pro Phe Phe Arg Ala Ile Asp

35 40 45

Lys Asp Arg

50

<210> 1057

<211> 41

<212> PROTEIN

<213> Homo sapiens

<400> 1057

Ser Arg Leu Ser Pro Pro Glu Ala Ala Phe Gln Arg Met Leu Ala Met

1 5 10 15

Glu Leu Arg Ser Pro Asp His Pro Arg Ala Glu Tyr Pro Asp Phe Ser

20 25 30

Pro Ser Pro Gly Pro Val Ala Gly Leu

35 40

<210> 1058

<211> 38

<212> PROTEIN

<213> Homo sapiens

<400> 1058

Pro Arg Pro Leu Pro Gly Leu Pro Ala Pro Pro Gln Arg Ser Ser Ser Trp

1 5 10 15

Met Gly Ser Ser Cys Ser Ser Cys Gly Thr Arg Pro Met Ser Ser Trp

20 25 30

Leu Cys Ala Trp Gly Glu

35

237

<210> 1059

<211> 15

<212> PROTEIN

<213> Homo sapiens

<400> 1059

Ser Trp Met Gly Ser Ser Cys Ser Cys Gly Thr Arg Pro Met Ser

1 5 10 15

<210> 1060

<211> 34

<212> PROTEIN

<213> Homo sapiens

<400> 1060

Asn Trp Ile Leu Val Asn Leu Phe Ser Gly Thr Ser Lys Ala Pro Phe

1 5 10 15

Leu Cys Gly Gly Ile Asp Gln Thr Ser Gln Pro Ser Ala Gly Pro Arg

20 25 30

Gly Thr

<210> 1061

<211> 28

<212> PROTEIN

<213> Homo sapiens

<400> 1061

Val Gly Arg Ser Gly Trp Gly Val Pro Ala Ser Cys Thr Ala Ala Pro

1 5 10 15

Pro Thr Ser Arg Trp Met Ser Trp Gln Asp Cys Ser

20 25

<210> 1062

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 1062

Ser Ala Gly Val Leu Arg Gly Ser Lys Arg Thr Gly Pro Ala Cys Met

1 5 10 15

Pro Thr Lys Arg Arg Thr Val Met Lys Ser Thr Trp Arg Ser

20 25 30

<210> 1063

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 1063

Ile Trp Trp Ile Val Gln Asn Ser Phe Leu Leu Pro Phe Arg Met Ser

1 5 10 15

Ser Phe Pro Lys Lys Phe Phe Phe Leu

20 25

238

<210> 1064

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 1064

Ala Asn Ser Lys Leu Ser Ser Ser Leu Leu Leu Arg Lys His Ser Cys

1 5 10 15

Gly Gln Lys His Leu Thr Ser Cys

20

<210> 1065

<211> 21

<212> PROTEIN

<213> Homo sapiens

<400> 1065

Ile Ser Ser Trp Gln Val Leu Phe Met Asn Ile Gln Glu Asp Ile Leu

1 5 10 15

Ser Leu Leu Ser Gln

20

<210> 1066

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 1066

Leu Glu Trp Gln Ala Leu Asp Tyr Ser Arg Ser Trp Leu Trp Thr Asp

1 5 10 15

Thr

<210> 1067

<211> 12

<212> PROTEIN

<213> Homo sapiens

<400> 1067

Leu Leu Arg Gln Gly Lys Cys Gln Ser Ala Trp Ser

1 5 10

<210> 1068

<211> 83

<212> PROTEIN

<213> Homo sapiens

<400> 1068

Pro Arg Ser Tyr Met Pro Thr Leu Cys Ala Ala Ser Thr Met Pro Trp

1 5 10 15

Gln Lys Thr Phe Pro Ser Asn His Trp Cys Arg Trp Gln Pro Gly Ala

20 25 30

239

Leu Gly Ser Met Gly Thr Ser Cys Trp Gln Gly Thr Ala Arg Arg Leu

35 40 45

Ser Pro Phe Arg Trp Thr Lys Arg Lys Cys Trp His Cys Trp Lys Arg

50 55 60

Cys Cys Ser Pro Thr Cys Pro Cys Gln Pro Leu Glu Asp Met Pro Ser

65 70 75 80

Gln Pro Ser

<210> 1069

<211> 46

<212> PROTEIN

<213> Homo sapiens

<400> 1069

Gln Leu Ala Leu Lys His Gln Leu Arg His Pro Leu His Pro Pro His

1 5 10 15

Leu Val Pro Lys Leu Gln Arg Arg Gln Phe Leu Ala Lys Gln Gly Gln

20 25 30

Trp Gly Pro Pro Arg Ala Arg Glu Lys Leu Thr Ser Ser Gln

35 40 45

<210> 1070

<211> 61

<212> PROTEIN

<213> Homo sapiens

<400> 1070

His Pro Val Arg Pro Pro Pro Asn Val Arg Ala Trp Val Gly Gln

1 5 10 15

Gln Pro Pro Met Pro Leu Gly Arg Pro Leu Gly Leu Glu Gly Gln Gly

20 25 30

Ala Arg Leu Ile Pro Val Ser Leu Lys Gly Phe Trp Asp Pro Arg

35 40 45

Pro Thr Gly Lys Gly Leu Glu Gly Gly Arg His Arg Thr

50 55 60

<210> 1071

<211> 42

<212> PROTEIN

<213> Homo sapiens

<400> 1071

Ala Ser Pro Arg Arg Pro Arg Arg Ala Leu Arg Gly Pro Val Arg Ala

1 5 10 15

Arg Thr Pro Arg Gly Ser Ser Ser Met Arg Ser Leu Ser Met Thr Ser

20 25 30

240

Leu His Ser Ala Leu Thr Gln Ser Leu Ser

35 40

<210> 1072

<211> 57

<212> PROTEIN

<213> Homo sapiens

<400> 1072

Glu Ala Pro Ser Thr Arg Ala Ala Pro Pro Ser Leu Arg Ala Ala Arg

1 5 10 15

Ala His Gln Arg Thr Gly Arg Gln His Pro Pro Pro Ala Lys His Ser

20 25 30

Ser Ser Gln Cys Thr Gln Gly Arg Arg Trp Gly Ala Trp His Pro Gly

35 40 45

Ser Trp Pro Val Ala Gln Arg Met Gly

50 55

<210> 1073

<211> 30

<212> PROTEIN

<213> Homo sapiens

<400> 1073

Arg Pro Trp Asn Ser Ser Thr Arg Gly Cys Cys Arg Pro Val Gly Arg

1 5 10 15

Pro Arg Pro Arg Gly Gly Trp Val Trp Trp Ala Pro Arg Ala

20 25 30

<210> 1074

<211> 25

<212> PROTEIN

<213> Homo sapiens

<400> 1074

Leu Thr Pro Val Glu Lys Leu Arg Arg Lys Ile Ser Glu Leu Leu Leu

1 5 10 15

Gly Asn Asn Met Gln Pro Asn Ser Ile

20 25

<210> 1075

<211> 22

<212> PROTEIN

<213> Homo sapiens

<400> 1075

Leu Ala Pro Pro Asp Trp Pro Gly Ile Trp Met Asp Ser Cys Arg Arg

1 5 10 15

Pro Ala Pro Gln Asp Cys

20

<210> 1076

241

<211> 25

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Modified FSP Homo sapiens

<400> 1076

Arg Pro Cys Ser Ser Cys Trp Gly Leu Val Leu Pro Thr Cys Leu Leu

1 5 10 15

Ala Ser Trp Gln Pro Gly Gly Cys Arg

20 25

<210> 1077

<211> 24

<212> PROTEIN

<213> Homo sapiens

<400> 1077

Ala Thr Pro Val Ala Asn Val Ser Ala Gly Ala Pro Thr Ser Ile Ala

1 5 10 15

Thr Ser Leu Phe Thr Ile Pro Ser

20

<210> 1078

<211> 17

<212> PROTEIN

<213> Homo sapiens

<400> 1078

Arg Pro Ala Ser Ser Trp Cys Ser Pro Ser Arg Ser Val Pro Ser Ala

1 5 10 15

Ser

<210> 1079

<211> 19

<212> PROTEIN

<213> Homo sapiens

<400> 1079

Arg Ser Ile Phe Arg Gly His Arg Ser Trp Asp Leu Pro Ile Ile Val

1 5 10 15

Asn Leu Ser

<210> 1080

<211> 10

<212> PROTEIN

<213> Homo sapiens

<400> 1080

Glu Ser Glu Lys Asn Trp Trp Lys Asn Leu

1 5 10

242

<210> 1081

<211> 14

<212> PROTEIN

<213> Homo sapiens

<400> 1081

Asp Ile Thr Ile Thr Leu Glu Leu Arg Thr Ile Trp Ile Val

1 5 10

<210> 1082

<211> 11

<212> PROTEIN

<213> Homo sapiens

<400> 1082

Pro Gln Pro Pro Gly Cys Cys Pro Trp Pro Ala

1 5 10

<210> 1083

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1083

Arg Ser Phe Phe Pro Lys Ser Ser Pro Leu Phe Arg Met

1 5 10

<210> 1084

<211> 13

<212> PROTEIN

<213> Homo sapiens

<400> 1084

Leu Leu Gly Gly Cys Ser Gly Leu Pro Trp Pro Arg Arg

1 5 10

<210> 1085

<211> 9

<212> PROTEIN

<213> Homo sapiens

<400> 1085

Arg Cys Pro Pro Arg Trp Ser Pro Cys

15

<210> 1086

<211> 8

<212> PROTEIN

<213> Homo sapiens

<400> 1086

Thr Leu Arg Phe Leu His Leu Ser

15

<210> 1087

<211> 83

<212> PROTEIN

<213> Homo sapiens

<400> 1087

243

His Pro Gln Val Cys Pro Leu Arg Gly Ser Gln Leu Gln Arg Thr Glu

1 5 10 15

Gln Gly Glu Asp His Pro Leu His Pro Leu Ser Arg Gln His Arg Ala

20 25 30

Leu Val Val Gly Glu Leu Gly Pro Gln Ala Trp Pro Gln Leu Leu Leu

35 40 45

Glu Pro Asn Ser Gly Lys Ser Ala Ser Arg Arg Arg Pro Gln Gly Gly

50 55 60

Pro Gln Pro Pro Lys Leu Arg Val Val Glu Ala Glu Val Gly Asp Ser

65 70 75 80

Trp Lys Arg

<210> 1088

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 1A polypeptide

<400> 1088

Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser

1 5 10 15

Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro

20 25 30

Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala Leu Ala Lys Lys Thr

35 40 45

Leu Thr Asn Ala Ser Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr

50 55 60

Ser Arg Gly Met Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro

65 70 75 80

Leu Arg Cys Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn

85 90 95

Gly Trp His Arg Ser Gly Leu Arg Ala Gln Met Pro Ser Ser Trp Ser

100 105 110

Ser Ser Gly Arg Thr Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr

115 120 125

Leu Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Arg Ser Leu

130 135 140

Leu Tyr Ser Arg Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu

145 150 155 160

244

Pro Asp Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln

165 170 175

Ser Phe Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys

180 185 190

Cys Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Asn Gln Gln

195 200 205

Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser Phe Asp Lys Lys

210 215 220

Gly Phe Val Ile Leu Ser Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile

225 230 235 240

Gly Ser Ile Ala Ser Ser Pro Gly His Pro Leu Gly Pro Thr Leu Ala

245 250 255

Ile Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala

260 265 270

Ala Pro Ser Gly Gln Tyr Phe Ile Ala Lys Lys Arg Ile Lys Leu Arg

275 280 285

Arg Asn Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr Arg Lys Arg

290 295 300

Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu Gln Leu

305 310 315 320

Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg Ser Pro

325 330 335

Trp Ile Phe Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu

340 345 350

Ala Ile Thr Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys

355 360 365

His Pro His Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe

370 375 380

Ser His His Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu

385 390 395 400

Arg Gly Gly Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Gln Arg Lys

405 410 415

Val Val Lys Asn Pro Ser Gln Pro Val Pro Leu Arg Asn Asp Gly Ile

420 425 430

Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser Leu Lys Lys

435 440 445

245

Ser Leu Glu Lys Met Pro Leu Met Arg Arg Ala Arg Pro Thr Ser Cys

450 455 460

Ser Thr Cys Arg Met Trp Trp Lys Gly Lys Lys Arg Arg Lys Glu Ile

465 470 475 480

Val Pro Met Ser Leu Ala Cys Ser Ile Thr Val Ser Phe Val Gly Gln

485 490 495

Ile Ser Thr Leu Val Leu Asp Val Ser Ser Ile Ser His Trp Arg Leu

500 505 510

Pro Cys Lys Val Thr Glu Ser Ser Ser Ser Phe Phe Tyr Thr Ser Asp

515 520 525

Arg Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln Ser

530 535 540

Cys Cys Leu Pro Gly Leu Thr His Pro Ala His Gln Pro Leu Gly Ser

545 550 555 560

Met Ser Thr Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro

565 570 575

Gly Pro Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser

580 585 590

Lys Leu Arg Leu Arg Asn Thr Ser Pro Gly Glu Lys Asn Asp Leu Gln

595 600 605

Leu Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile

610 615 620

Leu Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr Thr Ser Leu Gln

625 630 635 640

Arg Pro Pro Arg Ser Ile Leu Ser Leu Asn Gly Ile Trp Glu Val Met

645 650 655

Pro Arg Thr Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr Ser

660 665 670

Gln Asn His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu Asn

675 680 685

Val Ser Lys Lys Arg Leu Pro Lys Leu Met Lys Lys Lys Asp Ser Thr

690 695 700

Ile Phe Ile Asn Lys Val Arg Gln Asn Phe Lys Arg Asn Phe Lys Lys

705 710 715 720

Lys Lys Pro Leu Thr Phe Ser Pro Ser Arg Arg Met Thr Arg Arg Thr

725 730 735

Met Thr Thr Thr Lys Met Met Met Met Arg Arg Thr Lys Arg Arg Arg

246

740 745 750

Arg Lys Arg Arg Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr

755 760 765

Lys Lys Thr Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu

770 775 780

Pro Arg Gln Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly

785 790 795 800

Thr Ser Leu Ala Ile Trp Gly Arg Thr Arg Leu Thr Leu Thr Gly Thr

805 810 815

Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys

820 825 830

Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu

835 840 845

Arg Ala Pro Val Gly Ala Gln Ala Pro Pro Glu Val Thr Leu Arg Ala

850 855 860

Ser Cys Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp

865 870 875 880

Gly Ala Glyn Val Arg Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys

885 890 895

Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala

900 905 910

Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro

915 920 925

Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg

930 935 940

Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu

945 950 955 960

Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro

965 970 975

Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro

980 985 990

Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu

995 1000 1005

Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro Ser Cys

1010 1015 1020

His Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln Ala

1025 1030 1035

247

Pro Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe Arg

1040 1045 1050

Asn Tyr Pro Arg Ala Asp Lys Lys Asn Val Arg Ser Thr Ala Phe

1055 1060 1065

Phe Leu Leu Asp Ile Ile Leu Gly Asp Cys Pro Arg Thr Met Ala

1070 1075 1080

Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser Arg

1085 1090 1095

Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro

1100 1105 1110

Ala Gly Trp Ala Arg Gln Ala Tyr Pro Arg Leu Lys Thr Phe Gly

1115 1120 1125

Val Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr

1130 1135 1140

Met Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala

1145 1150 1155

Ser Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro

1160 1165 1170

Trp Arg Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro

1175 1180 1185

Thr Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg

1190 1195 1200

Met Pro Gly Ser Gln Ser Ile Met Met Ser Trp Met Pro Pro Leu

1205 1210 1215

Ala Pro Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys

1220 1225 1230

Pro Ala His Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr

1235 1240 1245

Pro Leu Ser Ala His Pro Val Pro Val Leu Ser Gly Cys His Leu

1250 1255 1260

Ala Val Arg Thr Ser Met Leu Thr Leu Leu Pro Met Glu Ala Lys

1265 1270 1275

Lys Ser Met Lys Arg Ser Asn Ala Lys Lys Met Glu Asp Met Ile

1280 1285 1290

Trp Glu Ser Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro

1295 1300 1305

248

Leu His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg

1310 1315 1320

Ile Val Phe Phe Leu Ala Pro Tyr Phe Pro Arg His Pro His Arg

1325 1330 1335

Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu

1340 1345 1350

Pro Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His Ser Ser Val

1355 1360 1365

Leu Ala Thr Gly Leu His Leu His Arg Gln Ile Ser Leu Pro Leu

1370 1375 1380

Arg Gly Phe Leu Leu His Gln Pro Leu Pro Gly Gln His Leu Trp

1385 1390 1395

Leu Ser His Arg Leu Arg Leu Arg Leu Pro Arg Thr Gln Pro Ser

1400 1405 1410

Leu Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro

1415 1420 1425

Pro Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala Thr Leu Arg

1430 1435 1440

Cys Pro Ala Val Pro Ala Ala Ala Cys Pro Pro Ala Leu Arg Ser

1445 1450 1455

Met Pro Lys Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu Thr

1460 1465 1470

Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro

1475 1480 1485

Gln Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln

1490 1495 1500

Ser Met Leu Val

1505

<210> 1089

<211> 1501

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 2A polypeptide

<400> 1089

Arg Lys Met Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Gly Ser

1 5 10 15

Pro Pro Leu His Leu Cys Gln Pro Leu Arg Met Ser Arg Thr Pro Pro

20 25 30

249

Arg Arg Val Cys Ala Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg

35 40 45

Trp Asn Pro Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln

50 55 60

Lys Pro Ser Gln Lys Asn His Leu Tyr Gly Gly Lys Lys Arg Ser Lys

65 70 75 80

Phe Ser Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val

85 90 95

Lys Val Leu Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly

100 105 110

Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys Ser Arg Arg Lys

115 120 125

Trp Gln Ile Arg His Val Leu Lys Asn Leu Trp Leu Pro Met Arg Ser

130 135 140

Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln Trp Gly Glu Ser Pro Trp

145 150 155 160

Gln Val Met Ala Ser Leu Thr Gly Arg Ala Thr Phe Leu Leu Ala Leu

165 170 175

Trp Glu Cys Ser Leu Pro Gln Ala Arg Leu Cys Leu Ile Val Ser Arg

180 185 190

Thr Leu Leu Leu Val Gln Ser Arg Asn Lys Lys Thr Lys Leu Trp Phe

195 200 205

Ser Leu Ile Asn Ile His His Arg Lys Asn Pro Leu Leu Pro Met Arg

210 215 220

Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys

225 230 235 240

Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly

245 250 255

Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Ile Arg Met Cys Ala

260 265 270

Leu Ser His Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser

275 280 285

Lys His Cys Met Thr Ala Cys Thr Arg Lys Arg Met Lys Thr Gly Ala

290 295 300

Ile Gln Glu Lys Gly Ala Ile Gln Glu Lys Gly Ala Met Thr Lys Arg

305 310 315 320

250

Ile Cys Val Val Asn Phe Glu Ala Cys Arg Glu Met Glu Ser Leu Ser

325 330 335

Ala Pro Glu Lys Ile Thr Leu Phe Glu Ala His Met Ala Arg Cys Thr

340 345 350

Ser Ile Asn Val Leu Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met

355 360 365

Lys Glu Lys Arg Lys Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile

370 375 380

Met Gln Arg Leu Phe Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe

385 390 395 400

His Leu Gln Asn Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr

405 410 415

Ile Asn Ile Leu Thr Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly

420 425 430

Ile Gln Val Met Leu Cys Ala Ser Pro Gln Ile Met Gln Arg Thr Ser

435 440 445

Ala Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn Leu Asp Asn Leu

450 455 460

Gly Val Pro Trp Pro Leu Leu Pro Gly Ile Pro Ser Leu Asn Ala Gln

465 470 475 480

Trp Met Phe Leu Arg Ile Ala Ser Pro Gln Ile Met Gln Arg Met Ser

485 490 495

Ala Val Asn Phe Glu Thr Ile Arg Ala Arg Asn Arg Asn Met Lys Lys

500 505 510

Thr Met Ser His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu

515 520 525

Arg Leu Phe His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Leu

530 535 540

Leu Leu Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly

545 550 555 560

Glu Cys Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu

565 570 575

Lys Gln Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Gly His Arg

580 585 590

Glu Gly Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro

595 600 605

251

Pro Tyr Pro Ile Ser Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile

610 615 620

Asn Ser Asn Ser Leu Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu

625 630 635 640

Cys His His Ala Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg

645 650 655

Leu Phe Ser Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala

660 665 670

Leu Cys Lys Phe Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys

675 680 685

Ser Val Tyr Ala Asn Met Cys Trp Pro Lys Phe Phe Leu Thr Phe Ser

690 695 700

Gln Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg

705 710 715 720

Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser

725 730 735

Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg

740 745 750

Val Leu Trp Met Pro Thr Ser Thr Val Leu Gln Ala His Pro Gln Val

755 760 765

Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu

770 775 780

Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu Thr Arg Glu Lys Ile Ile

785 790 795 800

Asn Pro Thr Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Ile

805 810 815

Tyr Pro Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Phe Leu Pro Ser

820 825 830

Arg Phe Pro Trp Gly Pro Pro Pro Leu Asn Ser Val Ile Val His Lys

835 840 845

Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu Arg

850 855 860

Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu Lys

865 870 875 880

Leu Leu Asn Met Asp Leu Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr

885 890 895

Ser Pro Leu Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn Cys Val

252

900 905 910

Val His Val Leu Met Phe Gly Ala Leu Arg Ala Thr Ala Pro Ala Thr

915 920 925

Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg Ala Ala Thr Thr Ser

930 935 940

Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr Ser Ser Ser Ser Ser Trp

945 950 955 960

Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu

965 970 975

Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr

980 985 990

Arg Ser Arg Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg Ser Ser

995 1000 1005

Leu Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr Ser

1010 1015 1020

Ser Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg Arg Gln Ile

1025 1030 1035

Asn Arg Arg Lys Leu Gln Arg Lys Lys His Trp Asn Ser Ala Tyr

1040 1045 1050

Leu Gly Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr His Arg Trp

1055 1060 1065

Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp Leu Thr Ser Thr

1070 1075 1080

Thr Ser Arg Ser Ser Ala Leu Met Trp Thr Glu Leu Ser Leu Glu

1085 1090 1095

Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Arg Thr Leu

1100 1105 1110

Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln Cys Gln Glu

1115 1120 1125

Lys Lys Ser Arg Lys Leu Met Ser Ser Cys Thr His Pro Cys Thr

1130 1135 1140

Met Leu Leu Pro Gln Gly Cys His Pro Ala Trp Leu Ser Pro Ile

1145 1150 1155

Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His Val Leu Ala

1160 1165 1170

Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro Gln Met Gln

1175 1180 1185

253

Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys Pro Gly

1190 1195 1200

Thr Ser Leu Gln Cys Tyr Leu Asn Arg Arg Arg Asn Ser Arg Lys

1205 1210 1215

Gln Ser Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp Lys

1220 1225 1230

His Lys Tyr Phe Leu Phe Gly Gln Lys Lys Gly His Met Ile Phe

1235 1240 1245

Ser Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Asn Ser Arg

1250 1255 1260

Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr Gly Lys

1265 1270 1275

Gly Leu Thr His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn Lys

1280 1285 1290

Arg Asn Lys Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile

1295 1300 1305

Lys Arg Lys Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro

1310 1315 1320

Glu Ser Asn Leu Ile Leu Ile Gln His Gly Gly Ile Phe Thr Phe

1325 1330 1335

Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu

1340 1345 1350

Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr

1355 1360 1365

Ile Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe His Val Pro

1370 1375 1380

Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr Thr Glu

1385 1390 1395

Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys

1400 1405 1410

Tyr Gln Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys

1415 1420 1425

Gly Thr Pro Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg Ala

1430 1435 1440

Phe Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr

1445 1450 1455

254

Gly Cys Trp Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr Arg

1460 1465 1470

Arg Lys Lys Arg Glu Asp His Cys Gly Leu Arg His Pro Ser Trp

1475 1480 1485

Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro Arg Ser

1490 1495 1500

<210> 1090

<211> 1506

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 3A polypeptide

<400> 1090

Gly Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr

1 5 10 15

Thr Pro Ser Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln

20 25 30

Ser Lys Lys Thr Met Thr Ala Ala Gly Leu Thr Pro Met Ile Arg Lys

35 40 45

Ile Gln Lys Arg Arg Arg Thr Ala Asn Arg Cys Leu Arg Val Arg Cys

50 55 60

Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Gln Asn Thr Arg Ser

65 70 75 80

Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln Arg Arg

85 90 95

Gly Lys Leu Ser Ser Leu Phe Phe Ser His Cys Leu Cys Arg Asn Ser

100 105 110

Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu

115 120 125

Arg Val Pro Ile Pro Pro His Leu Arg His Pro Leu Leu Leu Met Asp

130 135 140

Leu Asp Gly Met Leu Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr

145 150 155 160

Pro Arg Asp Leu Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg

165 170 175

Pro Ile Gln Val Gln Thr Gly Gly Arg Leu Thr Pro Pro Pro Leu Ile

180 185 190

Leu Trp Pro Gln Ala Ala Pro Val Asp Pro Pro Gly Ser Val Trp Ile

195 200 205

255

Leu Pro Cys Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys Arg Leu

210 215 220

Asp Phe His Pro Arg Ser Ser Ser Gly Val Ser Val Ile Thr Met Lys

225 230 235 240

Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn Leu Val Val Trp

245 250 255

Ile Leu Asn Leu Lys Met Met Asn Ile Glu Ile Thr Pro Gln Arg Ser

260 265 270

Lys Ala Arg Lys Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro Ser

275 280 285

Glu Lys Lys Arg Lys Leu Pro Leu Pro Leu Thr Phe Ser Thr Leu Leu

290 295 300

Trp Asp Ser Arg His Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser

305 310 315 320

Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met Arg

325 330 335

Leu Tyr Gln Phe Leu Ile His Leu Arg Lys Val Asp Thr His His Leu

340 345 350

Gln Val Val Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly Gln Arg

355 360 365

Met Arg Arg Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro

370 375 380

Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser His Phe Pro Pro His Pro

385 390 395 400

Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln Leu Arg Ser Ile Pro His

405 410 415

Val Pro Lys Ala Thr Ala Ala His Ser Val Thr Val Lys Leu Gln Lys

420 425 430

Gly Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln

435 440 445

Gly His Leu Leu Met Ile Leu Leu His Asn Thr Tyr Met Glu Met His

450 455 460

Tyr Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu Leu Cys

465 470 475 480

Arg Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His Pro Gln

485 490 495

256

Gly Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser Val Leu

500 505 510

Leu Leu Gly Val Pro Gln Thr Ala Ala Arg Gln Pro Ser Arg Pro Thr

515 520 525

Gly Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met

530 535 540

Ser Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met

545 550 555 560

Pro Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser

565 570 575

Pro Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile

580 585 590

Ser Arg Ala Thr Trp Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser

595 600 605

Phe Ser Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Ser Arg

610 615 620

Arg Asn Arg Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu

625 630 635 640

Gln Ala Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser Pro

645 650 655

Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala Ile Gly

660 665 670

Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala

675 680 685

Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr Met Val Met Lys His

690 695 700

Leu Asn Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr

705 710 715 720

Met Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile Pro

725 730 735

Leu Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His Pro

740 745 750

Pro Pro Pro Cys Leu Leu Leu Leu His His Arg His His Leu Ala Phe

755 760 765

Leu Glu Met Ala Cys Ser Ser Gln His Thr His Ser Ile Lys Ile Trp

770 775 780

257

Ile Ser Phe Ser His Glu Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp

785 790 795 800

Asn Thr Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile Ala

805 810 815

Gly Ala His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys

820 825 830

Met Asn Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe Ser

835 840 845

Pro Leu Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly Lys

850 855 860

Arg Cys Asn Pro Cys Cys Arg Leu Thr Trp Met Ser Met Leu Gln Lys

865 870 875 880

Gln Cys Cys Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile

885 890 895

Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu

900 905 910

Gly Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys

915 920 925

Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Arg Glu

930 935 940

Trp Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu

945 950 955 960

Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val Leu Ala

965 970 975

Gly Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln Ser Glu

980 985 990

Thr Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg Gly Pro Val Tyr

995 1000 1005

Tyr Val Cys Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Phe

1010 1015 1020

Leu Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys Thr Leu Gly

1025 1030 1035

Arg Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys Leu Ile

1040 1045 1050

Pro Val Pro Ile Asp Leu Pro Arg Met Leu Pro Ile Ser Ser Pro

1055 1060 1065

Leu His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr Phe Pro Ala

258

1070 1075 1080

Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp Gly Trp Arg

1085 1090 1095

Thr Phe Arg Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu

1100 1105 1110

Pro Ala Asn Gln Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val

1115 1120 1125

Leu Arg Thr Glu Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg

1130 1135 1140

Ser Phe Leu Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln Met

1145 1150 1155

Thr Asn Val Gly Cys Ala Phe Ser Gly Arg Ile Leu His Gln Pro

1160 1165 1170

Glu Met Ala Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr

1175 1180 1185

Asn Ser Trp Trp Thr Cys Thr Asn Leu Ser Val Pro Met Met Leu

1190 1195 1200

Thr Ile Leu Ile Trp Lys Arg Val Phe Ile Leu Leu Leu Ser Asp

1205 1210 1215

Lys Lys Leu Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg

1220 1225 1230

Asn Val Ser Arg Ile Ser His Gln Met Lys Cys Leu Thr Ala Val

1235 1240 1245

Asp Cys Leu Gln Ala Phe Trp Asp Met Asn Met Glu His Arg Leu

1250 1255 1260

Thr Met Asp Ser Ala Leu Ser Leu Thr Lys Arg Glu Gly Pro Glu

1265 1270 1275

Thr Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu Thr Met Ser Leu

1280 1285 1290

Ser Asn Ile Leu Met Lys Phe Arg Leu Arg Leu Arg Ile Pro Ser

1295 1300 1305

Leu Arg Lys Tyr Arg Gln His Leu Lys Lys Thr Ser Ala Lys Gly

1310 1315 1320

Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly Arg Gly Pro Pro

1325 1330 1335

Leu His Thr Arg Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys

1340 1345 1350

259

Lys Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu Arg Arg Asn

1355 1360 1365

Leu Gln Arg Asn Leu Lys Val Leu Trp Leu Lys Lys Ser Thr Lys

1370 1375 1380

Ser Arg Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Phe Glu Lys

1385 1390 1395

Lys Arg Pro Glu Trp Leu Ser Ser Arg Lys Lys Gly His Thr Lys

1400 1405 1410

Asn Arg Arg Lys Ala Lys Arg Lys Pro Gln Ile Val His Lys Lys

1415 1420 1425

Arg Gly Leu Phe Leu Lys Lys Lys Tyr Leu Val Gln Gly Ala Lys

1430 1435 1440

Lys Ile Thr Phe Leu Ser Asn Gly Met Glu Ala Cys Gly Thr His

1445 1450 1455

Pro Thr Pro Arg Leu Ser Asp Arg Val Phe Leu Val Lys Ile Thr

1460 1465 1470

Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg

1475 1480 1485

Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Leu Cys Phe Phe Tyr

1490 1495 1500

Leu Leu Tyr

1505

<210> 1091

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 4A polypeptide

<400> 1091

Ile Gln Lys Lys Lys Gln Lys Ser Met Ala Lys Lys Ser Gln Gln Pro

1 5 10 15

Leu Val Leu Lys Lys Asn Arg Lys Gln Glu Trp Gly Gly Gln Ala His

20 25 30

Arg Gln Arg Asn Asn Ala Lys Lys Met Pro Phe Gln Glu Glu Glu Lys Lys

35 40 45

Asn Gly Arg Ile Ser Ser Ser Leu Ile Leu Ser Phe Gln Arg Val Lys

50 55 60

Asp Ser Lys Lys Met Thr Gln Lys Met Cys Ser Ser Leu Ala Leu Pro

260

65 70 75 80

Thr Gly Leu Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu

85 90 95

Ile Ser Ser Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro

100 105 110

Ser Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp Thr

115 120 125

Trp Ser Ser Trp Leu Lys His Pro Arg Thr Ala Ser Ile Thr Leu Pro

130 135 140

Thr Thr Ser Leu Lys Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His

145 150 155 160

Pro Arg Leu Pro Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser

165 170 175

Leu Gly Thr Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met

180 185 190

Arg Ser Leu Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His His

195 200 205

Cys Arg Thr Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln

210 215 220

Gly Gln Trp Ser Gly Ala Val Val Gly Leu Arg Pro Val Ala His His

225 230 235 240

Leu Pro Arg Pro Ile Gln Gly Leu His Cys Pro Gln Ala Pro His Gln

245 250 255

Pro Ala Leu Ser Trp Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala

260 265 270

Arg Cys Cys Arg Trp Pro Arg Arg Ser Gln Lys Ser Gln His His Cys

275 280 285

Ala Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly Lys

290 295 300

Ala Gly Pro Gln Ala Gln Glu Lys Ala Arg Pro Ser Pro Cys Leu Ser

305 310 315 320

Trp Ser Ser Arg Ile Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr

325 330 335

Arg Arg Arg Trp Ser Ser Lys Cys Arg Gly Gly Pro Ser Ala Ser Asp

340 345 350

Arg Cys Cys Ser Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg

355 360 365

261

Cys Pro Val Gln Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg

370 375 380

Arg Trp Gly Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe

385 390 395 400

Gln Leu Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg

405 410 415

Lys Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val

420 425 430

Thr Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala Leu

435 440 445

Glu Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn

450 455 460

Leu Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Cys Gln

465 470 475 480

Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu Val Pro Pro Ser Pro

485 490 495

Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys Thr Gln Leu Ala Arg

500 505 510

Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile Leu Gly Pro Gln Arg

515 520 525

His Thr Pro Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe Phe

530 535 540

Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys Asn Glu

545 550 555 560

Gly Asn Asn His Trp Pro Arg Val Glu Met Pro Thr Gly Trp Leu Leu

565 570 575

Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln

580 585 590

His Leu Lys His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu

595 600 605

Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe Lys Thr

610 615 620

Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro

625 630 635 640

Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr Thr Gly

645 650 655

262

Val Tyr Gln His Val Trp Leu Ile Cys Phe Val Phe Phe Val Cys Val

660 665 670

Glu Thr Gly Phe Cys His Val Ala Gln Val Gly Leu Glu Arg Leu Val

675 680 685

Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro Lys Pro

690 695 700

Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu Arg Val

705 710 715 720

Gly Lys Gly Val Trp Ala Arg Leu Leu Lys Lys Lys Pro Arg Asp Ile

725 730 735

Cys Arg Ile Ser Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His

740 745 750

Arg Lys Thr Asp Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr

755 760 765

Thr Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro

770 775 780

Pro Arg Cys Arg Cys Arg Arg Glu Tyr Arg Val Thr Met Asn Ile Leu

785 790 795 800

Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu Thr Ala

805 810 815

Lys Lys Ser Pro Thr Ser Phe Cys Cys Asn Leu Asp Leu Gln His Gln

820 825 830

Arg Arg Arg Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser

835 840 845

Pro Lys Ala Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln

850 855 860

Asn Leu Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg

865 870 875 880

Lys Asp Glu Glu Asp His Gln Lys Arg His His His His Asn Gln Lys

885 890 895

Lys Met Ser Val Pro Leu Phe Phe His Gly Asp Leu Cys Arg Glu Ser

900 905 910

Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Gln Arg Pro

915 920 925

Pro Leu Gln Pro Val Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu

930 935 940

263

Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu

945 950 955 960

Cys Thr Ser Arg Ile Leu Asn Leu Gln Val Leu Lys Lys Ile Leu Arg

965 970 975

Ser Phe Met Lys Leu Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg

980 985 990

Thr Lys Leu Glu Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His

995 1000 1005

Ser Tyr Lys Ile Thr His Pro Phe Ala Ser Gln Pro Leu Gly Gln

1010 1015 1020

Glu Met Pro Asn Asn Thr Gln Ile Arg Leu Leu Lys Pro Asp Lys

1025 1030 1035

Lys Lys Lys Ser Thr Glu Lys Thr Lys Gly Gln Ala Phe Gly Ser

1040 1045 1050

Phe Ser Ala Asp Phe Ser Ala Pro Gln Gly Gln Ala Phe Gly Ser

1055 1060 1065

Leu Cys Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr

1070 1075 1080

Arg Leu Arg Ser Leu Asn His Leu Leu Ile Trp Leu Lys Asn Trp

1085 1090 1095

Lys Arg Phe Lys Leu Lys Lys Lys Tyr Gln Ala Pro Leu Arg Leu

1100 1105 1110

Trp Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser Ile Ile

1115 1120 1125

Cys Ile Lys Asn Met Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu

1130 1135 1140

Ser His Val Glu Lys Phe Glu Leu Lys Lys Asn Leu Leu Leu Pro

1145 1150 1155

Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Met Leu Asn Gln

1160 1165 1170

Lys Cys Leu Ala Pro Phe Pro Ala Cys Thr Ser Lys Ala Trp Thr

1175 1180 1185

Pro Arg Lys Leu Val Gly Arg Ala Val Arg Arg Lys Gly Ile Leu

1190 1195 1200

Ala Met Thr Leu Thr Leu Asn Gly Ile Glu Ala Leu Thr Ala Leu

1205 1210 1215

Phe Thr Cys Arg Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu

264

1220 1225 1230

Met Ser Ile Leu Glu Ala Ile Ile Lys Lys Met Lys Lys Arg Ile

1235 1240 1245

Met Val His Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met

1250 1255 1260

Leu Cys Leu Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser

1265 1270 1275

Asn Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys

1280 1285 1290

Gln Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro

1295 1300 1305

Asn Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp

1310 1315 1320

His Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser

1325 1330 1335

Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met

1340 1345 1350

Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp

1355 1360 1365

Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Ser Pro

1370 1375 1380

Leu Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu

1385 1390 1395

Trp Glu Ile Pro Ser Val Thr Leu Leu Ala Asn Leu His Leu Cys

1400 1405 1410

Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg Gln Met Glu

1415 1420 1425

Ser Leu Gly Met Lys Leu Gln Leu Lys Lys Lys Val Asn Ala Cys

1430 1435 1440

Ser Thr Tyr Thr Pro His Ile Cys Arg His Thr Cys Arg Val Asn

1445 1450 1455

Ala Val Glu Glu Leu His Ser Arg Arg Met Ser Val Arg Ile Val

1460 1465 1470

Lys Asn Trp Val Ser Cys Cys Tyr Val Arg Leu Ser Ala Val Gly

1475 1480 1485

Leu Ser Thr Trp Ser Ala Leu Asp Asn Lys Ile Ile Cys Lys Gly

1490 1495 1500

265

His Arg Ile Thr

1505

<210> 1092

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 1B polypeptide

<400> 1092

Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser

1 5 10 15

Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro

20 25 30

Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala Met Pro Ser Ser Trp

35 40 45

Ser Ser Ser Gly Arg Thr Gln Arg Lys Val Val Lys Asn Pro Ser Gln

50 55 60

Pro Val Pro Leu Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr

65 70 75 80

Arg Ser Cys Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu

85 90 95

Met Met Lys Lys Lys Asp Ser Thr Ile Phe Ile Val Pro Met Ser Leu

100 105 110

Ala Cys Ser Ile Thr Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val

115 120 125

Leu Asp Val Ser Ser Ile Ser His Trp Asn Lys Val Arg Gln Asn Phe

130 135 140

Lys Arg Asn Ala Asp Lys Lys Asn Val Arg Ser Gly Glu Lys Asn Asp

145 150 155 160

Leu Gln Leu Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr

165 170 175

Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr Thr Ser

180 185 190

Leu Gly Asp Cys Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His

195 200 205

Pro Ala Ser Gly Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg

210 215 220

Ala Cys Arg Ala Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Phe Asp

266

225 230 235 240

Lys Lys Gly Phe Val Ile Leu Ser Val Gly Ala Gly Thr Gly Gly Gly

245 250 255

Pro Ala Cys Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala

260 265 270

Glu Gly Ala Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg

275 280 285

Gln Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg

290 295 300

Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro Lys

305 310 315 320

Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg Ser

325 330 335

Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro Arg

340 345 350

Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg

355 360 365

Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro

370 375 380

Ser Cys His Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln

385 390 395 400

Ala Pro Thr Ala Phe Phe Leu Leu Asp Ile Ile Leu Arg Leu Pro Cys

405 410 415

Lys Val Thr Glu Ser Ser Asn Met Gly Gly Asn Arg Ser Ile Cys Leu

420 425 430

Thr Asn Ala Lys Lys Met Glu Asp Met Ile Trp Glu Ser Thr Pro Leu

435 440 445

Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro

450 455 460

Gly Leu Thr His Pro Ala His Gln Pro Leu Gly Ser Met Thr Leu Ala

465 470 475 480

Ile Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala

485 490 495

Ala Pro Ser Gly Gln Tyr Phe Lys Gly Lys Lys Arg Arg Lys Glu Ile

500 505 510

Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser Pro Ser Leu Ala Lys Lys

515 520 525

267

Thr Leu Thr Asn Ser Ser Phe Phe Tyr Thr Ser Asp Arg Glu Ala Lys

530 535 540

Lys Ser Met Lys Arg Ser Met Thr Leu Lys Ala Leu Val Lys Thr Trp

545 550 555 560

Thr Gln Phe Arg Asn Tyr Pro Arg Ala Ser Ser Pro Gly His Pro Leu

565 570 575

Gly Pro Asn Gly Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys

580 585 590

Ile Ser Val Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile

595 600 605

Thr Glu Cys Val Arg Thr Ala Leu Asn Arg Arg Ser Lys Ile Glu Ala

610 615 620

Ser Pro Ile Gly Ser Ile Ala Leu Arg Ser Met Pro Lys Ser Arg Asn

625 630 635 640

Gln Val Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu

645 650 655

Ala Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser

660 665 670

Arg Lys Gln Ile Pro Ser Gln Ser Met Leu Val Ala Ser Arg Ala Gly

675 680 685

Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly Met Ser Leu Gln Trp

690 695 700

Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys Pro Ser Arg Ser Pro

705 710 715 720

Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp His Arg Ser Gly Leu Arg

725 730 735

Ala Gln Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg Gln

740 745 750

Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp Ser

755 760 765

Lys Leu Arg Ser Pro Trp Ile Phe Val Ser Lys Lys Arg Leu Pro Lys

770 775 780

Leu Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile

785 790 795 800

Thr Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro

805 810 815

268

His Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser His

820 825 830

His Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly

835 840 845

Gly Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Arg Ser Leu Leu Tyr

850 855 860

Ser Arg Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp

865 870 875 880

Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe

885 890 895

Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser

900 905 910

Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Arg His Pro His Arg

915 920 925

Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro

930 935 940

Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala

945 950 955 960

Thr Gly Leu His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe

965 970 975

Leu Leu His Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser His Arg

980 985 990

Leu Arg Leu Arg Leu Pro Arg Thr Gln Arg Pro Pro Arg Ser Ile Leu

995 1000 1005

Ser Leu Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu

1010 1015 1020

His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Tyr

1025 1030 1035

Pro Arg Leu Lys Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys

1040 1045 1050

Leu Ala Gly Ser Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg

1055 1060 1065

Gln Glu Cys Gly Arg Arg Ala Ser Val Pro Cys Arg Pro Met Ile

1070 1075 1080

Gly Ser Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser

1085 1090 1095

269

Ala Met Gly Val Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser

1100 1105 1110

Pro Pro Ala Thr Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met

1115 1120 1125

Met Ser Trp Met Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys

1130 1135 1140

Ser Pro Ala Pro Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro

1145 1150 1155

Thr Ala Val Pro Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro

1160 1165 1170

Val Leu Ser Gly Cys His Leu Ala Val Arg Thr Ser Met Leu Thr

1175 1180 1185

Leu Leu Pro Met Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr

1190 1195 1200

Leu Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Ser Thr

1205 1210 1215

Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro

1220 1225 1230

Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys

1235 1240 1245

Leu Arg Leu Arg Asn Thr Ser Pro Ile Val Phe Phe Leu Ala Pro

1250 1255 1260

Tyr Phe Pro Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys

1265 1270 1275

Met Met Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg

1280 1285 1290

Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr

1295 1300 1305

Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg

1310 1315 1320

Gln Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr

1325 1330 1335

Ser Leu Ala Ile Trp Gly Asn Gln Gln Leu Lys Trp Thr Pro His

1340 1345 1350

Ile Leu Lys Ser Ala Ser Arg Arg Ala Arg Pro Thr Ser Cys Ser

1355 1360 1365

Thr Cys Arg Met Trp Trp Ile Ala Lys Lys Arg Ile Lys Leu Arg

270

1370 1375 1380

Arg Asn Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly

1385 1390 1395

Thr Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr Cys

1400 1405 1410

Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala

1415 1420 1425

Pro Val Gly Ala Gln Ala Pro Pro Glu Val Thr Leu Arg Ala Ser

1430 1435 1440

Cys Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp

1445 1450 1455

Gly Ala Gln Val Arg Gln Pro Ser Leu Pro Gly Pro Cys Ala Ser

1460 1465 1470

Leu Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro Ser Gln

1475 1480 1485

Val Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro Ala Ala

1490 1495 1500

Ala Cys Pro Pro

1505

<210> 1093

<211> 1501

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 2B polypeptide

<400> 1093

Arg Lys Met Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Met Ser

1 5 10 15

Arg Thr Pro Pro Arg Arg Val Cys Asn Pro Arg Arg Lys Thr Trp Lys

20 25 30

Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn His Leu Tyr

35 40 45

Asn Ser Arg Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr

50 55 60

Gly His Arg Glu Gly Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser

65 70 75 80

Phe Pro Pro Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe Tyr Ile

85 90 95

271

Ser Ser Thr Val Cys Phe Leu Ser Lys His Cys Met Thr Ala Cys Thr

100 105 110

Gln Lys Lys Gly His Met Ile Phe Ser Arg Glu Lys Ile Ile Asn Pro

115 120 125

Thr Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Glu Leu Ser

130 135 140

Leu Glu Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Gly Gly

145 150 155 160

Lys Lys Arg Ser Lys Phe Pro Gly Thr Ser Leu Gln Cys Tyr Leu Asn

165 170 175

Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys Ser Ala Ser Pro

180 185 190

Gln Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg

195 200 205

Met Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly

210 215 220

Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys Ile

225 230 235 240

Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val His Val Leu Met Phe

245 250 255

Trp Leu Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln

260 265 270

Trp Gly Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Pro Tyr

275 280 285

Pro Ile Ser Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser

290 295 300

Asn Ser Leu Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His

305 310 315 320

His Ala Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe

325 330 335

Ser Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys

340 345 350

Lys Phe Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro

355 360 365

Gln Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln

370 375 380

Ser Ala Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Ser Arg

272

385 390 395 400

Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Leu Ala

405 410 415

Asn Gly Leu Trp Phe Asn Leu Ile Phe Val Leu Phe Lys Ala Asp Gln

420 425 430

Ser Glu Ser Ser Leu Ser Ser Gly Arg Asn Lys Lys Thr Lys Leu Trp

435 440 445

Phe Ser Leu Ile Asn Ile His His Arg Lys Asn Pro Leu Leu Pro Met

450 455 460

Arg Ile Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Leu Leu

465 470 475 480

Leu Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu

485 490 495

Cys Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys

500 505 510

Gln Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Leu Gln Ala His

515 520 525

Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg

530 535 540

Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu Thr Ala Ser

545 550 555 560

Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Ile

565 570 575

Tyr Pro Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Arg Thr Leu Glu

580 585 590

Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln Cys Thr His Pro Cys

595 600 605

Thr Met Leu Leu Pro Gln Gly Trp Trp Cys Thr Arg Gly Gly Gly Lys

610 615 620

Arg Lys Lys Lys Met Cys Lys Val Ser Gly Gly Cys Thr Ala Cys Val

625 630 635 640

Glu Arg Cys Pro Trp Lys Gly Ile Cys Trp Lys Trp Pro Val Thr Val

645 650 655

Ala Ala Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu

660 665 670

Arg Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp

675 680 685

273

Ser Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr

690 695 700

Arg Val Leu Trp Met Pro Thr Ser Thr Val Arg Arg Arg Asn Ser Arg

705 710 715 720

Lys Gln Ser Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp Lys

725 730 735

His Lys Tyr Phe Leu Phe Gly Arg Ala Arg Asn Arg Asn Met Lys Lys

740 745 750

Thr Met Ser His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu

755 760 765

Arg Leu Phe His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Phe

770 775 780

Leu Pro Ser Arg Phe Pro Trp Gly Pro Pro Pro Leu Cys His Pro Ala

785 790 795 800

Trp Leu Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe

805 810 815

His Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro

820 825 830

Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys

835 840 845

Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln

850 855 860

Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala

865 870 875 880

Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe

885 890 895

Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg

900 905 910

Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg

915 920 925

Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly

930 935 940

Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe

945 950 955 960

Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr Arg Arg Lys

965 970 975

274

Lys Arg Glu Asp His Cys Gly Leu Leu Ile Leu Ile Gln His Gly Gly

980 985 990

Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys

995 1000 1005

Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu

1010 1015 1020

Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe

1025 1030 1035

His Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys

1040 1045 1050

Tyr Thr Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln

1055 1060 1065

Gln Gln Cys Tyr Gln Gln Glu Lys Lys Ser Arg Lys Leu Met Ser

1070 1075 1080

Ser Cys Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser

1085 1090 1095

Val Tyr Ala Asn Met Cys Trp Ala Phe Trp Lys Pro Pro Ser Gln

1100 1105 1110

Gly Ile Ser Ser Cys Leu Met Thr Gly Cys Trp Gly Ser Pro Pro

1115 1120 1125

Leu His Leu Cys Gln Pro Leu Arg Arg Tyr Lys Lys Arg Gly Val

1130 1135 1140

Ile Ala Trp Arg Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys

1145 1150 1155

Asn Leu Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln

1160 1165 1170

Val Met Leu Cys Gly Lys Gly Leu Thr His Pro Val Pro Leu Lys

1175 1180 1185

Arg Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val Asn

1190 1195 1200

Gln Lys Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu

1205 1210 1215

Ile Val Leu Asn Ser Pro Glu Ser Asn Pro Lys Phe Phe Leu Thr

1220 1225 1230

Phe Ser Gln Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr Gly

1235 1240 1245

275

Ala Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln

1250 1255 1260

Thr Pro His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser

1265 1270 1275

Pro Arg Ala Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp

1280 1285 1290

Pro Arg Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Trp

1295 1300 1305

Pro Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser

1310 1315 1320

Arg Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg Ser Ser Leu

1325 1330 1335

Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr Ser Ser

1340 1345 1350

Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr

1355 1360 1365

Pro Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg Asn Ser Val

1370 1375 1380

Ile Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser

1385 1390 1395

Tyr Asn Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile

1400 1405 1410

Glu Phe Ala Val Leu Lys Leu Leu Asn Met Asp Leu His Trp Asn

1415 1420 1425

Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr

1430 1435 1440

His Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp Leu

1445 1450 1455

Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr Arg Gln

1460 1465 1470

Ile Asn Arg Arg Lys Leu Gln Arg Lys Lys Arg His Pro Ser Trp

1475 1480 1485

Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro Arg Ser

1490 1495 1500

<210> 1094

<211> 1506

<212> PROTEIN

<213> Artificial sequence

276

<220>

<223> Assembled poly-FSP 3B polypeptide

<400> 1094

Gly Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr

1 5 10 15

Thr Pro Ser Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln

20 25 30

Ser Lys Lys Thr Met Thr Ala Ala Gly Leu Thr Pro Lys Asn Lys Lys

35 40 45

Arg Arg Lys Leu Arg Pro Lys Met Ile Arg Lys Ile Gln Lys Arg Arg

50 55 60

Arg Thr Ala Asn Arg Arg Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys

65 70 75 80

Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Arg Gly Pro Pro Leu His

85 90 95

Thr Arg Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu

100 105 110

Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly

115 120 125

Cys Ser Ile Pro His Val Pro Lys Ala Thr Ala Ala His Ser Val Thr

130 135 140

Val Lys Leu Gln Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser

145 150 155 160

Arg His Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg Leu Gln

165 170 175

Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn Asn

180 185 190

Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His His Cys Gln Tyr

195 200 205

Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp Lys Lys Met Met

210 215 220

Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp Ser Ser Pro Leu

225 230 235 240

Gln Leu Leu Leu His Pro His Gly Lys Arg Cys Asn Pro Cys Cys Arg

245 250 255

Leu Thr Trp Met Ser Gln Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn

260 265 270

277

Pro Ser Leu Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu Ser Ala Phe

275 280 285

Ser Gly Arg Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met

290 295 300

Pro Lys Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr Leu Thr Thr Leu

305 310 315 320

Ser Phe Val Leu Ile Glu Glu Ala Arg Gln Pro Ser Arg Pro Thr Gly

325 330 335

Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met Ser

340 345 350

Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met Pro

355 360 365

Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser Pro

370 375 380

Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile Ser

385 390 395 400

Arg Ala Thr Trp Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn Val

405 410 415

Ser Ser Val Leu Lys Pro Phe Leu Phe Thr Val Pro Ile Glu Asp Met

420 425 430

Arg Glu Cys Thr Leu Gly Arg Asn Arg Met Asn Val Ser Ser Val Leu

435 440 445

Lys Pro Cys Leu Ile Pro Val Pro Ile Ser Glu Lys Lys Arg Lys Leu

450 455 460

Pro Asn Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala Met Leu Gln Lys

465 470 475 480

Gln Cys Cys Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile

485 490 495

Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu

500 505 510

Gly Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys

515 520 525

Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Ser Arg

530 535 540

Lys Lys Gly His Thr Lys Asn Arg Arg Lys Ala Lys Arg Lys Pro Gln

545 550 555 560

Ile Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe His Leu Lys Lys Thr

278

565 570 575

Ser Ala Lys Trp Leu Lys Lys Ser Thr Lys Ser Arg Ser Asn Gly Met

580 585 590

Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu Ser Cys Leu Arg Val

595 600 605

Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Arg Lys Ser

610 615 620

Gly Val Met Lys Cys Trp Asp Leu Cys Pro Val His Lys Lys Arg Gly

625 630 635 640

Leu Phe Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly Lys Gly Lys

645 650 655

Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln Gly His

660 665 670

Leu Leu Met Ile Leu Leu His Asn Asp Arg Val Phe Leu Val Lys Ile

675 680 685

Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly Ser Arg Arg Asn

690 695 700

Arg Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala

705 710 715 720

Phe Leu Ile His Leu Arg Lys Val Asp Thr His His Leu Gln Val Val

725 730 735

Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg

740 745 750

Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala

755 760 765

Ala Gln Ala Pro Pro Lys Ser His Phe Pro Pro His Pro Ser Gly Pro

770 775 780

Ser Arg Thr Ser Ser Leu Gln Leu Arg Ser Leu Phe Phe Ser His Cys

785 790 795 800

Leu Cys Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys Leu Leu

805 810 815

Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser Arg Ile

820 825 830

Ser Thr Pro Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro Val Ser

835 840 845

Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp

850 855 860

279

Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met Thr Tyr Met Glu

865 870 875 880

Met His Tyr Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu

885 890 895

Leu Cys Arg Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His

900 905 910

Pro Gln Gly Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser

915 920 925

Val Leu Leu Leu Gly Val Pro Gln Thr Ala Arg Gly Pro Arg Arg Lys

930 935 940

Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln Ser Leu Gln Lys Ser

945 950 955 960

Gln Val Phe Gln Trp Val Leu Arg Thr Glu Val Ala Pro Arg Cys Pro

965 970 975

Arg Ser Ala Val Asp Ile Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala

980 985 990

Ser Cys Ala Ser Val Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser

995 1000 1005

Ala Pro Cys Gly Ala Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala

1010 1015 1020

Val Leu Gly Thr Met Val Met Lys Leu Cys Phe Phe Tyr Leu Leu

1025 1030 1035

Tyr Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln His Leu

1040 1045 1050

Asn Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr

1055 1060 1065

Met Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile

1070 1075 1080

Pro Leu Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu

1085 1090 1095

His Pro Pro Pro Pro Cys Leu Leu Leu Leu His His Arg His His

1100 1105 1110

Leu Ala Phe Leu Glu Met Ala Cys Ser Ser Gln His Thr His Ser

1115 1120 1125

Ile Lys Ile Trp Ile Ser Phe Ser His Glu Val Asp Pro Pro Gly

1130 1135 1140

280

Ser Val Trp Ile Leu Pro Cys Asp Leu Pro Arg Met Leu Pro Ile

1145 1150 1155

Ser Ser Pro Leu His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr

1160 1165 1170

Phe Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp

1175 1180 1185

Gly Trp Arg Thr Phe Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp

1190 1195 1200

Ser Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg

1205 1210 1215

Met Arg Leu Tyr Gln Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu

1220 1225 1230

Thr Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala

1235 1240 1245

Arg Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Cys Thr Asn Leu

1250 1255 1260

Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val Phe

1265 1270 1275

Ile Leu Leu Leu Ser Asp Lys Lys Gly Ala Lys Lys Ile Thr Phe

1280 1285 1290

Leu Pro Ile Pro His Leu Arg His Pro Leu Leu Leu Phe Glu

1295 1300 1305

Lys Lys Arg Pro Glu Trp Leu Ser Met Asp Leu Asp Gly Met Leu

1310 1315 1320

Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu

1325 1330 1335

Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln

1340 1345 1350

Val Gln Thr Gly Gly Arg Leu Arg Arg Asn Leu Gln Arg Asn Leu

1355 1360 1365

Lys Val Leu Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe

1370 1375 1380

Ser Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Arg Glu

1385 1390 1395

Trp Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys

1400 1405 1410

281

Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val

1415 1420 1425

Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val

1430 1435 1440

Gln Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg

1445 1450 1455

Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu Thr

1460 1465 1470

Lys Arg Glu Gly Pro Glu Thr Leu Lys Lys Lys Tyr Leu Val Gln

1475 1480 1485

His Gln Met Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe

1490 1495 1500

Trp Asp Met

1505

<210> 1095

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 4B polypeptide

<400> 1095

Ile Gln Lys Lys Lys Gln Lys Ser Asn Leu Asp Leu Gln His Gln Arg

1 5 10 15

Arg Arg Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro

20 25 30

Lys Ala Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn

35 40 45

Leu Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys

50 55 60

Asp Glu Glu Asp His Gln Lys Arg His His His His Asn Gln Lys Lys

65 70 75 80

Met Ser Val Met Ala Lys Lys Ser Gln Gln Pro Leu Gly Gln Ala Phe

85 90 95

Gly Ser Leu Cys Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val

100 105 110

Thr Arg Leu Arg Ser Leu Asn His Leu Leu Ile Asn Glu Gly Asn Asn

115 120 125

His Trp Pro Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr

130 135 140

282

Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys

145 150 155 160

His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln

165 170 175

Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu

180 185 190

Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys

195 200 205

Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln

210 215 220

His Val Trp Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly

225 230 235 240

Phe Cys His Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser

245 250 255

Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser

260 265 270

Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly

275 280 285

Val Trp Ala Arg Leu Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro

290 295 300

Thr Thr Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp

305 310 315 320

Pro Pro Arg Asn Ile Leu Asn Ser Leu Pro Ser Ser Met Glu Ile Ala

325 330 335

Val Leu Gln Glu Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His

340 345 350

Arg Lys Thr Asp Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn

355 360 365

Arg Asn Lys Ser Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln Ser

370 375 380

Pro Leu Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu

385 390 395 400

Trp Glu Ile Pro Ser Val Thr Leu Leu Ala Cys Arg Val Asn Ala Val

405 410 415

Glu Glu Leu His Ser Arg Arg Met Ser Val Arg Ile Val Lys Asn Trp

420 425 430

283

Val Ser Cys Cys Tyr Val Arg Leu Ser Ala Val Gly Leu Ser Thr Trp

435 440 445

Ser Ala Leu Asp Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Ser

450 455 460

Gln Lys Ser Gln His His Cys Ala Leu Arg Lys Ile Glu Trp Ala Thr

465 470 475 480

Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys Ala

485 490 495

Asn Ala Lys Lys Met Pro Phe Gln Arg Trp Ile Phe Ser Asn Gln Leu

500 505 510

Thr Val Gln Met Leu Cys Leu Pro Ile Glu Ala Leu Thr Ala Leu Phe

515 520 525

Thr Cys Arg Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met Ser

530 535 540

Ile Leu Glu Ala Pro Leu Phe Phe His Gly Asp Leu Cys Glu Glu Lys

545 550 555 560

Lys Asn Gly Arg Ile Ser Ser Gln Ala Pro Leu Arg Leu Trp Ser Trp

565 570 575

Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser Thr Ala Lys Lys Ser Pro

580 585 590

Thr Ser Phe Cys Cys Glu Trp Gly Gly Gln Ala His Arg Gln Arg Asn

595 600 605

Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu Lys Met Glu Ile Gln

610 615 620

Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu Cys Thr Tyr Thr Pro

625 630 635 640

His Ile Cys Arg His Thr Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg

645 650 655

Cys Cys Ser Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys

660 665 670

Pro Val Gln Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg

675 680 685

Trp Gly Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln

690 695 700

Leu Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys

705 710 715 720

Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val Thr

284

725 730 735

Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala Leu Glu

740 745 750

Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu

755 760 765

Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Thr Ser Arg

770 775 780

Ile Leu Asn Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys

785 790 795 800

Leu Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu Glu

805 810 815

Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Gln

820 825 830

Arg Pro Pro Leu Gln Pro Val Gln Val Val Leu Arg Leu Gly Asn Leu

835 840 845

Pro Pro Phe Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg

850 855 860

Thr Lys Met Leu Asn Gln Lys Cys Leu Ala Pro Phe Ile Thr His Pro

865 870 875 880

Phe Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile

885 890 895

Arg Leu Leu Lys Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser

900 905 910

Asn Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys Gln

915 920 925

Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro Asn Phe

930 935 940

Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp His Thr Gly

945 950 955 960

Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser Arg Ser Pro Leu

965 970 975

Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met Ala Phe Ala Ser Asp

980 985 990

Ser Ser Ile Arg Thr Thr Arg Val Ser Trp Cys Arg Arg Cys Gly Ala

995 1000 1005

Lys Ser Ala Ala Ala Ser Ser Leu Lys Lys Lys Pro Arg Asp Ile

1010 1015 1020

285

Cys Arg Ile Ser Asp Ser Lys Lys Met Thr Gln Lys Arg Glu Ser

1025 1030 1035

Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Asp Lys

1040 1045 1050

Lys Lys Lys Ser Thr Glu Lys Thr Lys Ile Ile Lys Lys Met Lys

1055 1060 1065

Lys Arg Ile Met Val His Met Cys Ser Ser Leu Ala Leu Pro Thr

1070 1075 1080

Gly Leu Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu

1085 1090 1095

Ile Ser Ser Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu

1100 1105 1110

Pro Ser Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met

1115 1120 1125

Trp Thr Trp Ser Ser Trp Leu Lys Trp Leu Lys Asn Trp Lys Arg

1130 1135 1140

Phe Lys Leu Lys Lys Lys Tyr Ile Leu Ala Met Thr Leu Thr Leu

1145 1150 1155

Asn Gly His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser

1160 1165 1170

Leu Lys Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg

1175 1180 1185

Leu Pro Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu

1190 1195 1200

Gly Thr Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met

1205 1210 1215

Arg Ser Leu Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His

1220 1225 1230

His Cys Arg Thr Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu

1235 1240 1245

Tyr Gln Gly Gln Trp Ser Gly Ala Val Val Gly Leu Arg Pro Val

1250 1255 1260

Ala His His Leu Pro Arg Pro Ile Gln Gly Leu His Cys Pro Gln

1265 1270 1275

Ala Pro His Gln Pro Ala Leu Ser Trp Gly Lys Trp Pro Val Gly

1280 1285 1290

286

Met Thr Gly Pro Ala Arg Cys Cys Arg Trp Pro Arg Arg Glu Leu

1295 1300 1305

Lys Lys Asn Leu Leu Leu Pro Cys Arg Cys Arg Arg Glu Tyr Arg

1310 1315 1320

Val Thr Met Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser

1325 1330 1335

Ala Pro Gln Val Leu Lys Lys Asn Arg Lys Gln Asn Lys Ile Ile

1340 1345 1350

Cys Lys Gly His Arg Ile Thr Ser Leu Ile Leu Ser Phe Gln Arg

1355 1360 1365

Val Lys Leu Lys Lys Lys Val Asn Ala Cys Ser Pro Ala Cys Thr

1370 1375 1380

Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg Ala Val Arg

1385 1390 1395

Arg Lys Gly Ile Ile Cys Ile Lys Asn Met Phe Leu Gln Arg Lys

1400 1405 1410

Gly Phe Leu Asn Leu Ser His Val Glu Lys Phe Val Pro Pro Ser

1415 1420 1425

Pro Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys Thr Gln Leu

1430 1435 1440

Ala Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile Leu Gly

1445 1450 1455

Pro Gln Arg His Thr Pro Cys Gln Lys Lys Val Pro Ser Ala Ser

1460 1465 1470

Pro Trp Gly Leu Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg

1475 1480 1485

Ile Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg

1490 1495 1500

Trp Ser Ser Lys

1505

<210> 1096

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 1A

<400> 1096

accgagatcc agctgaccat gaacgacagc aagcacaagc tggaaagccc cgctctgaaa 60

caggtgtcac cagcctctcc acctactcag cagcctcaga cacctcagga ttctagacag 120

gtgctggccc tggccaagaa aaccctgaca aatgcctcca gagccggaca gtttcctgtg 180

tggcggagaa caagcagagg catgagcctg cagtggaagt ccggcaatgc ctgcacacct 240

ctgagatgcc ctagcagaag ccctgccaca ttcctgacag ccctgaatgg ctggcacaga 300

tctggcctga gagcccagat gcctagctct tggtctagca gcggcagaac actgaagctg 360

gcccctctgc aagtgaccac cactctgaag ctgatcctgg tcatgctgga aatcgtgacc 420

gagcgctccc tgctgtactc cagaatgtgt ctggtgcaga tccccacaca cacctgggag 480

cccgattact tcgtgtccct gcctatgatg ttcagctggg ccacacagtc ttttcccgtg 540

ggcgccgtgt ttctgaccag acctgccatt ccttgctgca gctgtaccag ccacagcaac 600

ccttggagcc agagcaatca gcagctgaag tggacccctc acatcctgaa gtccgccagc 660

ttcgacaaga aaggcttcgt gatcctgagc cggcggagca agattgaggc ctctcctatc 720

ggctctatcg ccagctctcc tggacatcct ctgggaccta cactggccat cagattcatc 780

agcgcccctc agcctagcag ctctacagtg acagctgccc ctagcggcca gtactttatc 840

gccaagaagc ggatcaagct gcggcggaac aatatgggcg gcaacagatc catctgcctg 900

accagaaagc gcaagagaga gaagcggagc cagaagtcca ggcagaacca tctgcagctg 960

aaaagctgca gacggaagat cagcaactgg tccaagctga gaagcccctg gatcttcacc 1020

aagtgcgaga tctgcgtgaa gaacaccgcc atcctggcta tcaccctgat cacccctcag 1080

gaccctcatc tggaccctgt gtgtcaccct catagaaggc ctggatggaa gggccaccat 1140

agagtggtgt tctctcacca tcctagcggc agcacaaaga gccactggca gcaaagagaa 1200

1260

ccctctcagc ccgtgcctct gagaaacgat ggcatcacca acggcaacga ctccaccaga 1320

agctgctcca tcagcctgaa gaaaagcctg gaaaagatgc ccctgatgcg gagagccaga 1380

cctaccagct gtagcacctg tagaatgtgg tggaagggca agaaacggcg gaaagaaatc 1440

gtgcctatga gcctggcctg cagcatcacc gtgtcttttg tgggacagat cagcaccctg 1500

gtgctggatg tgtctagcat cagccattgg cggctgccct gtaaagtgac cgagagcagc 1560

tccagcttct tctacaccag cgacaggacc cctctgagca tcagcagacc aaccagcaga 1620

gctagacaga gctgttgtct gcccggactg acacaccctg ctcaccaacc tctgggcagc 1680

atgagcacta gaagagagat gccaagccag gccacacctt ctccacctgg accttggaga 1740

gccgctctgg gcaaagaagt gaagagatcc cagagcaagc tgcgcctgag aaacacaagc 1800

cctggggaga agaacgatct ccagctgttc gtgatgagcg acaccaccta caagatctac 1860

tggacagtga tcctgctgaa cccctgcggc aacctgcacc tgaaaacaac atcactgcag 1920

cggccacctc ggagcatcct gtctctgaat ggaatctggg aagtgatgcc ccggacactg 1980

gaaggctgca tcagcgtgaa ggatcacaga accagccaga accacagcaa gtgcatcacc 2040

gaatgtgtgc ggactgccct gaacgtgtcc aagaagagac tgcctaagct gatgaagaag 2100

2160

aagaaacccc tcactttcag ccccagcaga cggatgactc ggagaaccat gacaaccacc 2220

aaaatgatga tgatgcggcg gaccaagcgg agaaggcgca aaaggcggag aatgaccatg 2280

atgaccagac ggaccctgct gacaaagaaa acctgtctga cccctagaac cagcgccgcc 2340

acaaaagctc tgcctagaca gaccatcagc cagcggaggc ctattcagac cccacctgga 2400

acatctctgg ccatctgggg cagaacaaga ctgaccctga caggcaccag atccagcgga 2460

acaagagcct ctccttccgc cgtgtgcctg tgttgttgga cctgtccaag cagaagcttc 2520

ccagccgctt ctcctagtgg actgagagca cctgttggag cccaggctcc tcctgaagtg 2580

acactgagag cctcttgtgc ctctagcgcc tctaaagcct gtgctggact ttggccttgg 2640

ggagcccaag ttcgagttgg agctggaaca ggcggaggac ctgcttgtgt tcctgtggct 2700

gaagcttcta caggcgcatg gacagctgaa ggcgccgctg gaacaagatg tagagcctct 2760

agagcccctt gccgccaact tcctgctagc tcccctacca agtgtgaaat ttgtgtgaag 2820

cgcagacggc ggagaaagag aagcagccag tctagaagcc ccgtgcctaa aaagcgcgag 2880

aggatgatgc tgcctgtgcg ggccagaaca gctagatctg cacttcctca gggcagagca 2940

cctctggaag ggatgctgct tcctagagca ctgcctagcc tgctgagcag aatgcctgcc 3000

agagagagt cccggttttg gctggctctg caggcacttg gactcagacc tccacatctg 3060

cctagctgtc atcctctgcc tcaactgttg ggccctccac ctcctcatca ggccccaatg 3120

acactgaagg ccctggttaa gacctggaca cagttccgca actaccccag agccgacaag 3180

aagaatgtcc ggtctaccgc cttcttcctg ctggacatca tcctgggcga ttgcccaaga 3240

acaatggccg gaccttctgc ctctctgcat cctgcatctg gcagagcctc cagaaagacc 3300

tggcagagaa ggctgagagc ttgtagagca ggcggacctg ctggatggggc tagacaggcc 3360

3420

ctgtccacaa tgatgatcat tagcaccacc agacaagagt gcggcaggcg ggcttctgtg 3480

ccttgtagac ctatgatcgg aagcgccaga ccaggacctt ggcggacatc tgctatgcct 3540

agtgccatgg gagtcgccct gcctacctct tgtgaaagcg gaagatctcc tcctgccacc 3600

ggcggaagaa tgccaggatc tcagtctatc atgatgagct ggatgcctcc actggctcca 3660

tgtgccgctt gtccatgttc tcccgctcct acactgtgcc cagcacatcc tgctagagca 3720

ccaacagccg tgcctgcctt tacacctctg tctgctcatc ctgtgccagt gctgtctgga 3780

3840

atgaagcggt ccaacgctaa gaaaatggaa gatatgatct gggagagccg gctgccactg 3900

aggccacaaa gaggacctag acctctgcac agatggctgc agcagacaac cggctgtagc 3960

cagaggcggga tcgtgttctt tctggcccca tacttccctc ggcaccctca cagatctcca 4020

ccaacatctt gccccacctct gctggaagct ttcccactgc caagagcttc tctgaggggcc 4080

acagtgccaa gacatagctc tgttctcgcc accggtctgc acctccacag acagatttct 4140

ctgcccctga gaggattcct cctgcaccaa ccactgccag gacagcatct gtggctgtct 4200

cacagactga ggctgagact gccaggaca cagccatctt tgcctggacc atgtgcaagc 4260

ctgctgtcca cactgtctca gccaccacct caggctccat ctcaagtgtg gactgccgct 4320

acactgagat gtcctgctgt tcctgcagct gcctgtcctc ctgctctgag atccatgcct 4380

aagagccgga accaggtgct gttcagacgg aatctgaccc caagccagct gagaacactg 4440

gccccacctt atatgctgct gccccagtct ctgtcccaga gcctgagtag aaagcagatc 4500

cctagccagt ccatgctcgt g 4521

<210> 1097

<211> 4503

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 2A

<400> 1097

agaaagatgc agagcctgaa tcaccccagc agactgacca atggctctcc tccactgcac 60

ctgtgccagc ctctgagaat gagcagaacc cctcctagaa gagtgtgcgc cgctcctcca 120

catagactgc tgggcccttc cagatggaac cctcggagaa agacctggaa gatgcggaag 180

aagtacgccc agaagcctag ccagaagaac cacctgtacg gcggcaagaa gcgctctaag 240

ttcagcagaa gccacctgga cttcagcccc tactggatga agaaagtgaa ggtgctgttc 300

aaggccgacc agagcgagtc tagcctgagc agcggaagat gcagcctgat ggaacagatc 360

cctcacctga agtctcggcg gaagtggcag atcagacacg tgctgaagaa cctgtggctg 420

cccatgagaa gcctgggaga accttggaga gccggctttc agtggggaga gtctccttgg 480

caagtgatgg cctctctgac cggcagagcc acatttctgc tggccctttg ggaatgtagc 540

ctgcctcagg caagactgtg cctgatcgtg tctagaaccc tgctgctggt gcagagccgg 600

aacaagaaaa caaagctgtg gttcagcctg atcaacatcc accaccggaa gaacccactg 660

ctgcctatga gatggtggtg taccagaggc ggcggaaagc ggaaaaagaa aatgtgcaag 720

gtgtccggcg gctgcaccgc ctgtgttgag agatgtcctt ggaagggcat ctgctggaag 780

tggcctgtga cagtggccgc cattagaatg tgtgccctgt ctcaccctag cttctacatc 840

agcagcaccg tgtgctttct gagcaagcac tgcatgaccg cctgcaccag aaagagaatg 900

aagaccggcg ccatccaaga gaagggcgct attcaagaga aaggggccat gaccaagcgg 960

atctgcgtgg tcaatttcga ggcctgcaga gagatggaaa gcctgagcgc ccctgagaag 1020

atcaccctgt ttgaagccca catggcccgg tgcacctcca tcaatgtgct gtgtgtgcgg 1080

gccagcctga ttgagaagct gatgaaagaa aagcgcaaga tgaagcggaa ccaggtggcc 1140

tctccacaga tcatgcagcg gctgttcatc aagggctacc agaacaacca cttcaccttc 1200

catctgcaaa acttcgcctt cttcagctcc ttcggcaaca acaagtacat caatatcctg 1260

accatcctgt tccacgtgcg gaacatcgtg tacggcatcc aagtgatgct gtgcgctagc 1320

cctcagatta tgcagagaac cagcgccgac cggttccgga tgaagagaag aatgcagaat 1380

ctggacaacc tgggcgtgcc atggcctctg ttgcctggaa tcccatctct gaacgcccag 1440

tggatgttcc tgagaatcgc atccccgcaa atcatgcaga ggatgagcgc cgtgaacttc 1500

gagacaatcc gggccagaaa cgggaacatg aagaaaacca tgagccacct gatcgcccat 1560

1620

ccgggaaaac tgctgctcgc ccatatgtac ggcttcagca cctacggcac ccagaaaggc 1680

gagtgcgttt gtctgcagtg tctgagcggc agcagcctgt tcattctgaa gcagcccctg 1740

gacctgaaga tcagcaagac cttcatgggc cacagagagg gcagcgagtc tgataatctg 1800

ggcctgctgt atagcttccc tccaccttat cctatcagcg agggcctgac catgaccacc 1860

ggcagaaaca tcaacagcaa ctccctgatg accaactgcc tgttcagccc catcttcctg 1920

tgccaccacg ccatcatgca cagaaagatg acaagccctc acttcaggct gttcagcagc 1980

aagatcccac atcctcaggt gccatctgtg gtggccctgt gcaagttccg ggccttcatc 2040

cacacaccta gaagccctgg ctgcagcgtg tacgccaata tgtgctggcc caagttcttc 2100

ctgaccttca gccagcagtg gtccagcgtg acatctctgg aatggacagc ccaagagaga 2160

ggctgcagca gctggctgat gaagcagaca tggatgaagt cttggagcct gcgggacccc 2220

agctacagat ccatcctgga atacgtgtcc accagagtgc tgtggatgcc tacctctacc 2280

gtgctgcagg ctcatccaca agttccagct ctggctcctc aagcctggtg gcctgctaga 2340

agaggacctc tgacatctgc cccatccgct cagcagtccc tgaccagaga gaagattatc 2400

aaccccacca tcagctgccc ctttcagagc cccaccaaga gactgatcta ccctcctagc 2460

acctgttctc tgccctgcag aggcttcctg cctagcagat ttccttgggg cccacctcct 2520

ctgaacagcg tgatcgtgca caagatcgtg ctggtgttcc tgaagtccct gtccagctac 2580

aactgcgagc ggagaatcag cggcatccag aagagcca tcgagttcgc cgtgctgaaa 2640

ctgctgaata tggacctgcc tctcgagaac acacagatcc ccagaacaag cccactgtgg 2700

aagatcacaa tggtgctgac ataccccaac tgcgtggtgc atgtgctgat gtttggcgcc 2760

2820

gccacaacaa gcagcatgag cagcagccct agagccgagc ctacatcttc tagctcttgg 2880

gccacctgtt ggccgatg ctgctccagc tcttgttact ggtcactgtc tagctggcct 2940

ccagctggcg ccgctgaagc tacaaatacc agaaccagaa gccgcgagag ccagaggggt 3000

3060

cggaccagct ccagatacaa gaaaagaggc gtgatcgcct ggcggagaca gatcaataga 3120

3180

acctgtatcc tgtatcaccg gtggatcgtg acctccatgt gtcagtccca gagatggctg 3240

accagcacca caagtagatc tagcgccctg atgtggaccg agctgagcct ggaaccatgg 3300

attccctacc tgcatcagca gaaaacccag cggacactgg aatccgccgg actgtttcct 3360

tggctgcaca cccagtgcca agagaagaaa agcagaaagc

ccctgcacaa tgctgctgcc acagggatgt catcctgcct ggctgagccc tatctctgcc 3480

tgtccaagac tgctgccata tccttttcac gtgctggctc tggaagtgac ctttcctcct 3540

ggaaccgcca tgcctcagat gcagttgcag gctctggcta tgtgggagaa aggctgggat 3600

aagcctggca ccagcctgca gtgctacctg aacagaagaa gaaacagccg gaagcagagc 3660

caggtgcaac tggccattca ggtgctgctc gtggacaagc acaagtactt tctgttcggc 3720

caaaagaaag gccacatgat ctttagcctg gccaacggcc tgtggtttaa cctgattttc 3780

aactcccgga agaaactgct cgaggacatg aagcaaaatg gcttcaccgg caaaggcctg 3840

acaccccg tgcctctgaa gcggactctg cagaacaaaa gaaacaagaa tatcaaacgc 3900

gtcaaccaga agaagggcaa catcaagcgg aagtccatcc tgctgcacct gattgtgctg 3960

aatagccccg agagcaacct gatcctgatc cagcatggcg gcatcttcac ctttcggttc 4020

tgggtcgaga agctgtcctg gaacatctgc aagaacctga gcctgtacct ggacaagttc 4080

gtgcagagac tgctgtacgt gtacatcatc gagacaaacg tgttcgtgct gaacctgttt 4140

catgtgcccg tgaaccagat cttcatgatg gccttctacc tgaagtacac cgagaaggcc 4200

tgggtcatgg aactggaatg gctgatccaa cagcagtgct atcagggcag ctgcggcaga 4260

gcttgttctt gtggaagagg ctgtggcacc cctagaggcg attggaagag acatcctcct 4320

cggagagcct tctggaagcc tcctagccag ggcatctcta gctgtctgat gaccggctgt 4380

tggctcgaga agaaagctac ctggatgcgg cggacccgcc ggaagaagag ggaagatcat 4440

tgcggactga gacaccccag ctggccttgg acaagatgtc tgaggatgag gcctccaaga 4500

agc4503

<210> 1098

<211> 4518

<212> PROTEIN

<213> Artificial sequence

<220>

<223> polynucleotide 3A

<400> 1098

Gly Gly Ala Gly Cys Thr Gly Cys Thr Gly Cys Thr Gly Cys Cys Thr

1 5 10 15

Cys Thr Thr Gly Thr Cys Ala Gly Thr Cys Thr Cys Cys Thr Cys Cys

20 25 30

Thr Thr Cys Thr Cys Cys Thr Cys Gly Gly Gly Ala Ala Ala Cys Cys

35 40 45

Ala Cys Ala Cys Cys Thr Ala Gly Cys Thr Cys Cys Gly Ala Cys Gly

50 55 60

Cys Cys Ala Cys Cys Ala Gly Ala Cys Gly Gly Ala Cys Ala Ala Gly

65 70 75 80

Ala Cys Cys Thr Ala Thr Thr Gly Cys Cys Gly Cys Thr Cys Ala Gly

85 90 95

Ala Gly Cys Ala Ala Gly Ala Ala Ala Ala Cys Cys Ala Thr Gly Ala

100 105 110

Cys Ala Gly Cys Cys Gly Cys Cys Gly Gly Ala Cys Thr Gly Ala Cys

115 120 125

Cys Cys Cys Thr Ala Thr Gly Ala Thr Cys Cys Gly Gly Ala Ala Gly

130 135 140

Ala Thr Cys Cys Ala Gly Ala Ala Gly Cys Gly Gly Cys Gly Gly Ala

145 150 155 160

Gly Ala Ala Cys Cys Gly Cys Cys Ala Ala Cys Cys Gly Gly Thr Gly

165 170 175

292

Thr Cys Thr Gly Ala Gly Ala Gly Thr Thr Thr Ala Gly Ala Thr Gly Thr

180 185 190

Gly Gly Cys Gly Gly Cys Thr Gly Gly Cys Thr Gly Thr Gly Thr Gly

195 200 205

Gly Cys Thr Gly Gly Ala Cys Ala Cys Cys Thr Ala Gly Ala Gly Gly

210 215 220

Ala Cys Ala Gly Ala Ala Cys Ala Cys Cys Ala Gly Ala Ala Gly Cys

225 230 235 240

Ala Cys Cys Ala Ala Cys Cys Thr Gly Gly Ala Ala Ala Ala Gly Ala

245 250 255

Ala Cys Cys Cys Cys Ala Gly Cys Cys Thr Gly Ala Ala Gly Ala Ala

260 265 270

Gly Cys Ala Gly Ala Gly Cys Cys Ala Gly Cys Gly Gly Ala Gly Ala

275 280 285

Gly Gly Ala Ala Ala Gly Cys Thr Gly Ala Gly Cys Ala Gly Cys Cys

290 295 300

Thr Gly Thr Thr Cys Thr Thr Cys Ala Gly Cys Cys Ala Cys Thr Gly

305 310 315 320

Cys Cys Thr Gly Thr Gly Cys Cys Gly Gly Ala Ala Cys Ala Gly Cys

325 330 335

Ala Gly Ala Ala Gly Gly Gly Cys Cys Ala Cys Ala Cys Cys Thr Ala

340 345 350

Cys Ala Thr Cys Thr Ala Ala Gly Gly Gly Cys Ala Gly Cys Cys Thr

355 360 365

Gly Cys Gly Gly Cys Gly Gly Ala Ala Gly Thr Ala Cys Cys Thr Gly

370 375 380

Ala Gly Ala Gly Thr Gly Cys Cys Thr Ala Thr Thr Cys Cys Thr Cys

385 390 395 400

Cys Ala Cys Ala Cys Cys Thr Gly Ala Gly Ala Cys Ala Cys Cys Cys

405 410 415

Thr Cys Thr Gly Cys Thr Gly Cys Thr Gly Ala Thr Gly Gly Ala Cys

420 425 430

Cys Thr Gly Gly Ala Cys Gly Gly Ala Ala Thr Gly Cys Thr Gly Ala

435 440 445

Cys Cys Gly Thr Gly Ala Thr Cys Gly Gly Ala Cys Ala Gly Gly Gly

450 455 460

293

Ala Cys Thr Cys Gly Thr Gly Gly Gly Cys Ala Thr Gly Ala Cys Cys

465 470 475 480

Cys Cys Thr Ala Gly Ala Gly Ala Thr Cys Thr Gly Gly Gly Ala Ala

485 490 495

Cys Ala Gly Thr Gly Gly Cys Thr Gly Gly Cys Gly Cys Cys Ala Cys

500 505 510

Cys Gly Cys Thr Cys Ala Gly Ala Thr Thr Cys Ala Gly Ala Gly Gly

515 520 525

Cys Cys Thr Ala Thr Thr Cys Ala Gly Gly Thr Gly Cys Ala Gly Ala

530 535 540

Cys Cys Gly Gly Cys Gly Gly Cys Ala Gly Ala Cys Thr Thr Ala Cys

545 550 555 560

Ala Cys Cys Thr Cys Cys Thr Cys Cys Thr Cys Thr Gly Ala Thr Thr

565 570 575

Cys Thr Gly Thr Gly Gly Cys Cys Thr Cys Ala Gly Gly Cys Cys Gly

580 585 590

Cys Thr Cys Cys Thr Gly Thr Thr Gly Ala Thr Cys Cys Thr Cys Cys

595 600 605

Thr Gly Gly Cys Thr Cys Thr Gly Thr Gly Thr Gly Gly Ala Thr Thr

610 615 620

Cys Thr Gly Cys Cys Cys Thr Gly Cys Ala Ala Gly Ala Ala Cys Ala

625 630 635 640

Ala Gly Ala Ala Gly Cys Gly Gly Ala Gly Ala Ala Ala Gly Cys Thr

645 650 655

Gly Cys Gly Gly Cys Cys Cys Ala Ala Gly Cys Gly Gly Cys Thr Gly

660 665 670

Gly Ala Cys Thr Thr Thr Cys Ala Cys Cys Cys Thr Ala Gly Ala Thr

675 680 685

Cys Thr Thr Cys Thr Ala Gly Cys Gly Gly Cys Gly Thr Gly Thr Cys

690 695 700

Cys Gly Thr Gly Ala Thr Cys Ala Cys Cys Ala Thr Gly Ala Ala Gly

705 710 715 720

Cys Thr Gly Thr Gly Gly Gly Ala Gly Ala Ala Gly Cys Ala Cys Thr

725 730 735

Thr Cys Ala Ala Gly Gly Gly Cys Cys Thr Gly Ala Ala Cys Thr Gly

740 745 750

Gly Ala Ala Cys Cys Thr Gly Gly Thr Cys Gly Thr Cys Thr Gly Gly

294

755 760 765

Ala Thr Thr Cys Thr Gly Ala Ala Cys Cys Thr Gly Ala Ala Gly Ala

770 775 780

Thr Gly Ala Thr Gly Ala Ala Cys Ala Thr Cys Gly Ala Gly Ala Thr

785 790 795 800

Cys Ala Cys Cys Cys Cys Thr Cys Ala Gly Cys Gly Gly Ala Gly Cys

805 810 815

Ala Ala Gly Gly Cys Cys Ala Gly Ala Ala Ala Gly Thr Cys Cys Gly

820 825 830

Gly Cys Gly Thr Gly Ala Thr Gly Ala Ala Gly Thr Gly Cys Thr Gly

835 840 845

Gly Gly Ala Thr Cys Thr Gly Thr Gly Cys Cys Cys Cys Ala Gly Cys

850 855 860

Gly Ala Gly Ala Ala Gly Ala Ala Gly Ala Gly Gly Ala Ala Gly Cys

865 870 875 880

Thr Gly Cys Cys Thr Cys Thr Gly Cys Cys Thr Cys Thr Gly Ala Cys

885 890 895

Cys Thr Thr Cys Ala Gly Cys Ala Cys Cys Cys Thr Gly Cys Thr Gly

900 905 910

Thr Gly Gly Gly Ala Cys Thr Cys Thr Ala Gly Ala Cys Ala Thr Ala

915 920 925

Gly Ala Gly Gly Cys Gly Cys Cys Ala Gly Cys Thr Cys Cys Ala Gly

930 935 940

Cys Ala Cys Cys Ala Gly Cys Ala Gly Ala Gly Ala Cys Thr Cys Cys

945 950 955 960

Ala Gly Ala Ala Gly Cys Thr Thr Thr Thr Thr Cys Ala Cys Cys Ala

965 970 975

Gly Cys Gly Cys Thr Cys Ala Cys Cys Cys Cys Ala Gly Cys Ala Gly

980 985 990

Ala Cys Cys Cys Thr Gly Cys Ala Gly Ala Ala Thr Gly Ala Gly Ala

995 1000 1005

Cys Thr Gly Thr Ala Cys Cys Ala Gly Thr Thr Cys Cys Thr Gly

1010 1015 1020

Ala Thr Cys Cys Ala Cys Cys Thr Gly Ala Gly Ala Ala Ala Gly

1025 1030 1035

Gly Thr Gly Gly Ala Cys Ala Cys Cys Cys Ala Cys Cys Ala Thr

1040 1045 1050

295

Cys Thr Gly Cys Ala Gly Gly Thr Cys Gly Thr Gly Ala Cys Cys

1055 1060 1065

Ala Ala Gly Ala Cys Cys Gly Thr Gly Ala Cys Ala Gly Cys Cys

1070 1075 1080

Ala Cys Ala Ala Cys Ala Gly Cys Thr Gly Ala Cys Gly Gly Cys

1085 1090 1095

Cys Ala Gly Ala Gly Ala Ala Thr Gly Cys Gly Gly Ala Gly Ala

1100 1105 1110

Cys Thr Gly Ala Ala Gly Thr Gly Cys Ala Ala Gly Gly Cys Cys

1115 1120 1125

Thr Gly Cys Cys Gly Gly Cys Thr Gly Ala Gly Ala Thr Gly Gly

1130 1135 1140

Gly Cys Thr Ala Gly Ala Cys Cys Thr Gly Ala Ala Cys Cys Thr

1145 1150 1155

Gly Cys Thr Gly Cys Thr Cys Ala Gly Gly Cys Cys Cys Cys Thr

1160 1165 1170

Cys Cys Ala Ala Ala Gly Ala Gly Cys Cys Ala Cys Thr Thr Thr

1175 1180 1185

Cys Cys Ala Cys Cys Thr Cys Ala Thr Cys Cys Thr Ala Gly Cys

1190 1195 1200

Gly Gly Cys Cys Cys Thr Ala Gly Cys Ala Gly Ala Ala Cys Cys

1205 1210 1215

Ala Gly Thr Thr Cys Thr Cys Thr Gly Cys Ala Gly Cys Thr Gly

1220 1225 1230

Cys Gly Gly Ala Gly Cys Ala Thr Cys Cys Cys Thr Cys Ala Cys

1235 1240 1245

Gly Thr Gly Cys Cys Ala Ala Ala Ala Gly Cys Cys Ala Cys Ala

1250 1255 1260

Gly Cys Cys Gly Cys Thr Cys Ala Cys Ala Gly Cys Gly Thr Gly

1265 1270 1275

Ala Cys Ala Gly Thr Gly Ala Ala Gly Cys Thr Gly Cys Ala Gly

1280 1285 1290

Ala Ala Gly Gly Gly Ala Ala Ala Gly Ala Ala Cys Ala Gly Cys

1295 1300 1305

Cys Cys Cys Thr Gly Cys Thr Gly Cys Cys Ala Ala Ala Ala Gly

1310 1315 1320

296

Ala Ala Ala Cys Thr Gly Cys Gly Gly Gly Thr Gly Cys Ala Gly

1325 1330 1335

Ala Ala Thr Cys Ala Gly Gly Gly Cys Cys Ala Thr Cys Thr Gly

1340 1345 1350

Cys Thr Gly Ala Thr Gly Ala Thr Cys Cys Thr Gly Cys Thr Gly

1355 1360 1365

Cys Ala Cys Ala Ala Thr Ala Cys Cys Thr Ala Cys Ala Thr Gly

1370 1375 1380

Gly Ala Ala Ala Thr Gly Cys Ala Cys Thr Ala Cys Gly Gly Cys

1385 1390 1395

Cys Ala Thr Cys Ala Gly Gly Cys Cys Cys Cys Ala Cys Ala Gly

1400 1405 1410

Gly Thr Gly Gly Cys Cys Ala Thr Gly Cys Thr Gly Cys Cys Thr

1415 1420 1425

Ala Ala Gly Cys Thr Gly Cys Thr Gly Thr Gly Cys Ala Gly Ala

1430 1435 1440

Cys Ala Gly Ala Thr Gly Cys Thr Gly Cys Cys Cys Ala Cys Thr

1445 1450 1455

Cys Thr Gly Ala Gly Cys Ala Cys Ala Cys Thr Gly Cys Ala Gly

1460 1465 1470

Cys Ala Ala Ala Gly Ala Cys Ala Cys Cys Cys Ala Cys Ala Gly

1475 1480 1485

Gly Gly Cys Cys Cys Thr Thr Gly Thr Cys Cys Thr Cys Cys Ala

1490 1495 1500

Cys Cys Thr Gly Thr Gly Ala Cys Ala Cys Cys Ala Cys Thr Gly

1505 1510 1515

Ala Gly Ala Gly Thr Cys Cys Ala Gly Thr Cys Thr Gly Thr Cys

1520 1525 1530

Cys Thr Gly Cys Thr Gly Cys Thr Gly Gly Gly Ala Gly Thr Gly

1535 1540 1545

Cys Cys Thr Cys Ala Gly Ala Cys Ala Gly Cys Thr Gly Cys Thr

1550 1555 1560

Ala Gly Ala Cys Ala Gly Cys Cys Thr Ala Gly Cys Ala Gly Ala

1565 1570 1575

Cys Cys Cys Ala Cys Cys Gly Gly Cys Ala Gly Ala Ala Cys Cys

1580 1585 1590

297

Cys Cys Thr Ala Cys Cys Ala Cys Ala Ala Gly Ala Cys Thr Gly

1595 1600 1605

Ala Thr Gly Gly Cys Cys Cys Cys Thr Gly Thr Cys Gly Gly Cys

1610 1615 1620

Ala Gly Cys Gly Thr Gly Ala Thr Gly Ala Gly Cys Thr Gly Thr

1625 1630 1635

Thr Cys Thr Cys Thr Gly Cys Thr Gly Ala Cys Cys Cys Ala Gly

1640 1645 1650

Cys Cys Thr Thr Cys Thr Cys Cys Ala Gly Cys Cys Ala Gly Cys

1655 1660 1665

Gly Cys Cys Thr Gly Thr Ala Cys Ala Ala Thr Gly Cys Cys Thr

1670 1675 1680

Cys Cys Ala Cys Thr Gly Cys Thr Gly Cys Ala Thr Cys Cys Thr

1685 1690 1695

Cys Ala Gly Thr Gly Gly Gly Cys Cys Cys Cys Ala Cys Ala Thr

1700 1705 1710

Thr Cys Thr Gly Cys Thr Gly Gly Ala Thr Thr Gly Thr Cys Thr

1715 1720 1725

Cys Cys Cys Gly Gly Cys Gly Cys Thr Thr Gly Cys Ala Gly Ala

1730 1735 1740

Cys Cys Thr Gly Cys Cys Thr Gly Thr Ala Cys Cys Thr Gly Thr

1745 1750 1755

Ala Thr Cys Ala Gly Cys Ala Thr Gly Ala Cys Cys Ala Cys Cys

1760 1765 1770

Ala Thr Cys Thr Cys Cys Ala Gly Ala Gly Cys Cys Ala Cys Cys

1775 1780 1785

Thr Gly Gly Cys Ala Gly Gly Thr Thr Thr Thr Cys Cys Gly Thr Gly

1790 1795 1800

Thr Ala Cys Ala Gly Cys Gly Gly Cys Ala Ala Thr Cys Thr Gly

1805 1810 1815

Cys Thr Gly Ala Gly Cys Thr Thr Cys Thr Cys Cys Ala Gly Ala

1820 1825 1830

Cys Gly Gly Gly Gly Cys Ala Thr Gly Ala Ala Gly Ala Gly Thr

1835 1840 1845

Ala Gly Cys Gly Thr Gly Cys Gly Gly Cys Thr Gly Ala Ala Ala

1850 1855 1860

Ala Ala Cys Ala Gly Cys Cys Gly Gly Cys Gly Gly Ala Ala Cys

298

1865 1870 1875

Ala Gly Ala Cys Gly Gly Thr Ala Cys Ala Gly Ala Ala Ala Gly

1880 1885 1890

Ala Thr Cys Thr Ala Cys Gly Gly Cys Gly Ala Gly Cys Thr Gly

1895 1900 1905

Ala Ala Gly Ala Thr Gly Thr Cys Cys Cys Thr Gly Cys Ala Gly

1910 1915 1920

Gly Cys Cys Gly Thr Gly Gly Cys Thr Cys Cys Thr Ala Gly Ala

1925 1930 1935

Thr Gly Thr Cys Cys Thr Ala Gly Ala Thr Cys Thr Gly Cys Cys

1940 1945 1950

Gly Thr Gly Gly Ala Cys Ala Thr Cys Ala Gly Cys Cys Cys Cys

1955 1960 1965

Gly Gly Ala Cys Cys Thr Ala Cys Cys Thr Cys Thr Cys Cys Thr

1970 1975 1980

Ala Gly Cys Cys Thr Gly Gly Cys Cys Thr Cys Thr Thr Gly Thr

1985 1990 1995

Gly Cys Cys Thr Cys Thr Gly Thr Gly Gly Cys Cys Ala Thr Thr

2000 2005 2010

Gly Gly Ala Ala Ala Ala Cys Thr Gly Gly Cys Cys Cys Thr Gly

2015 2020 2025

Cys Thr Thr Gly Gly Cys Gly Cys Thr Thr Cys Thr Gly Cys Cys

2030 2035 2040

Cys Cys Thr Thr Gly Thr Gly Gly Thr Gly Cys Thr Gly Cys Thr

2045 2050 2055

Gly Gly Cys Gly Cys Thr Ala Gly Ala Gly Ala Gly Gly Cys Thr

2060 2065 2070

Gly Gly Ala Ala Gly Ala Thr Thr Thr Gly Cys Cys Gly Thr Gly

2075 2080 2085

Cys Thr Gly Gly Gly Cys Ala Cys Cys Ala Thr Gly Gly Thr Cys

2090 2095 2100

Ala Thr Gly Ala Ala Gly Cys Ala Cys Cys Thr Gly Ala Ala Cys

2105 2110 2115

Cys Gly Cys Ala Ala Gly Cys Thr Gly Cys Ala Thr Cys Thr Gly

2120 2125 2130

Ala Cys Cys Cys Gly Gly Thr Cys Cys Thr Gly Cys Cys Thr Gly

2135 2140 2145

299

Ala Thr Cys Gly Ala Thr Cys Thr Gly Ala Cys Cys Ala Thr Gly

2150 2155 2160

Ala Thr Gly Ala Thr Gly Ala Gly Cys Cys Ala Gly Ala Ala Gly

2165 2170 2175

Cys Thr Cys Thr Gly Gly Ala Ala Gly Ala Ala Cys Cys Ala Gly

2180 2185 2190

Cys Gly Gly Ala Ala Gly Ala Gly Ala Ala Thr Cys Cys Cys Thr

2195 2200 2205

Cys Thr Gly Cys Cys Ala Thr Cys Thr Cys Cys Thr Ala Gly Ala

2210 2215 2220

Cys Ala Gly Cys Ala Thr Cys Thr Gly Cys Thr Cys Cys Cys Thr

2225 2230 2235

Cys Ala Gly Thr Ala Cys Cys Cys Thr Cys Thr Gly Cys Ala Cys

2240 2245 2250

Cys Cys Thr Cys Cys Thr Cys Cys Ala Cys Cys Thr Thr Gly Thr

2255 2260 2265

Cys Thr Gly Cys Thr Gly Cys Thr Gly Cys Thr Cys Cys Ala Cys

2270 2275 2280

Cys Ala Cys Ala Gly Ala Cys Ala Cys Cys Ala Cys Cys Thr Gly

2285 2290 2295

Gly Cys Cys Thr Thr Thr Thr Cys Thr Gly Gly Ala Ala Ala Thr Gly

2300 2305 2310

Gly Cys Cys Thr Gly Cys Ala Gly Cys Ala Gly Cys Cys Ala Gly

2315 2320 2325

Cys Ala Cys Ala Cys Cys Cys Ala Cys Thr Cys Cys Ala Thr Cys

2330 2335 2340

Ala Ala Gly Ala Thr Cys Thr Gly Gly Ala Thr Cys Ala Gly Cys

2345 2350 2355

Thr Thr Thr Ala Gly Cys Cys Ala Cys Gly Ala Gly Cys Ala Gly

2360 2365 2370

Ala Gly Cys Ala Gly Cys Cys Cys Ala Thr Thr Cys Cys Thr Gly

2375 2380 2385

Gly Cys Cys Ala Gly Ala Thr Cys Cys Thr Gly Gly Ala Ala Cys

2390 2395 2400

Ala Cys Cys Ala Thr Gly Cys Ala Gly Thr Gly Gly Ala Ala Cys

2405 2410 2415

300

Ala Ala Cys Ala Ala Gly Ala Ala Ala Ala Thr Gly Gly Cys Cys

2420 2425 2430

Ala Thr Gly Ala Cys Ala Gly Thr Gly Ala Thr Cys Gly Cys Thr

2435 2440 2445

Gly Gly Cys Gly Cys Cys Cys Ala Cys Cys Ala Cys Thr Gly Thr

2450 2455 2460

Cys Ala Gly Thr Ala Cys Ala Ala Gly Cys Thr Gly Ala Cys Cys

2465 2470 2475

Thr Ala Cys Ala Cys Cys Ala Ala Ala Gly Thr Gly Thr Gly Gly

2480 2485 2490

Ala Ala Gly Ala Thr Gly Ala Ala Thr Thr Gly Gly Ala Ala Gly

2495 2500 2505

Ala Ala Ala Ala Thr Gly Ala Thr Gly Ala Thr Cys Gly Gly Cys

2510 2515 2520

Thr Cys Cys Cys Ala Gly Cys Ala Cys Cys Thr Cys Cys Thr Gly

2525 2530 2535

Thr Thr Cys Ala Gly Cys Cys Cys Thr Cys Thr Gly Gly Ala Thr

2540 2545 2550

Ala Gly Cys Thr Cys Thr Cys Cys Ala Cys Thr Gly Cys Ala Ala

2555 2560 2565

Cys Thr Gly Cys Thr Gly Cys Thr Thr Cys Ala Cys Cys Cys Thr

2570 2575 2580

Cys Ala Cys Gly Gly Cys Ala Ala Gly Ala Gly Ala Thr Gly Cys

2585 2590 2595

Ala Ala Cys Cys Cys Thr Thr Gly Cys Thr Gly Cys Ala Gly Ala

2600 2605 2610

Cys Thr Gly Ala Cys Ala Thr Gly Gly Ala Thr Gly Thr Cys Cys

2615 2620 2625

Ala Thr Gly Cys Thr Gly Cys Ala Gly Ala Ala Ala Cys Ala Gly

2630 2635 2640

Thr Gly Cys Thr Gly Cys Thr Ala Cys Ala Ala Cys Cys Thr Cys

2645 2650 2655

Gly Thr Gly Ala Thr Cys Ala Ala Gly Cys Ala Cys Cys Cys Ala

2660 2665 2670

Cys Thr Gly Ala Thr Cys Thr Thr Cys Ala Gly Ala Ala Thr Cys

2675 2680 2685

301

Ala Ala Cys Cys Thr Gly Cys Gly Gly Ala Gly Ala Cys Ala Gly

2690 2695 2700

Gly Gly Cys Ala Ala Gly Ala Cys Cys Ala Ala Gly Gly Ala Thr

2705 2710 2715

Gly Gly Cys Gly Thr Thr Gly Thr Gly Cys Ala Gly Ala Gly Cys

2720 2725 2730

Cys Thr Gly Gly Gly Cys Thr Thr Cys Cys Thr Gly Ala Ala Cys

2735 2740 2745

Cys Ala Gly Cys Ala Gly Ala Thr Cys Ala Thr Cys Cys Thr Gly

2750 2755 2760

Cys Ala Gly Ala Ala Ala Gly Ala Gly Thr Thr Cys Thr Thr Cys

2765 2770 2775

Ala Thr Cys Thr Gly Cys Cys Thr Gly Cys Thr Cys Ala Thr Gly

2780 2785 2790

Thr Thr Thr Ala Thr Cys Ala Ala Gly Cys Gly Gly Thr Gly Gly

2795 2800 2805

Ala Ala Gly Cys Cys Thr Gly Cys Cys Gly Cys Cys Ala Cys Cys

2810 2815 2820

Ala Ala Gly Ala Gly Ala Gly Ala Gly Thr Gly Gly Gly Ala Gly

2825 2830 2835

Ala Ala Ala Cys Gly Cys Ala Ala Gly Cys Ala Gly Ala Thr Cys

2840 2845 2850

Cys Thr Cys Gly Gly Cys Gly Gly Ala Gly Gly Cys Gly Gly Cys

2855 2860 2865

Ala Ala Gly Thr Ala Cys Ala Ala Ala Gly Ala Gly Thr Ala Cys

2870 2875 2880

Thr Thr Cys Cys Thr Gly Ala Ala Gly Cys Gly Cys Ala Thr Cys

2885 2890 2895

Cys Thr Gly Ala Thr Thr Cys Gly Gly Ala Ala Gly Gly Cys Cys

2900 2905 2910

Ala Thr Gly Ala Cys Thr Gly Thr Gly Cys Thr Gly Gly Cys Cys

2915 2920 2925

Gly Gly Cys Gly Ala Cys Ala Ala Gly Ala Ala Ala Gly Gly Cys

2930 2935 2940

Cys Thr Gly Gly Gly Cys Ala Gly Ala Thr Thr Cys Ala Thr Gly

2945 2950 2955

Ala Gly Ala Thr Gly Cys Gly Thr Gly Cys Ala Gly Ala Gly Cys

302

2960 2965 2970

Gly Ala Gly Ala Cys Ala Ala Ala Gly Gly Cys Cys Gly Thr Thr

2975 2980 2985

Ala Gly Cys Cys Thr Gly Cys Ala Gly Cys Thr Cys Cys Cys Ala

2990 2995 3000

Cys Thr Cys Gly Gly Ala Ala Gly Ala Gly Gly Cys Cys Cys Thr

3005 3010 3015

Gly Thr Gly Thr Ala Cys Thr Ala Cys Gly Thr Gly Thr Gly Cys

3020 3025 3030

Ala Ala Cys Cys Gly Gly Ala Thr Gly Ala Ala Cys Gly Thr Gly

3035 3040 3045

Thr Cys Cys Ala Gly Cys Gly Thr Gly Cys Thr Gly Ala Ala Gly

3050 3055 3060

Cys Cys Cys Thr Thr Cys Cys Thr Gly Thr Thr Cys Ala Cys Cys

3065 3070 3075

Gly Thr Gly Cys Cys Thr Ala Thr Cys Gly Ala Gly Gly Ala Cys

3080 3085 3090

Ala Thr Gly Ala Gly Ala Gly Ala Gly Thr Gly Cys Ala Cys Ala

3095 3100 3105

Cys Thr Gly Gly Gly Cys Cys Gly Cys Ala Ala Cys Ala Gly Ala

3110 3115 3120

Ala Thr Gly Ala Ala Thr Gly Thr Gly Thr Cys Cys Thr Cys Thr

3125 3130 3135

Gly Thr Gly Cys Thr Gly Ala Ala Ala Cys Cys Cys Thr Gly Thr

3140 3145 3150

Cys Thr Gly Ala Thr Cys Cys Cys Thr Gly Thr Gly Cys Cys Ala

3155 3160 3165

Ala Thr Cys Gly Ala Cys Cys Thr Gly Cys Cys Thr Cys Gly Gly

3170 3175 3180

Ala Thr Gly Cys Thr Gly Cys Cys Ala Ala Thr Cys Ala Gly Cys

3185 3190 3195

Ala Gly Cys Cys Cys Ala Cys Thr Gly Cys Ala Cys Ala Gly Ala

3200 3205 3210

Cys Cys Cys Cys Ala Gly Ala Thr Gly Cys Cys Ala Thr Thr Cys

3215 3220 3225

Thr Gly Gly Ala Cys Cys Thr Gly Cys Ala Cys Cys Thr Thr Thr

3230 3235 3240

303

Cys Cys Thr Gly Cys Cys Ala Cys Ala Ala Cys Ala Thr Gly Gly

3245 3250 3255

Gly Gly Cys Ala Cys Cys Thr Gly Gly Gly Ala Gly Ala Cys Ala

3260 3265 3270

Cys Cys Thr Thr Cys Thr Ala Cys Ala Thr Gly Gly Gly Gly Cys

3275 3280 3285

Thr Gly Gly Cys Gly Gly Ala Cys Cys Thr Thr Thr Thr Cys Gly Gly

3290 3295 3300

Gly Gly Cys Cys Cys Thr Ala Gly Ala Ala Gly Ala Ala Ala Ala

3305 3310 3315

Gly Thr Gly Cys Thr Gly Ala Thr Gly Gly Thr Gly Cys Thr Gly

3320 3325 3330

Thr Gly Gly Cys Thr Gly Cys Cys Cys Gly Cys Cys Ala Ala Cys

3335 3340 3345

Cys Ala Gly Ala Gly Thr Cys Thr Gly Cys Ala Gly Ala Ala Ala

3350 3355 3360

Ala Gly Cys Cys Ala Ala Gly Thr Gly Thr Thr Cys Cys Ala Gly

3365 3370 3375

Thr Gly Gly Gly Thr Cys Cys Thr Gly Cys Gly Gly Ala Cys Cys

3380 3385 3390

Gly Ala Gly Ala Ala Cys Ala Thr Cys Cys Gly Gly Ala Ala Gly

3395 3400 3405

Cys Thr Gly Ala Thr Cys Thr Gly Gly Ala Cys Cys Ala Ala Gly

3410 3415 3420

Cys Ala Gly Cys Gly Gly Ala Gly Cys Thr Thr Cys Cys Thr Gly

3425 3430 3435

Thr Cys Thr Cys Thr Gly Thr Thr Cys Cys Cys Cys Ala Ala Gly

3440 3445 3450

Cys Thr Gly Ala Gly Ala Gly Cys Cys Ala Cys Cys Gly Ala Ala

3455 3460 3465

Cys Ala Gly Ala Thr Gly Ala Cys Cys Ala Ala Thr Gly Thr Gly

3470 3475 3480

Gly Gly Cys Thr Gly Cys Gly Cys Cys Thr Thr Cys Ala Gly Cys

3485 3490 3495

Gly Gly Cys Cys Gly Gly Ala Thr Thr Cys Thr Gly Cys Ala Thr

3500 3505 3510

304

Cys Ala Ala Cys Cys Thr Gly Ala Ala Ala Thr Gly Gly Cys Cys

3515 3520 3525

Gly Cys Cys Ala Thr Gly Cys Gly Gly Cys Thr Gly Ala Thr Gly

3530 3535 3540

Cys Cys Cys Ala Ala Gly Thr Thr Cys Cys Thr Gly Ala Ala Thr

3545 3550 3555

Ala Gly Cys Ala Cys Cys Ala Ala Cys Ala Gly Cys Thr Gly Gly

3560 3565 3570

Thr Gly Gly Ala Cys Cys Thr Gly Thr Ala Cys Cys Ala Ala Cys

3575 3580 3585

Cys Thr Gly Ala Gly Cys Gly Thr Gly Cys Cys Cys Ala Thr Gly

3590 3595 3600

Ala Thr Gly Cys Thr Gly Ala Cys Cys Ala Thr Thr Cys Thr Gly

3605 3610 3615

Ala Thr Thr Thr Gly Gly Ala Ala Gly Cys Gly Gly Gly Thr Gly

3620 3625 3630

Thr Thr Cys Ala Thr Thr Cys Thr Cys Cys Thr Gly Cys Thr Gly

3635 3640 3645

Thr Cys Cys Gly Ala Cys Ala Ala Gly Ala Ala Gly Cys Thr Gly

3650 3655 3660

Cys Thr Gly Ala Ala Gly Ala Ala Gly Thr Cys Thr Ala Gly Cys

3665 3670 3675

Cys Ala Gly Cys Thr Gly Ala Gly Gly Cys Gly Gly Thr Cys Thr

3680 3685 3690

Thr Gly Gly Ala Gly Ala Ala Ala Cys Gly Thr Gly Thr Cys Cys

3695 3700 3705

Cys Gly Gly Ala Thr Cys Ala Gly Cys Cys Ala Cys Cys Ala Gly

3710 3715 3720

Ala Thr Gly Ala Ala Gly Thr Gly Thr Cys Thr Gly Ala Cys Cys

3725 3730 3735

Gly Cys Cys Gly Thr Gly Gly Ala Cys Thr Gly Thr Cys Thr Gly

3740 3745 3750

Cys Ala Gly Gly Cys Cys Thr Thr Cys Thr Gly Gly Gly Ala Cys

3755 3760 3765

Ala Thr Gly Ala Ala Cys Ala Thr Gly Gly Ala Ala Cys Ala Cys

3770 3775 3780

305

Cys Gly Gly Cys Thr Gly Ala Cys Cys Ala Thr Gly Gly Ala Cys

3785 3790 3795

Ala Gly Cys Gly Cys Cys Cys Thr Gly Ala Gly Cys Cys Thr Gly

3800 3805 3810

Ala Cys Ala Ala Ala Gly Ala Gly Ala Gly Ala Gly Gly Gly Ala

3815 3820 3825

Cys Cys Cys Gly Ala Gly Ala Cys Thr Thr Gly Cys Ala Ala Gly

3830 3835 3840

Ala Ala Gly Ala Ala Gly Cys Thr Cys Cys Thr Gly Cys Thr Gly

3845 3850 3855

Ala Ala Ala Cys Thr Gly Cys Thr Gly Ala Cys Ala Ala Thr Gly

3860 3865 3870

Ala Gly Cys Cys Thr Gly Ala Gly Cys Ala Ala Cys Ala Thr Cys

3875 3880 3885

Cys Thr Gly Ala Thr Gly Ala Ala Gly Thr Thr Cys Cys Gly Gly

3890 3895 3900

Cys Thr Gly Cys Gly Gly Cys Thr Gly Ala Gly Ala Ala Thr Cys

3905 3910 3915

Cys Cys Ala Ala Gly Cys Cys Thr Gly Ala Gly Gly Ala Ala Gly

3920 3925 3930

Thr Ala Thr Cys Gly Cys Cys Ala Gly Cys Ala Thr Cys Thr Gly

3935 3940 3945

Ala Ala Ala Ala Ala Gly Ala Cys Cys Ala Gly Cys Gly Cys Cys

3950 3955 3960

Ala Ala Gly Gly Gly Cys Gly Ala Gly Ala Ala Gly Cys Thr Gly

3965 3970 3975

Ala Ala Gly Cys Ala Cys Thr Gly Cys Thr Thr Thr Ala Ala Gly

3980 3985 3990

Gly Cys Thr Thr Gly Gly Gly Gly Cys Ala Gly Ala Gly Gly Cys

3995 4000 4005

Cys Cys Ala Cys Cys Thr Cys Thr Gly Cys Ala Cys Ala Cys Ala

4010 4015 4020

Ala Gly Ala Ala Gly Cys Cys Ala Gly Ala Ala Gly Ala Ala Ala

4025 4030 4035

Gly Ala Cys Cys Cys Thr Gly Cys Cys Gly Gly Cys Ala Ala Gly

4040 4045 4050

Cys Thr Gly Ala Ala Ala Ala Ala Ala Cys Thr Gly Ala Cys Cys

306

4055 4060 4065

Ala Cys Ala Cys Thr Gly Ala Gly Cys Thr Thr Cys Gly Thr Gly

4070 4075 4080

Cys Thr Gly Ala Thr Cys Gly Ala Gly Gly Ala Ala Cys Gly Gly

4085 4090 4095

Cys Gly Gly Ala Ala Cys Cys Thr Gly Cys Ala Gly Cys Gly Gly

4100 4105 4110

Ala Ala Thr Cys Thr Gly Ala Ala Ala Gly Thr Cys Cys Thr Gly

4115 4120 4125

Thr Gly Gly Cys Thr Cys Ala Ala Gly Ala Ala Ala Ala Gly Cys

4130 4135 4140

Ala Cys Cys Ala Ala Gly Ala Gly Cys Cys Gly Gly Thr Ala Thr

4145 4150 4155

Cys Thr Gly Thr Thr Cys Thr Thr Cys Cys Thr Gly Ala Cys Cys

4160 4165 4170

Gly Thr Gly Thr Gly Cys Gly Gly Cys Thr Thr Cys Thr Thr Cys

4175 4180 4185

Gly Ala Gly Ala Ala Gly Ala Ala Ala Cys Gly Gly Cys Cys Thr

4190 4195 4200

Gly Ala Gly Thr Gly Gly Cys Thr Gly Thr Cys Cys Thr Cys Cys

4205 4210 4215

Ala Gly Ala Ala Ala Gly Ala Ala Gly Gly Gly Ala Cys Ala Cys

4220 4225 4230

Ala Cys Ala Ala Ala Gly Ala Ala Thr Cys Gly Gly Ala Gly Gly

4235 4240 4245

Ala Ala Gly Gly Cys Cys Ala Ala Gly Cys Gly Gly Ala Ala Gly

4250 4255 4260

Cys Cys Cys Cys Ala Gly Ala Thr Cys Gly Thr Gly Cys Ala Cys

4265 4270 4275

Ala Ala Gly Ala Ala Gly Ala Gly Ala Gly Gly Cys Cys Thr Gly

4280 4285 4290

Thr Thr Cys Cys Thr Cys Ala Ala Ala Ala Ala Gly Ala Ala Ala

4295 4300 4305

Thr Ala Cys Cys Thr Gly Gly Thr Gly Cys Ala Gly Gly Gly Cys

4310 4315 4320

Gly Cys Cys Ala Ala Gly Ala Ala Gly Ala Thr Cys Ala Cys Cys

4325 4330 4335

307

Thr Thr Cys Cys Thr Gly Thr Cys Cys Ala Ala Cys Gly Gly Cys

4340 4345 4350

Ala Thr Gly Gly Ala Ala Gly Cys Thr Thr Gly Cys Gly Gly Cys

4355 4360 4365

Ala Cys Ala Cys Ala Cys Cys Cys Cys Ala Cys Ala Cys Cys Ala

4370 4375 4380

Ala Gly Ala Cys Thr Gly Ala Gly Cys Gly Ala Cys Ala Gly Ala

4385 4390 4395

Gly Thr Gly Thr Thr Cys Cys Thr Gly Gly Thr Cys Ala Ala Gly

4400 4405 4410

Ala Thr Cys Ala Cys Cys Ala Cys Cys Ala Cys Cys Ala Cys Ala

4415 4420 4425

Ala Gly Cys Ala Gly Cys Cys Cys Cys Ala Gly Ala Ala Gly Ala

4430 4435 4440

Ala Gly Cys Cys Cys Ala Gly Cys Ala Cys Thr Gly Ala Gly Ala

4445 4450 4455

Gly Cys Thr Ala Gly Ala Gly Gly Cys Gly Gly Cys Ala Cys Cys

4460 4465 4470

Ala Cys Ala Ala Thr Thr Gly Gly Cys Gly Ala Ala Gly Thr Gly

4475 4480 4485

Ala Cys Cys Ala Gly Cys Cys Thr Gly Thr Gly Cys Thr Thr Cys

4490 4495 4500

Thr Thr Cys Thr Ala Cys Cys Thr Gly Cys Thr Gly Thr Ala Cys

4505 4510 4515

<210> 1099

<211> 4521

<212> PROTEIN

<213> Artificial sequence

<220>

<223> polynucleotide 4A

<400> 1099

Ala Thr Cys Cys Ala Gly Ala Ala Gly Ala Ala Gly Ala Ala Ala Cys

1 5 10 15

Ala Gly Ala Ala Ala Thr Cys Cys Ala Thr Gly Gly Cys Cys Ala Ala

20 25 30

Gly Ala Ala Gly Thr Cys Cys Cys Ala Gly Cys Ala Gly Cys Cys Thr

35 40 45

Cys Thr Gly Gly Thr Gly Cys Thr Gly Ala Ala Gly Ala Ala Gly Ala

308

50 55 60

Ala Cys Cys Gly Gly Ala Ala Gly Cys Ala Ala Gly Ala Gly Thr Gly

65 70 75 80

Gly Gly Gly Ala Gly Gly Ala Cys Ala Gly Gly Cys Cys Cys Ala Cys

85 90 95

Ala Gly Ala Cys Ala Gly Ala Gly Ala Ala Ala Cys Ala Ala Cys Gly

100 105 110

Cys Cys Ala Ala Gly Ala Ala Gly Ala Thr Gly Cys Cys Cys Thr Thr

115 120 125

Cys Cys Ala Ala Gly Ala Gly Gly Ala Ala Ala Ala Gly Ala Ala Gly

130 135 140

Ala Ala Cys Gly Gly Cys Cys Gly Gly Ala Thr Cys Ala Gly Cys Ala

145 150 155 160

Gly Cys Ala Gly Cys Cys Thr Gly Ala Thr Cys Cys Thr Gly Ala Gly

165 170 175

Cys Thr Thr Cys Cys Ala Gly Ala Gly Ala Gly Thr Gly Ala Ala Gly

180 185 190

Gly Ala Cys Ala Gly Cys Ala Ala Gly Ala Ala Ala Ala Thr Gly Ala

195 200 205

Cys Cys Cys Ala Gly Ala Ala Gly Ala Thr Gly Thr Gly Cys Ala Gly

210 215 220

Cys Thr Cys Thr Cys Thr Gly Gly Cys Cys Cys Thr Gly Cys Cys Thr

225 230 235 240

Ala Cys Ala Gly Gly Cys Cys Thr Gly Ala Cys Ala Thr Cys Thr Cys

245 250 255

Thr Gly Ala Thr Cys Cys Thr Gly Cys Cys Thr Ala Gly Cys Ala Gly

260 265 270

Cys Gly Ala Thr Cys Thr Gly Gly Cys Cys Gly Thr Gly Cys Thr Gly

275 280 285

Ala Thr Cys Thr Cys Thr Ala Gly Cys Ala Gly Cys Ala Cys Cys Ala

290 295 300

Gly Cys Cys Ala Cys Thr Thr Thr Cys Thr Gly Ala Thr Gly Cys Gly

305 310 315 320

Gly Ala Gly Cys Cys Cys Thr Gly Thr Gly Cys Thr Gly Cys Cys Ala

325 330 335

Ala Gly Cys Thr Cys Cys Ala Gly Ala Cys Thr Gly Ala Cys Ala Thr

340 345 350

309

Gly Thr Gly Cys Cys Thr Cys Thr Cys Cys Ala Cys Ala Gly Cys Thr

355 360 365

Gly Cys Cys Cys Cys Gly Gly Ala Thr Gly Thr Gly Gly Ala Cys Ala

370 375 380

Thr Gly Gly Thr Cys Thr Ala Gly Cys Thr Gly Gly Cys Thr Gly Ala

385 390 395 400

Ala Gly Cys Ala Cys Cys Cys Cys Ala Gly Ala Ala Cys Cys Gly Cys

405 410 415

Thr Ala Gly Cys Ala Thr Cys Ala Cys Ala Cys Thr Gly Cys Cys Thr

420 425 430

Ala Cys Cys Ala Cys Ala Ala Gly Cys Cys Thr Gly Ala Ala Gly Gly

435 440 445

Gly Cys Ala Gly Cys Ala Gly Ala Ala Cys Cys Ala Gly Cys Thr Gly

450 455 460

Cys Thr Gly Cys Cys Cys Ala Ala Gly Ala Thr Cys Thr Cys Ala Cys

465 470 475 480

Cys Cys Cys Ala Gly Ala Cys Thr Gly Cys Cys Thr Ala Gly Gly Ala

485 490 495

Cys Cys Ala Ala Gly Thr Gly Gly Cys Cys Thr Cys Thr Gly Cys Ala

500 505 510

Gly Thr Gly Thr Cys Thr Gly Cys Thr Gly Cys Ala Cys Ala Gly Cys

515 520 525

Cys Thr Gly Gly Gly Ala Ala Cys Ala Ala Cys Ala Gly Gly Cys Ala

530 535 540

Cys Ala Cys Thr Cys Gly Thr Thr Thr Gly Gly Gly Ala Ala Ala Gly

545 550 555 560

Gly Gly Gly Cys Gly Thr Gly Cys Thr Gly Cys Ala Gly Ala Thr Gly

565 570 575

Ala Gly Ala Ala Gly Cys Cys Thr Cys Gly Ala Ala Gly Cys Cys Cys

580 585 590

Ala Thr Thr Gly Thr Cys Cys Thr Cys Thr Gly Cys Ala Gly Ala Cys

595 600 605

Cys Thr Thr Cys Cys Ala Thr Cys Thr Gly Cys Ala Cys Cys Ala Cys

610 615 620

Thr Gly Cys Ala Gly Ala Ala Cys Cys Cys Ala Gly Cys Cys Ala Ala

625 630 635 640

310

Gly Cys Thr Cys Thr Thr Gly Thr Cys Cys Thr Cys Cys Thr Gly Cys

645 650 655

Cys Thr Gly Gly Ala Thr Thr Cys Thr Gly Thr Ala Cys Cys Ala Ala

660 665 670

Gly Gly Cys Cys Ala Gly Thr Gly Gly Ala Gly Cys Gly Gly Ala Gly

675 680 685

Cys Cys Gly Thr Cys Gly Thr Thr Gly Gly Ala Cys Thr Thr Ala Gly

690 695 700

Ala Cys Cys Cys Gly Thr Gly Gly Cys Thr Cys Ala Cys Cys Ala Thr

705 710 715 720

Cys Thr Gly Cys Cys Thr Ala Gly Ala Cys Cys Thr Ala Thr Cys Cys

725 730 735

Ala Gly Gly Gly Cys Cys Thr Gly Cys Ala Cys Thr Gly Thr Cys Cys

740 745 750

Thr Cys Ala Gly Gly Cys Thr Cys Cys Thr Cys Ala Thr Cys Ala Ala

755 760 765

Cys Cys Thr Gly Cys Thr Cys Thr Gly Ala Gly Cys Thr Gly Gly Gly

770 775 780

Gly Ala Ala Ala Gly Thr Gly Gly Cys Cys Cys Gly Thr Gly Gly Gly

785 790 795 800

Ala Ala Thr Gly Ala Cys Ala Gly Gly Ala Cys Cys Thr Gly Cys Cys

805 810 815

Ala Gly Ala Thr Gly Cys Thr Gly Thr Cys Gly Gly Thr Gly Gly Cys

820 825 830

Cys Cys Cys Gly Cys Ala Gly Ala Thr Cys Thr Cys Ala Gly Ala Ala

835 840 845

Gly Thr Cys Thr Cys Ala Gly Cys Ala Thr Cys Ala Cys Thr Gly Cys

850 855 860

Gly Cys Cys Cys Thr Gly Cys Gly Gly Ala Ala Gly Ala Thr Cys Gly

865 870 875 880

Ala Gly Thr Gly Gly Gly Cys Cys Ala Cys Ala Ala Ala Ala Cys Thr

885 890 895

Gly Thr Cys Thr Cys Thr Cys Gly Gly Cys Gly Gly Ala Ala Ala Ala

900 905 910

Gly Cys Cys Gly Gly Ala Cys Cys Ala Cys Ala Gly Gly Cys Thr Cys

915 920 925

311

Ala Ala Gly Ala Gly Ala Ala Gly Gly Cys Cys Ala Gly Ala Cys Cys

930 935 940

Thr Thr Cys Thr Cys Cys Thr Thr Gly Cys Cys Thr Gly Thr Cys Cys

945 950 955 960

Thr Gly Gly Thr Cys Cys Ala Gly Cys Ala Gly Ala Ala Thr Thr Gly

965 970 975

Thr Gly Gly Cys Cys Cys Cys Thr Gly Gly Ala Cys Ala Cys Gly Cys

980 985 990

Cys Cys Thr Gly Ala Gly Cys Thr Gly Thr Cys Gly Gly Ala Cys Thr

995 1000 1005

Cys Gly Thr Ala Gly Ala Ala Gly Ala Thr Gly Gly Thr Cys Cys

1010 1015 1020

Thr Cys Cys Ala Ala Gly Thr Gly Cys Ala Gly Ala Gly Gly Cys

1025 1030 1035

Gly Gly Cys Cys Cys Thr Ala Gly Cys Gly Cys Cys Ala Gly Cys

1040 1045 1050

Gly Ala Thr Ala Gly Ala Thr Gly Thr Thr Gly Thr Ala Gly Cys

1055 1060 1065

Thr Gly Thr Ala Gly Cys Cys Cys Cys Ala Gly Cys Gly Gly Cys

1070 1075 1080

Thr Thr Cys Ala Gly Cys Gly Cys Cys Gly Gly Ala Ala Gly Ala

1085 1090 1095

Gly Gly Cys Ala Gly Ala Thr Gly Thr Cys Cys Thr Gly Thr Gly

1100 1105 1110

Cys Ala Gly Gly Gly Ala Thr Gly Thr Cys Thr Gly Ala Gly Gly

1115 1120 1125

Cys Cys Ala Cys Ala Cys Ala Gly Ala Gly Thr Thr Cys Ala Gly

1130 1135 1140

Cys Thr Gly Cys Thr Gly Ala Gly Ala Ala Gly Ala Thr Gly Gly

1145 1150 1155

Gly Gly Cys Cys Cys Ala Gly Gly Ala Thr Cys Thr Cys Cys Thr

1160 1165 1170

Gly Cys Thr Gly Gly Cys Cys Ala Gly Ala Gly Ala Cys Thr Gly

1175 1180 1185

Thr Cys Thr Ala Ala Gly Gly Gly Cys Thr Thr Cys Cys Ala Ala

1190 1195 1200

Cys Thr Gly Cys Thr Gly Cys Gly Thr Thr Gly Gly Thr Gly Gly

312

1205 1210 1215

Gly Gly Ala Cys Cys Thr Gly Gly Ala Thr Cys Thr Cys Cys Ala

1220 1225 1230

Gly Cys Thr Cys Cys Thr Gly Ala Gly Cys Cys Thr Ala Gly Ala

1235 1240 1245

Ala Ala Gly Gly Gly Cys Cys Cys Ala Thr Thr Thr Cys Cys Thr

1250 1255 1260

Cys Cys Ala Cys Cys Thr Gly Ala Thr Cys Cys Thr Cys Cys Ala

1265 1270 1275

Thr Gly Gly Cys Cys Thr Gly Thr Gly Ala Cys Cys Gly Cys Thr

1280 1285 1290

Gly Thr Gly Ala Cys Ala Gly Thr Gly Ala Thr Gly Gly Cys Thr

1295 1300 1305

Gly Gly Cys Thr Cys Thr Gly Thr Gly Cys Cys Thr Ala Gly Cys

1310 1315 1320

Gly Cys Ala Cys Ala Gly Thr Cys Thr Gly Thr Gly Gly Ala Thr

1325 1330 1335

Gly Cys Cys Cys Thr Gly Gly Ala Ala Thr Cys Thr Cys Cys Thr

1340 1345 1350

Gly Gly Ala Cys Cys Thr Cys Thr Gly Gly Cys Thr Cys Thr Gly

1355 1360 1365

Gly Ala Ala Gly Gly Cys Cys Cys Thr Ala Gly Cys Ala Gly Cys

1370 1375 1380

Cys Cys Thr Ala Gly Ala Ala Ala Cys Cys Thr Gly Cys Thr Gly

1385 1390 1395

Thr Gly Gly Cys Gly Gly Gly Ala Ala Ala Thr Gly Ala Gly Cys

1400 1405 1410

Ala Thr Cys Thr Thr Thr Cys Thr Gly Cys Cys Cys Gly Gly Cys

1415 1420 1425

Ala Thr Cys Thr Thr Cys Thr Gly Cys Cys Ala Ala Ala Ala Gly

1430 1435 1440

Ala Ala Gly Gly Thr Gly Cys Cys Ala Thr Cys Thr Gly Cys Cys

1445 1450 1455

Ala Gly Cys Cys Cys Cys Thr Gly Gly Gly Gly Ala Cys Thr Thr

1460 1465 1470

Gly Thr Thr Cys Cys Thr Cys Cys Thr Thr Cys Thr Cys Cys Ala

1475 1480 1485

313

Cys Cys Ala Cys Thr Cys Gly Cys Thr Cys Thr Gly Gly Gly Cys

1490 1495 1500

Cys Cys Thr Ala Gly Ala Ala Thr Gly Cys Ala Gly Cys Thr Gly

1505 1510 1515

Thr Gly Thr Ala Cys Cys Cys Ala Gly Cys Thr Cys Gly Cys Cys

1520 1525 1530

Ala Gly Ala Thr Thr Cys Thr Thr Cys Cys Cys Cys Ala Thr Cys

1535 1540 1545

Ala Cys Ala Cys Cys Thr Cys Cys Ala Gly Thr Gly Thr Gly Gly

1550 1555 1560

Cys Ala Cys Ala Thr Cys Cys Thr Gly Gly Gly Ala Cys Cys Thr

1565 1570 1575

Cys Ala Gly Ala Gly Ala Cys Ala Cys Ala Cys Ala Cys Cys Thr

1580 1585 1590

Cys Ala Gly Gly Thr Gly Gly Thr Cys Cys Thr Gly Ala Gly Ala

1595 1600 1605

Cys Thr Gly Gly Gly Cys Ala Ala Cys Cys Thr Gly Cys Cys Thr

1610 1615 1620

Cys Cys Ala Thr Thr Cys Thr Thr Cys Ala Gly Ala Gly Gly Cys

1625 1630 1635

Ala Gly Cys Gly Ala Gly Gly Thr Gly Cys Cys Cys Thr Thr Thr

1640 1645 1650

Cys Cys Thr Ala Gly Ala Cys Cys Thr Thr Gly Cys Cys Gly Gly

1655 1660 1665

Ala Cys Cys Ala Ala Gly Ala Ala Cys Gly Ala Gly Gly Gly Cys

1670 1675 1680

Ala Ala Cys Ala Ala Thr Cys Ala Cys Thr Gly Gly Cys Cys Cys

1685 1690 1695

Ala Gly Ala Gly Thr Gly Gly Ala Ala Ala Thr Gly Cys Cys Thr

1700 1705 1710

Ala Cys Cys Gly Gly Cys Thr Gly Gly Cys Thr Gly Cys Thr Cys

1715 1720 1725

Gly Thr Gly Gly Gly Cys Thr Ala Cys Ala Ala Cys Ala Cys Cys

1730 1735 1740

Ala Gly Ala Ala Ala Cys Ala Cys Ala Gly Cys Cys Cys Ala Gly

1745 1750 1755

314

Ala Ala Thr Cys Cys Thr Cys Gly Gly Cys Thr Gly Cys Ala Gly

1760 1765 1770

Cys Ala Gly Cys Ala Thr Cys Thr Gly Ala Ala Ala Cys Ala Cys

1775 1780 1785

Thr Gly Gly Cys Ala Gly Cys Ala Gly Thr Gly Cys Ala Cys Ala

1790 1795 1800

Cys Gly Gly Gly Cys Cys Ala Gly Ala Thr Gly Gly Thr Gly Thr

1805 1810 1815

Cys Ala Ala Gly Ala Gly Cys Cys Thr Thr Gly Thr Cys Ala Gly

1820 1825 1830

Ala Cys Cys Cys Thr Gly Ala Cys Cys Thr Thr Cys Thr Thr Cys

1835 1840 1845

Cys Ala Cys Thr Thr Cys Thr Thr Cys Thr Thr Cys Thr Thr Thr

1850 1855 1860

Thr Thr Cys Ala Ala Ala Ala Cys Gly Gly Thr Gly Thr Cys Cys

1865 1870 1875

Cys Thr Gly Thr Gly Thr Thr Gly Thr Cys Cys Cys Gly Gly Cys

1880 1885 1890

Thr Gly Gly Thr Cys Cys Ala Ala Ala Gly Gly Ala Cys Ala Ala

1895 1900 1905

Cys Cys Thr Cys Cys Ala Cys Cys Thr Cys Cys Thr Gly Gly Cys

1910 1915 1920

Cys Thr Gly Ala Ala Gly Cys Cys Thr Thr Cys Thr Ala Gly Cys

1925 1930 1935

Cys Thr Gly Gly Cys Thr Thr Cys Thr Cys Ala Gly Gly Thr Gly

1940 1945 1950

Gly Cys Cys Ala Gly Ala Ala Cys Cys Ala Cys Ala Gly Gly Cys

1955 1960 1965

Gly Thr Gly Thr Ala Cys Cys Ala Gly Cys Ala Cys Gly Thr Gly

1970 1975 1980

Thr Gly Gly Cys Thr Gly Ala Thr Cys Thr Gly Cys Thr Thr Cys

1985 1990 1995

Gly Thr Gly Thr Thr Cys Thr Thr Cys Gly Thr Gly Thr Gly Cys

2000 2005 2010

Gly Thr Gly Gly Ala Ala Ala Cys Cys Gly Gly Cys Thr Thr Cys

2015 2020 2025

315

Thr Gly Thr Cys Ala Cys Gly Thr Gly Gly Cys Ala Cys Ala Ala

2030 2035 2040

Gly Thr Gly Gly Gly Cys Cys Thr Cys Gly Ala Gly Ala Gly Ala

2045 2050 2055

Cys Thr Gly Gly Thr Thr Thr Cys Thr Cys Thr Gly Ala Gly Cys

2060 2065 2070

Gly Gly Cys Ala Thr Cys Ala Cys Cys Gly Ala Cys Gly Thr Gly

2075 2080 2085

Ala Ala Cys Cys Ala Thr Thr Gly Gly Gly Cys Cys Cys Ala Gly

2090 2095 2100

Cys Cys Thr Ala Ala Gly Cys Cys Ala Thr Cys Thr Cys Cys Thr

2105 2110 2115

Ala Gly Cys Ala Ala Ala Gly Gly Cys Ala Cys Cys Gly Gly Cys

2120 2125 2130

Ala Thr Gly Ala Ala Cys Ala Gly Ala Cys Cys Cys Ala Thr Cys

2135 2140 2145

Gly Gly Cys Cys Thr Gly Ala Gly Ala Gly Thr Thr Gly Gly Cys

2150 2155 2160

Ala Ala Ala Gly Gly Cys Gly Thr Gly Thr Gly Gly Gly Cys Cys

2165 2170 2175

Ala Gly Ala Cys Thr Gly Cys Thr Cys Ala Ala Gly Ala Ala Gly

2180 2185 2190

Ala Ala Gly Cys Cys Thr Ala Gly Ala Gly Ala Cys Ala Thr Cys

2195 2200 2205

Thr Gly Thr Cys Gly Gly Ala Thr Cys Thr Cys Cys Ala Ala Cys

2210 2215 2220

Ala Gly Ala Ala Ala Gly Ala Cys Cys Cys Gly Gly Ala Ala Gly

2225 2230 2235

Gly Cys Cys Gly Cys Cys Ala Gly Ala Gly Ala Gly Ala Ala Gly

2240 2245 2250

Cys Ala Cys Cys Gly Cys Ala Ala Ala Ala Cys Ala Gly Ala Thr

2255 2260 2265

Ala Gly Ala Gly Cys Cys Ala Gly Cys Gly Thr Gly Thr Cys Cys

2270 2275 2280

Thr Thr Cys Thr Gly Gly Ala Cys Thr Cys Thr Gly Cys Thr Gly

2285 2290 2295

Cys Cys Thr Ala Cys Ala Ala Cys Ala Gly Gly Cys Gly Thr Gly

316

2300 2305 2310

Cys Gly Gly Ala Cys Ala Ala Gly Ala Gly Gly Ala Ala Gly Cys

2315 2320 2325

Cys Cys Thr Cys Thr Gly Ala Gly Ala Cys Thr Gly Ala Cys Thr

2330 2335 2340

Cys Thr Gly Thr Gly Gly Cys Cys Thr Cys Cys Thr Ala Gly Ala

2345 2350 2355

Thr Gly Cys Ala Gly Ala Thr Gly Cys Ala Gly Ala Cys Gly Cys

2360 2365 2370

Gly Ala Gly Thr Ala Cys Ala Gly Ala Gly Thr Gly Ala Cys Cys

2375 2380 2385

Ala Thr Gly Ala Ala Cys Ala Thr Cys Cys Thr Gly Ala Ala Cys

2390 2395 2400

Ala Gly Cys Cys Thr Gly Cys Cys Ala Ala Gly Cys Ala Gly Cys

2405 2410 2415

Ala Thr Gly Gly Ala Ala Ala Thr Cys Gly Cys Cys Gly Thr Gly

2420 2425 2430

Cys Thr Gly Cys Ala Ala Gly Ala Ala Ala Cys Cys Gly Cys Cys

2435 2440 2445

Ala Ala Gly Ala Ala Ala Ala Gly Cys Cys Cys Thr Ala Cys Cys

2450 2455 2460

Ala Gly Cys Thr Thr Cys Thr Gly Cys Thr Gly Cys Ala Ala Cys

2465 2470 2475

Cys Thr Gly Gly Ala Cys Cys Thr Gly Cys Ala Gly Cys Ala Thr

2480 2485 2490

Cys Ala Gly Cys Gly Cys Ala Gly Ala Cys Gly Cys Ala Ala Ala

2495 2500 2505

Ala Ala Gly Ala Ala Gly Ala Ala Ala Gly Ala Cys Ala Ala Gly

2510 2515 2520

Gly Thr Cys Gly Ala Gly Ala Thr Cys Cys Gly Gly Ala Ala Thr

2525 2530 2535

Cys Gly Gly Ala Gly Cys Cys Cys Cys Ala Ala Gly Gly Cys Cys

2540 2545 2550

Ala Ala Thr Ala Gly Cys Ala Cys Ala Gly Ala Gly Thr Gly Thr

2555 2560 2565

Cys Ala Gly Gly Gly Cys Gly Ala Gly Cys Ala Cys Ala Gly Cys

2570 2575 2580

317

Ala Gly Ala Gly Ala Gly Cys Ala Gly Ala Ala Thr Cys Thr Gly

2585 2590 2595

Cys Thr Gly Ala Ala Thr Cys Thr Gly Gly Thr Gly Cys Ala Gly

2600 2605 2610

Cys Thr Gly Ala Ala Thr Cys Cys Thr Cys Ala Cys Ala Gly Cys

2615 2620 2625

Cys Cys Thr Cys Ala Cys Cys Ala Cys Cys Gly Gly Ala Ala Gly

2630 2635 2640

Gly Ala Cys Gly Ala Ala Gly Ala Gly Gly Ala Cys Cys Ala Cys

2645 2650 2655

Cys Ala Gly Ala Ala Gly Ala Gly Ala Cys Ala Cys Cys Ala Cys

2660 2665 2670

Cys Ala Cys Cys Ala Cys Ala Ala Thr Cys Ala Ala Ala Ala Gly

2675 2680 2685

Ala Ala Gly Ala Thr Gly Ala Gly Cys Gly Thr Cys Cys Cys Ala

2690 2695 2700

Cys Thr Gly Thr Thr Cys Thr Thr Cys Cys Ala Cys Gly Gly Cys

2705 2710 2715

Gly Ala Cys Cys Thr Gly Thr Gly Thr Ala Gly Ala Gly Ala Gly

2720 2725 2730

Ala Gly Cys Ala Cys Cys Cys Thr Gly Gly Gly Cys Ala Ala Thr

2735 2740 2745

Thr Thr Cys Thr Gly Gly Ala Thr Gly Ala Ala Gly Cys Thr Gly

2750 2755 2760

Cys Thr Cys Cys Ala Gly Cys Thr Gly Cys Cys Thr Cys Ala Gly

2765 2770 2775

Ala Gly Gly Cys Cys Thr Cys Cys Ala Cys Thr Gly Cys Ala Ala

2780 2785 2790

Cys Cys Ala Gly Thr Gly Ala Cys Cys Gly Gly Ala Ala Thr Gly

2795 2800 2805

Gly Ala Ala Ala Thr Cys Cys Thr Gly Cys Thr Thr Thr Gly Gly

2810 2815 2820

Ala Thr Thr Cys Thr Gly Cys Thr Gly Ala Ala Gly Ala Thr Gly

2825 2830 2835

Gly Ala Ala Ala Thr Thr Cys Ala Gly Ala Thr Cys Thr Thr Cys

2840 2845 2850

318

Ala Ala Gly Ala Thr Thr Thr Gly Cys Cys Thr Gly Gly Gly Cys

2855 2860 2865

Gly Ala Gly Ala Thr Gly Cys Ala Gly Cys Thr Cys Thr Gly Cys

2870 2875 2880

Ala Cys Cys Thr Cys Thr Cys Gly Gly Ala Thr Thr Cys Thr Gly

2885 2890 2895

Ala Ala Cys Cys Thr Gly Cys Ala Ala Gly Thr Gly Cys Thr Gly

2900 2905 2910

Ala Ala Ala Ala Ala Gly Ala Thr Cys Cys Thr Gly Cys Gly Gly

2915 2920 2925

Ala Gly Cys Thr Thr Thr Ala Thr Gly Ala Ala Gly Cys Thr Gly

2930 2935 2940

Thr Ala Cys Cys Ala Cys Ala Gly Cys Cys Thr Cys Gly Thr Gly

2945 2950 2955

Ala Thr Gly Thr Gly Cys Cys Thr Gly Cys Gly Gly Cys Thr Gly

2960 2965 2970

Ala Gly Gly Ala Cys Cys Ala Ala Gly Cys Thr Gly Gly Ala Ala

2975 2980 2985

Ala Ala Gly Gly Cys Thr Cys Thr Gly Ala Gly Cys Gly Cys Cys

2990 2995 3000

Cys Thr Gly Thr Thr Cys Ala Thr Cys Thr Gly Gly Cys Cys Thr

3005 3010 3015

Cys Ala Gly Cys Ala Cys Thr Cys Cys Thr Ala Cys Ala Ala Gly

3020 3025 3030

Ala Thr Cys Ala Cys Ala Cys Ala Cys Cys Cys Ala Thr Thr Cys

3035 3040 3045

Gly Cys Cys Ala Gly Cys Cys Ala Gly Cys Cys Ala Cys Thr Gly

3050 3055 3060

Gly Gly Ala Cys Ala Ala Gly Ala Gly Ala Thr Gly Cys Cys Cys

3065 3070 3075

Ala Ala Cys Ala Ala Cys Ala Cys Cys Cys Ala Gly Ala Thr Cys

3080 3085 3090

Cys Gly Gly Cys Thr Gly Cys Thr Gly Ala Ala Gly Cys Cys Cys

3095 3100 3105

Gly Ala Cys Ala Ala Gly Ala Ala Gly Ala Ala Gly Ala Ala Ala

3110 3115 3120

319

Ala Gly Cys Ala Cys Cys Gly Ala Ala Ala Ala Gly Ala Cys Cys

3125 3130 3135

Ala Ala Ala Gly Gly Cys Cys Ala Gly Gly Cys Thr Thr Thr Cys

3140 3145 3150

Gly Gly Cys Ala Gly Cys Thr Thr Cys Thr Cys Cys Gly Cys Cys

3155 3160 3165

Gly Ala Thr Thr Thr Thr Thr Thr Cys Ala Gly Cys Cys Cys Cys Thr

3170 3175 3180

Cys Ala Ala Gly Gly Ala Cys Ala Gly Gly Cys Thr Thr Thr Thr

3185 3190 3195

Gly Gly Cys Thr Cys Cys Cys Thr Gly Thr Gly Thr Cys Ala Gly

3200 3205 3210

Cys Thr Gly Gly Thr Gly Thr Cys Cys Gly Cys Cys Gly Ala Cys

3215 3220 3225

Thr Thr Thr Ala Gly Cys Gly Cys Cys Cys Cys Ala Cys Ala Ala

3230 3235 3240

Gly Thr Gly Ala Cys Cys Ala Gly Ala Cys Thr Gly Cys Gly Gly

3245 3250 3255

Ala Gly Cys Cys Thr Gly Ala Ala Cys Cys Ala Thr Cys Thr Gly

3260 3265 3270

Cys Thr Gly Ala Thr Thr Thr Gly Gly Cys Thr Gly Ala Ala Gly

3275 3280 3285

Ala Ala Thr Thr Gly Gly Ala Ala Gly Cys Gly Gly Thr Thr Cys

3290 3295 3300

Ala Ala Gly Cys Thr Cys Ala Ala Ala Ala Ala Gly Ala Ala Ala

3305 3310 3315

Thr Ala Thr Cys Ala Gly Gly Cys Cys Cys Cys Thr Cys Thr Gly

3320 3325 3330

Cys Gly Gly Cys Thr Gly Thr Gly Gly Thr Cys Thr Thr Gly Gly

3335 3340 3345

Thr Gly Cys Gly Gly Ala Ala Cys Ala Ala Gly Cys Cys Ala Gly

3350 3355 3360

Ala Cys Cys Thr Ala Cys Thr Thr Cys Gly Gly Cys Ala Gly Cys

3365 3370 3375

Ala Thr Cys Ala Thr Cys Thr Gly Thr Ala Thr Cys Ala Ala Gly

3380 3385 3390

Ala Ala Thr Ala Thr Gly Thr Thr Cys Cys Thr Gly Cys Ala Gly

320

3395 3400 3405

Cys Gly Gly Ala Ala Gly Gly Gly Thr Thr Thr Cys Cys Thr Gly

3410 3415 3420

Ala Ala Cys Cys Thr Gly Ala Gly Cys Cys Ala Cys Gly Thr Gly

3425 3430 3435

Gly Ala Ala Ala Ala Gly Thr Thr Cys Gly Ala Gly Cys Thr Thr

3440 3445 3450

Ala Ala Gly Ala Ala Gly Ala Ala Thr Cys Thr Thr Cys Thr Gly

3455 3460 3465

Cys Thr Cys Cys Cys Cys Gly Thr Cys Gly Thr Gly Ala Thr Cys

3470 3475 3480

Ala Ala Cys Cys Thr Gly Ala Ala Gly Ala Gly Ala Cys Thr Gly

3485 3490 3495

Cys Ala Gly Ala Ala Ala Thr Thr Cys Ala Thr Gly Cys Thr Gly

3500 3505 3510

Ala Ala Cys Cys Ala Gly Ala Ala Gly Thr Gly Cys Cys Thr Gly

3515 3520 3525

Gly Cys Thr Cys Cys Ala Thr Thr Thr Cys Cys Thr Gly Cys Cys

3530 3535 3540

Thr Gly Cys Ala Cys Cys Ala Gly Cys Ala Ala Ala Gly Cys Cys

3545 3550 3555

Thr Gly Gly Ala Cys Ala Cys Cys Cys Ala Gly Ala Ala Ala Ala

3560 3565 3570

Cys Thr Cys Gly Thr Gly Gly Gly Cys Ala Gly Ala Gly Cys Cys

3575 3580 3585

Gly Thr Gly Cys Gly Gly Ala Gly Ala Ala Ala Gly Gly Gly Cys

3590 3595 3600

Ala Thr Thr Cys Thr Gly Gly Cys Cys Ala Thr Gly Ala Cys Ala

3605 3610 3615

Cys Thr Gly Ala Cys Cys Cys Thr Gly Ala Ala Cys Gly Gly Ala

3620 3625 3630

Ala Thr Cys Gly Ala Gly Gly Cys Cys Cys Thr Gly Ala Cys Ala

3635 3640 3645

Gly Cys Cys Cys Thr Gly Thr Thr Thr Ala Cys Cys Thr Gly Thr

3650 3655 3660

Ala Gly Ala Ala Cys Cys Gly Cys Cys Thr Cys Cys Ala Thr Cys

3665 3670 3675

321

Ala Gly Cys Gly Thr Gly Ala Ala Cys Ala Thr Cys Ala Gly Cys

3680 3685 3690

Ala Cys Cys Cys Thr Gly Ala Thr Gly Ala Gly Cys Ala Thr Cys

3695 3700 3705

Cys Thr Gly Gly Ala Ala Gly Cys Cys Ala Thr Cys Ala Thr Cys

3710 3715 3720

Ala Ala Gly Ala Ala Gly Ala Thr Gly Ala Ala Gly Ala Ala Ala

3725 3730 3735

Cys Gly Gly Ala Thr Cys Ala Thr Gly Gly Thr Gly Cys Ala Cys

3740 3745 3750

Cys Gly Gly Thr Gly Gly Ala Thr Cys Thr Thr Cys Thr Cys Cys

3755 3760 3765

Ala Ala Thr Cys Ala Gly Cys Thr Gly Ala Cys Cys Gly Thr Gly

3770 3775 3780

Cys Ala Gly Ala Thr Gly Cys Thr Cys Thr Gly Cys Cys Thr Gly

3785 3790 3795

Cys Cys Thr Cys Thr Gly Cys Thr Gly Ala Cys Cys Cys Cys Thr

3800 3805 3810

Gly Ala Cys Ala Cys Cys Ala Gly Ala Thr Gly Gly Ala Thr Cys

3815 3820 3825

Thr Thr Thr Ala Gly Thr Ala Ala Thr Cys Ala Gly Cys Thr Gly

3830 3835 3840

Cys Ala Gly Ala Cys Ala Gly Thr Gly Cys Ala Ala Ala Thr Gly

3845 3850 3855

Cys Thr Gly Thr Gly Thr Cys Thr Gly Cys Cys Ala Cys Cys Thr

3860 3865 3870

Cys Thr Gly Ala Ala Gly Cys Ala Gly Gly Gly Cys Gly Ala Gly

3875 3880 3885

Ala Gly Ala Ala Thr Cys Ala Thr Thr Thr Gly Gly Cys Cys Ala

3890 3895 3900

Cys Thr Cys Gly Gly Cys Gly Gly Cys Thr Thr Thr Cys Ala Gly

3905 3910 3915

Ala Thr Cys Cys Cys Cys Ala Thr Thr Thr Cys Thr Thr Cys

3920 3925 3930

Thr Gly Gly Thr Cys Cys Cys Thr Cys Gly Gly Cys Ala Thr Cys

3935 3940 3945

322

Gly Gly Ala Cys Ala Cys Ala Cys Thr Gly Gly Cys Gly Cys Ala

3950 3955 3960

Thr Gly Gly Thr Gly Gly Cys Ala Thr Ala Cys Thr Gly Gly Thr

3965 3970 3975

Ala Gly Ala Ala Gly Gly Cys Gly Gly Ala Gly Ala Gly Ala Gly

3980 3985 3990

Thr Gly Gly Gly Gly Cys Gly Gly Cys Thr Cys Thr Ala Cys Cys

3995 4000 4005

Gly Thr Gly Thr Cys Thr Ala Gly Ala Ala Gly Cys Cys Cys Ala

4010 4015 4020

Cys Thr Gly Thr Gly Gly Ala Thr Cys Ala Gly Cys Thr Cys Cys

4025 4030 4035

Ala Thr Gly Ala Thr Cys Thr Ala Cys Cys Thr Gly Ala Gly Ala

4040 4045 4050

Gly Gly Cys Ala Thr Gly Gly Cys Cys Thr Thr Cys Gly Cys Cys

4055 4060 4065

Ala Gly Cys Gly Ala Cys Ala Gly Cys Thr Cys Cys Ala Thr Cys

4070 4075 4080

Ala Gly Ala Ala Cys Cys Ala Cys Cys Ala Gly Ala Gly Thr Gly

4085 4090 4095

Thr Cys Cys Thr Gly Gly Thr Gly Thr Cys Gly Gly Ala Gly Ala

4100 4105 4110

Thr Gly Thr Gly Gly Cys Gly Cys Cys Ala Ala Gly Thr Cys Thr

4115 4120 4125

Gly Cys Cys Gly Cys Ala Gly Cys Cys Thr Cys Thr Thr Cys Thr

4130 4135 4140

Thr Cys Thr Cys Cys Ala Cys Thr Gly Thr Gly Gly Gly Ala Thr

4145 4150 4155

Thr Gly Cys Ala Gly Cys Ala Cys Cys Ala Cys Ala Ala Cys Cys

4160 4165 4170

Gly Gly Cys Ala Cys Ala Cys Ala Cys Cys Ala Cys Gly Gly Ala

4175 4180 4185

Thr Gly Cys Cys Thr Gly Thr Gly Gly Gly Ala Gly Ala Thr Cys

4190 4195 4200

Cys Cys Thr Ala Gly Cys Gly Thr Thr Ala Cys Cys Cys Thr Gly

4205 4210 4215

323

Cys Thr Gly Gly Cys Thr Ala Ala Cys Cys Thr Gly Cys Ala Cys

4220 4225 4230

Cys Thr Gly Thr Gly Cys Thr Ala Cys Thr Ala Cys Cys Ala Cys

4235 4240 4245

Thr Cys Thr Cys Ala Gly Ala Gly Cys Ala Ala Cys Ala Gly Ala

4250 4255 4260

Ala Ala Cys Ala Ala Gly Ala Gly Cys Cys Gly Cys Cys Ala Gly

4265 4270 4275

Ala Thr Gly Gly Ala Ala Thr Cys Cys Cys Thr Gly Gly Gly Cys

4280 4285 4290

Ala Thr Gly Ala Ala Gly Cys Thr Cys Cys Ala Ala Cys Thr Thr

4295 4300 4305

Ala Ala Ala Ala Ala Gly Ala Ala Ala Gly Thr Gly Ala Ala Cys

4310 4315 4320

Gly Cys Cys Thr Gly Cys Ala Gly Cys Ala Cys Cys Thr Ala Cys

4325 4330 4335

Ala Cys Ala Cys Cys Cys Cys Ala Cys Ala Thr Cys Thr Gly Cys

4340 4345 4350

Ala Gly Ala Cys Ala Cys Ala Cys Cys Thr Gly Thr Cys Gly Cys

4355 4360 4365

Gly Thr Gly Ala Ala Cys Gly Cys Cys Gly Thr Cys Gly Ala Gly

4370 4375 4380

Gly Ala Ala Cys Thr Gly Cys Ala Cys Thr Cys Thr Ala Gly Ala

4385 4390 4395

Ala Gly Gly Ala Thr Gly Thr Cys Cys Gly Thr Gly Cys Gly Gly

4400 4405 4410

Ala Thr Cys Gly Thr Gly Ala Ala Ala Ala Ala Thr Thr Gly Gly

4415 4420 4425

Gly Thr Gly Thr Cys Cys Thr Gly Cys Thr Gly Cys Thr Ala Cys

4430 4435 4440

Gly Thr Gly Cys Gly Gly Cys Thr Gly Thr Cys Ala Gly Cys Thr

4445 4450 4455

Gly Thr Gly Gly Gly Ala Cys Thr Gly Thr Cys Thr Ala Cys Thr

4460 4465 4470

Thr Gly Gly Ala Gly Cys Gly Cys Thr Cys Thr Gly Gly Ala Cys

4475 4480 4485

Ala Ala Cys Ala Ala Gly Ala Thr Cys Ala Thr Cys Thr Gly Cys

324

4490 4495 4500

Ala Ala Gly Gly Gly Cys Cys Ala Cys Ala Gly Ala Ala Thr Cys

4505 4510 4515

Ala Cys Cys

4520

<210> 1100

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 1B

<400> 1100

accgagatcc aattgaccat gaacgacagc aagcacaagc tggaaagccc cgctctgaaa 60

caggtgtcac cagcctctcc acctactcag cagcctcaga cacctcagga tagcagacag 120

gtgctggcca tgcctagcag ctggtctagc tctggcagaa cccagcggaa ggtggtcaag 180

aatcctagcc agcctgtgcc tctgagaaac gacggcatca ccaacggcaa cgactccacc 240

agaagctgca gcatcagcct gaagaaaagc ctggaaaaga tgcccctcat gatgaagaag 300

aaggacagca ccatcttcat cgtgcccatg agcctggcct gtagcatcac cgtgtctttc 360

gtgggccaga tcagcacact ggtgctggat gtgtccagca tctcccactg gaacaaagtg 420

cggcagaact tcaagcggaa cgccgacaag aagaacgttc ggagcggcga gaagaacgac 480

ctgcagctgt ttgtgatgag cgacaccacc tacaagatct actggaccgt gatcctgctg 540

aacccctgcg gcaatctgca cctgaaaacc accagcctgg gcgactgtcc tagaacaatg 600

gccggacctt ctgcctctct gcaccctgca tctggcagag cctctagaaa gacctggcag 660

agaaggctga gagcctgtag agctggtgga cctgctggat gggccagaca ggccttcgat 720

aagaaaggct ttgtgatcct gagcgtcgga gccggaacag gcggaggacc tgcttgtgtt 780

cctgtggctg aagcttctac aggcgcttgg acagctgaag gcgccgctgg aacaagatgt 840

agagcttcta gagccccttg cagacagctg cctgccagct ctcctaccaa gtgcgagatc 900

tgcgtgaagc gcagacggcg gagaaagaga agcagccagt ctagaagccc cgtgcctaag 960

aaacgcgaga gaatgatgct gcccgtgcgg gccagaacag ctagatctgc acttcctcag 1020

gggagagccc ctcttgaggg aatgctgctt cctagagcac tgcccagcct gctgtctaga 1080

atgccagcca gaagagagag ccggttttgg ctggctctgc aggcacttgg actcagacct 1140

ccacatctgc ctagctgtca tcctctgcct caactgcttg gccctccacc tcctcatcag 1200

gcccctacag ctttcttcct gctggacatc attctgcggc tgccctgcaa agtgaccgag 1260

tctagcaata tgggcggcaa cagatccatc tgcctgacca acgccaagaa aatggaagat 1320

1380

tgttgtctgc ccggactgac acatcctgct caccagccac tgggatctat gaccctggcc 1440

atcagattca tcagcgctcc tcagcctagc tccagcacag tgacagctgc tcctagcggc 1500

cagtacttca agggcaagaa gcggcggaaa gagatcttca agaagaagaa acccctgaca 1560

ttcagcccca gcctggccaa aaagaccctg accaatagca gcttcttcta caccagcgac 1620

cgcgaggcca agaaatctat gaagcggagc atgaccctga aggccctggt caagacctgg 1680

acacagttca gaaactaccc cagagcctcc tctcctggac accctctggg acctaatggc 1740

atctgggaag tgatgcccag aacactggaa ggctgcatct ccgtgaagga ccacagaaca 1800

agccagaacc acagcaagtg catcaccgag tgtgtgcgga cagccctgaa cagaagaagc 1860

aagatcgagg cctctcctat cggctctatc gccctgagat ccatgcctaa gagcagaaac 1920

caggtgctgt tcagacggaa tctgacccca agccagctga gaactctggc ccctccttat 1980

atgctgctgc cccagtctct gagccagagc ctgagcagaa agcagatccc tagccagtcc 2040

atgctggtgg cctctagagc aggacagttt cctgtgtggc ggagaaccag cagaggaatg 2100

agcctgcagt ggaagtccgg caatgcctgc acacctctga gatgccctag cagaagccct 2160

gccacattcc tgacagctct gaacggctgg cacagatctg gactgagagc ccagagaaag 2220

cgcaagcgcg agaagagaag ccagaagtcc aggcagaacc atctgcagct gaagtcctgc 2280

cggcgcaaga tctccaactg gtccaagctg agaagcccct ggatcttcgt gtccaagaag 2340

agactgccca agctgaccaa gtgtgaaatc tgtgtcaaga acaccgccat cctggctatc 2400

accctgatca ccctcagga ccctcatctg gaccctgtgt gtcaccctca tagaaggcct 2460

ggatggaagg gccaccacag agtggtgttt tctcatcacc ctagcggcag caccaagagc 2520

cactggcagc aaagagaacg cggaggcggc agacgggaaa agaagggctg tagagagaga 2580

tccctgctgt actccagaat gtgcctggtg cagatcccca cacacacctg ggagcccgat 2640

tacttcgtgt ctctgcccat gatgttcagc tgggccacac agtcattccc cgtgggagcc 2700

gtgtttctga ccagacctgc cattccttgc tgtagctgca caagccacag caacccttgg 2760

agtcagagca gacacccaca cagaagccca cctacctctt gtcctccgct gctggaagcc 2820

tttccactgc ctagagcatc cctgagagcc accgtgccaa gacatagctc tgtgctggca 2880

accggactgc atctgcacag acagatttct ctgcccctga gaggattcct gctgcatcaa 2940

ccactgccag gccagcatct gtggctgtct catagactgc ggctgagact gccaagaact 3000

cagcggcctc ctagatccat cctgagcctt agactgccac tgaggccaca gagaggaccc 3060

agacctctgc atagatggct gcagcagacc acaggctgta gccagagaag ataccccaga 3120

ctgaaaacct tcggcgtgcc actgggctcc attctgtgtc tggctggatc tctgagcacc 3180

atgatgatca ttagcaccac cagacaagag tgtggcaggc gggcatctgt gccctgtaga 3240

cctatgatcg gaagcgccag accaggacct tggagaacat ctgctatgcc tagcgccatg 3300

ggcgtcgcac tgcctacaag ctgtgaatcc ggaagatctc ctcctgccac cggcggaaga 3360

atgcctggaa gccagagcat tatgatgagc tggatgcctc ctctggctcc ttgtgccgct 3420

tgtccttgtt ctcccgctcc tacactgtgt cccgctcatc ctgctagagc acctacagcc 3480

gtgcctgcct ttacacctct gtctgcacat cctgtgcctg tgctgtctgg atgtcacctg 3540

gccgtcagaa cctccatgct gacactgctg cctatgctga agctggcccc actgcaagtg 3600

accccacac tgaagctgat cctggtcatg ctggaaatcg tgaccgagag cactcggaga 3660

gagatgcctt ctcaggccac accttctcca cctggacctt ggcgagccgc tctgggcaaa 3720

3780

ctggccccat acttccccag acggatgacc agacggacca tgacaaccac caaaatgatg 3840

atgatgcggc ggaccaagcg gagaaggcgc aaaaggcgga gaatgacaat gatgacaagg 3900

cggaccctgc tgacaaagaa aacctgtctg acccctagaa ccagcgccgc taccaaagct 3960

ctgccaaggc agacaatctc ccagcgggagg cctattcaga cccctcctgg aacatctctg 4020

gccatctggg gcaaccagca gctgaaatgg acccctcaca tcctgaagtc cgccagcaga 4080

agggccagac ctaccagctg tagcacctgt agaatgtggt ggatcgccaa aaagcggatc 4140

aagctgcggc ggaacagaac cagactgaca ctgacaggca caagaagcag cggcaccaga 4200

gcttctccat ccgccgtttg cctgtgttgc tggacctgtc cttccagaag cttcccagcc 4260

gcttctccaa gcggattgag agcacctgtt ggagcacagg cccctccaga agttacactg 4320

agagctagct gtgcctccag cgcctctaag gcttgtgctg gactttggcc ttggggagca 4380

caagttcggc aaccatcttt gcctggacca tgcgcttctc tgctgagcac actgtctcag 4440

ccacctccac aggctccatc tcaagtgtgg acagccgcaa cactgagatg tcctgctgtt 4500

ccagctgctg cctgtcctcc t 4521

<210> 1101

<211> 4053

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 2B

<400> 1101

agaaagatgc agagcctgaa tcaccccagc agactgacca acatgagcag aacacctcca 60

agacgcgtgt gcaaccctcg gagaaagacc tggaagatgc ggaagaagta cgcccagaag 120

cctagccaga agaaccacct gtacaacagc cggaagaaac tgctcgagga catgaagcag 180

aatggcttca caggccacag agaggggcagc gagtctgata atctgggcct gctgtactcc 240

tttcctccta tccggatgtg cgccctgtct caccctagct tctacatcag cagcaccgtg 300

tgctttctga gcaagcactg catgaccgcc tgcacacaaa agaaaggcca catgatcttc 360

agccgcgaga agatcatcaa ccccaccatc agctgcccct ttcagagccc cacaaagcgg 420

ctggaactga gcctggaacc ttggattccc tacctgcacc agcagaaaac ccaaggcggc 480

aagaagagaa gcaagttccc tggcaccagc ctgcagtgct acctgaacag atgcagcctg 540

atggaacaga tccctcacct gaagtccgcc tctccacaga tcatgcagag aaccagcgcc 600

gaccggttcc ggatgaagcg gagaatgcag aacctggaca atctgggcgt tccatggcca 660

ctgctgcctg gaccctctgga aaacacacag atccccagaa caagccctct gtggaagatc 720

acaatggtgc tgacataccc caactgcgtg gtgcacgtgc tgatgttctg gctgcctatg 780

agatctctgg gcgagccttg gagagctggc tttcagtggg gagagtcccc ttggcaagtg 840

atggctagcc tgacaggccc ctatcctatc agcgagggcc tgaccatgac caccggcaga 900

aacatcaaca gcaactccct gatgaccaac tgcctgttca gccccatctt cctgtgccac 960

cacgccatca tgcaccggaa gatgacaagc ccacacttca gactgttcag cagcaagatc 1020

ccacatcctc aggtgccatc tgtggtggcc ctgtgcaagt tcagagccac atttctgctc 1080

gccctgtggg agtgtagtct gcctcaagcc agactgtgcc tgatcgtgtc tagaaccctg 1140

ctgctggttc agtctgccgc tcctccacat agactgctgg gcccttctag atggtccaga 1200

agccacctgg acttcagccc ctactggatg aagaaagtga agctggccaa cggcctgtgg 1260

ttcaacctga tcttcgtgct gttcaaggcc gaccagagcg agtctagcct gtctagcggc 1320

cggaacaaga aaacaaagct gtggttctcc ctcatcaaca tccaccaccg caagaatcct 1380

1440

ctggcccata tgtacggctt cagcacctac ggcacccaga aaggcgagtg cgtttgtctg 1500

cagtgtctga gcggcagcag cctgttcatt ctgaagcagc ccctggacct gaagatctcc 1560

aagaccttca tgctgcaagc tcacccacag gtgccagctc ttgctcctca agcttggtgg 1620

cctgctagaa gaggccctct gacatctgct ccatccgctc agcagtctct gacagctagc 1680

cctcagatta tgcagcggat gagcgccgtg aacttcgaga caatcatcta ccctccaagc 1740

acctgtagcc tgccttgtag aggcagaaca ctggaaagcg ccggactgtt tccttggctg 1800

cacacccagt gtacacaccc ctgtaccatg ctgctgccac aaggctggtg gtgtacaaga 1860

ggcggcggaa agcggaaaaa gaaaatgtgc aaggtgtccg gcggctgcac cgcctgtgtt 1920

gagagatgtc cttggaaggg catctgctgg aagtggccag tgacagtggc tgctcagtgg 1980

tctagcgtga cctctctgga atggacagcc caagagagag gctgcagcag ctggctgatg 2040

aagcaaacat ggatgaagtc ttggtccctg cgggacccca gctacagatc catcctggaa 2100

2160

aagcagtctc aggtgcagct ggccattcag gtgctgctcg tggacaagca caagtacttt 2220

ctgttcggca gagcccgcaa ccggaacatg aagaaaacca tgagccacct gatcgcccat 2280

caggccctga agatgagcgg cctgaggctg tttcatcctc ggctgctgac aagaaagaag 2340

cccggcaagt tcctgcctag cagatttcct tggggccctc ctcctctgtg tcatcctgct 2400

tggctgagcc ctatctccgc ctgtccaaga ctgctgccat atccttttca cgtgctggcc 2460

ctggaagtga cctttccacc tggaacagcc atgcctcaga tgcaactgca ggccctggcc 2520

atgtgggaga aaggctggga taagcggaag agaatgaaga ccggcgccat ccaagagaag 2580

ggcgctattc aagagaaagg ggccatgacc aagcggatct gcgtggtcaa tttcgaggcc 2640

tgcagagaga tggaaagcct gagcgcccct gagaagatta ccctgtttga agcccatatg 2700

gcccggtgca ccctccatcaa tgtgctgtgt gtcagagcct ctctgatcga gaagctgatg 2760

aaagaaaagc gcaagatgaa gcgcaaccag gtggcatccc cacaaattat gcagaggctg 2820

ttcattaagg gctaccagaa caaccacttc accttccatc tgcaaaactt cgccttcttc 2880

agcagcttcg gcaacaacaa gtacatcaat atcctgacgc ggcgcaagaa aagagaggac 2940

cactgcggac tgctgatcct gatccagcac ggcggcatct tcacctttcg gttctgggtc 3000

gaaaagctga gctggaacat ctgcaagaac ctgagcctgt acctggataa gttcgtgcag 3060

cggctgctgt acgtgtacat catcgagaca aacgtgttcg tgctgaacct gttccacgtg 3120

ccagtgaacc agatcttcat gatggccttc tacctgaagt acaccgagaa ggcctgggtc 3180

atggaactgg aatggctgat ccaacagcag tgttaccagc aagagaagaa aagccggaaa 3240

ctgatgagca gctgccgggc cttcatccac acacctagaa gccctggctg cagcgtgtac 3300

gccaatatgt gctgggcctt ttggaagcct cctagccagg gcatcagctc ttgtctgatg 3360

acaggctgtt ggggcagccc tcctctgcac ctgtgtcagc ctctgaggcg gtataagaaa 3420

aggggcgtga tcgcctggcg gcggagaaag tggcagatcc ggcatgtgct gaaaaacctg 3480

atcctgtttc atgtgcggaa catcgtgtac ggcatccaag tgatgctgtg cggcaaggga 3540

ctgacacacc ctgtgcctct gaaacggacc ctgcagaaca agaggaacaa gaacatcaag 3600

3660

ctgaatagcc ccgagagcaa ccccaagttc ttcctgacct ttagccagct cgagaagaaa 3720

gctacctgga tgagaagaac aggcgccctg agagccacag ctcctgctac agcagctcct 3780

caggctcaga ctcctcattg gagagccgcc acaacaagca gcatgagcag cagccctaga 3840

gccgagccta catcttctag ctcttgggcc acctgttggc ccagatgctg tagctccagc 3900

tgttactggt ccctgagttc ttggcctcct gctggcgccg ctgaggctac aaacaccaga 3960

actagaagca gagagtccca gcggggcaga atgctgactt ggagaagtag tctgtggctg 4020

cagggcacca gatgttttac aggcgtgcgg aca 4053

<210> 1102

<211> 4518

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 3B

<400> 1102

ggagctgctg ctgcctcttg tcagtctcct ccttctcctc gggaaaccac acctagctcc 60

gacgccacca gacggacaag accaattgcc gctcagagca agaaaaccat gacagccgcc 120

ggactgaccc ctaagaacaa gaagcggaga aagctgcggc ccaagatgat ccggaagatc 180

cagaagagaa ggcggaccgc caaccggcgg aatagcagaa gggctacccc tacaagcaag 240

ggcagcctga gaagaaagta cctgagagtg cggggacctc ctctgcacac ccggaacatc 300

agaaagctga tctggaccaa gcagcggagc ttcctgtctc tgttccccaa gctgagagcc 360

accgagcaga tgaccaatgt gggctgctct atccctcacg tgcccaaagc cacagccgct 420

cactctgtga cagtgaaact gcagctgccc ctgaccttca gcaccctgct gtgggatagc 480

agacacacca tgagcctgag caacatcctg atgaagttcc ggctgcagtc tagcccattc 540

ctggccagat cctggaacac catgcagtgg aacaacaaga aaatggccat gaccgtgatc 600

gctggcgccc accactgtca gtacaagctg acctacacca aagtgtggaa gatgaactgg 660

aagaaaatga tgatcggcag ccagcatctg ctgttcagcc ctctggatag cagtcccctg 720

cagctcctgc tgcaccctca cggcaagaga tgcaaccctt gctgcagact gacctggatg 780

agccagaaca ccagaagcac caacctggaa aagaacccca gcctgaagaa gcagagccag 840

cggagaggaa agctgagcgc ctttagcggc agaatcctgc accagccaga aatggccgcc 900

atgagactga tgcccaagtt cctgaactcc accaacagct ggtggaccct gaccacactg 960

agcttcgtgc tgatcgagga agccagacag cccagcagac ctaccggcag aacacctacc 1020

accagactga tggcccctgt gggcagcgtg atgagctgtt ctctgctgac ccagccttct 1080

ccagccagcg cctgtacaat gcctccactg ctgcatccac agtgggcccc tcatagtgct 1140

ggactttctc caggcgcttg cagacctgcc tgcacctgta tcagcatgac caccatcagc 1200

agggccacat ggggcccagt gtactacgtg tgcaaccgga tgaacgtgtc cagcgtgctg 1260

aagcccttcc tgttcaccgt gcctatcgag gacatgagag agtgcaccct gggcagaaac 1320

agaatgaatg tgtcctctgt gctgaaaccc tgtctgatcc ctgtgccaat cagcgagaag 1380

aagaggaagc tgcccaacat cgagatcacc cctcagcggt ctaaggccat gctgcagaag 1440

cagtgctgct acaacctcgt gatcaagcac cctctgatct tcagaatcaa cctgcggcgg 1500

cagggcaaga ccaaagatgg cgttgtgcag agcctgggct tcctcaacca gcagatcatc 1560

ctgcagaaag agttcttcat ctgcctgctc atgttcatca agcggtggaa gcctgccgcc 1620

accaagagca gaaagaaggg ccacaccaag aacagacggga aggccaagag aaagccccag 1680

atctacctgt tcttcctgac cgtgtgcggc tttcacctga aaaagaccag cgccaagtgg 1740

ctgaagaagt ccaccaagtc cagatccaac ggcatggaag cttgcggcac acaccccact 1800

cctagactga gctgcctgag agttagatgc ggcggctggc tgtgtggctg gacacctaga 1860

ggtagaaagt ccggcgtgat gaagtgctgg gatctgtgcc ccgtgcacaa gaagagaggc 1920

ctgttcagac tggattttca ccccagaagc agctccggca agggaaagaa cagcccctgc 1980

tgccaaaaga aactgcgggt gcagaaccag ggccatctgc tgatgattct gctgcacaat 2040

gaccgggtgt tcctcgtgaa gatcggcgag aagctgaagc actgtttcaa ggcctggggc 2100

tccagacgga accggcggta cagaaagatc tacggcgagc tgaagatgtc cctgcaggcc 2160

ttcctgatcc acctgagaaa ggtggacacc caccacctcc aggtggtcac caaaacagtg 2220

accgccacaa cagccgacgg cggagaatg cggagactga agtgcaaggc ctgccggctg 2280

agatgggcca gacctgaacc tgctgctcag gcccctccta agagccactt tccacctcat 2340

cctagcggcc ccagcagaac aagtagcctg cagctgagaa gcctgttctt tagccactgc 2400

ctgtgcagcc agaagaagga ccctgccggc aagctgaaaa agctgctgaa gaaaagcagc 2460

cagctgcgga gaagctggcg caatgtgtcc agaatcagca cccctcctcc actgatcctg 2520

tggcctcaag ctgctcctgt gtccgtgatc accatgaagc tgtgggagaa gcacttcaag 2580

ggcctgaatt ggaacctggt cgtgtggatt ctgaacctga agatgatgac ctacatggaa 2640

atgcactacg gccatcaggc cccacaggtg gcaatgctgc ctaagctgct gtgcagacag 2700

atgctgccca ctctgagcac actgcagcag agacatcctc agggcccttg tcctcctcct 2760

gtgacacctc tgagagtcca gtctgtcctg ctgctgggag tgcctcagac agccagagga 2820

cctagaagaa aggtcctgat ggtgctgtgg ctgcccgcca atcagtctct gcagaaaagc 2880

caggttttcc agtgggtgct gcggacagag gtggccccta gatgtcctag atctgccgtg 2940

gacatcagcc ccggacctac atctcctagc ctggcctctt gtgcctctgt ggccattgga 3000

aagctggcac tgcttggcgc ctctgctcct tgtggtgctg ctggcgctag agaggccgga 3060

agatttgctg tgctgggcac catggtcatg aagctttgtt tcttctacct gctgtaccgg 3120

ctgcgcatcc caagcctgag gaagtacaga cagcacctga accggaagct gcacctgaca 3180

agatcctgcc tgatcgatct gaccatgatg atgtcccaga agctctggaa gaaccagcgg 3240

aagagaatcc ctctgccatc tccacggcaa catctgctgc cacagtaccc tctgcaccca 3300

cctccacctt gtctgctgtt gctgcaccat agacaccacc tggcctttct cgagatggcc 3360

tgtagctctc agcacaccca cagcatcaag atctggatca gcttcagcca tgaagtggac 3420

cctccaggca gcgtgtggat actgccttgc gatctgcctc ggatgctgcc aatctcttca 3480

cccctgcaca gaccccagat gcctttctgg acctgcacct ttcctgccac aacctggggc 3540

acatgggaga caccttctac atggggctgg cggactttca gaggcgctag cagcagcaca 3600

tcccgggaca gcagatcctt cttcacctct gctcacccta gcaggccctg cagaatgaga 3660

ctgtaccagt gcaagaagaa gctcctcctg aaactgctga ccaccaccac aagcagcccc 3720

agaagatccc ctgctctgag agcaagaggc ggcaccacaa tcggcgaagt gaccagctgt 3780

accaacctga gcgtgcccat gatgctgacc atcctgattt ggaagcgggt gttcatcctc 3840

ctgctgagcg ataagaaggg cgccaagaag atcaccttcc tgcctattcc tccacacctg 3900

agacaccctc tgctgctgtt cgagaagaaa cggcctgagt ggctgagcat ggacctggac 3960

ggaatgctga cagtgatcgg ccagggactc gtgggcatga cccctagaga tctgggaaca 4020

gtggcaggcg ccaccgctca gattcagcga cctatccagg tgcagactgg cggcagactc 4080

agaagaaacc tgcagagaaa cctgaaggtg ctccaggtgt ccgtgtacag cggcaacctg 4140

ctgtccttta gcagacgggg catgaagtct agcgtgcggc tgaaaaaccg cgagtgggag 4200

aaacggaagc agattctcgg aggcggcgga aagtacaaag agtacttcct gaagcgcatc 4260

ctcatccgga aggctatgac agtgctggcc ggcgacaaga aaggactgggg cagattcatg 4320

agatgcgtgc agagcgagac aaaggccgtg tctctgcaac tgcctctggg aagaaacatg 4380

gaacaccggt tgaccatgga cagcgccctg agcctgacaa agagagagggg acccgagaca 4440

ctcaaaaaga aatacctggt ccagcaccag atgaagtgtc tgaccgccgt ggattgtctg 4500

caggcttttt gggacatg 4518

<210> 1103

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 4B

<400> 1103

atccagaaga agaagcagaa gtccaacctg gacctgcagc accagcgcag acgcaaaaag 60

aagaaggaca aggtcgagat cgggaatcgg agccccaagg ccaatagcac agagtgtcag 120

ggcgagcaca gcagagagca gaacctgctg aatctggtgc agctgaaccc tcacagccct 180

caccacagaa aggacgaaga ggaccaccag aagcggcacc accaccacaa tcaaaagaaa 240

atgagcgtga tggccaagaa gtcccagcag cctctcggac aggcctttgg ctctctgtgt 300

cagctggtgt ccgccgattt cagcgcccct caagtgacca gactgcggag cctgaaccat 360

ctgctgatca acgaggggcaa caaccactgg cctagagtgg aaatgcctac cggttggctg 420

ctcgtgggct acaacaccag aaacacagcc cagaatcctc ggctgcagca gcacctgaaa 480

cactggcagc agtgcaccag agccagatgg tgccaagagc cttgtcagac cctgaccttc 540

ttccatttct tcttcttttt caaaacggtg tccctgtgct gccccggctg gtctaaagga 600

caacctcctc cacctggcct gaagccttct agcctggctt ctcaggtggc cagaaccaca 660

ggcgtgtacc agcacgtgtg gctgatctgc ttcgtgttct tcgtgtgcgt ggaaaccggc 720

ttctgtcacg tggcccaagt gggactcgag agactggtgt ctctgagcgg catcaccgac 780

gtgaaccatt gggcccagcc taagccatct cctagcaaag gcaccggcat gaacagaccc 840

atcggcctga gagttggcaa aggcgtgtgg gccagactga gagcctccgt gtctttctgg 900

accctgctgc ctacaacagg cgtgcggaca aggaagcc ctctgagact gacactgtgg 960

cctccacgga acatcctgaa cagcctgcct agcagcatgg aaatcgccgt gctgcaagag 1020

1080

1140

aagctgcaga gccctctgtg ggattgctcc accacaacag gcacacacca cggctgtctg 1200

tgggagatcc ctagcgttac actgctggcc tgcagagtga acgccgtgga agaactgcac 1260

agcaggcgga tgtctgtgcg gatcgtgaag aactgggtgt cctgctgtta cgtgcgcctg 1320

tctgctgtgg gcctgtctac ttggagcgcc ctggacgtgg tcatcaacct gaagcggctg 1380

cagaagttca gccagaagtc tcagcaccac tgcgccctga gaaagatcga gtgggccaca 1440

aagctgagcc tcggcggaaa agctggaccc caggctcaag agaaggccaa cgccaagaag 1500

atgcccttcc agcggtggat cttcagcaat cagctgaccg tgcagatgct gtgcctgcct 1560

attgaagccc tgactgccct gttcacctgt cggacagcca gcatcagcgt gaacatcagc 1620

accctgatga gcatcctgga agcccctctg ttttttcacg gcgacctgtg cgaggaaaag 1680

aagaacggcc ggatcagcag ccaggctcct ctcagacttt ggtcttggtg cggcacaagc 1740

cagacctact ttggcagcac cgccaagaaa agccccacca gcttttgctg cgagtgggga 1800

ggacaggctc accggcagag aaataccgga atggaaatcc tgctgtggat tctgctcaag 1860

atggaaattc agatcttcaa gattgcctg ggcgagatgc agctgtgcac ctacacaccc 1920

cacatctgca gacacacctg tagaggcgga cctagcgcca gcgatagatg ctgtagctgt 1980

agccctagcg gcttcagcgc cggaagaggc agatgtccag ttcagggatg tctgcggcct 2040

cacagagtgc agctgctgag aagatggggc cctggatctc ctgctggcca gagactgtct 2100

aagggctttc agctgctccg ttggtgggga ccaggatctc cagctcctga gcctagaaag 2160

ggcccatttc caccacctga tcctccatgg cctgtgaccg ctgtgacagt gatggctggc 2220

tctgtgccta gcgctcagtc tgtggatgct ctggaaagcc ctggacctct ggcactggaa 2280

ggacctagca gccccagaaa cctgctctgg cgggaaatga gcatcttcct gcctggcatc 2340

ttcacctctc ggattctgaa cctgcaagtg ctgaagaaga tcctgcggag ctttatgaag 2400

ctgtaccact ctctggtcat gtgcctgcgg ctgcggacca agctggaaaa agctctgagc 2460

gctctgttca tctggcccca gcacagctac aagcagaggc ctcctctgca gcctgtgcag 2520

gtcgtgctca gactgggaaa cctgcctcca ttcttcagag gcagcgaggt gccctttcct 2580

agaccttgcc ggaccaagat gctgaaccag aagtgtctgg cccctttcat cacacaccca 2640

ttcgcctctc agcccctggg acaagagatg cccaacaaca cccagatcag actgctgaag 2700

cccctgctga cccctgacac cagatggatc ttctccaacc agctgcagac agtccagatg 2760

ctctgtctgc cacctctgaa gcagggcgag agaatcattt ggcccctcgg cggctttcag 2820

atccccaact tcttttggag cctcggaatc ggccacactg gcgcctggtg gcatactgga 2880

aggcggagaa gagaatgggg cggctccacc gtgtctagaa gcccactgtg gatcagctcc 2940

atgatctacc tgagaggcat ggccttcgcc agcgacagct ccatcagaac caccagagtg 3000

3060

cgggacatct gtcggatcag cgactctaag aagatgaccc agaagcgcga gagcaccctg 3120

ggcaatttct ggatgaagct cctccagctg ccagacaaga agaagaagtc taccgaaaag 3180

accaagatca tcaagaagat gaagaaacgg atcatggtcc acatgtgcag ctccctggct 3240

ctgccaacag gcctgacatc tctgatcctg ccaagcagcg atctggccgt gctgatctct 3300

agctccacca gccactttct gatgcggagc ccagtgctgc ctagctccag actgacttgt 3360

gcctctccac agctgcccag aatgtggacc tggtccagct ggctgaagtg gctgaaaaac 3420

tggaagcggt tcaagcttaa aaagaagtac atcctggcca tgacgctgac cctgaacggg 3480

catccaagaa ccgcctctat caccctgcct accaccagcc tgaagggctc tagaaccagc 3540

tgttgcccca gatctcaccc cagactgcct aggacaaagt ggcctctgca gtgcctgctg 3600

cacagcctgg gaacaactgg aacactcgtg tgggaaaggg gcgtgctgca gatgagaagt 3660

ctggaagctc actgccctct gcagaccttc cacctccacc actgtagaac tcagcccagc 3720

tcttgccctc ctgcatggat tctgtaccaa ggacagtgga gcggagccgt cgtgggactt 3780

agacctgtgg ctcaccatct gccaagacct attcagggcc tgcactgccc acaggctcca 3840

catcaaccag ctctgtcttg gggcaagtgg cccgtgggaa tgacaggacc tgccagatgt 3900

tgccggtggc ctagacggga actcaaaaag aatctgctgc tcccctgccg gtgcagacgc 3960

gagtatagag tgacaatgggg ccaagccttc ggcagcttta gcgccgactt tagcgctccc 4020

caggtgttga agaagaatcg gaagcagaac aagattatct gcaagggcca ccgcatcaca 4080

agcctgatcc tgagctttca gcgcgtgaaa cttaagaaga aagtcaacgc ctgctctccc 4140

gcctgcacct ctaaagcctg gactcccaga aagctcgtgg ggagagccgt cagaagaaag 4200

ggcatcatct gtatcaagaa tatgttcctg cagcggaagg gtttcctgaa cctgagccac 4260

gtggaaaagt tcgtgcctcc ttctcctcct ctggctctcg gccctagaat gcagctctgt 4320

acccagctgg cccggttctt ccctatcaca cctcctgtgt ggcacatcct gggacctcag 4380

aggcacaccc cttgtcaaaa gaaggtgccc tccgctagcc catggggcct tagaccatct 4440

ccttgcctga gctggtcctc cagaattgtg gcccctggac acgccctgag ctgtcggact 4500

agaagaagat ggtccagcaa g 4521

<210> 1104

<211> 29

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA amino acid sequence

<400> 1104

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

333

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg

20 25

<210> 1105

<211> 232

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Human Invariant chain (hINV) amino acid sequence

<400> 1105

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met

225 230

334

<210> 1106

<211> 11

<212> PROTEIN

<213> Artificial sequence

<220>

<223> HA tag amino acid sequence

<400> 1106

Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1 5 10

<210> 1107

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 1A poly-FSP

<400> 1107

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Thr Glu Ile

20 25 30

Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu

35 40 45

Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro

50 55 60

Gln Asp Ser Arg Gln Val Leu Ala Leu Ala Lys Lys Thr Leu Thr Asn

65 70 75 80

Ala Ser Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly

85 90 95

Met Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys

100 105 110

Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp His

115 120 125

Arg Ser Gly Leu Arg Ala Gln Met Pro Ser Ser Trp Ser Ser Ser Gly

130 135 140

Arg Thr Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr Leu Lys Leu

145 150 155 160

Ile Leu Val Met Leu Glu Ile Val Thr Glu Arg Ser Leu Leu Tyr Ser

165 170 175

Arg Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr

180 185 190

335

Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe Pro

195 200 205

Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys

210 215 220

Thr Ser His Ser Asn Pro Trp Ser Gln Ser Asn Gln Gln Leu Lys Trp

225 230 235 240

Thr Pro His Ile Leu Lys Ser Ala Ser Phe Asp Lys Lys Gly Phe Val

245 250 255

Ile Leu Ser Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile

260 265 270

Ala Ser Ser Pro Gly His Pro Leu Gly Pro Thr Leu Ala Ile Arg Phe

275 280 285

Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser

290 295 300

Gly Gln Tyr Phe Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn Asn

305 310 315 320

Met Gly Gly Asn Arg Ser Ile Cys Leu Thr Arg Lys Arg Lys Arg Glu

325 330 335

Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys

340 345 350

Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe

355 360 365

Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr

370 375 380

Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro His

385 390 395 400

Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser His His

405 410 415

Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly

420 425 430

Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Gln Arg Lys Val Val Lys

435 440 445

Asn Pro Ser Gln Pro Val Pro Leu Arg Asn Asp Gly Ile Thr Asn Gly

450 455 460

Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser Leu Lys Lys Ser Leu Glu

465 470 475 480

Lys Met Pro Leu Met Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys

336

485 490 495

Arg Met Trp Trp Lys Gly Lys Lys Arg Arg Lys Glu Ile Val Pro Met

500 505 510

Ser Leu Ala Cys Ser Ile Thr Val Ser Phe Val Gly Gln Ile Ser Thr

515 520 525

Leu Val Leu Asp Val Ser Ser Ile Ser His Trp Arg Leu Pro Cys Lys

530 535 540

Val Thr Glu Ser Ser Ser Ser Phe Phe Tyr Thr Ser Asp Arg Thr Pro

545 550 555 560

Leu Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu

565 570 575

Pro Gly Leu Thr His Pro Ala His Gln Pro Leu Gly Ser Met Ser Thr

580 585 590

Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp

595 600 605

Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys Leu Arg

610 615 620

Leu Arg Asn Thr Ser Pro Gly Glu Lys Asn Asp Leu Gln Leu Phe Val

625 630 635 640

Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn

645 650 655

Pro Cys Gly Asn Leu His Leu Lys Thr Thr Ser Leu Gln Arg Pro Pro

660 665 670

Arg Ser Ile Leu Ser Leu Asn Gly Ile Trp Glu Val Met Pro Arg Thr

675 680 685

Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr Ser Gln Asn His

690 695 700

Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu Asn Val Ser Lys

705 710 715 720

Lys Arg Leu Pro Lys Leu Met Lys Lys Lys Asp Ser Thr Ile Phe Ile

725 730 735

Asn Lys Val Arg Gln Asn Phe Lys Arg Asn Phe Lys Lys Lys Lys Pro

740 745 750

Leu Thr Phe Ser Pro Ser Arg Arg Met Thr Arg Arg Thr Met Thr Thr

755 760 765

Thr Lys Met Met Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg

770 775 780

337

Arg Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr

785 790 795 800

Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln

805 810 815

Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu

820 825 830

Ala Ile Trp Gly Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser

835 840 845

Gly Thr Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr Cys

850 855 860

Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala Pro

865 870 875 880

Val Gly Ala Gln Ala Pro Glu Val Thr Leu Arg Ala Ser Cys Ala

885 890 895

Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp Gly Ala Gln

900 905 910

Val Arg Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys Val Pro Val

915 920 925

Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala Ala Gly Thr

930 935 940

Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro Ala Ser Ser

945 950 955 960

Pro Thr Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg Arg Lys Arg

965 970 975

Ser Ser Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu Arg Met Met

980 985 990

Leu Pro Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro Gln Gly Arg

995 1000 1005

Ala Pro Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro Ser Leu

1010 1015 1020

Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu Ala

1025 1030 1035

Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro Ser Cys His

1040 1045 1050

Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln Ala Pro

1055 1060 1065

338

Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe Arg Asn

1070 1075 1080

Tyr Pro Arg Ala Asp Lys Lys Asn Val Arg Ser Thr Ala Phe Phe

1085 1090 1095

Leu Leu Asp Ile Ile Leu Gly Asp Cys Pro Arg Thr Met Ala Gly

1100 1105 1110

Pro Ser Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser Arg Lys

1115 1120 1125

Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro Ala

1130 1135 1140

Gly Trp Ala Arg Gln Ala Tyr Pro Arg Leu Lys Thr Phe Gly Val

1145 1150 1155

Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr Met

1160 1165 1170

Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser

1175 1180 1185

Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro Trp

1190 1195 1200

Arg Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro Thr

1205 1210 1215

Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg Met

1220 1225 1230

Pro Gly Ser Gln Ser Ile Met Ser Trp Met Pro Pro Leu Ala

1235 1240 1245

Pro Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys Pro

1250 1255 1260

Ala His Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr Pro

1265 1270 1275

Leu Ser Ala His Pro Val Pro Val Leu Ser Gly Cys His Leu Ala

1280 1285 1290

Val Arg Thr Ser Met Leu Thr Leu Leu Pro Met Glu Ala Lys Lys

1295 1300 1305

Ser Met Lys Arg Ser Asn Ala Lys Lys Met Glu Asp Met Ile Trp

1310 1315 1320

Glu Ser Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu

1325 1330 1335

339

His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Ile

1340 1345 1350

Val Phe Phe Leu Ala Pro Tyr Phe Pro Arg His Pro His Arg Ser

1355 1360 1365

Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro

1370 1375 1380

Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu

1385 1390 1395

Ala Thr Gly Leu His Leu His Arg Gln Ile Ser Leu Pro Leu Arg

1400 1405 1410

Gly Phe Leu Leu His Gln Pro Leu Pro Gly Gln His Leu Trp Leu

1415 1420 1425

Ser His Arg Leu Arg Leu Arg Leu Pro Arg Thr Gln Pro Ser Leu

1430 1435 1440

Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro Pro

1445 1450 1455

Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala Thr Leu Arg Cys

1460 1465 1470

Pro Ala Val Pro Ala Ala Ala Cys Pro Pro Ala Leu Arg Ser Met

1475 1480 1485

Pro Lys Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu Thr Pro

1490 1495 1500

Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro Gln

1505 1510 1515

Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln Ser

1520 1525 1530

Met Leu Val Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1108

<211> 1541

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 2A poly-FSP

<400> 1108

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Arg Lys Met

20 25 30

340

Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Gly Ser Pro Pro Leu

35 40 45

His Leu Cys Gln Pro Leu Arg Met Ser Arg Thr Pro Pro Arg Arg Val

50 55 60

Cys Ala Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Asn Pro

65 70 75 80

Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser

85 90 95

Gln Lys Asn His Leu Tyr Gly Gly Lys Lys Arg Ser Lys Phe Ser Arg

100 105 110

Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Val Leu

115 120 125

Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly Arg Cys Ser

130 135 140

Leu Met Glu Gln Ile Pro His Leu Lys Ser Arg Arg Lys Trp Gln Ile

145 150 155 160

Arg His Val Leu Lys Asn Leu Trp Leu Pro Met Arg Ser Leu Gly Glu

165 170 175

Pro Trp Arg Ala Gly Phe Gln Trp Gly Glu Ser Pro Trp Gln Val Met

180 185 190

Ala Ser Leu Thr Gly Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys

195 200 205

Ser Leu Pro Gln Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu

210 215 220

Leu Val Gln Ser Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile

225 230 235 240

Asn Ile His His Arg Lys Asn Pro Leu Leu Pro Met Arg Trp Trp Cys

245 250 255

Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys Val Ser Gly

260 265 270

Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly Ile Cys Trp

275 280 285

Lys Trp Pro Val Thr Val Ala Ala Ile Arg Met Cys Ala Leu Ser His

290 295 300

Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser Lys His Cys

305 310 315 320

341

Met Thr Ala Cys Thr Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu

325 330 335

Lys Gly Ala Ile Gln Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val

340 345 350

Val Asn Phe Glu Ala Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu

355 360 365

Lys Ile Thr Leu Phe Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn

370 375 380

Val Leu Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys

385 390 395 400

Arg Lys Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg

405 410 415

Leu Phe Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln

420 425 430

Asn Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile

435 440 445

Leu Thr Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln Val

450 455 460

Met Leu Cys Ala Ser Pro Gln Ile Met Gln Arg Thr Ser Ala Asp Arg

465 470 475 480

Phe Arg Met Lys Arg Arg Met Gln Asn Leu Asp Asn Leu Gly Val Pro

485 490 495

Trp Pro Leu Leu Pro Gly Ile Pro Ser Leu Asn Ala Gln Trp Met Phe

500 505 510

Leu Arg Ile Ala Ser Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn

515 520 525

Phe Glu Thr Ile Arg Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser

530 535 540

His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe

545 550 555 560

His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Leu Leu Leu Ala

565 570 575

His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys Val

580 585 590

Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln Pro

595 600 605

Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Gly His Arg Glu Gly Ser

342

610 615 620

Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Pro Tyr Pro

625 630 635 640

Ile Ser Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn

645 650 655

Ser Leu Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His

660 665 670

Ala Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser

675 680 685

Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys Lys

690 695 700

Phe Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr

705 710 715 720

Ala Asn Met Cys Trp Pro Lys Phe Phe Leu Thr Phe Ser Gln Gln Trp

725 730 735

Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys Ser

740 745 750

Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser Leu Arg Asp

755 760 765

Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg Val Leu Trp

770 775 780

Met Pro Thr Ser Thr Val Leu Gln Ala His Pro Gln Val Pro Ala Leu

785 790 795 800

Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala

805 810 815

Pro Ser Ala Gln Gln Ser Leu Thr Arg Glu Lys Ile Ile Asn Pro Thr

820 825 830

Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Ile Tyr Pro Pro

835 840 845

Ser Thr Cys Ser Leu Pro Cys Arg Gly Phe Leu Pro Ser Arg Phe Pro

850 855 860

Trp Gly Pro Pro Pro Leu Asn Ser Val Ile Val His Lys Ile Val Leu

865 870 875 880

Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu Arg Arg Ile Ser

885 890 895

Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu Lys Leu Leu Asn

900 905 910

343

Met Asp Leu Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu

915 920 925

Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val His Val

930 935 940

Leu Met Phe Gly Ala Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro

945 950 955 960

Gln Ala Gln Thr Pro His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser

965 970 975

Ser Ser Pro Arg Ala Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys

980 985 990

Trp Pro Arg Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Trp

995 1000 1005

Pro Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser

1010 1015 1020

Arg Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg Ser Ser Leu

1025 1030 1035

Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr Ser Ser

1040 1045 1050

Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg Arg Gln Ile Asn

1055 1060 1065

Arg Arg Lys Leu Gln Arg Lys Lys His Trp Asn Ser Ala Tyr Leu

1070 1075 1080

Gly Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr His Arg Trp Ile

1085 1090 1095

Val Thr Ser Met Cys Gln Ser Gln Arg Trp Leu Thr Ser Thr Thr

1100 1105 1110

Ser Arg Ser Ser Ala Leu Met Trp Thr Glu Leu Ser Leu Glu Pro

1115 1120 1125

Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Arg Thr Leu Glu

1130 1135 1140

Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln Cys Gln Glu Lys

1145 1150 1155

Lys Ser Arg Lys Leu Met Ser Ser Cys Thr His Pro Cys Thr Met

1160 1165 1170

Leu Leu Pro Gln Gly Cys His Pro Ala Trp Leu Ser Pro Ile Ser

1175 1180 1185

344

Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His Val Leu Ala Leu

1190 1195 1200

Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro Gln Met Gln Leu

1205 1210 1215

Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys Pro Gly Thr

1220 1225 1230

Ser Leu Gln Cys Tyr Leu Asn Arg Arg Arg Asn Ser Arg Lys Gln

1235 1240 1245

Ser Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp Lys His

1250 1255 1260

Lys Tyr Phe Leu Phe Gly Gln Lys Lys Gly His Met Ile Phe Ser

1265 1270 1275

Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Asn Ser Arg Lys

1280 1285 1290

Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr Gly Lys Gly

1295 1300 1305

Leu Thr His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn Lys Arg

1310 1315 1320

Asn Lys Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile Lys

1325 1330 1335

Arg Lys Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro Glu

1340 1345 1350

Ser Asn Leu Ile Leu Ile Gln His Gly Gly Ile Phe Thr Phe Arg

1355 1360 1365

Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu Ser

1370 1375 1380

Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr Ile

1385 1390 1395

Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe His Val Pro Val

1400 1405 1410

Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr Thr Glu Lys

1415 1420 1425

Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys Tyr

1430 1435 1440

Gln Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly

1445 1450 1455

345

Thr Pro Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg Ala Phe

1460 1465 1470

Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr Gly

1475 1480 1485

Cys Trp Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr Arg Arg

1490 1495 1500

Lys Lys Arg Glu Asp His Cys Gly Leu Arg His Pro Ser Trp Pro

1505 1510 1515

Trp Thr Arg Cys Leu Arg Met Arg Pro Pro Arg Ser Gly Tyr Pro

1520 1525 1530

Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540

<210> 1109

<211> 1546

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 3A poly-FSP

<400> 1109

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Gly Ala Ala

20 25 30

Ala Ala Ser Cys Gln Ser Pro Ser Pro Arg Glu Thr Thr Pro Ser

35 40 45

Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys

50 55 60

Thr Met Thr Ala Ala Gly Leu Thr Pro Met Ile Arg Lys Ile Gln Lys

65 70 75 80

Arg Arg Arg Thr Ala Asn Arg Cys Leu Arg Val Arg Cys Gly Gly Trp

85 90 95

Leu Cys Gly Trp Thr Pro Arg Gly Gln Asn Thr Arg Ser Thr Asn Leu

100 105 110

Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu

115 120 125

Ser Ser Leu Phe Phe Ser His Cys Leu Cys Arg Asn Ser Arg Arg Ala

130 135 140

Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Pro

145 150 155 160

346

Ile Pro Pro His Leu Arg His Pro Leu Leu Leu Met Asp Leu Asp Gly

165 170 175

Met Leu Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp

180 185 190

Leu Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln

195 200 205

Val Gln Thr Gly Gly Arg Leu Thr Pro Pro Pro Pro Leu Ile Leu Trp Pro

210 215 220

Gln Ala Ala Pro Val Asp Pro Pro Gly Ser Val Trp Ile Leu Pro Cys

225 230 235 240

Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys Arg Leu Asp Phe His

245 250 255

Pro Arg Ser Ser Ser Gly Val Ser Val Ile Thr Met Lys Leu Trp Glu

260 265 270

Lys His Phe Lys Gly Leu Asn Trp Asn Leu Val Val Trp Ile Leu Asn

275 280 285

Leu Lys Met Met Asn Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala Arg

290 295 300

Lys Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro Ser Glu Lys Lys

305 310 315 320

Arg Lys Leu Pro Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser

325 330 335

Arg His Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe

340 345 350

Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln

355 360 365

Phe Leu Ile His Leu Arg Lys Val Asp Thr His His Leu Gln Val Val

370 375 380

Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg

385 390 395 400

Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala

405 410 415

Ala Gln Ala Pro Pro Lys Ser His Phe Pro Pro His Pro Ser Gly Pro

420 425 430

Ser Arg Thr Ser Ser Leu Gln Leu Arg Ser Ile Pro His Val Pro Lys

435 440 445

347

Ala Thr Ala Ala His Ser Val Thr Val Lys Leu Gln Lys Gly Lys Asn

450 455 460

Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln Gly His Leu

465 470 475 480

Leu Met Ile Leu Leu His Asn Thr Tyr Met Glu Met His Tyr Gly His

485 490 495

Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu Leu Cys Arg Gln Met

500 505 510

Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys

515 520 525

Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly

530 535 540

Val Pro Gln Thr Ala Ala Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr

545 550 555 560

Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met Ser Cys Ser

565 570 575

Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu

580 585 590

Leu His Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala

595 600 605

Cys Arg Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala

610 615 620

Thr Trp Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser Arg

625 630 635 640

Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Ser Arg Arg Asn Arg

645 650 655

Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Val

660 665 670

Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser Pro Gly Pro Thr

675 680 685

Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala Ile Gly Lys Leu Ala

690 695 700

Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala Arg Glu Ala

705 710 715 720

Gly Arg Phe Ala Val Leu Gly Thr Met Val Met Lys His Leu Asn Arg

725 730 735

Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr Met Met Met

348

740 745 750

Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser

755 760 765

Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro

770 775 780

Cys Leu Leu Leu Leu His His Arg His His Leu Ala Phe Leu Glu Met

785 790 795 800

Ala Cys Ser Ser Gln His Thr His Ser Ile Lys Ile Trp Ile Ser Phe

805 810 815

Ser His Glu Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met

820 825 830

Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His

835 840 845

His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp

850 855 860

Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp

865 870 875 880

Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly Lys Arg Cys Asn

885 890 895

Pro Cys Cys Arg Leu Thr Trp Met Ser Met Leu Gln Lys Gln Cys Cys

900 905 910

Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg

915 920 925

Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly Phe Leu

930 935 940

Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys Leu Leu Met

945 950 955 960

Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Arg Glu Trp Glu Lys

965 970 975

Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu

980 985 990

Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val Leu Ala Gly Asp Lys

995 1000 1005

Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln Ser Glu Thr Lys

1010 1015 1020

Ala Val Ser Leu Gln Leu Pro Leu Gly Arg Gly Pro Val Tyr Tyr

1025 1030 1035

349

Val Cys Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Phe Leu

1040 1045 1050

Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys Thr Leu Gly Arg

1055 1060 1065

Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys Leu Ile Pro

1070 1075 1080

Val Pro Ile Asp Leu Pro Arg Met Leu Pro Ile Ser Ser Pro Leu

1085 1090 1095

His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr Phe Pro Ala Thr

1100 1105 1110

Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp Gly Trp Arg Thr

1115 1120 1125

Phe Arg Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro

1130 1135 1140

Ala Asn Gln Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu

1145 1150 1155

Arg Thr Glu Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser

1160 1165 1170

Phe Leu Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln Met Thr

1175 1180 1185

Asn Val Gly Cys Ala Phe Ser Gly Arg Ile Leu His Gln Pro Glu

1190 1195 1200

Met Ala Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr Asn

1205 1210 1215

Ser Trp Trp Thr Cys Thr Asn Leu Ser Val Pro Met Met Leu Thr

1220 1225 1230

Ile Leu Ile Trp Lys Arg Val Phe Ile Leu Leu Leu Ser Asp Lys

1235 1240 1245

Lys Leu Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn

1250 1255 1260

Val Ser Arg Ile Ser His Gln Met Lys Cys Leu Thr Ala Val Asp

1265 1270 1275

Cys Leu Gln Ala Phe Trp Asp Met Asn Met Glu His Arg Leu Thr

1280 1285 1290

Met Asp Ser Ala Leu Ser Leu Thr Lys Arg Glu Gly Pro Glu Thr

1295 1300 1305

350

Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu Thr Met Ser Leu Ser

1310 1315 1320

Asn Ile Leu Met Lys Phe Arg Leu Arg Leu Arg Ile Pro Ser Leu

1325 1330 1335

Arg Lys Tyr Arg Gln His Leu Lys Lys Thr Ser Ala Lys Gly Glu

1340 1345 1350

Lys Leu Lys His Cys Phe Lys Ala Trp Gly Arg Gly Pro Pro Leu

1355 1360 1365

His Thr Arg Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys

1370 1375 1380

Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu Arg Arg Asn Leu

1385 1390 1395

Gln Arg Asn Leu Lys Val Leu Trp Leu Lys Lys Ser Thr Lys Ser

1400 1405 1410

Arg Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Phe Glu Lys Lys

1415 1420 1425

Arg Pro Glu Trp Leu Ser Ser Arg Lys Lys Gly His Thr Lys Asn

1430 1435 1440

Arg Arg Lys Ala Lys Arg Lys Pro Gln Ile Val His Lys Lys Arg

1445 1450 1455

Gly Leu Phe Leu Lys Lys Lys Tyr Leu Val Gln Gly Ala Lys Lys

1460 1465 1470

Ile Thr Phe Leu Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro

1475 1480 1485

Thr Pro Arg Leu Ser Asp Arg Val Phe Leu Val Lys Ile Thr Thr

1490 1495 1500

Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg Gly

1505 1510 1515

Gly Thr Thr Ile Gly Glu Val Thr Ser Leu Cys Phe Phe Tyr Leu

1520 1525 1530

Leu Tyr Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1110

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 4A poly-FSP

351

<400> 1110

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Ile Gln Lys

20 25 30

Lys Lys Gln Lys Ser Met Ala Lys Lys Ser Gln Gln Pro Leu Val Leu

35 40 45

Lys Lys Asn Arg Lys Gln Glu Trp Gly Gly Gln Ala His Arg Gln Arg

50 55 60

Asn Asn Ala Lys Lys Met Pro Phe Gln Glu Glu Lys Lys Asn Gly Arg

65 70 75 80

Ile Ser Ser Ser Leu Ile Leu Ser Phe Gln Arg Val Lys Asp Ser Lys

85 90 95

Lys Met Thr Gln Lys Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu

100 105 110

Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu Ile Ser Ser

115 120 125

Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro Ser Ser Arg

130 135 140

Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp Thr Trp Ser Ser

145 150 155 160

Trp Leu Lys His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser

165 170 175

Leu Lys Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu

180 185 190

Pro Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr

195 200 205

Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg Ser Leu

210 215 220

Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His His Cys Arg Thr

225 230 235 240

Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp

245 250 255

Ser Gly Ala Val Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg

260 265 270

Pro Ile Gln Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu

275 280 285

352

Ser Trp Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys

290 295 300

Arg Trp Pro Arg Arg Ser Gln Lys Ser Gln His His Cys Ala Leu Arg

305 310 315 320

Lys Ile Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro

325 330 335

Gln Ala Gln Glu Lys Ala Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser

340 345 350

Arg Ile Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg

355 360 365

Trp Ser Ser Lys Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys

370 375 380

Ser Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val

385 390 395 400

Gln Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly

405 410 415

Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu

420 425 430

Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro

435 440 445

Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val Thr Val Met

450 455 460

Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro

465 470 475 480

Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp

485 490 495

Arg Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Cys Gln Lys Lys Val

500 505 510

Pro Ser Ala Ser Pro Trp Gly Leu Val Pro Ser Pro Pro Leu Ala

515 520 525

Leu Gly Pro Arg Met Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro

530 535 540

Ile Thr Pro Pro Val Trp His Ile Leu Gly Pro Gln Arg His Thr Pro

545 550 555 560

Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe Phe Arg Gly Ser

565 570 575

353

Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys Asn Glu Gly Asn Asn

580 585 590

His Trp Pro Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr

595 600 605

Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys

610 615 620

His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln

625 630 635 640

Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu

645 650 655

Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys

660 665 670

Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln

675 680 685

His Val Trp Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly

690 695 700

Phe Cys His Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser

705 710 715 720

Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser

725 730 735

Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly

740 745 750

Val Trp Ala Arg Leu Leu Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile

755 760 765

Ser Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr

770 775 780

Asp Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly Val

785 790 795 800

Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg Cys

805 810 815

Arg Cys Arg Arg Glu Tyr Arg Val Thr Met Asn Ile Leu Asn Ser Leu

820 825 830

Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu Thr Ala Lys Lys Ser

835 840 845

Pro Thr Ser Phe Cys Cys Asn Leu Asp Leu Gln His Gln Arg Arg Arg

850 855 860

Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro Lys Ala

354

865 870 875 880

Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn Leu Leu

885 890 895

Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys Asp Glu

900 905 910

Glu Asp His Gln Lys Arg His His His His Asn Gln Lys Lys Met Ser

915 920 925

Val Pro Leu Phe Phe His Gly Asp Leu Cys Arg Glu Ser Thr Leu Gly

930 935 940

Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Gln Arg Pro Pro Leu Gln

945 950 955 960

Pro Val Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu Lys Met Glu

965 970 975

Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu Cys Thr Ser

980 985 990

Arg Ile Leu Asn Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met

995 1000 1005

Lys Leu Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys

1010 1015 1020

Leu Glu Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser

1025 1030 1035

Tyr Lys Ile Thr His Pro Phe Ala Ser Gln Pro Leu Gly Gln Glu

1040 1045 1050

Met Pro Asn Asn Thr Gln Ile Arg Leu Leu Lys Pro Asp Lys Lys

1055 1060 1065

Lys Lys Ser Thr Glu Lys Thr Lys Gly Gln Ala Phe Gly Ser Phe

1070 1075 1080

Ser Ala Asp Phe Ser Ala Pro Gln Gly Gln Ala Phe Gly Ser Leu

1085 1090 1095

Cys Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr Arg

1100 1105 1110

Leu Arg Ser Leu Asn His Leu Leu Ile Trp Leu Lys Asn Trp Lys

1115 1120 1125

Arg Phe Lys Leu Lys Lys Lys Tyr Gln Ala Pro Leu Arg Leu Trp

1130 1135 1140

Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser Ile Ile Cys

1145 1150 1155

355

Ile Lys Asn Met Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu Ser

1160 1165 1170

His Val Glu Lys Phe Glu Leu Lys Lys Asn Leu Leu Leu Pro Val

1175 1180 1185

Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Met Leu Asn Gln Lys

1190 1195 1200

Cys Leu Ala Pro Phe Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro

1205 1210 1215

Arg Lys Leu Val Gly Arg Ala Val Arg Arg Lys Gly Ile Leu Ala

1220 1225 1230

Met Thr Leu Thr Leu Asn Gly Ile Glu Ala Leu Thr Ala Leu Phe

1235 1240 1245

Thr Cys Arg Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met

1250 1255 1260

Ser Ile Leu Glu Ala Ile Ile Lys Lys Met Lys Lys Arg Ile Met

1265 1270 1275

Val His Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met Leu

1280 1285 1290

Cys Leu Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser Asn

1295 1300 1305

Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys Gln

1310 1315 1320

Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro Asn

1325 1330 1335

Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp His

1340 1345 1350

Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser Arg

1355 1360 1365

Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met Ala

1370 1375 1380

Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp Cys

1385 1390 1395

Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Ser Pro Leu

1400 1405 1410

Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu Trp

1415 1420 1425

356

Glu Ile Pro Ser Val Thr Leu Leu Ala Asn Leu His Leu Cys Tyr

1430 1435 1440

Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg Gln Met Glu Ser

1445 1450 1455

Leu Gly Met Lys Leu Gln Leu Lys Lys Lys Val Asn Ala Cys Ser

1460 1465 1470

Thr Tyr Thr Pro His Ile Cys Arg His Thr Cys Arg Val Asn Ala

1475 1480 1485

Val Glu Glu Leu His Ser Arg Arg Met Ser Val Arg Ile Val Lys

1490 1495 1500

Asn Trp Val Ser Cys Cys Tyr Val Arg Leu Ser Ala Val Gly Leu

1505 1510 1515

Ser Thr Trp Ser Ala Leu Asp Asn Lys Ile Ile Cys Lys Gly His

1520 1525 1530

Arg Ile Thr Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1111

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 1B poly-FSP

<400> 1111

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Thr Glu Ile

20 25 30

Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu

35 40 45

Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro

50 55 60

Gln Asp Ser Arg Gln Val Leu Ala Met Pro Ser Ser Trp Ser Ser Ser

65 70 75 80

Gly Arg Thr Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro

85 90 95

Leu Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys

100 105 110

Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met Met Lys

115 120 125

357

Lys Lys Asp Ser Thr Ile Phe Ile Val Pro Met Ser Leu Ala Cys Ser

130 135 140

Ile Thr Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val

145 150 155 160

Ser Ser Ile Ser His Trp Asn Lys Val Arg Gln Asn Phe Lys Arg Asn

165 170 175

Ala Asp Lys Lys Asn Val Arg Ser Gly Glu Lys Asn Asp Leu Gln Leu

180 185 190

Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu

195 200 205

Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr Thr Ser Leu Gly Asp

210 215 220

Cys Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro Ala Ser

225 230 235 240

Gly Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg

245 250 255

Ala Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Phe Asp Lys Lys Gly

260 265 270

Phe Val Ile Leu Ser Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys

275 280 285

Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala

290 295 300

Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro

305 310 315 320

Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg

325 330 335

Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu

340 345 350

Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro

355 360 365

Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro

370 375 380

Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu

385 390 395 400

Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro Ser Cys His

405 410 415

358

Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln Ala Pro Thr

420 425 430

Ala Phe Phe Leu Leu Asp Ile Ile Leu Arg Leu Pro Cys Lys Val Thr

435 440 445

Glu Ser Ser Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr Asn Ala

450 455 460

Lys Lys Met Glu Asp Met Ile Trp Glu Ser Thr Pro Leu Ser Ile Ser

465 470 475 480

Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu Thr

485 490 495

His Pro Ala His Gln Pro Leu Gly Ser Met Thr Leu Ala Ile Arg Phe

500 505 510

Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser

515 520 525

Gly Gln Tyr Phe Lys Gly Lys Lys Arg Arg Lys Glu Ile Phe Lys Lys

530 535 540

Lys Lys Pro Leu Thr Phe Ser Pro Ser Leu Ala Lys Lys Thr Leu Thr

545 550 555 560

Asn Ser Ser Phe Phe Tyr Thr Ser Asp Arg Glu Ala Lys Lys Ser Met

565 570 575

Lys Arg Ser Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe

580 585 590

Arg Asn Tyr Pro Arg Ala Ser Ser Pro Gly His Pro Leu Gly Pro Asn

595 600 605

Gly Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys Ile Ser Val

610 615 620

Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile Thr Glu Cys

625 630 635 640

Val Arg Thr Ala Leu Asn Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile

645 650 655

Gly Ser Ile Ala Leu Arg Ser Met Pro Lys Ser Arg Asn Gln Val Leu

660 665 670

Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro

675 680 685

Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln

690 695 700

359

Ile Pro Ser Gln Ser Met Leu Val Ala Ser Arg Ala Gly Gln Phe Pro

705 710 715 720

Val Trp Arg Arg Thr Ser Arg Gly Met Ser Leu Gln Trp Lys Ser Gly

725 730 735

Asn Ala Cys Thr Pro Leu Arg Cys Pro Ser Arg Ser Pro Ala Thr Phe

740 745 750

Leu Thr Ala Leu Asn Gly Trp His Arg Ser Gly Leu Arg Ala Gln Arg

755 760 765

Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu

770 775 780

Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg

785 790 795 800

Ser Pro Trp Ile Phe Val Ser Lys Lys Arg Leu Pro Lys Leu Thr Lys

805 810 815

Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile

820 825 830

Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro His Arg Arg

835 840 845

Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser His His Pro Ser

850 855 860

Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg

865 870 875 880

Arg Glu Lys Lys Gly Cys Arg Glu Arg Ser Leu Leu Tyr Ser Arg Met

885 890 895

Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr Phe Val

900 905 910

Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe Pro Val Gly

915 920 925

Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys Thr Ser

930 935 940

His Ser Asn Pro Trp Ser Gln Ser Arg His Pro His Arg Ser Pro Pro

945 950 955 960

Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro Arg Ala Ser

965 970 975

Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala Thr Gly Leu

980 985 990

His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe Leu Leu His

360

995 1000 1005

Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser His Arg Leu Arg

1010 1015 1020

Leu Arg Leu Pro Arg Thr Gln Arg Pro Pro Arg Ser Ile Leu Ser

1025 1030 1035

Leu Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu His

1040 1045 1050

Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Tyr Pro

1055 1060 1065

Arg Leu Lys Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys Leu

1070 1075 1080

Ala Gly Ser Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg Gln

1085 1090 1095

Glu Cys Gly Arg Arg Ala Ser Val Pro Cys Arg Pro Met Ile Gly

1100 1105 1110

Ser Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser Ala

1115 1120 1125

Met Gly Val Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser Pro

1130 1135 1140

Pro Ala Thr Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met Met

1145 1150 1155

Ser Trp Met Pro Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys Ser

1160 1165 1170

Pro Ala Pro Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro Thr

1175 1180 1185

Ala Val Pro Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro Val

1190 1195 1200

Leu Ser Gly Cys His Leu Ala Val Arg Thr Ser Met Leu Thr Leu

1205 1210 1215

Leu Pro Met Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr Leu

1220 1225 1230

Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Ser Thr Arg

1235 1240 1245

Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp

1250 1255 1260

Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys Leu

1265 1270 1275

361

Arg Leu Arg Asn Thr Ser Pro Ile Val Phe Phe Leu Ala Pro Tyr

1280 1285 1290

Phe Pro Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met

1295 1300 1305

Met Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg

1310 1315 1320

Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys

1325 1330 1335

Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln

1340 1345 1350

Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser

1355 1360 1365

Leu Ala Ile Trp Gly Asn Gln Gln Leu Lys Trp Thr Pro His Ile

1370 1375 1380

Leu Lys Ser Ala Ser Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr

1385 1390 1395

Cys Arg Met Trp Trp Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg

1400 1405 1410

Asn Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly Thr

1415 1420 1425

Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr Cys Pro

1430 1435 1440

Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala Pro

1445 1450 1455

Val Gly Ala Gln Ala Pro Pro Glu Val Thr Leu Arg Ala Ser Cys

1460 1465 1470

Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp Gly

1475 1480 1485

Ala Gln Val Arg Gln Pro Ser Leu Pro Gly Pro Cys Ala Ser Leu

1490 1495 1500

Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro Ser Gln Val

1505 1510 1515

Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro Ala Ala Ala

1520 1525 1530

Cys Pro Pro Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

362

<210> 1112

<211> 1541

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 2B poly-FSP

<400> 1112

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Arg Lys Met

20 25 30

Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Met Ser Arg Thr Pro

35 40 45

Pro Arg Arg Val Cys Asn Pro Arg Arg Lys Thr Trp Lys Met Arg Lys

50 55 60

Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn His Leu Tyr Asn Ser Arg

65 70 75 80

Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr Gly His Arg

85 90 95

Glu Gly Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro

100 105 110

Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe Tyr Ile Ser Ser Thr

115 120 125

Val Cys Phe Leu Ser Lys His Cys Met Thr Ala Cys Thr Gln Lys Lys

130 135 140

Gly His Met Ile Phe Ser Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser

145 150 155 160

Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Glu Leu Ser Leu Glu Pro

165 170 175

Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Gly Gly Lys Lys Arg

180 185 190

Ser Lys Phe Pro Gly Thr Ser Leu Gln Cys Tyr Leu Asn Arg Cys Ser

195 200 205

Leu Met Glu Gln Ile Pro His Leu Lys Ser Ala Ser Pro Gln Ile Met

210 215 220

Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn

225 230 235 240

Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly Pro Leu Glu

245 250 255

363

Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys Ile Thr Met Val

260 265 270

Leu Thr Tyr Pro Asn Cys Val Val His Val Leu Met Phe Trp Leu Pro

275 280 285

Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln Trp Gly Glu

290 295 300

Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Pro Tyr Pro Ile Ser

305 310 315 320

Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu

325 330 335

Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His Ala Ile

340 345 350

Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser Ser Lys

355 360 365

Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys Lys Phe Arg

370 375 380

Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala Arg

385 390 395 400

Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser Ala Ala

405 410 415

Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Ser Arg Ser His Leu

420 425 430

Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Leu Ala Asn Gly Leu

435 440 445

Trp Phe Asn Leu Ile Phe Val Leu Phe Lys Ala Asp Gln Ser Glu Ser

450 455 460

Ser Leu Ser Ser Gly Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu

465 470 475 480

Ile Asn Ile His His Arg Lys Asn Pro Leu Leu Pro Met Arg Ile Pro

485 490 495

Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Leu Leu Leu Ala His

500 505 510

Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys Val Cys

515 520 525

Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln Pro Leu

530 535 540

364

Asp Leu Lys Ile Ser Lys Thr Phe Met Leu Gln Ala His Pro Gln Val

545 550 555 560

Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu

565 570 575

Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu Thr Ala Ser Pro Gln Ile

580 585 590

Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Ile Tyr Pro Pro

595 600 605

Ser Thr Cys Ser Leu Pro Cys Arg Gly Arg Thr Leu Glu Ser Ala Gly

610 615 620

Leu Phe Pro Trp Leu His Thr Gln Cys Thr His Pro Cys Thr Met Leu

625 630 635 640

Leu Pro Gly Gly Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys

645 650 655

Lys Met Cys Lys Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys

660 665 670

Pro Trp Lys Gly Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Gln

675 680 685

Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys

690 695 700

Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser Leu Arg

705 710 715 720

Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg Val Leu

725 730 735

Trp Met Pro Thr Ser Thr Val Arg Arg Arg Asn Ser Arg Lys Gln Ser

740 745 750

Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp Lys His Lys Tyr

755 760 765

Phe Leu Phe Gly Arg Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser

770 775 780

His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe

785 790 795 800

His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Phe Leu Pro Ser

805 810 815

Arg Phe Pro Trp Gly Pro Pro Pro Leu Cys His Pro Ala Trp Leu Ser

820 825 830

365

Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His Val Leu

835 840 845

Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro Gln Met Gln

850 855 860

Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys Arg Lys Arg

865 870 875 880

Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln Glu Lys Gly

885 890 895

Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala Cys Arg Glu

900 905 910

Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe Glu Ala His

915 920 925

Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg Ala Ser Leu

930 935 940

Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg Asn Gln Val

945 950 955 960

Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly Tyr Gln Asn

965 970 975

Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe Ser Ser Phe

980 985 990

Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr Arg Arg Lys Lys Arg Glu

995 1000 1005

Asp His Cys Gly Leu Leu Ile Leu Ile Gln His Gly Gly Ile Phe

1010 1015 1020

Thr Phe Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys Lys

1025 1030 1035

Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu Tyr

1040 1045 1050

Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe His

1055 1060 1065

Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr

1070 1075 1080

Thr Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln Gln

1085 1090 1095

Gln Cys Tyr Gln Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser

1100 1105 1110

Cys Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val

366

1115 1120 1125

Tyr Ala Asn Met Cys Trp Ala Phe Trp Lys Pro Pro Ser Gln Gly

1130 1135 1140

Ile Ser Ser Cys Leu Met Thr Gly Cys Trp Gly Ser Pro Pro Leu

1145 1150 1155

His Leu Cys Gln Pro Leu Arg Arg Tyr Lys Lys Arg Gly Val Ile

1160 1165 1170

Ala Trp Arg Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn

1175 1180 1185

Leu Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln Val

1190 1195 1200

Met Leu Cys Gly Lys Gly Leu Thr His Pro Val Pro Leu Lys Arg

1205 1210 1215

Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val Asn Gln

1220 1225 1230

Lys Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu Ile

1235 1240 1245

Val Leu Asn Ser Pro Glu Ser Asn Pro Lys Phe Phe Leu Thr Phe

1250 1255 1260

Ser Gln Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr Gly Ala

1265 1270 1275

Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln Thr

1280 1285 1290

Pro His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro

1295 1300 1305

Arg Ala Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro

1310 1315 1320

Arg Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Trp Pro

1325 1330 1335

Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser Arg

1340 1345 1350

Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg Ser Ser Leu Trp

1355 1360 1365

Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr Ser Ser Gly

1370 1375 1380

Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr Pro

1385 1390 1395

367

Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg Asn Ser Val Ile

1400 1405 1410

Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr

1415 1420 1425

Asn Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile Glu

1430 1435 1440

Phe Ala Val Leu Lys Leu Leu Asn Met Asp Leu His Trp Asn Ser

1445 1450 1455

Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr His

1460 1465 1470

Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp Leu Thr

1475 1480 1485

Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr Arg Gln Ile

1490 1495 1500

Asn Arg Arg Lys Leu Gln Arg Lys Lys Arg His Pro Ser Trp Pro

1505 1510 1515

Trp Thr Arg Cys Leu Arg Met Arg Pro Pro Arg Ser Gly Tyr Pro

1520 1525 1530

Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540

<210> 1113

<211> 1546

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 3B poly-FSP

<400> 1113

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Gly Ala Ala

20 25 30

Ala Ala Ser Cys Gln Ser Pro Ser Pro Arg Glu Thr Thr Pro Ser

35 40 45

Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys

50 55 60

Thr Met Thr Ala Ala Gly Leu Thr Pro Lys Asn Lys Lys Arg Arg Lys

65 70 75 80

Leu Arg Pro Lys Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala

368

85 90 95

Asn Arg Arg Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu

100 105 110

Arg Arg Lys Tyr Leu Arg Val Arg Gly Pro Pro Leu His Thr Arg Asn

115 120 125

Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe

130 135 140

Pro Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys Ser Ile

145 150 155 160

Pro His Val Pro Lys Ala Thr Ala Ala His Ser Val Thr Val Lys Leu

165 170 175

Gln Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His Thr

180 185 190

Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg Leu Gln Ser Ser Pro

195 200 205

Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn Asn Lys Lys Met

210 215 220

Ala Met Thr Val Ile Ala Gly Ala His His Cys Gln Tyr Lys Leu Thr

225 230 235 240

Tyr Thr Lys Val Trp Lys Met Asn Trp Lys Lys Met Met Ile Gly Ser

245 250 255

Gln His Leu Leu Phe Ser Pro Leu Asp Ser Ser Pro Leu Gln Leu Leu

260 265 270

Leu His Pro His Gly Lys Arg Cys Asn Pro Cys Cys Arg Leu Thr Trp

275 280 285

Met Ser Gln Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu

290 295 300

Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu Ser Ala Phe Ser Gly Arg

305 310 315 320

Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met Pro Lys Phe

325 330 335

Leu Asn Ser Thr Asn Ser Trp Trp Thr Leu Thr Thr Leu Ser Phe Val

340 345 350

Leu Ile Glu Glu Ala Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro

355 360 365

Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu

370 375 380

369

Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu

385 390 395 400

His Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys

405 410 415

Arg Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr

420 425 430

Trp Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn Val Ser Ser Val

435 440 445

Leu Lys Pro Phe Leu Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys

450 455 460

Thr Leu Gly Arg Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys

465 470 475 480

Leu Ile Pro Val Pro Ile Ser Glu Lys Lys Arg Lys Leu Pro Asn Ile

485 490 495

Glu Ile Thr Pro Gln Arg Ser Lys Ala Met Leu Gln Lys Gln Cys Cys

500 505 510

Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg

515 520 525

Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly Phe Leu

530 535 540

Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys Leu Leu Met

545 550 555 560

Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Ser Arg Lys Lys Gly

565 570 575

His Thr Lys Asn Arg Arg Lys Ala Lys Arg Lys Pro Gln Ile Tyr Leu

580 585 590

Phe Phe Leu Thr Val Cys Gly Phe His Leu Lys Lys Thr Ser Ala Lys

595 600 605

Trp Leu Lys Lys Ser Thr Lys Ser Arg Ser Asn Gly Met Glu Ala Cys

610 615 620

Gly Thr His Pro Thr Pro Arg Leu Ser Cys Leu Arg Val Arg Cys Gly

625 630 635 640

Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Arg Lys Ser Gly Val Met

645 650 655

Lys Cys Trp Asp Leu Cys Pro Val His Lys Lys Arg Gly Leu Phe Arg

660 665 670

370

Leu Asp Phe His Pro Arg Ser Ser Ser Gly Lys Gly Lys Asn Ser Pro

675 680 685

Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln Gly His Leu Leu Met

690 695 700

Ile Leu Leu His Asn Asp Arg Val Phe Leu Val Lys Ile Gly Glu Lys

705 710 715 720

Leu Lys His Cys Phe Lys Ala Trp Gly Ser Arg Arg Asn Arg Arg Tyr

725 730 735

Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Phe Leu Ile

740 745 750

His Leu Arg Lys Val Asp Thr His Leu Gln Val Val Thr Lys Thr

755 760 765

Val Thr Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys

770 775 780

Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln Ala

785 790 795 800

Pro Pro Lys Ser His Phe Pro Pro His Pro Ser Gly Pro Ser Arg Thr

805 810 815

Ser Ser Leu Gln Leu Arg Ser Leu Phe Phe Ser His Cys Leu Cys Ser

820 825 830

Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys Leu Leu Lys Lys Ser

835 840 845

Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser Arg Ile Ser Thr Pro

850 855 860

Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro Val Ser Val Ile Thr

865 870 875 880

Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn Leu Val

885 890 895

Val Trp Ile Leu Asn Leu Lys Met Met Thr Tyr Met Glu Met His Tyr

900 905 910

Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu Leu Cys Arg

915 920 925

Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His Pro Gln Gly

930 935 940

Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser Val Leu Leu

945 950 955 960

371

Leu Gly Val Pro Gln Thr Ala Arg Gly Pro Arg Arg Lys Val Leu Met

965 970 975

Val Leu Trp Leu Pro Ala Asn Gln Ser Leu Gln Lys Ser Gln Val Phe

980 985 990

Gln Trp Val Leu Arg Thr Glu Val Ala Pro Arg Cys Pro Arg Ser Ala

995 1000 1005

Val Asp Ile Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys

1010 1015 1020

Ala Ser Val Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser Ala

1025 1030 1035

Pro Cys Gly Ala Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val

1040 1045 1050

Leu Gly Thr Met Val Met Lys Leu Cys Phe Phe Tyr Leu Leu Tyr

1055 1060 1065

Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln His Leu Asn

1070 1075 1080

Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr Met

1085 1090 1095

Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile Pro

1100 1105 1110

Leu Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His

1115 1120 1125

Pro Pro Pro Pro Cys Leu Leu Leu Leu His His Arg His His Leu

1130 1135 1140

Ala Phe Leu Glu Met Ala Cys Ser Ser Gln His Thr His Ser Ile

1145 1150 1155

Lys Ile Trp Ile Ser Phe Ser His Glu Val Asp Pro Pro Gly Ser

1160 1165 1170

Val Trp Ile Leu Pro Cys Asp Leu Pro Arg Met Leu Pro Ile Ser

1175 1180 1185

Ser Pro Leu His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr Phe

1190 1195 1200

Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp Gly

1205 1210 1215

Trp Arg Thr Phe Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser

1220 1225 1230

Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met

372

1235 1240 1245

Arg Leu Tyr Gln Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu Thr

1250 1255 1260

Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg

1265 1270 1275

Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Cys Thr Asn Leu Ser

1280 1285 1290

Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val Phe Ile

1295 1300 1305

Leu Leu Leu Ser Asp Lys Lys Gly Ala Lys Lys Ile Thr Phe Leu

1310 1315 1320

Pro Ile Pro Pro His Leu Arg His Pro Leu Leu Leu Phe Glu Lys

1325 1330 1335

Lys Arg Pro Glu Trp Leu Ser Met Asp Leu Asp Gly Met Leu Thr

1340 1345 1350

Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu Gly

1355 1360 1365

Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln Val

1370 1375 1380

Gln Thr Gly Gly Arg Leu Arg Arg Asn Leu Gln Arg Asn Leu Lys

1385 1390 1395

Val Leu Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser

1400 1405 1410

Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Arg Glu Trp

1415 1420 1425

Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu

1430 1435 1440

Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val Leu

1445 1450 1455

Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln

1460 1465 1470

Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg Asn

1475 1480 1485

Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu Thr Lys

1490 1495 1500

Arg Glu Gly Pro Glu Thr Leu Lys Lys Lys Tyr Leu Val Gln His

1505 1510 1515

373

Gln Met Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe Trp

1520 1525 1530

Asp Met Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1114

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA- extended polypeptide 4B poly-FSP

<400> 1114

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Ile Gln Lys

20 25 30

Lys Lys Gln Lys Ser Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys

35 40 45

Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn

50 55 60

Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn

65 70 75 80

Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys Asp Glu Glu

85 90 95

Asp His Gln Lys Arg His His His His Asn Gln Lys Lys Met Ser Val

100 105 110

Met Ala Lys Lys Ser Gln Gln Pro Leu Gly Gln Ala Phe Gly Ser Leu

115 120 125

Cys Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr Arg Leu

130 135 140

Arg Ser Leu Asn His Leu Leu Ile Asn Glu Gly Asn Asn His Trp Pro

145 150 155 160

Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg

165 170 175

Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys His Trp Gln

180 185 190

Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr

195 200 205

Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro

374

210 215 220

Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser

225 230 235 240

Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp

245 250 255

Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly Phe Cys His

260 265 270

Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr

275 280 285

Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr

290 295 300

Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala

305 310 315 320

Arg Leu Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly

325 330 335

Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg

340 345 350

Asn Ile Leu Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln

355 360 365

Glu Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr

370 375 380

Asp Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys

385 390 395 400

Ser Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln Ser Pro Leu Trp

405 410 415

Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu Trp Glu Ile

420 425 430

Pro Ser Val Thr Leu Leu Ala Cys Arg Val Asn Ala Val Glu Glu Leu

435 440 445

His Ser Arg Arg Met Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys

450 455 460

Cys Tyr Val Arg Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu

465 470 475 480

Asp Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Ser Gln Lys Ser

485 490 495

Gln His His Cys Ala Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser

500 505 510

375

Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys Ala Asn Ala Lys

515 520 525

Lys Met Pro Phe Gln Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln

530 535 540

Met Leu Cys Leu Pro Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg

545 550 555 560

Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu

565 570 575

Ala Pro Leu Phe Phe His Gly Asp Leu Cys Glu Glu Lys Lys Asn Gly

580 585 590

Arg Ile Ser Ser Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr

595 600 605

Ser Gln Thr Tyr Phe Gly Ser Thr Ala Lys Lys Ser Pro Thr Ser Phe

610 615 620

Cys Cys Glu Trp Gly Gly Gln Ala His Arg Gln Arg Asn Thr Gly Met

625 630 635 640

Glu Ile Leu Leu Trp Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys

645 650 655

Ile Cys Leu Gly Glu Met Gln Leu Cys Thr Tyr Thr Pro His Ile Cys

660 665 670

Arg His Thr Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser

675 680 685

Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln

690 695 700

Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly Pro

705 710 715 720

Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg

725 730 735

Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe

740 745 750

Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val Thr Val Met Ala

755 760 765

Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly

770 775 780

Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg

785 790 795 800

376

Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Thr Ser Arg Ile Leu Asn

805 810 815

Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys Leu Tyr His

820 825 830

Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu Glu Lys Ala Leu

835 840 845

Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Gln Arg Pro Pro

850 855 860

Leu Gln Pro Val Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe

865 870 875 880

Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys Met

885 890 895

Leu Asn Gln Lys Cys Leu Ala Pro Phe Ile Thr His Pro Phe Ala Ser

900 905 910

Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile Arg Leu Leu

915 920 925

Lys Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser Asn Gln Leu

930 935 940

Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys Gln Gly Glu Arg

945 950 955 960

Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro Asn Phe Phe Trp Ser

965 970 975

Leu Gly Ile Gly His Thr Gly Ala Trp Trp His Thr Gly Arg Arg Arg

980 985 990

Arg Glu Trp Gly Gly Ser Thr Val Ser Arg Ser Pro Leu Trp Ile Ser

995 1000 1005

Ser Met Ile Tyr Leu Arg Gly Met Ala Phe Ala Ser Asp Ser Ser

1010 1015 1020

Ile Arg Thr Thr Arg Val Ser Trp Cys Arg Arg Cys Gly Ala Lys

1025 1030 1035

Ser Ala Ala Ala Ser Ser Leu Lys Lys Lys Pro Arg Asp Ile Cys

1040 1045 1050

Arg Ile Ser Asp Ser Lys Lys Met Thr Gln Lys Arg Glu Ser Thr

1055 1060 1065

Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Asp Lys Lys

1070 1075 1080

377

Lys Lys Ser Thr Glu Lys Thr Lys Ile Ile Lys Lys Met Lys Lys

1085 1090 1095

Arg Ile Met Val His Met Cys Ser Ser Leu Ala Leu Pro Thr Gly

1100 1105 1110

Leu Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu Ile

1115 1120 1125

Ser Ser Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro

1130 1135 1140

Ser Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp

1145 1150 1155

Thr Trp Ser Ser Trp Leu Lys Trp Leu Lys Asn Trp Lys Arg Phe

1160 1165 1170

Lys Leu Lys Lys Lys Tyr Ile Leu Ala Met Thr Leu Thr Leu Asn

1175 1180 1185

Gly His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu

1190 1195 1200

Lys Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu

1205 1210 1215

Pro Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly

1220 1225 1230

Thr Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg

1235 1240 1245

Ser Leu Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His His

1250 1255 1260

Cys Arg Thr Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr

1265 1270 1275

Gln Gly Gln Trp Ser Gly Ala Val Val Gly Leu Arg Pro Val Ala

1280 1285 1290

His His Leu Pro Arg Pro Ile Gln Gly Leu His Cys Pro Gln Ala

1295 1300 1305

Pro His Gln Pro Ala Leu Ser Trp Gly Lys Trp Pro Val Gly Met

1310 1315 1320

Thr Gly Pro Ala Arg Cys Cys Arg Trp Pro Arg Arg Glu Leu Lys

1325 1330 1335

Lys Asn Leu Leu Leu Pro Cys Arg Cys Arg Arg Glu Tyr Arg Val

1340 1345 1350

Thr Met Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala

378

1355 1360 1365

Pro Gln Val Leu Lys Lys Asn Arg Lys Gln Asn Lys Ile Ile Cys

1370 1375 1380

Lys Gly His Arg Ile Thr Ser Leu Ile Leu Ser Phe Gln Arg Val

1385 1390 1395

Lys Leu Lys Lys Lys Val Asn Ala Cys Ser Pro Ala Cys Thr Ser

1400 1405 1410

Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg Ala Val Arg Arg

1415 1420 1425

Lys Gly Ile Ile Cys Ile Lys Asn Met Phe Leu Gln Arg Lys Gly

1430 1435 1440

Phe Leu Asn Leu Ser His Val Glu Lys Phe Val Pro Pro Ser Pro

1445 1450 1455

Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys Thr Gln Leu Ala

1460 1465 1470

Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile Leu Gly Pro

1475 1480 1485

Gln Arg His Thr Pro Cys Gln Lys Lys Val Pro Ser Ala Ser Pro

1490 1495 1500

Trp Gly Leu Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile

1505 1510 1515

Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp

1520 1525 1530

Ser Ser Lys Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1115

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 1A poly-FSP

<400> 1115

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

379

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Thr Glu Ile Gln Leu Thr Met Asn

225 230 235 240

Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val Ser Pro

245 250 255

Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp Ser Arg Gln

260 265 270

Val Leu Ala Leu Ala Lys Lys Thr Leu Thr Asn Ala Ser Arg Ala Gly

275 280 285

Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly Met Ser Leu Gln Trp

290 295 300

Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys Pro Ser Arg Ser Pro

305 310 315 320

Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp His Arg Ser Gly Leu Arg

325 330 335

Ala Gln Met Pro Ser Ser Trp Ser Ser Ser Gly Arg Thr Leu Lys Leu

380

340 345 350

Ala Pro Leu Gln Val Thr Thr Thr Leu Lys Leu Ile Leu Val Met Leu

355 360 365

Glu Ile Val Thr Glu Arg Ser Leu Leu Tyr Ser Arg Met Cys Leu Val

370 375 380

Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr Phe Val Ser Leu Pro

385 390 395 400

Met Met Phe Ser Trp Ala Thr Gln Ser Phe Pro Val Gly Ala Val Phe

405 410 415

Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys Thr Ser His Ser Asn

420 425 430

Pro Trp Ser Gln Ser Asn Gln Gln Leu Lys Trp Thr Pro His Ile Leu

435 440 445

Lys Ser Ala Ser Phe Asp Lys Lys Gly Phe Val Ile Leu Ser Arg Arg

450 455 460

Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile Ala Ser Ser Pro Gly

465 470 475 480

His Pro Leu Gly Pro Thr Leu Ala Ile Arg Phe Ile Ser Ala Pro Gln

485 490 495

Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser Gly Gln Tyr Phe Ile

500 505 510

Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn Asn Met Gly Gly Asn Arg

515 520 525

Ser Ile Cys Leu Thr Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys

530 535 540

Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser

545 550 555 560

Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe Thr Lys Cys Glu Ile

565 570 575

Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile Thr Pro Gln

580 585 590

Asp Pro His Leu Asp Pro Val Cys His Pro His Arg Arg Pro Gly Trp

595 600 605

Lys Gly His His Arg Val Val Phe Ser His His Pro Ser Gly Ser Thr

610 615 620

Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg Arg Glu Lys

625 630 635 640

381

Lys Gly Cys Arg Glu Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro

645 650 655

Val Pro Leu Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg

660 665 670

Ser Cys Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met

675 680 685

Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg Met Trp Trp Lys

690 695 700

Gly Lys Lys Arg Arg Lys Glu Ile Val Pro Met Ser Leu Ala Cys Ser

705 710 715 720

Ile Thr Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val

725 730 735

Ser Ser Ile Ser His Trp Arg Leu Pro Cys Lys Val Thr Glu Ser Ser

740 745 750

Ser Ser Phe Phe Tyr Thr Ser Asp Arg Thr Pro Leu Ser Ile Ser Arg

755 760 765

Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu Thr His

770 775 780

Pro Ala His Gln Pro Leu Gly Ser Met Ser Thr Arg Arg Glu Met Pro

785 790 795 800

Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp Arg Ala Ala Leu Gly

805 810 815

Lys Glu Val Lys Arg Ser Gln Ser Lys Leu Arg Leu Arg Asn Thr Ser

820 825 830

Pro Gly Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser Asp Thr Thr

835 840 845

Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys Gly Asn Leu

850 855 860

His Leu Lys Thr Thr Ser Leu Gln Arg Pro Pro Arg Ser Ile Leu Ser

865 870 875 880

Leu Asn Gly Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys Ile

885 890 895

Ser Val Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile Thr

900 905 910

Glu Cys Val Arg Thr Ala Leu Asn Val Ser Lys Lys Arg Leu Pro Lys

915 920 925

382

Leu Met Lys Lys Lys Asp Ser Thr Ile Phe Ile Asn Lys Val Arg Gln

930 935 940

Asn Phe Lys Arg Asn Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser Pro

945 950 955 960

Ser Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met Met Met

965 970 975

Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met Thr Met

980 985 990

Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro Arg

995 1000 1005

Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln

1010 1015 1020

Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp

1025 1030 1035

Gly Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly Thr

1040 1045 1050

Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr Cys Pro

1055 1060 1065

Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala Pro

1070 1075 1080

Val Gly Ala Gln Ala Pro Pro Glu Val Thr Leu Arg Ala Ser Cys

1085 1090 1095

Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp Gly

1100 1105 1110

Ala Gln Val Arg Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys

1115 1120 1125

Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly

1130 1135 1140

Ala Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln

1145 1150 1155

Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg

1160 1165 1170

Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro

1175 1180 1185

Lys Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala

1190 1195 1200

383

Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly Met Leu

1205 1210 1215

Leu Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala Arg

1220 1225 1230

Arg Glu Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg

1235 1240 1245

Pro Pro His Leu Pro Ser Cys His Pro Leu Pro Gln Leu Leu Gly

1250 1255 1260

Pro Pro Pro Pro His Gln Ala Pro Met Thr Leu Lys Ala Leu Val

1265 1270 1275

Lys Thr Trp Thr Gln Phe Arg Asn Tyr Pro Arg Ala Asp Lys Lys

1280 1285 1290

Asn Val Arg Ser Thr Ala Phe Phe Leu Leu Asp Ile Ile Leu Gly

1295 1300 1305

Asp Cys Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro

1310 1315 1320

Ala Ser Gly Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg

1325 1330 1335

Ala Cys Arg Ala Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Tyr

1340 1345 1350

Pro Arg Leu Lys Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys

1355 1360 1365

Leu Ala Gly Ser Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg

1370 1375 1380

Gln Glu Cys Gly Arg Arg Ala Ser Val Pro Cys Arg Pro Met Ile

1385 1390 1395

Gly Ser Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser

1400 1405 1410

Ala Met Gly Val Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser

1415 1420 1425

Pro Pro Ala Thr Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met

1430 1435 1440

Met Ser Trp Met Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys

1445 1450 1455

Ser Pro Ala Pro Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro

1460 1465 1470

Thr Ala Val Pro Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro

384

1475 1480 1485

Val Leu Ser Gly Cys His Leu Ala Val Arg Thr Ser Met Leu Thr

1490 1495 1500

Leu Leu Pro Met Glu Ala Lys Lys Ser Met Lys Arg Ser Asn Ala

1505 1510 1515

Lys Lys Met Glu Asp Met Ile Trp Glu Ser Arg Leu Pro Leu Arg

1520 1525 1530

Pro Gln Arg Gly Pro Arg Pro Leu His Arg Trp Leu Gln Gln Thr

1535 1540 1545

Thr Gly Cys Ser Gln Arg Arg Ile Val Phe Phe Leu Ala Pro Tyr

1550 1555 1560

Phe Pro Arg His Pro His Arg Ser Pro Pro Thr Ser Cys Pro Pro

1565 1570 1575

Leu Leu Glu Ala Phe Pro Leu Pro Arg Ala Ser Leu Arg Ala Thr

1580 1585 1590

Val Pro Arg His Ser Ser Val Leu Ala Thr Gly Leu His Leu His

1595 1600 1605

Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe Leu Leu His Gln Pro

1610 1615 1620

Leu Pro Gly Gln His Leu Trp Leu Ser His Arg Leu Arg Leu Arg

1625 1630 1635

Leu Pro Arg Thr Gln Pro Ser Leu Pro Gly Pro Cys Ala Ser Leu

1640 1645 1650

Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro Ser Gln Val

1655 1660 1665

Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro Ala Ala Ala

1670 1675 1680

Cys Pro Pro Ala Leu Arg Ser Met Pro Lys Ser Arg Asn Gln Val

1685 1690 1695

Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu Ala

1700 1705 1710

Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser

1715 1720 1725

Arg Lys Gln Ile Pro Ser Gln Ser Met Leu Val Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

385

<210> 1116

<211> 1744

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 2A poly-FSP

<400> 1116

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Arg Lys Met Gln Ser Leu Asn His

225 230 235 240

Pro Ser Arg Leu Thr Asn Gly Ser Pro Pro Leu His Leu Cys Gln Pro

386

245 250 255

Leu Arg Met Ser Arg Thr Pro Pro Arg Arg Val Cys Ala Ala Pro Pro

260 265 270

His Arg Leu Leu Gly Pro Ser Arg Trp Asn Pro Arg Arg Lys Thr Trp

275 280 285

Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn His Leu

290 295 300

Tyr Gly Gly Lys Lys Arg Ser Lys Phe Ser Arg Ser His Leu Asp Phe

305 310 315 320

Ser Pro Tyr Trp Met Lys Lys Val Lys Val Leu Phe Lys Ala Asp Gln

325 330 335

Ser Glu Ser Ser Leu Ser Ser Gly Arg Cys Ser Leu Met Glu Gln Ile

340 345 350

Pro His Leu Lys Ser Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys

355 360 365

Asn Leu Trp Leu Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly

370 375 380

Phe Gln Trp Gly Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly

385 390 395 400

Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala

405 410 415

Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser Arg

420 425 430

Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile His His Arg

435 440 445

Lys Asn Pro Leu Leu Pro Met Arg Trp Trp Cys Thr Arg Gly Gly Gly

450 455 460

Lys Arg Lys Lys Lys Met Cys Lys Val Ser Gly Gly Cys Thr Ala Cys

465 470 475 480

Val Glu Arg Cys Pro Trp Lys Gly Ile Cys Trp Lys Trp Pro Val Thr

485 490 495

Val Ala Ala Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe Tyr Ile

500 505 510

Ser Ser Thr Val Cys Phe Leu Ser Lys His Cys Met Thr Ala Cys Thr

515 520 525

Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln

530 535 540

387

Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala

545 550 555 560

Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe

565 570 575

Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg

580 585 590

Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg

595 600 605

Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly

610 615 620

Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe

625 630 635 640

Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr Ile Leu Phe

645 650 655

His Val Arg Asn Ile Val Tyr Gly Ile Gln Val Met Leu Cys Ala Ser

660 665 670

Pro Gln Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg

675 680 685

Arg Met Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro

690 695 700

Gly Ile Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Ala Ser

705 710 715 720

Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Arg

725 730 735

Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu Ile Ala His

740 745 750

Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro Arg Leu Leu

755 760 765

Thr Arg Lys Lys Pro Gly Lys Leu Leu Leu Ala His Met Tyr Gly Phe

770 775 780

Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys Val Cys Leu Gln Cys Leu

785 790 795 800

Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln Pro Leu Asp Leu Lys Ile

805 810 815

Ser Lys Thr Phe Met Gly His Arg Glu Gly Ser Glu Ser Asp Asn Leu

820 825 830

388

Gly Leu Leu Tyr Ser Phe Pro Pro Pro Tyr Pro Ile Ser Glu Gly Leu

835 840 845

Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu Met Thr Asn

850 855 860

Cys Leu Phe Ser Pro Ile Phe Leu Cys His His Ala Ile Met His Arg

865 870 875 880

Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser Ser Lys Ile Pro His

885 890 895

Pro Gln Val Pro Ser Val Val Ala Leu Cys Lys Phe Arg Ala Phe Ile

900 905 910

His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr Ala Asn Met Cys Trp

915 920 925

Pro Lys Phe Phe Leu Thr Phe Ser Gln Gln Trp Ser Ser Val Thr Ser

930 935 940

Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys Ser Ser Trp Leu Met Lys

945 950 955 960

Gln Thr Trp Met Lys Ser Trp Ser Leu Arg Asp Pro Ser Tyr Arg Ser

965 970 975

Ile Leu Glu Tyr Val Ser Thr Arg Val Leu Trp Met Pro Thr Ser Thr

980 985 990

Val Leu Gln Ala His Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp

995 1000 1005

Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln

1010 1015 1020

Gln Ser Leu Thr Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser Cys

1025 1030 1035

Pro Phe Gln Ser Pro Thr Lys Arg Leu Ile Tyr Pro Pro Ser Thr

1040 1045 1050

Cys Ser Leu Pro Cys Arg Gly Phe Leu Pro Ser Arg Phe Pro Trp

1055 1060 1065

Gly Pro Pro Pro Leu Asn Ser Val Ile Val His Lys Ile Val Leu

1070 1075 1080

Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu Arg Arg Ile

1085 1090 1095

Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu Lys Leu

1100 1105 1110

389

Leu Asn Met Asp Leu Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr

1115 1120 1125

Ser Pro Leu Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn Cys

1130 1135 1140

Val Val His Val Leu Met Phe Gly Ala Leu Arg Ala Thr Ala Pro

1145 1150 1155

Ala Thr Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg Ala Ala

1160 1165 1170

Thr Thr Ser Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr Ser

1175 1180 1185

Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser

1190 1195 1200

Cys Tyr Trp Ser Leu Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu

1205 1210 1215

Ala Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly Arg

1220 1225 1230

Met Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys

1235 1240 1245

Phe Thr Gly Val Arg Thr Ser Ser Arg Tyr Lys Lys Arg Gly Val

1250 1255 1260

Ile Ala Trp Arg Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg Lys

1265 1270 1275

Lys His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr

1280 1285 1290

Cys Ile Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys Gln Ser

1295 1300 1305

Gln Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met

1310 1315 1320

Trp Thr Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr Leu His Gln

1325 1330 1335

Gln Lys Thr Gln Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp

1340 1345 1350

Leu His Thr Gln Cys Gln Glu Lys Lys Ser Arg Lys Leu Met Ser

1355 1360 1365

Ser Cys Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly Cys His

1370 1375 1380

Pro Ala Trp Leu Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro

390

1385 1390 1395

Tyr Pro Phe His Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly

1400 1405 1410

Thr Ala Met Pro Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu

1415 1420 1425

Lys Gly Trp Asp Lys Pro Gly Thr Ser Leu Gln Cys Tyr Leu Asn

1430 1435 1440

Arg Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln Leu Ala Ile

1445 1450 1455

Gln Val Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe Gly Gln

1460 1465 1470

Lys Lys Gly His Met Ile Phe Ser Leu Ala Asn Gly Leu Trp Phe

1475 1480 1485

Asn Leu Ile Phe Asn Ser Arg Lys Lys Leu Leu Glu Asp Met Lys

1490 1495 1500

Gln Asn Gly Phe Thr Gly Lys Gly Leu Thr His Pro Val Pro Leu

1505 1510 1515

Lys Arg Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val

1520 1525 1530

Asn Gln Lys Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His

1535 1540 1545

Leu Ile Val Leu Asn Ser Pro Glu Ser Asn Leu Ile Leu Ile Gln

1550 1555 1560

His Gly Gly Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu Ser

1565 1570 1575

Trp Asn Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val

1580 1585 1590

Gln Arg Leu Leu Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val

1595 1600 1605

Leu Asn Leu Phe His Val Pro Val Asn Gln Ile Phe Met Met Ala

1610 1615 1620

Phe Tyr Leu Lys Tyr Thr Glu Lys Ala Trp Val Met Glu Leu Glu

1625 1630 1635

Trp Leu Ile Gln Gln Gln Cys Tyr Gln Gly Ser Cys Gly Arg Ala

1640 1645 1650

Cys Ser Cys Gly Arg Gly Cys Gly Thr Pro Arg Gly Asp Trp Lys

1655 1660 1665

391

Arg His Pro Pro Arg Arg Ala Phe Trp Lys Pro Pro Ser Gln Gly

1670 1675 1680

Ile Ser Ser Cys Leu Met Thr Gly Cys Trp Leu Glu Lys Lys Ala

1685 1690 1695

Thr Trp Met Arg Arg Thr Arg Arg Lys Lys Arg Glu Asp His Cys

1700 1705 1710

Gly Leu Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met

1715 1720 1725

Arg Pro Pro Arg Ser Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala

1730 1735 1740

Ser

<210> 1117

<211> 1749

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 3A poly-FSP

<400> 1117

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

392

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Gly Ala Ala Ala Ala Ser Cys Gln

225 230 235 240

Ser Pro Pro Ser Pro Arg Glu Thr Thr Pro Ser Ser Asp Ala Thr Arg

245 250 255

Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys Thr Met Thr Ala Ala

260 265 270

Gly Leu Thr Pro Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala

275 280 285

Asn Arg Cys Leu Arg Val Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr

290 295 300

Pro Arg Gly Gln Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser

305 310 315 320

Leu Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu Ser Ser Leu Phe Phe

325 330 335

Ser His Cys Leu Cys Arg Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys

340 345 350

Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Pro Ile Pro Pro His Leu

355 360 365

Arg His Pro Leu Leu Leu Met Asp Leu Asp Gly Met Leu Thr Val Ile

370 375 380

Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu Gly Thr Val Ala

385 390 395 400

Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln Val Gln Thr Gly Gly

405 410 415

Arg Leu Thr Pro Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro Val

420 425 430

Asp Pro Pro Gly Ser Val Trp Ile Leu Pro Cys Lys Asn Lys Lys Arg

435 440 445

393

Arg Lys Leu Arg Pro Lys Arg Leu Asp Phe His Pro Arg Ser Ser Ser

450 455 460

Gly Val Ser Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly

465 470 475 480

Leu Asn Trp Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met Asn

485 490 495

Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala Arg Lys Ser Gly Val Met

500 505 510

Lys Cys Trp Asp Leu Cys Pro Ser Glu Lys Lys Arg Lys Leu Pro Leu

515 520 525

Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His Arg Gly Ala

530 535 540

Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe Phe Thr Ser Ala His

545 550 555 560

Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln Phe Leu Ile His Leu

565 570 575

Arg Lys Val Asp Thr His His Leu Gln Val Val Thr Lys Thr Val Thr

580 585 590

Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys Lys Ala

595 600 605

Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro

610 615 620

Lys Ser His Phe Pro Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser

625 630 635 640

Leu Gln Leu Arg Ser Ile Pro His Val Pro Lys Ala Thr Ala Ala His

645 650 655

Ser Val Thr Val Lys Leu Gln Lys Gly Lys Asn Ser Pro Cys Cys Gln

660 665 670

Lys Lys Leu Arg Val Gln Asn Gln Gly His Leu Leu Met Ile Leu Leu

675 680 685

His Asn Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val

690 695 700

Ala Met Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu Ser

705 710 715 720

Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro Val Thr

725 730 735

394

Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro Gln Thr Ala

740 745 750

Ala Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro Thr Thr Arg Leu

755 760 765

Met Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu Leu Thr Gln Pro

770 775 780

Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu His Pro Gln Trp

785 790 795 800

Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys

805 810 815

Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr Trp Gln Val Ser

820 825 830

Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser Arg Arg Gly Met Lys Ser

835 840 845

Ser Val Arg Leu Lys Asn Ser Arg Arg Asn Arg Arg Tyr Arg Lys Ile

850 855 860

Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Val Ala Pro Arg Cys Pro

865 870 875 880

Arg Ser Ala Val Asp Ile Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala

885 890 895

Ser Cys Ala Ser Val Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser

900 905 910

Ala Pro Cys Gly Ala Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val

915 920 925

Leu Gly Thr Met Val Met Lys His Leu Asn Arg Lys Leu His Leu Thr

930 935 940

Arg Ser Cys Leu Ile Asp Leu Thr Met Met Met Ser Gln Lys Leu Trp

945 950 955 960

Lys Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro Arg Gln His Leu

965 970 975

Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro Cys Leu Leu Leu Leu

980 985 990

His His Arg His His Leu Ala Phe Leu Glu Met Ala Cys Ser Ser Gln

995 1000 1005

His Thr His Ser Ile Lys Ile Trp Ile Ser Phe Ser His Glu Gln

1010 1015 1020

395

Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn

1025 1030 1035

Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His His Cys

1040 1045 1050

Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp Lys

1055 1060 1065

Lys Met Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp

1070 1075 1080

Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly Lys Arg Cys

1085 1090 1095

Asn Pro Cys Cys Arg Leu Thr Trp Met Ser Met Leu Gln Lys Gln

1100 1105 1110

Cys Cys Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile

1115 1120 1125

Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser

1130 1135 1140

Leu Gly Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe

1145 1150 1155

Ile Cys Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr

1160 1165 1170

Lys Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly

1175 1180 1185

Lys Tyr Lys Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala

1190 1195 1200

Met Thr Val Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe Met

1205 1210 1215

Arg Cys Val Gln Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro

1220 1225 1230

Leu Gly Arg Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn Val

1235 1240 1245

Ser Ser Val Leu Lys Pro Phe Leu Phe Thr Val Pro Ile Glu Asp

1250 1255 1260

Met Arg Glu Cys Thr Leu Gly Arg Asn Arg Met Asn Val Ser Ser

1265 1270 1275

Val Leu Lys Pro Cys Leu Ile Pro Val Pro Ile Asp Leu Pro Arg

1280 1285 1290

Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln Met Pro Phe

396

1295 1300 1305

Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr

1310 1315 1320

Pro Ser Thr Trp Gly Trp Arg Thr Phe Arg Gly Pro Arg Arg Lys

1325 1330 1335

Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln Ser Leu Gln Lys

1340 1345 1350

Ser Gln Val Phe Gln Trp Val Leu Arg Thr Glu Asn Ile Arg Lys

1355 1360 1365

Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe Pro Lys

1370 1375 1380

Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys Ala Phe Ser

1385 1390 1395

Gly Arg Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met

1400 1405 1410

Pro Lys Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr Cys Thr Asn

1415 1420 1425

Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val

1430 1435 1440

Phe Ile Leu Leu Leu Ser Asp Lys Lys Leu Leu Lys Lys Ser Ser

1445 1450 1455

Gln Leu Arg Arg Ser Trp Arg Asn Val Ser Arg Ile Ser His Gln

1460 1465 1470

Met Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe Trp Asp

1475 1480 1485

Met Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu

1490 1495 1500

Thr Lys Arg Glu Gly Pro Glu Thr Cys Lys Lys Lys Leu Leu Leu

1505 1510 1515

Lys Leu Leu Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg

1520 1525 1530

Leu Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln His Leu

1535 1540 1545

Lys Lys Thr Ser Ala Lys Gly Glu Lys Leu Lys His Cys Phe Lys

1550 1555 1560

Ala Trp Gly Arg Gly Pro Pro Leu His Thr Arg Ser Gln Lys Lys

1565 1570 1575

397

Asp Pro Ala Gly Lys Leu Lys Lys Leu Thr Thr Leu Ser Phe Val

1580 1585 1590

Leu Ile Glu Glu Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu

1595 1600 1605

Trp Leu Lys Lys Ser Thr Lys Ser Arg Tyr Leu Phe Phe Leu Thr

1610 1615 1620

Val Cys Gly Phe Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser Ser

1625 1630 1635

Arg Lys Lys Gly His Thr Lys Asn Arg Arg Lys Ala Lys Arg Lys

1640 1645 1650

Pro Gln Ile Val His Lys Lys Arg Gly Leu Phe Leu Lys Lys Lys

1655 1660 1665

Tyr Leu Val Gln Gly Ala Lys Lys Ile Thr Phe Leu Ser Asn Gly

1670 1675 1680

Met Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu Ser Asp Arg

1685 1690 1695

Val Phe Leu Val Lys Ile Thr Thr Thr Thr Ser Ser Pro Arg Arg

1700 1705 1710

Ser Pro Ala Leu Arg Ala Arg Gly Gly Thr Thr Ile Gly Glu Val

1715 1720 1725

Thr Ser Leu Cys Phe Phe Tyr Leu Leu Tyr Gly Tyr Pro Tyr Asp

1730 1735 1740

Val Pro Asp Tyr Ala Ser

1745

<210> 1118

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 4A poly-FSP

<400> 1118

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

398

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Ile Gln Lys Lys Lys Gln Lys Ser

225 230 235 240

Met Ala Lys Lys Ser Gln Gln Pro Leu Val Leu Lys Lys Asn Arg Lys

245 250 255

Gln Glu Trp Gly Gly Gln Ala His Arg Gln Arg Asn Asn Ala Lys Lys

260 265 270

Met Pro Phe Gln Glu Glu Lys Lys Asn Gly Arg Ile Ser Ser Ser Leu

275 280 285

Ile Leu Ser Phe Gln Arg Val Lys Asp Ser Lys Lys Met Thr Gln Lys

290 295 300

Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu

305 310 315 320

Pro Ser Ser Asp Leu Ala Val Leu Ile Ser Ser Ser Thr Ser His Phe

325 330 335

Leu Met Arg Ser Pro Val Leu Pro Ser Ser Arg Leu Thr Cys Ala Ser

340 345 350

399

Pro Gln Leu Pro Arg Met Trp Thr Trp Ser Ser Ser Trp Leu Lys His Pro

355 360 365

Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly Ser Arg

370 375 380

Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr Lys Trp

385 390 395 400

Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr Leu Val

405 410 415

Trp Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala His Cys Pro

420 425 430

Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser Ser Cys

435 440 445

Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala Val Val

450 455 460

Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile Gln Gly Leu

465 470 475 480

His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser Trp Gly Lys Trp

485 490 495

Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys Arg Trp Pro Arg Arg

500 505 510

Ser Gln Lys Ser Gln His His Cys Ala Leu Arg Lys Ile Glu Trp Ala

515 520 525

Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys

530 535 540

Ala Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro

545 550 555 560

Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp Ser Ser Ser Lys Cys

565 570 575

Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser Cys Ser Pro Ser

580 585 590

Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln Gly Cys Leu Arg

595 600 605

Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly Pro Gly Ser Pro Ala

610 615 620

Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg Trp Trp Gly Pro

625 630 635 640

400

Gly Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe Pro Pro Pro Asp

645 650 655

Pro Pro Trp Pro Val Thr Ala Val Thr Val Met Ala Gly Ser Val Pro

660 665 670

Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly Pro Leu Ala Leu

675 680 685

Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg Glu Met Ser Ile

690 695 700

Phe Leu Pro Gly Ile Phe Cys Gln Lys Lys Val Pro Ser Ala Ser Pro

705 710 715 720

Trp Gly Leu Val Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg Met

725 730 735

Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro Val

740 745 750

Trp His Ile Leu Gly Pro Gln Arg His Thr Pro Gln Val Val Leu Arg

755 760 765

Leu Gly Asn Leu Pro Pro Phe Phe Arg Gly Ser Glu Val Pro Phe Pro

770 775 780

Arg Pro Cys Arg Thr Lys Asn Glu Gly Asn Asn His Trp Pro Arg Val

785 790 795 800

Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr

805 810 815

Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys His Trp Gln Gln Cys

820 825 830

Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe

835 840 845

His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro Gly Trp

850 855 860

Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala

865 870 875 880

Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp Leu Ile

885 890 895

Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly Phe Cys His Val Ala

900 905 910

Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr Asp Val

915 920 925

401

Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr Gly Met

930 935 940

Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala Arg Leu

945 950 955 960

Leu Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile Ser Asn Arg Lys Thr

965 970 975

Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp Arg Ala Ser Val

980 985 990

Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly Val Arg Thr Arg Gly Ser

995 1000 1005

Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg Cys Arg Cys Arg Arg

1010 1015 1020

Glu Tyr Arg Val Thr Met Asn Ile Leu Asn Ser Leu Pro Ser Ser

1025 1030 1035

Met Glu Ile Ala Val Leu Gln Glu Thr Ala Lys Lys Ser Pro Thr

1040 1045 1050

Ser Phe Cys Cys Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys

1055 1060 1065

Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro Lys Ala

1070 1075 1080

Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn Leu

1085 1090 1095

Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys

1100 1105 1110

Asp Glu Glu Asp His Gln Lys Arg His His His His Asn Gln Lys

1115 1120 1125

Lys Met Ser Val Pro Leu Phe Phe His Gly Asp Leu Cys Arg Glu

1130 1135 1140

Ser Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Gln

1145 1150 1155

Arg Pro Pro Leu Gln Pro Val Thr Gly Met Glu Ile Leu Leu Trp

1160 1165 1170

Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly

1175 1180 1185

Glu Met Gln Leu Cys Thr Ser Arg Ile Leu Asn Leu Gln Val Leu

1190 1195 1200

Lys Lys Ile Leu Arg Ser Phe Met Lys Leu Tyr His Ser Leu Val

402

1205 1210 1215

Met Cys Leu Arg Leu Arg Thr Lys Leu Glu Lys Ala Leu Ser Ala

1220 1225 1230

Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Ile Thr His Pro Phe

1235 1240 1245

Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile

1250 1255 1260

Arg Leu Leu Lys Pro Asp Lys Lys Lys Lys Ser Thr Glu Lys Thr

1265 1270 1275

Lys Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala Pro

1280 1285 1290

Gln Gly Gln Ala Phe Gly Ser Leu Cys Gln Leu Val Ser Ala Asp

1295 1300 1305

Phe Ser Ala Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His Leu

1310 1315 1320

Leu Ile Trp Leu Lys Asn Trp Lys Arg Phe Lys Leu Lys Lys Lys

1325 1330 1335

Tyr Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln

1340 1345 1350

Thr Tyr Phe Gly Ser Ile Ile Cys Ile Lys Asn Met Phe Leu Gln

1355 1360 1365

Arg Lys Gly Phe Leu Asn Leu Ser His Val Glu Lys Phe Glu Leu

1370 1375 1380

Lys Lys Asn Leu Leu Leu Pro Val Val Ile Asn Leu Lys Arg Leu

1385 1390 1395

Gln Lys Phe Met Leu Asn Gln Lys Cys Leu Ala Pro Phe Pro Ala

1400 1405 1410

Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg Ala

1415 1420 1425

Val Arg Arg Lys Gly Ile Leu Ala Met Thr Leu Thr Leu Asn Gly

1430 1435 1440

Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile

1445 1450 1455

Ser Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu Ala Ile Ile

1460 1465 1470

Lys Lys Met Lys Lys Arg Ile Met Val His Arg Trp Ile Phe Ser

1475 1480 1485

403

Asn Gln Leu Thr Val Gln Met Leu Cys Leu Pro Leu Leu Thr Pro

1490 1495 1500

Asp Thr Arg Trp Ile Phe Ser Asn Gln Leu Gln Thr Val Gln Met

1505 1510 1515

Leu Cys Leu Pro Pro Leu Lys Gln Gly Glu Arg Ile Ile Trp Pro

1520 1525 1530

Leu Gly Gly Phe Gln Ile Pro Asn Phe Phe Trp Ser Leu Gly Ile

1535 1540 1545

Gly His Thr Gly Ala Trp Trp His Thr Gly Arg Arg Arg Arg Glu

1550 1555 1560

Trp Gly Gly Ser Thr Val Ser Arg Ser Pro Leu Trp Ile Ser Ser

1565 1570 1575

Met Ile Tyr Leu Arg Gly Met Ala Phe Ala Ser Asp Ser Ser Ile

1580 1585 1590

Arg Thr Thr Arg Val Ser Trp Cys Arg Arg Cys Gly Ala Lys Ser

1595 1600 1605

Ala Ala Ala Ser Ser Ser Pro Leu Trp Asp Cys Ser Thr Thr Thr

1610 1615 1620

Gly Thr His His Gly Cys Leu Trp Glu Ile Pro Ser Val Thr Leu

1625 1630 1635

Leu Ala Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg

1640 1645 1650

Asn Lys Ser Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln Leu

1655 1660 1665

Lys Lys Lys Val Asn Ala Cys Ser Thr Tyr Thr Pro His Ile Cys

1670 1675 1680

Arg His Thr Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg

1685 1690 1695

Arg Met Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr

1700 1705 1710

Val Arg Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp

1715 1720 1725

Asn Lys Ile Ile Cys Lys Gly His Arg Ile Thr Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

404

<210> 1119

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 1B poly-FSP

<400> 1119

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Thr Glu Ile Gln Leu Thr Met Asn

225 230 235 240

Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val Ser Pro

245 250 255

405

Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp Ser Arg Gln

260 265 270

Val Leu Ala Met Pro Ser Ser Trp Ser Ser Ser Gly Arg Thr Gln Arg

275 280 285

Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro Leu Arg Asn Asp Gly

290 295 300

Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser Leu Lys

305 310 315 320

Lys Ser Leu Glu Lys Met Pro Leu Met Met Lys Lys Lys Asp Ser Thr

325 330 335

Ile Phe Ile Val Pro Met Ser Leu Ala Cys Ser Ile Thr Val Ser Phe

340 345 350

Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val Ser Ser Ile Ser His

355 360 365

Trp Asn Lys Val Arg Gln Asn Phe Lys Arg Asn Ala Asp Lys Lys Asn

370 375 380

Val Arg Ser Gly Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser Asp

385 390 395 400

Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys Gly

405 410 415

Asn Leu His Leu Lys Thr Thr Ser Leu Gly Asp Cys Pro Arg Thr Met

420 425 430

Ala Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser Arg

435 440 445

Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro Ala

450 455 460

Gly Trp Ala Arg Gln Ala Phe Asp Lys Lys Gly Phe Val Ile Leu Ser

465 470 475 480

Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys Val Pro Val Ala Glu

485 490 495

Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala Ala Gly Thr Arg Cys

500 505 510

Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro Ala Ser Ser Pro Thr

515 520 525

Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg Arg Lys Arg Ser Ser

530 535 540

406

Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu Arg Met Met Leu Pro

545 550 555 560

Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro

565 570 575

Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg

580 585 590

Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu

595 600 605

Gly Leu Arg Pro Pro His Leu Pro Ser Cys His Pro Leu Pro Gln Leu

610 615 620

Leu Gly Pro Pro Pro Pro His Gln Ala Pro Thr Ala Phe Phe Leu Leu

625 630 635 640

Asp Ile Ile Leu Arg Leu Pro Cys Lys Val Thr Glu Ser Ser Asn Met

645 650 655

Gly Gly Asn Arg Ser Ile Cys Leu Thr Asn Ala Lys Lys Met Glu Asp

660 665 670

Met Ile Trp Glu Ser Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg

675 680 685

Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu Thr His Pro Ala His Gln

690 695 700

Pro Leu Gly Ser Met Thr Leu Ala Ile Arg Phe Ile Ser Ala Pro Gln

705 710 715 720

Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser Gly Gln Tyr Phe Lys

725 730 735

Gly Lys Lys Arg Arg Lys Glu Ile Phe Lys Lys Lys Lys Pro Leu Thr

740 745 750

Phe Ser Pro Ser Leu Ala Lys Lys Thr Leu Thr Asn Ser Ser Phe Phe

755 760 765

Tyr Thr Ser Asp Arg Glu Ala Lys Lys Ser Met Lys Arg Ser Met Thr

770 775 780

Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe Arg Asn Tyr Pro Arg

785 790 795 800

Ala Ser Ser Pro Gly His Pro Leu Gly Pro Asn Gly Ile Trp Glu Val

805 810 815

Met Pro Arg Thr Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr

820 825 830

407

Ser Gln Asn His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu

835 840 845

Asn Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile Ala Leu

850 855 860

Arg Ser Met Pro Lys Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu

865 870 875 880

Thr Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro

885 890 895

Gln Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln Ser

900 905 910

Met Leu Val Ala Ser Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr

915 920 925

Ser Arg Gly Met Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro

930 935 940

Leu Arg Cys Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn

945 950 955 960

Gly Trp His Arg Ser Gly Leu Arg Ala Gln Arg Lys Arg Lys Arg Glu

965 970 975

Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys

980 985 990

Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe

995 1000 1005

Val Ser Lys Lys Arg Leu Pro Lys Leu Thr Lys Cys Glu Ile Cys

1010 1015 1020

Val Lys Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile Thr Pro Gln

1025 1030 1035

Asp Pro His Leu Asp Pro Val Cys His Pro His Arg Arg Pro Gly

1040 1045 1050

Trp Lys Gly His His Arg Val Val Phe Ser His His Pro Ser Gly

1055 1060 1065

Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg

1070 1075 1080

Arg Glu Lys Lys Gly Cys Arg Glu Arg Ser Leu Leu Tyr Ser Arg

1085 1090 1095

Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr

1100 1105 1110

Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe

408

1115 1120 1125

Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys

1130 1135 1140

Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Arg His Pro

1145 1150 1155

His Arg Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe

1160 1165 1170

Pro Leu Pro Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His Ser

1175 1180 1185

Ser Val Leu Ala Thr Gly Leu His Leu His Arg Gln Ile Ser Leu

1190 1195 1200

Pro Leu Arg Gly Phe Leu Leu His Gln Pro Leu Pro Gly Gln His

1205 1210 1215

Leu Trp Leu Ser His Arg Leu Arg Leu Arg Leu Pro Arg Thr Gln

1220 1225 1230

Arg Pro Pro Arg Ser Ile Leu Ser Leu Arg Leu Pro Leu Arg Pro

1235 1240 1245

Gln Arg Gly Pro Arg Pro Leu His Arg Trp Leu Gln Gln Thr Thr

1250 1255 1260

Gly Cys Ser Gln Arg Arg Tyr Pro Arg Leu Lys Thr Phe Gly Val

1265 1270 1275

Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr Met

1280 1285 1290

Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser

1295 1300 1305

Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro Trp

1310 1315 1320

Arg Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro Thr

1325 1330 1335

Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg Met

1340 1345 1350

Pro Gly Ser Gln Ser Ile Met Ser Trp Met Pro Pro Leu Ala

1355 1360 1365

Pro Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys Pro

1370 1375 1380

Ala His Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr Pro

1385 1390 1395

409

Leu Ser Ala His Pro Val Pro Val Leu Ser Gly Cys His Leu Ala

1400 1405 1410

Val Arg Thr Ser Met Leu Thr Leu Leu Pro Met Leu Lys Leu Ala

1415 1420 1425

Pro Leu Gln Val Thr Thr Thr Leu Lys Leu Ile Leu Val Met Leu

1430 1435 1440

Glu Ile Val Thr Glu Ser Thr Arg Arg Glu Met Pro Ser Gln Ala

1445 1450 1455

Thr Pro Ser Pro Pro Gly Pro Trp Arg Ala Ala Leu Gly Lys Glu

1460 1465 1470

Val Lys Arg Ser Gln Ser Lys Leu Arg Leu Arg Asn Thr Ser Pro

1475 1480 1485

Ile Val Phe Phe Leu Ala Pro Tyr Phe Pro Arg Arg Met Thr Arg

1490 1495 1500

Arg Thr Met Thr Thr Thr Lys Met Met Met Met Arg Arg Thr Lys

1505 1510 1515

Arg Arg Arg Arg Lys Arg Arg Arg Met Thr Met Met Thr Arg Arg

1520 1525 1530

Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro Arg Thr Ser Ala

1535 1540 1545

Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln Arg Arg Pro

1550 1555 1560

Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp Gly Asn Gln

1565 1570 1575

Gln Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser Arg Arg

1580 1585 1590

Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg Met Trp Trp Ile Ala

1595 1600 1605

Lys Lys Arg Ile Lys Leu Arg Arg Asn Arg Thr Arg Leu Thr Leu

1610 1615 1620

Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser Ala Val

1625 1630 1635

Cys Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala Ala

1640 1645 1650

Ser Pro Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro Pro

1655 1660 1665

410

Glu Val Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala

1670 1675 1680

Cys Ala Gly Leu Trp Pro Trp Gly Ala Gln Val Arg Gln Pro Ser

1685 1690 1695

Leu Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro

1700 1705 1710

Pro Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala Thr Leu Arg

1715 1720 1725

Cys Pro Ala Val Pro Ala Ala Ala Cys Pro Pro Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1120

<211> 1744

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 2B poly-FSP

<400> 1120

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

411

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Arg Lys Met Gln Ser Leu Asn His

225 230 235 240

Pro Ser Arg Leu Thr Asn Met Ser Arg Thr Pro Pro Arg Arg Val Cys

245 250 255

Asn Pro Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys

260 265 270

Pro Ser Gln Lys Asn His Leu Tyr Asn Ser Arg Lys Lys Leu Leu Glu

275 280 285

Asp Met Lys Gln Asn Gly Phe Thr Gly His Arg Glu Gly Ser Glu Ser

290 295 300

Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Ile Arg Met Cys Ala

305 310 315 320

Leu Ser His Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser

325 330 335

Lys His Cys Met Thr Ala Cys Thr Gln Lys Lys Gly His Met Ile Phe

340 345 350

Ser Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser Cys Pro Phe Gln Ser

355 360 365

Pro Thr Lys Arg Leu Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr Leu

370 375 380

His Gln Gln Lys Thr Gln Gly Gly Lys Lys Arg Ser Lys Phe Pro Gly

385 390 395 400

Thr Ser Leu Gln Cys Tyr Leu Asn Arg Cys Ser Leu Met Glu Gln Ile

405 410 415

Pro His Leu Lys Ser Ala Ser Pro Gln Ile Met Gln Arg Thr Ser Ala

420 425 430

Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn Leu Asp Asn Leu Gly

435 440 445

412

Val Pro Trp Pro Leu Leu Pro Gly Pro Leu Glu Asn Thr Gln Ile Pro

450 455 460

Arg Thr Ser Pro Leu Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn

465 470 475 480

Cys Val Val His Val Leu Met Phe Trp Leu Pro Met Arg Ser Leu Gly

485 490 495

Glu Pro Trp Arg Ala Gly Phe Gln Trp Gly Glu Ser Pro Trp Gln Val

500 505 510

Met Ala Ser Leu Thr Gly Pro Tyr Pro Ile Ser Glu Gly Leu Thr Met

515 520 525

Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu Met Thr Asn Cys Leu

530 535 540

Phe Ser Pro Ile Phe Leu Cys His His Ala Ile Met His Arg Lys Met

545 550 555 560

Thr Ser Pro His Phe Arg Leu Phe Ser Ser Lys Ile Pro His Pro Gln

565 570 575

Val Pro Ser Val Val Ala Leu Cys Lys Phe Arg Ala Thr Phe Leu Leu

580 585 590

Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala Arg Leu Cys Leu Ile Val

595 600 605

Ser Arg Thr Leu Leu Leu Val Gln Ser Ala Ala Pro Pro His Arg Leu

610 615 620

Leu Gly Pro Ser Arg Trp Ser Arg Ser His Leu Asp Phe Ser Pro Tyr

625 630 635 640

Trp Met Lys Lys Val Lys Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile

645 650 655

Phe Val Leu Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly

660 665 670

Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile His His

675 680 685

Arg Lys Asn Pro Leu Leu Pro Met Arg Ile Pro Ser Leu Asn Ala Gln

690 695 700

Trp Met Phe Leu Arg Ile Leu Leu Leu Ala His Met Tyr Gly Phe Ser

705 710 715 720

Thr Tyr Gly Thr Gln Lys Gly Glu Cys Val Cys Leu Gln Cys Leu Ser

725 730 735

413

Gly Ser Ser Leu Phe Ile Leu Lys Gln Pro Leu Asp Leu Lys Ile Ser

740 745 750

Lys Thr Phe Met Leu Gln Ala His Pro Gln Val Pro Ala Leu Ala Pro

755 760 765

Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala Pro Ser

770 775 780

Ala Gln Gln Ser Leu Thr Ala Ser Pro Gln Ile Met Gln Arg Met Ser

785 790 795 800

Ala Val Asn Phe Glu Thr Ile Ile Tyr Pro Ser Thr Cys Ser Leu

805 810 815

Pro Cys Arg Gly Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu

820 825 830

His Thr Gln Cys Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly Trp

835 840 845

Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys Val

850 855 860

Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly Ile

865 870 875 880

Cys Trp Lys Trp Pro Val Thr Val Ala Ala Gln Trp Ser Ser Val Thr

885 890 895

Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys Ser Ser Ser Trp Leu Met

900 905 910

Lys Gln Thr Trp Met Lys Ser Trp Ser Leu Arg Asp Pro Ser Tyr Arg

915 920 925

Ser Ile Leu Glu Tyr Val Ser Thr Arg Val Leu Trp Met Pro Thr Ser

930 935 940

Thr Val Arg Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln Leu Ala

945 950 955 960

Ile Gln Val Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe Gly Arg

965 970 975

Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu Ile Ala His

980 985 990

Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro Arg Leu Leu

995 1000 1005

Thr Arg Lys Lys Pro Gly Lys Phe Leu Pro Ser Arg Phe Pro Trp

1010 1015 1020

Gly Pro Pro Pro Leu Cys His Pro Ala Trp Leu Ser Pro Ile Ser

414

1025 1030 1035

Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His Val Leu Ala Leu

1040 1045 1050

Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro Gln Met Gln Leu

1055 1060 1065

Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys Arg Lys Arg

1070 1075 1080

Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln Glu Lys

1085 1090 1095

Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala Cys

1100 1105 1110

Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe

1115 1120 1125

Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val

1130 1135 1140

Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met

1145 1150 1155

Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe

1160 1165 1170

Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln Asn

1175 1180 1185

Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile

1190 1195 1200

Leu Thr Arg Arg Lys Lys Arg Glu Asp His Cys Gly Leu Leu Ile

1205 1210 1215

Leu Ile Gln His Gly Gly Ile Phe Thr Phe Arg Phe Trp Val Glu

1220 1225 1230

Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp

1235 1240 1245

Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr Ile Ile Glu Thr Asn

1250 1255 1260

Val Phe Val Leu Asn Leu Phe His Val Pro Val Asn Gln Ile Phe

1265 1270 1275

Met Met Ala Phe Tyr Leu Lys Tyr Thr Glu Lys Ala Trp Val Met

1280 1285 1290

Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys Tyr Gln Gln Glu Lys

1295 1300 1305

415

Lys Ser Arg Lys Leu Met Ser Ser Cys Arg Ala Phe Ile His Thr

1310 1315 1320

Pro Arg Ser Pro Gly Cys Ser Val Tyr Ala Asn Met Cys Trp Ala

1325 1330 1335

Phe Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr

1340 1345 1350

Gly Cys Trp Gly Ser Pro Pro Leu His Leu Cys Gln Pro Leu Arg

1355 1360 1365

Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg Arg Arg Lys Trp

1370 1375 1380

Gln Ile Arg His Val Leu Lys Asn Leu Ile Leu Phe His Val Arg

1385 1390 1395

Asn Ile Val Tyr Gly Ile Gln Val Met Leu Cys Gly Lys Gly Leu

1400 1405 1410

Thr His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn Lys Arg Asn

1415 1420 1425

Lys Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile Lys Arg

1430 1435 1440

Lys Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro Glu Ser

1445 1450 1455

Asn Pro Lys Phe Phe Leu Thr Phe Ser Gln Leu Glu Lys Lys Ala

1460 1465 1470

Thr Trp Met Arg Arg Thr Gly Ala Leu Arg Ala Thr Ala Pro Ala

1475 1480 1485

Thr Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg Ala Ala Thr

1490 1495 1500

Thr Ser Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr Ser Ser

1505 1510 1515

Ser Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser Ser Cys

1520 1525 1530

Tyr Trp Ser Leu Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu Ala

1535 1540 1545

Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly Arg Met

1550 1555 1560

Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys Phe

1565 1570 1575

416

Thr Gly Val Arg Thr Ser Ser Gly Ser Cys Gly Arg Ala Cys Ser

1580 1585 1590

Cys Gly Arg Gly Cys Gly Thr Pro Arg Gly Asp Trp Lys Arg His

1595 1600 1605

Pro Pro Arg Arg Asn Ser Val Ile Val His Lys Ile Val Leu Val

1610 1615 1620

Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu Arg Arg Ile Ser

1625 1630 1635

Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu Lys Leu Leu

1640 1645 1650

Asn Met Asp Leu His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu

1655 1660 1665

Leu Val Thr Cys Ile Leu Tyr His Arg Trp Ile Val Thr Ser Met

1670 1675 1680

Cys Gln Ser Gln Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser Ser

1685 1690 1695

Ala Leu Met Trp Thr Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg

1700 1705 1710

Lys Lys Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met

1715 1720 1725

Arg Pro Pro Arg Ser Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala

1730 1735 1740

Ser

<210> 1121

<211> 1749

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 3B poly-FSP

<400> 1121

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

417

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Gly Ala Ala Ala Ala Ser Cys Gln

225 230 235 240

Ser Pro Pro Ser Pro Arg Glu Thr Thr Pro Ser Ser Asp Ala Thr Arg

245 250 255

Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys Thr Met Thr Ala Ala

260 265 270

Gly Leu Thr Pro Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys Met

275 280 285

Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala Asn Arg Arg Asn Ser

290 295 300

Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu

305 310 315 320

Arg Val Arg Gly Pro Pro Leu His Thr Arg Asn Ile Arg Lys Leu Ile

325 330 335

Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe Pro Lys Leu Arg Ala

340 345 350

418

Thr Glu Gln Met Thr Asn Val Gly Cys Ser Ile Pro His Val Pro Lys

355 360 365

Ala Thr Ala Ala His Ser Val Thr Val Lys Leu Gln Leu Pro Leu Thr

370 375 380

Phe Ser Thr Leu Leu Trp Asp Ser Arg His Thr Met Ser Leu Ser Asn

385 390 395 400

Ile Leu Met Lys Phe Arg Leu Gln Ser Ser Pro Phe Leu Ala Arg Ser

405 410 415

Trp Asn Thr Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile

420 425 430

Ala Gly Ala His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp

435 440 445

Lys Met Asn Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe

450 455 460

Ser Pro Leu Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly

465 470 475 480

Lys Arg Cys Asn Pro Cys Cys Arg Leu Thr Trp Met Ser Gln Asn Thr

485 490 495

Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln

500 505 510

Arg Arg Gly Lys Leu Ser Ala Phe Ser Gly Arg Ile Leu His Gln Pro

515 520 525

Glu Met Ala Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr Asn

530 535 540

Ser Trp Trp Thr Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu Ala

545 550 555 560

Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro Thr Thr Arg Leu Met

565 570 575

Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu Leu Thr Gln Pro Ser

580 585 590

Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu His Pro Gln Trp Ala

595 600 605

Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys Thr

610 615 620

Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr Trp Gly Pro Val Tyr

625 630 635 640

419

Tyr Val Cys Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Phe Leu

645 650 655

Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys Thr Leu Gly Arg Asn

660 665 670

Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys Leu Ile Pro Val Pro

675 680 685

Ile Ser Glu Lys Lys Arg Lys Leu Pro Asn Ile Glu Ile Thr Pro Gln

690 695 700

Arg Ser Lys Ala Met Leu Gln Lys Gln Cys Cys Tyr Asn Leu Val Ile

705 710 715 720

Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg Arg Gln Gly Lys Thr

725 730 735

Lys Asp Gly Val Val Gln Ser Leu Gly Phe Leu Asn Gln Gln Ile Ile

740 745 750

Leu Gln Lys Glu Phe Phe Ile Cys Leu Leu Met Phe Ile Lys Arg Trp

755 760 765

Lys Pro Ala Ala Thr Lys Ser Arg Lys Lys Gly His Thr Lys Asn Arg

770 775 780

Arg Lys Ala Lys Arg Lys Pro Gln Ile Tyr Leu Phe Phe Leu Thr Val

785 790 795 800

Cys Gly Phe His Leu Lys Lys Thr Ser Ala Lys Trp Leu Lys Lys Ser

805 810 815

Thr Lys Ser Arg Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr

820 825 830

Pro Arg Leu Ser Cys Leu Arg Val Arg Cys Gly Gly Trp Leu Cys Gly

835 840 845

Trp Thr Pro Arg Gly Arg Lys Ser Gly Val Met Lys Cys Trp Asp Leu

850 855 860

Cys Pro Val His Lys Lys Arg Gly Leu Phe Arg Leu Asp Phe His Pro

865 870 875 880

Arg Ser Ser Ser Gly Lys Gly Lys Asn Ser Pro Cys Cys Gln Lys Lys

885 890 895

Leu Arg Val Gln Asn Gln Gly His Leu Leu Met Ile Leu Leu His Asn

900 905 910

Asp Arg Val Phe Leu Val Lys Ile Gly Glu Lys Leu Lys His Cys Phe

915 920 925

Lys Ala Trp Gly Ser Arg Arg Asn Arg Arg Tyr Arg Lys Ile Tyr Gly

420

930 935 940

Glu Leu Lys Met Ser Leu Gln Ala Phe Leu Ile His Leu Arg Lys Val

945 950 955 960

Asp Thr His His Leu Gln Val Val Thr Lys Thr Val Thr Ala Thr Thr

965 970 975

Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys Lys Ala Cys Arg Leu

980 985 990

Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser His

995 1000 1005

Phe Pro Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln

1010 1015 1020

Leu Arg Ser Leu Phe Phe Ser His Cys Leu Cys Ser Gln Lys Lys

1025 1030 1035

Asp Pro Ala Gly Lys Leu Lys Lys Leu Leu Lys Lys Ser Ser Gln

1040 1045 1050

Leu Arg Arg Ser Trp Arg Asn Val Ser Arg Ile Ser Thr Pro Pro

1055 1060 1065

Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro Val Ser Val Ile Thr

1070 1075 1080

Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn Leu

1085 1090 1095

Val Val Trp Ile Leu Asn Leu Lys Met Met Thr Tyr Met Glu Met

1100 1105 1110

His Tyr Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu

1115 1120 1125

Leu Cys Arg Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg

1130 1135 1140

His Pro Gln Gly Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val

1145 1150 1155

Gln Ser Val Leu Leu Leu Gly Val Pro Gln Thr Ala Arg Gly Pro

1160 1165 1170

Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln Ser

1175 1180 1185

Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg Thr Glu Val

1190 1195 1200

Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser Pro Gly Pro

1205 1210 1215

421

Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala Ile Gly Lys

1220 1225 1230

Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala

1235 1240 1245

Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr Met Val Met Lys

1250 1255 1260

Leu Cys Phe Phe Tyr Leu Leu Tyr Arg Leu Arg Ile Pro Ser Leu

1265 1270 1275

Arg Lys Tyr Arg Gln His Leu Asn Arg Lys Leu His Leu Thr Arg

1280 1285 1290

Ser Cys Leu Ile Asp Leu Thr Met Met Met Ser Gln Lys Leu Trp

1295 1300 1305

Lys Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro Arg Gln His

1310 1315 1320

Leu Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro Cys Leu Leu

1325 1330 1335

Leu Leu His His Arg His His Leu Ala Phe Leu Glu Met Ala Cys

1340 1345 1350

Ser Ser Gln His Thr His Ser Ile Lys Ile Trp Ile Ser Phe Ser

1355 1360 1365

His Glu Val Asp Pro Pro Gly Ser Val Trp Ile Leu Pro Cys Asp

1370 1375 1380

Leu Pro Arg Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln

1385 1390 1395

Met Pro Phe Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr

1400 1405 1410

Trp Glu Thr Pro Ser Thr Trp Gly Trp Arg Thr Phe Arg Gly Ala

1415 1420 1425

Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe Phe Thr Ser Ala

1430 1435 1440

His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln Cys Lys Lys

1445 1450 1455

Lys Leu Leu Leu Lys Leu Leu Thr Thr Thr Thr Ser Ser Pro Arg

1460 1465 1470

Arg Ser Pro Ala Leu Arg Ala Arg Gly Gly Thr Thr Ile Gly Glu

1475 1480 1485

422

Val Thr Ser Cys Thr Asn Leu Ser Val Pro Met Met Leu Thr Ile

1490 1495 1500

Leu Ile Trp Lys Arg Val Phe Ile Leu Leu Leu Ser Asp Lys Lys

1505 1510 1515

Gly Ala Lys Lys Ile Thr Phe Leu Pro Ile Pro Pro His Leu Arg

1520 1525 1530

His Pro Leu Leu Leu Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser

1535 1540 1545

Met Asp Leu Asp Gly Met Leu Thr Val Ile Gly Gln Gly Leu Val

1550 1555 1560

Gly Met Thr Pro Arg Asp Leu Gly Thr Val Ala Gly Ala Thr Ala

1565 1570 1575

Gln Ile Gln Arg Pro Ile Gln Val Gln Thr Gly Gly Arg Leu Arg

1580 1585 1590

Arg Asn Leu Gln Arg Asn Leu Lys Val Leu Gln Val Ser Val Tyr

1595 1600 1605

Ser Gly Asn Leu Leu Ser Phe Ser Arg Arg Gly Met Lys Ser Ser

1610 1615 1620

Val Arg Leu Lys Asn Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu

1625 1630 1635

Gly Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu Lys Arg Ile Leu

1640 1645 1650

Ile Arg Lys Ala Met Thr Val Leu Ala Gly Asp Lys Lys Gly Leu

1655 1660 1665

Gly Arg Phe Met Arg Cys Val Gln Ser Glu Thr Lys Ala Val Ser

1670 1675 1680

Leu Gln Leu Pro Leu Gly Arg Asn Met Glu His Arg Leu Thr Met

1685 1690 1695

Asp Ser Ala Leu Ser Leu Thr Lys Arg Glu Gly Pro Glu Thr Leu

1700 1705 1710

Lys Lys Lys Tyr Leu Val Gln His Gln Met Lys Cys Leu Thr Ala

1715 1720 1725

Val Asp Cys Leu Gln Ala Phe Trp Asp Met Gly Tyr Pro Tyr Asp

1730 1735 1740

Val Pro Asp Tyr Ala Ser

1745

423

<210> 1122

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 4B poly-FSP

<400> 1122

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Ile Gln Lys Lys Lys Gln Lys Ser

225 230 235 240

Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys Lys Lys Lys Asp Lys

245 250 255

424

Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn Ser Thr Glu Cys Gln

260 265 270

Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn Leu Val Gln Leu Asn

275 280 285

Pro His Ser Pro His His Arg Lys Asp Glu Glu Asp His Gln Lys Arg

290 295 300

His His His His Asn Gln Lys Lys Met Ser Val Met Ala Lys Lys Ser

305 310 315 320

Gln Gln Pro Leu Gly Gln Ala Phe Gly Ser Leu Cys Gln Leu Val Ser

325 330 335

Ala Asp Phe Ser Ala Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His

340 345 350

Leu Leu Ile Asn Glu Gly Asn Asn His Trp Pro Arg Val Glu Met Pro

355 360 365

Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn

370 375 380

Pro Arg Leu Gln Gln His Leu Lys His Trp Gln Gln Cys Thr Arg Ala

385 390 395 400

Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe

405 410 415

Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly

420 425 430

Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val

435 440 445

Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp Leu Ile Cys Phe Val

450 455 460

Phe Phe Val Cys Val Glu Thr Gly Phe Cys His Val Ala Gln Val Gly

465 470 475 480

Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp

485 490 495

Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro

500 505 510

Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala Arg Leu Arg Ala Ser

515 520 525

Val Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly Val Arg Thr Arg Gly

530 535 540

425

Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg Asn Ile Leu Asn Ser

545 550 555 560

Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu Asn Arg Lys Thr

565 570 575

Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp Asn Leu His Leu

580 585 590

Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg Gln Met Glu

595 600 605

Ser Leu Gly Met Lys Leu Gln Ser Pro Leu Trp Asp Cys Ser Thr Thr

610 615 620

Thr Gly Thr His His Gly Cys Leu Trp Glu Ile Pro Ser Val Thr Leu

625 630 635 640

Leu Ala Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg Arg Met

645 650 655

Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr Val Arg Leu

660 665 670

Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp Val Val Ile Asn

675 680 685

Leu Lys Arg Leu Gln Lys Phe Ser Gln Lys Ser Gln His His Cys Ala

690 695 700

Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly Lys Ala

705 710 715 720

Gly Pro Gln Ala Gln Glu Lys Ala Asn Ala Lys Lys Met Pro Phe Gln

725 730 735

Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met Leu Cys Leu Pro

740 745 750

Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile Ser

755 760 765

Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu Ala Pro Leu Phe Phe

770 775 780

His Gly Asp Leu Cys Glu Glu Lys Lys Asn Gly Arg Ile Ser Ser Gln

785 790 795 800

Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe

805 810 815

Gly Ser Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys Cys Glu Trp Gly

820 825 830

Gly Gln Ala His Arg Gln Arg Asn Thr Gly Met Glu Ile Leu Leu Trp

426

835 840 845

Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu

850 855 860

Met Gln Leu Cys Thr Tyr Thr Pro His Ile Cys Arg His Thr Cys Arg

865 870 875 880

Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser Cys Ser Pro Ser Gly

885 890 895

Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln Gly Cys Leu Arg Pro

900 905 910

His Arg Val Gln Leu Leu Arg Arg Trp Gly Pro Gly Ser Pro Ala Gly

915 920 925

Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg Trp Trp Gly Pro Gly

930 935 940

Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe Pro Pro Pro Asp Pro

945 950 955 960

Pro Trp Pro Val Thr Ala Val Thr Val Met Ala Gly Ser Val Pro Ser

965 970 975

Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly Pro Leu Ala Leu Glu

980 985 990

Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg Glu Met Ser Ile Phe

995 1000 1005

Leu Pro Gly Ile Phe Thr Ser Arg Ile Leu Asn Leu Gln Val Leu

1010 1015 1020

Lys Lys Ile Leu Arg Ser Phe Met Lys Leu Tyr His Ser Leu Val

1025 1030 1035

Met Cys Leu Arg Leu Arg Thr Lys Leu Glu Lys Ala Leu Ser Ala

1040 1045 1050

Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Gln Arg Pro Pro Leu

1055 1060 1065

Gln Pro Val Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe

1070 1075 1080

Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys

1085 1090 1095

Met Leu Asn Gln Lys Cys Leu Ala Pro Phe Ile Thr His Pro Phe

1100 1105 1110

Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile

1115 1120 1125

427

Arg Leu Leu Lys Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe

1130 1135 1140

Ser Asn Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu

1145 1150 1155

Lys Gln Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile

1160 1165 1170

Pro Asn Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp

1175 1180 1185

Trp His Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val

1190 1195 1200

Ser Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly

1205 1210 1215

Met Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser

1220 1225 1230

Trp Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Leu

1235 1240 1245

Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile Ser Asp Ser Lys Lys

1250 1255 1260

Met Thr Gln Lys Arg Glu Ser Thr Leu Gly Asn Phe Trp Met Lys

1265 1270 1275

Leu Leu Gln Leu Pro Asp Lys Lys Lys Lys Ser Thr Glu Lys Thr

1280 1285 1290

Lys Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val His Met Cys

1295 1300 1305

Ser Ser Leu Ala Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu Pro

1310 1315 1320

Ser Ser Asp Leu Ala Val Leu Ile Ser Ser Ser Thr Ser His Phe

1325 1330 1335

Leu Met Arg Ser Pro Val Leu Pro Ser Ser Arg Leu Thr Cys Ala

1340 1345 1350

Ser Pro Gln Leu Pro Arg Met Trp Thr Trp Ser Ser Ser Trp Leu Lys

1355 1360 1365

Trp Leu Lys Asn Trp Lys Arg Phe Lys Leu Lys Lys Lys Tyr Ile

1370 1375 1380

Leu Ala Met Thr Leu Thr Leu Asn Gly His Pro Arg Thr Ala Ser

1385 1390 1395

428

Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly Ser Arg Thr Ser Cys

1400 1405 1410

Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr Lys Trp Pro Leu

1415 1420 1425

Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr Leu Val Trp

1430 1435 1440

Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala His Cys Pro

1445 1450 1455

Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser Ser

1460 1465 1470

Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala

1475 1480 1485

Val Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile

1490 1495 1500

Gln Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser

1505 1510 1515

Trp Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys

1520 1525 1530

Arg Trp Pro Arg Arg Glu Leu Lys Lys Asn Leu Leu Leu Pro Cys

1535 1540 1545

Arg Cys Arg Arg Glu Tyr Arg Val Thr Met Gly Gln Ala Phe Gly

1550 1555 1560

Ser Phe Ser Ala Asp Phe Ser Ala Pro Gln Val Leu Lys Lys Asn

1565 1570 1575

Arg Lys Gln Asn Lys Ile Ile Cys Lys Gly His Arg Ile Thr Ser

1580 1585 1590

Leu Ile Leu Ser Phe Gln Arg Val Lys Leu Lys Lys Lys Val Asn

1595 1600 1605

Ala Cys Ser Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys

1610 1615 1620

Leu Val Gly Arg Ala Val Arg Arg Lys Gly Ile Ile Cys Ile Lys

1625 1630 1635

Asn Met Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu Ser His Val

1640 1645 1650

Glu Lys Phe Val Pro Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg

1655 1660 1665

429

Met Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro

1670 1675 1680

Pro Val Trp His Ile Leu Gly Pro Gln Arg His Thr Pro Cys Gln

1685 1690 1695

Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu Arg Pro Ser Pro

1700 1705 1710

Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro Gly His Ala Leu

1715 1720 1725

Ser Cys Arg Thr Arg Arg Arg Trp Ser Ser Lys Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1123

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 1A poly-FSP

<400> 1123

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaacc gagatccagc tgaccatgaa cgacagcaag 120

cacaagctgg aaagccccgc tctgaaacag gtgtcaccag cctctccacc tactcagcag 180

ccctcagacac ctcaggattc tagacaggtg ctggccctgg ccaagaaaac cctgacaaat 240

gcctccagag ccggacagtt tcctgtgtgg cggagaacaa gcagaggcat gagcctgcag 300

tggaagtccg gcaatgcctg cacacctctg agatgcccta gcagaagccc tgccacattc 360

ctgacagccc tgaatggctg gcacagatct ggcctgagag cccagatgcc tagctcttgg 420

tctagcagcg gcagaacact gaagctggcc cctctgcaag tgaccaccac tctgaagctg 480

atcctggtca tgctggaaat cgtgaccgag cgctccctgc tgtactccag aatgtgtctg 540

gtgcagatcc ccacacacac ctgggagccc gattacttcg tgtccctgcc tatgatgttc 600

agctgggcca cacagtcttt tcccgtgggc gccgtgtttc tgaccagacc tgccattcct 660

tgctgcagct gtaccagcca cagcaaccct tggagccaga gcaatcagca gctgaagtgg 720

acccctcaca tcctgaagtc cgccagcttc gacaagaaag gcttcgtgat cctgagccgg 780

cggagcaaga ttgaggcctc tcctatcggc tctatcgcca gctctcctgg acatcctctg 840

ggacctacac tggccatcag attcatcagc gcccctcagc ctagcagctc tacagtgaca 900

gctgccccta gcggccagta ctttatcgcc aagaagcgga tcaagctgcg gcggaacaat 960

atgggcggca acagatccat ctgcctgacc agaaagcgca agagagagaa gcggagccag 1020

aagtccaggc agaaccatct gcagctgaaa agctgcagac ggaagatcag caactggtcc 1080

aagctgagaa gcccctggat cttcaccaag tgcgagatct gcgtgaagaa caccgccatc 1140

ctggctatca ccctgatcac ccctcaggac cctcatctgg accctgtgtg tcaccctcat 1200

agaaggcctg gatggaaggg ccaccataga gtggtgttct ctcaccatcc tagcggcagc 1260

acaaagagcc actggcagca aagagaacgc ggaggcggca gacgcgagaa gaagggatgt 1320

agagaacagc ggaaggtggt caagaacccc tctcagcccg tgcctctgag aaacgatggc 1380

atcaccaacg gcaacgactc caccagaagc tgctccatca gcctgaagaa aagcctggaa 1440

aagatgcccc tgatgcggag agccagacct accagctgta gcacctgtag aatgtggtgg 1500

aagggcaaga aacggcggaa agaaatcgtg cctatgagcc tggcctgcag catcaccgtg 1560

tcttttgtgg gacagatcag caccctggtg ctggatgtgt ctagcatcag ccattggcgg 1620

ctgccctgta aagtgaccga gagcagctcc agcttcttct acaccagcga caggacccct 1680

ctgagcatca gcagaccaac cagcagagct agacagagct gttgtctgcc cggactgaca 1740

caccctgctc accaacctct gggcagcatg agcactagaa gagagatgcc aagccaggcc 1800

acaccttctc cacctggacc ttggagagcc gctctgggca aagaagtgaa gagatcccag 1860

agcaagctgc gcctgagaaa cacaagccct ggggagaaga acgatctcca gctgttcgtg 1920

atgagcgaca ccacctacaa gatctactgg acagtgatcc tgctgaaccc ctgcggcaac 1980

ctgcacctga aaacaacatc actgcagcgg ccacctcgga gcatcctgtc tctgaatgga 2040

atctgggaag tgatgccccg gacactggaa ggctgcatca gcgtgaagga tcacagaacc 2100

agccagaacc acagcaagtg catcaccgaa tgtgtgcgga ctgccctgaa cgtgtccaag 2160

aagagactgc ctaagctgat gaagaagaag gacagcacca tcttcatcaa caaagtgcgc 2220

cagaacttca agcgcaactt caagaagaag aaacccctca ctttcagccc cagcagacgg 2280

atgactcgga gaaccatgac aaccaccaaa atgatgatga tgcggcggac caagcggaga 2340

aggcgcaaaa ggcggagaat gaccatgatg accagacgga ccctgctgac aaagaaaacc 2400

tgtctgaccc ctagaaccag cgccgccaca aaagctctgc ctagacagac catcagccag 2460

cggaggccta ttcagacccc acctggaaca tctctggcca tctggggcag aacaagactg 2520

accctgacag gcaccagatc cagcggaaca agagcctctc cttccgccgt gtgcctgtgt 2580

tgttggacct gtccaagcag aagcttccca gccgcttctc ctagtggact gagagcacct 2640

gttggagccc aggctcctcc tgaagtgaca ctgagagcct cttgtgcctc tagcgcctct 2700

aaagcctgtg ctggactttg gccttgggga gcccaagttc gagttggagc tggaacaggc 2760

ggaggacctg cttgtgttcc tgtggctgaa gcttctacag gcgcatggac agctgaaggc 2820

gccgctggaa caagatgtag agcctctaga gccccttgcc gccaacttcc tgctagctcc 2880

cctaccaagt gtgaaatttg tgtgaagcgc agacggcgga gaaagagaag cagccagtct 2940

agaagccccg tgcctaaaaa gcgcgagagg atgatgctgc ctgtgcgggc cagaacagct 3000

agatctgcac ttcctcaggg cagagcacct ctggaaggga tgctgcttcc tagagcactg 3060

cctagcctgc tgagcagaat gcctgccaga agagagtccc ggttttggct ggctctgcag 3120

gcacttggac tcagacctcc acatctgcct agctgtcatc ctctgcctca actgttgggc 3180

cctccacctc ctcatcaggc cccaatgaca ctgaaggccc tggttaagac ctggacacag 3240

ttccgcaact accccagagc cgacaagaag aatgtccggt ctaccgcctt cttcctgctg 3300

gacatcatcc tgggcgattg cccaagaaca atggccggac cttctgcctc tctgcatcct 3360

gcatctggca gagcctccag aaagacctgg cagagaaggc tgagagcttg tagagcaggc 3420

ggacctgctg gatggggctag acaggcctat cctagactga aaaccttcgg cgtgcccctg 3480

ggctctattc tgtgtctggc cggcagcctg tccacaatga tgatcattag caccaccaga 3540

caagagtgcg gcaggcgggc ttctgtgcct tgtagaccta tgatcggaag cgccagacca 3600

ggaccttggc ggacatctgc tatgcctagt gccatgggag tcgccctgcc tacctcttgt 3660

gaaagcggaa gatctcctcc tgccaccggc ggaagaatgc caggatctca gtctatcatg 3720

atgagctgga tgcctccact ggctccatgt gccgcttgtc catgttctcc cgctcctaca 3780

ctgtgcccag cacatcctgc tagagcacca acagccgtgc ctgcctttac acctctgtct 3840

gctcatcctg tgccagtgct gtctggatgt cacctggccg tcagaacctc catgctgaca 3900

3960

atgatctggg agagccggct gccactgagg ccacaaagag gacctagacc tctgcacaga 4020

tggctgcagc agacaaccgg ctgtagccag aggcggatcg tgttctttct ggccccatac 4080

ttccctcggc accctcacag atctccacca acatcttgcc cacctctgct ggaagctttc 4140

ccactgccaa gagcttctct gagggccaca gtgccaagac atagctctgt tctcgccacc 4200

ggtctgcacc tccacagaca gatttctctg cccctgagag gattcctcct gcaccaacca 4260

ctgccaggac agcatctgtg gctgtctcac agactgaggc tgagactgcc caggacacag 4320

ccatctttgc ctggaccatg tgcaagcctg ctgtccacac tgtctcagcc accacctcag 4380

gctccatctc aagtgtggac tgccgctaca ctgagatgtc ctgctgttcc tgcagctgcc 4440

tgtcctcctg ctctgagatc catgcctaag agccggaacc aggtgctgtt cagacggaat 4500

ctgaccccaa gccagctgag aacactggcc ccaccttata tgctgctgcc ccagtctctg 4560

tcccagagcc tgagtagaaa gcagatccct agccagtcca tgctcgtggg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1124

<211> 4623

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 2A poly-FSP

<400> 1124

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaaga aagatgcaga gcctgaatca ccccagcaga 120

ctgaccaatg gctctcctcc actgcacctg tgccagcctc tgagaatgag cagaacccct 180

cctagaagag tgtgcgccgc tcctccacat agactgctgg gcccttccag atggaaccct 240

cggagaaaga cctggaagat gcggaagaag tacgcccaga agcctagcca gaagaaccac 300

ctgtacggcg gcaagaagcg ctctaagttc agcagaagcc acctggactt cagcccctac 360

tggatgaaga aagtgaaggt gctgttcaag gccgaccaga gcgagtctag cctgagcagc 420

ggaagatgca gcctgatgga acagatccct cacctgaagt ctcggcggaa gtggcagatc 480

agacacgtgc tgaagaacct gtggctgccc atgagaagcc tgggagaacc ttggagagcc 540

ggctttcagt ggggagagtc tccttggcaa gtgatggcct ctctgaccgg cagagccaca 600

tttctgctgg ccctttggga atgtagcctg cctcaggcaa gactgtgcct gatcgtgtct 660

agaaccctgc tgctggtgca gagccggaac aagaaaacaa agctgtggtt cagcctgatc 720

aacatccacc accggaagaa cccactgctg cctatgagat ggtggtgtac cagaggcggc 780

ggaaagcgga aaaagaaaat gtgcaaggtg tccggcggct gcaccgcctg tgttgagaga 840

tgtccttgga agggcatctg ctggaagtgg cctgtgacag tggccgccat tagaatgtgt 900

gccctgtctc accctagctt ctacatcagc agcaccgtgt gctttctgag caagcactgc 960

atgaccgcct gcaccagaaa gagaatgaag accggcgcca tccaagagaa gggcgctatt 1020

caagagaaag gggccatgac caagcggatc tgcgtggtca atttcgaggc ctgcagagag 1080

atggaaagcc tgagcgcccc tgagaagatc accctgtttg aagcccacat ggcccggtgc 1140

acctccatca atgtgctgtg tgtgcgggcc agcctgattg agaagctgat gaaagaaaag 1200

cgcaagatga agcggaacca ggtggcctct ccacagatca tgcagcggct gttcatcaag 1260

ggctaccaga acaaccactt caccttccat ctgcaaaact tcgccttctt cagctccttc 1320

ggcaacaaca agtacatcaa tatcctgacc atcctgttcc acgtgcggaa catcgtgtac 1380

ggcatccaag tgatgctgtg cgctagccct cagattatgc agagaaccag cgccgaccgg 1440

ttccggatga agagaagaat gcagaatctg gacaacctgg gcgtgccatg gcctctgttg 1500

cctggaatcc catctctgaa cgcccagtgg atgttcctga gaatcgcatc cccgcaaatc 1560

atgcagagga tgagcgccgt gaacttcgag acaatccggg ccagaaaccg gaacatgaag 1620

aaaaccatga gccacctgat cgcccatcag gccctgaaga tgagcggcct gagactgttt 1680

caccccagac tgctgacacg gaagaagccc ggaaaactgc tgctcgccca tatgtacggc 1740

ttcagcacct acggcaccca gaaaggcgag tgcgtttgtc tgcagtgtct gagcggcagc 1800

agcctgttca ttctgaagca gcccctggac ctgaagatca gcaagacctt catgggccac 1860

agagagggca gcgagtctga taatctgggc ctgctgtata gcttccctcc accttatcct 1920

atcagcgagg gcctgaccat gaccaccggc agaaacatca acagcaactc cctgatgacc 1980

aactgcctgt tcagccccat cttcctgtgc caccacgcca tcatgcacag aaagatgaca 2040

agccctcact tcaggctgtt cagcagcaag atcccacatc ctcaggtgcc atctgtggtg 2100

gccctgtgca agttccgggc cttcatccac acacctagaa gccctggctg cagcgtgtac 2160

gccaatatgt gctggcccaa gttcttcctg accttcagcc agcagtggtc cagcgtgaca 2220

tctctggaat ggacagccca agagagaggc tgcagcagct ggctgatgaa gcagacatgg 2280

atgaagtctt ggagcctgcg ggaccccagc tacagatcca tcctggaata cgtgtccacc 2340

agagtgctgt ggatgcctac ctctaccgtg ctgcaggctc atccacaagt tccagctctg 2400

gctcctcaag cctggtggcc tgctagaaga ggacctctga catctgcccc atccgctcag 2460

cagtccctga ccagagagaa gattatcaac cccaccatca gctgcccctt tcagagcccc 2520

accaagagac tgatctaccc tcctagcacc tgttctctgc cctgcagagg cttcctgcct 2580

agcagatttc cttggggccc acctcctctg aacagcgtga tcgtgcacaa gatcgtgctg 2640

gtgttcctga agtccctgtc cagctacaac tgcgagcgga gaatcagcgg catccagaag 2700

agagccatcg agttcgccgt gctgaaactg ctgaatatgg acctgcctct cgagaacaca 2760

cagatcccca gaacaagccc actgtggaag atcacaatgg tgctgacata ccccaactgc 2820

gtggtgcatg tgctgatgtt tggcgccctg agagccacag ctcctgctac agctgcacct 2880

caggctcaga cccctcattg gagagctgcc acaacaagca gcatgagcag cagccctaga 2940

gccgagccta catcttctag ctcttgggcc acctgttggc ccagatgctg ctccagctct 3000

tgttactggt cactgtctag ctggcctcca gctggcgccg ctgaagctac aaataccaga 3060

accagaagcc gcgagagcca gaggggtaga atgctgactt ggagaagctc cctgtggttg 3120

cagggcacca gatgttttac aggcgtgcgg accagctcca gatacaagaa aagaggcgtg 3180

atcgcctggc ggagacagat caatagacgg aagctgcaga gaaagaagca ctggaacagc 3240

gcctacctgg gcagaacact gctggtcacc tgtatcctgt atcaccggtg gatcgtgacc 3300

tccatgtgtc agtccgag atggctgacc agcaccacaa gtagatctag cgccctgatg 3360

tggaccgagc tgagcctgga accatggatt ccctacctgc atcagcagaa aacccagcgg 3420

acactggaat ccgccggact gtttccttgg ctgcacaccc agtgccaaga gaagaaaagc 3480

agaaagctga tgtccagctg cacacacccc tgcacaatgc tgctgccaca gggatgtcat 3540

cctgcctggc tgagccctat ctctgcctgt ccaagactgc tgccatatcc ttttcacgtg 3600

ctggctctgg aagtgacctt tcctcctgga accgccatgc ctcagatgca gttgcaggct 3660

ctggctatgt gggagaaagg ctgggataag cctggcacca gcctgcagtg ctacctgaac 3720

agaagaagaa acagccggaa gcagagccag gtgcaactgg ccattcaggt gctgctcgtg 3780

gacaagcaca agtactttct gttcggccaa aagaaaggcc acatgatctt tagcctggcc 3840

aacggcctgt ggtttaacct gattttcaac tccgggaaga aactgctcga ggacatgaag 3900

caaaatggct tcaccggcaa aggcctgaca caccccgtgc ctctgaagcg gactctgcag 3960

aacaaaagaa acaagaatat caaacgcgtc aaccagaaga agggcaacat caagcggaag 4020

tccatcctgc tgcacctgat tgtgctgaat agccccgaga gcaacctgat cctgatccag 4080

catggcggca tcttcacctt tcggttctgg gtcgagaagc tgtcctggaa catctgcaag 4140

aacctgagcc tgtacctgga caagttcgtg cagagactgc tgtacgtgta catcatcgag 4200

acaaacgtgt tcgtgctgaa cctgtttcat gtgcccgtga accagatctt catgatggcc 4260

ttctacctga agtacaccga gaaggcctgg gtcatggaac tggaatggct gatccaacag 4320

cagtgctatc agggcagctg cggcagagct tgttcttgtg gaagaggctg tggcacccct 4380

agaggcgatt ggaagagaca tcctcctcgg agagccttct ggaagcctcc tagccagggc 4440

atctctagct gtctgatgac cggctgttgg ctcgagaaga aagctacctg gatgcggcgg 4500

acccgccgga agaagaggga agatcattgc ggactgagac accccagctg gccttggaca 4560

agatgtctga ggatgaggcc tccaagaagc ggctacccct acgatgtccc tgactatgcg 4620

tcc 4623

<210> 1125

<211> 4638

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 3A poly-FSP

<400> 1125

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgagga gctgctgctg cctcttgtca gtctcctcct 120

tctcctcggg aaaccacacc tagctccgac gccaccagac ggacaagacc tattgccgct 180

cagagcaaga aaaccatgac agccgccgga ctgaccccta tgatccggaa gatccagaag 240

cggcggagaa ccgccaaccg gtgtctgaga gttagatgtg gcggctggct gtgtggctgg 300

acacctagag gacagaacac cagaagcacc aacctggaaa agaaccccag cctgaagaag 360

cagagccagc ggagaggaaa gctgagcagc ctgttcttca gccactgcct gtgccggaac 420

agcagaaggg ccacacctac atctaagggc agcctgcggc ggaagtacct gagagtgcct 480

attcctccac acctgagaca ccctctgctg ctgatggacc tggacggaat gctgaccgtg 540

atcggacagg gactcgtggg catgacccct agagatctgg gaacagtggc tggcgccacc 600

gctcagattc agaggcctat tcaggtgcag accggcggca gacttacacc tcctcctctg 660

attctgtggc ctcaggccgc tcctgttgat cctcctggct ctgtgtggat tctgccctgc 720

aagaacaaga agcggagaaa gctgcggccc aagcggctgg actttcaccc tagatcttct 780

agcggcgtgt ccgtgatcac catgaagctg tgggagaagc acttcaaggg cctgaactgg 840

aacctggtcg tctggattct gaacctgaag atgatgaaca tcgagatcac ccctcagcgg 900

agcaaggcca gaaagtccgg cgtgatgaag tgctgggatc tgtgccccag cgagaagaag 960

aggaagctgc ctctgcctct gaccttcagc accctgctgt gggactctag acatagaggc 1020

gccagctcca gcaccagcag agactccaga agctttttca ccagcgctca ccccagcaga 1080

ccctgcagaa tgagactgta ccagttcctg atccacctga gaaaggtgga cacccaccat 1140

ctgcaggtcg tgaccaagac cgtgacagcc acaacagctg acggccagag aatgcggaga 1200

ctgaagtgca aggcctgccg gctgagatgg gctagacctg aacctgctgc tcaggcccct 1260

ccaaagagcc actttccacc tcatcctagc ggccctagca gaaccagttc tctgcagctg 1320

cggagcatcc ctcacgtgcc aaaagccaca gccgctcaca gcgtgacagt gaagctgcag 1380

aagggaaaga acagcccctg ctgccaaaag aaactgcggg tgcagaatca gggccatctg 1440

ctgatgatcc tgctgcacaa tacctacatg gaaatgcact acggccatca ggccccacag 1500

gtggccatgc tgcctaagct gctgtgcaga cagatgctgc ccactctgag cacactgcag 1560

caaagacacc cacagggccc ttgtcctcca cctgtgacac cactgagagt ccagtctgtc 1620

ctgctgctgg gagtgcctca gacagctgct agacagccta gcagacccac cggcagaacc 1680

cctaccacaa gactgatggc ccctgtcggc agcgtgatga gctgttctct gctgacccag 1740

ccttctccag ccagcgcctg tacaatgcct ccactgctgc atcctcagtg ggccccacat 1800

tctgctggat tgtctcccgg cgcttgcaga cctgcctgta cctgtatcag catgaccacc 1860

atctccagag ccacctggca ggtttccgtg tacagcggca atctgctgag cttctccaga 1920

cggggcatga agagtagcgt gcggctgaaa aacagccggc ggaacagacg gtacagaaag 1980

atctacggcg agctgaagat gtccctgcag gccgtggctc ctagatgtcc tagatctgcc 2040

gtggacatca gccccggacc tacctctcct agcctggcct cttgtgcctc tgtggccatt 2100

ggaaaactgg ccctgcttgg cgcttctgcc ccttgtggtg ctgctggcgc tagagaggct 2160

ggaagattttg ccgtgctggg caccatggtc atgaagcacc tgaaccgcaa gctgcatctg 2220

acccggtcct gcctgatcga tctgaccatg atgatgagcc agaagctctg gaagaaccag 2280

cggaagagaa tccctctgcc atctcctaga cagcatctgc tccctcagta ccctctgcac 2340

cctcctccac cttgtctgct gctgctccac cacagacacc acctggcctt tctggaaatg 2400

gcctgcagca gccagcacac ccactccatc aagatctgga tcagctttag ccacgagcag 2460

agcagcccat tcctggccag atcctggaac accatgcagt ggaacaacaa gaaaatggcc 2520

atgacagtga tcgctggcgc ccaccactgt cagtacaagc tgacctacac caaagtgtgg 2580

aagatgaatt ggaagaaaat gatgatcggc tcccagcacc tcctgttcag ccctctggat 2640

agctctccac tgcaactgct gcttcaccct cacggcaaga gatgcaaccc ttgctgcaga 2700

ctgacatgga tgtccatgct gcagaaacag tgctgctaca acctcgtgat caagcaccca 2760

ctgatcttca gaatcaacct gcggagacag ggcaagacca aggatggcgt tgtgcagagc 2820

ctgggcttcc tgaaccagca gatcatcctg cagaaagagt tcttcatctg cctgctcatg 2880

tttatcaagc ggtggaagcc tgccgccacc aagagagagt gggagaaacg caagcagatc 2940

ctcggcggag gcggcaagta caaaagtac ttcctgaagc gcatcctgat tcggaaggcc 3000

atgactgtgc tggccggcga caagaaaggc ctgggcagat tcatgagatg cgtgcagagc 3060

gagacaaagg ccgttagcct gcagctccca ctcggaagag gccctgtgta ctacgtgtgc 3120

aaccggatga acgtgtccag cgtgctgaag cccttcctgt tcaccgtgcc tatcgaggac 3180

atgagagagt gcacactggg ccgcaacaga atgaatgtgt cctctgtgct gaaaccctgt 3240

ctgatccctg tgccaatcga cctgcctcgg atgctgccaa tcagcagccc actgcacaga 3300

3360

ccttctacat ggggctggcg gacctttcgg ggccctagaa gaaaagtgct gatggtgctg 3420

3480

gagaacatcc ggaagctgat ctggaccaag cagcggagct tcctgtctct gttccccaag 3540

ctgagagcca ccgaacagat gaccaatgtg ggctgcgcct tcagcggccg gattctgcat 3600

caacctgaaa tggccgccat gcggctgatg cccaagttcc tgaatagcac caacagctgg 3660

tggacctgta ccaacctgag cgtgcccatg atgctgacca ttctgatttg gaagcgggtg 3720

ttcattctcc tgctgtccga caagaagctg ctgaagaagt ctagccagct gaggcggtct 3780

tggagaaacg tgtcccggat cagccaccag atgaagtgtc tgaccgccgt ggactgtctg 3840

caggccttct gggacatgaa catggaacac cggctgacca tggacagcgc cctgagcctg 3900

acaaagagag agggacccga gacttgcaag aagaagctcc tgctgaaact gctgacaatg 3960

agcctgagca acatcctgat gaagttccgg ctgcggctga gaatcccaag cctgaggaag 4020

tatcgccagc atctgaaaaa gaccagcgcc aagggcgaga agctgaagca ctgctttaag 4080

gcttggggca gaggcccacc tctgcacaca agaagccaga agaaagaccc tgccggcaag 4140

ctgaaaaaac tgaccacact gagcttcgtg ctgatcgagg aacggcggaa cctgcagcgg 4200

aatctgaaag tcctgtggct caagaaaagc accaagagcc ggtatctgtt cttcctgacc 4260

gtgtgcggct tcttcgagaa gaaacggcct gagtggctgt cctccagaaa gaagggacac 4320

acaaagaatc ggaggaaggc caagcggaag ccccagatcg tgcacaagaa gagaggcctg 4380

ttcctcaaaa agaaatacct ggtgcaggggc gccaagaaga tcaccttcct gtccaacggc 4440

atggaagctt gcggcacaca ccccacacca agactgagcg acagagtgtt cctggtcaag 4500

atcaccacca ccacaagcag ccccagaaga agcccagcac tgagagctag aggcggcacc 4560

acaattggcg aagtgaccag cctgtgcttc ttctacctgc tgtacggcta cccctacgat 4620

gtccctgact atgcgtcc 4638

<210> 1126

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 4A poly-FSP

<400> 1126

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaatc cagaagaaga aacagaaatc catggccaag 120

180

cacagacaga gaaacaacgc caagaagatg cccttccaag aggaaaagaa gaacggccgg 240

atcagcagca gcctgatcct gagcttccag agagtgaagg acagcaagaa aatgacccag 300

aagatgtgca gctctctggc cctgcctaca ggcctgacat ctctgatcct gcctagcagc 360

gatctggccg tgctgatctc tagcagcacc agccactttc tgatgcggag ccctgtgctg 420

ccaagctcca gactgacatg tgcctctcca cagctgcccc ggatgtggac atggtctagc 480

tggctgaagc accccagaac cgctagcatc acactgccta ccacaagcct gaagggcagc 540

agaaccagct gctgcccaag atctcacccc agactgccta ggaccaagtg gcctctgcag 600

tgtctgctgc acagcctggg aacaacaggc acactcgttt gggaaagggg cgtgctgcag 660

atgagaagcc tcgaagccca ttgtcctctg cagaccttcc atctgcacca ctgcagaacc 720

cagccaagct cttgtcctcc tgcctggatt ctgtaccaag gccagtggag cggagccgtc 780

gttggactta gacccgtggc tcaccatctg cctagaccta tccagggcct gcactgtcct 840

caggctcctc atcaacctgc tctgagctgg ggaaagtggc ccgtgggaat gacaggacct 900

gccagatgct gtcggtggcc ccgcagatct cagaagtctc agcatcactg cgccctgcgg 960

aagatcgagt gggccacaaa actgtctctc ggcggaaaag ccggaccaca ggctcaagag 1020

aaggccagac cttctccttg cctgtcctgg tccagcagaa ttgtggcccc tggacacgcc 1080

ctgagctgtc ggactcgtag aagatggtcc tccaagtgca gaggcggccc tagcgccagc 1140

gatagatgtt gtagctgtag ccccagcggc ttcagcgccg gaagaggcag atgtcctgtg 1200

cagggatgtc tgaggccaca cagagttcag ctgctgagaa gatggggccc aggatctcct 1260

gctggccaga gactgtctaa gggcttccaa ctgctgcgtt ggtggggacc tggatctcca 1320

gctcctgagc ctagaaaggg cccatttcct ccacctgatc ctccatggcc tgtgaccgct 1380

gtgacagtga tggctggctc tgtgcctagc gcacagtctg tggatgccct ggaatctcct 1440

ggacctctgg ctctggaagg ccctagcagc cctagaaacc tgctgtggcg ggaaatgagc 1500

atctttctgc ccggcatctt ctgccaaaag aaggtgccat ctgccagccc ctggggactt 1560

gttcctcctt ctccaccact cgctctgggc cctagaatgc agctgtgtac ccagctcgcc 1620

agattcttcc ccatcacacc tccagtgtgg cacatcctgg gacctcagag acacacacct 1680

caggtggtcc tgagactggg caacctgcct ccattcttca gaggcagcga ggtgcccttt 1740

cctagacctt gccggaccaa gaacgagggc aacaatcact ggccgagt ggaaatgcct 1800

accggctggc tgctcgtggg ctacaacacc agaaacacag cccagaatcc tcggctgcag 1860

cagcatctga aacactggca gcagtgcaca cgggccagat ggtgtcaaga gccttgtcag 1920

accctgacct tcttccactt cttcttcttt ttcaaaacgg tgtccctgtg ttgtcccggc 1980

tggtccaaag gacaacctcc acctcctggc ctgaagcctt ctagcctggc ttctcaggtg 2040

gccagaacca caggcgtgta ccagcacgtg tggctgatct gcttcgtgtt cttcgtgtgc 2100

2160

ggcatcaccg acgtgaacca ttgggcccag cctaagccat ctcctagcaa aggcaccggc 2220

atgaacagac ccatcggcct gagagttggc aaaggcgtgt gggccagact gctcaagaag 2280

aagcctagag acatctgtcg gatctccaac agaaagaccc ggaaggccgc cagagagaag 2340

caccgcaaaa cagatagagc cagcgtgtcc ttctggactc tgctgcctac aacaggcgtg 2400

cggacaagag gaagccctct gagactgact ctgtggcctc ctagatgcag atgcagacgc 2460

gagtacagag tgaccatgaa catcctgaac agcctgccaa gcagcatgga aatcgccgtg 2520

ctgcaagaaa ccgccaagaa aagccctacc agcttctgct gcaacctgga cctgcagcat 2580

cagcgcagac gcaaaaagaa gaaagacaag gtcgagatcc ggaatcggag ccccaaggcc 2640

aatagcacag agtgtcaggg cgagcacagc agagagcaga atctgctgaa tctggtgcag 2700

ctgaatcctc acagccctca ccaccggaag gacgaagagg accaccagaa gagacaccac 2760

caccacaatc aaaagaagat gagcgtccca ctgttcttcc acggcgacct gtgtagagag 2820

agcaccctgg gcaatttctg gatgaagctg ctccagctgc ctcagaggcc tccactgcaa 2880

ccagtgaccg gaatggaaat cctgctttgg attctgctga agatggaaat tcagatcttc 2940

aagatttgcc tgggcgagat gcagctctgc acctctcgga ttctgaacct gcaagtgctg 3000

tatgaagctg taccacagcc tcgtgatgtg cctgcggctg 3060

aggaccaagc tggaaaaggc tctgagcgcc ctgttcatct ggcctcagca ctcctacaag 3120

atcacacacc cattcgccag ccagccactg ggacaagaga tgcccaacaa cacccagatc 3180

3240

ggcagcttct ccgccgattt ttcagcccct caaggacagg cttttggctc cctgtgtcag 3300

ctggtgtccg ccgactttag cgccccacaa gtgaccagac tgcggagcct gaaccatctg 3360

ctgatttggc tgaagaattg gaagcggttc aagctcaaaa agaaatatca ggcccctctg 3420

cggctgtggt cttggtgcgg aacaagccag acctacttcg gcagcatcat ctgtatcaag 3480

aatatgttcc tgcagcggaa gggtttcctg aacctgagcc acgtggaaaa gttcgagctt 3540

aagaagaatc ttctgctccc cgtcgtgatc aacctgaaga gactgcagaa attcatgctg 3600

aaccagaagt gcctggctcc atttcctgcc tgcaccagca aagcctggac acccagaaaa 3660

ctcgtgggca gagccgtgcg gagaaagggc attctggcca tgacactgac cctgaacgga 3720

atcgaggccc tgacagccct gtttacctgt agaaccgcct ccatcagcgt gaacatcagc 3780

accctgatga gcatcctgga agccatcatc aagaagatga agaaacggat catggtgcac 3840

cggtggatct tctccaatca gctgaccgtg cagatgctct gcctgcctct gctgacccct 3900

gacaccagat ggatctttag taatcagctg cagacagtgc aaatgctgtg tctgccacct 3960

ctgaagcagg gcgagagaat catttggcca ctcggcggct ttcagatccc caatttcttc 4020

tggtccctcg gcatcggaca cactggcgca tggtggcata ctggtagaag gcggagagag 4080

tggggcggct ctaccgtgtc tagaagccca ctgtggatca gctccatgat ctacctgaga 4140

ggcatggcct tcgccagcga cagctccatc agaaccacca gagtgtcctg gtgtcggaga 4200

tgtggcgcca agtctgccgc agcctcttct tctccactgt gggattgcag caccacaacc 4260

ggcacacacc acggatgcct gtgggagatc cctagcgtta ccctgctggc taacctgcac 4320

ctgtgctact accactctca gagcaacaga aacaagagcc gccagatgga atccctgggc 4380

atgaagctcc aacttaaaaa gaaagtgaac gcctgcagca cctacacacc ccacatctgc 4440

agacacacct gtcgcgtgaa cgccgtcgag gaactgcact ctagaaggat gtccgtgcgg 4500

atcgtgaaaa attgggtgtc ctgctgctac gtgcggctgt cagctgtggg actgtctact 4560

tggagcgctc tggacaacaa gatcatctgc aagggccaca gaatcaccgg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1127

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 1B poly-FSP

<400> 1127

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaacc gagatccaat tgaccatgaa cgacagcaag 120

cacaagctgg aaagccccgc tctgaaacag gtgtcaccag cctctccacc tactcagcag 180

cctcagacac ctcaggatag cagacaggtg ctggccatgc ctagcagctg gtctagctct 240

ggcagaaccc agcggaaggt ggtcaagaat cctagccagc ctgtgcctct gagaaacgac 300

ggcatcacca acggcaacga ctccaccaga agctgcagca tcagcctgaa gaaaagcctg 360

gaaaagatgc ccctcatgat gaagaagaag gacagcacca tcttcatcgt gcccatgagc 420

ctggcctgta gcatcaccgt gtctttcgtg ggccagatca gcacactggt gctggatgtg 480

tccagcatct cccactggaa caaagtgcgg cagaacttca agcggaacgc cgacaagaag 540

aacgttcgga gcggcgagaa gaacgacctg cagctgtttg tgatgagcga caccacctac 600

aagatctact ggaccgtgat cctgctgaac ccctgcggca atctgcacct gaaaaccacc 660

agcctgggcg actgtcctag aacaatggcc ggaccttctg cctctctgca ccctgcatct 720

ggcagagcct ctagaaagac ctggcagaga aggctgagag cctgtagagc tggtggacct 780

gctggatggg ccagacaggc cttcgataag aaaggctttg tgatcctgag cgtcggagcc 840

ggaacaggcg gaggacctgc ttgtgttcct gtggctgaag cttctacagg cgcttggaca 900

gctgaaggcg ccgctggaac aagatgtaga gcttctagag ccccttgcag acagctgcct 960

gccagctctc ctaccaagtg cgagatctgc gtgaagcgca gacggcggag aaagagaagc 1020

agccagtcta gaagccccgt gcctaagaaa cgcgagagaa tgatgctgcc cgtgcgggcc 1080

agaacagcta gatctgcact tcctcagggg agagcccctc ttgagggaat gctgcttcct 1140

agagcactgc ccagcctgct gtctagaatg ccagccagaa gagagagccg gttttggctg 1200

gctctgcagg cacttggact cagacctcca catctgccta gctgtcatcc tctgcctcaa 1260

ctgcttggcc ctccacctcc tcatcaggcc cctacagctt tcttcctgct ggacatcatt 1320

ctgcggctgc cctgcaaagt gaccgagtct agcaatatgg gcggcaacag atccatctgc 1380

ctgaccaacg ccaagaaaat ggaagatatg atctgggaga gcacgcccct gagcatcagc 1440

agacctacat ctagagccag gcagagctgt tgtctgcccg gactgacaca tcctgctcac 1500

cagccactgg gatctatgac cctggccatc agattcatca gcgctcctca gcctagctcc 1560

agcacagtga cagctgctcc tagcggccag tacttcaagg gcaagaagcg gcggaaagag 1620

atcttcaaga agaagaaacc cctgacattc agccccagcc tggccaaaaa gaccctgacc 1680

aatagcagct tcttctacac cagcgaccgc gaggccaaga aatctatgaa gcggagcatg 1740

accctgaagg ccctggtcaa gacctggaca cagttcagaa actaccccag agcctcctct 1800

cctggacacc ctctgggacc taatggcatc tgggaagtga tgcccagaac actggaaggc 1860

tgcatctccg tgaaggacca cagaacaagc cagaaccaca gcaagtgcat caccgagtgt 1920

gtgcggacag ccctgaacag aagaagcaag atcgaggcct ctcctatcgg ctctatcgcc 1980

ctgagatcca tgcctaagag cagaaaccag gtgctgttca gacggaatct gaccccaagc 2040

cagctgagaa ctctggcccc tccttatatg ctgctgcccc agtctctgag ccagagcctg 2100

agcagaaagc agatccctag ccagtccatg ctggtggcct ctagagcagg acagtttcct 2160

2220

cctctgagat gccctagcag aagccctgcc acattcctga cagctctgaa cggctggcac 2280

agatctggac tgagagccca gagaaagcgc aagcgcgaga agagaagcca gaagtccagg 2340

cagaaccatc tgcagctgaa gtcctgccgg cgcaagatct ccaactggtc caagctgaga 2400

agcccctgga tcttcgtgtc caagaagaga ctgcccaagc tgaccaagtg tgaaatctgt 2460

gtcaagaaca ccgccatcct ggctatcacc ctgatcaccc ctcaggaccc tcatctggac 2520

cctgtgtgtc accctcatag aaggcctgga tggaagggcc accacagagt ggtgttttct 2580

catcacccta gcggcagcac caagagccac tggcagcaaa gagaacgcgg aggcggcaga 2640

cgggaaaaga agggctgtag agagagatcc ctgctgtact ccagaatgtg cctggtgcag 2700

atccccacac acacctggga gcccgattac ttcgtgtctc tgcccatgat gttcagctgg 2760

gccacacagt cattccccgt gggagccgtg tttctgacca gacctgccat tccttgctgt 2820

agctgcacaa gccacagcaa cccttggagt cagagcagac acccacacag aagcccacct 2880

acctcttgtc ctccgctgct ggaagccttt ccactgccta gagcatccct gagagccacc 2940

gtgccaagac atagctctgt gctggcaacc ggactgcatc tgcacagaca gatttctctg 3000

cccctgagag gattcctgct gcatcaacca ctgccaggcc agcatctgtg gctgtctcat 3060

agactgcggc tgagactgcc aagaactcag cggcctccta gatccatcct gagccttaga 3120

ctgccactga ggccacagag aggacccaga cctctgcata gatggctgca gcagaccaca 3180

ggctgtagcc agagaagata ccccagactg aaaaccttcg gcgtgccact gggctccatt 3240

ctgtgtctgg ctggatctct gagcaccatg atgatcatta gcaccaccag acaagagtgt 3300

ggcaggcggg catctgtgcc ctgtagacct atgatcggaa gcgccagacc aggaccttgg 3360

agaacatctg ctatgcctag cgccatgggc gtcgcactgc ctacaagctg tgaatccgga 3420

agatctcctc ctgccaccgg cggaagaatg cctggaagcc agagcattat gatgagctgg 3480

atgcctcctc tggctccttg tgccgcttgt ccttgttctc ccgctcctac actgtgtccc 3540

gctcatcctg ctagagcacc tacagccgtg cctgccttta cacctctgtc tgcacatcct 3600

gtgcctgtgc tgtctggatg tcacctggcc gtcagaacct ccatgctgac actgctgcct 3660

atgctgaagc tggccccact gcaagtgacc accacactga agctgatcct ggtcatgctg 3720

gaaatcgtga ccgagagcac tcggagagag atgccttctc aggccacacc ttctccacct 3780

ggaccttggc gagccgctct gggcaaagaa gtgaagagat cccagagcaa gctgcgcctg 3840

agaaacacaa gccccatcgt gttctttctg gccccatact tccccagacg gatgaccaga 3900

3960

aggcggagaa tgacaatgat gacaaggcgg accctgctga caaagaaaac ctgtctgacc 4020

cctagaacca gcgccgctac caaagctctg ccaaggcaga caatctccca gcggaggcct 4080

attcagaccc ctcctggaac atctctggcc atctggggca accagcagct gaaatggacc 4140

cctcacatcc tgaagtccgc cagcagaagg gccagaccta ccagctgtag cacctgtaga 4200

atgtggtgga tcgccaaaaa gcggatcaag ctgcggcggga acagaaccag actgacactg 4260

acaggcacaa gaagcagcgg caccagagct tctccatccg ccgtttgcct gtgttgctgg 4320

acctgtcctt ccagaagctt cccagccgct tctccaagcg gattgagagc acctgttgga 4380

gcacaggccc ctccagaagt tacactgaga gctagctgtg cctccagcgc ctctaaggct 4440

tgtgctggac tttggccttg gggagcacaa gttcggcaac catctttgcc tggaccatgc 4500

gcttctctgc tgagcacact gtctcagcca cctccacagg ctccatctca agtgtggaca 4560

gccgcaacac tgagatgtcc tgctgttcca gctgctgcct gtcctcctgg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1128

<211> 4623

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 2B poly-FSP

<400> 1128

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaaga aagatgcaga gcctgaatca ccccagcaga 120

ctgaccaaca tgagcagaac acctccaaga cgcgtgtgca accctcggag aaagacctgg 180

aagatgcgga agaagtacgc ccagaagcct agccagaaga accacctgta caacagccgg 240

aagaaactgc tcgaggacat gaagcagaat ggcttcacag gccacagaga gggcagcgag 300

tctgataatc tgggcctgct gtactccttt ccctcctatcc ggatgtgcgc cctgtctcac 360

cctagcttct acatcagcag caccgtgtgc tttctgagca agcactgcat gaccgcctgc 420

acacaaaaga aaggccacat gatcttcagc cgcgagaaga tcatcaaccc caccatcagc 480

tgcccctttc agagccccac aaagcggctg gaactgagcc tggaaccttg gattccctac 540

ctgcaccagc agaaaaccca aggcggcaag aagagaagca agttccctgg caccagcctg 600

cagtgctacc tgaacagatg cagcctgatg gaacagatcc ctcacctgaa gtccgcctct 660

ccacagatca tgcagagaac cagcgccgac cggttccgga tgaagcggag aatgcagaac 720

ctggacaatc tgggcgttcc atggccactg ctgcctggac ctctggaaaa cacacagatc 780

cccagaacaa gccctctgtg gaagatcaca atggtgctga cataccccaa ctgcgtggtg 840

cacgtgctga tgttctggct gcctatgaga tctctgggcg agccttggag agctggcttt 900

cagtggggag agtccccttg gcaagtgatg gctagcctga caggccccta tcctatcagc 960

gagggcctga ccatgaccac cggcagaaac atcaacagca actccctgat gaccaactgc 1020

ctgttcagcc ccatcttcct gtgccaccac gccatcatgc accggaagat gacaagccca 1080

cacttcagac tgttcagcag caagatccca catcctcagg tgccatctgt ggtggccctg 1140

tgcaagttca gagccacatt tctgctcgcc ctgtgggagt gtagtctgcc tcaagccaga 1200

ctgtgcctga tcgtgtctag aaccctgctg ctggttcagt ctgccgctcc tccacataga 1260

ctgctgggcc cttctagatg gtccagaagc cacctggact tcagccccta ctggatgaag 1320

aaagtgaagc tggccaacgg cctgtggttc aacctgatct tcgtgctgtt caaggccgac 1380

cagagcgagt ctagcctgtc tagcggccgg aacaagaaaa caaagctgtg gttctccctc 1440

atcaacatcc accaccgcaa gaatcctctg ctgcccatga gaatccccag cctgaatgcc 1500

cagtggatgt tcctgagaat cctgcttctg gcccatatgt acggcttcag cacctacggc 1560

acccagaaag gcgagtgcgt ttgtctgcag tgtctgagcg gcagcagcct gttcattctg 1620

aagcagcccc tggacctgaa gatctccaag accttcatgc tgcaagctca cccacaggtg 1680

ccagctcttg ctcctcaagc ttggtggcct gctagaagag gccctctgac atctgctcca 1740

tccgctcagc agtctctgac agctagccct cagattatgc agcggatgag cgccgtgaac 1800

ttcgagacaa tcatctaccc tccaagcacc tgtagcctgc cttgtagagg cagaacactg 1860

gaaagcgccg gactgtttcc ttggctgcac acccagtgta cacacccctg taccatgctg 1920

ctgccacaag gctggtggtg tacaagaggc ggcggaaagc ggaaaaagaa aatgtgcaag 1980

gtgtccggcg gctgcaccgc ctgtgttgag agatgtcctt ggaagggcat ctgctggaag 2040

tggccagtga cagtggctgc tcagtggtct agcgtgacct ctctggaatg gacagcccaa 2100

gagagaggct gcagcagctg gctgatgaag caaacatgga tgaagtcttg gtccctgcgg 2160

gaccccagct acagatccat cctggaatac gtgtccacca gagtgctgtg gatgcccaca 2220

agcaccgtgc ggagaagaaa cagcagaaag cagtctcagg tgcagctggc cattcaggtg 2280

ctgctcgtgg acaagcacaa gtactttctg ttcggcagag cccgcaaccg gaacatgaag 2340

aaaaccatga gccacctgat cgcccatcag gccctgaaga tgagcggcct gaggctgttt 2400

catcctcggc tgctgacaag aaagaagccc ggcaagttcc tgcctagcag atttccttgg 2460

ggccctcctc ctctgtgtca tcctgcttgg ctgagcccta tctccgcctg tccaagactg 2520

ctgccatatc cttttcacgt gctggccctg gaagtgacct ttccacctgg aacagccatg 2580

cctcagatgc aactgcaggc cctggccatg tgggagaaag gctgggataa gcggaagaga 2640

atgaagaccg gcgccatcca agagaagggc gctattcaag agaaaggggc catgaccaag 2700

cggatctgcg tggtcaattt cgaggcctgc agagagatgg aaagcctgag cgcccctgag 2760

aagattaccc tgtttgaagc ccatatggcc cggtgcacct ccatcaatgt gctgtgtgtc 2820

agagcctctc tgatcgagaa gctgatgaaa gaaaagcgca agatgaagcg caaccaggtg 2880

gcatccccac aaattatgca gaggctgttc attaagggct accagaacaa ccacttcacc 2940

ttccatctgc aaaacttcgc cttcttcagc agcttcggca acaacaagta catcaatatc 3000

3060

ggcatcttca cctttcggtt ctgggtcgaa aagctgagct ggaacatctg caagaacctg 3120

agcctgtacc tggataagtt cgtgcagcgg ctgctgtacg tgtacatcat cgagacaaac 3180

gtgttcgtgc tgaacctgtt ccacgtgcca gtgaaccaga tcttcatgat ggccttctac 3240

ctgaagtaca ccgagaaggc ctgggtcatg gaactggaat ggctgatcca acagcagtgt 3300

taccagcaag agaagaaaag ccggaaactg atgagcagct gccgggcctt catccacaca 3360

cctagaagcc ctggctgcag cgtgtacgcc aatatgtgct gggccttttg gaagcctcct 3420

agccagggca tcagctcttg tctgatgaca ggctgttggg gcagccctcc tctgcacctg 3480

tgtcagcctc tgaggcggta taagaaaagg ggcgtgatcg cctggcggcg gagaaagtgg 3540

cagatccggc atgtgctgaa aaacctgatc ctgtttcatg tgcggaacat cgtgtacggc 3600

atccaagtga tgctgtgcgg caagggactg acacaccctg tgcctctgaa acggaccctg 3660

cagaacaaga ggaacaagaa catcaagcgg gtcaaccaga agaagggcaa catcaagagg 3720

aagtccatcc tgctgcacct cattgtgctg aatagccccg agagcaaccc caagttcttc 3780

ctgaccttta gccagctcga gaagaaagct acctggatga gaagaacagg cgccctgaga 3840

gccacagctc ctgctacagc agctcctcag gctcagactc ctcattggag agccgccaca 3900

acaagcagca tgagcagcag ccctagagcc gagcctacat cttctagctc ttgggccacc 3960

tgttggccca gatgctgtag ctccagctgt tactggtccc tgagttcttg gcctcctgct 4020

ggcgccgctg aggctacaaa caccagaact agaagcagag agtcccagcg gggcagaatg 4080

ctgacttgga gaagtagtct gtggctgcag ggcaccagat gttttacagg cgtgcggaca 4140

agcagcggaa gctgtggcag agcttgtagc tgcggaagag gctgtggaac ccctagaggc 4200

gattggaaga gacaccctcc tcggaggaat agcgtgatcg tgcacaagat cgtgctggtg 4260

tttctgaagt ccctgtccag ctacaactgc gagagaagga tcagcggcat ccagaagaga 4320

gccatcgagt ttgccgtgct gaaactgctg aatatggacc tgcactggaa ctccgcctac 4380

ctgggcagaa ctctgctggt cacctgtatc ctgtatcacc ggtggatcgt gacctccatg 4440

tgccagtctc agagatggct gaccagcacc acaagcagat ccagcgctct gatgtggacc 4500

cggcagatca atcggaggaa gctgcagaga aagaagaggc acccaagctg gccttggacc 4560

agatgcctta gaatgaggcc tcctagaagc ggctacccct acgatgtccc tgactatgcg 4620

tcc 4623

<210> 1129

<211> 4638

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 3B poly-FSP

<400> 1129

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgagga gctgctgctg cctcttgtca gtctcctcct 120

tctcctcggg aaaccacacc tagctccgac gccaccagac ggacaagacc aattgccgct 180

cagagcaaga aaaccatgac agccgccgga ctgaccccta agaacaagaa gcggagaaag 240

ctgcggccca agatgatccg gaagatccag aagagaaggc ggaccgccaa ccggcggaat 300

agcagaaggg ctacccctac aagcaagggc agcctgagaa gaaagtacct gagagtgcgg 360

ggacctcctc tgcacacccg gaacatcaga aagctgatct ggaccaagca gcggagcttc 420

ctgtctctgt tccccaagct gagagccacc gagcagatga ccaatgtggg ctgctctatc 480

cctcacgtgc ccaaagccac agccgctcac tctgtgacag tgaaactgca gctgcccctg 540

accttcagca ccctgctgtg ggatagcaga cacaccatga gcctgagcaa catcctgatg 600

aagttccggc tgcagtctag cccattcctg gccagatcct ggaacaccat gcagtggaac 660

aacaagaaaa tggccatgac cgtgatcgct ggcgcccacc actgtcagta caagctgacc 720

tacaccaaag tgtggaagat gaactggaag aaaatgatga tcggcagcca gcatctgctg 780

ttcagccctc tggatagcag tcccctgcag ctcctgctgc accctcacgg caagagatgc 840

aacccttgct gcagactgac ctggatgagc cagaacacca gaagcaccaa cctggaaaag 900

aaccccagcc tgaagaagca gagccagcgg agaggaaagc tgagcgcctt tagcggcaga 960

atcctgcacc agccagaaat ggccgccatg agactgatgc ccaagttcct gaactccacc 1020

aacagctggt ggaccctgac cacactgagc ttcgtgctga tcgaggaagc cagacagccc 1080

agcagaccta ccggcagaac acctaccacc agactgatgg cccctgtggg cagcgtgatg 1140

agctgttctc tgctgaccca gccttctcca gccagcgcct gtacaatgcc tccactgctg 1200

catccacagt gggcccctca tagtgctgga ctttctccag gcgcttgcag acctgcctgc 1260

acctgtatca gcatgaccac catcagcagg gccacatggg gcccagtgta ctacgtgtgc 1320

aaccggatga acgtgtccag cgtgctgaag cccttcctgt tcaccgtgcc tatcgaggac 1380

atgagagagt gcaccctggg cagaaacaga atgaatgtgt cctctgtgct gaaaccctgt 1440

ctgatccctg tgccaatcag cgagaagaag aggaagctgc ccaacatcga gatcacccct 1500

cagcggtcta aggccatgct gcagaagcag tgctgctaca acctcgtgat caagcaccct 1560

ctgatcttca gaatcaacct gcggcggcag ggcaagacca aagatggcgt tgtgcagagc 1620

ctgggcttcc tcaaccagca gatcatcctg cagaaagagt tcttcatctg cctgctcatg 1680

ttcatcaagc ggtggaagcc tgccgccacc aagagcagaa agaagggcca caccaagaac 1740

agacggaagg ccaagagaaa gccccagatc tacctgttct tcctgaccgt gtgcggcttt 1800

cacctgaaaa agaccagcgc caagtggctg aagaagtcca ccaagtccag atccaacggc 1860

atggaagctt gcggcacaca ccccactcct agactgagct gcctgagagt tagatgcggc 1920

ggctggctgt gtggctggac acctagaggt agaaagtccg gcgtgatgaa gtgctgggat 1980

ctgtgccccg tgcacaagaa gagaggcctg ttcagactgg attttcaccc cagaagcagc 2040

tccggcaagg gaaagaacag cccctgctgc caaaagaaac tgcgggtgca gaaccagggc 2100

catctgctga tgattctgct gcacaatgac cgggtgttcc tcgtgaagat cggcgagaag 2160

ctgaagcact gtttcaaggc ctggggctcc agacggaacc ggcggtacag aaagatctac 2220

ggcgagctga agatgtccct gcaggccttc ctgatccacc tgagaaaggt ggacacccac 2280

cacctccagg tggtcaccaa aacagtgacc gccacaacag ccgacggcca gagaatgcgg 2340

agactgaagt gcaaggcctg ccggctgaga tgggccagac ctgaacctgc tgctcaggcc 2400

cctcctaaga gccactttcc acctcatcct agcggcccca gcagaacaag tagcctgcag 2460

ctgagaagcc tgttctttag ccactgcctg tgcagccaga agaaggaccc tgccggcaag 2520

ctgaaaaagc tgctgaagaa aagcagccag ctgcggagaa gctggcgcaa tgtgtccaga 2580

atcagcaccc ctcctccact gatcctgtgg cctcaagctg ctcctgtgtc cgtgatcacc 2640

atgaagctgt gggagaagca cttcaagggc ctgaattgga acctggtcgt gtggattctg 2700

aacctgaaga tgatgaccta catggaaatg cactacggcc atcaggcccc acaggtggca 2760

atgctgccta agctgctgtg cagacagatg ctgcccactc tgagcacact gcagcagaga 2820

catcctcagg gcccttgtcc tcctcctgtg acacctctga gagtccagtc tgtcctgctg 2880

ctgggagtgc ctcagacagc cagaggacct agaagaaagg tcctgatggt gctgtggctg 2940

cccgccaatc agtctctgca gaaaagccag gttttccagt gggtgctgcg gacagaggtg 3000

gcccctagat gtcctagatc tgccgtggac atcagccccg gacctacatc tcctagcctg 3060

3120 gcctcttgtg cctctgtggc cattggaaag

ggtgctgctg gcgctagaga ggccggaaga tttgctgtgc tgggcaccat ggtcatgaag 3180

ctttgtttct tctacctgct gtaccggctg cgcatcccaa gcctgaggaa gtacagacag 3240

cacctgaacc ggaagctgca cctgacaaga tcctgcctga tcgatctgac catgatgatg 3300

3360

ctgctgccac agtaccctct gcacccacct ccaccttgtc tgctgttgct gcaccataga 3420

caccacctgg ccttttctcga gatggcctgt agctctcagc acacccacag catcaagatc 3480

tggatcagct tcagccatga agtggaccct ccaggcagcg tgtggatact gccttgcgat 3540

ctgcctcgga tgctgccaat ctcttcaccc ctgcacagac cccagatgcc tttctggacc 3600

tgcacctttc ctgccacaac ctggggcaca tgggagacac cttctacatg gggctggcgg 3660

actttcagag gcgctagcag cagcacatcc cgggacagca gatccttctt cacctctgct 3720

caccctagca ggccctgcag aatgagactg taccagtgca agaagaagct cctcctgaaa 3780

ctgctgacca ccaccacaag cagccccaga agatcccctg ctctgagagc aagaggcggc 3840

accacaatcg gcgaagtgac cagctgtacc aacctgagcg tgcccatgat gctgaccatc 3900

ctgatttgga agcgggtgtt catcctcctg ctgagcgata agaagggcgc caagaagatc 3960

accttcctgc ctattcctcc acacctgaga caccctctgc tgctgttcga gaagaaacgg 4020

cctgagtggc tgagcatgga cctggacgga atgctgacag tgatcggcca gggactcgtg 4080

ggcatgaccc ctagagatct gggaacagtg gcaggcgcca ccgctcagat tcagcgacct 4140

atccaggtgc agactggcgg cagactcaga agaaacctgc agagaaacct gaaggtgctc 4200

caggtgtccg tgtacagcgg caacctgctg tcctttagca gacggggcat gaagtctagc 4260

gtgcggctga aaaaccgcga gtgggagaaa cggaagcaga ttctcgggagg cggcggaaag 4320

tacaaagagt acttcctgaa gcgcatcctc atccggaagg ctatgacagt gctggccggc 4380

gacaagaaag gactgggcag attcatgaga tgcgtgcaga gcgagacaaa ggccgtgtct 4440

ctgcaactgc ctctgggaag aaacatggaa caccggttga ccatggacag cgccctgagc 4500

ctgacaaaga gagagggacc cgagacactc aaaaagaaat acctggtcca gcaccagatg 4560

aagtgtctga ccgccgtgga ttgtctgcag gctttttggg acatgggcta cccctacgat 4620

gtccctgact atgcgtcc 4638

<210> 1130

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 4B poly-FSP

<400> 1130

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaatc cagaagaaga agcagaagtc caacctggac 120

ctgcagcacc agcgcagacg caaaaagaag aaggacaagg tcgagatccg gaatcggagc 180

cccaaggcca atagcacaga gtgtcagggc gagcacagca gagagcagaa cctgctgaat 240

ctggtgcagc tgaaccctca cagccctcac cacagaaagg acgaagagga ccaccagaag 300

cggcaccacc accacaatca aaagaaaatg

ctcggacagg cctttggctc tctgtgtcag ctggtgtccg ccgatttcag cgcccctcaa 420

gtgaccagac tgcggagcct gaaccatctg ctgatcaacg agggcaacaa ccactggcct 480

agagtggaaa tgcctaccgg ttggctgctc gtgggctaca acaccagaaa cacagcccag 540

aatcctcggc tgcagcagca cctgaaacac tggcagcagt gcaccagagc cagatggtgc 600

caagagcctt gtcagaccct gaccttcttc catttcttct tctttttcaa aacggtgtcc 660

ctgtgctgcc ccggctggtc taaaggacaa ccctcctccac ctggcctgaa gccttctagc 720

ctggcttctc aggtggccag aaccacaggc gtgtaccagc acgtgtggct gatctgcttc 780

gtgttcttcg tgtgcgtgga aaccggcttc tgtcacgtgg cccaagtggg actcgagaga 840

ctggtgtctc tgagcggcat caccgacgtg aaccattggg cccagcctaa gccatctcct 900

agcaaaggca ccggcatgaa cagacccatc ggcctgagag ttggcaaagg cgtgtgggcc 960

agactgagag cctccgtgtc tttctggacc ctgctgccta caacaggcgt gcggacaaga 1020

1080

agcatggaaa tcgccgtgct gcaagagaac cgcaagacca gaaaggccgc cagagagaag 1140

cacagaaaga ccgacaacct gcacctgtgc tactaccaca gccagagcaa cgggaacaag 1200

agccggcaga tggaaagcct gggcatgaag ctgcagagcc ctctgtggga ttgctccacc 1260

acaacaggca cacaccacgg ctgtctgtgg gagatcccta gcgttacact gctggcctgc 1320

1380

tgggtgtcct gctgttacgt gcgcctgtct gctgtgggcc tgtctacttg gagcgccctg 1440

gacgtggtca tcaacctgaa gcggctgcag aagttcagcc agaagtctca gcaccactgc 1500

1560

gctcaagaga aggccaacgc caagaagatg cccttccagc ggtggatctt cagcaatcag 1620

ctgaccgtgc agatgctgtg cctgcctatt gaagccctga ctgccctgtt cacctgtcgg 1680

acagccagca tcagcgtgaa catcagcacc ctgatgagca tcctggaagc ccctctgttt 1740

tttcacggcg acctgtgcga ggaaaagaag aacggccgga tcagcagcca ggctcctctc 1800

agactttggt cttggtgcgg cacaagccag acctactttg gcagcaccgc caagaaaagc 1860

cccaccagct tttgctgcga gtggggagga caggctcacc ggcagagaaa taccggaatg 1920

gaaatcctgc tgtggattct gctcaagatg gaaattcaga tcttcaagat ttgcctgggc 1980

gagatgcagc tgtgcaccta cacaccccac atctgcagac acacctgtag aggcggacct 2040

agcgccagcg atagatgctg tagctgtagc cctagcggct tcagcgccgg aagaggcaga 2100

tgtccagttc agggatgtct gcggcctcac agagtgcagc tgctgagaag atggggccct 2160

ggatctcctg ctggccagag actgtctaag ggctttcagc tgctccgttg gtggggacca 2220

ggatctccag ctcctgagcc tagaaagggc ccatttccac cacctgatcc tccatggcct 2280

gtgaccgctg tgacagtgat ggctggctct gtgcctagcg ctcagtctgt ggatgctctg 2340

gaaagccctg gacctctggc actggaagga cctagcagcc ccagaaacct gctctggcgg 2400

gaaatgagca tcttcctgcc tggcatcttc acctctcgga ttctgaacct gcaagtgctg 2460

aagaagatcc tgcggagctt tatgaagctg taccactctc tggtcatgtg cctgcggctg 2520

cggaccaagc tggaaaaagc tctgagcgct ctgttcatct ggccccagca cagctacaag 2580

cagaggcctc ctctgcagcc tgtgcaggtc gtgctcagac tgggaaacct gcctccattc 2640

ttcagaggca gcgaggtgcc ctttcctaga ccttgccgga ccaagatgct gaaccagaag 2700

tgtctggccc ctttcatcac acacccattc gcctctcagc ccctgggaca agagatgccc 2760

aacaacaccc agatcagact gctgaagccc ctgctgaccc ctgacaccag atggatcttc 2820

tccaaccagc tgcagacagt ccagatgctc tgtctgccac ctctgaagca gggcgagaga 2880

atcatttggc ccctcggcgg ctttcagatc cccaacttct tttggagcct cggaatcggc 2940

cacactggcg cctggtggca tactggaagg cggagaagag aatggggcgg ctccaccgtg 3000

tctagaagcc cactgtggat cagctccatg atctacctga gaggcatggc cttcgccagc 3060

gacagctcca tcagaaccac cagagtgtcc tggtgcagaa gatgcggcgc caaatctgcc 3120

gctgcctcca gcctcaagaa gaaaccccgg gacatctgtc ggatcagcga ctctaagaag 3180

atgacccaga agcgcgagag caccctgggc aatttctgga tgaagctcct ccagctgcca 3240

gacaagaaga agaagtctac cgaaaagacc aagatcatca agaagatgaa gaaacggatc 3300

atggtccaca tgtgcagctc cctggctctg ccaacaggcc tgacatctct gatcctgcca 3360

agcagcgatc tggccgtgct gatctctagc tccaccagcc actttctgat gcggagccca 3420

gtgctgccta gctccagact gacttgtgcc tctccacagc tgcccagaat gtggacctgg 3480

tccagctggc tgaagtggct gaaaaactgg aagcggttca agcttaaaaa gaagtacatc 3540

ctggccatga cgctgaccct gaacgggcat ccaagaaccg cctctatcac cctgcctacc 3600

accagcctga agggctctag aaccagctgt tgccccagat ctcaccccag actgcctagg 3660

acaaagtggc ctctgcagtg cctgctgcac agcctgggaa caactggaac actcgtgtgg 3720

gaaaggggcg tgctgcagat gagaagtctg gaagctcact gccctctgca gaccttccac 3780

ctccaccact gtagaactca gccagctct tgccctcctg catggattct gtaccaagga 3840

cagtggagcg gagccgtcgt gggacttaga cctgtggctc accatctgcc aagacctatt 3900

cagggcctgc actgcccaca ggctccacat caaccagctc tgtcttgggg caagtggccc 3960

gtgggaatga caggacctgc cagatgttgc cggtggccta gacgggaact caaaaagaat 4020

ctgctgctcc cctgccggtg cagacgcgag tatagagtga caatgggcca agccttcggc 4080

agctttagcg ccgactttag cgctccccag gtgttgaaga agaatcggaa gcagaacaag 4140

attatctgca agggccaccg catcacaagc ctgatcctga gctttcagcg cgtgaaactt 4200

aagaagaaag tcaacgcctg ctctcccgcc tgcacctcta aagcctggac tcccagaaag 4260

ctcgtgggga gagccgtcag aagaaagggc atcatctgta tcaagaatat gttcctgcag 4320

4380

gctctcggcc ctagaatgca gctctgtacc cagctggccc ggttcttccc tatcacacct 4440

cctgtgtggc acatcctggg acctcagagg cacacccctt gtcaaaagaa ggtgccctcc 4500

gctagcccat ggggccttag accatctcct tgcctgagct ggtcctccag aattgtggcc 4560

ccctggacacg ccctgagctg tcggactaga agaagatggt ccagcaaggg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1131

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 1A poly-FSP

<400> 1131

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgaccg agatccagct gaccatgaac 720

gacagcaagc acaagctgga aagccccgct ctgaaacagg tgtcaccagc ctctccacct 780

actcagcagc ctcagacacc tcaggattct agacaggtgc tggccctggc caagaaaacc 840

ctgacaaatg cctccagagc cggacagttt cctgtgtggc ggagaacaag cagaggcatg 900

agcctgcagt ggaagtccgg caatgcctgc acacctctga gatgccctag cagaagccct 960

gccacattcc tgacagccct gaatggctgg cacagatctg gcctgagagc ccagatgcct 1020

agctcttggt ctagcagcgg cagaacactg aagctggccc ctctgcaagt gaccaccact 1080

ctgaagctga tcctggtcat gctggaaatc gtgaccgagc gctccctgct gtactccaga 1140

atgtgtctgg tgcagatccc cacacacacc tgggagcccg attacttcgt gtccctgcct 1200

atgatgttca gctgggccac acagtctttt cccgtgggcg ccgtgtttct gaccagacct 1260

gccattcctt gctgcagctg taccagccac agcaaccctt ggagccagag caatcagcag 1320

ctgaagtgga cccctcacat cctgaagtcc gccagcttcg acaagaaagg cttcgtgatc 1380

ctgagccggc ggagcaagat tgaggcctct cctatcggct ctatcgccag ctctcctgga 1440

catcctctgg gacctacact ggccatcaga ttcatcagcg cccctcagcc tagcagctct 1500

acagtgacag ctgcccctag cggccagtac tttatcgcca agaagcggat caagctgcgg 1560

cggaacaata tgggcggcaa cagatccatc tgcctgacca gaaagcgcaa gagagagaag 1620

cggagccaga agtccaggca gaaccatctg cagctgaaaa gctgcagacg gaagatcagc 1680

aactggtcca agctgagaag cccctggatc ttcaccaagt gcgagatctg cgtgaagaac 1740

accgccatcc tggctatcac cctgatcacc ccctcaggacc ctcatctgga ccctgtgtgt 1800

caccctcata gaaggcctgg atggaagggc caccatagag tggtgttctc tcaccatcct 1860

agcggcagca caaagagcca ctggcagcaa agagaacgcg gaggcggcag acgcgagaag 1920

aagggatgta gagaacagcg gaaggtggtc aagaacccct ctcagcccgt gcctctgaga 1980

aacgatggca tcaccaacgg caacgactcc accagaagct gctccatcag cctgaagaaa 2040

agcctggaaa agatgcccct gatgcggaga gccagaccta ccagctgtag cacctgtaga 2100

2160

atcaccgtgt cttttgtggg acagatcagc accctggtgc tggatgtgtc tagcatcagc 2220

cattggcggc tgccctgtaa agtgaccgag agcagctcca gcttcttcta caccagcgac 2280

aggacccctc tgagcatcag cagaccaacc agcagagcta gacagagctg ttgtctgccc 2340

ggactgacac accctgctca ccaacctctg ggcagcatga gcactagaag agagatgcca 2400

agccaggcca caccttctcc acctggacct tggagagccg ctctgggcaa agaagtgaag 2460

agatcccaga gcaagctgcg cctgagaaac acaagccctg gggagaagaa cgatctccag 2520

ctgttcgtga tgagcgacac cacctacaag atctactgga cagtgatcct gctgaacccc 2580

tgcggcaacc tgcacctgaa aacaacatca ctgcagcggc cacctcggag catcctgtct 2640

ctgaatggaa tctgggaagt gatgccccgg acactggaag gctgcatcag cgtgaaggat 2700

cacagaacca gccagaacca cagcaagtgc atcaccgaat gtgtgcggac tgccctgaac 2760

gtgtccaaga agagactgcc taagctgatg aagaagaagg acagcaccat cttcatcaac 2820

aaagtgcgcc agaacttcaa gcgcaacttc aagaagaaga aacccctcac tttcagcccc 2880

agcagacgga tgactcggag aaccatgaca accaccaaaa tgatgatgat gcggcggacc 2940

aagcggagaa ggcgcaaaag gcggagaatg accatgatga ccagacggac cctgctgaca 3000

aagaaaacct gtctgacccc tagaaccagc gccgccacaa aagctctgcc tagacagacc 3060

atcagccagc ggaggcctat tcagacccca cctggaacat ctctggccat ctggggcaga 3120

acaagactga ccctgacagg caccagatcc agcggaacaa gagcctctcc ttccgccgtg 3180

tgcctgtgtt gttggacctg tccaagcaga agcttcccag ccgcttctcc tagtggactg 3240

agagcacctg ttggagccca ggctcctcct gaagtgacac tgagagcctc ttgtgcctct 3300

agcgcctcta aagcctgtgc tggactttgg ccttggggag cccaagttcg agttggagct 3360

ggaacaggcg gaggacctgc ttgtgttcct gtggctgaag cttctacagg cgcatggaca 3420

gctgaaggcg ccgctggaac aagatgtaga gcctctagag ccccttgccg ccaacttcct 3480

gctagctccc ctaccaagtg tgaaatttgt gtgaagcgca gacggcggag aaagagaagc 3540

agccagtcta gaagccccgt gcctaaaaag cgcgagagga tgatgctgcc tgtgcgggcc 3600

agaacagcta gatctgcact tcctcagggc agagcacctc tggaagggat gctgcttcct 3660

agagcactgc ctagcctgct gagcagaatg cctgccagaa gagagtcccg gttttggctg 3720

gctctgcagg cacttggact cagacctcca catctgccta gctgtcatcc tctgcctcaa 3780

ctgttgggcc ctccacctcc tcatcaggcc ccaatgacac tgaaggccct ggttaagacc 3840

tggacacagt tccgcaacta ccccagagcc gacaagaaga atgtccggtc taccgccttc 3900

ttcctgctgg acatcatcct gggcgattgc ccaagaacaa tggccggacc ttctgcctct 3960

ctgcatcctg catctggcag agcctccaga aagacctggc agagaaggct gagagcttgt 4020

agagcaggcg gacctgctgg atgggctaga caggcctatc ctagactgaa aaccttcggc 4080

gtgcccctgg gctctattct gtgtctggcc ggcagcctgt ccacaatgat gatcattagc 4140

accaccagac aagagtgcgg caggcgggct tctgtgcctt gtagacctat gatcggaagc 4200

gccagaccag gaccttggcg gacatctgct atgcctagtg ccatgggagt cgccctgcct 4260

acctcttgtg aaagcggaag atctcctcct gccaccggcg gaagaatgcc aggatctcag 4320

tctatcatga tgagctggat gcctccactg gctccatgtg ccgcttgtcc atgttctccc 4380

gctcctacac tgtgcccagc acatcctgct agagcaccaa cagccgtgcc tgcctttaca 4440

cctctgtctg ctcatcctgt gccagtgctg tctggatgtc acctggccgt cagaacctcc 4500

atgctgacac tgctgcccat ggaagccaaa aagagcatga agcggtccaa cgctaagaaa 4560

atggaagata tgatctggga gagccggctg ccactgaggc cacaaagagg acctagacct 4620

ctgcacagat ggctgcagca gacaaccggc tgtagccaga ggcggatcgt gttctttctg 4680

gccccatact tccctcggca ccctcacaga tctccaccaa catcttgccc acctctgctg 4740

gaagctttcc cactgccaag agcttctctg agggccacag tgccaagaca tagctctgtt 4800

ctcgccaccg gtctgcacct ccacagacag atttctctgc ccctgagagg attcctcctg 4860

caccaaccac tgccaggaca gcatctgtgg ctgtctcaca gactgaggct gagactgccc 4920

aggacacagc catctttgcc tggaccatgt gcaagcctgc tgtccacact gtctcagcca 4980

ccacctcagg ctccatctca agtgtggact gccgctacac tgagatgtcc tgctgttcct 5040

gcagctgcct gtcctcctgc tctgagatcc atgcctaaga gcgggaacca ggtgctgttc 5100

agacggaatc tgaccccaag ccagctgaga acactggccc caccttatat gctgctgccc 5160

cagtctctgt cccagagcct gagtagaaag cagatcccta gccagtccat gctcgtgggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1132

<211> 5232

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 2A poly-FSP

<400> 1132

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgagaa agatgcagag cctgaatcac 720

cccagcagac tgaccaatgg ctctcctcca ctgcacctgt gccagcctct gagaatgagc 780

agaacccctc ctagaagagt gtgcgccgct cctccacata gactgctggg cccttccaga 840

tggaaccctc ggagaaagac ctggaagatg cggaagaagt acgcccagaa gcctagccag 900

aagaaccacc tgtacggcgg caagaagcgc tctaagttca gcagaagcca cctggacttc 960

agcccctact ggatgaagaa agtgaaggtg ctgttcaagg ccgaccagag cgagtctagc 1020

ctgagcagcg gaagatgcag cctgatggaa cagatccctc acctgaagtc tcggcggaag 1080

tggcagatca gacacgtgct gaagaacctg tggctgccca tgagaagcct gggagaacct 1140

tggagagccg gctttcagtg gggagagtct ccttggcaag tgatggcctc tctgaccggc 1200

agagccacat ttctgctggc ccttgggaa tgtagcctgc ctcaggcaag actgtgcctg 1260

atcgtgtcta gaaccctgct gctggtgcag agccggaaca agaaaacaaa gctgtggttc 1320

agcctgatca acatccacca ccggaagaac ccactgctgc ctatgagatg gtggtgtacc 1380

agaggcggcg gaaagcggaa aaagaaaatg tgcaaggtgt ccggcggctg caccgcctgt 1440

gttgagagat gtccttggaa gggcatctgc tggaagtggc ctgtgacagt ggccgccatt 1500

agaatgtgtg ccctgtctca ccctagcttc tacatcagca gcaccgtgtg ctttctgagc 1560

aagcactgca tgaccgcctg caccagaaag agaatgaaga ccggcgccat ccaagagaag 1620

ggcgctattc aagagaaagg ggccatgacc aagcggatct gcgtggtcaa tttcgaggcc 1680

tgcagagaga tggaaagcct gagcgcccct gagaagatca ccctgtttga agcccacatg 1740

gcccggtgca cctccatcaa tgtgctgtgt gtgcgggcca gcctgattga gaagctgatg 1800

aaagaaaagc gcaagatgaa gcggaaccag gtggcctctc cacagatcat gcagcggctg 1860

ttcatcaagg gctaccagaa caaccacttc accttccatc tgcaaaactt cgccttcttc 1920

agctccttcg gcaacaacaa gtacatcaat atcctgacca tcctgttcca cgtgcggaac 1980

atcgtgtacg gcatccaagt gatgctgtgc gctagccctc agattatgca gagaaccagc 2040

gccgaccggt tccggatgaa gagaagaatg cagaatctgg acaacctggg cgtgccatgg 2100

cctctgttgc ctggaatccc atctctgaac gcccagtgga tgttcctgag aatcgcatcc 2160

ccgcaaatca tgcagaggat gagcgccgtg aacttcgaga caatccggggc cagaaaccgg 2220

aacatgaaga aaaccatgag ccacctgatc gcccatcagg ccctgaagat gagcggcctg 2280

agactgtttc accccagact gctgacacgg aagaagcccg gaaaactgct gctcgcccat 2340

atgtacggct tcagcaccta cggcacccag aaaggcgagt gcgtttgtct gcagtgtctg 2400

agcggcagca gcctgttcat tctgaagcag cccctggacc tgaagatcag caagaccttc 2460

atgggccaca gagaggggcag cgagtctgat aatctgggcc tgctgtatag cttccctcca 2520

ccttatccta tcagcgaggg cctgaccatg accaccggca gaaacatcaa cagcaactcc 2580

ctgatgacca actgcctgtt cagccccatc ttcctgtgcc accacgccat catgcacaga 2640

aagatgacaa gccctcactt caggctgttc agcagcaaga tcccacatcc tcaggtgcca 2700

tctgtggtgg ccctgtgcaa gttccggggcc ttcatccaca cacctagaag ccctggctgc 2760

agcgtgtacg ccaatatgtg ctggcccaag ttcttcctga ccttcagcca gcagtggtcc 2820

agcgtgacat ctctggaatg gacagcccaa gagagaggct gcagcagctg gctgatgaag 2880

cagacatgga tgaagtcttg gagcctgcgg gaccccagct acagatccat cctggaatac 2940

gtgtccacca gagtgctgtg gatgcctacc tctaccgtgc tgcaggctca tccacaagtt 3000

ccagctctgg ctcctcaagc ctggtggcct gctagaagag gacctctgac atctgcccca 3060

tccgctcagc agtccctgac cagagagaag attatcaacc ccaccatcag ctgccccttt 3120

cagagcccca ccaagagact gatctaccct cctagcacct gttctctgcc ctgcagaggc 3180

ttcctgccta gcagatttcc ttggggccca ccctcctctga acagcgtgat cgtgcacaag 3240

atcgtgctgg tgttcctgaa gtccctgtcc agctacaact gcgagcggag aatcagcggc 3300

atccagaaga gagccatcga gttcgccgtg ctgaaactgc tgaatatgga cctgcctctc 3360

gagaacacac agatccccag aacaagccca ctgtggaaga tcacaatggt gctgacatac 3420

cccaactgcg tggtgcatgt gctgatgttt ggcgccctga gagccacagc tcctgctaca 3480

gctgcacctc aggctcagac ccctcattgg agagctgcca caacaagcag catgagcagc 3540

agccctagag ccgagcctac atcttctagc tcttgggcca cctgttggcc cagatgctgc 3600

tccagctctt gttactggtc actgtctagc tggcctccag ctggcgccgc tgaagctaca 3660

aataccagaa ccagaagccg cgagagccag aggggtagaa tgctgacttg gagaagctcc 3720

ctgtggttgc agggcaccag atgttttaca ggcgtgcgga ccagctccag atacaagaaa 3780

agaggcgtga tcgcctggcg gagacagatc aatagacgga agctgcagag aaagaagcac 3840

tggaacagcg cctacctggg cagaacactg ctggtcacct gtatcctgta tcaccggtgg 3900

atcgtgacct ccatgtgtca gtcccagaga tggctgacca gcaccacaag tagatctagc 3960

gccctgatgt ggaccgagct gagcctggaa ccatggattc cctacctgca tcagcagaaa 4020

acccagcgga cactggaatc cgccggactg tttccttggc tgcacaccca gtgccaagag 4080

aagaaaagca gaaagctgat gtccagctgc acacacccct gcacaatgct gctgccacag 4140

ggatgtcatc ctgcctggct gagccctatc tctgcctgtc caagactgct gccatatcct 4200

4260

ttgcaggctc tggctatgtg ggagaaaggc tgggataagc ctggcaccag cctgcagtgc 4320

tacctgaaca gaagaagaaa cagccggaag cagagccagg tgcaactggc cattcaggtg 4380

ctgctcgtgg acaagcacaa gtactttctg ttcggccaaa agaaaggcca catgatcttt 4440

agcctggcca acggcctgtg gtttaacctg attttcaact cccggaagaa actgctcgag 4500

gacatgaagc aaaatggctt caccggcaaa ggcctgacac accccgtgcc tctgaagcgg 4560

actctgcaga acaaaagaaa caagaatatc aaacgcgtca accagaagaa gggcaacatc 4620

aagcggaagt ccatcctgct gcacctgatt gtgctgaata gccccgagag caacctgatc 4680

ctgatccagc atggcggcat cttcaccttt cggttctggg tcgagaagct gtcctggaac 4740

atctgcaaga acctgagcct gtacctggac aagttcgtgc agagactgct gtacgtgtac 4800

4860

atgatggcct tctacctgaa gtacaccgag aaggcctggg tcatggaact ggaatggctg 4920

atccaacagc agtgctatca gggcagctgc ggcagagctt gttcttgtgg aagaggctgt 4980

ggcaccccta gaggcgattg gaagagacat cctcctcggga gagccttctg gaagcctcct 5040

agccagggca tctctagctg tctgatgacc ggctgttggc tcgagaagaa agctacctgg 5100

atgcggcgga cccgccggaa gaagagggaa gatcattgcg gactgagaca ccccagctgg 5160

ccttggacaa gatgtctgag gatgaggcct ccaagaagcg gctaccccta cgatgtccct 5220

gactatgcgt cc 5232

<210> 1133

<211> 5247

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 3A poly-FSP

<400> 1133

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgggag ctgctgctgc ctcttgtcag 720

tctcctcctt ctcctcggga aaccacacct agctccgacg ccaccagacg gacaagacct 780

attgccgctc agagcaagaa aaccatgaca gccgccggac tgacccctat gatccggaag 840

atccagaagc ggcggagaac cgccaaccgg tgtctgagag ttagatgtgg cggctggctg 900

tgtggctgga cacctagagg acagaacacc agaagcacca acctggaaaa gaaccccagc 960

ctgaagaagc agagccagcg gagaggaaag ctgagcagcc tgttcttcag ccactgcctg 1020

tgccggaaca gcagaagggc cacacctaca tctaagggca gcctgcggcg gaagtacctg 1080

agagtgccta ttcctccaca cctgagacac cctctgctgc tgatggacct ggacggaatg 1140

ctgaccgtga tcggacaggg actcgtgggc atgaccccta gagatctggg aacagtggct 1200

ggcgccaccg ctcagattca gaggcctatt caggtgcaga ccggcggcag acttacacct 1260

ccctcctctga ttctgtggcc tcaggccgct cctgttgatc ctcctggctc tgtgtggatt 1320

ctgccctgca agaacaagaa gcggagaaag ctgcggccca agcggctgga ctttcaccct 1380

agatcttcta gcggcgtgtc cgtgatcacc atgaagctgt gggagaagca cttcaagggc 1440

ctgaactgga acctggtcgt ctggattctg aacctgaaga tgatgaacat cgagatcacc 1500

cctcagcgga gcaaggccag aaagtccggc gtgatgaagt gctgggatct gtgccccagc 1560

gagaagaaga ggaagctgcc tctgcctctg accttcagca ccctgctgtg ggactctaga 1620

catagaggcg ccagctccag caccagcaga gactccagaa gctttttcac cagcgctcac 1680

cccagcagac cctgcagaat gagactgtac cagttcctga tccacctgag aaaggtggac 1740

acccaccatc tgcaggtcgt gaccaagacc gtgacagcca caacagctga cggccagaga 1800

atgcggagac tgaagtgcaa ggcctgccgg ctgagatggg ctagacctga acctgctgct 1860

caggcccctc caaagagcca ctttccacct catcctagcg gccctagcag aaccagttct 1920

ctgcagctgc ggagcatccc tcacgtgcca aaagccacag ccgctcacag cgtgacagtg 1980

aagctgcaga agggaaagaa cagcccctgc tgccaaaaga aactgcgggt gcagaatcag 2040

ggccatctgc tgatgatcct gctgcacaat acctacatgg aaatgcacta cggccatcag 2100

gccccacagg tggccatgct gcctaagctg ctgtgcagac agatgctgcc cactctgagc 2160

acactgcagc aaagacaccc acagggccct tgtcctccac ctgtgacacc actgagagtc 2220

cagtctgtcc tgctgctggg agtgcctcag acagctgcta gacagcctag cagacccacc 2280

ggcagaaccc ctaccacaag actgatggcc cctgtcggca gcgtgatgag ctgttctctg 2340

ctgacccagc cttctccagc cagcgcctgt acaatgcctc cactgctgca tcctcagtgg 2400

gccccacatt ctgctggatt gtctcccggc gcttgcagac ctgcctgtac ctgtatcagc 2460

atgaccacca tctccagagc cacctggcag gtttccgtgt acagcggcaa tctgctgagc 2520

ttctccagac ggggcatgaa gagtagcgtg cggctgaaaa acagccggcg gaacagacgg 2580

tacagaaaga tctacggcga gctgaagatg tccctgcagg ccgtggctcc tagatgtcct 2640

agatctgccg tggacatcag ccccggacct acctctccta gcctggcctc ttgtgcctct 2700

gtggccattg gaaaactggc cctgcttggc gcttctgccc cttgtggtgc tgctggcgct 2760

agagaggctg gaagatttgc cgtgctgggc accatggtca tgaagcacct gaaccgcaag 2820

ctgcatctga cccggtcctg cctgatcgat ctgaccatga tgatgagcca gaagctctgg 2880

aagaaccagc ggaagagaat ccctctgcca tctcctagac agcatctgct ccctcagtac 2940

cctctgcacc ctcctccacc ttgtctgctg ctgctccacc acagacacca cctggccttt 3000

ctggaaatgg cctgcagcag ccagcacacc cactccatca agatctggat cagctttagc 3060

cacgagcaga gcagcccatt cctggccaga tcctggaaca ccatgcagtg gaacaacaag 3120

aaaatggcca tgacagtgat cgctggcgcc caccactgtc agtacaagct gacctacacc 3180

aaagtgtgga agatgaattg gaagaaaatg atgatcggct cccagcacct cctgttcagc 3240

cctctggata gctctccact gcaactgctg cttcaccctc acggcaagag atgcaaccct 3300

tgctgcagac tgacatggat gtccatgctg cagaaacagt gctgctacaa cctcgtgatc 3360

aagcaccac tgatcttcag aatcaacctg cggagacagg gcaagaccaa ggatggcgtt 3420

gtgcagagcc tgggcttcct gaaccagcag atcatcctgc agaaagagtt cttcatctgc 3480

ctgctcatgt ttatcaagcg gtggaagcct gccgccacca agagagagtg ggagaaacgc 3540

aagcagatcc tcggcgggagg cggcaagtac aaagagtact tcctgaagcg catcctgatt 3600

cggaaggcca tgactgtgct ggccggcgac aagaaaggcc tgggcagatt catgagatgc 3660

gtgcagagcg agacaaaggc cgttagcctg cagctcccac tcggaagagg ccctgtgtac 3720

tacgtgtgca accggatgaa cgtgtccagc gtgctgaagc ccttcctgtt caccgtgcct 3780

atcgaggaca tgagagagtg cacactgggc cgcaacagaa tgaatgtgtc ctctgtgctg 3840

aaaccctgtc tgatccctgt gccaatcgac ctgcctcgga tgctgccaat cagcagccca 3900

ctgcacagac cccagatgcc attctggacc tgcacctttc ctgccacaac atggggcacc 3960

tgggagacac cttctacatg gggctggcgg acctttcggg gccctagaag aaaagtgctg 4020

atggtgctgt ggctgcccgc caaccagagt ctgcagaaaa gccaagtgtt ccagtgggtc 4080

ctgcggaccg agaacatccg gaagctgatc tggaccaagc agcggagctt cctgtctctg 4140

ttccccaagc tgagagccac cgaacagatg accaatgtgg gctgcgcctt cagcggccgg 4200

attctgcatc aacctgaaat ggccgccatg cggctgatgc ccaagttcct gaatagcacc 4260

aacagctggt ggacctgtac caacctgagc gtgcccatga tgctgaccat tctgatttgg 4320

aagcgggtgt tcattctcct gctgtccgac aagaagctgc tgaagaagtc tagccagctg 4380

aggcggtctt ggagaaacgt gtcccggatc agccaccaga tgaagtgtct gaccgccgtg 4440

gactgtctgc aggccttctg ggacatgaac atggaacacc ggctgaccat ggacagcgcc 4500

ctgagcctga caaagagaga gggacccgag acttgcaaga agaagctcct gctgaaactg 4560

ctgacaatga gcctgagcaa catcctgatg aagttccggc tgcggctgag aatcccaagc 4620

ctgaggaagt atcgccagca tctgaaaaag accagcgcca agggcgagaa gctgaagcac 4680

tgctttaagg cttggggcag aggcccacct ctgcacacaa gaagccagaa gaaagaccct 4740

gccggcaagc tgaaaaaact gaccacactg agcttcgtgc tgatcgagga acggcggaac 4800

ctgcagcgga atctgaaagt cctgtggctc aagaaaagca ccaagagccg gtatctgttc 4860

ttcctgaccg tgtgcggctt cttcgagaag aaacggcctg agtggctgtc ctccagaaag 4920

aagggacaca caaagaatcg gaggaaggcc aagcggaagc cccagatcgt gcacaagaag 4980

agaggcctgt tcctcaaaaa gaaatacctg gtgcagggcg ccaagaagat caccttcctg 5040

tccaacggca tggaagcttg cggcacacac cccacaccaa gactgagcga cagagtgttc 5100

ctggtcaaga tcaccaccac cacaagcagc cccagaagaa gcccagcact gagagctaga 5160

ggcggcacca caattggcga agtgaccagc ctgtgcttct tctacctgct gtacggctac 5220

ccctacgatg tccctgacta tgcgtcc 5247

<210> 1134

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 4A poly-FSP

<400> 1134

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgatcc agaagaagaa acagaaatcc 720

atggccaaga agtcccagca gcctctggtg ctgaagaaga accggaagca agagtgggga 780

ggacaggccc acagacagag aaacaacgcc aagaagatgc ccttccaaga ggaaaagaag 840

aacggccgga tcagcagcag cctgatcctg agcttccaga gagtgaagga cagcaagaaa 900

atgacccaga agatgtgcag ctctctggcc ctgcctacag gcctgacatc tctgatcctg 960

cctagcagcg atctggccgt gctgatctct agcagcacca gccactttct gatgcggagc 1020

cctgtgctgc caagctccag actgacatgt gcctctccac agctgccccg gatgtggaca 1080

tggtctagct ggctgaagca ccccagaacc gctagcatca cactgcctac cacaagcctg 1140

aagggcagca gaaccagctg ctgcccaaga tctcacccca gactgcctag gaccaagtgg 1200

cctctgcagt gtctgctgca cagcctggga acaacaggca cactcgtttg ggaaaaggggc 1260

gtgctgcaga tgagaagcct cgaagcccat tgtcctctgc agaccttcca tctgcaccac 1320

tgcagaaccc agccaagctc ttgtcctcct gcctggattc tgtaccaagg ccagtggagc 1380

ggagccgtcg ttggacttag acccgtggct caccatctgc ctagacctat ccagggcctg 1440

cactgtcctc aggctcctca tcaacctgct ctgagctggg gaaagtggcc cgtgggaatg 1500

acaggacctg ccagatgctg tcggtggccc cgcagatctc agaagtctca gcatcactgc 1560

gccctgcgga agatcgagtg ggccacaaaa ctgtctctcg gcggaaaagc cggaccacag 1620

gctcaagaga aggccagacc ttctccttgc ctgtcctggt ccagcagaat tgtggcccct 1680

ggacacgccc tgagctgtcg gactcgtaga agatggtcct ccaagtgcag aggcggccct 1740

agcgccagcg atagatgttg tagctgtagc cccagcggct tcagcgccgg aagaggcaga 1800

tgtcctgtgc agggatgtct gaggccacac agagttcagc tgctgagaag atggggccca 1860

ggatctcctg ctggccagag actgtctaag ggcttccaac tgctgcgttg gtggggacct 1920

ggatctccag ctcctgagcc tagaaagggc ccatttcctc cacctgatcc tccatggcct 1980

gtgaccgctg tgacagtgat ggctggctct gtgcctagcg cacagtctgt ggatgccctg 2040

gaatctcctg gacctctggc tctggaaggc cctagcagcc ctagaaacct gctgtggcgg 2100

gaaatgagca tctttctgcc cggcatcttc tgccaaaaga aggtgccatc tgccagcccc 2160

tggggacttg ttcctccttc tccaccactc gctctgggcc ctagaatgca gctgtgtacc 2220

cagctcgcca gattcttccc catcacacct ccagtgtggc acatcctggg acctcagaga 2280

cacacacctc aggtggtcct gagactgggc aacctgcctc cattcttcag aggcagcgag 2340

gtgccctttc ctagaccttg ccggaccaag aacgagggca acaatcactg gccgagtg 2400

gaaatgccta ccggctggct gctcgtgggc tacaacacca gaaacacagc ccagaatcct 2460

cggctgcagc agcatctgaa acactggcag cagtgcacac gggccagatg gtgtcaagag 2520

ccttgtcaga ccctgacctt cttccacttc ttcttctttt tcaaaacggt gtccctgtgt 2580

tgtcccggct ggtccaaagg acaacctcca cctcctggcc tgaagccttc tagcctggct 2640

tctcaggtgg ccagaaccac aggcgtgtac cagcacgtgt ggctgatctg cttcgtgttc 2700

ttcgtgtgcg tggaaaccgg cttctgtcac gtggcacaag tgggcctcga gagactggtt 2760

tctctgagcg gcatcaccga cgtgaaccat tgggcccagc ctaagccatc tcctagcaaa 2820

ggcaccggca tgaacagacc catcggcctg agagttggca aaggcgtgtg ggccagactg 2880

ctcaagaaga agcctagaga catctgtcgg atctccaaca gaaagacccg gaaggccgcc 2940

agagagaagc accgcaaaac agatagagcc agcgtgtcct tctggactct gctgcctaca 3000

acaggcgtgc ggacaagagg aagccctctg agactgactc tgtggcctcc tagatgcaga 3060

3120

atcgccgtgc tgcaagaaac cgccaagaaa agccctacca gcttctgctg caacctggac 3180

ctgcagcatc agcgcagacg caaaaagaag aaagacaagg tcgagatccg gaatcggagc 3240

cccaaggcca atagcacaga gtgtcagggc gagcacagca gagagcagaa tctgctgaat 3300

3360

agacaccacc accacaatca aaagaagatg agcgtcccac tgttcttcca cggcgacctg 3420

tgtagagaga gcaccctggg caatttctgg atgaagctgc tccagctgcc tcagaggcct 3480

ccactgcaac cagtgaccgg aatggaaatc ctgctttgga ttctgctgaa gatggaaatt 3540

cagatcttca agatttgcct gggcgagatg cagctctgca cctctcggat tctgaacctg 3600

caagtgctga aaaagatcct gcggagcttt atgaagctgt accacagcct cgtgatgtgc 3660

ctgcggctga ggaccaagct ggaaaaggct ctgagcgccc tgttcatctg gcctcagcac 3720

tcctacaaga tcacacaccc attcgccagc cagccactgg gacaagagat gcccaacaac 3780

accgatcc ggctgctgaa gcccgacaag aagaagaaaa gcaccgaaaa gaccaaaggc 3840

caggctttcg gcagcttctc cgccgatttt tcagcccctc aaggacaggc ttttggctcc 3900

ctgtgtcagc tggtgtccgc cgactttagc gccccacaag tgaccagact gcggagcctg 3960

aaccatctgc tgatttggct gaagaattgg aagcggttca agctcaaaaa gaaatatcag 4020

gcccctctgc ggctgtggtc ttggtgcgga acaagccaga cctacttcgg cagcatcatc 4080

tgtatcaaga atatgttcct gcagcggaag ggtttcctga acctgagcca cgtggaaaag 4140

ttcgagctta agaagaatct tctgctcccc gtcgtgatca acctgaagag actgcagaaa 4200

ttcatgctga accagaagtg cctggctcca ttttcctgcct gcaccagcaa agcctggaca 4260

cccagaaaac tcgtgggcag agccgtgcgg agaaagggca ttctggccat gacactgacc 4320

ctgaacggaa tcgaggccct gacagccctg tttacctgta gaaccgcctc catcagcgtg 4380

aacatcagca ccctgatgag catcctggaa gccatcatca agaagatgaa gaaacggatc 4440

atggtgcacc ggtggatctt ctccaatcag ctgaccgtgc agatgctctg cctgcctctg 4500

ctgacccctg acaccagatg gatctttagt aatcagctgc agacagtgca aatgctgtgt 4560

ctgccacctc tgaagcaggg cgagagaatc atttggccac tcggcggctt tcagatcccc 4620

aatttcttct ggtccctcgg catcggacac actggcgcat ggtggcatac tggtagaagg 4680

cggagagagt ggggcggctc taccgtgtct agaagcccac tgtggatcag ctccatgatc 4740

tacctgagag gcatggcctt cgccagcgac agctccatca gaaccaccag agtgtcctgg 4800

tgtcggagat gtggcgccaa gtctgccgca gcctcttctt ctccactgtg ggattgcagc 4860

accacaaccg gcacacacca cggatgcctg tgggagatcc ctagcgttac cctgctggct 4920

aacctgcacc tgtgctacta ccactctcag agcaacagaa acaagagccg ccagatggaa 4980

tccctgggca tgaagctcca acttaaaaag aaagtgaacg cctgcagcac ctacacaccc 5040

cacatctgca gacacacctg tcgcgtgaac gccgtcgagg aactgcactc tagaaggatg 5100

tccgtgcgga tcgtgaaaaa ttgggtgtcc tgctgctacg tgcggctgtc agctgtggga 5160

ctgtctactt ggagcgctct ggacaacaag atcatctgca agggccacag aatcaccggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1135

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 1B poly-FSP

<400> 1135

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgaccg agatccaatt gaccatgaac 720

gacagcaagc acaagctgga aagccccgct ctgaaacagg tgtcaccagc ctctccacct 780

actcagcagc ctcagacacc tcaggatagc agacaggtgc tggccatgcc tagcagctgg 840

tctagctctg gcagaaccca gcggaaggtg gtcaagaatc ctagccagcc tgtgcctctg 900

agaaacgacg gcatcaccaa cggcaacgac tccaccagaa gctgcagcat cagcctgaag 960

aaaagcctgg aaaagatgcc cctcatgatg aagaagaagg acagcaccat cttcatcgtg 1020

cccatgagcc tggcctgtag catcaccgtg tctttcgtgg gccagatcag cacactggtg 1080

ctggatgtgt ccagcatctc ccactggaac aaagtgcggc agaacttcaa gcggaacgcc 1140

gacaagaaga acgttcggag cggcgagaag aacgacctgc agctgtttgt gatgagcgac 1200

accacctaca agatctactg gaccgtgatc ctgctgaacc cctgcggcaa tctgcacctg 1260

aaaaccacca gcctgggcga ctgtcctaga acaatggccg gaccttctgc ctctctgcac 1320

cctgcatctg gcagagcctc tagaaagacc tggcagagaa ggctgagagc ctgtagagct 1380

ggtggacctg ctggatgggc cagacaggcc ttcgataaga aaggctttgt gatcctgagc 1440

gtcggagccg gaacaggcgg aggacctgct tgtgttcctg tggctgaagc ttctacaggc 1500

1560

cagctgcctg ccagctctcc taccaagtgc gagatctgcg tgaagcgcag acggcggaga 1620

aagagaagca gccagtctag aagccccgtg cctaagaaac gcgagagaat gatgctgccc 1680

gtgcggggcca gaacagctag atctgcactt cctcaggggga gagcccctct tgagggaatg 1740

ctgcttccta gagcactgcc cagcctgctg tctagaatgc cagccagaag agagagccgg 1800

ttttggctgg ctctgcaggc acttggactc agacctccac atctgcctag ctgtcatcct 1860

ctgcctcaac tgcttggccc tccacctcct catcaggccc ctacagcttt cttcctgctg 1920

gacatcattc tgcggctgcc ctgcaaagtg accgagtcta gcaatatggg cggcaacaga 1980

tccatctgcc tgaccaacgc caagaaaatg gaagatatga tctggggagag cacgcccctg 2040

agcatcagca gacctacatc tagagccagg cagagctgtt gtctgcccgg actgacacat 2100

cctgctcacc agccactggg atctatgacc ctggccatca gattcatcag cgctcctcag 2160

cctagctcca gcacagtgac agctgctcct agcggccagt acttcaaggg caagaagcgg 2220

cggaaagaga tcttcaagaa gaagaaaccc ctgacattca gccccagcct ggccaaaaag 2280

accctgacca atagcagctt cttctacacc agcgaccgcg aggccaagaa atctatgaag 2340

cggagcatga ccctgaaggc cctggtcaag acctggacac agttcagaaa ctaccccaga 2400

gcctcctctc ctggacaccc tctgggacct aatggcatct gggaagtgat gccagaaca 2460

ctggaaggct gcatctccgt gaaggaccac agaacaagcc agaaccacag caagtgcatc 2520

accgagtgtg tgcggacagc cctgaacaga agaagcaaga tcgaggcctc tcctatcggc 2580

tctatcgccc tgagatccat gcctaagagc agaaaccagg tgctgttcag acggaatctg 2640

accccaagcc agctgagaac tctggcccct ccttatatgc tgctgcccca gtctctgagc 2700

cagagcctga gcagaaagca gatccctagc cagtccatgc tggtggcctc tagagcagga 2760

cagtttcctg tgtggcggag aaccagcaga ggaatgagcc tgcagtggaa gtccggcaat 2820

gcctgcacac ctctgagatg ccctagcaga agccctgcca cattcctgac agctctgaac 2880

ggctggcaca gatctggact gagagcccag agaaagcgca agcgcgagaa gagaagccag 2940

aagtccaggc agaaccatct gcagctgaag tcctgccggc gcaagatctc caactggtcc 3000

aagctgagaa gcccctggat cttcgtgtcc aagaagagac tgcccaagct gaccaagtgt 3060

gaaatctgtg tcaagaacac cgccatcctg gctatcaccc tgatcacccc tcaggaccct 3120

catctggacc ctgtgtgtca ccctcataga aggcctggat ggaagggcca ccacagagtg 3180

gtgttttctc atcaccctag cggcagcacc aagagccact ggcagcaaag agaacgcgga 3240

ggcggcagac gggaaaagaa gggctgtaga gagagatccc tgctgtactc cagaatgtgc 3300

ctggtgcaga tccccacaca cacctgggag cccgattact tcgtgtctct gcccatgatg 3360

ttcagctggg ccacacagtc attccccgtg ggagccgtgt ttctgaccag acctgccatt 3420

3480

agcccaccta cctcttgtcc tccgctgctg gaagcctttc cactgcctag agcatccctg 3540

agagccaccg tgccaagaca tagctctgtg ctggcaaccg gactgcatct gcacagacag 3600

atttctctgc ccctgagagg attcctgctg catcaaccac tgccaggcca gcatctgtgg 3660

ctgtctcata gactgcggct gagactgcca agaactcagc ggcctcctag atccatcctg 3720

agccttagac tgccactgag gccacagaga ggacccagac ctctgcatag atggctgcag 3780

cagaccacag gctgtagcca gagaagatac cccagactga aaaccttcgg cgtgccactg 3840

ggctccattc tgtgtctggc tggatctctg agcaccatga tgatcattag caccaccaga 3900

caagagtgtg gcaggcgggc atctgtgccc tgtagaccta tgatcggaag cgccagacca 3960

ggaccttgga gaacatctgc tatgcctagc gccatgggcg tcgcactgcc tacaagctgt 4020

gaatccggaa gatctcctcc tgccaccggc ggaagaatgc ctggaagcca gagcattatg 4080

atgagctgga tgcctcctct ggctccttgt gccgcttgtc cttgttctcc cgctcctaca 4140

ctgtgtcccg ctcatcctgc tagagcacct acagccgtgc ctgcctttac acctctgtct 4200

gcacatcctg tgcctgtgct gtctggatgt cacctggccg tcagaacctc catgctgaca 4260

ctgctgccta tgctgaagct ggccccactg caagtgacca ccacactgaa gctgatcctg 4320

gtcatgctgg aaatcgtgac cgagagcact cggagagaga tgccttctca ggccacacct 4380

tctccacctg gaccttggcg agccgctctg ggcaaagaag tgaagagatc ccagagcaag 4440

ctgcgcctga gaaacacaag ccccatcgtg ttctttctgg ccccatactt ccccagacgg 4500

atgaccagac ggaccatgac aaccaccaaa atgatgatga tgcggcggac caagcggaga 4560

aggcgcaaaa ggcggagaat gacaatgatg acaaggcgga ccctgctgac aaagaaaacc 4620

tgtctgaccc ctagaaccag cgccgctacc aaagctctgc caaggcagac aatctcccag 4680

cggaggccta ttcagacccc tcctggaaca tctctggcca tctggggcaa ccagcagctg 4740

aaatggaccc ctcacatcct gaagtccgcc agcagaaggg ccagacctac cagctgtagc 4800

acctgtagaa tgtggtggat cgccaaaaag cggatcaagc tgcggcggaa cagaaccaga 4860

ctgacactga caggcacaag aagcagcggc accagagctt ctccatccgc cgtttgcctg 4920

tgttgctgga cctgtccttc cagaagcttc ccagccgctt ctccaagcgg attgagagca 4980

cctgttggag cacaggcccc tccagaagtt acactgagag ctagctgtgc ctccagcgcc 5040

tctaaggctt gtgctggact ttggccttgg ggagcacaag ttcggcaacc atctttgcct 5100

ggaccatgcg cttctctgct gagcacactg tctcagccac ctccacaggc tccatctcaa 5160

gtgtggacag ccgcaacact gagatgtcct gctgttccag ctgctgcctg tcctcctggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1136

<211> 5232

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 2B poly-FSP

<400> 1136

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgagaa agatgcagag cctgaatcac 720

cccagcagac tgaccaacat gagcagaaca cctccaagac gcgtgtgcaa ccctcggaga 780

aagacctgga agatgcggaa gaagtacgcc cagaagccta gccagaagaa ccacctgtac 840

aacagccgga agaaactgct cgaggacatg aagcagaatg gcttcacagg ccacagagag 900

ggcagcgagt ctgataatct gggcctgctg tactcctttc ctcctatccg gatgtgcgcc 960

ctgtctcacc ctagcttcta catcagcagc accgtgtgct ttctgagcaa gcactgcatg 1020

accgcctgca cacaaaagaa aggccacatg atcttcagcc gcgagaagat catcaacccc 1080

accatcagct gcccctttca gagccccaca aagcggctgg aactgagcct ggaaccttgg 1140

attccctacc tgcaccagca gaaaacccaa ggcggcaaga agagaagcaa gttccctggc 1200

accagcctgc agtgctacct gaacagatgc agcctgatgg aacagatccc tcacctgaag 1260

tccgcctctc cacagatcat gcagagaacc agcgccgacc ggttccggat gaagcggaga 1320

atgcagaacc tggacaatct gggcgttcca tggccactgc tgcctggacc tctggaaaac 1380

acacagatcc ccagaacaag ccctctgtgg aagatcacaa tggtgctgac ataccccaac 1440

tgcgtggtgc acgtgctgat gttctggctg cctatgagat ctctgggcga gccttggaga 1500

gctggctttc agtggggaga gtccccttgg caagtgatgg ctagcctgac aggcccctat 1560

cctatcagcg agggcctgac catgaccacc ggcagaaaca tcaacagcaa ctccctgatg 1620

accaactgcc tgttcagccc catcttcctg tgccaccacg ccatcatgca ccggaagatg 1680

acaagcccac acttcagact gttcagcagc aagatcccac atcctcaggt gccatctgtg 1740

gtggccctgt gcaagttcag agccacattt ctgctcgccc tgtgggagtg tagtctgcct 1800

caagccagac tgtgcctgat cgtgtctaga accctgctgc tggttcagtc tgccgctcct 1860

ccacatagac tgctgggccc ttctagatgg tccagaagcc acctggactt cagcccctac 1920

tggatgaaga aagtgaagct ggccaacggc ctgtggttca acctgatctt cgtgctgttc 1980

aaggccgacc agagcgagtc tagcctgtct agcggccgga acaagaaaac aaagctgtgg 2040

ttctccctca tcaacatcca ccaccgcaag aatcctctgc tgcccatgag aatccccagc 2100

ctgaatgccc agtggatgtt cctgagaatc ctgcttctgg cccatatgta cggcttcagc 2160

acctacggca cccagaaagg cgagtgcgtt tgtctgcagt gtctgagcgg cagcagcctg 2220

ttcattctga agcagcccct ggacctgaag atctccaaga ccttcatgct gcaagctcac 2280

ccacaggtgc cagctcttgc tcctcaagct tggtggcctg ctagaagagg ccctctgaca 2340

tctgctccat ccgctcagca gtctctgaca gctagccctc agattatgca gcggatgagc 2400

gccgtgaact tcgagacaat catctaccct ccaagcacct gtagcctgcc ttgtagaggc 2460

agaacactgg aaagcgccgg actgtttcct tggctgcaca cccagtgtac acacccctgt 2520

accatgctgc tgccacaagg ctggtggtgt acaagaggcg gcggaaagcg gaaaaagaaa 2580

atgtgcaagg tgtccggcgg ctgcaccgcc tgtgttgaga gatgtccttg gaagggcatc 2640

tgctggaagt ggccagtgac agtggctgct cagtggtcta gcgtgacctc tctggaatgg 2700

acagcccaag agagaggctg cagcagctgg ctgatgaagc aaacatggat gaagtcttgg 2760

tccctgcggg accccagcta cagatccatc ctggaatacg tgtccaccag agtgctgtgg 2820

atgcccacaa gcaccgtgcg gagaagaaac agcagaaagc agtctcaggt gcagctggcc 2880

attcaggtgc tgctcgtgga caagcacaag tactttctgt tcggcagagc ccgcaaccgg 2940

aacatgaaga aaaccatgag ccacctgatc gcccatcagg ccctgaagat gagcggcctg 3000

aggctgtttc atcctcggct gctgacaaga aagaagcccg gcaagttcct gcctagcaga 3060

tttccttggg gccctcctcc tctgtgtcat cctgcttggc tgagccctat ctccgcctgt 3120

ccaagactgc tgccatatcc ttttcacgtg ctggccctgg aagtgacctt tccacctgga 3180

acagccatgc ctcagatgca actgcaggcc ctggccatgt gggagaaagg ctgggataag 3240

cggaagagaa tgaagaccgg cgccatccaa gagaagggcg ctattcaaga gaaaggggcc 3300

atgaccaagc ggatctgcgt ggtcaatttc gaggcctgca gagagatgga aagcctgagc 3360

gcccctgaga agattaccct gtttgaagcc catatggccc ggtgcacctc catcaatgtg 3420

ctgtgtgtca gagcctctct gatcgagaag ctgatgaaag aaaagcgcaa gatgaagcgc 3480

aaccaggtgg catccccaca aattatgcag aggctgttca ttaagggcta ccagaacaac 3540

cacttcacct tccatctgca aaacttcgcc ttcttcagca gcttcggcaa caacaagtac 3600

atcaatatcc tgacgcggcg caagaaaaga gaggaccact gcggactgct gatcctgatc 3660

cagcacggcg gcatcttcac ctttcggttc tgggtcgaaa agctgagctg gaacatctgc 3720

aagaacctga gcctgtacct ggataagttc gtgcagcggc tgctgtacgt gtacatcatc 3780

gagacaaacg tgttcgtgct gaacctgttc cacgtgccag tgaaccagat cttcatgatg 3840

gccttctacc tgaagtacac cgagaaggcc tgggtcatgg aactggaatg gctgatccaa 3900

cagcagtgtt accagcaaga gaagaaaagc cggaaactga tgagcagctg cggggccttc 3960

atccacacac ctagaagccc tggctgcagc gtgtacgcca atatgtgctg ggccttttgg 4020

aagcctccta gccagggcat cagctcttgt ctgatgacag gctgttgggg cagccctcct 4080

ctgcacctgt gtcagcctct gaggcggtat aagaaaaggg gcgtgatcgc ctggcggcgg 4140

agaaagtggc agatccggca tgtgctgaaa aacctgatcc tgtttcatgt gcggaacatc 4200

gtgtacggca tccaagtgat gctgtgcggc aagggactga cacaccctgt gcctctgaaa 4260

cggaccctgc agaacaagag gaacaagaac atcaagcggg tcaaccagaa gaagggcaac 4320

atcaagagga agtccatcct gctgcacctc attgtgctga atagccccga gagcaacccc 4380

aagttcttcc tgacctttag ccagctcgag aagaaagcta cctggatgag aagaacaggc 4440

gccctgagag ccacagctcc tgctacagca gctcctcagg ctcagactcc tcattggaga 4500

gccgccacaa caagcagcat gagcagcagc cctagagccg agcctacatc ttctagctct 4560

tgggccacct gttggcccag atgctgtagc tccagctgtt actggtccct gagttcttgg 4620

ccctcctgctg gcgccgctga ggctacaaac accagaacta gaagcagaga gtcccagcgg 4680

ggcagaatgc tgacttggag aagtagtctg tggctgcagg gcaccagatg ttttacaggc 4740

gtgcggacaa gcagcggaag ctgtggcaga gcttgtagct gcggaagagg ctgtggaacc 4800

cctagaggcg attggaagag acaccctcct cggaggaata gcgtgatcgt gcacaagatc 4860

gtgctggtgt ttctgaagtc cctgtccagc tacaactgcg agagaaggat cagcggcatc 4920

cagaagagag ccatcgagtt tgccgtgctg aaactgctga atatggacct gcactggaac 4980

tccgcctacc tgggcagaac tctgctggtc acctgtatcc tgtatcaccg gtggatcgtg 5040

acctccatgt gccagtctca gagatggctg accagcacca caagcagatc cagcgctctg 5100

atgtggaccc ggcagatcaa tcggaggaag ctgcagagaa agaagaggca cccaagctgg 5160

ccttggacca gatgccttag aatgaggcct cctagaagcg gctaccccta cgatgtccct 5220

gactatgcgt cc 5232

<210> 1137

<211> 5247

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 3B poly-FSP

<400> 1137

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgggag ctgctgctgc ctcttgtcag 720

tctcctcctt ctcctcggga aaccacacct agctccgacg ccaccagacg gacaagacca 780

attgccgctc agagcaagaa aaccatgaca gccgccggac tgacccctaa gaacaagaag 840

cggagaaagc tgcggcccaa gatgatccgg aagatccaga agagaaggcg gaccgccaac 900

cggcggaata gcagaagggc tacccctaca agcaagggca gcctgagaag aaagtacctg 960

agagtgcggg gacctcctct gcacacccgg aacatcagaa agctgatctg gaccaagcag 1020

cggagcttcc tgtctctgtt ccccaagctg agagccaccg agcagatgac caatgtggggc 1080

tgctctatcc ctcacgtgcc caaagccaca gccgctcact ctgtgacagt gaaactgcag 1140

ctgcccctga ccttcagcac cctgctgtgg gatagcagac acaccatgag cctgagcaac 1200

atcctgatga agttccggct gcagtctagc ccattcctgg ccagatcctg gaacaccatg 1260

cagtggaaca acaagaaaat ggccatgacc gtgatcgctg gcgcccacca ctgtcagtac 1320

aagctgacct acaccaaagt gtggaagatg aactggaaga aaatgatgat cggcagccag 1380

catctgctgt tcagccctct ggatagcagt cccctgcagc tcctgctgca ccctcacggc 1440

aagagatgca acccttgctg cagactgacc tggatgagcc agaacaccag aagcaccaac 1500

1560

agcggcagaa tcctgcacca gccagaaatg gccgccatga gactgatgcc caagttcctg 1620

aactccacca acagctggtg gaccctgacc acactgagct tcgtgctgat cgaggaagcc 1680

agacagccca gcagacctac cggcagaaca cctaccacca gactgatggc ccctgtgggc 1740

agcgtgatga gctgttctct gctgacccag ccttctccag ccagcgcctg tacaatgcct 1800

ccactgctgc atccacagtg ggcccctcat agtgctggac tttctccagg cgcttgcaga 1860

cctgcctgca cctgtatcag catgaccacc atcagcaggg ccacatgggg cccagtgtac 1920

tacgtgtgca accggatgaa cgtgtccagc gtgctgaagc ccttcctgtt caccgtgcct 1980

atcgaggaca tgagagagtg caccctgggc agaaacagaa tgaatgtgtc ctctgtgctg 2040

aaaccctgtc tgatccctgt gccaatcagc gagaagaaga ggaagctgcc caacatcgag 2100

atcacccctc agcggtctaa ggccatgctg cagaagcagt gctgctacaa cctcgtgatc 2160

aagcaccctc tgatcttcag aatcaacctg cggcggcagg gcaagaccaa agatggcgtt 2220

gtgcagagcc tgggcttcct caaccagcag atcatcctgc agaaagagtt cttcatctgc 2280

ctgctcatgt tcatcaagcg gtggaagcct gccgccacca agagcagaaa gaagggccac 2340

accaagaaca gacggaaggc caagagaaag ccccagatct acctgttctt cctgaccgtg 2400

tgcggctttc acctgaaaaa gaccagcgcc aagtggctga agaagtccac caagtccaga 2460

2520

agatgcggcg gctggctgtg tggctggaca cctagaggta gaaagtccgg cgtgatgaag 2580

tgctgggatc tgtgccccgt gcacaagaag agaggcctgt tcagactgga ttttcacccc 2640

agaagcagct ccggcaaggg aaagaacagc ccctgctgcc aaaagaaact gcgggtgcag 2700

aaccagggcc atctgctgat gattctgctg cacaatgacc gggtgttcct cgtgaagatc 2760

ggcgagaagc tgaagcactg tttcaaggcc tggggctcca gacggaaccg gcggtacaga 2820

aagatctacg gcgagctgaa gatgtccctg caggccttcc tgatccacct gagaaaggtg 2880

gacacccacc acctccaggt ggtcaccaaa acagtgaccg ccacaacagc cgacggccag 2940

agaatgcgga gactgaagtg caaggcctgc cggctgagat gggccagacc tgaacctgct 3000

gctcaggccc ctcctaagag ccactttcca cctcatccta gcggccccag cagaacaagt 3060

agcctgcagc tgagaagcct gttctttagc cactgcctgt gcagccagaa gaaggaccct 3120

gccggcaagc tgaaaaagct gctgaagaaa agcagccagc tgcggagaag ctggcgcaat 3180

gtgtccagaa tcagcacccc tcctccactg atcctgtggc ctcaagctgc tcctgtgtcc 3240

gtgatcacca tgaagctgtg ggagaagcac ttcaagggcc tgaattggaa cctggtcgtg 3300

tggattctga acctgaagat gatgacctac atggaaatgc actacggcca tcaggcccca 3360

caggtggcaa tgctgcctaa gctgctgtgc agacagatgc tgcccactct gagcacactg 3420

cagcagagac atcctcaggg cccttgtcct cctcctgtga cacctctgag agtccagtct 3480

gtcctgctgc tgggagtgcc tcagacagcc agaggaccta gaagaaaggt cctgatggtg 3540

ctgtggctgc ccgccaatca gtctctgcag aaaagccagg ttttccagtg ggtgctgcgg 3600

acagaggtgg cccctagatg tcctagatct gccgtggaca tcagccccgg acctacatct 3660

cctagcctgg cctcttgtgc ctctgtggcc attggaaagc tggcactgct tggcgcctct 3720

gctccttgtg gtgctgctgg cgctagagag gccggaagat ttgctgtgct gggcaccatg 3780

3840

tacagacagc acctgaaccg gaagctgcac ctgacaagat cctgcctgat cgatctgacc 3900

atgatgatgt cccagaagct ctggaagaac cagcggaaga gaatccctct gccatctcca 3960

cggcaacatc tgctgccaca gtaccctctg cacccacctc caccttgtct gctgttgctg 4020

caccatagac accacctggc ctttctcgag atggcctgta gctctcagca cacccacagc 4080

atcaagatct ggatcagctt cagccatgaa gtggaccctc caggcagcgt gtggatactg 4140

ccttgcgatc tgcctcggat gctgccaatc tcttcacccc tgcacagacc ccagatgcct 4200

ttctggacct gcacctttcc tgccacaacc tggggcacat gggagacacc ttctacatgg 4260

ggctggcgga ctttcagagg cgctagcagc agcacatccc gggacagcag atccttcttc 4320

acctctgctc accctagcag gccctgcaga atgagactgt accagtgcaa gaagaagctc 4380

ctcctgaaac tgctgaccac caccacaagc agccccagaa gatcccctgc tctgagagca 4440

agaggcggca ccacaatcgg cgaagtgacc agctgtacca acctgagcgt gcccatgatg 4500

ctgaccatcc tgatttggaa gcgggtgttc atcctcctgc tgagcgataa gaagggcgcc 4560

aagaagatca ccttcctgcc tattcctcca cacctgagac accctctgct gctgttcgag 4620

aagaaacggc ctgagtggct gagcatggac ctggacggaa tgctgacagt gatcggccag 4680

ggactcgtgg gcatgacccc tagagatctg ggaacagtgg caggcgccac cgctcagatt 4740

cagcgaccta tccaggtgca gactggcggc agactcagaa gaaacctgca gagaaacctg 4800

aaggtgctcc aggtgtccgt gtacagcggc aacctgctgt cctttagcag acggggcatg 4860

aagtctagcg tgcggctgaa aaaccgcgag tgggagaaac ggaagcagat tctcggaggc 4920

ggcggaaagt acaaagagta cttcctgaag cgcatcctca tccggaaggc tatgacagtg 4980

ctggccggcg acaagaaagg actgggcaga ttcatgagat gcgtgcagag cgagacaaag 5040

gccgtgtctc tgcaactgcc tctgggaaga aacatggaac accggttgac catggacagc 5100

gccctgagcc tgacaaagag agagggaccc gagacactca aaaagaaata cctggtccag 5160

caccagatga agtgtctgac cgccgtggat tgtctgcagg ctttttggga catgggctac 5220

ccctacgatg tccctgacta tgcgtcc 5247

<210> 1138

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 4B poly-FSP

<400> 1138

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgatcc agaagaagaa gcagaagtcc 720

aacctggacc tgcagcacca gcgcagacgc aaaaagaaga aggacaaggt cgagatccgg 780

aatcggagcc ccaaggccaa tagcacagag tgtcaggggcg agcacagcag agagcagaac 840

ctgctgaatc tggtgcagct gaaccctcac agccctcacc acagaaagga cgaagaggac 900

caccagaagc ggcaccacca ccacaatcaa aagaaaatga gcgtgatggc caagaagtcc 960

cagcagcctc tcggacaggc ctttggctct ctgtgtcagc tggtgtccgc cgatttcagc 1020

gcccctcaag tgaccagact gcggagcctg aaccatctgc tgatcaacga gggcaacaac 1080

cactggccta gagtggaaat gcctaccggt tggctgctcg tgggctacaa caccagaaac 1140

acagcccaga atcctcggct gcagcagcac ctgaaacact ggcagcagtg caccagagcc 1200

agatggtgcc aagagccttg tcagaccctg accttcttcc atttcttctt ctttttcaaa 1260

acggtgtccc tgtgctgccc cggctggtct aaaggacaac ctcctccacc tggcctgaag 1320

ccttctagcc tggcttctca ggtggccaga accacaggcg tgtaccagca cgtgtggctg 1380

atctgcttcg tgttcttcgt gtgcgtggaa accggcttct gtcacgtggc ccaagtggga 1440

ctcgagagac tggtgtctct gagcggcatc accgacgtga accattgggc ccagcctaag 1500

ccatctccta gcaaaggcac cggcatgaac agacccatcg gcctgagagt tggcaaaggc 1560

1620

cggacaagag gaagccctct gagactgaca ctgtggcctc cacggaacat cctgaacagc 1680

ctgcctagca gcatggaaat cgccgtgctg caagagaacc gcaagaccag aaaggccgcc 1740

agagagaagc acagaaagac cgacaacctg cacctgtgct actaccacag ccagagcaac 1800

cggaacaaga gccggcagat ggaaagcctg ggcatgaagc tgcagagccc tctgtgggat 1860

tgctccacca caacaggcac acaccacggc tgtctgtggg agatccctag cgttacactg 1920

ctggcctgca gagtgaacgc cgtggaagaa ctgcacagca ggcggatgtc tgtgcggatc 1980

gtgaagaact gggtgtcctg ctgttacgtg cgcctgtctg ctgtgggcct gtctacttgg 2040

agcgccctgg acgtggtcat caacctgaag cggctgcaga agttcagcca gaagtctcag 2100

caccactgcg ccctgagaaa gatcgagtgg gccacaaagc tgagcctcgg cggaaaagct 2160

ggaccccagg ctcaagagaa ggccaacgcc aagaagatgc ccttccagcg gtggatcttc 2220

agcaatcagc tgaccgtgca gatgctgtgc ctgcctattg aagccctgac tgccctgttc 2280

acctgtcgga cagccagcat cagcgtgaac atcagcaccc tgatgagcat cctggaagcc 2340

cctctgtttt ttcacggcga cctgtgcgag gaaaagaaga acggccggat cagcagccag 2400

gctcctctca gactttggtc ttggtgcggc acaagccaga cctactttgg cagcaccgcc 2460

aagaaaagcc ccaccagctt ttgctgcgag tggggaggac aggctcaccg gcagagaaat 2520

accggaatgg aaatcctgct gtggattctg ctcaagatgg aaattcagat cttcaagatt 2580

tgcctgggcg agatgcagct gtgcacctac acaccccaca tctgcagaca cacctgtaga 2640

ggcggaccta gcgccagcga tagatgctgt agctgtagcc ctagcggctt cagcgccgga 2700

agaggcagat gtccagttca gggatgtctg cggcctcaca gagtgcagct gctgagaaga 2760

tggggccctg gatctcctgc tggccagaga ctgtctaagg gctttcagct gctccgttgg 2820

tggggaccag gatctccagc tcctgagcct agaaagggcc catttccacc acctgatcct 2880

ccatggcctg tgaccgctgt gacagtgatg gctggctctg tgcctagcgc tcagtctgtg 2940

gatgctctgg aaagccctgg acctctggca ctggaaggac ctagcagccc cagaaacctg 3000

ctctggcggg aaatgagcat cttcctgcct ggcatcttca cctctcggat tctgaacctg 3060

caagtgctga agaagatcct gcggagcttt atgaagctgt accactctct ggtcatgtgc 3120

ctgcggctgc ggaccaagct ggaaaaagct ctgagcgctc tgttcatctg gccccagcac 3180

agctacaagc agaggcctcc tctgcagcct gtgcaggtcg tgctcagact gggaaacctg 3240

cctccattct tcagaggcag cgaggtgccc ttttcctagac cttgccggac caagatgctg 3300

aaccagaagt gtctggcccc tttcatcaca cacccattcg cctctcagcc cctgggacaa 3360

gagatgccca acaacaccca gatcagactg ctgaagcccc tgctgacccc tgacaccaga 3420

tggatcttct ccaaccagct gcagacagtc cagatgctct gtctgccacc tctgaagcag 3480

ggcgagagaa tcatttggcc cctcggcggc tttcagatcc ccaacttctt ttggagcctc 3540

ggaatcggcc acactggcgc ctggtggcat actggaaggc ggagaagaga atggggcggc 3600

tccaccgtgt ctagaagccc actgtggatc agctccatga tctacctgag aggcatggcc 3660

ttcgccagcg acagctccat cagaaccacc agagtgtcct ggtgcagaag atgcggcgcc 3720

aaatctgccg ctgcctccag cctcaagaag aaaccccggg acatctgtcg gatcagcgac 3780

tctaagaaga tgacccagaa gcgcgagagc accctgggca atttctggat gaagctcctc 3840

cagctgccag acaagaagaa gaagtctacc gaaaagacca agatcatcaa gaagatgaag 3900

aaacggatca tggtccacat gtgcagctcc ctggctctgc caacaggcct gacatctctg 3960

atcctgccaa gcagcgatct ggccgtgctg atctctagct ccaccagcca ctttctgatg 4020

cggagcccag tgctgcctag ctccagactg acttgtgcct ctccacagct gcccagaatg 4080

tggacctggt ccagctggct gaagtggctg aaaaactgga agcggttcaa gcttaaaaag 4140

aagtacatcc tggccatgac gctgaccctg aacgggcatc caagaaccgc ctctatcacc 4200

ctgcctacca ccagcctgaa gggctctaga accagctgtt gccccagatc tcaccccaga 4260

ctgcctagga caaagtggcc tctgcagtgc ctgctgcaca gcctgggaac aactggaaca 4320

ctcgtgtggg aaaggggcgt gctgcagatg agaagtctgg aagctcactg ccctctgcag 4380

accttccacc tccaccactg tagaactcag cccagctctt gccctcctgc atggattctg 4440

taccaaggac agtggagcgg agccgtcgtg ggacttagac ctgtggctca ccatctgcca 4500

agacctattc agggcctgca ctgcccacag gctccacatc aaccagctct gtcttggggc 4560

aagtggcccg tgggaatgac aggacctgcc agatgttgcc ggtggcctag acgggaactc 4620

aaaaagaatc tgctgctccc ctgccggtgc agacgcgagt atagagtgac aatgggccaa 4680

gccttcggca gctttagcgc cgactttagc gctccccagg tgttgaagaa gaatcggaag 4740

cagaacaaga ttatctgcaa gggccaccgc atcacaagcc tgatcctgag ctttcagcgc 4800

gtgaaactta agaagaaagt caacgcctgc tctcccgcct gcacctctaa agcctggact 4860

cccagaaagc tcgtggggag agccgtcaga agaaagggca tcatctgtat caagaatatg 4920

ttcctgcagc ggaagggttt cctgaacctg agccacgtgg aaaagttcgt gcctccttct 4980

ccctcctctgg ctctcggccc tagaatgcag ctctgtaccc agctggcccg gttcttccct 5040

atcacacctc ctgtgtggca catcctggga cctcagaggc acaccccttg tcaaaagaag 5100

gtgccctccg ctagcccatg gggccttaga ccatctcctt gcctgagctg gtcctccaga 5160

attgtggccc ctggacacgc cctgagctgt cggactagaa gaagatggtc cagcaagggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1139

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA Cloning Cassette 1A

<400> 1139

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaaccgag 120

atccagctga ccatgaacga cagcaagcac aagctggaaa gccccgctct gaaacaggtg 180

tcaccagcct ctccacctac tcagcagcct cagacacctc aggattctag acaggtgctg 240

gccctggcca agaaaaccct gacaaatgcc tccagagccg gacagtttcc tgtgtggcgg 300

agaacaagca gaggcatgag cctgcagtgg aagtccggca atgcctgcac acctctgaga 360

tgccctagca gaagccctgc cacattcctg acagccctga atggctggca cagatctggc 420

ctgagagccc agatgcctag ctcttggtct agcagcggca gaacactgaa gctggcccct 480

ctgcaagtga ccaccactct gaagctgatc ctggtcatgc tggaaatcgt gaccgagcgc 540

tccctgctgt actccagaat gtgtctggtg cagatcccca cacacacctg ggagcccgat 600

tacttcgtgt ccctgcctat gatgttcagc tgggccacac agtcttttcc cgtgggcgcc 660

gtgtttctga ccagacctgc cattccttgc tgcagctgta ccagccacag caacccttgg 720

agccagagca atcagcagct gaagtggacc cctcacatcc tgaagtccgc cagcttcgac 780

aagaaaggct tcgtgatcct gagccggcgg agcaagattg aggcctctcc tatcggctct 840

atcgccagct ctcctggaca tcctctggga cctacactgg ccatcagatt catcagcgcc 900

cctcagccta gcagctctac agtgacagct gcccctagcg gccagtactt tatcgccaag 960

aagcggatca agctgcggcg gaacaatatg ggcggcaaca gatccatctg cctgaccaga 1020

aagcgcaaga gagagaagcg gagccagaag tccaggcaga accatctgca gctgaaaagc 1080

tgcagacgga agatcagcaa ctggtccaag ctgagaagcc cctggatctt caccaagtgc 1140

gagatctgcg tgaagaacac cgccatcctg gctatcaccc tgatcacccc tcaggaccct 1200

catctggacc ctgtgtgtca ccctcataga aggcctggat ggaagggcca ccatagagtg 1260

gtgttctctc accatcctag cggcagcaca aagagccact ggcagcaaag agaacgcgga 1320

ggcggcagac gcgagaagaa gggatgtaga gaacagcgga aggtggtcaa gaacccctct 1380

cagcccgtgc ctctgagaaa cgatggcatc accaacggca acgactccac cagaagctgc 1440

tccatcagcc tgaagaaaag cctggaaaag atgcccctga tgcggagagc cagacctacc 1500

agctgtagca cctgtagaat gtggtggaag ggcaagaaac ggcggaaaga aatcgtgcct 1560

1620

gatgtgtcta gcatcagcca ttggcggctg ccctgtaaag tgaccgagag cagctccagc 1680

ttcttctaca ccagcgacag gacccctctg agcatcagca gaccaaccag cagagctaga 1740

cagagctgtt gtctgcccgg actgacacac cctgctcacc aacctctggg cagcatgagc 1800

actagaagag agatgccaag ccaggccaca ccttctccac ctggaccttg gagagccgct 1860

ctgggcaaag aagtgaagag atcccagagc aagctgcgcc tgagaaacac aagccctggg 1920

gagaagaacg atctccagct gttcgtgatg agcgacacca cctacaagat ctactggaca 1980

gtgatcctgc tgaacccctg cggcaacctg cacctgaaaa caacatcact gcagcggcca 2040

cctcggagca tcctgtctct gaatggaatc tgggaagtga tgccccggac actggaaggc 2100

tgcatcagcg tgaaggatca cagaaccagc cagaaccaca gcaagtgcat caccgaatgt 2160

gtgcggactg ccctgaacgt gtccaagaag agactgccta agctgatgaa gaagaaggac 2220

agcaccatct tcatcaacaa agtgcgccag aacttcaagc gcaacttcaa gaagaagaaa 2280

cccctcactt tcagccccag cagacggatg actcggagaa ccatgacaac caccaaaatg 2340

atgatgatgc ggcggaccaa gcggagaagg cgcaaaaggc ggagaatgac catgatgacc 2400

agacggaccc tgctgacaaa gaaaacctgt ctgaccccta gaaccagcgc cgccacaaaa 2460

gctctgccta gacagaccat cagccagcgg aggcctattc agaccccacc tggaacatct 2520

ctggccatct ggggcagaac aagactgacc ctgacaggca cggatccag cggaacaaga 2580

gcctctcctt ccgccgtgtg cctgtgttgt tggacctgtc caagcagaag cttcccagcc 2640

gcttctccta gtggactgag agcacctgtt ggagcccagg ctcctcctga agtgacactg 2700

agagcctctt gtgcctctag cgcctctaaa gcctgtgctg gactttggcc ttggggagcc 2760

caagttcgag ttggagctgg aacaggcggga ggacctgctt gtgttcctgt ggctgaagct 2820

tctacaggcg catggacagc tgaaggcgcc gctggaacaa gatgtagagc ctctagagcc 2880

ccttgccgcc aacttcctgc tagctcccct accaagtgtg aaatttgtgt gaagcgcaga 2940

cggcggagaa agagaagcag ccagtctaga agccccgtgc ctaaaaagcg cgagaggatg 3000

atgctgcctg tgcggggccag aacagctaga tctgcacttc ctcaggggcag agcacctctg 3060

gaagggatgc tgcttcctag agcactgcct agcctgctga gcagaatgcc tgccagaaga 3120

3180

tgtcatcctc tgcctcaact gtggggccct ccacctcctc atcaggcccc aatgacactg 3240

aaggccctgg ttaagacctg gacacagttc cgcaactacc ccagagccga caagaagaat 3300

gtccggtcta ccgccttctt cctgctggac atcatcctgg gcgattgccc aagaacaatg 3360

gccggacctt ctgcctctct gcatcctgca tctggcagag cctccagaaa gacctggcag 3420

agaaggctga gagcttgtag agcaggcgga cctgctggat gggctagaca ggcctatcct 3480

agactgaaaa ccttcggcgt gcccctgggc tctattctgt gtctggccgg cagcctgtcc 3540

acaatgatga tcattagcac caccagacaa gagtgcggca ggcgggcttc tgtgccttgt 3600

agacctatga tcggaagcgc cagaccagga ccttggcgga catctgctat gcctagtgcc 3660

atgggagtcg ccctgcctac ctcttgtgaa agcggaagat ctcctcctgc caccggcgga 3720

agaatgccag gatctcagtc tatcatgatg agctggatgc ctccactggc tccatgtgcc 3780

gcttgtccat gttctcccgc tcctacactg tgcccagcac atcctgctag agcaccaaca 3840

gccgtgcctg cctttacacc tctgtctgct catcctgtgc cagtgctgtc tggatgtcac 3900

ctggccgtca gaacctccat gctgacactg ctgcccatgg aagccaaaaa gagcatgaag 3960

cggtccaacg ctaagaaaat ggaagatatg atctgggaga gccggctgcc actgaggcca 4020

caaagaggac ctagacctct gcacagatgg ctgcagcaga caaccggctg tagccagagg 4080

cggatcgtgt tctttctggc cccatacttc cctcggcacc ctcacagatc tccaccaaca 4140

tcttgcccac ctctgctgga agctttccca ctgccaagag cttctctgag ggccacagtg 4200

ccaagacata gctctgttct cgccaccggt ctgcacctcc acagacagat ttctctgccc 4260

ctgagaggat tcctcctgca ccaaccactg ccaggacagc atctgtggct gtctcacaga 4320

ctgaggctga gactgcccag gacacagcca tctttgcctg gaccatgtgc aagcctgctg 4380

tccacactgt ctcagccacc acctcaggct ccatctcaag tgtggactgc cgctacactg 4440

agatgtcctg ctgttcctgc agctgcctgt cctcctgctc tgagatccat gcctaagagc 4500

cggaaccagg tgctgttcag acggaatctg accccaagcc agctgagaac actggcccca 4560

ccttatatgc tgctgcccca gtctctgtcc cagagcctga gtagaaagca gatccctagc 4620

cagtccatgc tcgtgggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1140

<211> 4672

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 2A cloning cassette

<400> 1140

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaagaaag 120

atgcagagcc tgaatcaccc cagcagactg accaatggct ctcctccact gcacctgtgc 180

cagcctctga gaatgagcag aacccctcct agaagagtgt gcgccgctcc tccacataga 240

ctgctgggcc cttccagatg gaaccctcgg agaaagacct ggaagatgcg gaagaagtac 300

gcccagaagc ctagccagaa gaaccacctg tacggcggca agaagcgctc taagttcagc 360

agaagccacc tggacttcag cccctactgg atgaagaaag tgaaggtgct gttcaaggcc 420

gaccagagcg agtctagcct gagcagcgga agatgcagcc tgatggaaca gatccctcac 480

ctgaagtctc ggcggaagtg gcagatcaga cacgtgctga agaacctgtg gctgcccatg 540

agaagcctgg gagaaccttg gagagccggc tttcagtggg gagagtctcc ttggcaagtg 600

atggcctctc tgaccggcag agccacattt ctgctggccc tttgggaatg tagcctgcct 660

caggcaagac tgtgcctgat cgtgtctaga accctgctgc tggtgcagag cgggaacaag 720

aaaacaaagc tgtggttcag cctgatcaac atccaccacc ggaagaaccc actgctgcct 780

atgagatggt ggtgtaccag aggcggcgga aagcggaaaa agaaaatgtg caaggtgtcc 840

ggcggctgca ccgcctgtgt tgagagatgt ccttggaagg gcatctgctg gaagtggcct 900

gtgacagtgg ccgccattag aatgtgtgcc ctgtctcacc ctagcttcta catcagcagc 960

accgtgtgct ttctgagcaa gcactgcatg accgcctgca ccagaaagag aatgaagacc 1020

ggcgccatcc aagagaaggg cgctattcaa gagaaagggg ccatgaccaa gcggatctgc 1080

gtggtcaatt tcgaggcctg cagagagatg gaaagcctga gcgcccctga gaagatcacc 1140

ctgtttgaag cccacatggc ccggtgcacc tccatcaatg tgctgtgtgt gcgggccagc 1200

ctgattgaga agctgatgaa agaaaagcgc aagatgaagc ggaaccaggt ggcctctcca 1260

cagatcatgc agcggctgtt catcaagggc taccagaaca accacttcac cttccatctg 1320

caaaacttcg ccttcttcag ctccttcggc aacaacaagt acatcaatat cctgaccatc 1380

ctgttccacg tgcggaacat cgtgtacggc atccaagtga tgctgtgcgc tagccctcag 1440

attatgcaga gaaccagcgc cgaccggttc cggatgaaga gaagaatgca gaatctggac 1500

aacctgggcg tgccatggcc tctgttgcct ggaatcccat ctctgaacgc ccagtggatg 1560

ttcctgagaa tcgcatcccc gcaaatcatg cagaggatga gcgccgtgaa cttcgagaca 1620

atcggggcca gaaaccggaa catgaagaaa accatgagcc acctgatcgc ccatcaggcc 1680

ctgaagatga gcggcctgag actgtttcac cccagactgc tgacacggaa gaagccggga 1740

aaactgctgc tcgcccatat gtacggcttc agcacctacg gcacccagaa aggcgagtgc 1800

gtttgtctgc agtgtctgag cggcagcagc ctgttcattc tgaagcagcc cctggacctg 1860

aagatcagca agaccttcat gggccacaga gagggcagcg agtctgataa tctgggcctg 1920

ctgtatagct tccctccacc ttatcctatc agcgagggcc tgaccatgac caccggcaga 1980

aacatcaaca gcaactccct gatgaccaac tgcctgttca gccccatctt cctgtgccac 2040

cacgccatca tgcacagaaa gatgacaagc cctcacttca ggctgttcag cagcaagatc 2100

ccacatcctc aggtgccatc tgtggtggcc ctgtgcaagt tccggggcctt catccacaca 2160

cctagaagcc ctggctgcag cgtgtacgcc aatatgtgct ggcccaagtt cttcctgacc 2220

ttcagccagc agtggtccag cgtgacatct ctggaatgga cagcccaaga gagaggctgc 2280

agcagctggc tgatgaagca gacatggatg aagtcttgga gcctgcggga ccccagctac 2340

agatccatcc tggaatacgt gtccaccaga gtgctgtgga tgcctacctc taccgtgctg 2400

caggctcatc cacaagttcc agctctggct cctcaagcct ggtggcctgc tagaagagga 2460

cctctgacat ctgccccatc cgctcagcag tccctgacca gagagaagat tatcaacccc 2520

accatcagct gcccctttca gagccccacc aagagactga tctaccctcc tagcacctgt 2580

tctctgccct gcagaggctt cctgcctagc agatttcctt ggggcccacc tcctctgaac 2640

agcgtgatcg tgcacaagat cgtgctggtg ttcctgaagt ccctgtccag ctacaactgc 2700

gagcggagaa tcagcggcat ccagaagaga gccatcgagt tcgccgtgct gaaactgctg 2760

aatatggacc tgcctctcga gaacacacag atccccagaa caagcccact gtggaagatc 2820

acaatggtgc tgacataccc caactgcgtg gtgcatgtgc tgatgtttgg cgccctgaga 2880

gccacagctc ctgctacagc tgcacctcag gctcagaccc ctcattggag agctgccaca 2940

acaagcagca tgagcagcag ccctagagcc gagcctacat cttctagctc ttgggccacc 3000

tgttggccca gatgctgctc cagctcttgt tactggtcac tgtctagctg gcctccagct 3060

ggcgccgctg aagctacaaa taccagaacc agaagccgcg agagccagag gggtagaatg 3120

ctgacttgga gaagctccct gtggttgcag ggcaccagat gttttacagg cgtgcggacc 3180

agctccagat acaagaaaag aggcgtgatc gcctggcggga gacagatcaa tagacggaag 3240

ctgcagagaa agaagcactg gaacagcgcc tacctgggca gaacactgct ggtcacctgt 3300

atcctgtatc accggtggat cgtgacctcc atgtgtcagt cccagagatg gctgaccagc 3360

accacaagta gatctagcgc cctgatgtgg accgagctga gcctggaacc atggattccc 3420

tacctgcatc agcagaaaac ccagcggaca ctggaatccg ccggactgtt tccttggctg 3480

cacacccagt gccaagagaa gaaaagcaga aagctgatgt ccagctgcac acacccctgc 3540

acaatgctgc tgccacaggg atgtcatcct gcctggctga gccctatctc tgcctgtcca 3600

agactgctgc catatccttt tcacgtgctg gctctggaag tgacctttcc tcctggaacc 3660

gccatgcctc agatgcagtt gcaggctctg gctatgtggg agaaaggctg ggataagcct 3720

ggcaccagcc tgcagtgcta cctgaacaga agaagaaaca gccggaagca gagccaggtg 3780

caactggcca ttcaggtgct gctcgtggac aagcacaagt actttctgtt cggccaaaag 3840

aaaggccaca tgatctttag cctggccaac ggcctgtggt ttaacctgat tttcaactcc 3900

cggaagaaac tgctcgagga catgaagcaa aatggcttca ccggcaaagg cctgacacac 3960

cccgtgcctc tgaagcggac tctgcagaac aaaagaaaca agaatatcaa acgcgtcaac 4020

cagaagaagg gcaacatcaa gcggaagtcc atcctgctgc acctgattgt gctgaaatagc 4080

cccgagagca acctgatcct gatccagcat ggcggcatct tcacctttcg gttctgggtc 4140

gagaagctgt cctggaacat ctgcaagaac ctgagcctgt acctggacaa gttcgtgcag 4200

agactgctgt acgtgtacat catcgagaca aacgtgttcg tgctgaacct gtttcatgtg 4260

ccgtgaacc agatcttcat gatggccttc tacctgaagt acaccgagaa ggcctgggtc 4320

atggaactgg aatggctgat ccaacagcag tgctatcagg gcagctgcgg cagagcttgt 4380

4440

gccttctgga agcctcctag ccagggcatc tctagctgtc tgatgaccgg ctgttggctc 4500

gagaagaaag ctacctggat gcggcggacc cgccggaaga agagggaaga tcattgcgga 4560

ctgagacacc ccagctggcc ttggacaaga tgtctgagga tgaggcctcc aagaagcggc 4620

tacccctacg atgtccctga ctatgcgtcc taaaggcgcg cctagcggcc gc 4672

<210> 1141

<211> 4687

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA cloning cassette 3A

<400> 1141

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaggagct 120

gctgctgcct cttgtcagtc tcctccttct cctcgggaaa ccacacctag ctccgacgcc 180

accagacgga caagacctat tgccgctcag agcaagaaaa ccatgacagc cgccggactg 240

300

agatgtggcg gctggctgtg tggctggaca cctagaggac agaacaccag aagcaccaac 360

ctggaaaaga accccagcct gaagaagcag agccagcgga gaggaaagct gagcagcctg 420

ttcttcagcc actgcctgtg ccggaacagc agaagggcca cacctacatc taagggcagc 480

ctgcggcgga agtacctgag agtgcctatt cctccacacc tgagacaccc tctgctgctg 540

atggacctgg acggaatgct gaccgtgatc ggacagggac tcgtgggcat gacccctaga 600

gatctgggaa cagtggctgg cgccaccgct cagattcaga ggcctattca ggtgcagacc 660

ggcggcagac ttacacctcc tcctctgatt ctgtggcctc aggccgctcc tgttgatcct 720

cctggctctg tgtggattct gccctgcaag aacaagaagc ggagaaagct gcggcccaag 780

cggctggact ttcaccctag atcttctagc ggcgtgtccg tgatcaccat gaagctgtgg 840

gagaagcact tcaagggcct gaactggaac ctggtcgtct ggattctgaa cctgaagatg 900

atgaacatcg agatcacccc tcagcggagc aaggccagaa agtccggcgt gatgaagtgc 960

tgggatctgt gccccagcga gaagaagagg aagctgcctc tgcctctgac cttcagcacc 1020

ctgctgtggg actctagaca tagaggcgcc agctccagca ccagcagaga ctccagaagc 1080

tttttcacca gcgctcaccc cagcagaccc tgcagaatga gactgtacca gttcctgatc 1140

cacctgagaa aggtggacac ccaccatctg caggtcgtga ccaagaccgt gacagccaca 1200

acagctgacg gccagagaat gcggagactg aagtgcaagg cctgccggct gagatgggct 1260

agacctgaac ctgctgctca ggcccctcca aagagccact ttccacctca tcctagcggc 1320

cctagcagaa ccagttctct gcagctgcgg agcatccctc acgtgccaaa agccacagcc 1380

gctcacagcg tgacagtgaa gctgcagaag ggaaagaaca gcccctgctg ccaaaagaaa 1440

ctgcgggtgc agaatcaggg ccatctgctg atgatcctgc tgcacaatac ctacatggaa 1500

atgcactacg gccatcaggc cccacaggtg gccatgctgc ctaagctgct gtgcagacag 1560

atgctgccca ctctgagcac actgcagcaa agacacccac agggcccttg tcctccacct 1620

gtgacaccac tgagagtcca gtctgtcctg ctgctgggag tgcctcagac agctgctaga 1680

cagcctagca gacccaccgg cagaacccct accacaagac tgatggcccc tgtcggcagc 1740

gtgatgagct gttctctgct gacccagcct tctccagcca gcgcctgtac aatgcctcca 1800

ctgctgcatc ctcagtgggc cccacattct gctggattgt ctcccggcgc ttgcagacct 1860

gcctgtacct gtatcagcat gaccaccatc tccagagcca cctggcaggt ttccgtgtac 1920

agcggcaatc tgctgagctt ctccagacgg ggcatgaaga gtagcgtgcg gctgaaaaac 1980

agccggcgga acagacggta cagaaagatc tacggcgagc tgaagatgtc cctgcaggcc 2040

gtggctccta gatgtcctag atctgccgtg gacatcagcc ccggacctac ctctcctagc 2100

ctggcctctt gtgcctctgt ggccattgga aaactggccc tgcttggcgc ttctgcccct 2160

tgtggtgctg ctggcgctag agaggctgga agatttgccg tgctgggcac catggtcatg 2220

aagcacctga accgcaagct gcatctgacc cggtcctgcc tgatcgatct gaccatgatg 2280

atgagccaga agctctggaa gaaccagcgg aagagaatcc ctctgccatc tcctagacag 2340

catctgctcc ctcagtaccc tctgcaccct cctccacctt gtctgctgct gctccaccac 2400

agacaccacc tggcctttct ggaaatggcc tgcagcagcc agcacaccca ctccatcaag 2460

atctggatca gctttagcca cgagcagagc agcccattcc tggccagatc ctggaacacc 2520

atgcagtgga acaacaagaa aatggccatg acagtgatcg ctggcgccca ccactgtcag 2580

tacaagctga cctacaccaa agtgtggaag atgaattgga agaaaatgat gatcggctcc 2640

cagcacctcc tgttcagccc tctggatagc tctccactgc aactgctgct tcaccctcac 2700

ggcaagagat gcaacccttg ctgcagactg acatggatgt ccatgctgca gaaacagtgc 2760

tgctacaacc tcgtgatcaa gcacccactg atcttcagaa tcaacctgcg gagacaggggc 2820

aagaccaagg atggcgttgt gcagagcctg ggcttcctga accagcagat catcctgcag 2880

aaagagttct tcatctgcct gctcatgttt atcaagcggt ggaagcctgc cgccaccaag 2940

agagagtggg agaaacgcaa gcagatcctc ggcggaggcg gcaagtacaa agagtacttc 3000

ctgaagcgca tcctgattcg gaaggccatg actgtgctgg ccggcgacaa gaaaggcctg 3060

ggcagattca tgagatgcgt gcagagcgag acaaaggccg ttagcctgca gctcccactc 3120

ggaagaggcc ctgtgtacta cgtgtgcaac cggatgaacg tgtccagcgt gctgaagccc 3180

3240

aatgtgtcct ctgtgctgaa accctgtctg atccctgtgc caatcgacct gcctcggatg 3300

ctgccaatca gcagcccact gcacagaccc cagatgccat tctggacctg cacctttcct 3360

gccacaacat ggggcacctg ggagacacct tctacatggg gctggcggac ctttcggggc 3420

accagagtct gcagaaaagc 3480

caagtgttcc agtgggtcct gcggaccgag aacatccgga agctgatctg gaccaagcag 3540

cggagcttcc tgtctctgtt ccccaagctg agagccaccg aacagatgac caatgtgggc 3600

tgcgccttca gcggccggat tctgcatcaa cctgaaatgg ccgccatgcg gctgatgccc 3660

aagttcctga atagcaccaa cagctggtgg acctgtacca acctgagcgt gcccatgatg 3720

ctgaccattc tgatttggaa gcgggtgttc attctcctgc tgtccgacaa gaagctgctg 3780

aagaagtcta gccagctgag gcggtcttgg agaaacgtgt cccggatcag ccaccagatg 3840

aagtgtctga ccgccgtgga ctgtctgcag gccttctggg acatgaacat ggaacaccgg 3900

ctgaccatgg acagcgccct gagcctgaca aagagagagg gacccgagac ttgcaagaag 3960

aagctcctgc tgaaactgct gacaatgagc ctgagcaaca tcctgatgaa gttccggctg 4020

cggctgagaa tcccaagcct gaggaagtat cgccagcatc tgaaaaagac cagcgccaag 4080

ggcgagaagc tgaagcactg ctttaaggct tggggcagag gcccacctct gcacacaaga 4140

agccagaaga aagaccctgc cggcaagctg aaaaaactga cccacactgag cttcgtgctg 4200

atcgaggaac ggcggaacct gcagcggaat ctgaaagtcc tgtggctcaa gaaaagcacc 4260

aagagccggt atctgttctt cctgaccgtg tgcggcttct tcgagaagaa acggcctgag 4320

tggctgtcct ccagaaagaa gggacacaca aagaatcgga ggaaggccaa gcggaagccc 4380

cagatcgtgc acaagaagag aggcctgttc ctcaaaaaga aatacctggt gcagggcgcc 4440

aagaagatca ccttcctgtc caacggcatg gaagcttgcg gcacacaccc cacaccaaga 4500

ctgagcgaca gagtgttcct ggtcaagatc accaccacca caagcagccc cagaagaagc 4560

ccagcactga gagctagagg cggcaccaca attggcgaag tgaccagcct gtgcttcttc 4620

tacctgctgt acggctaccc ctacgatgtc cctgactatg cgtcctaaag gcgcgcctag 4680

cggccgc 4687

<210> 1142

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 4A cloning cassette

<400> 1142

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaatccag 120

aagaagaaac agaaatccat ggccaagaag tcccagcagc ctctggtgct gaagaagaac 180

240

ttccaagagg aaaagaagaa cggccggatc agcagcagcc tgatcctgag cttccagaga 300

gtgaaggaca gcaagaaaat gacccagaag atgtgcagct ctctggccct gcctacaggc 360

ctgacatctc tgatcctgcc tagcagcgat ctggccgtgc tgatctctag cagcaccagc 420

cactttctga tgcggagccc tgtgctgcca agctccagac tgacatgtgc ctctccacag 480

ctgccccgga tgtggacatg gtctagctgg ctgaagcacc ccagaaccgc tagcatcaca 540

ctgcctacca caagcctgaa gggcagcaga accagctgct gcccaagatc tcaccccaga 600

ctgcctagga ccaagtggcc tctgcagtgt ctgctgcaca gcctgggaac aacaggcaca 660

ctcgtttggg aaaggggcgt gctgcagatg agaagcctcg aagcccattg tcctctgcag 720

accttccatc tgcaccactg cagaacccag ccaagctctt gtcctcctgc ctggattctg 780

taccaaggcc agtggagcgg agccgtcgtt ggacttagac ccgtggctca ccatctgcct 840

agacctatcc agggcctgca ctgtcctcag gctcctcatc aacctgctct gagctgggga 900

aagtggcccg tgggaatgac aggacctgcc agatgctgtc ggtggccccg cagatctcag 960

aagtctcagc atcactgcgc cctgcggaag atcgagtggg ccacaaaact gtctctcggc 1020

ggaaaagccg gaccacaggc tcaagagaag gccagacctt ctccttgcct gtcctggtcc 1080

agcagaattg tggcccctgg acacgccctg agctgtcggga ctcgtagaag atggtcctcc 1140

aagtgcagag gcggccctag cgccagcgat agatgttgta gctgtagccc cagcggcttc 1200

agcgccggaa gaggcagatg tcctgtgcag ggatgtctga ggccacacag agttcagctg 1260

ctgagaagat ggggcccagg atctcctgct ggccagagac tgtctaaggg cttccaactg 1320

ctgcgttggt ggggacctgg atctccagct cctgagccta gaaagggccc atttcctcca 1380

cctgatcctc catggcctgt gaccgctgtg acagtgatgg ctggctctgt gcctagcgca 1440

cagtctgtgg atgccctgga atctcctgga cctctggctc tggaaggccc tagcagccct 1500

agaaacctgc tgtggcggga aatgagcatc tttctgcccg gcatcttctg ccaaaagaag 1560

gtgccatctg ccagcccctg gggacttgtt cctccttctc caccactcgc tctgggccct 1620

agaatgcagc tgtgtaccca gctcgccaga ttcttcccca tcacacctcc agtgtggcac 1680

atcctgggac ctcagagaca cacacctcag gtggtcctga gactgggcaa cctgcctcca 1740

ttcttcagag gcagcgaggt gccctttcct agaccttgcc ggaccaagaa cgagggcaac 1800

aatcactggc ccagagtgga aatgcctacc ggctggctgc tcgtgggcta caacaccaga 1860

aacacagccc agaatcctcg gctgcagcag catctgaaac actggcagca gtgcacacgg 1920

gccagatggt gtcaagagcc ttgtcagacc ctgaccttct tccacttctt cttctttttc 1980

aaaacggtgt ccctgtgttg tcccggctgg tccaaaggac aacctccacc tcctggcctg 2040

aagccttcta gcctggcttc tcaggtggcc agaaccacag gcgtgtacca gcacgtgtgg 2100

ctgatctgct tcgtgttctt cgtgtgcgtg gaaaccggct tctgtcacgt ggcacaagtg 2160

ggcctcgaga gactggtttc tctgagcggc atcaccgacg tgaaccattg ggcccagcct 2220

aagccatctc ctagcaaagg caccggcatg aacagaccca tcggcctgag agttggcaaa 2280

ggcgtgtggg ccagactgct caagaagaag cctagagaca tctgtcggat ctccaacaga 2340

aagaccggga aggccgccag agagaagcac cgcaaaacag atagagccag cgtgtccttc 2400

tggactctgc tgcctacaac aggcgtgcgg acaagaggaa gccctctgag actgactctg 2460

tggcctccta gatgcagatg cagacgcgag tacagagtga ccatgaacat cctgaacagc 2520

ctgccaagca gcatggaaat cgccgtgctg caagaaaccg ccaagaaaag ccctaccagc 2580

ttctgctgca acctggacct gcagcatcag cgcagacgca aaaagaagaa agacaaggtc 2640

gagatccgga atcggagccc caaggccaat agcacagagt gtcaggggcga gcacagcaga 2700

gagcagaatc tgctgaatct ggtgcagctg aatcctcaca gccctcacca ccggaaggac 2760

gaagaggacc accagaagag acaccaccac cacaatcaaa agaagatgag cgtcccactg 2820

ttcttccacg gcgacctgtg tagagagagc accctgggca atttctggat gaagctgctc 2880

cagctgcctc agaggcctcc actgcaacca gtgaccggaa tggaaatcct gctttggatt 2940

ctgctgaaga tggaaattca gatcttcaag atttgcctgg gcgagatgca gctctgcacc 3000

tctcggattc tgaacctgca agtgctgaaa aagatcctgc ggagctttat gaagctgtac 3060

cacagcctcg tgatgtgcct gcggctgagg accaagctgg aaaaggctct gagcgccctg 3120

ttcatctggc ctcagcactc ctacaagatc acacacccat tcgccagcca gccactggga 3180

caagagatgc ccaacaacac ccagatccgg ctgctgaagc ccgacaagaa gaagaaaagc 3240

accgaaaaga ccaaaggcca ggctttcggc agcttctccg ccgatttttc agcccctcaa 3300

ggacaggctt ttggctccct gtgtcagctg gtgtccgccg actttagcgc cccacaagtg 3360

accagactgc ggagcctgaa ccatctgctg atttggctga agaattggaa gcggttcaag 3420

ctcaaaaaga aatatcaggc ccctctgcgg ctgtggtctt ggtgcggaac aagccagacc 3480

3540

ctgagccacg tggaaaagtt cgagcttaag aagaatcttc tgctccccgt cgtgatcaac 3600

ctgaagagac tgcagaaatt catgctgaac cagaagtgcc tggctccatt tcctgcctgc 3660

accagcaaag cctggacacc cagaaaactc gtgggcagag ccgtgcggag aaagggcatt 3720

ctggccatga cactgaccct gaacggaatc gaggccctga cagccctgtt tacctgtaga 3780

accgcctcca tcagcgtgaa catcagcacc ctgatgagca tcctggaagc catcatcaag 3840

aagatgaaga aacggatcat ggtgcaccgg tggatcttct ccaatcagct gaccgtgcag 3900

atgctctgcc tgcctctgct gacccctgac accagatgga tctttagtaa tcagctgcag 3960

acagtgcaaa tgctgtgtct gccacctctg aagcagggcg agagaatcat ttggccactc 4020

ggcggctttc agatccccaa ttttcttctgg tccctcggca tcggacacac tggcgcatgg 4080

tggcatactg gtagaaggcg gagagagtgg ggcggctcta ccgtgtctag aagcccactg 4140

tggatcagct ccatgatcta cctgagaggc atggccttcg ccagcgacag ctccatcaga 4200

accccagag tgtcctggtg tcggagatgt ggcgccaagt ctgccgcagc ctcttcttct 4260

ccactgtggg attgcagcac cacaaccggc acacaccacg gatgcctgtg ggagatccct 4320

agcgttaccc tgctggctaa cctgcacctg tgctactacc actctcagag caacagaaac 4380

aagagccgcc agatggaatc cctgggcatg aagctccaac ttaaaaagaa agtgaacgcc 4440

tgcagcacct acacacccca catctgcaga cacacctgtc gcgtgaacgc cgtcgaggaa 4500

ctgcactcta gaaggatgtc cgtgcggatc gtgaaaaatt gggtgtcctg ctgctacgtg 4560

cggctgtcag ctgtgggact gtctacttgg agcgctctgg acaacaagat catctgcaag 4620

ggccacagaa tcaccggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1143

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA Cloning Cassette 1B

<400> 1143

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaaccgag 120

atccaattga ccatgaacga cagcaagcac aagctggaaa gccccgctct gaaacaggtg 180

tcaccagcct ctccacctac tcagcagcct cagacacctc aggatagcag acaggtgctg 240

gccatgccta gcagctggtc tagctctggc agaacccagc ggaaggtggt caagaatcct 300

agccagcctg tgcctctgag aaacgacggc atcaccaacg gcaacgactc caccagaagc 360

tgcagcatca gcctgaagaa aagcctggaa aagatgcccc tcatgatgaa gaagaaggac 420

agcaccatct tcatcgtgcc catgagcctg gcctgtagca tcaccgtgtc tttcgtgggc 480

cagatcagca cactggtgct ggatgtgtcc agcatctccc actggaacaa agtgcggcag 540

aacttcaagc ggaacgccga caagaagaac gttcggagcg gcgagaagaa cgacctgcag 600

ctgtttgtga tgagcgacac cacctacaag atctactgga ccgtgatcct gctgaacccc 660

tgcggcaatc tgcacctgaa aaccaccagc ctgggcgact gtcctagaac aatggccgga 720

ccttctgcct ctctgcaccc tgcatctggc agagcctcta gaaagacctg gcagagaagg 780

ctgagagcct gtagagctgg tggacctgct ggatgggcca gacaggcctt cgataagaaa 840

ggctttgtga tcctgagcgt cggagccgga acaggcggag gacctgcttg tgttcctgtg 900

gctgaagctt ctacaggcgc ttggacagct gaaggcgccg ctggaacaag atgtagagct 960

tctagagccc cttgcagaca gctgcctgcc agctctccta ccaagtgcga gatctgcgtg 1020

aagcgcagac ggcggagaaa gagaagcagc cagtctagaa gccccgtgcc taagaaacgc 1080

gagagaatga tgctgcccgt gcgggccaga acagctagat ctgcacttcc tcaggggaga 1140

gcccctcttg agggaatgct gcttcctaga gcactgccca gcctgctgtc tagaatgcca 1200

gccagaagag agagccggtt ttggctggct ctgcaggcac ttggactcag acctccacat 1260

ctgcctagct gtcatcctct gcctcaactg cttggccctc cacctcctca tcaggcccct 1320

acagctttct tcctgctgga catcattctg cggctgccct gcaaagtgac cgagtctagc 1380

aatatgggcg gcaacagatc catctgcctg accaacgcca agaaaatgga agatatgatc 1440

tgggagagca cgcccctgag catcagcaga cctacatcta gagccaggca gagctgttgt 1500

ctgcccggac tgacacatcc tgctcaccag ccactgggat ctatgaccct ggccatcaga 1560

ttcatcagcg ctcctcagcc tagctccagc acagtgacag ctgctcctag cggccagtac 1620

ttcaagggca agaagcggcg gaaagagatc ttcaagaaga agaaacccct gacattcagc 1680

cccagcctgg ccaaaaagac cctgaccaat agcagcttct tctacaccag cgaccgcgag 1740

gccaagaaat ctatgaagcg gagcatgacc ctgaaggccc tggtcaagac ctggacacag 1800

ttcagaaact accccagagc ctcctctcct ggacaccctc tgggacctaa tggcatctgg 1860

gaagtgatgc ccagaacact ggaaggctgc atctccgtga aggaccacag aacaagccag 1920

aaccacagca agtgcatcac cgagtgtgtg cggacagccc tgaacagaag aagcaagatc 1980

gaggcctctc ctatcggctc tatcgccctg agatccatgc ctaagagcag aaaccaggtg 2040

ctgttcagac ggaatctgac cccaagccag ctgagaactc tggcccctcc ttatatgctg 2100

ctgccccagt ctctgagcca gagcctgagc agaaagcaga tccctagcca gtccatgctg 2160

gtggcctcta gagcaggaca gtttcctgtg tggcggagaa ccagcagagg aatgagcctg 2220

cagtggaagt ccggcaatgc ctgcacacct ctgagatgcc ctagcagaag ccctgccaca 2280

ttcctgacag ctctgaacgg ctggcacaga tctggactga gagccgag aaagcgcaag 2340

cgcgagaaga gaagccagaa gtccaggcag aaccatctgc agctgaagtc ctgccggcgc 2400

aagatctcca actggtccaa gctgagaagc ccctggatct tcgtgtccaa gaagagactg 2460

cccaagctga ccaagtgtga aatctgtgtc aagaacaccg ccatcctggc tatcaccctg 2520

atcacccctc aggaccctca tctggaccct gtgtgtcacc ctcatagaag gcctggatgg 2580

aagggccacc acagagtggt gttttctcat caccctagcg gcagcaccaa gagccactgg 2640

cagcaaagag aacgcggagg cggcagacgg gaaaagaagg gctgtagaga gagatccctg 2700

ctgtactcca gaatgtgcct ggtgcagatc cccacacaca cctgggagcc cgattacttc 2760

2820

ctgaccagac ctgccattcc ttgctgtagc tgcacaagcc acagcaaccc ttggagtcag 2880

agcagacacc cacacagaag cccacctacc tcttgtcctc cgctgctgga agcctttcca 2940

ctgcctagag catccctgag agccaccgtg ccaagacata gctctgtgct ggcaaccgga 3000

3060

ccaggccagc atctgtggct gtctcataga ctgcggctga gactgccaag aactcagcgg 3120

cctcctagat ccatcctgag ccttagactg ccactgaggc cacagagagg acccagacct 3180

ctgcatagat ggctgcagca gaccacaggc tgtagccaga gaagataccc cagactgaaa 3240

accttcggcg tgccactgggg ctccattctg tgtctggctg gatctctgag caccatgatg 3300

3360

atcggaagcg ccagaccagg accttggaga acatctgcta tgcctagcgc catgggcgtc 3420

gcactgccta caagctgtga atccggaaga tctcctcctg ccaccggcgg aagaatgcct 3480

ggaagccaga gcattatgat gagctggatg cctcctctgg ctccttgtgc cgcttgtcct 3540

tgttctcccg ctcctacact gtgtcccgct catcctgcta gagcacctac agccgtgcct 3600

gcctttacac ctctgtctgc acatcctgtg cctgtgctgt ctggatgtca cctggccgtc 3660

agaacctcca tgctgacact gctgcctatg ctgaagctgg ccccactgca agtgaccacc 3720

acactgaagc tgatcctggt catgctggaa atcgtgaccg agagcactcg gagagagatg 3780

ccttctcagg ccacaccttc tccacctgga ccttggcgag ccgctctgggg caaagaagtg 3840

aagagatccc agagcaagct gcgcctgaga aacacaagcc ccatcgtgtt ctttctggcc 3900

ccatacttcc ccagacggat gaccagacgg accatgacaa ccaccaaaat gatgatgatg 3960

cggcggacca agcggagaag gcgcaaaagg cggagaatga caatgatgac aaggcggacc 4020

ctgctgacaa agaaaacctg tctgacccct agaaccagcg ccgctaccaa agctctgcca 4080

aggcagacaa tctcccagcg gaggcctatt cagacccctc ctggaacatc tctggccatc 4140

tggggcaacc agcagctgaa atggacccct cacatcctga agtccgccag cagaagggcc 4200

agacctacca gctgtagcac ctgtagaatg tggtggatcg ccaaaaagcg gatcaagctg 4260

cggcggaaca gaaccagact gacactgaca ggcacaagaa gcagcggcac cagagcttct 4320

ccatccgccg tttgcctgtg ttgctggacc tgtccttcca gaagcttccc agccgcttct 4380

ccaagcggat tgagagcacc tgttggagca caggcccctc cagaagttac actgagagct 4440

agctgtgcct ccagcgcctc taaggcttgt gctggacttt ggccttgggg agcacaagtt 4500

cggcaaccat ctttgcctgg accatgcgct tctctgctga gcacactgtc tcagccacct 4560

ccacaggctc catctcaagt gtggacagcc gcaacactga gatgtcctgc tgttccagct 4620

gctgcctgtc ctcctggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1144

<211> 4672

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 2B cloning cassette

<400> 1144

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaagaaag 120

atgcagagcc tgaatcaccc cagcagactg accaacatga gcagaacacc tccaagacgc 180

gtgtgcaacc ctcggagaaa gacctggaag atgcggaaga agtacgccca gaagcctagc 240

cagaagaacc acctgtacaa cagccggaag aaactgctcg aggacatgaa gcagaatggc 300

360

cctatccgga tgtgcgccct gtctcaccct agcttctaca tcagcagcac cgtgtgcttt 420

ctgagcaagc actgcatgac cgcctgcaca caaaagaaag gccacatgat cttcagccgc 480

gagaagatca tcaaccccac catcagctgc ccctttcaga gccccacaaa gcggctggaa 540

ctgagcctgg aaccttggat tccctacctg caccagcaga aaacccaagg cggcaagaag 600

agaagcaagt tccctggcac cagcctgcag tgctacctga acagatgcag cctgatggaa 660

cagatccctc acctgaagtc cgcctctcca cagatcatgc agagaaccag cgccgaccgg 720

ttccggatga agcggagaat gcagaacctg gacaatctgg gcgttccatg gccactgctg 780

cctggacctc tggaaaacac acagatcccc agaacaagcc ctctgtggaa gatcacaatg 840

gtgctgacat accccaactg cgtggtgcac gtgctgatgt tctggctgcc tatgagatct 900

ctgggcgagc cttggagagc tggctttcag tggggagagt ccccttggca agtgatggct 960

agcctgacag gcccctatcc tatcagcgag ggcctgacca tgaccaccgg cagaaacatc 1020

aacagcaact ccctgatgac caactgcctg ttcagcccca tcttcctgtg ccaccacgcc 1080

atcatgcacc ggaagatgac aagcccacac ttcagactgt tcagcagcaa gatcccacat 1140

cctcaggtgc catctgtggt ggccctgtgc aagttcagag ccacatttct gctcgccctg 1200

tgggagtgta gtctgcctca agccagactg tgcctgatcg tgtctagaac cctgctgctg 1260

gttcagtctg ccgctcctcc acatagactg ctgggccctt ctagatggtc cagaagccac 1320

ctggacttca gcccctactg gatgaagaaa gtgaagctgg ccaacggcct gtggttcaac 1380

ctgatcttcg tgctgttcaa ggccgaccag agcgagtcta gcctgtctag cggccggaac 1440

aagaaaacaa agctgtggtt ctccctcatc aacatccacc accgcaagaa tcctctgctg 1500

cccatgagaa tccccagcct gaatgcccag tggatgttcc tgagaatcct gcttctggcc 1560

catatgtacg gcttcagcac ctacggcacc cagaaaggcg agtgcgtttg tctgcagtgt 1620

ctgagcggca gcagcctgtt cattctgaag cagcccctgg acctgaagat ctccaagacc 1680

ttcatgctgc aagctcaccc acaggtgcca gctcttgctc ctcaagcttg gtggcctgct 1740

agaagaggcc ctctgacatc tgctccatcc gctcagcagt ctctgacagc tagccctcag 1800

attatgcagc ggatgagcgc cgtgaacttc gagacaatca tctaccctcc aagcacctgt 1860

agcctgcctt gtagaggcag aacactggaa agcgccggac tgtttccttg gctgcacacc 1920

cagtgtacac acccctgtac catgctgctg ccacaaggct ggtggtgtac aagaggcggc 1980

ggaaagcgga aaaagaaaat gtgcaaggtg tccggcggct gcaccgcctg tgttgagaga 2040

tgtccttgga agggcatctg ctggaagtgg ccagtgacag tggctgctca gtggtctagc 2100

gtgacctctc tggaatggac agcccaagag agaggctgca gcagctggct gatgaagcaa 2160

acatggatga agtcttggtc cctgcgggac cccagctaca gatccatcct ggaatacgtg 2220

tccaccagag tgctgtggat gcccacaagc accgtgcgga gaagaaacag cagaaagcag 2280

tctcaggtgc agctggccat tcaggtgctg ctcgtggaca agcacaagta ctttctgttc 2340

ggcagagccc gcaaccggaa catgaagaaa accatgagcc acctgatcgc ccatcaggcc 2400

ctgaagatga gcggcctgag gctgtttcat cctcggctgc tgacaagaaa gaagcccggc 2460

aagttcctgc ctagcagatt tccttggggc cctcctcctc tgtgtcatcc tgcttggctg 2520

agccctatct ccgcctgtcc aagactgctg ccatatcctt ttcacgtgct ggccctggaa 2580

gtgacctttc cacctggaac agccatgcct cagatgcaac tgcaggccct ggccatgtgg 2640

gagaaaggct gggataagcg gaagagaatg aagaccggcg ccatccaaga gaagggcgct 2700

attcaagaga aaggggccat gaccaagcgg atctgcgtgg tcaatttcga ggcctgcaga 2760

gagatggaaa gcctgagcgc ccctgagaag attaccctgt ttgaagccca tatggcccgg 2820

tgcacctcca tcaatgtgct gtgtgtcaga gcctctctga tcgagaagct gatgaaagaa 2880

aagcgcaaga tgaagcgcaa ccaggtggca tccccacaaa ttatgcagag gctgttcatt 2940

aagggctacc agaacaacca cttcaccttc catctgcaaa acttcgcctt cttcagcagc 3000

ttcggcaaca acaagtacat caatatcctg acgcggcgca agaaaagaga ggaccactgc 3060

ggactgctga tcctgatcca gcacggcggc atcttcacct ttcggttctg ggtcgaaaag 3120

ctgagctgga acatctgcaa gaacctgagc ctgtacctgg ataagttcgt gcagcggctg 3180

ctgtacgtgt acatcatcga gacaaacgtg ttcgtgctga acctgttcca cgtgccagtg 3240

aaccagatct tcatgatggc cttctacctg aagtacaccg agaaggcctg ggtcatggaa 3300

ctggaatggc tgatccaaca gcagtgttac cagcaagaga agaaaagccg gaaactgatg 3360

agcagctgcc gggccttcat ccacacacct agaagccctg gctgcagcgt gtacgccaat 3420

atgtgctggg ccttttggaa gcctcctagc cagggcatca gctcttgtct gatgacaggc 3480

tgttggggca gccctcctct gcacctgtgt cagcctctga ggcggtataa gaaaaggggc 3540

gtgatcgcct ggcggcggag aaagtggcag atccggcatg tgctgaaaaa cctgatcctg 3600

tttcatgtgc ggaacatcgt gtacggcatc caagtgatgc tgtgcggcaa gggactgaca 3660

caccctgtgc ctctgaaacg gaccctgcag aacaagagga acaagaacat caagcgggtc 3720

aaccagaaga aggcaacat caagaggaag tccatcctgc tgcacctcat tgtgctgaat 3780

agccccgaga gcaaccccaa gttcttcctg acctttagcc agctcgagaa gaaagctacc 3840

tggatgagaa gaacaggcgc acagctcctg ctacagcagc tcctcaggct 3900

cagactcctc attggagagc cgccacaaca agcagcatga gcagcagccc tagagccgag 3960

cctacatctt ctagctcttg ggccacctgt tggcccagat gctgtagctc cagctgttac 4020

tggtccctga gttcttggcc tcctgctggc gccgctgagg ctacaaacac cagaactaga 4080

agcagagagt cccagcgggg cagaatgctg acttggagaa gtagtctgtg gctgcaggggc 4140

accagatgtt ttacaggcgt gcggacaagc agcggaagct gtggcagagc ttgtagctgc 4200

ggaagaggct gtggaacccc tagaggcgat tggaagagac accctcctcg gaggaatagc 4260

gtgatcgtgc acaagatcgt gctggtgttt ctgaagtccc tgtccagcta caactgcgag 4320

agaaggatca gcggcatcca gaagagcc atcgagtttg ccgtgctgaa actgctgaat 4380

atggacctgc actggaactc cgcctacctg ggcagaactc tgctggtcac ctgtatcctg 4440

tatcaccggt ggatcgtgac ctccatgtgc cagtctcaga gatggctgac cagcaccaca 4500

agcagatcca gcgctctgat gtggacccgg cagatcaatc ggaggaagct gcagagaaag 4560

aagaggcacc caagctggcc ttggaccaga tgccttagaa tgaggcctcc tagaagcggc 4620

tacccctacg atgtccctga ctatgcgtcc taaaggcgcg cctagcggcc gc 4672

<210> 1145

<211> 4687

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 3B cloning cassette

<400> 1145

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaggagct 120

gctgctgcct cttgtcagtc tcctccttct cctcgggaaa ccacacctag ctccgacgcc 180

accagacgga caagaccaat tgccgctcag agcaagaaaa ccatgacagc cgccggactg 240

acccctaaga acaagaagcg gagaaagctg cggcccaaga tgatccggaa gatccagaag 300

agaaggcgga ccgccaaccg gcggaatagc agaagggcta cccctacaag caagggcagc 360

ctgagaagaa agtacctgag agtgcgggga ccctcctctgc acacccggaa catcagaaag 420

ctgatctgga ccaagcagcg gagcttcctg tctctgttcc ccaagctgag agccaccgag 480

cagatgacca atgtgggctg ctctatccct cacgtgccca aagccacagc cgctcactct 540

gtgacagtga aactgcagct gcccctgacc ttcagcaccc tgctgtggga tagcagacac 600

accatgagcc tgagcaacat cctgatgaag ttccggctgc agtctagccc attcctggcc 660

agatcctgga acaccatgca gtggaacaac aagaaaatgg ccatgaccgt gatcgctggc 720

gcccaccact gtcagtacaa gctgacctac acccaaagtgt ggaagatgaa ctggaagaaa 780

atgatgatcg gcagccagca tctgctgttc agccctctgg atagcagtcc cctgcagctc 840

ctgctgcacc ctcacggcaa gagatgcaac ccttgctgca gactgacctg gatgagccag 900

aacaccagaa gcaccaacct ggaaaagaac cccagcctga agaagcagag ccagcggaga 960

ggaaagctga gcgcctttag cggcagaatc ctgcaccagc cagaaatggc cgccatgaga 1020

ctgatgccca agttcctgaa ctccaccaac agctggtgga ccctgaccac actgagcttc 1080

1140 gtgctgatcg aggaagccag acagcccagc agacctaccg

ctgatggccc ctgtgggcag cgtgatgagc tgttctctgc tgacccagcc ttctccagcc 1200

agcgcctgta caatgcctcc actgctgcat ccacagtggg cccctcatag tgctggactt 1260

tctccaggcg cttgcagacc tgcctgcacc tgtatcagca tgaccaccat cagcaggggcc 1320

acatggggcc cagtgtacta cgtgtgcaac cggatgaacg tgtccagcgt gctgaagccc 1380

ttcctgttca ccgtgcctat cgaggacatg agagagtgca ccctgggcag aaacagaatg 1440

aatgtgtcct ctgtgctgaa accctgtctg atccctgtgc caatcagcga gaagaagagg 1500

aagctgccca acatcgagat cacccctcag cggtctaagg ccatgctgca gaagcagtgc 1560

tgctacaacc tcgtgatcaa gcaccctctg atcttcagaa tcaacctgcg gcggcagggc 1620

aagaccaaag atggcgttgt gcagagcctg ggcttcctca accagcagat catcctgcag 1680

aaagagttct tcatctgcct gctcatgttc atcaagcggt ggaagcctgc cgccaccaag 1740

agcagaaaga agggccacac caagaacaga cggaaggcca agagaaagcc ccagatctac 1800

ctgttcttcc tgaccgtgtg cggctttcac ctgaaaaaga ccagcgccaa gtggctgaag 1860

aagtccacca agtccagatc caacggcatg gaagcttgcg gcacacaccc cactcctaga 1920

ctgagctgcc tgagagttag atgcggcggc tggctgtgtg gctggacacc tagaggtaga 1980

aagtccggcg tgatgaagtg ctgggatctg tgccccgtgc acaagaagag aggcctgttc 2040

agactggatt ttcaccccag aagcagctcc ggcaagggaa agaacagccc ctgctgccaa 2100

aagaaactgc gggtgcagaa ccagggccat ctgctgatga ttctgctgca caatgaccgg 2160

gtgttcctcg tgaagatcgg cgagaagctg aagcactgtt tcaaggcctg gggctccaga 2220

cggaaccggc ggtacagaaa gatctacggc gagctgaaga tgtccctgca ggccttcctg 2280

atccacctga gaaaggtgga cacccaccac ctccaggtgg tcaccaaaac agtgaccgcc 2340

acaacagccg acggccagag aatgcggaga ctgaagtgca aggcctgccg gctgagatgg 2400

gccagacctg aacctgctgc tcaggcccct cctaagagcc actttccacc tcatcctagc 2460

ggccccagca gaacaagtag cctgcagctg agaagcctgt tctttagcca ctgcctgtgc 2520

agccagaaga aggaccctgc cggcaagctg aaaaagctgc tgaagaaaag cagccagctg 2580

cggagaagct ggcgcaatgt gtccagaatc agcacccctc ctccactgat cctgtggcct 2640

caagctgctc ctgtgtccgt gatcaccatg aagctgtggg agaagcactt caagggcctg 2700

aattggaacc tggtcgtgtg gattctgaac ctgaagatga tgacctacat ggaaatgcac 2760

tacggccatc aggccccaca ggtggcaatg ctgcctaagc tgctgtgcag acagatgctg 2820

cccactctga gcacactgca gcagagacat cctcaggggcc cttgtcctcc tcctgtgaca 2880

cctctgagag tccagtctgt cctgctgctg ggagtgcctc agacagccag aggacctaga 2940

agaaaggtcc tgatggtgct gtggctgccc gccaatcagt ctctgcagaa aagccaggtt 3000

ttccagtggg tgctgcggac agaggtggcc cctagatgtc ctagatctgc cgtggacatc 3060

agccccggac ctacatctcc tagcctggcc tcttgtgcct ctgtggccat tggaaagctg 3120

gcactgcttg gcgcctctgc tccttgtggt gctgctggcg ctagagaggc cggaagattt 3180

gctgtgctgg gcaccatggt catgaagctt tgtttcttct acctgctgta ccggctgcgc 3240

atcccaagcc tgaggaagta cagacagcac ctgaaccgga agctgcacct gacaagatcc 3300

tgcctgatcg atctgaccat gatgatgtcc cagaagctct ggaagaacca gcggaagaga 3360

atccctctgc catctccg gcaacatctg ctgccacagt accctctgca cccacctcca 3420

ccttgtctgc tgttgctgca ccatagacac cacctggcct ttctcgagat ggcctgtagc 3480

tctcagcaca cccacagcat caagatctgg atcagcttca gccatgaagt ggaccctcca 3540

ggcagcgtgt ggatactgcc ttgcgatctg cctcggatgc tgccaatctc ttcacccctg 3600

cacagacccc agatgccttt ctggacctgc acctttcctg ccacaacctg gggcacatgg 3660

gagacacctt ctacatgggg ctggcggact ttcagaggcg ctagcagcag cacatcccgg 3720

gacagcagat ccttcttcac ctctgctcac cctagcaggc cctgcagaat gagactgtac 3780

cagtgcaaga agaagctcct cctgaaactg ctgaccacca ccacaagcag ccccagaaga 3840

tcccctgctc tgagagcaag aggcggcacc acaatcggcg aagtgaccag ctgtaccaac 3900

ctgagcgtgc ccatgatgct gaccatcctg atttggaagc gggtgttcat cctcctgctg 3960

agcgataaga agggcgccaa gaagatcacc ttcctgccta ttcctccaca cctgagacac 4020

cctctgctgc tgttcgagaa gaaacggcct gagtggctga gcatggacct ggacggaatg 4080

ctgacagtga tcggccaggg actcgtgggc atgaccccta gagatctggg aacagtggca 4140

ggcgccaccg ctcagattca gcgacctatc caggtgcaga ctggcggcag actcagaaga 4200

aacctgcaga gaaacctgaa ggtgctccag gtgtccgtgt acagcggcaa cctgctgtcc 4260

tttagcagac ggggcatgaa gtctagcgtg cggctgaaaa accgcgagtg ggagaaacgg 4320

aagcagattc tcggaggcgg cggaaagtac aaagagtact tcctgaagcg catcctcatc 4380

cggaaggcta tgacagtgct ggccggcgac aagaaaggac tgggcagatt catgagatgc 4440

gtgcagagcg agacaaaggc cgtgtctctg caactgcctc tgggaagaaa catggaacac 4500

cggttgacca tggacagcgc cctgagcctg acaaagagag agggacccga gacactcaaa 4560

aagaaatacc tggtccagca ccagatgaag tgtctgaccg ccgtggattg tctgcaggct 4620

ttttgggaca tgggctaccc ctacgatgtc cctgactatg cgtcctaaag gcgcgcctag 4680

cggccgc 4687

<210> 1146

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 4B cloning cassette

<400> 1146

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaatccag 120

aagaagaagc agaagtccaa cctggacctg cagcaccagc gcagacgcaa aaagaagaag 180

gacaaggtcg agatccggaa tcggagcccc aaggccaata gcacagagtg tcagggcgag 240

cacagcagag agcagaacct gctgaatctg gtgcagctga accctcacag ccctcaccac 300

agaaaggacg aagaggacca ccagaagcgg caccaccacc acaatcaaaa gaaaatgagc 360

gtgatggcca agaagtccca gcagcctctc ggacaggcct ttggctctct gtgtcagctg 420

gtgtccgccg atttcagcgc ccctcaagtg accagactgc ggagcctgaa ccatctgctg 480

atcaacgagg gcaacaacca ctggcctaga gtggaaatgc ctaccggttg gctgctcgtg 540

ggctacaaca ccagaaacac agcccagaat cctcggctgc agcagcacct gaaacactgg 600

cagcagtgca ccagagccag atggtgccaa gagccttgtc agaccctgac cttcttccat 660

ttcttcttct ttttcaaaac ggtgtccctg tgctgccccg gctggtctaa aggacaacct 720

cctccacctg gcctgaagcc ttctagcctg gcttctcagg tggccagaac cacaggcgtg 780

taccagcacg tgtggctgat ctgcttcgtg ttcttcgtgt gcgtggaaac cggcttctgt 840

cacgtggccc aagtgggact cgagagactg gtgtctctga gcggcatcac cgacgtgaac 900

cattgggccc agcctaagcc atctcctagc aaaggcaccg gcatgaacag acccatcggc 960

ctgagagttg gcaaaggcgt gtgggccaga ctgagagcct ccgtgtcttt ctggaccctg 1020

ctgcctacaa caggcgtgcg gacaagagga agccctctga gactgacact gtggcctcca 1080

cggaacatcc tgaacagcct gcctagcagc atggaaatcg ccgtgctgca agagaaccgc 1140

aagaccagaa aggccgccag agagaagcac agaaagaccg acaacctgca cctgtgctac 1200

taccacagcc agagcaaccg gaacaagagc cggcagatgg aaagcctggg catgaagctg 1260

cagagccctc tgtgggattg ctccaccaca acaggcacac accacggctg tctgtgggag 1320

atccctagcg ttacactgct ggcctgcaga gtgaacgccg tggaagaact gcacagcagg 1380

1440 cggatgtctg tgcggatcgt gaagaactgg gtgtcctgct

gtgggcctgt ctacttggag cgccctggac gtggtcatca acctgaagcg gctgcagaag 1500

ttcagccaga agtctcagca ccactgcgcc ctgagaaaga tcgagtgggc cacaaagctg 1560

agcctcggcg gaaaagctgg accccaggct caagagaagg ccaacgccaa gaagatgccc 1620

ttccagcggt ggatcttcag caatcagctg accgtgcaga tgctgtgcct gcctattgaa 1680

gccctgactg ccctgttcac ctgtcggaca gccagcatca gcgtgaacat cagcaccctg 1740

atgagcatcc tggaagcccc tctgtttttt cacggcgacc tgtgcgagga aaagaagaac 1800

ggccggatca gcagccaggc tcctctcaga ctttggtctt ggtgcggcac aagccagacc 1860

tactttggca gcaccgccaa gaaaagcccc accagctttt gctgcgagtg gggaggacag 1920

gctcaccggc agagaaatac cggaatggaa atcctgctgt ggattctgct caagatggaa 1980

attcagatct tcaagatttg cctgggcgag atgcagctgt gcacctacac accccacatc 2040

tgcagacaca cctgtagagg cggacctagc gccagcgata gatgctgtag ctgtagccct 2100

agcggcttca gcgccggaag aggcagatgt ccagttcagg gatgtctgcg gcctcacaga 2160

gtgcagctgc tgagaagatg gggccctgga tctcctgctg gccagagact gtctaagggc 2220

aaagggccca 2280

tttccaccac ctgatcctcc atggcctgtg accgctgtga cagtgatggc tggctctgtg 2340

cctagcgctc agtctgtgga tgctctggaa agccctggac ctctggcact ggaaggacct 2400

agcagcccca gaaacctgct ctggcgggaa atgagcatct tcctgcctgg catcttcacc 2460

tctcggattc tgaacctgca agtgctgaag aagatcctgc ggagctttat gaagctgtac 2520

cactctctgg tcatgtgcct gcggctgcgg accaagctgg aaaaagctct gagcgctctg 2580

ttcatctggc cccagcacag ctacaagcag aggcctcctc tgcagcctgt gcaggtcgtg 2640

ctcagactgg gaaacctgcc tccattcttc agaggcagcg aggtgccctt tcctagacct 2700

tgccggacca agatgctgaa ccagaagtgt ctggcccctt tcatcacaca cccattcgcc 2760

tctcagcccc tgggacaaga gatgcccaac aacacccaga tcagactgct gaagcccctg 2820

ctgacccctg acaccagatg gatcttctcc aaccagctgc agacagtcca gatgctctgt 2880

ctgccacctc tgaagcaggg cgagagaatc atttggcccc tcggcggctt tcagatcccc 2940

aacttctttt ggagcctcgg aatcggccac actggcgcct ggtggcatac tggaaggcgg 3000

agaagagaat ggggcggctc caccgtgtct agaagcccac tgtggatcag ctccatgatc 3060

tacctgagag gcatggcctt cgccagcgac agctccatca gaaccaccag agtgtcctgg 3120

tgcagaagaat gcggcgccaa atctgccgct gcctccagcc tcaagaagaa accccgggac 3180

atctgtcgga tcagcgactc taagaagatg acccagaagc gcgagagcac cctgggcaat 3240

ttctggatga agctcctcca gctgccagac aagaagaaga agtctaccga aaagaccaag 3300

atcatcaaga agatgaagaa acggatcatg gtccacatgt gcagctccct ggctctgcca 3360

acaggcctga catctctgat cctgccaagc agcgatctgg ccgtgctgat ctctagctcc 3420

accagccact ttctgatgcg gagcccagtg ctgcctagct ccagactgac ttgtgcctct 3480

ccacagctgc ccagaatgtg gacctggtcc agctggctga agtggctgaa aaactggaag 3540

cggttcaagc ttaaaaagaa gtacatcctg gccatgacgc tgaccctgaa cgggcatcca 3600

agaaccgcct ctatcaccct gcctaccacc agcctgaagg gctctagaac cagctgttgc 3660

cccagatctc accccagact gcctaggaca aagtggcctc tgcagtgcct gctgcacagc 3720

ctgggaacaa ctggaacact cgtgtgggaa aggggcgtgc tgcagatgag aagtctggaa 3780

gctcactgcc ctctgcagac cttccacctc caccactgta gaactcagcc cagctcttgc 3840

ccctcctgcat ggattctgta ccaaggacag tggagcggag ccgtcgtggg acttagacct 3900

gtggctcacc atctgccaag acctattcag ggcctgcact gcccacaggc tccacatcaa 3960

ccagctctgt cttggggcaa gtggcccgtg ggaatgacag gacctgccag atgttgccgg 4020

tggcctagac gggaactcaa aaagaatctg ctgctcccct gccggtgcag acgcgagtat 4080

agagtgacaa tgggccaagc cttcggcagc tttagcgccg actttagcgc tccccaggtg 4140

ttgaagaaga atcggaagca gaacaagatt atctgcaagg gccaccgcat cacaagcctg 4200

atcctgagct ttcagcgcgt gaaacttaag aagaaagtca acgcctgctc tcccgcctgc 4260

acctctaaag cctggactcc cagaaagctc gtggggagag ccgtcagaag aaagggcatc 4320

atctgtatca agaatatgtt cctgcagcgg aagggtttcc tgaacctgag ccacgtggaa 4380

aagttcgtgc ctccttctcc tcctctggct ctcggcccta gaatgcagct ctgtacccag 4440

ctggcccggt tcttccctat cacacctcct gtgtggcaca tcctgggacc tcagaggcac 4500

accccttgtc aaaagaaggt gccctccgct agcccatggg gccttagacc atctccttgc 4560

ctgagctggt cctccagaat tgtggcccct ggacacgccc tgagctgtcg gactagaaga 4620

agatggtcca gcaagggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1147

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> Cloning cassette hINV 1A

<400> 1147

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgaccgaga tccagctgac catgaacgac agcaagcaca agctggaaag ccccgctctg 780

aaacaggtgt caccagcctc tccacctact cagcagcctc agacacctca ggattctaga 840

caggtgctgg ccctggccaa gaaaaccctg acaaatgcct ccagagccgg acagtttcct 900

gtgtggcgga gaacaagcag aggcatgagc ctgcagtgga agtccggcaa tgcctgcaca 960

cctctgagat gccctagcag aagccctgcc acattcctga cagccctgaa tggctggcac 1020

agatctggcc tgagagccca gatgcctagc tcttggtcta gcagcggcag aacactgaag 1080

ctggcccctc tgcaagtgac caccactctg aagctgatcc tggtcatgct ggaaatcgtg 1140

accgagcgct ccctgctgta ctccagaatg tgtctggtgc agatccccac acacacctgg 1200

gagcccgatt acttcgtgtc cctgcctatg atgttcagct gggccacaca gtcttttccc 1260

gtgggcgccg tgtttctgac cagacctgcc attccttgct gcagctgtac cagccacagc 1320

aacccttgga gccagagcaa tcagcagctg aagtggaccc ctcacatcct gaagtccgcc 1380

agcttcgaca agaaaggctt cgtgatcctg agccggcgga gcaagattga ggcctctcct 1440

atcggctcta tcgccagctc tcctggacat cctctgggac ctacactggc catcagattc 1500

atcagcgccc ctcagcctag cagctctaca gtgacagctg cccctagcgg ccagtacttt 1560

atcgccaaga agcggatcaa gctgcggcgg aacaatatgg gcggcaacag atccatctgc 1620

ctgaccagaa agcgcaagag agagaagcgg agccagaagt ccaggcagaa ccatctgcag 1680

ctgaaaagct gcagacggaa gatcagcaac tggtccaagc tgagaagccc ctggatcttc 1740

accaagtgcg agatctgcgt gaagaacacc gccatcctgg ctatcaccct gatcacccct 1800

caggaccctc atctggaccc tgtgtgtcac cctcatagaa ggcctggatg gaagggccac 1860

catagagtgg tgttctctca ccatcctagc ggcagcacaa agagccactg gcagcaaaga 1920

gaacgcggag gcggcagacg cgagaagaag ggatgtagag aacagcggaa ggtggtcaag 1980

aacccctctc agcccgtgcc tctgagaaac gatggcatca ccaacggcaa cgactccacc 2040

agaagctgct ccatcagcct gaagaaaagc ctggaaaaga tgcccctgat gcggagagcc 2100

agacctacca gctgtagcac ctgtagaatg tggtggaagg gcaagaaacg gcggaaagaa 2160

atcgtgccta tgagcctggc ctgcagcatc accgtgtctt ttgtgggaca gatcagcacc 2220

ctggtgctgg atgtgtctag catcagccat tggcggctgc cctgtaaagt gaccgagagc 2280

agctccagct tcttctacac cagcgacagg acccctctga gcatcagcag acccaaccagc 2340

agagctagac agagctgttg tctgccggga ctgacacacc ctgctcacca acctctgggc 2400

agcatgagca ctagaagaga gatgccaagc caggccacac cttctccacc tggaccttgg 2460

agagccgctc tgggcaaaga agtgaagaga tcccagagca agctgcgcct gagaaacaca 2520

agccctgggg agaagaacga tctccagctg ttcgtgatga gcgacaccac ctacaagatc 2580

tactggacag tgatcctgct gaacccctgc ggcaacctgc acctgaaaac aacatcactg 2640

cagcggccac ctcggagcat cctgtctctg aatggaatct gggaagtgat gccccggaca 2700

ctggaaggct gcatcagcgt gaaggatcac agaaccagcc agaaccacag caagtgcatc 2760

accgaatgtg tgcggactgc cctgaacgtg tccaagaaga gactgcctaa gctgatgaag 2820

aagaaggaca gcaccatctt catcaacaaa gtgcgccaga acttcaagcg caacttcaag 2880

aagaagaaac ccctcacttt cagccccagc agacggatga ctcggagaac catgacaacc 2940

accaaaatga tgatgatgcg gcggaccaag cggagaaggc gcaaaaggcg gagaatgacc 3000

atgatgacca gacggaccct gctgacaaag aaaacctgtc tgacccctag aaccagcgcc 3060

gccacaaaag ctctgcctag acagaccatc agccagcggga ggcctattca gaccccacct 3120

ggaacatctc tggccatctg gggcagaaca agactgaccc tgacaggcac cagatccagc 3180

ggaacaagag cctctccttc cgccgtgtgc ctgtgttgtt ggacctgtcc aagcagaagc 3240

ttcccagccg cttctcctag tggactgaga gcacctgttg gagcccaggc tcctcctgaa 3300

gtgacactga gagcctcttg tgcctctagc gcctctaaag cctgtgctgg actttggcct 3360

3420

gctgaagctt ctacaggcgc atggacagct gaaggcgccg ctggaacaag atgtagagcc 3480

tctagagccc cttgccgcca acttcctgct agctccccta ccaagtgtga aatttgtgtg 3540

aagcgcagac ggcggagaaa gagaagcagc cagtctagaa gccccgtgcc taaaaagcgc 3600

gagaggatga tgctgcctgt gcgggccaga acagctagat ctgcacttcc tcagggcaga 3660

gcacctctgg aagggatgct gcttcctaga gcactgccta gcctgctgag cagaatgcct 3720

gccagaagag agtcccggtt ttggctggct ctgcaggcac ttggactcag acctccacat 3780

ctgcctagct gtcatcctct gcctcaactg ttgggccctc cacctcctca tcaggcccca 3840

atgacactga aggccctggt taagacctgg acacagttcc gcaactaccc cagagccgac 3900

aagaagaatg tccggtctac cgccttcttc ctgctggaca tcatcctggg cgattgccca 3960

agaacaatgg ccggaccttc tgcctctctg catcctgcat ctggcagagc ctccagaaag 4020

acctggcaga gaaggctgag agcttgtaga gcaggcggac ctgctggatg ggctagacag 4080

gcctatccta gactgaaaac cttcggcgtg cccctgggct ctattctgtg tctggccggc 4140

agcctgtcca caatgatgat cattagcacc accagacaag agtgcggcag gcgggcttct 4200

gtgccttgta gacctatgat cggaagcgcc agaccaggac cttggcggac atctgctatg 4260

cctagtgcca tgggagtcgc cctgcctacc tcttgtgaaa gcggaagatc tcctcctgcc 4320

accggcggaa gaatgccagg atctcagtct atcatgatga gctggatgcc tccactggct 4380

ccatgtgccg cttgtccatg ttctcccgct cctacactgt gcccagcaca tcctgctaga 4440

gcaccaacag ccgtgcctgc ctttacacct ctgtctgctc atcctgtgcc agtgctgtct 4500

ggatgtcacc tggccgtcag aacctccatg ctgacactgc tgcccatgga agccaaaaag 4560

agcatgaagc ggtccaacgc taagaaaatg gaagatatga tctggggagag ccggctgcca 4620

ctgaggccac aaagaggacc tagacctctg cacagatggc tgcagcagac aaccggctgt 4680

agccagaggc ggatcgtgtt ctttctggcc ccatacttcc ctcggcaccc tcacagatct 4740

ccaccaacat cttgcccacc tctgctggaa gctttcccac tgccaagagc ttctctgagg 4800

gccacagtgc caagacatag ctctgttctc gccaccggtc tgcacctcca cagacagatt 4860

tctctgcccc tgagaggatt cctcctgcac caaccactgc caggacagca tctgtggctg 4920

tctcacagac tgaggctgag actgcccagg acacagccat ctttgcctgg accatgtgca 4980

agcctgctgt ccacactgtc tcagccacca cctcaggctc catctcaagt gtggactgcc 5040

gctacactga gatgtcctgc tgttcctgca gctgcctgtc ctcctgctct gagatccatg 5100

cctaagagcc ggaaccaggt gctgttcaga cggaatctga ccccaagcca gctgagaaca 5160

ctggccccac cttatatgct gctgccccag tctctgtccc agagcctgag tagaaagcag 5220

atccctagcc agtccatgct cgtgggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1148

<211> 5281

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 2A cloning cassette

<400> 1148

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgagaaaga tgcagagcct gaatcacccc agcagactga ccaatggctc tcctccactg 780

cacctgtgcc agcctctgag aatgagcaga acccctccta gaagagtgtg cgccgctcct 840

ccacatagac tgctgggccc ttccagatgg aaccctcggga gaaagacctg gaagatgcgg 900

aagaagtacg cccagaagcc tagccagaag aaccacctgt acggcggcaa gaagcgctct 960

aagttcagca gaagccacct ggacttcagc ccctactgga tgaagaaagt gaaggtgctg 1020

ttcaaggccg accagagcga gtctagcctg agcagcggaa gatgcagcct gatggaacag 1080

atccctcacc tgaagtctcg gcggaagtgg cagatcagac acgtgctgaa gaacctgtgg 1140

ctgcccatga gaagcctgg agaaccttgg agagccggct ttcagtgggg agagtctcct 1200

tggcaagtga tggcctctct gaccggcaga gccacatttc tgctggccct ttgggaatgt 1260

agcctgcctc aggcaagact gtgcctgatc gtgtctagaa ccctgctgct ggtgcagagc 1320

cggaacaaga aaacaaagct gtggttcagc ctgatcaaca tccaccaccg gaagaaccca 1380

ctgctgccta tgagatggtg gtgtaccaga ggcggcggaa agcggaaaaa gaaaatgtgc 1440

aaggtgtccg gcggctgcac cgcctgtgtt gagagatgtc cttggaaggg catctgctgg 1500

aagtggcctg tgacagtggc cgccattaga atgtgtgccc tgtctcaccc tagcttctac 1560

atcagcagca ccgtgtgctt tctgagcaag cactgcatga ccgcctgcac cagaaagaga 1620

atgaagaccg gcgccatcca agagaagggc gctattcaag agaaaggggc catgaccaag 1680

cggatctgcg tggtcaattt cgaggcctgc agagagatgg aaagcctgag cgcccctgag 1740

aagatcaccc tgtttgaagc ccacatggcc cggtgcacct ccatcaatgt gctgtgtgtg 1800

cgggccagcc tgattgagaa gctgatgaaa gaaaagcgca agatgaagcg gaaccaggtg 1860

gcctctccac agatcatgca gcggctgttc atcaagggct accagaacaa ccacttcacc 1920

ttccatctgc aaaacttcgc cttcttcagc tccttcggca acaacaagta catcaatatc 1980

ctgaccatcc tgttccacgt gcggaacatc gtgtacggca tccaagtgat gctgtgcgct 2040

agccctcaga ttatgcagag aaccagcgcc gaccggttcc ggatgaagag aagaatgcag 2100

aatctggaca acctgggcgt gccatggcct ctgttgcctg gaatcccatc tctgaacgcc 2160

cagtggatgt tcctgagaat cgcatccccg caaatcatgc agaggatgag cgccgtgaac 2220

ttcgagacaa tccgggccag aaaccggaac atgaagaaaa ccatgagcca cctgatcgcc 2280

catcaggccc tgaagatgag cggcctgaga ctgtttcacc ccagactgct gacacggaag 2340

aagcccggaa aactgctgct cgcccatatg tacggcttca gcacctacgg cacccagaaa 2400

ggcgagtgcg tttgtctgca gtgtctgagc ggcagcagcc tgttcattct gaagcagccc 2460

ctggacctga agatcagcaa gaccttcatg ggccacagag agggcagcga gtctgataat 2520

ctgggcctgc tgtatagctt ccctccacct tatcctatca gcgaggggcct gaccatgacc 2580

accggcagaa acatcaacag caactccctg atgaccaact gcctgttcag ccccatcttc 2640

ctgtgccacc acgccatcat gcacagaaag atgacaagcc ctcacttcag gctgttcagc 2700

agcaagatcc cacatcctca ggtgccatct gtggtggccc tgtgcaagtt cggggccttc 2760

atccacacac ctagaagccc tggctgcagc gtgtacgcca atatgtgctg gcccaagttc 2820

ttcctgacct tcagccagca gtggtccagc gtgacatctc tggaatggac agcccaagag 2880

agaggctgca gcagctggct gatgaagcag acatggatga agtcttggag cctgcgggac 2940

cccagctaca gatccatcct ggaatacgtg tccaccagag tgctgtggat gcctacctct 3000

accgtgctgc aggctcatcc acaagttcca gctctggctc ctcaagcctg gtggcctgct 3060

agaagaggac ctctgacatc tgccccatcc gctcagcagt ccctgaccag agagaagatt 3120

atcaacccca ccatcagctg cccctttcag agccccacca agagactgat ctaccctcct 3180

agcacctgtt ctctgccctg cagaggcttc ctgcctagca gatttccttg gggcccacct 3240

cctctgaaca gcgtgatcgt gcacaagatc gtgctggtgt tcctgaagtc cctgtccagc 3300

tacaactgcg agcggagaat cagcggcatc cagaagag ccatcgagtt cgccgtgctg 3360

aaactgctga atatggacct gcctctcgag aacacacaga tccccagaac aagcccactg 3420

tggaagatca caatggtgct gacatacccc aactgcgtgg tgcatgtgct gatgtttggc 3480

gccctgagag ccacagctcc tgctacagct gcacctcagg ctcagacccc tcattggaga 3540

gctgccacaa caagcagcat gagcagcagc cctagagccg agcctacatc ttctagctct 3600

tgggccacct gttggcccag atgctgctcc agctcttgtt actggtcact gtctagctgg 3660

cctccagctg gcgccgctga agctacaaat accagaacca gaagccgcga gagccagagg 3720

ggtagaatgc tgacttggag aagctccctg tggttgcagg gcaccagatg ttttacaggc 3780

gtgcggacca gctccagata caagaaaaga ggcgtgatcg cctggcggag acagatcaat 3840

agacggaagc tgcagagaaa gaagcactgg aacagcgcct acctgggcag aacactgctg 3900

3960 gtcacctgta tcctgtatca ccggtggatc

ctgaccagca ccacaagtag atctagcgcc ctgatgtgga ccgagctgag cctggaacca 4020

tggattccct acctgcatca gcagaaaacc cagcggacac tggaatccgc cggactgttt 4080

ccttggctgc acacccagtg ccaagagaag aaaagcagaa agctgatgtc cagctgcaca 4140

cacccctgca caatgctgct gccacaggga tgtcatcctg cctggctgag ccctatctct 4200

gcctgtccaa gactgctgcc atatcctttt cacgtgctgg ctctggaagt gacctttcct 4260

cctggaaccg ccatgcctca gatgcagttg caggctctgg ctatgtggga gaaaggctgg 4320

gataagcctg gcaccagcct gcagtgctac ctgaacagaa gaagaaacag ccggaagcag 4380

agccaggtgc aactggccat tcaggtgctg ctcgtggaca agcacaagta ctttctgttc 4440

ggccaaaaga aaggccacat gatctttagc ctggccaacg gcctgtggtt taacctgatt 4500

ttcaactccc ggaagaaact gctcgaggac atgaagcaaa atggcttcac cggcaaaggc 4560

ctgacacacc ccgtgcctct gaagcggact ctgcagaaca aaagaaacaa gaatatcaaa 4620

cgcgtcaacc agaagaaggg caacatcaag cggaagtcca tcctgctgca cctgattgtg 4680

ctgaatagcc ccgagagcaa cctgatcctg atccagcatg gcggcatctt cacctttcgg 4740

ttctgggtcg agaagctgtc ctggaacatc tgcaagaacc tgagcctgta cctggacaag 4800

ttcgtgcaga gactgctgta cgtgtacatc atcgagacaa acgtgttcgt gctgaacctg 4860

tttcatgtgc ccgtgaacca gatcttcatg atggccttct acctgaagta caccgagaag 4920

gcctgggtca tggaactgga atggctgatc caacagcagt gctatcaggg cagctgcggc 4980

agagcttgtt cttgtggaag aggctgtggc acccctagag gcgattggaa gagacatcct 5040

cctcggag ccttctggaa gcctcctagc cagggcatct ctagctgtct gatgaccggc 5100

tgttggctcg agaagaaagc tacctggatg cggcggaccc gccggaagaa gagggaagat 5160

cattgcggac tgagacaccc cagctggcct tggacaagat gtctgaggat gaggcctcca 5220

agaagcggct acccctacga tgtccctgac tatgcgtcct aaaggcgcgc ctagcggccg 5280

c 5281

<210> 1149

<211> 5296

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 3A cloning cassette

<400> 1149

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgggagctg ctgctgcctc ttgtcagtct cctccttctc ctcgggaaac cacacctagc 780

tccgacgcca ccagacggac aagacctatt gccgctcaga gcaagaaaac catgacagcc 840

gccggactga cccctatgat cgggaagatc cagaagcggc ggagaaccgc caaccggtgt 900

ctgagagtta gatgtggcgg ctggctgtgt ggctggacac ctagaggaca gaacaccaga 960

agcaccaacc tggaaaagaa ccccagcctg aagaagcaga gccagcggag aggaaagctg 1020

agcagcctgt tcttcagcca ctgcctgtgc cggaacagca gaagggccac acctacatct 1080

aagggcagcc tgcggcggaa gtacctgaga gtgcctattc ctccacacct gagacaccct 1140

ctgctgctga tggacctgga cggaatgctg accgtgatcg gacagggact cgtgggcatg 1200

acccctagag atctgggaac agtggctggc gccaccgctc agattcagag gcctattcag 1260

gtgcagaccg gcggcagact tacacctcct cctctgattc tgtggcctca ggccgctcct 1320

gttgatcctc ctggctctgt gtggattctg ccctgcaaga acaagaagcg gagaaagctg 1380

cggcccaagc ggctggactt tcaccctaga tcttctagcg gcgtgtccgt gatcaccatg 1440

aagctgtggg agaagcactt caagggcctg aactggaacc tggtcgtctg gattctgaac 1500

ctgaagatga tgaacatcga gatcacccct cagcggagca aggccagaaa gtccggcgtg 1560

atgaagtgct gggatctgtg ccccagcgag aagaagagga agctgcctct gcctctgacc 1620

ttcagcaccc tgctgtggga ctctagacat agaggcgcca gctccagcac cagcagagac 1680

tccagaagct ttttcaccag cgctcacccc agcagaccct gcagaatgag actgtaccag 1740

ttcctgatcc acctgagaaa ggtggacacc caccatctgc aggtcgtgac caagaccgtg 1800

acagccacaa cagctgacgg ccagagaatg cggagactga agtgcaaggc ctgccggctg 1860

agatgggcta gaccctgaacc tgctgctcag gcccctccaa agagccactt tccacctcat 1920

cctagcggcc ctagcagaac cagttctctg cagctgcgga gcatccctca cgtgccaaaa 1980

gccacagccg ctcacagcgt gacagtgaag ctgcagaagg gaaagaacag cccctgctgc 2040

caaaagaaac tgcgggtgca gaatcagggc catctgctga tgatcctgct gcacaatacc 2100

tacatggaaa tgcactacgg ccatcaggcc ccacaggtgg ccatgctgcc taagctgctg 2160

tgcagacaga tgctgcccac tctgagcaca ctgcagcaaa gacacccaca gggcccttgt 2220

cctccacctg tgacaccact gagagtccag tctgtcctgc tgctgggagt gcctcagaca 2280

gctgctagac agcctagcag acccaccggc agaaccccta ccacaagact gatggcccct 2340

gtcggcagcg tgatgagctg ttctctgctg acccagcctt ctccagccag cgcctgtaca 2400

atgcctccac tgctgcatcc tcagtgggcc ccacattctg ctggattgtc tcccggcgct 2460

tgcagacctg cctgtacctg tatcagcatg accaccatct ccagagccac ctggcaggtt 2520

tccgtgtaca gcggcaatct gctgagcttc tccagacggg gcatgaagag tagcgtgcgg 2580

ctgaaaaaca gccggcggaa cagacggtac agaaagatct acggcgagct gaagatgtcc 2640

ctgcaggccg tggctcctag atgtcctaga tctgccgtgg acatcagccc cggacctacc 2700

tctcctagcc tggcctcttg tgcctctgtg gccattggaa aactggccct gcttggcgct 2760

tctgcccctt gtggtgctgc tggcgctaga gaggctggaa gatttgccgt gctgggcacc 2820

atggtcatga agcacctgaa ccgcaagctg catctgaccc ggtcctgcct gatcgatctg 2880

accatgatga tgagccagaa gctctggaag aaccagcgga agagaatccc tctgccatct 2940

cctagacagc atctgctccc tcagtaccct ctgcaccctc ctccaccttg tctgctgctg 3000

3060

tccatcaaga tctggatcag ctttagccac gagcagagca gcccattcct ggccagatcc 3120

3180

cactgtcagt acaagctgac ctacaccaaa gtgtggaaga tgaattggaa gaaaatgatg 3240

atcggctccc agcacctcct gttcagccct ctggatagct ctccactgca actgctgctt 3300

caccctcacg gcaagagatg caacccttgc tgcagactga catggatgtc catgctgcag 3360

aaacagtgct gctacaacct cgtgatcaag cacccactga tcttcagaat caacctgcgg 3420

agacaggca agaccaagga tggcgttgtg cagagcctgg gcttcctgaa ccagcagatc 3480

atcctgcaga aagagttctt catctgcctg ctcatgttta tcaagcggtg gaagcctgcc 3540

gccaccaaga gagagtggga gaaacgcaag cagatcctcg gcggaggcgg caagtacaaa 3600

gagtacttcc tgaagcgcat cctgattcgg aaggccatga ctgtgctggc cggcgacaag 3660

aaaggcctgg gcagattcat gagatgcgtg cagagcgaga caaaggccgt tagcctgcag 3720

ctcccactcg gaagaggccc tgtgtactac gtgtgcaacc ggatgaacgt gtccagcgtg 3780

ctgaagccct tcctgttcac cgtgcctatc gaggacatga gagagtgcac actgggccgc 3840

aacagaatga atgtgtcctc tgtgctgaaa ccctgtctga tccctgtgcc aatcgacctg 3900

cctcggatgc tgccaatcag cagcccactg cacagacccc agatgccatt ctggacctgc 3960

acctttcctg ccacaacatg gggcacctgg gagacacctt ctacatgggg ctggcggacc 4020

tttcggggcc ctagaagaaa agtgctgatg gtgctgtggc tgcccgccaa ccagagtctg 4080

cagaaaagcc aagtgttcca gtgggtcctg cggaccgaga acatccggaa gctgatctgg 4140

accaagcagc ggagcttcct gtctctgttc cccaagctga gagccaccga acagatgacc 4200

aatgtgggct gcgccttcag cggccggatt ctgcatcaac ctgaaatggc cgccatgcgg 4260

ctgatgccca agttcctgaa tagcaccaac agctggtgga cctgtaccaa cctgagcgtg 4320

cccatgatgc tgaccattct gatttggaag cgggtgttca ttctcctgct gtccgacaag 4380

aagctgctga agaagtctag ccagctgagg cggtcttgga gaaacgtgtc ccggatcagc 4440

caccagatga agtgtctgac cgccgtggac tgtctgcagg ccttctggga catgaacatg 4500

gaacaccggc tgaccatgga cagcgccctg agcctgacaa agagagaggg acccgagact 4560

tgcaagaaga agctcctgct gaaactgctg acaatgagcc tgagcaacat cctgatgaag 4620

ttccggctgc ggctgagaat cccaagcctg aggaagtatc gccagcatct gaaaaagacc 4680

agcgccaagg gcgagaagct gaagcactgc tttaaggctt ggggcagagg cccacctctg 4740

cacacaagaa gccagaagaa agaccctgcc ggcaagctga aaaaactgac cacactgagc 4800

ttcgtgctga tcgaggaacg gcggaacctg cagcggaatc tgaaagtcct gtggctcaag 4860

aaaagcacca agagccggta tctgttcttc ctgaccgtgt gcggcttctt cgagaagaaa 4920

cggcctgagt ggctgtcctc cagaaagaag ggacacacaa agaatcggag gaaggccaag 4980

cggaagcccc agatcgtgca caagaagaga ggcctgttcc tcaaaaagaa atacctggtg 5040

cagggcgcca agaagatcac cttcctgtcc aacggcatgg aagcttgcgg cacacacccc 5100

acaccaagac tgagcgacag agtgttcctg gtcaagatca ccaccaccac aagcagcccc 5160

agaagaagcc cagcactgag agctagaggc ggcaccacaa ttggcgaagt gaccagcctg 5220

tgcttcttct acctgctgta cggctacccc tacgatgtcc ctgactatgc gtcctaaagg 5280

cgcgcctagc ggccgc 5296

<210> 1150

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 4A cloning cassette

<400> 1150

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgatccaga agaagaaaca gaaatccatg gccaagaagt cccagcagcc tctggtgctg 780

aagaagaacc ggaagcaaga gtggggagga caggcccaca gacagagaaa caacgccaag 840

aagatgccct tccaagagga aaagaagaac ggccggatca gcagcagcct gatcctgagc 900

ttccagagag tgaaggacag caagaaaatg acccagaaga tgtgcagctc tctggccctg 960

cctacaggcc tgacatctct gatcctgcct agcagcgatc tggccgtgct gatctctagc 1020

agcaccagcc actttctgat gcggagccct gtgctgccaa gctccagact gacatgtgcc 1080

tctccacagc tgccccggat gtggacatgg tctagctggc tgaagcaccc cagaaccgct 1140

agcatcacac tgcctaccac aagcctgaag ggcagcagaa ccagctgctg cccaagatct 1200

caccccagac tgcctaggac caagtggcct ctgcagtgtc tgctgcacag cctgggaaca 1260

acaggcacac tcgtttggga aaggggcgtg ctgcagatga gaagcctcga agcccattgt 1320

cctctgcaga ccttccatct gcaccactgc agaacccagc caagctcttg tcctcctgcc 1380

tggattctgt accaaggcca gtggagcgga gccgtcgttg gacttagacc cgtggctcac 1440

catctgccta gacctatcca gggcctgcac tgtcctcagg ctcctcatca acctgctctg 1500

agctggggaa agtggcccgt gggaatgaca ggacctgcca gatgctgtcg gtggccccgc 1560

agatctcaga agtctcagca tcactgcgcc ctgcggaaga tcgagtgggc cacaaaactg 1620

tctctcggcg gaaaagccgg accacaggct caagagaagg ccagaccttc tccttgcctg 1680

tcctggtcca gcagaattgt ggcccctgga cacgccctga gctgtcggac tcgtagaaga 1740

tggtcctcca agtgcagagg cggccctagc gccagcgata gatgttgtag ctgtagcccc 1800

agcggcttca gcgccggaag aggcagatgt cctgtgcagg gatgtctgag gccacacaga 1860

gttcagctgc tgagaagatg gggcccagga tctcctgctg gccagagact gtctaagggc 1920

ttccaactgc tgcgttggtg gggacctgga tctccagctc ctgagcctag aaagggccca 1980

tttcctccac ctgatcctcc atggcctgtg accgctgtga cagtgatggc tggctctgtg 2040

cctagcgcac agtctgtgga tgccctggaa tctcctggac ctctggctct ggaaggccct 2100

agcagcccta gaaacctgct gtggcgggaa atgagcatct ttctgcccgg catcttctgc 2160

caaaagaagg tgccatctgc cagcccctgg ggacttgttc ctccttctcc accactcgct 2220

ctgggcccta gaatgcagct gtgtacccag ctcgccagat tcttccccat cacacctcca 2280

gtgtggcaca tcctgggacc tcagagacac acacctcagg tggtcctgag actgggcaac 2340

ctgcctccat tcttcagagg cagcgaggtg ccctttccta gaccttgccg gaccaagaac 2400

gagggcaaca atcactggcc cagagtggaa atgcctaccg gctggctgct cgtgggctac 2460

2520

2580

ttctttttca aaacggtgtc cctgtgttgt cccggctggt ccaaaggaca acctccacct 2640

cctggcctga agccttctag cctggcttct caggtggcca gaaccacagg cgtgtaccag 2700

cacgtgtggc tgatctgctt cgtgttcttc gtgtgcgtgg aaaccggctt ctgtcacgtg 2760

gcacaagtgg gcctcgagag actggtttct ctgagcggca tcaccgacgt gaaccattgg 2820

gcccagccta agccatctcc tagcaaaggc accggcatga acagacccat cggcctgaga 2880

gttggcaaag gcgtgtgggc cagactgctc aagaagaagc ctagagacat ctgtcggatc 2940

tccaacagaa agacccggaa ggccgccaga gagaagcacc gcaaaacaga tagagccagc 3000

3060

ctgactctgt ggcctcctag atgcagatgc agacgcgagt acagagtgac catgaacatc 3120

ctgaacagcc tgccaagcag catggaaatc gccgtgctgc aagaaaccgc caagaaaagc 3180

cctaccagct tctgctgcaa cctggacctg cagcatcagc gcagacgcaa aaagaagaaa 3240

gacaaggtcg agatccggaa tcggagcccc aaggccaata gcacagagtg tcagggcgag 3300

cacagcagag agcagaatct gctgaatctg gtgcagctga atcctcacag ccctcaccac 3360

cggaaggacg aagaggacca ccagaagaga caccaccacc acaatcaaaa gaagatgagc 3420

gtcccactgt tcttccacgg cgacctgtgt agagagagca ccctgggcaa tttctggatg 3480

aagctgctcc agctgcctca gaggcctcca ctgcaaccag tgaccggaat ggaaatcctg 3540

ctttggattc tgctgaagat ggaaattcag atcttcaaga tttgcctggg cgagatgcag 3600

3660

aagctgtacc acagcctcgt gatgtgcctg cggctgagga ccaagctgga aaaggctctg 3720

agcgccctgt tcatctggcc tcagcactcc tacaagatca cacacccatt cgccagccag 3780

ccactgggac aagagatgcc caacaacacc cagatccggc tgctgaagcc cgacaagaag 3840

aagaaaagca ccgaaaagac caaaggccag gctttcggca gcttctccgc cgatttttca 3900

gcccctcaag gacaggcttt tggctccctg tgtcagctgg tgtccgccga ctttagcgcc 3960

ccacaagtga ccagactgcg gagcctgaac catctgctga tttggctgaa gaattggaag 4020

cggttcaagc tcaaaaagaa atatcaggcc cctctgcggc tgtggtcttg gtgcggaaca 4080

agccagacct acttcggcag catcatctgt atcaagaata tgttcctgca gcggaagggt 4140

ttcctgaacc tgagccacgt ggaaaagttc gagcttaaga agaatcttct gctccccgtc 4200

gtgatcaacc tgaagagact gcagaaattc atgctgaacc agaagtgcct ggctccattt 4260

cctgcctgca ccagcaaagc ctggacaccc agaaaactcg tgggcagagc cgtgcggaga 4320

aagggcattc tggccatgac actgaccctg aacggaatcg aggccctgac agccctgttt 4380

acctgtagaa ccgcctccat cagcgtgaac atcagcaccc tgatgagcat cctggaagcc 4440

atcatcaaga agatgaagaa acggatcatg gtgcaccggt ggatcttctc caatcagctg 4500

accgtgcaga tgctctgcct gcctctgctg acccctgaca ccagatggat ctttagtaat 4560

cagctgcaga cagtgcaaat gctgtgtctg ccacctctga agcagggcga gagaatcatt 4620

tggccactcg gcggctttca gatccccaat ttcttctggt ccctcggcat cggacacact 4680

ggcgcatggt ggcatactgg tagaaggcgg agagagtggg gcggctctac cgtgtctaga 4740

agcccactgt ggatcagctc catgatctac ctgagaggca tggccttcgc cagcgacagc 4800

tccatcagaa ccaccagagt gtcctggtgt cggagatgtg gcgccaagtc tgccgcagcc 4860

tcttcttctc cactgtggga ttgcagcacc acaaccggca cacaccacgg atgcctgtgg 4920

gagatcccta gcgttaccct gctggctaac ctgcacctgt gctactacca ctctcagagc 4980

aacagaaaca agagccgcca gatggaatcc ctgggcatga agctccaact taaaaagaaa 5040

gtgaacgcct gcagcaccta cacaccccac atctgcagac acacctgtcg cgtgaacgcc 5100

gtcgaggaac tgcactctag aaggatgtcc gtgcggatcg tgaaaaattg ggtgtcctgc 5160

tgctacgtgc ggctgtcagc tgtgggactg tctacttgga gcgctctgga caacaagatc 5220

atctgcaagg gccacagaat caccggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1151

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 1B cloning cassette

<400> 1151

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgaccgaga tccaattgac catgaacgac agcaagcaca agctggaaag ccccgctctg 780

aaacaggtgt caccagcctc tccacctact cagcagcctc agacacctca ggatagcaga 840

caggtgctgg ccatgcctag cagctggtct agctctggca gaacccagcg gaaggtggtc 900

aagaatccta gccagcctgt gcctctgaga aacgacggca tcaccaacgg caacgactcc 960

accagaagct gcagcatcag cctgaagaaa agcctggaaa agatgcccct catgatgaag 1020

aagaaggaca gcaccatctt catcgtgccc atgagcctgg cctgtagcat caccgtgtct 1080

ttcgtgggcc agatcagcac actggtgctg gatgtgtcca gcatctccca ctggaacaaa 1140

gtgcggcaga acttcaagcg gaacgccgac aagaagaacg ttcggagcgg cgagaagaac 1200

gacctgcagc tgtttgtgat gagcgacacc acctacaaga tctactggac cgtgatcctg 1260

ctgaacccct gcggcaatct gcacctgaaa accaccagcc tgggcgactg tcctagaaca 1320

atggccggac cttctgcctc tctgcaccct gcatctggca gagcctctag aaagacctgg 1380

cagagaaggc tgagagcctg tagagctggt ggacctgctg gatggggccag acaggccttc 1440

gataagaaag gctttgtgat cctgagcgtc ggagccggaa caggcggagg acctgcttgt 1500

1560 gttcctgtgg ctgaagcttc tacaggcgct tggacagctg

tgtagagctt ctagagcccc ttgcagacag ctgcctgcca gctctcctac caagtgcgag 1620

atctgcgtga agcgcagacg gcggagaaag agaagcagcc agtctagaag ccccgtgcct 1680

aagaaacgcg agagaatgat gctgcccgtg cgggccagaa cagctagatc tgcacttcct 1740

caggggagag cccctcttga gggaatgctg cttcctagag cactgcccag cctgctgtct 1800

agaatgccag ccagaagaga gagccggttt tggctggctc tgcaggcact tggactcaga 1860

cctccacatc tgcctagctg tcatcctctg cctcaactgc ttggccctcc acctcctcat 1920

caggccccta cagctttctt cctgctggac atcattctgc ggctgccctg caaagtgacc 1980

gagtctagca atatgggcgg caacagatcc atctgcctga ccaacgccaa gaaaatggaa 2040

gatatgatct gggagagcac gcccctgagc atcagcagac ctacatctag agccaggcag 2100

agctgttgtc tgcccggact gacacatcct gctcaccagc cactgggatc tatgaccctg 2160

gccatcagat tcatcagcgc tcctcagcct agctccagca cagtgacagc tgctcctagc 2220

ggccagtact tcaagggcaa gaagcggcgg aaagagatct tcaagaagaa gaaacccctg 2280

acattcagcc ccagcctggc caaaaagacc ctgaccaata gcagcttctt ctacaccagc 2340

gaccgcgagg ccaagaaatc tatgaagcgg agcatgaccc tgaaggccct ggtcaagacc 2400

tggacacagt tcagaaacta ccccagagcc tcctctcctg gacaccctct gggacctaat 2460

ggcatctggg aagtgatgcc cagaacactg gaaggctgca tctccgtgaa ggaccacaga 2520

acaagccaga accacagcaa gtgcatcacc gagtgtgtgc ggacagccct gaacagaaga 2580

agcaagatcg aggcctctcc tatcggctct atcgccctga gatccatgcc taagagcaga 2640

aaccaggtgc tgttcagacg gaatctgacc ccaagccagc tgagaactct ggcccctcct 2700

tatatgctgc tgccccagtc tctgagccag agcctgagca gaaagcagat ccctagccag 2760

2820

atgagcctgc agtggaagtc cggcaatgcc tgcacacctc tgagatgccc tagcagaagc 2880

cctgccacat tcctgacagc tctgaacggc tggcacagat ctggactgag agcccagaga 2940

aagcgcaagc gcgagaagag aagccagaag tccaggcaga accatctgca gctgaagtcc 3000

tgccggcgca agatctccaa ctggtccaag ctgagaagcc cctggatctt cgtgtccaag 3060

aagagactgc ccaagctgac caagtgtgaa atctgtgtca agaacaccgc catcctggct 3120

atcaccctga tcacccctca ggaccctcat ctggaccctg tgtgtcaccc tcatagaagg 3180

cctggatgga agggccacca cagagtggtg ttttctcatc accctagcgg cagcaccaag 3240

agccactggc agcaaagaga acgcggaggc ggcagacggg aaaagaaggg ctgtagagag 3300

agatccctgc tgtactccag aatgtgcctg gtgcagatcc ccacacacac ctgggagccc 3360

gattacttcg tgtctctgcc catgatgttc agctgggcca cacagtcatt ccccgtggga 3420

gccgtgtttc tgaccagacc tgccattcct tgctgtagct gcacaagcca cagcaaccct 3480

tggagtcaga gcagacaccc acacagaagc ccacctacct cttgtcctcc gctgctggaa 3540

gcctttccac tgcctagagc atccctgaga gccaccgtgc caagacatag ctctgtgctg 3600

gcaaccggac tgcatctgca cagacagatt tctctgcccc tgagaggatt cctgctgcat 3660

caaccactgc caggccagca tctgtggctg tctcatagac tgcggctgag actgccaaga 3720

actcagcggc ctcctagatc catcctgagc cttagactgc cactgaggcc acagaggagga 3780

cccagacctc tgcatagatg gctgcagcag accacaggct gtagccagag aagatacccc 3840

agactgaaaa ccttcggcgt gccactgggc tccattctgt gtctggctgg atctctgagc 3900

accatgatga tcattagcac caccagacaa gagtgtggca ggcgggcatc tgtgccctgt 3960

agacctatga tcggaagcgc cagaccagga ccttggagaa catctgctat gcctagcgcc 4020

atgggcgtcg cactgcctac aagctgtgaa tccggaagat ctcctcctgc caccggcgga 4080

agaatgcctg gaagccagag cattatgatg agctggatgc ctcctctggc tccttgtgcc 4140

gcttgtcctt gttctcccgc tcctacactg tgtcccgctc atcctgctag agcacctaca 4200

gccgtgcctg cctttacacc tctgtctgca catcctgtgc ctgtgctgtc tggatgtcac 4260

ctggccgtca gaacctccat gctgacactg ctgcctatgc tgaagctggc cccactgcaa 4320

gtgaccacca cactgaagct gatcctggtc atgctggaaa tcgtgaccga gagcactcgg 4380

agagagatgc cttctcaggc cacaccttct ccacctggac cttggcgagc cgctctggggc 4440

aaagaagtga agagatccca gagcaagctg cgcctgagaa acacaagccc catcgtgttc 4500

tttctggccc catacttccc cagacggatg accagacgga ccatgacaac caccaaaatg 4560

atgatgatgc ggcggaccaa gcggagaagg cgcaaaaggc ggagaatgac aatgatgaca 4620

aggcggaccc tgctgacaaa gaaaacctgt ctgaccccta gaaccagcgc cgctaccaaa 4680

gctctgccaa ggcagacaat ctcccagcgg aggcctattc agacccctcc tggaacatct 4740

ctggccatct ggggcaacca gcagctgaaa tggacccctc acatcctgaa gtccgccagc 4800

agaagggcca gacctaccag ctgtagcacc tgtagaatgt ggtggatcgc caaaaagcgg 4860

atcaagctgc ggcggaacag aaccagactg acactgacag gcacaagaag cagcggcacc 4920

agagcttctc catccgccgt ttgcctgtgt tgctggacct gtccttccag aagcttccca 4980

gccgcttctc caagcggatt gagagcacct gttggagcac aggcccctcc agaagttaca 5040

ctgagagcta gctgtgcctc cagcgcctct aaggcttgtg ctggactttg gccttgggga 5100

gcacaagttc ggcaaccatc tttgcctgga ccatgcgctt ctctgctgag cacactgtct 5160

cagccacctc cacaggctcc atctcaagtg tggacagccg caacactgag atgtcctgct 5220

gttccagctg ctgcctgtcc tcctggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1152

<211> 5281

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 2B cloning cassette

<400> 1152

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgagaaaga tgcagagcct gaatcacccc agcagactga ccaacatgag cagaacacct 780

ccaagacgcg tgtgcaaccc tcggagaaag acctggaaga tgcggaagaa gtacgcccag 840

aagcctagcc agaagaacca cctgtacaac agccggaaga aactgctcga ggacatgaag 900

cagaatggct tcacaggcca cagaggggc agcgagtctg ataatctgggg cctgctgtac 960

tcctttcctc ctatccggat gtgcgccctg tctcacccta gcttctacat cagcagcacc 1020

gtgtgctttc tgagcaagca ctgcatgacc gcctgcacac aaaagaaagg ccacatgatc 1080

ttcagccgcg agaagatcat caaccccacc atcagctgcc cctttcagag ccccacaaag 1140

cggctggaac tgagcctgga accttggatt ccctacctgc accagcagaa aacccaaggc 1200

ggcaagaaga gaagcaagtt ccctggcacc agcctgcagt gctacctgaa cagatgcagc 1260

ctgatggaac agatccctca cctgaagtcc gcctctccac agatcatgca gagaaccagc 1320

gccgaccggt tccggatgaa gcggagaatg cagaacctgg acaatctggg cgttccatgg 1380

ccactgctgc ctggacctct ggaaaacaca cagatcccca gaacaagccc tctgtggaag 1440

atcacaatgg tgctgacata ccccaactgc gtggtgcacg tgctgatgtt ctggctgcct 1500

atgagatctc tgggcgagcc ttggagagct ggctttcagt ggggagagtc cccttggcaa 1560

gtgatggcta gcctgacagg cccctatcct atcagcgagg gcctgaccat gaccaccggc 1620

agaaacatca acagcaactc cctgatgacc aactgcctgt tcagccccat cttcctgtgc 1680

caccacgcca tcatgcaccg gaagatgaca agcccacact tcagactgtt cagcagcaag 1740

atcccacatc ctcaggtgcc atctgtggtg gccctgtgca agttcagagc cacatttctg 1800

ctcgccctgt gggagtgtag tctgcctcaa gccagactgt gcctgatcgt gtctagaacc 1860

ctgctgctgg ttcagtctgc cgctcctcca catagactgc tgggcccttc tagatggtcc 1920

agaagccacc tggacttcag cccctactgg atgaagaaag tgaagctggc caacggcctg 1980

tggttcaacc tgatcttcgt gctgttcaag gccgaccaga gcgagtctag cctgtctagc 2040

ggccggaaca agaaaacaaa gctgtggttc tccctcatca acatccacca ccgcaagaat 2100

cctctgctgc ccatgagaat ccccagcctg aatgcccagt ggatgttcct gagaatcctg 2160

cttctggccc atatgtacgg cttcagcacc tacggcaccc agaaaggcga gtgcgtttgt 2220

ctgcagtgtc tgagcggcag cagcctgttc attctgaagc agcccctgga cctgaagatc 2280

tccaagacct tcatgctgca agctcaccca caggtgccag ctcttgctcc tcaagcttgg 2340

tggcctgcta gaagaggccc tctgacatct gctccatccg ctcagcagtc tctgacagct 2400

agccctcaga ttatgcagcg gatgagcgcc gtgaacttcg agacaatcat ctaccctcca 2460

agcacctgta gcctgccttg tagaggcaga acactggaaa gcgccggact gtttccttgg 2520

ctgcacaccc agtgtacaca cccctgtacc atgctgctgc cacaaggctg gtggtgtaca 2580

agaggcggcg gaaagcggaa aaagaaaatg tgcaaggtgt ccggcggctg caccgcctgt 2640

gttgagagat gtccttggaa gggcatctgc tggaagtggc cagtgacagt ggctgctcag 2700

tggtctagcg tgacctctct ggaatggaca gcccaagaga gaggctgcag cagctggctg 2760

atgaagcaaa catggatgaa gtcttggtcc ctgcgggacc ccagctacag atccatcctg 2820

gaatacgtgt ccaccagagt gctgtggatg cccacaagca ccgtgcggag aagaaacagc 2880

agaaagcagt ctcaggtgca gctggccatt caggtgctgc tcgtggacaa gcacaagtac 2940

tttctgttcg gcagagcccg caaccggaac atgaagaaaa ccatgagcca cctgatcgcc 3000

catcaggccc tgaagatgag cggcctgagg ctgtttcatc ctcggctgct gacaagaaag 3060

aagcccggca agttcctgcc tagcagattt ccttggggcc ctcctcctct gtgtcatcct 3120

gcttggctga gccctatctc cgcctgtcca agactgctgc catatccttt tcacgtgctg 3180

gccctggaag tgacctttcc acctggaaca gccatgcctc agatgcaact gcaggccctg 3240

gccatgtggg agaaaggctg ggataagcgg aagagaatga agaccggcgc catccaagag 3300

3360

gcctgcagag agatggaaag cctgagcgcc

atggcccggt gcacctccat caatgtgctg tgtgtcagag cctctctgat cgagaagctg 3480

3540

ctgttcatta agggctacca gaacaaccac ttcaccttcc atctgcaaaa cttcgccttc 3600

ttcagcagct tcggcaacaa caagtacatc aatatcctga cgcggcgcaa gaaaagagag 3660

gaccactgcg gactgctgat cctgatccag cacggcggca tcttcacctt tcggttctgg 3720

gtcgaaaagc tgagctggaa catctgcaag aacctgagcc tgtacctgga taagttcgtg 3780

cagcggctgc tgtacgtgta catcatcgag acaaacgtgt tcgtgctgaa cctgttccac 3840

gtgccagtga accagatctt catgatggcc ttctacctga agtacaccga gaaggcctgg 3900

gtcatggaac tggaatggct gatccaacag cagtgttacc agcaagagaa gaaaagccgg 3960

aaactgatga gcagctgccg ggccttcatc cacacaccta gaagccctgg ctgcagcgtg 4020

tacgccaata tgtgctgggc cttttggaag cctcctagcc agggcatcag ctcttgtctg 4080

atgacaggct gttggggcag ccctcctctg cacctgtgtc agcctctgag gcggtataag 4140

aaaaggggcg tgatcgcctg gcggcggaga aagtggcaga tccggcatgt gctgaaaaac 4200

ctgatcctgt ttcatgtgcg gaacatcgtg tacggcatcc aagtgatgct gtgcggcaag 4260

ggactgacac accctgtgcc tctgaaacgg accctgcaga acaagaggaa caagaacatc 4320

aagcgggtca accagaagaa gggcaacatc aagggaagt ccatcctgct gcacctcatt 4380

gtgctgaata gccccgagag caaccccaag ttcttcctga cctttagcca gctcgagaag 4440

aaagctacct ggatgagaag aacaggcgcc ctgagagcca cagctcctgc tacagcagct 4500

cctcaggctc agactcctca ttggagagcc gccacaacaa gcagcatgag cagcagccct 4560

agagccgagc ctacatcttc tagctcttgg gccacctgtt ggcccagatg ctgtagctcc 4620

agctgttact ggtccctgag ttcttggcct cctgctggcg ccgctgaggc tacaaacacc 4680

agaactagaa gcagagagtc ccagcggggc agaatgctga cttggagaag tagtctgtgg 4740

4800

tgtagctgcg gaagaggctg tggaacccct agaggcgatt ggaagagaca ccctcctcgg 4860

aggaatagcg tgatcgtgca caagatcgtg ctggtgtttc tgaagtccct gtccagctac 4920

aactgcgaga gaaggatcag cggcatccag aagagagcca tcgagtttgc cgtgctgaaa 4980

ctgctgaata tggacctgca ctggaactcc gcctacctgg gcagaactct gctggtcacc 5040

tgtatcctgt atcaccggtg gatcgtgacc tccatgtgcc agtctcagag atggctgacc 5100

agcaccacaa gcagatccag cgctctgatg tggacccggc agatcaatcg gaggaagctg 5160

cagagaaaga agaggcaccc aagctggcct tggaccagat gccttagaat gaggcctcct 5220

agaagcggct acccctacga tgtccctgac tatgcgtcct aaaggcgcgc ctagcggccg 5280

c 5281

<210> 1153

<211> 5296

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 3B cloning cassette

<400> 1153

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgggagctg ctgctgcctc ttgtcagtct cctccttctc ctcgggaaac cacacctagc 780

tccgacgcca ccagacggac aagaccaatt gccgctcaga gcaagaaaac catgacagcc 840

gccggactga cccctaagaa caagaagcgg agaaagctgc ggcccaagat gatccggaag 900

atccagaaga gaaggcggac cgccaaccgg cggaatagca gaagggctac ccctacaagc 960

aagggcagcc tgagaagaaa gtacctgaga gtgcggggac ctcctctgca cacccggaac 1020

atcagaaagc tgatctggac caagcagcgg agcttcctgt ctctgttccc caagctgaga 1080

gccaccgagc agatgaccaa tgtgggctgc tctatccctc acgtgcccaa agccacagcc 1140

gctcactctg tgacagtgaa actgcagctg cccctgacct tcagcaccct gctgtgggat 1200

agcagacaca ccatgagcct gagcaacatc ctgatgaagt tccggctgca gtctagccca 1260

ttcctggcca gatcctggaa caccatgcag tggaacaaca agaaaatggc catgaccgtg 1320

atcgctggcg cccaccactg tcagtacaag ctgacctaca ccaaagtgtg gaagatgaac 1380

tggaagaaaa tgatgatcgg cagccagcat ctgctgttca gccctctgga tagcagtccc 1440

ctgcagctcc tgctgcaccc tcacggcaag agatgcaacc cttgctgcag actgacctgg 1500

atgagccaga acaccagaag caccaacctg gaaaagaacc ccagcctgaa gaagcagagc 1560

cagcggagag gaaagctgag cgcctttagc ggcagaatcc tgcaccagcc agaaatggcc 1620

gccatgagac tgatgcccaa gttcctgaac tccaccaaca gctggtggac cctgaccaca 1680

ctgagcttcg tgctgatcga ggaagccaga cagcccagca gacctaccgg cagaacacct 1740

accccagac tgatggcccc tgtgggcagc gtgatgagct gttctctgct gacccagcct 1800

tctccagcca gcgcctgtac aatgcctcca ctgctgcatc cacagtgggc ccctcatagt 1860

gctggacttt ctccaggcgc ttgcagacct gcctgcacct gtatcagcat gaccaccatc 1920

agcagggcca catggggccc agtgtactac gtgtgcaacc ggatgaacgt gtccagcgtg 1980

ctgaagccct tcctgttcac cgtgcctatc gaggacatga gagagtgcac cctgggcaga 2040

aacagaatga atgtgtcctc tgtgctgaaa ccctgtctga tccctgtgcc aatcagcgag 2100

aagaagagga agctgcccaa catcgagatc acccctcagc ggtctaaggc catgctgcag 2160

aagcagtgct gctacaacct cgtgatcaag caccctctga tcttcagaat caacctgcgg 2220

2280 cggcagggca agaccaaaga tggcgttgtg cagagcctgg

atcctgcaga aagagttctt catctgcctg ctcatgttca tcaagcggtg gaagcctgcc 2340

gccaccaaga gcagaaagaa gggccacacc aagaacagac ggaaggccaa gagaaagccc 2400

cagatctacc tgttcttcct gaccgtgtgc ggctttcacc tgaaaaagac cagcgccaag 2460

tggctgaaga agtccaccaa gtccagatcc aacggcatgg aagcttgcgg cacacacccc 2520

actcctagac tgagctgcct gagagttaga tgcggcggct ggctgtgtgg ctggacacct 2580

agaggtagaa agtccggcgt gatgaagtgc tgggatctgt gccccgtgca caagaagaga 2640

ggcctgttca gactggattt tcaccccaga agcagctccg gcaagggaaa gaacagcccc 2700

tgctgccaaa agaaactgcg ggtgcagaac cagggccatc tgctgatgat tctgctgcac 2760

aatgaccggg tgttcctcgt gaagatcggc gagaagctga agcactgttt caaggcctgg 2820

ggctccagac ggaaccggcg gtacagaaag atctacggcg agctgaagat gtccctgcag 2880

gccttcctga tccacctgag aaaggtggac acccaccacc tccaggtggt caccaaaaca 2940

gtgaccgcca caacagccga cggccagaga atgcggagac tgaagtgcaa ggcctgccgg 3000

ctgagatggg ccagacctga acctgctgct caggcccctc ctaagagcca ctttccacct 3060

catcctagcg gccccagcag aacaagtagc ctgcagctga gaagcctgtt ctttagccac 3120

tgcctgtgca gccagaagaa ggaccctgcc ggcaagctga aaaagctgct gaagaaaagc 3180

agccagctgc ggagaagctg gcgcaatgtg tccagaatca gcacccctcc tccactgatc 3240

ctgtggcctc aagctgctcc tgtgtccgtg atcaccatga agctgtggga gaagcacttc 3300

aagggcctga attggaacct ggtcgtgtgg attctgaacc tgaagatgat gacctacatg 3360

gaaatgcact acggccatca ggccccacag gtggcaatgc tgcctaagct gctgtgcaga 3420

cagatgctgc ccactctgag cacactgcag cagagacatc ctcagggccc ttgtcctcct 3480

cctgtgacac ctctgagagt ccagtctgtc ctgctgctgg gagtgcctca gacagccaga 3540

ggacctagaa gaaaggtcct gatggtgctg tggctgcccg ccaatcagtc tctgcagaaa 3600

agccaggttt tccagtgggt gctgcggaca gaggtggccc ctagatgtcc tagatctgcc 3660

gtggacatca gccccggacc tacatctcct agcctggcct cttgtgcctc tgtggccatt 3720

ggaaagctgg cactgcttgg cgcctctgct ccttgtggtg ctgctggcgc tagagaggcc 3780

ggaagatttg ctgtgctggg caccatggtc atgaagcttt gtttcttcta cctgctgtac 3840

cggctgcgca tcccaagcct gaggaagtac agacagcacc tgaaccggaa gctgcacctg 3900

acaagatcct gcctgatcga tctgaccatg atgatgtccc agaagctctg gaagaaccag 3960

cggaagagaa tccctctgcc atctccgg caacatctgc tgccacagta ccctctgcac 4020

ccacctccac cttgtctgct gttgctgcac catagacacc acctggcctt tctcgagatg 4080

gcctgtagct ctcagcacac ccacagcatc aagatctgga tcagcttcag ccatgaagtg 4140

gaccctccag gcagcgtgtg gatactgcct tgcgatctgc ctcggatgct gccaatctct 4200

tcacccctgc acagacccca gatgcctttc tggacctgca cctttcctgc cacaacctgg 4260

ggcacatggg agacaccttc tacatggggc tggcggactt tcagaggcgc tagcagcagc 4320

acatcccggg acagcagatc cttcttcacc tctgctcacc ctagcaggcc ctgcagaatg 4380

agactgtacc agtgcaagaa gaagctcctc ctgaaactgc tgaccaccac cacaagcagc 4440

cccagaagat cccctgctct gagagcaaga ggcggcacca caatcggcga agtgaccagc 4500

tgtaccaacc tgagcgtgcc catgatgctg accatcctga tttggaagcg ggtgttcatc 4560

ctcctgctga gcgataagaa gggcgccaag aagatcacct tcctgcctat tcctccacac 4620

ctgagacacc ctctgctgct gttcgagaag aaacggcctg agtggctgag catggacctg 4680

gacggaatgc tgacagtgat cggccaggga ctcgtgggca tgacccctag agatctggga 4740

acagtggcag gcgccaccgc tcagattcag cgacctatcc aggtgcagac tggcggcaga 4800

ctcagaagaa acctgcagag aaacctgaag gtgctccagg tgtccgtgta cagcggcaac 4860

ctgctgtcct ttagcagacg gggcatgaag tctagcgtgc ggctgaaaaa ccgcgagtgg 4920

gagaaacgga agcagattct cggaggcggc ggaaagtaca aagagtactt cctgaagcgc 4980

atcctcatcc ggaaggctat gacagtgctg gccggcgaca agaaaggact gggcagattc 5040

atgagatgcg tgcagagcga gacaaaggcc gtgtctctgc aactgcctct gggaagaaac 5100

atggaacacc ggttgaccat ggacagcgcc ctgagcctga caaagagaga gggacccgag 5160

acactcaaaa agaaatacct ggtccagcac cagatgaagt gtctgaccgc cgtggattgt 5220

ctgcaggctt tttgggacat gggctacccc tacgatgtcc ctgactatgc gtcctaaagg 5280

cgcgcctagc ggccgc 5296

<210> 1154

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 4B cloning cassette

<400> 1154

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgatccaga agaagaagca gaagtccaac ctggacctgc agcaccagcg cagacgcaaa 780

aagaagaagg acaaggtcga gatccggaat cggagcccca aggccaatag cacagagtgt 840

cagggcgagc acagcagaga gcagaacctg ctgaatctgg tgcagctgaa ccctcacagc 900

cctcaccaca gaaaggacga agaggaccac cagaagcggc accaccacca caatcaaaag 960

aaaatgagcg tgatggccaa gaagtcccag cagcctctcg gacaggcctt tggctctctg 1020

1080

catctgctga tcaacgaggg caacaaccac tggcctagag tggaaatgcc taccggttgg 1140

ctgctcgtgg gctacaacac cagaaacaca gcccagaatc ctcggctgca gcagcacctg 1200

aaacactggc agcagtgcac cagagccaga tggtgccaag agccttgtca gaccctgacc 1260

ttcttccatt tcttcttctt tttcaaaacg gtgtccctgt gctgccccgg ctggtctaaa 1320

ggacaacctc ctccacctgg cctgaagcct tctagcctgg cttctcaggt ggccagaacc 1380

acaggcgtgt accagcacgt gtggctgatc tgcttcgtgt tcttcgtgtg cgtggaaacc 1440

ggcttctgtc acgtggccca agtgggactc gagagactgg tgtctctgag cggcatcacc 1500

gacgtgaacc attgggccca gcctaagcca tctcctagca aaggcaccgg catgaacaga 1560

cccatcggcc tgagagttgg caaaggcgtg tgggccagac tgagagcctc cgtgtctttc 1620

tggaccctgc tgcctacaac aggcgtgcgg acaagaggaa gccctctgag actgacactg 1680

tggcctccac ggaacatcct gaacagcctg cctagcagca tggaaatcgc cgtgctgcaa 1740

gagaaccgca agaccagaaa ggccgccaga gagaagcaca gaaagaccga caacctgcac 1800

ctgtgctact accacagcca gagcaaccgg aacaagagcc ggcagatgga aagcctgggc 1860

atgaagctgc agagccctct gtgggattgc tccaccacaa caggcacaca ccacggctgt 1920

ctgtgggaga tccctagcgt tacactgctg gcctgcagag tgaacgccgt ggaagaactg 1980

cacagcaggc ggatgtctgt gcggatcgtg aagaactggg tgtcctgctg ttacgtgcgc 2040

ctgtctgctg tgggcctgtc tacttggagc gccctggacg tggtcatcaa cctgaagcgg 2100

ctgcagaagt tcagccagaa gtctcagcac cactgcgccc tgagaaagat cgagtgggcc 2160

acaaagctga gcctcggcgg aaaagctgga ccccaggctc aagagaaggc caacgccaag 2220

aagatgccct tccagcggtg gatcttcagc aatcagctga ccgtgcagat gctgtgcctg 2280

cctattgaag ccctgactgc cctgttcacc tgtcggacag ccagcatcag cgtgaacatc 2340

agcaccctga tgagcatcct ggaagcccct ctgttttttc acggcgacct gtgcgaggaa 2400

aagaagaacg gccggatcag cagccaggct cctctcagac tttggtcttg gtgcggcaca 2460

agccagacct actttggcag caccgccaag aaaagcccca ccagcttttg ctgcgagtgg 2520

ggaggacagg ctcaccggca gagaaatacc ggaatggaaa tcctgctgtg gattctgctc 2580

2640

ccccacatct gcagacacac ctgtagaggc ggacctagcg ccagcgatag atgctgtagc 2700

tgtagcccta gcggcttcag cgccggaaga ggcagatgtc cagttcaggg atgtctgcgg 2760

ccctcacagag tgcagctgct gagaagatgg ggccctggat ctcctgctgg ccagagactg 2820

tctaagggct ttcagctgct ccgttggtgg ggaccaggat ctccagctcc tgagcctaga 2880

aagggcccat ttccaccacc tgatcctcca tggcctgtga ccgctgtgac agtgatggct 2940

ggctctgtgc ctagcgctca gtctgtggat gctctggaaa gccctggacc tctggcactg 3000

gaaggaccta gcagccccag aaacctgctc tggcgggaaa tgagcatctt cctgcctggc 3060

atcttcacct ctcggattct gaacctgcaa gtgctgaaga agatcctgcg gagctttatg 3120

aagctgtacc actctctggt catgtgcctg cggctgcgga ccaagctgga aaaagctctg 3180

agcgctctgt tcatctggcc ccagcacagc tacaagcaga ggcctcctct gcagcctgtg 3240

caggtcgtgc tcagactggg aaacctgcct ccattcttca gaggcagcga ggtgcccttt 3300

catcacacac 3360

ccattcgcct ctcagcccct gggacaagag atgcccaaca acacccagat cagactgctg 3420

aagcccctgc tgacccctga caccagatgg atcttctcca accagctgca gacagtccag 3480

atgctctgtc tgccacctct gaagcagggc gagagaatca tttggcccct cggcggcttt 3540

cagatcccca acttcttttg gagcctcgga atcggccaca ctggcgcctg gtggcatact 3600

ggaaggcgga gaagagaatg gggcggctcc accgtgtcta gaagcccact gtggatcagc 3660

tccatgatct acctgagagg catggccttc gccagcgaca gctccatcag aaccaccaga 3720

gtgtcctggt gcagaagatg cggcgccaaa tctgccgctg cctccagcct caagaagaaa 3780

ccccgggaca tctgtcggat cagcgactct aagagatga cccagaagcg cgagagcacc 3840

ctgggcaatt tctggatgaa gctcctccag ctgccagaca agaagaagaa gtctaccgaa 3900

aagaccaaga tcatcaagaa gatgaagaaa cggatcatgg tccacatgtg cagctccctg 3960

gctctgccaa caggcctgac atctctgatc ctgccaagca gcgatctggc cgtgctgatc 4020

tctagctcca ccagccactt tctgatgcgg agcccagtgc tgcctagctc cagactgact 4080

tgtgcctctc cacagctgcc cagaatgtgg acctggtcca gctggctgaa gtggctgaaa 4140

aactggaagc ggttcaagct taaaaagaag tacatcctgg ccatgacgct gaccctgaac 4200

gggcatccaa gaaccgcctc tatcaccctg cctaccacca gcctgaaggg ctctagaacc 4260

agctgttgcc ccagatctca ccccagactg cctaggacaa agtggcctct gcagtgcctg 4320

ctgcacagcc tgggaacaac tggaacactc gtgtgggaaa ggggcgtgct gcagatgaga 4380

agtctggaag ctcactgccc tctgcagacc ttccacctcc accactgtag aactcagccc 4440

agctcttgcc ctcctgcatg gattctgtac caaggacagt ggagcggagc cgtcgtggga 4500

cttagacctg tggctcacca tctgccaaga cctattcagg gcctgcactg cccacaggct 4560

ccacatcaac cagctctgtc ttggggcaag tggcccgtgg gaatgacagg acctgccaga 4620

tgttgccggt ggcctagacg ggaactcaaa aagaatctgc tgctcccctg ccggtgcaga 4680

cgcgagtata gagtgacaat gggccaagcc ttcggcagct ttagcgccga ctttagcgct 4740

ccccaggtgt tgaagaagaa tcggaagcag aacaagatta tctgcaaggg ccaccgcatc 4800

acaagcctga tcctgagctt tcagcgcgtg aaacttaaga agaaagtcaa cgcctgctct 4860

cccgcctgca cctctaaagc ctggactccc agaaagctcg tggggagagc cgtcagaaga 4920

aagggcatca tctgtatcaa gaatatgttc ctgcagcgga agggtttcct gaacctgagc 4980

cacgtggaaa agttcgtgcc tccttctcct cctctggctc tcggccctag aatgcagctc 5040

tgtacccagc tggcccggtt cttccctatc acacctcctg tgtggcacat cctgggacct 5100

cagaggcaca ccccttgtca aaagaaggtg ccctccgcta gcccatgggg ccttagacca 5160

tctccttgcc tgagctggtc ctccagaatt gtggcccctg gacacgccct gagctgtcgg 5220

actagaagaa gatggtccag caagggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1155

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 1C polypeptide

507

<400> 1155

Ser Thr Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly

1 5 10 15

Pro Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys

20 25 30

Leu Arg Leu Arg Asn Thr Ser Pro Thr Glu Ile Gln Leu Thr Met Asn

35 40 45

Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val Ser Pro

50 55 60

Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp Ser Arg Gln

65 70 75 80

Val Leu Ala Ala Ser Pro Gly His Pro Leu Gly Pro Gly Asp Cys

85 90 95

Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly

100 105 110

Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala

115 120 125

Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Met Thr Leu Lys Ala Leu

130 135 140

Val Lys Thr Trp Thr Gln Phe Arg Asn Tyr Pro Arg Val Gly Ala Gly

145 150 155 160

Thr Gly Gly Gly Pro Ala Cys Val Pro Val Ala Glu Ala Ser Thr Gly

165 170 175

Ala Trp Thr Ala Glu Gly Ala Ala Gly Thr Arg Cys Arg Ala Ser Arg

180 185 190

Ala Pro Cys Arg Gln Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu Ile

195 200 205

Cys Val Lys Arg Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg Ser

210 215 220

Pro Val Pro Lys Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala Arg

225 230 235 240

Thr Ala Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly Met

245 250 255

Leu Leu Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala Arg

260 265 270

Arg Glu Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg Pro

275 280 285

508

Pro His Leu Pro Ser Cys His Pro Leu Pro Gln Leu Leu Gly Pro Pro

290 295 300

Pro Pro His Gln Ala Pro Phe Asp Lys Lys Gly Phe Val Ile Leu Ser

305 310 315 320

Lys Gly Lys Lys Arg Arg Lys Glu Ile Arg Arg Met Thr Arg Arg Thr

325 330 335

Met Thr Thr Thr Lys Met Met Met Met Arg Arg Thr Lys Arg Arg Arg

340 345 350

Arg Lys Arg Arg Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr

355 360 365

Lys Lys Thr Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu

370 375 380

Pro Arg Gln Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly

385 390 395 400

Thr Ser Leu Ala Ile Trp Gly Arg Leu Pro Leu Arg Pro Gln Arg Gly

405 410 415

Pro Arg Pro Leu His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln

420 425 430

Arg Arg Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn Thr Leu Ala

435 440 445

Ile Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala

450 455 460

Ala Pro Ser Gly Gln Tyr Phe Ser Ser Phe Phe Tyr Thr Ser Asp Arg

465 470 475 480

Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr

485 490 495

Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro His

500 505 510

Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser His His

515 520 525

Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly

530 535 540

Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Met Pro Ser Ser Trp Ser

545 550 555 560

Ser Ser Gly Arg Thr Ala Asp Lys Lys Asn Val Arg Ser Arg Thr Arg

565 570 575

509

Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser

580 585 590

Ala Val Cys Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala

595 600 605

Ala Ser Pro Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro Pro

610 615 620

Glu Val Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala Cys

625 630 635 640

Ala Gly Leu Trp Pro Trp Gly Ala Gln Val Arg Asn Lys Val Arg Gln

645 650 655

Asn Phe Lys Arg Asn Leu Ala Lys Lys Thr Leu Thr Asn Ala Leu Arg

660 665 670

Ser Met Pro Lys Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu Thr

675 680 685

Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro Gln

690 695 700

Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln Ser Met

705 710 715 720

Leu Val Thr Ala Phe Phe Leu Leu Asp Ile Ile Leu Arg Ser Leu Leu

725 730 735

Tyr Ser Arg Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro

740 745 750

Asp Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser

755 760 765

Phe Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys

770 775 780

Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Phe Lys Lys Lys

785 790 795 800

Lys Pro Leu Thr Phe Ser Pro Ser Val Pro Met Ser Leu Ala Cys Ser

805 810 815

Ile Thr Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val

820 825 830

Ser Ser Ile Ser His Trp Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln

835 840 845

Lys Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile

850 855 860

Ser Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe Tyr Pro Arg Leu

510

865 870 875 880

Lys Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser

885 890 895

Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg

900 905 910

Arg Ala Ser Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly

915 920 925

Pro Trp Arg Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro

930 935 940

Thr Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg Met

945 950 955 960

Pro Gly Ser Gln Ser Ile Met Ser Trp Met Pro Pro Leu Ala Pro

965 970 975

Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys Pro Ala His

980 985 990

Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr Pro Leu Ser Ala

995 1000 1005

His Pro Val Pro Val Leu Ser Gly Cys His Leu Ala Val Arg Thr

1010 1015 1020

Ser Met Leu Thr Leu Leu Pro Met Arg His Pro His Arg Ser Pro

1025 1030 1035

Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro Arg

1040 1045 1050

Ala Ser Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala

1055 1060 1065

Thr Gly Leu His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly

1070 1075 1080

Phe Leu Leu His Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser

1085 1090 1095

His Arg Leu Arg Leu Arg Leu Pro Arg Thr Gly Glu Lys Asn Asp

1100 1105 1110

Leu Gln Leu Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp

1115 1120 1125

Thr Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr

1130 1135 1140

Thr Ser Leu Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg

1145 1150 1155

511

Met Trp Trp Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr Leu

1160 1165 1170

Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Glu Glu Ala Lys

1175 1180 1185

Lys Ser Met Lys Arg Ser Arg Arg Ser Lys Ile Glu Ala Ser Pro

1190 1195 1200

Ile Gly Ser Ile Met Lys Lys Lys Asp Ser Thr Ile Phe Ile Asn

1205 1210 1215

Gln Gln Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser Val

1220 1225 1230

Ser Lys Lys Arg Leu Pro Lys Leu Asn Gly Ile Trp Glu Val Met

1235 1240 1245

Pro Arg Thr Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr

1250 1255 1260

Ser Gln Asn His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala

1265 1270 1275

Leu Asn Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro

1280 1285 1290

Leu Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser

1295 1300 1305

Cys Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met

1310 1315 1320

Asn Ala Lys Lys Met Glu Asp Met Ile Trp Glu Ser Asn Met Gly

1325 1330 1335

Gly Asn Arg Ser Ile Cys Leu Thr Arg Leu Pro Cys Lys Val Thr

1340 1345 1350

Glu Ser Ser Gln Arg Pro Pro Arg Ser Ile Leu Ser Leu Ala Ser

1355 1360 1365

Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly Met

1370 1375 1380

Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys

1385 1390 1395

Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp

1400 1405 1410

His Arg Ser Gly Leu Arg Ala Gln Gln Pro Ser Leu Pro Gly Pro

1415 1420 1425

512

Cys Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala

1430 1435 1440

Pro Ser Gln Val Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val

1445 1450 1455

Pro Ala Ala Ala Cys Pro Pro Ile Val Phe Phe Leu Ala Pro Tyr

1460 1465 1470

Phe Pro Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg

1475 1480 1485

Gln Ser Cys Cys Leu Pro Gly Leu Thr His Pro Ala His Gln Pro

1490 1495 1500

Leu Gly Ser Met

1505

<210> 1156

<211> 1501

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 2C polypeptide

<400> 1156

Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg Lys Lys Arg Arg Lys Lys

1 5 10 15

Arg Glu Asp His Cys Gly Leu Arg Ala Arg Asn Arg Asn Met Lys Lys

20 25 30

Thr Met Ser His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu

35 40 45

Arg Leu Phe His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Asn

50 55 60

Pro Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro

65 70 75 80

Ser Gln Lys Asn His Leu Tyr Gly Lys Gly Leu Thr His Pro Val Pro

85 90 95

Leu Lys Arg Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val

100 105 110

Asn Gln Lys Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu

115 120 125

Ile Val Leu Asn Ser Pro Glu Ser Asn Leu Gln Ala His Pro Gln Val

130 135 140

Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu

145 150 155 160

513

Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu Thr Phe Leu Pro Ser Arg

165 170 175

Phe Pro Trp Gly Pro Pro Pro Leu His Trp Asn Ser Ala Tyr Leu Gly

180 185 190

Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr His Arg Trp Ile Val Thr

195 200 205

Ser Met Cys Gln Ser Gln Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser

210 215 220

Ser Ala Leu Met Trp Thr Gln Trp Ser Ser Val Thr Ser Leu Glu Trp

225 230 235 240

Thr Ala Gln Glu Arg Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp

245 250 255

Met Lys Ser Trp Ser Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu

260 265 270

Tyr Val Ser Thr Arg Val Leu Trp Met Pro Thr Ser Thr Val Gly Ala

275 280 285

Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln Thr Pro

290 295 300

His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro Arg Ala

305 310 315 320

Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys

325 330 335

Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Ser Trp Pro Pro Ala Gly Ala

340 345 350

Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly

355 360 365

Arg Met Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys

370 375 380

Phe Thr Gly Val Arg Thr Ser Ser Arg His Pro Ser Trp Pro Trp Thr

385 390 395 400

Arg Cys Leu Arg Met Arg Pro Pro Arg Ser Asn Ser Arg Lys Lys Leu

405 410 415

Leu Glu Asp Met Lys Gln Asn Gly Phe Thr Arg Tyr Lys Lys Arg Gly

420 425 430

Val Ile Ala Trp Arg Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys

435 440 445

514

Ser Leu Pro Gln Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu

450 455 460

Leu Val Gln Ser Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys

465 470 475 480

Ser Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys

485 490 495

Lys Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys

500 505 510

Gly Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Leu Ile Leu Ile

515 520 525

Gln His Gly Gly Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu Ser

530 535 540

Trp Asn Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val Gln

545 550 555 560

Arg Leu Leu Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu Asn

565 570 575

Leu Phe His Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu

580 585 590

Lys Tyr Thr Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln

595 600 605

Gln Gln Cys Tyr Gln Leu Leu Leu Ala His Met Tyr Gly Phe Ser Thr

610 615 620

Tyr Gly Thr Gln Lys Gly Glu Cys Val Cys Leu Gln Cys Leu Ser Gly

625 630 635 640

Ser Ser Leu Phe Ile Leu Lys Gln Pro Leu Asp Leu Lys Ile Ser Lys

645 650 655

Thr Phe Met Ser Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys

660 665 670

Lys Val Lys Ala Ser Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn

675 680 685

Phe Glu Thr Ile Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn

690 695 700

Leu Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys

705 710 715 720

Ile Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val His Val Leu Met

725 730 735

515

Phe Val Leu Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly

740 745 750

Ile Tyr Pro Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Ile Arg Met

755 760 765

Cys Ala Leu Ser His Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe

770 775 780

Leu Ser Lys His Cys Met Thr Ala Cys Thr Arg Glu Lys Ile Ile Asn

785 790 795 800

Pro Thr Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Gly His

805 810 815

Arg Glu Gly Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro

820 825 830

Pro Trp Leu Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe

835 840 845

Gln Trp Gly Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Asn

850 855 860

Ser Val Ile Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser

865 870 875 880

Ser Tyr Asn Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile

885 890 895

Glu Phe Ala Val Leu Lys Leu Leu Asn Met Asp Leu Ala Ser Pro Gln

900 905 910

Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg Met

915 920 925

Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly Ala

930 935 940

Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Ile Leu Phe His

945 950 955 960

Val Arg Asn Ile Val Tyr Gly Ile Gln Val Met Leu Cys Arg Arg Arg

965 970 975

Asn Ser Arg Lys Gln Ser Gln Val Gln Leu Ala Ile Gln Val Leu Leu

980 985 990

Val Asp Lys His Lys Tyr Phe Leu Phe Gly Pro Tyr Pro Ile Ser Glu

995 1000 1005

Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu

1010 1015 1020

Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His Ala

516

1025 1030 1035

Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser

1040 1045 1050

Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys

1055 1060 1065

Lys Phe Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Gln Lys

1070 1075 1080

Lys Gly His Met Ile Phe Ser Pro Lys Phe Phe Leu Thr Phe Ser

1085 1090 1095

Gln Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr

1100 1105 1110

Gln Cys Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn

1115 1120 1125

Ile His His Arg Lys Asn Pro Leu Leu Pro Met Arg Met Ser Arg

1130 1135 1140

Thr Pro Pro Arg Arg Val Cys Gly Ser Pro Pro Leu His Leu Cys

1145 1150 1155

Gln Pro Leu Arg Gly Gly Lys Lys Arg Ser Lys Phe Ala Phe Trp

1160 1165 1170

Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr Gly Cys

1175 1180 1185

Trp Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly

1190 1195 1200

Thr Pro Arg Gly Asp Trp Lys Arg His Pro Pro Arg Arg Glu Leu

1205 1210 1215

Ser Leu Glu Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln

1220 1225 1230

Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile

1235 1240 1245

Gln Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe

1250 1255 1260

Glu Ala Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile

1265 1270 1275

Thr Leu Phe Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val

1280 1285 1290

Leu Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys

1295 1300 1305

517

Arg Lys Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln

1310 1315 1320

Arg Leu Phe Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His

1325 1330 1335

Leu Gln Asn Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr

1340 1345 1350

Ile Asn Ile Leu Thr Arg Ala Phe Ile His Thr Pro Arg Ser Pro

1355 1360 1365

Gly Cys Ser Val Tyr Ala Asn Met Cys Trp Arg Lys Met Gln Ser

1370 1375 1380

Leu Asn His Pro Ser Arg Leu Thr Asn Cys His Pro Ala Trp Leu

1385 1390 1395

Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His

1400 1405 1410

Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro

1415 1420 1425

Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp

1430 1435 1440

Lys Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser Cys Leu Glu

1445 1450 1455

Lys Lys Ala Thr Trp Met Arg Arg Thr Pro Gly Thr Ser Leu Gln

1460 1465 1470

Cys Tyr Leu Asn Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly

1475 1480 1485

Ile Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile

1490 1495 1500

<210> 1157

<211> 1506

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 3C polypeptide

<400> 1157

Pro Ile Pro Pro His Leu Arg His Pro Leu Leu Leu Lys Asn Lys Lys

1 5 10 15

Arg Arg Lys Leu Arg Pro Lys Ser Arg Lys Lys Gly His Thr Lys Asn

20 25 30

Arg Arg Lys Ala Lys Arg Lys Pro Gln Ile Arg Asn Ser Arg Arg Ala

518

35 40 45

Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Gly

50 55 60

Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr Thr

65 70 75 80

Pro Ser Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln Ser

85 90 95

Lys Lys Thr Met Thr Ala Ala Gly Leu Thr Pro Ser Gln Lys Lys Asp

100 105 110

Pro Ala Gly Lys Leu Lys Lys Gln Val Ser Val Tyr Ser Gly Asn Leu

115 120 125

Leu Ser Phe Ser Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn

130 135 140

Cys Thr Asn Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys

145 150 155 160

Arg Val Phe Ile Leu Leu Leu Ser Asp Lys Lys Ser Ile Pro His Val

165 170 175

Pro Lys Ala Thr Ala Ala His Ser Val Thr Val Lys Leu Gln Ser Arg

180 185 190

Arg Asn Arg Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu

195 200 205

Gln Ala Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr Pro Arg

210 215 220

Leu Ser Val Ser Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys

225 230 235 240

Gly Leu Asn Trp Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met

245 250 255

Leu Lys Lys Lys Tyr Leu Val Gln Arg Glu Trp Glu Lys Arg Lys Gln

260 265 270

Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu Lys Arg Ile

275 280 285

Leu Ile Arg Lys Ala Met Thr Val Leu Ala Gly Asp Lys Lys Gly Leu

290 295 300

Gly Arg Phe Met Arg Cys Val Gln Ser Glu Thr Lys Ala Val Ser Leu

305 310 315 320

Gln Leu Pro Leu Gly Arg Met Asp Leu Asp Gly Met Leu Thr Val Ile

325 330 335

519

Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu Gly Thr Val Ala

340 345 350

Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln Val Gln Thr Gly Gly

355 360 365

Arg Leu Arg Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro

370 375 380

Ala Asn Gln Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg

385 390 395 400

Thr Glu Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His

405 410 415

Asp Leu Pro Arg Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln

420 425 430

Met Pro Phe Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp

435 440 445

Glu Thr Pro Ser Thr Trp Gly Trp Arg Thr Phe Ala Arg Gln Pro Ser

450 455 460

Arg Pro Thr Gly Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly

465 470 475 480

Ser Val Met Ser Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala

485 490 495

Cys Thr Met Pro Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala

500 505 510

Gly Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser Met

515 520 525

Thr Thr Ile Ser Arg Ala Thr Trp Arg Arg Asn Leu Gln Arg Asn Leu

530 535 540

Lys Val Leu Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln Gln

545 550 555 560

Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys Gln

565 570 575

Ser Gln Arg Arg Gly Lys Leu Ser Leu Thr Thr Leu Ser Phe Val Leu

580 585 590

Ile Glu Glu Leu Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg

595 600 605

Asn Val Ser Arg Ile Ser Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln

610 615 620

520

Arg Ser Phe Leu Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln Met

625 630 635 640

Thr Asn Val Gly Cys Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly

645 650 655

Asp Arg Val Phe Leu Val Lys Ile Gln Ser Ser Pro Phe Leu Ala Arg

660 665 670

Ser Trp Asn Thr Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val

675 680 685

Ile Ala Gly Ala His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val

690 695 700

Trp Lys Met Asn Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu

705 710 715 720

Phe Ser Pro Leu Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His

725 730 735

Gly Lys Arg Cys Asn Pro Cys Cys Arg Leu Thr Trp Met Ser His Leu

740 745 750

Asn Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr Met

755 760 765

Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile Pro Leu

770 775 780

Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His Pro Pro

785 790 795 800

Pro Pro Cys Leu Leu Leu Leu His His Arg His His Leu Ala Phe Leu

805 810 815

Glu Met Ala Cys Ser Ser Gln His Thr His Ser Ile Lys Ile Trp Ile

820 825 830

Ser Phe Ser His Glu Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe

835 840 845

Arg Leu Thr Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg

850 855 860

Ala Arg Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Ser Leu Phe Phe

865 870 875 880

Ser His Cys Leu Cys Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp

885 890 895

Ile Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val

900 905 910

521

Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala

915 920 925

Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr Met Val

930 935 940

Met Lys Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu

945 950 955 960

Thr Lys Arg Glu Gly Pro Glu Thr Gly Pro Val Tyr Tyr Val Cys Asn

965 970 975

Arg Met Asn Val Ser Ser Val Leu Lys Pro Phe Leu Phe Thr Val Pro

980 985 990

Ile Glu Asp Met Arg Glu Cys Thr Leu Gly Arg Asn Arg Met Asn Val

995 1000 1005

Ser Ser Val Leu Lys Pro Cys Leu Ile Pro Val Pro Ile Cys Lys

1010 1015 1020

Lys Lys Leu Leu Leu Lys Leu Leu Arg Gly Ala Ser Ser Ser Ser Thr

1025 1030 1035

Ser Arg Asp Ser Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg

1040 1045 1050

Pro Cys Arg Met Arg Leu Tyr Gln Met Leu Gln Lys Gln Cys Cys

1055 1060 1065

Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu

1070 1075 1080

Arg Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly

1085 1090 1095

Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys

1100 1105 1110

Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Lys

1115 1120 1125

Gly Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn

1130 1135 1140

Gln Gly His Leu Leu Met Ile Leu Leu His Asn Arg Gly Pro Pro

1145 1150 1155

Leu His Thr Arg Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser Arg

1160 1165 1170

Lys Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro Ala Phe Ser

1175 1180 1185

Gly Arg Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met

522

1190 1195 1200

Pro Lys Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr Val His Lys

1205 1210 1215

Lys Arg Gly Leu Phe His Gln Met Lys Cys Leu Thr Ala Val Asp

1220 1225 1230

Cys Leu Gln Ala Phe Trp Asp Met Ser Glu Lys Lys Arg Lys Leu

1235 1240 1245

Pro Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala Asn Arg

1250 1255 1260

Asn Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala His Leu Lys Lys

1265 1270 1275

Thr Ser Ala Lys Gly Ala Lys Lys Ile Thr Phe Leu Cys Leu Arg

1280 1285 1290

Val Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Trp

1295 1300 1305

Leu Lys Lys Ser Thr Lys Ser Arg Leu Cys Phe Phe Tyr Leu Leu

1310 1315 1320

Tyr Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Thr Pro Pro Pro

1325 1330 1335

Leu Ile Leu Trp Pro Gln Ala Ala Pro Gly Glu Lys Leu Lys His

1340 1345 1350

Cys Phe Lys Ala Trp Gly Val Asp Pro Pro Gly Ser Val Trp Ile

1355 1360 1365

Leu Pro Cys Phe Leu Ile His Leu Arg Lys Val Asp Thr His His

1370 1375 1380

Leu Gln Val Val Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly

1385 1390 1395

Gln Arg Met Arg Arg Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp

1400 1405 1410

Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser His Phe

1415 1420 1425

Pro Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln Leu

1430 1435 1440

Arg Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val

1445 1450 1455

Ala Met Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu

1460 1465 1470

523

Ser Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro

1475 1480 1485

Val Thr Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro

1490 1495 1500

Gln Thr Ala

1505

<210> 1158

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 4C polypeptide

<400> 1158

Asp Ser Lys Lys Met Thr Gln Lys Asn Arg Lys Thr Arg Lys Ala Ala

1 5 10 15

Arg Glu Lys His Arg Lys Thr Asp Asn Leu Asp Leu Gln His Gln Arg

20 25 30

Arg Arg Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro

35 40 45

Lys Ala Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn

50 55 60

Leu Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys

65 70 75 80

Asp Glu Glu Asp His Gln Lys Arg His His His His Asn Gln Lys Lys

85 90 95

Met Ser Val Asn Glu Gly Asn Asn His Trp Pro Arg Val Glu Met Pro

100 105 110

Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn

115 120 125

Pro Arg Leu Gln Gln His Leu Lys His Trp Gln Gln Cys Thr Arg Ala

130 135 140

Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe

145 150 155 160

Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly

165 170 175

Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val

180 185 190

Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp Leu Ile Cys Phe Val

524

195 200 205

Phe Phe Val Cys Val Glu Thr Gly Phe Cys His Val Ala Gln Val Gly

210 215 220

Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp

225 230 235 240

Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro

245 250 255

Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala Arg Leu Trp Leu Lys

260 265 270

Asn Trp Lys Arg Phe Lys Leu Lys Lys Lys Tyr Thr Gly Met Glu Ile

275 280 285

Leu Leu Trp Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys Ile Cys

290 295 300

Leu Gly Glu Met Gln Leu Cys Leu Lys Lys Lys Val Asn Ala Cys Ser

305 310 315 320

Asn Ala Lys Lys Met Pro Phe Gln Cys Arg Cys Arg Arg Glu Tyr Arg

325 330 335

Val Thr Met Ile Gln Lys Lys Lys Gln Lys Ser Pro Leu Phe Phe His

340 345 350

Gly Asp Leu Cys Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile

355 360 365

Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp Ser

370 375 380

Ser Lys Met Ala Lys Lys Ser Gln Gln Pro Leu Cys Arg Gly Gly Pro

385 390 395 400

Ser Ala Ser Asp Arg Cys Cys Ser Cys Ser Pro Ser Gly Phe Ser Ala

405 410 415

Gly Arg Gly Arg Cys Pro Val Gln Gly Cys Leu Arg Pro His Arg Val

420 425 430

Gln Leu Leu Arg Arg Trp Gly Pro Gly Ser Pro Ala Gly Gln Arg Leu

435 440 445

Ser Lys Gly Phe Gln Leu Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala

450 455 460

Pro Glu Pro Arg Lys Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro

465 470 475 480

Val Thr Ala Val Thr Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser

485 490 495

525

Val Asp Ala Leu Glu Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser

500 505 510

Ser Pro Arg Asn Leu Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly

515 520 525

Ile Phe Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn

530 535 540

Lys Ser Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln Ile Thr His

545 550 555 560

Pro Phe Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln

565 570 575

Ile Arg Leu Leu Lys Pro Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp

580 585 590

Phe Ser Ala Pro Gln Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly

595 600 605

Thr Ser Gln Thr Tyr Phe Gly Ser Ile Glu Ala Leu Thr Ala Leu Phe

610 615 620

Thr Cys Arg Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met Ser

625 630 635 640

Ile Leu Glu Ala Ile Leu Ala Met Thr Leu Thr Leu Asn Gly Ser Pro

645 650 655

Leu Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu Trp

660 665 670

Glu Ile Pro Ser Val Thr Leu Leu Ala Thr Ala Lys Lys Ser Pro Thr

675 680 685

Ser Phe Cys Cys Gln Arg Pro Pro Leu Gln Pro Val Glu Glu Lys Lys

690 695 700

Asn Gly Arg Ile Ser Ser Val Pro Pro Ser Pro Pro Leu Ala Leu Gly

705 710 715 720

Pro Arg Met Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr

725 730 735

Pro Pro Val Trp His Ile Leu Gly Pro Gln Arg His Thr Pro Leu Leu

740 745 750

Thr Pro Asp Thr Arg Trp Ile Phe Ser Asn Gln Leu Gln Thr Val Gln

755 760 765

Met Leu Cys Leu Pro Pro Leu Lys Gln Gly Glu Arg Ile Ile Trp Pro

770 775 780

526

Leu Gly Gly Phe Gln Ile Pro Asn Phe Phe Trp Ser Leu Gly Ile Gly

785 790 795 800

His Thr Gly Ala Trp Trp His Thr Gly Arg Arg Arg Arg Glu Trp Gly

805 810 815

Gly Ser Thr Val Ser Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr

820 825 830

Leu Arg Gly Met Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg

835 840 845

Val Ser Trp Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser

850 855 860

Asp Lys Lys Lys Lys Ser Thr Glu Lys Thr Lys Asn Lys Ile Ile Cys

865 870 875 880

Lys Gly His Arg Ile Thr Gln Val Val Leu Arg Leu Gly Asn Leu Pro

885 890 895

Pro Phe Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr

900 905 910

Lys Cys Gln Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu His Pro

915 920 925

Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly Ser Arg

930 935 940

Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr Lys Trp

945 950 955 960

Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr Leu Val

965 970 975

Trp Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala His Cys Pro

980 985 990

Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser Ser Cys

995 1000 1005

Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala Val

1010 1015 1020

Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile Gln

1025 1030 1035

Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser Trp

1040 1045 1050

Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys Arg

1055 1060 1065

527

Trp Pro Arg Arg Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu

1070 1075 1080

Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu Ile Ser

1085 1090 1095

Ser Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro Ser

1100 1105 1110

Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp Thr

1115 1120 1125

Trp Ser Ser Trp Leu Lys Glu Trp Gly Gly Gln Ala His Arg Gln

1130 1135 1140

Arg Asn Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg Arg

1145 1150 1155

Met Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr Val

1160 1165 1170

Arg Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp Ser

1175 1180 1185

Leu Ile Leu Ser Phe Gln Arg Val Lys Thr Ser Arg Ile Leu Asn

1190 1195 1200

Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys Leu Tyr

1205 1210 1215

His Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu Glu Lys

1220 1225 1230

Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Gly

1235 1240 1245

Gln Ala Phe Gly Ser Leu Cys Gln Leu Val Ser Ala Asp Phe Ser

1250 1255 1260

Ala Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His Leu Leu Ile

1265 1270 1275

Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met Leu Cys Leu

1280 1285 1290

Pro Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val His Val Leu

1295 1300 1305

Lys Lys Asn Arg Lys Gln Ile Ile Cys Ile Lys Asn Met Phe Leu

1310 1315 1320

Gln Arg Lys Gly Phe Leu Asn Leu Ser His Val Glu Lys Phe Arg

1325 1330 1335

Glu Ser Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro

528

1340 1345 1350

Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly

1355 1360 1365

Arg Ala Val Arg Arg Lys Gly Val Val Ile Asn Leu Lys Arg Leu

1370 1375 1380

Gln Lys Phe Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr

1385 1390 1395

Thr Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp

1400 1405 1410

Pro Pro Arg Ser Gln Lys Ser Gln His His Cys Ala Leu Arg Lys

1415 1420 1425

Ile Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro

1430 1435 1440

Gln Ala Gln Glu Lys Ala Asn Ile Leu Asn Ser Leu Pro Ser Ser

1445 1450 1455

Met Glu Ile Ala Val Leu Gln Glu Met Leu Asn Gln Lys Cys Leu

1460 1465 1470

Ala Pro Phe Glu Leu Lys Lys Asn Leu Leu Leu Pro Thr Tyr Thr

1475 1480 1485

Pro His Ile Cys Arg His Thr Leu Lys Lys Lys Pro Arg Asp Ile

1490 1495 1500

Cys Arg Ile Ser

1505

<210> 1159

<211> 1507

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 1D polypeptide

<400> 1159

Ala Asp Lys Lys Asn Val Arg Ser Ala Ser Ser Pro Gly His Pro Leu

1 5 10 15

Gly Pro Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met Met

20 25 30

Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met Thr

35 40 45

Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro

50 55 60

529

Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln

65 70 75 80

Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp Gly

85 90 95

Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile Thr Pro Leu

100 105 110

Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro

115 120 125

Gly Leu Thr His Pro Ala His Gln Pro Leu Gly Ser Met Val Gly Ala

130 135 140

Gly Thr Gly Gly Gly Pro Ala Cys Val Pro Val Ala Glu Ala Ser Thr

145 150 155 160

Gly Ala Trp Thr Ala Glu Gly Ala Ala Gly Thr Arg Cys Arg Ala Ser

165 170 175

Arg Ala Pro Cys Arg Gln Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu

180 185 190

Ile Cys Val Lys Arg Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg

195 200 205

Ser Pro Val Pro Lys Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala

210 215 220

Arg Thr Ala Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly

225 230 235 240

Met Leu Leu Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala

245 250 255

Arg Arg Glu Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg

260 265 270

Pro Pro His Leu Pro Ser Cys His Pro Leu Pro Gln Leu Leu Gly Pro

275 280 285

Pro Pro Pro His Gln Ala Pro Gly Glu Lys Asn Asp Leu Gln Leu Phe

290 295 300

Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu

305 310 315 320

Asn Pro Cys Gly Asn Leu His Leu Lys Thr Thr Ser Leu Asn Ala Lys

325 330 335

Lys Met Glu Asp Met Ile Trp Glu Ser Asn Gln Gln Leu Lys Trp Thr

340 345 350

Pro His Ile Leu Lys Ser Ala Ser Ile Ala Lys Lys Arg Ile Lys Leu

530

355 360 365

Arg Arg Asn Ser Thr Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser

370 375 380

Pro Pro Gly Pro Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser

385 390 395 400

Gln Ser Lys Leu Arg Leu Arg Asn Thr Ser Pro Phe Asp Lys Lys Gly

405 410 415

Phe Val Ile Leu Ser Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr

420 425 430

Ile Val Phe Phe Leu Ala Pro Tyr Phe Pro Met Pro Ser Ser Trp Ser

435 440 445

Ser Ser Gly Arg Thr Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg

450 455 460

Pro Leu His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg

465 470 475 480

Gly Asp Cys Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro

485 490 495

Ala Ser Gly Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala

500 505 510

Cys Arg Ala Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Arg Lys Arg

515 520 525

Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu Gln Leu

530 535 540

Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg Ser Pro

545 550 555 560

Trp Ile Phe Glu Ala Lys Lys Ser Met Lys Arg Ser Gln Arg Pro Pro

565 570 575

Arg Ser Ile Leu Ser Leu Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser

580 585 590

Pro Ser Val Ser Lys Lys Arg Leu Pro Lys Leu Lys Gly Lys Lys Arg

595 600 605

Arg Lys Glu Ile Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val

610 615 620

Pro Leu Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser

625 630 635 640

Cys Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met Arg

645 650 655

531

Ser Leu Leu Tyr Ser Arg Met Cys Leu Val Gln Ile Pro Thr His Thr

660 665 670

Trp Glu Pro Asp Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala

675 680 685

Thr Gln Ser Phe Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile

690 695 700

Pro Cys Cys Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Thr

705 710 715 720

Leu Ala Ile Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val

725 730 735

Thr Ala Ala Pro Ser Gly Gln Tyr Phe Ala Leu Arg Ser Met Pro Lys

740 745 750

Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu

755 760 765

Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln

770 775 780

Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln Ser Met Leu Val Arg Thr

785 790 795 800

Arg Leu Thr Leu Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro

805 810 815

Ser Ala Val Cys Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro

820 825 830

Ala Ala Ser Pro Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro

835 840 845

Pro Glu Val Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala

850 855 860

Cys Ala Gly Leu Trp Pro Trp Gly Ala Gln Val Arg Met Lys Lys Lys

865 870 875 880

Asp Ser Thr Ile Phe Ile Tyr Pro Arg Leu Lys Thr Phe Gly Val Pro

885 890 895

Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr Met Met Ile

900 905 910

Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser Val Pro Cys

915 920 925

Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala

930 935 940

532

Met Pro Ser Ala Met Gly Val Ala Leu Pro Thr Ser Cys Glu Ser Gly

945 950 955 960

Arg Ser Pro Pro Ala Thr Gly Gly Arg Met Pro Gly Ser Gln Ser Ile

965 970 975

Met Met Ser Trp Met Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys

980 985 990

Ser Pro Ala Pro Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro Thr

995 1000 1005

Ala Val Pro Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro Val

1010 1015 1020

Leu Ser Gly Cys His Leu Ala Val Arg Thr Ser Met Leu Thr Leu

1025 1030 1035

Leu Pro Met Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln

1040 1045 1050

Phe Arg Asn Tyr Pro Arg Arg His Pro His Arg Ser Pro Pro Thr

1055 1060 1065

Ser Cys Pro Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro Arg Ala Ser

1070 1075 1080

Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala Thr Gly

1085 1090 1095

Leu His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe Leu

1100 1105 1110

Leu His Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser His Arg

1115 1120 1125

Leu Arg Leu Arg Leu Pro Arg Thr Thr Glu Ile Gln Leu Thr Met

1130 1135 1140

Asn Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val

1145 1150 1155

Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp

1160 1165 1170

Ser Arg Gln Val Leu Ala Val Pro Met Ser Leu Ala Cys Ser Ile

1175 1180 1185

Thr Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val

1190 1195 1200

Ser Ser Ile Ser His Trp Asn Gly Ile Trp Glu Val Met Pro Arg

1205 1210 1215

533

Thr Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr Ser Gln

1220 1225 1230

Asn His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu Asn

1235 1240 1245

Thr Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile

1250 1255 1260

Thr Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His

1265 1270 1275

Pro His Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe

1280 1285 1290

Ser His His Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg

1295 1300 1305

Glu Arg Gly Gly Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Ala

1310 1315 1320

Ser Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly

1325 1330 1335

Met Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg

1340 1345 1350

Cys Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly

1355 1360 1365

Trp His Arg Ser Gly Leu Arg Ala Gln Thr Ala Phe Phe Leu Leu

1370 1375 1380

Asp Ile Ile Leu Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys

1385 1390 1395

Arg Met Trp Trp Arg Leu Pro Cys Lys Val Thr Glu Ser Ser Leu

1400 1405 1410

Ala Lys Lys Thr Leu Thr Asn Gln Pro Ser Leu Pro Gly Pro Cys

1415 1420 1425

Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro

1430 1435 1440

Ser Gln Val Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro

1445 1450 1455

Ala Ala Ala Cys Pro Pro Leu Lys Leu Ala Pro Leu Gln Val Thr

1460 1465 1470

Thr Thr Leu Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu

1475 1480 1485

Asn Lys Val Arg Gln Asn Phe Lys Arg Asn Ser Ser Phe Phe Tyr

534

1490 1495 1500

Thr Ser Asp Arg

1505

<210> 1160

<211> 1501

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 2D polypeptide

<400> 1160

Arg Lys Met Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Asn Pro

1 5 10 15

Arg Arg Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser

20 25 30

Gln Lys Asn His Leu Tyr Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg

35 40 45

Lys Lys Ser Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys

50 55 60

Val Lys Arg Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu

65 70 75 80

Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro

85 90 95

Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Gln Lys Lys Gly His Met

100 105 110

Ile Phe Ser Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn Leu

115 120 125

Ile Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Ala Ser Pro

130 135 140

Gln Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg

145 150 155 160

Met Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly

165 170 175

Leu Leu Leu Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys

180 185 190

Gly Glu Cys Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile

195 200 205

Leu Lys Gln Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Ala Ser

210 215 220

535

Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Leu

225 230 235 240

Gln Ala His Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro

245 250 255

Ala Arg Arg Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu

260 265 270

Thr Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln

275 280 285

Cys Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr

290 295 300

Ala Asn Met Cys Trp Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg

305 310 315 320

Ile Tyr Pro Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Leu Ile Leu

325 330 335

Ile Gln His Gly Gly Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu

340 345 350

Ser Trp Asn Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val

355 360 365

Gln Arg Leu Leu Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu

370 375 380

Asn Leu Phe His Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr

385 390 395 400

Leu Lys Tyr Thr Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile

405 410 415

Gln Gln Gln Cys Tyr Gln Arg Lys Arg Met Lys Thr Gly Ala Ile Gln

420 425 430

Glu Lys Gly Ala Ile Gln Glu Lys Gly Ala Met Thr Lys Arg Ile Cys

435 440 445

Val Val Asn Phe Glu Ala Cys Arg Glu Met Glu Ser Leu Ser Ala Pro

450 455 460

Glu Lys Ile Thr Leu Phe Glu Ala His Met Ala Arg Cys Thr Ser Ile

465 470 475 480

Asn Val Leu Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu

485 490 495

Lys Arg Lys Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln

500 505 510

Arg Leu Phe Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His Leu

536

515 520 525

Gln Asn Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn

530 535 540

Ile Leu Thr Phe Leu Pro Ser Arg Phe Pro Trp Gly Pro Pro Pro Leu

545 550 555 560

Gly Ala Leu Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln

565 570 575

Thr Pro His Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro

580 585 590

Arg Ala Glu Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg

595 600 605

Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu Ser Ser Trp Pro Pro Ala

610 615 620

Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln

625 630 635 640

Arg Gly Arg Met Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr

645 650 655

Arg Cys Phe Thr Gly Val Arg Thr Ser Ser Arg Cys Ser Leu Met Glu

660 665 670

Gln Ile Pro His Leu Lys Ser Asn Ser Arg Lys Lys Leu Leu Glu Asp

675 680 685

Met Lys Gln Asn Gly Phe Thr Arg Arg Arg Asn Ser Arg Lys Gln Ser

690 695 700

Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp Lys His Lys Tyr

705 710 715 720

Phe Leu Phe Gly Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser

725 730 735

Leu Pro Gln Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu

740 745 750

Val Gln Ser Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met

755 760 765

Arg Pro Pro Arg Ser His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu

770 775 780

Leu Val Thr Cys Ile Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys

785 790 795 800

Gln Ser Gln Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu

805 810 815

537

Met Trp Thr Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys

820 825 830

Met Cys Lys Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro

835 840 845

Trp Lys Gly Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Met Ser

850 855 860

Arg Thr Pro Pro Arg Arg Val Cys Gly His Arg Glu Gly Ser Glu Ser

865 870 875 880

Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Val Leu Phe Lys Ala

885 890 895

Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly Asn Ser Val Ile Val His

900 905 910

Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu

915 920 925

Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu

930 935 940

Lys Leu Leu Asn Met Asp Leu Gly Lys Gly Leu Thr His Pro Val Pro

945 950 955 960

Leu Lys Arg Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val

965 970 975

Asn Gln Lys Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu

980 985 990

Ile Val Leu Asn Ser Pro Glu Ser Asn Arg Glu Lys Ile Ile Asn Pro

995 1000 1005

Thr Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Gly Ser

1010 1015 1020

Pro Pro Leu His Leu Cys Gln Pro Leu Arg Pro Tyr Pro Ile Ser

1025 1030 1035

Glu Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser

1040 1045 1050

Leu Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His

1055 1060 1065

Ala Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe

1070 1075 1080

Ser Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu

1085 1090 1095

538

Cys Lys Phe Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly Ala

1100 1105 1110

Phe Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr

1115 1120 1125

Gly Cys Trp Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala

1130 1135 1140

Gln Glu Arg Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met

1145 1150 1155

Lys Ser Trp Ser Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu

1160 1165 1170

Tyr Val Ser Thr Arg Val Leu Trp Met Pro Thr Ser Thr Val Pro

1175 1180 1185

Lys Phe Phe Leu Thr Phe Ser Gln Ile Arg Met Cys Ala Leu Ser

1190 1195 1200

His Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser Lys

1205 1210 1215

His Cys Met Thr Ala Cys Thr Pro Gly Thr Ser Leu Gln Cys Tyr

1220 1225 1230

Leu Asn Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Arg Asn

1235 1240 1245

Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile His His Arg

1250 1255 1260

Lys Asn Pro Leu Leu Pro Met Arg Leu Glu Lys Lys Ala Thr Trp

1265 1270 1275

Met Arg Arg Thr Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr Leu

1280 1285 1290

His Gln Gln Lys Thr Gln Trp Leu Pro Met Arg Ser Leu Gly Glu

1295 1300 1305

Pro Trp Arg Ala Gly Phe Gln Trp Gly Glu Ser Pro Trp Gln Val

1310 1315 1320

Met Ala Ser Leu Thr Gly Arg Arg Lys Lys Arg Glu Asp His Cys

1325 1330 1335

Gly Leu Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser Cys Ala

1340 1345 1350

Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Gly Ser Cys

1355 1360 1365

539

Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr Pro Arg Gly

1370 1375 1380

Asp Trp Lys Arg His Pro Pro Arg Arg Ile Leu Phe His Val Arg

1385 1390 1395

Asn Ile Val Tyr Gly Ile Gln Val Met Leu Cys Cys His Pro Ala

1400 1405 1410

Trp Leu Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro

1415 1420 1425

Phe His Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala

1430 1435 1440

Met Pro Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys Gly

1445 1450 1455

Trp Asp Lys Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro

1460 1465 1470

Leu Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val

1475 1480 1485

His Val Leu Met Phe Gly Gly Lys Lys Arg Ser Lys Phe

1490 1495 1500

<210> 1161

<211> 1506

<212> PROTEIN

<213> Artificial sequence

<220>

<223> Assembled poly-FSP 3D polypeptide

<400> 1161

Gln Asn Thr Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys

1 5 10 15

Gln Ser Gln Arg Arg Gly Lys Leu Ser Ser Arg Lys Lys Gly His Thr

20 25 30

Lys Asn Arg Arg Lys Ala Lys Arg Lys Pro Gln Ile Ser Gln Lys Lys

35 40 45

Asp Pro Ala Gly Lys Leu Lys Lys His Leu Asn Arg Lys Leu His Leu

50 55 60

Thr Arg Ser Cys Leu Ile Asp Leu Thr Met Met Ser Gln Lys Leu

65 70 75 80

Trp Lys Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro Arg Gln His

85 90 95

Leu Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro Cys Leu Leu Leu

100 105 110

540

Leu His His Arg His His Leu Ala Phe Leu Glu Met Ala Cys Ser Ser

115 120 125

Gln His Thr His Ser Ile Lys Ile Trp Ile Ser Phe Ser His Glu Asp

130 135 140

Leu Pro Arg Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln Met

145 150 155 160

Pro Phe Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp Glu

165 170 175

Thr Pro Ser Thr Trp Gly Trp Arg Thr Phe Gly Pro Val Tyr Tyr Val

180 185 190

Cys Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Phe Leu Phe Thr

195 200 205

Val Pro Ile Glu Asp Met Arg Glu Cys Thr Leu Gly Arg Asn Arg Met

210 215 220

Asn Val Ser Ser Val Leu Lys Pro Cys Leu Ile Pro Val Pro Ile Leu

225 230 235 240

Thr Thr Leu Ser Phe Val Leu Ile Glu Glu Ser Arg Arg Asn Arg Arg

245 250 255

Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Leu Pro

260 265 270

Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His Thr Thr Thr Thr

275 280 285

Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg Gly Gly Thr Thr

290 295 300

Ile Gly Glu Val Thr Ser Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp

305 310 315 320

Ser Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met

325 330 335

Arg Leu Tyr Gln Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr

340 345 350

Pro Arg Leu Ser Arg Gly Pro Pro Leu His Thr Arg Thr Met Ser Leu

355 360 365

Ser Asn Ile Leu Met Lys Phe Arg Leu Met Leu Gln Lys Gln Cys Cys

370 375 380

Tyr Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg

385 390 395 400

541

Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly Phe Leu

405 410 415

Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys Leu Leu Met

420 425 430

Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Thr Pro Pro Pro Leu

435 440 445

Ile Leu Trp Pro Gln Ala Ala Pro Val His Lys Lys Arg Gly Leu Phe

450 455 460

Ser Leu Phe Phe Ser His Cys Leu Cys His Gln Met Lys Cys Leu Thr

465 470 475 480

Ala Val Asp Cys Leu Gln Ala Phe Trp Asp Met Met Ile Arg Lys Ile

485 490 495

Gln Lys Arg Arg Arg Thr Ala Asn Arg Leu Lys Lys Lys Tyr Leu Val

500 505 510

Gln Cys Thr Asn Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp

515 520 525

Lys Arg Val Phe Ile Leu Leu Leu Ser Asp Lys Lys Cys Leu Arg Val

530 535 540

Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Lys Asn Lys

545 550 555 560

Lys Arg Arg Lys Leu Arg Pro Lys His Leu Lys Lys Thr Ser Ala Lys

565 570 575

Val Ser Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu

580 585 590

Asn Trp Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met Trp Leu

595 600 605

Lys Lys Ser Thr Lys Ser Arg Gln Ser Ser Pro Phe Leu Ala Arg Ser

610 615 620

Trp Asn Thr Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile

625 630 635 640

Ala Gly Ala His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp

645 650 655

Lys Met Asn Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe

660 665 670

Ser Pro Leu Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly

675 680 685

Lys Arg Cys Asn Pro Cys Cys Arg Leu Thr Trp Met Ser Arg Glu Trp

542

690 695 700

Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu Tyr

705 710 715 720

Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val Leu Ala Gly

725 730 735

Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln Ser Glu Thr

740 745 750

Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg Arg Lys Ser Gly Val

755 760 765

Met Lys Cys Trp Asp Leu Cys Pro Arg Leu Asp Phe His Pro Arg Ser

770 775 780

Ser Ser Gly Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly Leu

785 790 795 800

Cys Phe Phe Tyr Leu Leu Tyr Phe Glu Lys Lys Arg Pro Glu Trp Leu

805 810 815

Ser Val Asp Pro Pro Gly Ser Val Trp Ile Leu Pro Cys Met Asp Leu

820 825 830

Asp Gly Met Leu Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro

835 840 845

Arg Asp Leu Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro

850 855 860

Ile Gln Val Gln Thr Gly Gly Arg Leu Phe Leu Ile His Leu Arg Lys

865 870 875 880

Val Asp Thr His His Leu Gln Val Val Thr Lys Thr Val Thr Ala Thr

885 890 895

Thr Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys Lys Ala Cys Arg

900 905 910

Leu Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser

915 920 925

His Phe Pro Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln

930 935 940

Leu Arg Pro Ile Pro His Leu Arg His Pro Leu Leu Leu Lys Gly

945 950 955 960

Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln Gly

965 970 975

His Leu Leu Met Ile Leu Leu His Asn Ala Arg Gln Pro Ser Arg Pro

980 985 990

543

Thr Gly Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val

995 1000 1005

Met Ser Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys

1010 1015 1020

Thr Met Pro Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala

1025 1030 1035

Gly Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser

1040 1045 1050

Met Thr Thr Ile Ser Arg Ala Thr Trp Arg Leu Arg Ile Pro Ser

1055 1060 1065

Leu Arg Lys Tyr Arg Gln Asn Ile Glu Ile Thr Pro Gln Arg Ser

1070 1075 1080

Lys Ala Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser

1085 1090 1095

Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Ser Ile Pro

1100 1105 1110

His Val Pro Lys Ala Thr Ala Ala His Ser Val Thr Val Lys Leu

1115 1120 1125

Gln Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Asp Arg Val Phe

1130 1135 1140

Leu Val Lys Ile Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu Arg

1145 1150 1155

Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro Ala Asn

1160 1165 1170

Gln Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg Thr

1175 1180 1185

Glu Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val

1190 1195 1200

Ala Met Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu

1205 1210 1215

Ser Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro

1220 1225 1230

Val Thr Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro

1235 1240 1245

Gln Thr Ala Ala Phe Ser Gly Arg Ile Leu His Gln Pro Glu Met

1250 1255 1260

544

Ala Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr Asn Ser

1265 1270 1275

Trp Trp Thr Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile

1280 1285 1290

Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val

1295 1300 1305

Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly

1310 1315 1320

Ala Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr

1325 1330 1335

Met Val Met Lys Gly Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro

1340 1345 1350

Ser Pro Arg Glu Thr Thr Pro Ser Ser Asp Ala Thr Arg Arg Thr

1355 1360 1365

Arg Pro Ile Ala Ala Gln Ser Lys Lys Thr Met Thr Ala Ala Gly

1370 1375 1380

Leu Thr Pro Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu Gly

1385 1390 1395

Ala Lys Lys Ile Thr Phe Leu Arg Asn Ser Arg Arg Ala Thr Pro

1400 1405 1410

Thr Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Asn Ile

1415 1420 1425

Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe

1430 1435 1440

Pro Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys Leu

1445 1450 1455

Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser

1460 1465 1470

Arg Ile Ser Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu

1475 1480 1485

Ser Leu Thr Lys Arg Glu Gly Pro Glu Thr Ser Glu Lys Lys Arg

1490 1495 1500

Lys Leu Pro

1505

<210> 1162

<211> 1507

<212> PROTEIN

<213> Artificial sequence

545

<220>

<223> Assembled poly-FSP 4D polypeptide

<400> 1162

Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp

1 5 10 15

Asp Lys Lys Lys Lys Ser Thr Glu Lys Thr Lys Asn Ala Lys Lys Met

20 25 30

Pro Phe Gln Gln Arg Pro Pro Leu Gln Pro Val Asn Leu Asp Leu Gln

35 40 45

His Gln Arg Arg Arg Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn

50 55 60

Arg Ser Pro Lys Ala Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg

65 70 75 80

Glu Gln Asn Leu Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His

85 90 95

His Arg Lys Asp Glu Glu Asp His Gln Lys Arg His His His His Asn

100 105 110

Gln Lys Lys Met Ser Val Arg Ala Ser Val Ser Phe Trp Thr Leu Leu

115 120 125

Pro Thr Thr Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu

130 135 140

Trp Pro Pro Arg Val Pro Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg

145 150 155 160

Met Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro

165 170 175

Val Trp His Ile Leu Gly Pro Gln Arg His Thr Pro Gln Ala Pro Leu

180 185 190

Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser Arg

195 200 205

Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro Gly His

210 215 220

Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp Ser Ser Ser Lys Arg Trp Ile

225 230 235 240

Phe Ser Asn Gln Leu Thr Val Gln Met Leu Cys Leu Pro Ile Thr His

245 250 255

Pro Phe Ala Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln

260 265 270

546

Ile Arg Leu Leu Lys Pro Cys Arg Val Asn Ala Val Glu Glu Leu His

275 280 285

Ser Arg Arg Met Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys

290 295 300

Tyr Val Arg Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp

305 310 315 320

Glu Glu Lys Lys Asn Gly Arg Ile Ser Ser Leu Lys Lys Lys Val Asn

325 330 335

Ala Cys Ser Ser Leu Ile Leu Ser Phe Gln Arg Val Lys Thr Gly Met

340 345 350

Glu Ile Leu Leu Trp Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys

355 360 365

Ile Cys Leu Gly Glu Met Gln Leu Cys Gly Gln Ala Phe Gly Ser Phe

370 375 380

Ser Ala Asp Phe Ser Ala Pro Gln Ile Ile Lys Lys Met Lys Lys Arg

385 390 395 400

Ile Met Val His Glu Leu Lys Lys Asn Leu Leu Leu Pro Met Cys Ser

405 410 415

Ser Leu Ala Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu Pro Ser Ser

420 425 430

Asp Leu Ala Val Leu Ile Ser Ser Ser Thr Ser His Phe Leu Met Arg

435 440 445

Ser Pro Val Leu Pro Ser Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu

450 455 460

Pro Arg Met Trp Thr Trp Ser Ser Ser Trp Leu Lys Asn Glu Gly Asn Asn

465 470 475 480

His Trp Pro Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr

485 490 495

Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys

500 505 510

His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln

515 520 525

Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu

530 535 540

Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys

545 550 555 560

547

Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln

565 570 575

His Val Trp Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly

580 585 590

Phe Cys His Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser

595 600 605

Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser

610 615 620

Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly

625 630 635 640

Val Trp Ala Arg Leu Ile Leu Ala Met Thr Leu Thr Leu Asn Gly Met

645 650 655

Ala Lys Lys Ser Gln Gln Pro Leu Gln Val Val Leu Arg Leu Gly Asn

660 665 670

Leu Pro Pro Phe Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys

675 680 685

Arg Thr Lys Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu

690 695 700

Val Gly Arg Ala Val Arg Arg Lys Gly Ser Pro Leu Trp Asp Cys Ser

705 710 715 720

Thr Thr Thr Gly Thr His His Gly Cys Leu Trp Glu Ile Pro Ser Val

725 730 735

Thr Leu Leu Ala His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr

740 745 750

Ser Leu Lys Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg

755 760 765

Leu Pro Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly

770 775 780

Thr Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg Ser

785 790 795 800

Leu Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His His Cys Arg

805 810 815

Thr Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln

820 825 830

Trp Ser Gly Ala Val Val Gly Leu Arg Pro Val Ala His His Leu Pro

835 840 845

Arg Pro Ile Gln Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala

548

850 855 860

Leu Ser Trp Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys

865 870 875 880

Cys Arg Trp Pro Arg Arg Cys Gln Lys Lys Val Pro Ser Ala Ser Pro

885 890 895

Trp Gly Leu Val Leu Lys Lys Asn Arg Lys Gln Met Leu Asn Gln Lys

900 905 910

Cys Leu Ala Pro Phe Arg Glu Ser Thr Leu Gly Asn Phe Trp Met Lys

915 920 925

Leu Leu Gln Leu Pro Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe

930 935 940

Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser

945 950 955 960

Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln Ser Gln Lys Ser Gln

965 970 975

His His Cys Ala Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser Leu

980 985 990

Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys Ala Thr Ser Arg Ile

995 1000 1005

Leu Asn Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys

1010 1015 1020

Leu Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu

1025 1030 1035

Glu Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr

1040 1045 1050

Lys Pro Leu Phe Phe His Gly Asp Leu Cys Glu Trp Gly Gly Gln

1055 1060 1065

Ala His Arg Gln Arg Asn Trp Leu Lys Asn Trp Lys Arg Phe Lys

1070 1075 1080

Leu Lys Lys Lys Tyr Ile Gln Lys Lys Lys Gln Lys Ser Asn Lys

1085 1090 1095

Ile Ile Cys Lys Gly His Arg Ile Thr Ile Ile Cys Ile Lys Asn

1100 1105 1110

Met Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu Ser His Val Glu

1115 1120 1125

Lys Phe Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser

1130 1135 1140

549

Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val

1145 1150 1155

Gln Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp

1160 1165 1170

Gly Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln

1175 1180 1185

Leu Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg

1190 1195 1200

Lys Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala

1205 1210 1215

Val Thr Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp

1220 1225 1230

Ala Leu Glu Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser

1235 1240 1245

Pro Arg Asn Leu Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly

1250 1255 1260

Ile Phe Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys Cys Asn Ile

1265 1270 1275

Leu Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu

1280 1285 1290

Asp Ser Lys Lys Met Thr Gln Lys Gly Gln Ala Phe Gly Ser Leu

1295 1300 1305

Cys Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr Arg

1310 1315 1320

Leu Arg Ser Leu Asn His Leu Leu Ile Thr Tyr Thr Pro His Ile

1325 1330 1335

Cys Arg His Thr Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser

1340 1345 1350

Asn Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys

1355 1360 1365

Gln Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro

1370 1375 1380

Asn Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp

1385 1390 1395

His Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser

1400 1405 1410

550

Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met

1415 1420 1425

Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp

1430 1435 1440

Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Ile Glu

1445 1450 1455

Ala Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile Ser Val

1460 1465 1470

Asn Ile Ser Thr Leu Met Ser Ile Leu Glu Ala Leu Lys Lys Lys

1475 1480 1485

Pro Arg Asp Ile Cys Arg Ile Ser Cys Arg Cys Arg Arg Glu Tyr

1490 1495 1500

Arg Val Thr Met

1505

<210> 1163

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 1C

<400> 1163

agcaccagac gggaaatgcc ttctcaggcc acaccttctc cacctggacc ttggagagcc 60

gctctgggca aagaagtgaa gcggagccag agcaagctgc ggctgagaaa cacaagcccc 120

accgagatcc aattgaccat gaacgacagc aagcacaagc tggaaagccc cgctctgaaa 180

caggtgtccc ctgcctctcc acctacacag cagcctcaga caccccagga tagcagacag 240

gtgctggccg cttcttctcc tggacatcct cttggccctg gcgactgccc tagaacaatg 300

gctggacctt ctgcctctct gcacccagca tctggcagag ccagcagaaa gacctggcag 360

agaaggctga gagcctgtag agctggtgga cctgctggat gggctagaca ggccatgaca 420

ctgaaggccc tggtcaagac ctggacacag ttcagaaact accccagagt cggagccgga 480

acaggcggag gacctgcttg tgttcctgtg gccgaagctt ctactggcgc ctggacagct 540

gaaggcgctg ccggaacaag atgcagagct tctagagccc cttgcagaca gctgcctgcc 600

agctctccta ccaagtgcga gatctgcgtg aagagaaggc ggcggagaaa gcggagcagc 660

cagtctagat ctcccgtgcc taagaaacgc gagcggatga tgctgcctgt gcgggccaga 720

acagctagat ctgctctgcc acaaggcaga gcccctctgg aaggaatgct tctgcctaga 780

gcactgccca gcctgctgag cagaatgcca gccagaagag agagccggtt ttggctggca 840

ctgcaggctc tgggactcag acctcctcat ctgcctagct gtcaccctct gcctcaactg 900

cttggacctc ctccacctca tcaggcccct ttcgacaaga aaggcttcgt gatcctgagc 960

aagggcaaga agcgccggaa agaaatccgg cggatgacca gacggaccat gaccaccacc 1020

1080 aaaatgatga tgatgcggcg gaccaagcgg cggaggcgaa

atgacccgca gaaccctgct gaccaagaaa acctgtctga cccctagaac cagcgccgcc 1140

acaaaagctc tgccgaca gacaatcagc cagcgggaggc ctatccagac acctcctgga 1200

acaagcctgg ccatctgggg cagactgcct ctcagaccac agagaggacc tcggcctctg 1260

catagatggc tgcagcagac aacaggctgc tcccagagaa gaatcgccaa gaagcggatc 1320

aagctgaggc ggaacacact ggccatccgg tttatctctg cccctcagcc tagcagcagc 1380

accgttacag ctgctcctag cggccagtac ttcagcagct tcttctacac cagcgaccgg 1440

accaagtgtg aaatctgtgt caagaacacc gccatcctgg ctatcaccct gatcacccct 1500

caggaccctc acctggaccc tgtgtgtcac ccacatagaa ggcctggctg gaagggccac 1560

cacagagtgg tgtttagcca ccatccaagc ggctccacca agagccattg gcagcagaga 1620

gaaagaggcg gaggccgcag agaaaaaaag ggctgcagag agatgcccag cagctggtct 1680

agctctggca gaaccgccga caaaaagaac gtgcggagca gaaccagact gaccctgaca 1740

ggcacaagaa gcagcggcac aagagcctct ccaagcgccg tgtgtctgtg ctgttggaca 1800

tgcccctcca gaagcttccc tgccgcatct ccatctggac tgagagcacc tgttggagcc 1860

caggctcctc ctgaagtgac actgagagcc agctgtgcct ctagcgcctc taaagcctgt 1920

gctggacttt ggccttgggg agcccaagtg cggaacaaag tgcggcagaa cttcaagcgg 1980

aacctggcca aaaagaccct gactaacgcc ctgcggagta tgcccaagag cagaaaccag 2040

gtgctgttca gacggaatct gaccccaagc cagctgagaa ctctggcccc tccttacatg 2100

ctgctgcccc agtctctgtc tcagagcctg tccagaaagc agatccccag ccaatcaatg 2160

ctcgtgaccg ccttcttcct gctcgacatc atcctgagaa gcctgctgta ctcccggatg 2220

tgcctggtgc agatccctac acacacctgg gagcccgact acttcgtgtc cctgcctatg 2280

atgttcagct gggccactca gagctttcca gtgggagccg tgttcctgac cagacctgcc 2340

attccttgct gcagctgtac cagccacagc aacccttgga gccagtcctt caagaagaag 2400

aagcccctga cattcagccc cagcgtgcca atgtctctgg cctgtagcat caccgtgtcc 2460

ttcgtgggcc agatcagcac actggtgctg gacgtgtcct ccatctctca ctggcgcaag 2520

cgcaagagag agaagcggtc ccagaagtcc cggcagaacc atctgcagct gaagtcctgc 2580

agacggaaga tcagcaactg gtccaagctg agatccccgt ggatcttcta ccctcggctg 2640

aaaaccttcg gcgtgccact gggctctatc ctgtgtctgg caggctccct gagcacgatg 2700

atgatcatta gcaccaccag acaagagtgt ggcagacggg cctccgtgcc ttgtagacct 2760

atgatcggca gcgctagacc cggaccttgg cggacatctg ctatgccatc tgccatggga 2820

gtcgccctgc ctaccagctg tgaaagcgga agatctcctc ctgccaccgg cggaagaatg 2880

cctggatctc agtctatcat gatgagctgg atgcctccac tggctccctg tgccgcttgt 2940

ccttgttctc ctgctcctac actgtgccct gctcatcctg caagagcccc tacagccgtg 3000

cctgccttta cacctctgtc tgctcaccct gtgccagtgc tgtctggatg tcacctggcc 3060

gtgcgcacct ctatgctgac actgctgccc atgagacacc ctcacagaag cccacctaca 3120

agctgccctc cactgctgga agctttccca ctgccaaggg ccagcctgag agctaccgtg 3180

cctagacaca gctctgtgct ggccactgga ctgcatctgc atcggcagat tagcctgcct 3240

ctgaggggct tcctgctgca tcaacctctg cctggacaac acctgtggct gtcccacaga 3300

ctgaggctga gactgccaag aacaggcgag aagaacgacc tccagctgtt cgtgatgagc 3360

gacaccacat acaagatcta ctggacagtg atcctgctga acccctgcgg caacctgcac 3420

ctgaaaacca catctctgcg gagagccaga cctacctcct gcagcacatg cagaatgtgg 3480

tggctgaagc tggccccact gcaagtgacc acaacactga agctgatcct ggtcatgctg 3540

gaaatcgtga ccgaagaggc caagaaatcc atgaagagat cccggcggag caagatcgag 3600

gcctctccta tcggctccat catgaagaag aaagacagca ccatcttcat caaccagcag 3660

ctcaagtgga cccctcacat cctgaagtct gccagcgtgt ccaagaagag actgcccaag 3720

ctgaacggca tctgggaagt gatgccccgg acactggaag gctgcatcag cgtgaaggac 3780

caccggacaa gccagaacca cagcaagtgc atcaccgagt gtgtgcggac agccctgaac 3840

cagagaaagg tggtcaagaa ccccagccag ccagtgccac tgagaaacga cggcatcacc 3900

aacggcaacg actccaccag atcctgctcc atcagcctga agaaaagcct ggaaaagatg 3960

cccctgatga acgccaagaa aatggaagat atgatctggg agagcaacat gggcggcaac 4020

cggtccatct gcctgacaag actgccctgc aaagtgaccg agtccagcca aagaccacct 4080

cggagcattc tgtccctggc ctctagagcc ggacagtttc ccgtttggcg gagaaccagc 4140

agaggcatgt ccctgcagtg gaagtccggc aatgcctgca cacctctgag gtgccctagc 4200

agaagccctg ccacatttct gaccgcactg aacggctggc acagatctgg acttagggct 4260

cagcaaccta gcctgccagg accttgtgct agcctgctgt ctacactgag ccagcctcca 4320

ccacaggctc caagccaagt ttggacagcc gccacactga gatgtcccgc tgttcctgca 4380

gctgcttgcc ctcctatcgt gttctttctg gccccatact tccccacacc actgagcatc 4440

agcagaccca cctctcgggc cagacagtct tgttgtctcc ccggactgac acaccctgct 4500

caccagcctc ttggctctat g 4521

<210> 1164

<211> 4503

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 2C

<400> 1164

cggcagatca acagacggaa gctgcagaga aagaagcgcc ggaagaagag agaggaccac 60

tgcggactgc gggccagaaa cgggaacatg aagaaaacca tgagccacct gatcgcccat 120

caggccctga agatgtctgg cctgagactg tttcacccca gactgctgac acggaagaag 180

cctggcaaga accctcggag aaagacctgg aagatgcgga agaagtacgc ccagaagcct 240

agccagaaga accacctgta cggcaagggc ctgacacacc ctgtgcctct gaagagaacc 300

cgcgtgaacc agaagaaggg caacatcaag 360

agaaagagca tcctgctgca cctcatcgtg ctgaacagcc ccgagagcaa cctgcaggct 420

catcctcaag ttccagcact ggctcctcag gcttggtggc ctgctagaag aggccctctg 480

acaagcgctc catctgctca gcagagcctg accttcctgc ctagcagatt tccttggggc 540

cctcctccac tgcactggaa ctctgcctac ctgggcagaa cactgctcgt gacctgcatc 600

ctgtaccaca gatggatcgt gaccagcatg tgccagagcc agagatggct gaccagcacc 660

acctctagaa gcagcgccct gatgtggacc cagtggtcta gcgtgacaag cctggaatgg 720

acagcccaag agaggggctg tagcagctgg ctgatgaagc agacctggat gaagtcttgg 780

agcctgcggg accccagcta cagatccatc ctggaatacg tgtccaccag agtgctgtgg 840

atgcccacat ctacagtggg agccctgaga gccacagctc ctgcaacagc tgctccacag 900

gctcagaccc ctcattggag agccgccaca acaagcagca tgagcagcag ccctagagcc 960

gagcctacaa gctctagctc ttgggccacc tgttggccca gatgttgcag ctccagctgt 1020

tactggtccc tgtcctcttg gcctcctgct ggtgctgccg aggccacaaa taccaggacc 1080

agaagcagag agtcccagcg gggcagaatg ctgacttgga gaagcagcct gtggctgcag 1140

ggcaccagat gttttacagg cgtgcggacc tccagcagac acccttcttg gccttggacc 1200

agatgcctga gaatgcggcc tcctagaagc aacagccgga aaaagctgct cgaggacatg 1260

aagcaaaatg gcttcacccg gtacaagaaa cggggcgtga tagcctggcg gagagccact 1320

ttcctgctgg ccctttggga gtgcagtctg ccccaagcta gactgtgcct gatcgtgtct 1380

aggaccctgc tgctggtcca gagcagatgc agcctgatgg aacagatccc tcacctgaag 1440

1500

ggctgcaccg cctgtgttga aagatgtcct tggaagggca tctgctggaa gtggcctgtg 1560

acagtggccg ctctgatcct gattcagcac ggcggcatct tcaccttccg gttctgggtc 1620

gagaagctgt cctggaacat ctgcaagaat ctgtccctgt acctggacaa gttcgtgcag 1680

cggctgctgt acgtgtacat catcgagaca aacgtgttcg tcctgaacct gttccacgtg 1740

ccagtgaatc agatcttcat gatggccttc tacctgaagt acaccgagaa ggcctgggtc 1800

atggaactgg aatggctcat tcagcagcag tgctaccagt tgctgctggc tcatatgtac 1860

ggcttcagca cctacggcac ccagaaaggc gagtgcgtgt gtctgcagtg tctgagcggc 1920

agctccctgt tcatcctgaa gcagccactg gacctgaaga tcagcaagac cttcatgagc 1980

agatcccacc tggatttcag cccctattgg atgaagaaag tgaaggcctc tccacagatc 2040

atgcagcgga tgagcgccgt gaacttcgag acaatcaggc ggaagtggca gatccggcac 2100

gtgctgaaga acctgcctct ggaaaacaca cagatcccca ggacaagccc tctgtggaag 2160

atcacaatgg tgctgacata ccccaactgc gtggtgcatg tgctgatgtt cgtgctgttc 2220

aaggccgacc agagcgagtc tagcctgagc agcggaatct accctcctag cacctgtagc 2280

ctgccttgcc ggggcattag aatgtgcgcc ctgtctcacc ctagcttcta catcagcagc 2340

accgtgtgct ttctgagcaa gcactgcatg accgcctgca ccagagagaa gatcatcaac 2400

cccaccatca gctgcccctt tcagagcccc acaaagagac tgggccacag aggggcagc 2460

gagagcgata atctgggcct gctgtactcc tttcctcctt ggctgcccat gagatccctg 2520

ggcgaacctt ggagagctgg ctttcagtgg ggagagtccc cttggcaagt gatggctagc 2580

ctgaccggca acagcgtgat cgtgcacaag atcgtgctgg tgtttctgaa gtctctgagc 2640

agctacaact gcgagcggcg gatctctggc atccagaaga gagccatcga gttcgccgtc 2700

ctgaagctgc tgaacatgga cctggctagc cctcagatta tgcagagaac cagcgccgac 2760

cggttccgga tgaagcggag aatgcagaac ctggataacc tgggcgtgcc ctggccattg 2820

cttccaggtg ctgctcctcc acatagactg ctgggcccct ctagatggat tctgtttcat 2880

gtgcggaaca tcgtgtacgg catccaagtg atgctgtgcc ggcggagaaa cagcagaaag 2940

cagagtcagg tgcagctggc cattcaggtc ctgctcgtgg acaagcacaa gtactttctg 3000

ttcggcccct atcctatcag cgagggactg accatgacca ccggccggaa catcaacagc 3060

aactccctga tgaccaactg cctgttcagc cccatcttcc tgtgccacca cgccatcatg 3120

caccggaaga tgacaagccc acacttcaga ctgttctcca gcaagatccc acatcctcag 3180

gtgccaagtg tggtggccct gtgcaagttc ctggccaatg gcctgtggtt caacctgatc 3240

tttcagaaaa agggccacat gatctttagc cccaagttct tcctgacctt tagccagcgg 3300

accctggaat ccgccggact gtttccatgg ctgcacaccc agtgccggaa caaaaagacc 3360

aagctgtggt tttccctgat caacatccac caccgcaaga atcctctgct gcctatgaga 3420

atgagcagaa cacctcctcg gagagtgtgc ggatctccac ctctgcatct gtgccaacct 3480

ctgagaggcg gcaagaagag aagcaagttc gccttctggga agcctcctag cccagggcatc 3540

agctcttgtc tgatgacagg ctgttggggc tcctgtggca gagcttgttc ttgcggaaga 3600

3660

gaaccatgga ttccttatct gcaccagcaa aagacccagc ggaagcggat gaagaccggc 3720

gccattcaag agaagggcgc tatccaagaa aaaggcgcaa tgaccaagcg gatctgcgtg 3780

gtcaatttcg aggcctgcag agagatggaa agcctgagcg cccctgagaa gattaccctg 3840

ttcgaagccc acatggcccg gtgcacctcc atcaatgtgc tgtgtgtccg ggccagcctg 3900

attgagaagc tgatgaaaga 3960

atcatgcaac ggctgttcat caagggctac cagaacaacc acttcacgtt ccacctccaa 4020

aactttgcct tcttcagctc cttcggcaac aacaagtata tcaacattct cacccgggcc 4080

ttcatccaca cacctagaag ccctggctgt tccgtgtacg ccaatatgtg ctggcggaag 4140

atgcagtccc tgaatcaccc cagcagactg accaactgtc accccgcttg gctgagcccc 4200

attagtgctt gtcctaggct gctgccctat ccttttcacg tgctggcact ggaagtgacc 4260

tttccacctg gcacagccat gcctcagatg caattgcagg cactggctat gtgggagaaa 4320

ggctgggaca agcaagagaa gaaatcccgc aagctgatgt cctcctgcct ggaaaaaaag 4380

gctacctgga tgcggagaac ccctggcaca agcctgcagt gttacctgaa cacacacccc 4440

tgcaccatgc tgctgcctca gggaatccca tctctgaacg cccagtggat gttcctgcgg 4500

atc 4503

<210> 1165

<211> 4518

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 3C

<400> 1165

cctattcctc cacacctgag acaccctctg ctgctgaaga acaagaagcg gagaaagctg 60

cggcccaaga gcagaaagaa gggccacacc aagaaccggc ggaaggccaa gagaaagccc 120

cagatcagaa acagccgcag agccacacct accagcaagg gcagcctgag aagaaagtac 180

ctgagagtgg gagccgccgc tgctagctgt caatctcctc catctcctag agagacaacc 240

cctagcagcg acgccaccag aagaaccaga cctatcgccg ctcagagcaa gaaaaccatg 300

acagccgccg gactgacccc tagccagaag aaagatcctg ccggcaagct gaagaaacag 360

gtgtccgtgt acagcggcaa cctgctgagc ttctctcgga gaggcatgaa gtctagcgtg 420

cgcctgaaga actgcaccaa cctgagcgtg cccatgatgc tgaccatcct gatctggaag 480

cgggtgttca tcctgctgct gtccgacaag aaatctatcc ctcacgtgcc caaggccacc 540

gccgctcatt ctgtgacagt gaagctgcag agccggcgga acagacggta cagaaagatc 600

tggaagcctg tggcacacac 660

cccacaccta gactgtccgt gtccgtgatc accatgaagc tgtgggagaa gcacttcaag 720

ggcctgaact ggaacctggt cgtgtggatt ctgaacctga agatgatgct caagaagaag 780

tatctggtcc agcgcgagtg ggagaaacgg aagcagattc ttggcggcgg aggcaagtac 840

aaagagtact tcctgaagag aatcctcatc cgcaaggcca tgacagtgct ggccggcgat 900

aagaaaggcc tgggcagatt catgagatgc gtgcagagcg agacaaaggc cgtcagtctg 960

cagctgcctc tgggcagaat ggacctggat ggaatgctga ccgtgatcgg ccagggactc 1020

gtgggcatga cacctagaga tctgggaaca gtggctggcg ccaccgcaca gattcagagg 1080

cctattcagg tgcagacagg cggcagactg agaggcccta gaagaaaggt gctgatggtg 1140

ctgtggctgc ccgccaatca gagcctgcag aaaagccagg tgttccagtg ggtgctgaga 1200

accgagctgc ccctgacctt tagcaccctg ctgtgggaca gcagacacga cctgcctaga 1260

atgctgccca tcagcagccc tctgcacaga ccccagatgc ctttctggac ctgcaccttt 1320

cctgccacca catggggcac atgggagaca ccttctacat ggggctggcg gacctttgcc 1380

agacagcctt ctagacccac cggcagaacc cctaccacca gactgatggc tccagtgggc 1440

agcgtgatga gctgtagcct gctgacacag ccatctccag ccagcgcctg tacaatgcct 1500

ccactgctgc atcctcagtg ggcccctcat tctgctggac tttctccagg cgcttgcaga 1560

cctgcctgta cctgcatcag catgaccaca atcagcaggg ccacctggcg cagaaacctg 1620

cagagaaacc tgaaagtgct gcggctgaga atccccagcc tgcggaagta cagacagcag 1680

aacacccggt ccaccaacct cgagaagaac ccatctctga agaagcagag ccagaggcgg 1740

ggcaagctgt ctctgaccac actgagcttc gtgctgatcg aggaactgct gaaaaagagc 1800

agccagctgc ggagaagctg gcggaacgtg tccagaatca gcaacatccg gaagctgatt 1860

tggaccaagc agcggagctt cctgtctctg ttccccaagc tgagagccac cgagcagatg 1920

accaatgtgg gctgtagact ggattttcac cccagaagca gctccggcga cagagtgttc 1980

ctggtcaaga tccagagcag cccattcctg gccagatcct ggaataccat gcagtggaac 2040

aacaagaaaa tggccatgac tgtgatcgct ggcgcccacc actgtcagta caagctgacc 2100

tacaccaaag tgtggaagat gaattggaag aaaatgatga tcggcagcca gcacctcctg 2160

ttctccccac tggatagctc tccactccag ctgctgctgc acccacacgg caagagatgc 2220

aacccctgtt gtcggctgac atggatgagc cacctgaatc ggaagctgca cctcaccagg 2280

tcctgcctga tcgacctgac catgatgatg agccagaaac tgtggaagaa tcagcggaag 2340

cgcatccctc tgccttctcc aagacagcat ctgctgcctc agtatcctct gcaccctcct 2400

ccaccttgtc tgctgcttct ccaccataga caccacctgg cattcctcga gatggcctgt 2460

agctctcagc acacccacag catcaagatc tggatcagct tcagccacga gacaatgagc 2520

ctgtccaaca tcctgatgaa gttccggctg accaccacca caagcagccc cagaagaagc 2580

cctgctctgc gagctagagg cggcacaaca atcggcgaag tgaccagcag cctgttcttt 2640

agccactgcc tgtgcgtggc ccctagatgt cctagatctg ccgtggacat cagccccgga 2700

cctacatctc cttctctggc ctcttgtgcc tctgtggcca ttggaaagct ggcactgctt 2760

ggcgcctctg ctccttgtgg tgctgctggc gctagagagg ccggaagatt tgctgtgctg 2820

ggcaccatgg tcatgaagaa catggaacac agactgacca tggacagcgc cctgagcctg 2880

acaaagagag agggccctga aacaggcccc gtgtactacg tgtgcaaccg gatgaatgtg 2940

tccagcgtgc tgaagccttt cctgttcacc gtgcctatcg aggacatgag agagtgcacc 3000

ctgggacgca acagaatgaa tgtctcttcc gtgctgaaac cctgtctgat ccccgtgcca 3060

atctgcaaga agaaactgct gctcaagctg ctgaggggcg cctccagctc taccagcaga 3120

gatagcagaa gctttttcac cagcgctcac cccagcagac cctgcaggat gagactgtac 3180

cagatgctgc agaagcagtg ctgctacaac ctcgtgatca agcaccctct gatcttccgg 3240

atcaacctgc ggagacaggg caagaccaaa gacggcgttg tgcagagcct gggcttcctg 3300

aaccagcaga tcatcctcca gaaagagttc ttcatctgcc tgctgatgtt catcaagcgg 3360

tggaagccag ccgccaccaa gaagggcaag aacagcccct gctgccagaa aaaactgcgg 3420

gtgcagaacc agggccatct gctgatgatt ctgctgcaca acagaggccc acctctgcat 3480

accagattcg agaaaaagcg gcccgagtgg ctgagcagaa aatccggcgt gatgaagtgc 3540

tgggatctgt gccctgcctt cagcggcagg attctgcacc agccagaaat ggccgccatg 3600

agactgatgc ccaagttcct gaactccacc aacagctggt ggacagtgca caagaagaga 3660

ggcctgttcc accagatgaa gtgtctgacc gccgtggact gtctgcaggc cttctgggat 3720

atgagcgaga agaaacgcaa gctgcctatg attcgcaaaa tccagaagcg gcggagaacc 3780

gccaaccgga acatcgagat cacccctcag agaagcaaag cccacctgaa gaaaacctcc 3840

gccaagggcg ccaagaaaat caccttcctg tgcctgagag tcagatgcgg aggctggctg 3900

tgtggctgga caccaagagg atggctgaag aagtccacca agtccagact gtgcttcttc 3960

tacctgctgt actacctgtt tttcctgacc gtgtgcggct tcacccctcc accacttatt 4020

ctgtggcctc aggcagcccc tggcgagaag ctgaagcact gttttaaagc ctggggcgtc 4080

gaccctcctg gcagcgtttg gattctgccc tgcttcctga ttcacctgag aaaggtggac 4140

acccaccacc tccaggtggt caccaaaaca gtgaccgcca caacagccga cggccagagg 4200

atgagaaggc tgaagtgcaa ggcctgccgg ctgagatggg ctagacctga acctgcagct 4260

caggcccctc ctaagagcca ctttccacct catcctagcg gcccctccag aaccagctct 4320

ctgcaattga gaacctacat ggaaatgcac tacggccatc aggccccaca ggtggcaatg 4380

ctgcctaaac tgctgtgtcg gcagatgctc cccactctgt ctaccctgca gcagagacat 4440

cctcagggcc catgtcctcc tccagtgaca cctctgagag tgcagtctgt gctgctgctc 4500

ggagtgcctc aaacagct 4518

<210> 1166

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 4C

<400> 1166

gacagcaaga agatgaccca gaagaaccgc aagacccggga aggccgccag agagaagcac 60

agaaagaccg acaacctgga cctgcagcac cagcgcagac gcaaaaagaa gaaggacaag 120

gtcgagatcc ggaatcggag ccctaaggcc aatagcaccg agtgtcaggg cgagcacagc 180

agagagcaga acctgctgaa cctggtgcaa ttgaaccctc acagccctca ccaccggaag 240

gacgaggaag atcaccagaa gagacaccac caccacaatc aaaagaagat gtccgtcaac 300

gagggcaaca accactggcc tagagtggaa atgcctaccg gctggctgct cgtgggctac 360

aacaccagaa acacagccca gaatcctcgg ctgcagcagc acctgaaaca ctggcagcag 420

tgcaccagag ccagatggtg tcaagagccc tgtcagaccc tgaccttctt ccacttcttc 480

ttctttttca aaacggtgtc cctgtgctgc cccggctggt ctaaaggaca acctcctcca 540

cctggcctga agccttctag cctggcttct caggtggcca gaaccacagg cgtgtaccag 600

cacgtgtggc tgatctgctt cgtgtttttc gtgtgcgtgg aaaccggctt ctgtcacgtg 660

gcccaagtgg gactcgagag actcgttagc ctgagcggca tcaccgacgt gaaccattgg 720

gcccagccta agccatctcc tagcaaaggc accggcatga acagacccat cggcctgaga 780

gttggcaaag gcgtgtgggc tagactgtgg ctgaagaact ggaagcggtt caagctcaaa 840

aagaaataca caggcatgga aatcctgctg tggattctgc tgaagatgga aattcagatc 900

ttcaagattt gcctgggcga gatgcagctg tgcctcaaga agaaagtcaa cgcctgcagc 960

aacgccaaga aaatgccctt ccagtgccgg tgcagacgcg agtacagagt gaccatgatt 1020

cagaagaaga aacagaagtc cccgctgttc ttcacggcg acctgtgcag accttctcct 1080

tgcctgtctt ggagcagcag gatcgtggct cctggacacg ccctgagctg tcggactcgt 1140

agaagatggt ccagcaagat ggccaagaag tcccagcagc ctctgtgtag aggcggccct 1200

tctgccagcg atagatgctg tagctgtagc cccagcggct ttagcgctgg aagaggcaga 1260

tgtcctgtgc agggatgtct gaggccacac agagttcagc tgctgagaag atggggccct 1320

ggatctcctg ctggccagag actgtctaag ggcttccaac tgctgcgttg gtggggacca 1380

ggatctccag ctcctgagcc tagaaagggc ccatttccac cacctgatcc tccatggcct 1440

gtgaccgctg tgacagtgat ggctggctct gtgcctagcg ctcagtctgt ggatgctctg 1500

gaatcccctg gacctctggc tctcgaaggc cctagcagcc ctagaaacct gctctggcgg 1560

gaaatgagca tcttcctgcc tggcatcttc aacctgcacc tgtgctacta ccacagccag 1620

agcaaccgga acaagagccg gcagatggaa tccctgggca tgaagctgca gatcacacac 1680

ccattcgcca gccagccact gggccaagag atgcccaaca acacccagat cagactgctg 1740

aagcccggcc aggcctttgg cagcttttcc gccgattttt cagcccctca gcaggcccct 1800

ctgagactgt ggtcttggtg cggcacaagc cagacctact tcggctctat cgaagccctg 1860

acagccctgt tcacctgtag aaccgccagc atcagcgtga acatcagcac cctgatgagc 1920

atcctggaag ccatcctggc catgacactg accctgaatg gcagccctct gtgggactgc 1980

agcacaacaa caggcacaca ccacggctgc ctgtgggaga tcccttctgt taccctgctg 2040

gctaccgcca aaaagagccc taccagcttc tgctgtcagc ggcctcctct gcagccagtg 2100

gaagagaaga agaacggccg gatcagcagc gtgccacctt ctccacctct tgctctgggc 2160

cctagaatgc agctctgcac cggctggcc cggttcttcc ctattacacc tcctgtgtgg 2220

cacatcctgg gacctcagag acacacccct ctgctgaccc ctgacaccag atggatcttc 2280

tccaaccagc tgcagaccgt gcagatgctg tgtctgcctc cactgaagca gggcgagaga 2340

atcatctggc ctctcggcgg ctttcagatc cccaacttct tttggagcct cggcatcgga 2400

cacactggcg cctggtggca tactggaagg cggagaagag aatggggcgg cagcaccgtg 2460

tctagaagcc cactgtggat cagctccatg atctacctga gaggcatggc cttcgcctcc 2520

gacagcagca tcagaaccac cagagtgtcc tggtgtcgga gatgtggcgc caaaagcgcc 2580

gcagccagca gcgacaagaa gaagaaaagc accgaaaaga ccaagaacaa gatcatctgc 2640

aagggccaca gaatcaccca ggtggtgctg agactgggca acctgcctcc attcttcaga 2700

ggcagcgagg tgccctttcc tagaccttgc cggaccaagt gccagaagaa ggttccaagc 2760

gcctctcctt ggggactgca cccaagaaca gcctccatca cactgcctac caccagcctg 2820

aagggcagca gaacaagctg ctgccccaga tctcacccca gactgcccag aacaaagtgg 2880

cctctgcagt gcctgctgca cagcctggga acaactggaa cactcgtgtg ggaaaaggggc 2940

gtgctgcaga tgagaagtct ggaagctcac tgccctctgc agacattcca cctccaccac 3000

tgcagaaccc agcctagctc ttgtcctcca gcctggattc tgtaccaagg acagtggagc 3060

ggagccgtcg tgggacttag acctgtggct caccatctgc caagacctat ccaggggctg 3120

cactgtcctc aggctcctca tcaaccagct ctgtcctggg gaaagtggcc cgtgggaatg 3180

acaggacccg ccagatgttg ccggtggcct agaagaatgt gtagctctct ggccctgcca 3240

accggcctga catctctgat cctgcctagc agtgatctgg ccgtgctgat ctctagctcc 3300

accagccact ttctgatgcg gagcccagtg ctgccaagca gcagactgac atgtgcctct 3360

ccacagctgc ctcggatgtg gacatggtct agctggctga aagagtgggg aggacaggcc 3420

cacagacagc ggaactgtag agtgaacgcc gttgaggaac tgcacagcag gcggatgtct 3480

gtgcggatcg tgaagaattg ggtgtcctgc tgttacgtgc ggctgagtgc agtgggactg 3540

tctacttgga gcgccctgga ttccctgatc ctgtccttcc agagagtgaa aaccagccgg 3600

atactgaacc tccaggtgct gaagaagatc ctgcggagct ttatgaagct gtaccactct 3660

ctcgtgatgt gcctgcggct gcggaccaag ctggaaaaag ctctgagcgc tctgtttatc 3720

tggccccagc acagctacaa aggccaggca ttcggaagcc tgtgccagct ggtgtctgcc 3780

gatttcagcg cccctcaagt gaccagactg cggagcctga accatctgct gattcggtgg 3840

atctttagca atcagctgac agtccaaatg ctctgcctgc cgatcatcaa gaaaatgaag 3900

3960 aaacggatca tggtgcacgt cttgaagaag

atgttcctgc agcggaaggg attcctgaac ctgagccacg tggaaaagtt cagagagagc 4020

accctgggca atttctggat gaagctcctg cagctgccac cagcttgcac ctctaaggcc 4080

tggacaccca gaaagcttgt ggggagagcc gtgcggagaa agggcgtcgt gatcaacctg 4140

aagagactgc agaaattccg ggctagcgtg tccttctgga ctctgctgcc tacaacaggc 4200

gtgcggacaa gaggctctcc actgagactg actctgtggc ctccaagaag ccagaaaagc 4260

cagcaccact gcgccctgag aaagatcgag tgggccacaa agctgtccct cggcggcaaa 4320

gcaggaccac aggctcaaga gaaggccaac atcctgaaca gcctgccttc ctctatggaa 4380

atcgccgtgc tgcaagaaat gctgaaccag aagtgtctgg cccctttcga gcttaagaag 4440

aacctcctgc tgccaaccta cacaccccac atctgtcggc acacccttaa aaagaagccc 4500

agagacatct gcagaatcag c 4521

<210> 1167

<211> 4521

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 1D

<400> 1167

gccgacaaga aaaacgtgcg gagcgctagt tctcctggac atcctcttgg ccccagaaga 60

atgaccagac ggaccatgac caccaccaaa atgatgatga tgcggcggac caagcggcgg 120

agaagaaaaa gacgccggat gaccatgatg accagaagaa ccctgctgac caagaaaacc 180

tgtctgaccc ctagaaccag cgccgccaca aaagctctgc ccagacagac aatcagccag 240

cggaggccta tccagacacc tcctggaaca agcctggcca tctggggcag aagaagcaag 300

atcgaggcct ctcctatcgg cagcatcacc cctctgagca tcagcagacc taccagcaga 360

gccagacaga gctgttgcct gcctggactg acacaccctg ctcatcagcc tctgggctct 420

atggttggag ctggaacagg cggaggacct gcttgtgtgc ctgtggccga agcttctaca 480

ggcgcttgga cagctgaagg cgctgccggc acaagatgta gagccagcag agccccttgc 540

agacagctgc ctgcttctag ccctaccaag tgcgagatct gcgtgaagag aagaaggcgg 600

cgcaagcgga gcagccagtc tagatctccc gtgcctaaga aacgcgagcg gatgatgctc 660

cctgtgcggg ccagaacagc tagatctgct ctgccacaag gcagggctcc tctggaagga 720

atgcttctgc ctagagcact gcccagcctg ctgagcagaa tgcctgccag aagagagagc 780

agattctggc tggccctgca ggcccttgga ctcagacctc ctcatctgcc tagctgtcac 840

cctctgcctc aactgcttgg acctcctcca cctcatcagg cccctggcga gaaaaacgac 900

ctgcagctgt tcgtgatgag cgacaccacc tacaagatct actggaccgt gatcctgctg 960

aacccctgcg gcaacctgca cctgaaaacc accagcctga acgccaagaa aatggaagat 1020

atgatctggg agagcaacca gcagctgaag tggacccctc acatcctgaa gtccgcctct 1080

atcgccaaga agcggatcaa gctgcggcgg aacagcacca gacgggaaat gccttctcag 1140

gccacacctt ctccacctgg accttggaga gccgctctgg gcaaagaagt gaagcggagc 1200

1260

agcaacatgg gcggcaaccg gtctatctgc ctgaccatcg tgttctttct ggccccttac 1320

ttccccatgc ctagcagctg gtctagcagc ggcagaacca gactgcctct gaggccacag 1380

agaggcccta gacctctgca tagatggctg cagcagacca ccggctgctc tcagagaaga 1440

ggcgactgcc caagaaccat ggccggacca tctgcttctc tgcaccctgc atctggcaga 1500

gcctccagaa agacctggca gagaaggctg agagcctgta gagcaggcgg acctgctgga 1560

tgggctagac aggccagaaa acgcaagaga gagaagcggt cccagaagtc cagacagaac 1620

catctgcagc tgaaaagctg cagacggaag atcagcaact ggtctaagct gcggagcccc 1680

tggatcttcg aggccaaaaa gagcatgaag agatcccagc ggcctcctcg gagcatcctg 1740

agcctgttca agaagaagaa gcccctgaca ttcagcccca gcgtgtccaa gaagaggctg 1800

cctaagctga agggcaagaa acgccggaaa gaaatccagc ggaaggtggt caagaacccc 1860

agccagcctg tgccactgag aaatgacggc atcaccaacg gcaacgacag cacccggtct 1920

tgcagcatca gcctgaagaa aagcctggaa aagatgcccc tgatgcggag cctgctgtac 1980

tccagaatgt gcctggtgca gatccccaca cacacctggg agcccgacta ctttgtgtcc 2040

ctgcctatga tgttcagctg ggccacacag agctttccag tgggcgccgt gtttctgacc 2100

agacctgcca ttccttgctg tagctgtacc agccactcca atccttggag ccagtccaca 2160

ctggccatcc ggtttatctc tgcccctcag ccaagcagca gcaccgttac agctgctcct 2220

agcggccagt attttgccct gcggagtatg cccaagagcc ggaaccaggt gctgtttcgg 2280

agaaatctga ccccaagcca gctgcggaca ctggcccctc catatatgct gctgcctcag 2340

tctctgtccc agagcctgtc tcggaagcag atcccatctc agtccatgct cgtgcggacc 2400

aggctgacac tgacaggcac tagaagctcc ggcaccagag cctctccatc tgccgtgtgt 2460

ctgtgctgct ggacatgccc cagcagaagc tttcctgccg cctctccaag cggactgaga 2520

gcacctgttg gagcacaggc tcctccagaa gtgacactga gagccagctg tgccagctcc 2580

gccagcaaag cttgtgcagg actttggcct tggggagccc aagtgcggat gaagaagaag 2640

gacagcacca tcttcatcta ccccaggctg aaaaccttcg gcgtgccact gggcagcatt 2700

ctgtgtctgg ctggaagcct gagcacgatg atgatcatta gcaccaccag acaagagtgc 2760

ggcagacgggg ccagcgtgcc ctgtagacct atgatcggaa gcgccagacc tggaccatgg 2820

cggacaagtg ctatgccttc tgccatggga gtcgccctgc ctaccagctg tgaaagcgga 2880

agatctcctc ctgccaccgg cggaagaatg ccaggatctc agtctatcat gatgagctgg 2940

atgcctccac tggctccctg cgctgcttgt ccttgttctc ctgctcctac actgtgccct 3000

gctcaccctg ctagagcacc aacagccgtg cctgccttta cacccctgtc tgctcatcct 3060

gtgccagtgc tgtctggatg tcacctggct gtgcggacct ccatgcttac cttgctgcca 3120

atgatgaccc tgaaggccct ggtcaagaca tggacccagt tcagaaacta ccccagacgg 3180

caccctcaca gaagcccacc aacaagctgt cctccactgc tcgaggcctt tccactgcca 3240

agagccagcc tgagagctac cgtgcctaga cactctagcg tgctggccac aggactgcat 3300

ctgcataggc agattagcct gcctctgcgg ggcttcctgc tgcatcaacc actgccagga 3360

caacacctgt ggctgtccca cagactgaga ctgcggctgc ctagaacaac cgagatccag 3420

ctgaccatga acgactccaa gcacaagctg gaaagccccg ctctgaaaca ggtgtcccca 3480

gctagtcctc ctacacagca gcctcagaca ccccaggatt ctagacaggt gctggccgtg 3540

ccctatgagcc tggcctgtag catcaccgtg tccttcgtgg gccagatctc taccctggtg 3600

ctggacgtgt ccctccatcag ccactggaac ggcatctggg aagtgatgcc cagaacactg 3660

gaaggctgca tctccgtgaa ggaccaccgg acatcccaga accacagcaa gtgcatcacc 3720

gagtgtgtgc ggacagccct gaacaccaag tgtgaaattt gtgtgaagaa caccgccatc 3780

ctggctatca ccctgatcac cccacaggac cctcacctgg accctgtgtg tcatccccat 3840

agaaggcccg gctggaaggg acaccacaga gtggtgttta gccaccatcc ttccggcagc 3900

accaagtctc actggcagca gagagaaaga ggcgggaggcc gcagagaaaa gaagggctgt 3960

agagaggcaa gcagagccgg ccagtttcct gtgtggcggga gaacatctag aggcatgtcc 4020

ctgcagtgga agtccggcaa tgcctgcaca cctctgagat gtcccagcag atctccagcc 4080

accttcctga ctgccctgaa cggctggcat agatctggac tgagggccca gaccgccttc 4140

tttctgctgg acatcatcct gcggagagcc cggcctacaa gctgcagcac atgtagaatg 4200

tggtggcggc tgccctgcaa agtgaccgaa agctccctgg ccaagaaaac cctgaccaat 4260

cagcctagct tgcccggacc ttgtgctagc ctgctgtcta cactgagcca gccaccacct 4320

caggctccta gccaagtttg gacagccgct acactgcggt gtcctgctgt gcctgctgct 4380

gcatgtccac ctctgaagct ggctccactg caagtgacca caacactgaa gctgatcctg 4440

gtcatgctgg aaatcgtgac cgagaacaaa gttcggcaga acttcaagcg gaactccagc 4500

ttcttctaca ccagcgacag a 4521

<210> 1168

<211> 4503

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 2D

<400> 1168

cggaagatgc agagcctgaa tcaccccagc agactgacca acaatcctcg gagaaagacc 60

tggaagatgc ggaagaagta cgcccagaag cctagccaga agaaccacct gtaccggcag 120

atcaaccggc gcaagctgca gagaaagaag tccagaagcc acctggactt cagcccctac 180

tggatgaaga aagtgaagcg ggccagaaac cggaacatga agaaaaccat gagccacctg 240

atcgcccatc aggccctgaa gatgtctggc ctgagactgt ttcaccccag actgctgacc 300

agaaagaagc ccggcaagca gaaaaagggc cacatgatct tcagccggcg gaagtggcag 360

atccggcacg tgctgaagaa tctgatcccc agcctgaacg cccagtggat gttcctgaga 420

atcgctagcc cacagatcat gcagagaacc agcgccgacc ggttccggat gaagcggaga 480

atgcagaacc tggacaatct gggcgtgccc tggcctctgt tgcctggatt gctgctggcc 540

catatgtacg gcttcagcac ctacggcacc cagaaaggcg agtgcgtgtg tctgcagtgt 600

ctgagcggca gcagcctgtt catcctgaag cagcccctgg acctgaagat ctccaagacc 660

ttcatggcca gccctcagat tatgcagcgg atgagcgccg tgaacttcga gacaatcctg 720

caggcccatc ctcaggtgcc agctcttgct cctcaagctt ggtggcccgc tagaagaggc 780

cctctgacat ctgctccaag cgcacagcag tccctgacac ggacactgga aagcgccgga 840

ctgtttcctt ggctgcacac ccagtgccgg gccttcattc acacacctag aagccctggc 900

tgcagcgtgt acgccaacat gtgctggcgg tacaagaaac ggggagtgat cgcctggcgg 960

atctaccctc ctagcacatg cagcctgcct tgcagaggcc tgatcctgat tcagcacggc 1020

ggcatcttca ccttccggtt ctgggtcgag aagctgagct ggaacatctg caagaacctg 1080

agcctgtacc tggataagtt cgtgcagcgg ctgctgtacg tgtacatcat cgagacaaac 1140

gtgttcgtgc tgaacctgtt ccacgtgcca gtgaaccaga tcttcatgat ggccttctac 1200

ctgaagtaca ccgagaaggc ctgggtcatg gaactggaat ggctgatcca gcagcagtgc 1260

taccagcgga agagaatgaa gaccggcgcc atccaagaga agggcgctat tcaagagaaa 1320

ggggccatga ccaagcggat ctgcgtggtc aatttcgagg cctgcagaga gatggaaagc 1380

ctgagcgccc ctgagaagat caccctgttt gaagcccaca tggcccggtg caccagcatc 1440

aatgtgctgt gtgtcagggc cagcctgatc gagaaactga tgaaagaaaa gcgcaagatg 1500

aagcgcaacc aggtggcatc ccctcaaatc atgcaacggc tgttcatcaa gggctaccag 1560

aacaaccact tcacgttcca cctccaaaac ttcgccttct tcagctcctt cggcaacaac 1620

aagtatatca acatcctgac ctttctgccc agcagattcc cttggggacc tcctccactg 1680

ggagccctta gagctacagc tcctgctaca gctgctcccc aggcacagac acctcattgg 1740

agagccgcca ccacaagcag catgagcagc tctccaagag ccgagcctac cagctctagc 1800

tcttgggcca cttgctggcc tagatgctgc agctccagct gctattggag cctgtctagc 1860

tggcctcctg ctggcgctgc cgaagccaca aataccagaa ccagaagccg cgagagccag 1920

cggggaagaa tgctgacttg gagaagctcc ctgtggctgc agggcaccag atgttttaca 1980

ggcgtgcgga ccagctccag gtgcagcctg atggaacaga tccctcacct gaagtctaac 2040

agccggaaga aactgctcga ggacatgaag cagaacggct tcaccagacg gcggaacagc 2100

agaaagcaga gccaagtgca gctggccatc caggtgctgc tggtggacaa gcacaagtac 2160

tttctgttcg gcagagccac atttctgctg gctctgtggg agtgtagcct gcctcaggct 2220

agactgtgcc tgatcgtgtc tagaaccctg ctgctcgtgc agtctagaca cccaagctgg 2280

ccttggacca gatgcctgag aatgcggcct ccacgcagcc actggaactc tgcctacctg 2340

gggagaacac tgctcgtgac ctgcatcctg taccacagat ggatcgtgac cagcatgtgc 2400

cagagccaga gatggctgac ctccaccacc tctagaagct ctgccctgat gtggacctgg 2460

tggtgtacaa gaggtggcgg caagcggaag aaaaagatgt gcaaggtgtc cggcggctgc 2520

accgcctgtg ttgaaagatg tccttggaag ggcatctgtt ggaagtggcc tgtgacagtg 2580

gccgccatga gcagaacacc tccaagacgc gtgtgcggcc acagagaggg cagcgagtct 2640

gataatctgg gcctgctgta ctcctttcca cctgtgctgt tcaaggccga ccagagcgaa 2700

agctctctga gcagcggcaa cagcgtgatc gtgcacaaga tcgtgctggt gttcctgaag 2760

tccctgagca gctacaactg cgagagaaga atcagcggca tccagaagag agccatcgag 2820

ttcgccgtcc tgaagctgct gaacatggac ctcggcaagg gactgacaca ccccgtgcct 2880

2940

ggcaacatca agaggaagtc catcctgctg catctgattg tgctgaacag ccccgagagc 3000

3060

ctgggaagcc ctcctctgca cctgtgtcag ccctctgaggc cctatcctat cagcgaggggc 3120

ctgaccatga ccaccggcag aaacatcaac agcaactccc tgatgaccaa ctgcctgttc 3180

agccccatct tcctgtgcca ccacgccatc atgcaccgga agatgacaag ccctcacttc 3240

agactgttct ccagcaagat cccacatcca caggtcccaa gtgtggtggc cctgtgcaag 3300

3360

ggcatctcca gctgtctgat gacaggctgt tggcagtggt ccagcgtgac aagcctggaa 3420

tggacagccc aagagagagg ctgttcctcc tggctgatga agcaaacatg gatgaagtct 3480

tggtccctgc gggaccccag ctacagatcc atcctggaat acgtgtccac acgggtgctg 3540

tggatgccca cctctaccgt gcctaagttc tttctgacct tcagccagat ccggatgtgc 3600

gccctgtctc accctagctt ctacatcagc tccaccgtgt gctttctgag caagcactgc 3660

atgaccgcct gcacacctgg aaccagcctg cagtgttacc tgaacctggc caacggcctg 3720

tggttcaacc tgatcttccg gaacaaaaag accaagctgt ggttctccct gattaacatc 3780

caccaccgca agaacccact gctgcccatg cggctggaaa aaaaggccac ttggatgcgg 3840

cggaccgagc tgtctctgga accttggatt ccttacctgc atcagcagaa aacccagtgg 3900

ctccccatga gaagcctggg cgagccttgg cgagctggat ttcagtgggg agagtctccc 3960

tggcaagtga tggcctctct gacaggcaga cgcaagaaaa gagaggatca ctgcggactg 4020

caagagaaaa aaagccggaa gctgatgtcc agctgcgccg ctcctccaca tagactgctg 4080

ggacctagca gatggggaag ctgtgggaga gcctgttctt gtggcagagg ctgtggaacc 4140

cctagaggcg actggaagag acacccacct cggcggattc tgtttcacgt gcggaacatc 4200

gtgtacggaa tccaagtgat gctgtgctgt caccccgcct ggctgtctcc aatcagcgct 4260

tgtcctcgcc tgctgcctta tccattccac gttctggccc tggaagtgac attccctcca 4320

ggcactgcca tgcctcagat gcaattgcag gcactggcaa tgtgggagaa aggctgggac 4380

aagcctctgg aaaacacaca gatccccagg acaagcccac tgtggaagat cacaatggtg 4440

ctgacatacc ccaactgcgt ggtgcacgtc ctgatgttcg gcggcaagaa gcggagcaag 4500

ttc 4503

<210> 1169

<211> 4518

<212> DNA

<213> Artificial sequence

<220>

<223> polynucleotide 3D

<400> 1169

cagaacacca gaagcaccaa cctggaaaag aaccccagcc tgaagaagca gagccagcgg 60

agaggaaagc tgagcagcag aaagaagggc cacaccaaga accggcggaa ggccaagaga 120

aagccccaga tcagccagaa gaaggaccct gccggcaagc tgaagaaaca cctgaaccgg 180

aagctgcacc tgaccagatc ctgcctgatc gacctgacca tgatgatgag ccagaagctg 240

tggaagaacc agcgcaagag aatccctctg ccttctccac ggcagcatct gctgcctcag 300

tatccactgc atcctccacc tccatgcctg ctgctgctcc accacagaca ccatctggcc 360

tttctcgaga tggcctgcag cagccagcac acccacagca tcaagatctg gatcagcttc 420

agccacgagg acctgcctag aatgctgccc atcagcagcc ctctgcacag accccagatg 480

cctttctgga cctgcacctt tcctgccacc acatggggca catgggagac accttctaca 540

tggggctggc ggacattcgg ccctgtgtac tacgtgtgca accggatgaa cgtgtccagc 600

gtgctgaagc ctttcctgtt caccgtgcct atcgaggaca tgagagagtg caccctgggc 660

agaaacagaa tgaatgtgtc ctctgtgctg aaaccctgtc tgatccccgt gccaatcctg 720

accacactga gcttcgtgct gatcgaggaa agccggcgga acagacggta cagaaagatc 780

tacggggagc tgaagatgtc cctgcaggcc ctgcctctga cctttagcac actgctgtgg 840

gacagcagac acaccaccac cacaagcagc cccagaagat cccctgctct gagagctaga 900

ggcggcacca caatcggcga agtgacaagc agaggcgcca gcagctctac cagcagagac 960

agcagaagct ttttcaccag cgctcacccc agcagaccct gcagaatgag actgtaccag 1020

agcaacggca tggaagcctg cggcacacac cctacaccta gactgtctag aggccctcca 1080

ctgcacacca gaaccatgag cctgagcaac atcctgatga agttccggct gatgctgcag 1140

aagcagtgct gctacaacct cgtgatcaag caccctctga tcttccggat caacctgcgg 1200

agacaggca agaccaaaga cggcgttgtg cagagcctgg gcttcctgaa ccagcagatc 1260

atcctgcaga aagagttctt catctgcctg ctgatgttca tcaagcggtg gaagccagcc 1320

gccacaaaga cacctccacc actgattctg tggcctcagg ccgctcctgt gcacaagaag 1380

agaggcctgt tcagcctgtt cttctcccac tgcctgtgcc accagatgaa gtgcctgacc 1440

gccgtggatt gtctgcaggc cttctgggac atgatgattc gcaagatcca gaagcggcgg 1500

agaaccgcca accggctgaa aaagaaatac ctggtgcagt gtaccaacct gagcgtgccc 1560

atgatgctga ccatcctgat ctggaagcgg gtgttcatcc tcctgctgag cgacaagaag 1620

1680

aagcggagaa agctgcggcc caagcacctg aagaaaacca gcgccaaggt gtccgtgatc 1740

accatgaagc tctgggagaa gcacttcaag ggcctgaact ggaacctggt cgtgtggatt 1800

ctgaacctga agatgatgtg gctcaagaag tccaccaaga gcagacagag cagcccattc 1860

ctggctcgga gctggaatac catgcaatgg aacaacaaga aaatggccat gaccgtgatc 1920

gctggcgccc accactgtca gtacaagctg acctacacca aagtgtggaa gatgaattgg 1980

aagaaaatga tgatcggctc ccagcacctc ctgtttagcc cactggattc aagcccactg 2040

cagttgctgc tgcaccctca cggcaagaga tgcaaccctt gctgcagact gacctggatg 2100

2160

ttcctgaagc ggattctcat ccggaaggct atgacagtgc tggccggcga taagaaaggc 2220

ctgggaagat tcatgagatg cgtgcagagc gagacaaagg ccgtgtctct gcagctgcct 2280

ctcggccgaa gaaagtccgg cgtgatgaag tgttgggatc tgtgccccag actggacttt 2340

caccccagat ctagctctgg cggcgagaag ctgaagcact gtttcaaagc ctggggcctg 2400

tgcttcttct acctgctgta cttcgagaag aaacggcccg agtggctgag cgttgaccct 2460

cctggatctg tctggattct gccctgcatg gacctggacg gaatgctgac agtgatcggc 2520

cagggactcg tgggcatgac ccctagagat ctgggaacag tggctggcgc caccgcacag 2580

attcagaggc ctatccaggt gcagacaggc ggcagactgt tcctgattca cctgagaaag 2640

gtggacaccc accatctgca ggtcgtgacc aagaccgtga cagccacaac agctgacggc 2700

cagaggatgc ggagactgaa gtgcaaggcc tgtcggctga gatgggccag acctgaacct 2760

gctgcacagg ctcctccaaa gagccacttt ccacctcatc ctagcggccc ctccagaacc 2820

tctagcctgc agctcagacc cattcctcca cacctgagac acccactgct gctgaaggga 2880

aagaacagcc cctgctgcca aaagaaactg cgggtgcaga accagggcca tctgctgatg 2940

atcctgctgc ataacgccag acagcccagc aggcctaccg gaagaacccc taccacaaga 3000

ctgatggccc ctgtgggcag cgtgatgtct tgtagcctgc tgacccagcc atctcctgcc 3060

agcgcatgta caatgccacc actcctgcat ccacagtggg ccccacatag tgctggactg 3120

tctccaggcg cttgcagacc tgcctgtacc tgcatcagca tgaccaccat ctccagagcc 3180

acatggcggc tgagaatccc aagcctgagg aagtaccggc agaacatcga gatcacccct 3240

cagcggtcta aggcccaggt ttccgtgtac agcggcaatc tgctgagctt ctccagacgg 3300

ggcatgaaga gtagcgtgcg gctgaagaac agcatccctc acgtgccaaa agccaccgcc 3360

gctcactctg tgacagtgaa gctgcagtac ctgtttttcc tgaccgtgtg cggcttcgac 3420

cgggtgttcc tggttaagat ctgcaagaag aagctgctcc tcaagctgct gagaggcccc 3480

agacggaaag tgctgatggt gctttggctg cccgccaacc agagtctgca gaaaagccag 3540

gtgttccagt gggtgctgcg gaccgaaacc tacatggaaa tgcactacgg ccatcaggcc 3600

3660

ctccagcaga ggcatcctca gggaccttgt ccctcctcctg tgacacctct gagagtgcag 3720

tctgtgctgc tcctgggagt gcctcagaca gccgctttta gcggccggat tctgcaccag 3780

ccagaaatgg ccgccatgag actgatgccc aagttcctga actccaccaa cagctggtgg 3840

acagtggccc ctagatgtcc tagaagcgcc gtggacatta gccccggacc tacatctcct 3900

agcctggcct cttgtgcctc tgtggccatt ggaaaactgg cactgctggg cgcatctgcc 3960

ccttgtggcg cagctggtgc tagagaggct ggaagatttg ccgtgctggg caccatggtt 4020

atgaagggtg ctgccgcagc ctcctgtcag tctcctccat ctccaagaga gacaacccct 4080

agcagcgacg ccaccagacg gacaagacct attgccgctc agagcaaaaa gaccatgact 4140

gccgccggac tgacccctcg gagaaacctg cagagaaatc tgaaggtcct gggcgccaag 4200

aagatcacct tcctgagaaa cagcagacgc gccacaccta cctctaaggg cagcctgaga 4260

agaaagtacc tgagagtgaa catccgcaag ctcatctgga ccaagcagcg gagcttcctg 4320

tctctgttcc ccaagctgag agccaccgag cagatgacca atgtgggatg cctgctcaag 4380

aaaagcagcc aattgcggcg gagctggcgc aatgtgtccc ggatcagcaa catggaacac 4440

aggctgacca tggacagcgc cctgagcctg acaaagagag agggccctga gacaagcgag 4500

aagaagagga agttgcct 4518

<210> 1170

<211> 4521

<212> DNA

<213> Artificial sequence

566

<220>

<223> polynucleotide 4D

<400> 1170

aacagaaaga ccagaaaggc cgccagagag aagcaccgca agaccgacga caagaagaag 60

aagtccaccg aaaagaccaa gaacgccaag aagatgccct tccagcagcg gcctcctctg 120

cagcctgtta acctggatct gcagcaccag cgcagacgca aaaagaagaa agacaaggtc 180

gaaatccgga atcggagccc taaggccaat agcaccgagt gtcagggcga gcacagcaga 240

gagcagaacc tgctgaatct ggtgcagctg aaccctcaca gccctcacca cagaaaggac 300

gaagaggacc accagaagcg gcaccaccac cacaatcaaa agaaaatgag cgtgcgggct 360

agcgtgtcct tctggacact tttgccaaca accggcgtgc ggacaagagg ctctccactg 420

agactgacac tgtggcctcc acgggttcca ccttctccac ctcttgctct gggccccaga 480

atgcagctgt gtacccagct cgccggttc ttccctatca cacctcctgt gtggcacatc 540

ctgggacctc agagacacac acctcaggct cctctgaggc tgtggtcttg gtgcggcaca 600

agccagacct acttcggcag cagaccatct ccttgcctga gctggtccag cagaattgtg 660

gcccctggac acgccctgag ctgtcggact agaagaagat ggtctagcaa gcggtggatc 720

ttctccaacc agctgaccgt gcagatgctg tgcctgccaa tcacacaccc attcgcctct 780

cagcctctgg gacaagagat gcccaacaac acccagatcc ggctgctgaa gccctgcaga 840

gtgaacgccg tggaagaact gcacagcagg cggatgtctg tgcggatcgt gaagaactgg 900

gtgtcctgct gttacgtgcg gctgtctgct gtgggcctgt ctacttggag tgccctggac 960

gaggaaaaga agaacggccg gatcagcagc ctgaaaaaga aagtgaatgc ctgcagctcc 1020

ctgatcctga gcttccagcg cgtgaaaacc ggcatggaaa tcctgctgtg gattctgctg 1080

1140

tttggaagct ttagcgccga tttcagcgcc cctcagatca ttaagaagat gaagaaacgg 1200

atcatggtgc acgagctgaa gaagaatctg ctgctgccta tgtgctccag cctggctctg 1260

ccaacaggcc tgacatctct gatcctgcct agcagcgatc tggccgtgct gatctctagc 1320

tccaccagcc actttctgat gcggagccct gtgctgccaa gcagcagact gacttgtgcc 1380

tctccacagc tgccccggat gtggacttgg tctagctggc tgaagaacga gggcaacaac 1440

cactggccta gagtggaaat gcctaccggc tggctgctcg tcggctacaa caccagaaac 1500

acagcccaga atcctcggct gcagcagcac ctgaaacact ggcagcagtg caccagagcc 1560

agatggtgtc aagagccctg tcagaccctg accttcttcc atttcttctt ctttttcaaa 1620

acggtgtccc tgtgttgccc cggctggtct aaaggacaac ctcctccacc tggcctgaag 1680

ccttctagcc tggcttctca ggtggccaga accacaggcg tgtaccagca cgtgtggctg 1740

atctgtttcg tgtttttcgt gtgtgtggaa accggcttct gtcacgtggc ccaagtggga 1800

ctcgagagac tggtgtctct gtccggcatc accgacgtga accattgggc ccagcctaag 1860

ccatctccta gcaaaggcac cggcatgaac agacccatcg gcctgagagt tggcaaaggc 1920

gtgtgggcca gactgatcct ggccatgaca ctgaccctga acggcatggc caagaagtcc 1980

cagcagcctc tgcaggtcgt gctgagactg ggaaacctgc ctccattctt cagaggcagc 2040

gaggtgccct ttcctagacc ttgtcggacc aagcctgcct gcacaagcaa ggcctggact 2100

cccagaaaac tcgtggggag agccgtgcgg agaaagggat ctccactctg ggattgctcc 2160

accacaacag gcacacacca cggatgcctg tgggagatcc catctgtgac cctgctggcc 2220

catcctagaa ccgcctctat caccctgcct accacaagcc tgaagggctc cagaaccagc 2280

tgctgccta gatctcaccc cagactgcct aggaccaaat ggcccctgca gtgtctgctg 2340

cacagcctgg gaacaaccgg aacactcgtt tgggaaaggg gcgtcctgca gatgagatcc 2400

ctcgaagccc attgtccact gcagaccttc catctgcacc actgcaggac ccagcctagc 2460

tcttgtcctc ctgcctggat tctgtaccaa ggacagtgga gcggagccgt cgtgggactt 2520

agacctgtgg ctcaccatct gcctagacct atccagggcc tgcactgtcc acaggcacct 2580

catcaacctg ctctgagctg gggaaaatgg cccgtgggaa tgacaggccc tgccagatgt 2640

tgcagatggc ccagacggtg ccagaagaag gttccatcag cctctccatg gggcctcgtg 2700

ctcaaaaaga accggaaaca gatgctgaac cagaagtgtc tggccccttt tagagagagc 2760

accctgggca atttctggat gaagctgctc cagctgcctg tcgtgatcaa cctgaagaga 2820

ctgcagaagt tcaacctgca cctgtgctac taccacagcc agagcaacag aaacaagagc 2880

cgccagatgg aatccctggg catgaagttg cagtcccaga aatcccagca ccactgtgcc 2940

ctgcggaaga tcgagtgggc cacaaagttg tctctcggcg gaaaagccgg acccacaggct 3000

caagagaagg ccacctctcg gattctgaac ctgcaagtgc tcaagaagat cctgcggagc 3060

tttatgaagc tgtaccactc tctggtcatg tgcctgcggc tgaggaccaa gctggaaaag 3120

gctctgagcg ccctgttcat ctggccccag cacagctaca agcccctgtt ctttcacggc 3180

gacctgtgcg agtgggggagg ccaggctcat agacagcggga actggctgaa aaactggaag 3240

cggttcaagc ttaaaaagaa gtacatccag aagaagaagc agaagtctaa caagatcatc 3300

tgcaagggcc accgcatcac catcatctgt atcaagaata tgttcctgca gcgcaagggc 3360

ttcctgaacc tgagccacgt ggaaaagttc tgtagaggcg gccctagcgc cagcgataga 3420

tgctgttctt gtagccccag cggcttcagt gccggcagag gaagatgtcc tgttcaggggc 3480

tgtctgaggc cccacagagt tcaactgctg agaagatggg gccctggctc tcctgctgga 3540

cagaggctgt ctaagggctt tcagctgctc cgttggtggg gaccaggatc tcctgctcct 3600

gagcctagaa agggcccatt tccaccacct gatcctccat ggcctgtgac cgctgtgaca 3660

gtgatggctg gatctgtgcc tagtgctcag tctgtggacg ccctggaatc tcctggacct 3720

ctggctctcg aaggacctag cagccctaga aacctgcttt ggagagagat gagcatcttt 3780

3840

agcctgccaa gctctatgga aatcgccgtg ctgcaagagg acagcaaaaa gatgacccag 3900

aagggccaag ccttcggctc cctgtgtcag cttgtgtccg ctgacttctc tgcccctcaa 3960

gtgacccggc tgcggagcct gaatcatctg ctgatcacct acacacccca catctgcaga 4020

cacaccctgc tgacccctga caccagatgg atctttagca atcagctgca gacagtccag 4080

atgctctgtc tgcctccact gaagcagggc gagagaatca tttggcccct cggcggcttt 4140

cagatcccca acttcttttg gagcctcggc atcggacaca ctggcgcctg gtggcatact 4200

ggaaggcgga gaagagaatg gggcggcagc accgtgtcta gaagccctct gtggatcagc 4260

tccatgatct acctgagagg catggccttc gccagcgaca gcagcatcag aaccaccaga 4320

gtgtcctggt gcagaagatg cggcgccaaa agcgctgccg ccagctctat tgaagctctg 4380

accgctctgt ttacctgccg gaccgccagc atcagcgtga acatcagcac cctgatgtcc 4440

atcctggaag cacttaagaa gaaacccaga gacatctgcc gcatcagctg ccggtgtaga 4500

cgcgagtaca gagtgactat g 4521

<210> 1171

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 1C poly-FSP

<400> 1171

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Ser Thr Arg

20 25 30

Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp Arg

35 40 45

Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys Leu Arg Leu

50 55 60

Arg Asn Thr Ser Pro Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys

65 70 75 80

His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro

85 90 95

Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala

100 105 110

Ala Ser Ser Pro Gly His Pro Leu Gly Pro Gly Asp Cys Pro Arg Thr

115 120 125

Met Ala Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser

130 135 140

Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro

145 150 155 160

Ala Gly Trp Ala Arg Gln Ala Met Thr Leu Lys Ala Leu Val Lys Thr

165 170 175

Trp Thr Gln Phe Arg Asn Tyr Pro Arg Val Gly Ala Gly Thr Gly Gly

180 185 190

Gly Pro Ala Cys Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr

195 200 205

569

Ala Glu Gly Ala Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys

210 215 220

Arg Gln Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys

225 230 235 240

Arg Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro

245 250 255

Lys Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg

260 265 270

Ser Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro

275 280 285

Arg Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser

290 295 300

Arg Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu

305 310 315 320

Pro Ser Cys His Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His

325 330 335

Gln Ala Pro Phe Asp Lys Lys Gly Phe Val Ile Leu Ser Lys Gly Lys

340 345 350

Lys Arg Arg Lys Glu Ile Arg Arg Met Thr Arg Arg Thr Met Thr Thr

355 360 365

Thr Lys Met Met Met Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg

370 375 380

Arg Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr

385 390 395 400

Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln

405 410 415

Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu

420 425 430

Ala Ile Trp Gly Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro

435 440 445

Leu His Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Ile

450 455 460

Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn Thr Leu Ala Ile Arg Phe

465 470 475 480

Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser

485 490 495

Gly Gln Tyr Phe Ser Ser Phe Phe Tyr Thr Ser Asp Arg Thr Lys Cys

570

500 505 510

Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile Thr

515 520 525

Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro His Arg Arg Pro

530 535 540

Gly Trp Lys Gly His His Arg Val Val Phe Ser His His Pro Ser Gly

545 550 555 560

Ser Thr Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg Arg

565 570 575

Glu Lys Lys Gly Cys Arg Glu Met Pro Ser Ser Trp Ser Ser Ser Gly

580 585 590

Arg Thr Ala Asp Lys Lys Asn Val Arg Ser Arg Thr Arg Leu Thr Leu

595 600 605

Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser Ala Val Cys

610 615 620

Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro

625 630 635 640

Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro Pro Glu Val Thr

645 650 655

Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu

660 665 670

Trp Pro Trp Gly Ala Gln Val Arg Asn Lys Val Arg Gln Asn Phe Lys

675 680 685

Arg Asn Leu Ala Lys Lys Thr Leu Thr Asn Ala Leu Arg Ser Met Pro

690 695 700

Lys Ser Arg Asn Gln Val Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln

705 710 715 720

Leu Arg Thr Leu Ala Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser

725 730 735

Gln Ser Leu Ser Arg Lys Gln Ile Pro Ser Gln Ser Met Leu Val Thr

740 745 750

Ala Phe Phe Leu Leu Asp Ile Ile Leu Arg Ser Leu Leu Tyr Ser Arg

755 760 765

Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr Phe

770 775 780

Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe Pro Val

785 790 795 800

571

Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys Thr

805 810 815

Ser His Ser Asn Pro Trp Ser Gln Ser Phe Lys Lys Lys Lys Pro Leu

820 825 830

Thr Phe Ser Pro Ser Val Pro Met Ser Leu Ala Cys Ser Ile Thr Val

835 840 845

Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val Ser Ser Ile

850 855 860

Ser His Trp Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg

865 870 875 880

Gln Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser Asn Trp

885 890 895

Ser Lys Leu Arg Ser Pro Trp Ile Phe Tyr Pro Arg Leu Lys Thr Phe

900 905 910

Gly Val Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr

915 920 925

Met Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser

930 935 940

Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro Trp Arg

945 950 955 960

Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro Thr Ser Cys

965 970 975

Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg Met Pro Gly Ser

980 985 990

Gln Ser Ile Met Met Ser Trp Met Pro Pro Leu Ala Pro Cys Ala Ala

995 1000 1005

Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys Pro Ala His Pro Ala

1010 1015 1020

Arg Ala Pro Thr Ala Val Pro Ala Phe Thr Pro Leu Ser Ala His

1025 1030 1035

Pro Val Pro Val Leu Ser Gly Cys His Leu Ala Val Arg Thr Ser

1040 1045 1050

Met Leu Thr Leu Leu Pro Met Arg His Pro His Arg Ser Pro Pro

1055 1060 1065

Thr Ser Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro Arg Ala

1070 1075 1080

572

Ser Leu Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala Thr

1085 1090 1095

Gly Leu His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe

1100 1105 1110

Leu Leu His Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser His

1115 1120 1125

Arg Leu Arg Leu Arg Leu Pro Arg Thr Gly Glu Lys Asn Asp Leu

1130 1135 1140

Gln Leu Phe Val Met Ser Asp Thr Thr Tyr Lys Ile Tyr Trp Thr

1145 1150 1155

Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His Leu Lys Thr Thr

1160 1165 1170

Ser Leu Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg Met

1175 1180 1185

Trp Trp Leu Lys Leu Ala Pro Leu Gln Val Thr Thr Thr Leu Lys

1190 1195 1200

Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Glu Ala Lys Lys

1205 1210 1215

Ser Met Lys Arg Ser Arg Arg Ser Lys Ile Glu Ala Ser Pro Ile

1220 1225 1230

Gly Ser Ile Met Lys Lys Lys Asp Ser Thr Ile Phe Ile Asn Gln

1235 1240 1245

Gln Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser Val Ser

1250 1255 1260

Lys Lys Arg Leu Pro Lys Leu Asn Gly Ile Trp Glu Val Met Pro

1265 1270 1275

Arg Thr Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr Ser

1280 1285 1290

Gln Asn His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu

1295 1300 1305

Asn Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro Leu

1310 1315 1320

Arg Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys

1325 1330 1335

Ser Ile Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met Asn

1340 1345 1350

573

Ala Lys Lys Met Glu Asp Met Ile Trp Glu Ser Asn Met Gly Gly

1355 1360 1365

Asn Arg Ser Ile Cys Leu Thr Arg Leu Pro Cys Lys Val Thr Glu

1370 1375 1380

Ser Ser Gln Arg Pro Pro Arg Ser Ile Leu Ser Leu Ala Ser Arg

1385 1390 1395

Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly Met Ser

1400 1405 1410

Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys Pro

1415 1420 1425

Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp His

1430 1435 1440

Arg Ser Gly Leu Arg Ala Gln Gln Pro Ser Leu Pro Gly Pro Cys

1445 1450 1455

Ala Ser Leu Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro

1460 1465 1470

Ser Gln Val Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro

1475 1480 1485

Ala Ala Ala Cys Pro Pro Ile Val Phe Phe Leu Ala Pro Tyr Phe

1490 1495 1500

Pro Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg Ala Arg Gln

1505 1510 1515

Ser Cys Cys Leu Pro Gly Leu Thr His Pro Ala His Gln Pro Leu

1520 1525 1530

Gly Ser Met Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1172

<211> 1541

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 2C poly-FSP

<400> 1172

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Arg Gln Ile

20 25 30

Asn Arg Arg Lys Leu Gln Arg Lys Lys Arg Arg Lys Lys Arg Glu Asp

35 40 45

574

His Cys Gly Leu Arg Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser

50 55 60

His Leu Ile Ala His Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe

65 70 75 80

His Pro Arg Leu Leu Thr Arg Lys Lys Pro Gly Lys Asn Pro Arg Arg

85 90 95

Lys Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys

100 105 110

Asn His Leu Tyr Gly Lys Gly Leu Thr His Pro Val Pro Leu Lys Arg

115 120 125

Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val Asn Gln Lys

130 135 140

Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu Ile Val Leu

145 150 155 160

Asn Ser Pro Glu Ser Asn Leu Gln Ala His Pro Gln Val Pro Ala Leu

165 170 175

Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala

180 185 190

Pro Ser Ala Gln Gln Ser Leu Thr Phe Leu Pro Ser Arg Phe Pro Trp

195 200 205

Gly Pro Pro Pro Leu His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu

210 215 220

Leu Val Thr Cys Ile Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys

225 230 235 240

Gln Ser Gln Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu

245 250 255

Met Trp Thr Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln

260 265 270

Glu Arg Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser

275 280 285

Trp Ser Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser

290 295 300

Thr Arg Val Leu Trp Met Pro Thr Ser Thr Val Gly Ala Leu Arg Ala

305 310 315 320

Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg

325 330 335

Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr

575

340 345 350

Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser Ser

355 360 365

Cys Tyr Trp Ser Leu Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu Ala

370 375 380

Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly Arg Met Leu

385 390 395 400

Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly

405 410 415

Val Arg Thr Ser Ser Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu

420 425 430

Arg Met Arg Pro Pro Arg Ser Asn Ser Arg Lys Lys Leu Leu Glu Asp

435 440 445

Met Lys Gln Asn Gly Phe Thr Arg Tyr Lys Lys Arg Gly Val Ile Ala

450 455 460

Trp Arg Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro

465 470 475 480

Gln Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln

485 490 495

Ser Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys Ser Trp Trp

500 505 510

Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys Val Ser

515 520 525

Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly Ile Cys

530 535 540

Trp Lys Trp Pro Val Thr Val Ala Ala Leu Ile Leu Ile Gln His Gly

545 550 555 560

Gly Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile

565 570 575

Cys Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu

580 585 590

Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe His

595 600 605

Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr Thr

610 615 620

Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys

625 630 635 640

576

Tyr Gln Leu Leu Leu Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr

645 650 655

Gln Lys Gly Glu Cys Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu

660 665 670

Phe Ile Leu Lys Gln Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met

675 680 685

Ser Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys

690 695 700

Ala Ser Pro Gln Ile Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr

705 710 715 720

Ile Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn Leu Pro Leu

725 730 735

Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys Ile Thr Met

740 745 750

Val Leu Thr Tyr Pro Asn Cys Val Val His Val Leu Met Phe Val Leu

755 760 765

Phe Lys Ala Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly Ile Tyr Pro

770 775 780

Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Ile Arg Met Cys Ala Leu

785 790 795 800

Ser His Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser Lys

805 810 815

His Cys Met Thr Ala Cys Thr Arg Glu Lys Ile Ile Asn Pro Thr Ile

820 825 830

Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Gly His Arg Glu Gly

835 840 845

Ser Glu Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Trp Leu

850 855 860

Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln Trp Gly

865 870 875 880

Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Asn Ser Val Ile

885 890 895

Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn

900 905 910

Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala

915 920 925

577

Val Leu Lys Leu Leu Asn Met Asp Leu Ala Ser Pro Gln Ile Met Gln

930 935 940

Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn Leu

945 950 955 960

Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly Ala Ala Pro Pro

965 970 975

His Arg Leu Leu Gly Pro Ser Arg Trp Ile Leu Phe His Val Arg Asn

980 985 990

Ile Val Tyr Gly Ile Gln Val Met Leu Cys Arg Arg Arg Asn Ser Arg

995 1000 1005

Lys Gln Ser Gln Val Gln Leu Ala Ile Gln Val Leu Leu Val Asp

1010 1015 1020

Lys His Lys Tyr Phe Leu Phe Gly Pro Tyr Pro Ile Ser Glu Gly

1025 1030 1035

Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu Met

1040 1045 1050

Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His Ala Ile

1055 1060 1065

Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser Ser

1070 1075 1080

Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys Lys

1085 1090 1095

Phe Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Gln Lys Lys

1100 1105 1110

Gly His Met Ile Phe Ser Pro Lys Phe Phe Leu Thr Phe Ser Gln

1115 1120 1125

Arg Thr Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln

1130 1135 1140

Cys Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile

1145 1150 1155

His His Arg Lys Asn Pro Leu Leu Pro Met Arg Met Ser Arg Thr

1160 1165 1170

Pro Pro Arg Arg Val Cys Gly Ser Pro Pro Leu His Leu Cys Gln

1175 1180 1185

Pro Leu Arg Gly Gly Lys Lys Arg Ser Lys Phe Ala Phe Trp Lys

1190 1195 1200

578

Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr Gly Cys Trp

1205 1210 1215

Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr

1220 1225 1230

Pro Arg Gly Asp Trp Lys Arg His Pro Arg Arg Glu Leu Ser

1235 1240 1245

Leu Glu Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Arg

1250 1255 1260

Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln

1265 1270 1275

Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu

1280 1285 1290

Ala Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr

1295 1300 1305

Leu Phe Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu

1310 1315 1320

Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg

1325 1330 1335

Lys Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg

1340 1345 1350

Leu Phe Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His Leu

1355 1360 1365

Gln Asn Phe Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr Ile

1370 1375 1380

Asn Ile Leu Thr Arg Ala Phe Ile His Thr Pro Arg Ser Pro Gly

1385 1390 1395

Cys Ser Val Tyr Ala Asn Met Cys Trp Arg Lys Met Gln Ser Leu

1400 1405 1410

Asn His Pro Ser Arg Leu Thr Asn Cys His Pro Ala Trp Leu Ser

1415 1420 1425

Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe His Val

1430 1435 1440

Leu Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met Pro Gln

1445 1450 1455

Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp Asp Lys

1460 1465 1470

Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser Cys Leu Glu Lys

579

1475 1480 1485

Lys Ala Thr Trp Met Arg Arg Thr Pro Gly Thr Ser Leu Gln Cys

1490 1495 1500

Tyr Leu Asn Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly Ile

1505 1510 1515

Pro Ser Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Gly Tyr Pro

1520 1525 1530

Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540

<210> 1173

<211> 1546

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 3C poly-FSP

<400> 1173

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Pro Ile Pro

20 25 30

Pro His Leu Arg His Pro Leu Leu Leu Lys Asn Lys Lys Arg Arg Lys

35 40 45

Leu Arg Pro Lys Ser Arg Lys Lys Gly His Thr Lys Asn Arg Arg Lys

50 55 60

Ala Lys Arg Lys Pro Gln Ile Arg Asn Ser Arg Arg Ala Thr Pro Thr

65 70 75 80

Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Gly Ala Ala Ala

85 90 95

Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr Thr Pro Ser Ser

100 105 110

Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys Thr

115 120 125

Met Thr Ala Ala Gly Leu Thr Pro Ser Gln Lys Lys Asp Pro Ala Gly

130 135 140

Lys Leu Lys Lys Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe

145 150 155 160

Ser Arg Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Cys Thr Asn

165 170 175

580

Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val Phe

180 185 190

Ile Leu Leu Leu Ser Asp Lys Lys Ser Ile Pro His Val Pro Lys Ala

195 200 205

Thr Ala Ala His Ser Val Thr Val Lys Leu Gln Ser Arg Arg Asn Arg

210 215 220

Arg Tyr Arg Lys Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Ser

225 230 235 240

Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu Ser Val

245 250 255

Ser Val Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn

260 265 270

Trp Asn Leu Val Val Trp Ile Leu Asn Leu Lys Met Met Leu Lys Lys

275 280 285

Lys Tyr Leu Val Gln Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu Gly

290 295 300

Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg

305 310 315 320

Lys Ala Met Thr Val Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe

325 330 335

Met Arg Cys Val Gln Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro

340 345 350

Leu Gly Arg Met Asp Leu Asp Gly Met Leu Thr Val Ile Gly Gln Gly

355 360 365

Leu Val Gly Met Thr Pro Arg Asp Leu Gly Thr Val Ala Gly Ala Thr

370 375 380

Ala Gln Ile Gln Arg Pro Ile Gln Val Gln Thr Gly Gly Arg Leu Arg

385 390 395 400

Gly Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln

405 410 415

Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg Thr Glu Leu

420 425 430

Pro Leu Thr Phe Ser Thr Leu Leu Trp Asp Ser Arg His Asp Leu Pro

435 440 445

Arg Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln Met Pro Phe

450 455 460

Trp Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro

581

465 470 475 480

Ser Thr Trp Gly Trp Arg Thr Phe Ala Arg Gln Pro Ser Arg Pro Thr

485 490 495

Gly Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met

500 505 510

Ser Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met

515 520 525

Pro Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser

530 535 540

Pro Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile

545 550 555 560

Ser Arg Ala Thr Trp Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu

565 570 575

Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln Gln Asn Thr Arg

580 585 590

Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln Arg

595 600 605

Arg Gly Lys Leu Ser Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu

610 615 620

Leu Leu Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser

625 630 635 640

Arg Ile Ser Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe

645 650 655

Leu Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val

660 665 670

Gly Cys Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly Asp Arg Val

675 680 685

Phe Leu Val Lys Ile Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn

690 695 700

Thr Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly

705 710 715 720

Ala His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met

725 730 735

Asn Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro

740 745 750

Leu Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly Lys Arg

755 760 765

582

Cys Asn Pro Cys Cys Arg Leu Thr Trp Met Ser His Leu Asn Arg Lys

770 775 780

Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu Thr Met Met Met Ser

785 790 795 800

Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro

805 810 815

Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro Cys

820 825 830

Leu Leu Leu Leu His His Arg His His Leu Ala Phe Leu Glu Met Ala

835 840 845

Cys Ser Ser Gln His Thr His Ser Ile Lys Ile Trp Ile Ser Phe Ser

850 855 860

His Glu Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg Leu Thr

865 870 875 880

Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg Gly

885 890 895

Gly Thr Thr Ile Gly Glu Val Thr Ser Ser Leu Phe Phe Ser His Cys

900 905 910

Leu Cys Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser Pro

915 920 925

Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala Ile Gly

930 935 940

Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala

945 950 955 960

Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr Met Val Met Lys Asn

965 970 975

Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser Leu Thr Lys Arg

980 985 990

Glu Gly Pro Glu Thr Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn

995 1000 1005

Val Ser Ser Val Leu Lys Pro Phe Leu Phe Thr Val Pro Ile Glu

1010 1015 1020

Asp Met Arg Glu Cys Thr Leu Gly Arg Asn Arg Met Asn Val Ser

1025 1030 1035

Ser Val Leu Lys Pro Cys Leu Ile Pro Val Pro Ile Cys Lys Lys

1040 1045 1050

583

Lys Leu Leu Leu Lys Leu Leu Arg Gly Ala Ser Ser Ser Thr Ser

1055 1060 1065

Arg Asp Ser Arg Ser Phe Phe Thr Ser Ala His Pro Ser Arg Pro

1070 1075 1080

Cys Arg Met Arg Leu Tyr Gln Met Leu Gln Lys Gln Cys Cys Tyr

1085 1090 1095

Asn Leu Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg

1100 1105 1110

Arg Gln Gly Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly Phe

1115 1120 1125

Leu Asn Gln Gln Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys Leu

1130 1135 1140

Leu Met Phe Ile Lys Arg Trp Lys Pro Ala Ala Thr Lys Lys Gly

1145 1150 1155

Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln

1160 1165 1170

Gly His Leu Leu Met Ile Leu Leu His Asn Arg Gly Pro Pro Leu

1175 1180 1185

His Thr Arg Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser Arg Lys

1190 1195 1200

Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro Ala Phe Ser Gly

1205 1210 1215

Arg Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met Pro

1220 1225 1230

Lys Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr Val His Lys Lys

1235 1240 1245

Arg Gly Leu Phe His Gln Met Lys Cys Leu Thr Ala Val Asp Cys

1250 1255 1260

Leu Gln Ala Phe Trp Asp Met Ser Glu Lys Lys Arg Lys Leu Pro

1265 1270 1275

Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala Asn Arg Asn

1280 1285 1290

Ile Glu Ile Thr Pro Gln Arg Ser Lys Ala His Leu Lys Lys Thr

1295 1300 1305

Ser Ala Lys Gly Ala Lys Lys Ile Thr Phe Leu Cys Leu Arg Val

1310 1315 1320

584

Arg Cys Gly Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Trp Leu

1325 1330 1335

Lys Lys Ser Thr Lys Ser Arg Leu Cys Phe Phe Tyr Leu Leu Tyr

1340 1345 1350

Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Thr Pro Pro Pro Leu

1355 1360 1365

Ile Leu Trp Pro Gln Ala Ala Pro Gly Glu Lys Leu Lys His Cys

1370 1375 1380

Phe Lys Ala Trp Gly Val Asp Pro Pro Gly Ser Val Trp Ile Leu

1385 1390 1395

Pro Cys Phe Leu Ile His Leu Arg Lys Val Asp Thr His His Leu

1400 1405 1410

Gln Val Val Thr Lys Thr Val Thr Ala Thr Thr Ala Asp Gly Gln

1415 1420 1425

Arg Met Arg Arg Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp Ala

1430 1435 1440

Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser His Phe Pro

1445 1450 1455

Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln Leu Arg

1460 1465 1470

Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val Ala

1475 1480 1485

Met Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu Ser

1490 1495 1500

Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro Val

1505 1510 1515

Thr Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro Gln

1520 1525 1530

Thr Ala Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1174

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 4C poly-FSP

<400> 1174

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

585

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Asp Ser Lys

20 25 30

Lys Met Thr Gln Lys Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys

35 40 45

His Arg Lys Thr Asp Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys

50 55 60

Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn

65 70 75 80

Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn

85 90 95

Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys Asp Glu Glu

100 105 110

Asp His Gln Lys Arg His His His His Asn Gln Lys Lys Met Ser Val

115 120 125

Asn Glu Gly Asn Asn His Trp Pro Arg Val Glu Met Pro Thr Gly Trp

130 135 140

Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu

145 150 155 160

Gln Gln His Leu Lys His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys

165 170 175

Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe

180 185 190

Lys Thr Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro

195 200 205

Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr

210 215 220

Thr Gly Val Tyr Gln His Val Trp Leu Ile Cys Phe Val Phe Phe Val

225 230 235 240

Cys Val Glu Thr Gly Phe Cys His Val Ala Gln Val Gly Leu Glu Arg

245 250 255

Leu Val Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro

260 265 270

Lys Pro Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu

275 280 285

Arg Val Gly Lys Gly Val Trp Ala Arg Leu Trp Leu Lys Asn Trp Lys

290 295 300

586

Arg Phe Lys Leu Lys Lys Lys Tyr Thr Gly Met Glu Ile Leu Leu Trp

305 310 315 320

Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu

325 330 335

Met Gln Leu Cys Leu Lys Lys Lys Val Asn Ala Cys Ser Asn Ala Lys

340 345 350

Lys Met Pro Phe Gln Cys Arg Cys Arg Arg Glu Tyr Arg Val Thr Met

355 360 365

Ile Gln Lys Lys Lys Gln Lys Ser Pro Leu Phe Phe His Gly Asp Leu

370 375 380

Cys Arg Pro Ser Pro Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro

385 390 395 400

Gly His Ala Leu Ser Cys Arg Thr Arg Arg Arg Trp Ser Ser Ser Lys Met

405 410 415

Ala Lys Lys Ser Gln Gln Pro Leu Cys Arg Gly Gly Pro Ser Ala Ser

420 425 430

Asp Arg Cys Cys Ser Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly

435 440 445

Arg Cys Pro Val Gln Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu

450 455 460

Arg Arg Trp Gly Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly

465 470 475 480

Phe Gln Leu Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro

485 490 495

Arg Lys Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala

500 505 510

Val Thr Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala

515 520 525

Leu Glu Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg

530 535 540

Asn Leu Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Asn

545 550 555 560

Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg

565 570 575

Gln Met Glu Ser Leu Gly Met Lys Leu Gln Ile Thr His Pro Phe Ala

580 585 590

Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile Arg Leu

587

595 600 605

Leu Lys Pro Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala

610 615 620

Pro Gln Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln

625 630 635 640

Thr Tyr Phe Gly Ser Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg

645 650 655

Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu

660 665 670

Ala Ile Leu Ala Met Thr Leu Thr Leu Asn Gly Ser Pro Leu Trp Asp

675 680 685

Cys Ser Thr Thr Thr Gly Thr His His Gly Cys Leu Trp Glu Ile Pro

690 695 700

Ser Val Thr Leu Leu Ala Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys

705 710 715 720

Cys Gln Arg Pro Pro Leu Gln Pro Val Glu Glu Lys Lys Asn Gly Arg

725 730 735

Ile Ser Ser Val Pro Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg Met

740 745 750

Gln Leu Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro Val

755 760 765

Trp His Ile Leu Gly Pro Gln Arg His Thr Pro Leu Leu Thr Pro Asp

770 775 780

Thr Arg Trp Ile Phe Ser Asn Gln Leu Gln Thr Val Gln Met Leu Cys

785 790 795 800

Leu Pro Pro Leu Lys Gln Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly

805 810 815

Phe Gln Ile Pro Asn Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly

820 825 830

Ala Trp Trp His Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr

835 840 845

Val Ser Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly

850 855 860

Met Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp

865 870 875 880

Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Asp Lys Lys

885 890 895

588

Lys Lys Ser Thr Glu Lys Thr Lys Asn Lys Ile Ile Cys Lys Gly His

900 905 910

Arg Ile Thr Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe Phe

915 920 925

Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys Cys Gln

930 935 940

Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu His Pro Arg Thr Ala

945 950 955 960

Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly Ser Arg Thr Ser Cys

965 970 975

Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr Lys Trp Pro Leu Gln

980 985 990

Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr Leu Val Trp Glu Arg

995 1000 1005

Gly Val Leu Gln Met Arg Ser Leu Glu Ala His Cys Pro Leu Gln

1010 1015 1020

Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser Ser Cys Pro

1025 1030 1035

Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala Val Val

1040 1045 1050

Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile Gln Gly

1055 1060 1065

Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser Trp Gly

1070 1075 1080

Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys Arg Trp

1085 1090 1095

Pro Arg Arg Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu Thr

1100 1105 1110

Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu Ile Ser Ser

1115 1120 1125

Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro Ser Ser

1130 1135 1140

Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp Thr Trp

1145 1150 1155

Ser Ser Trp Leu Lys Glu Trp Gly Gly Gln Ala His Arg Gln Arg

1160 1165 1170

589

Asn Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg Arg Met

1175 1180 1185

Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr Val Arg

1190 1195 1200

Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp Ser Leu

1205 1210 1215

Ile Leu Ser Phe Gln Arg Val Lys Thr Ser Arg Ile Leu Asn Leu

1220 1225 1230

Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys Leu Tyr His

1235 1240 1245

Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu Glu Lys Ala

1250 1255 1260

Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr Lys Gly Gln

1265 1270 1275

Ala Phe Gly Ser Leu Cys Gln Leu Val Ser Ala Asp Phe Ser Ala

1280 1285 1290

Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His Leu Leu Ile Arg

1295 1300 1305

Trp Ile Phe Ser Asn Gln Leu Thr Val Gln Met Leu Cys Leu Pro

1310 1315 1320

Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val His Val Leu Lys

1325 1330 1335

Lys Asn Arg Lys Gln Ile Ile Cys Ile Lys Asn Met Phe Leu Gln

1340 1345 1350

Arg Lys Gly Phe Leu Asn Leu Ser His Val Glu Lys Phe Arg Glu

1355 1360 1365

Ser Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln Leu Pro Pro

1370 1375 1380

Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg

1385 1390 1395

Ala Val Arg Arg Lys Gly Val Val Ile Asn Leu Lys Arg Leu Gln

1400 1405 1410

Lys Phe Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr

1415 1420 1425

Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro

1430 1435 1440

590

Pro Arg Ser Gln Lys Ser Gln His His Cys Ala Leu Arg Lys Ile

1445 1450 1455

Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro Gln

1460 1465 1470

Ala Gln Glu Lys Ala Asn Ile Leu Asn Ser Leu Pro Ser Ser Met

1475 1480 1485

Glu Ile Ala Val Leu Gln Glu Met Leu Asn Gln Lys Cys Leu Ala

1490 1495 1500

Pro Phe Glu Leu Lys Lys Asn Leu Leu Leu Pro Thr Tyr Thr Pro

1505 1510 1515

His Ile Cys Arg His Thr Leu Lys Lys Lys Pro Arg Asp Ile Cys

1520 1525 1530

Arg Ile Ser Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1175

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 1C poly-FSP

<400> 1175

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

591

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Ser Thr Arg Arg Glu Met Pro Ser

225 230 235 240

Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp Arg Ala Ala Leu Gly Lys

245 250 255

Glu Val Lys Arg Ser Gln Ser Lys Leu Arg Leu Arg Asn Thr Ser Pro

260 265 270

Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu Ser

275 280 285

Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln Gln Pro

290 295 300

Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala Ala Ser Ser Pro Gly

305 310 315 320

His Pro Leu Gly Pro Gly Asp Cys Pro Arg Thr Met Ala Gly Pro Ser

325 330 335

Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser Arg Lys Thr Trp Gln

340 345 350

Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro Ala Gly Trp Ala Arg

355 360 365

Gln Ala Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe Arg

370 375 380

Asn Tyr Pro Arg Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys Val

385 390 395 400

Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala Ala

405 410 415

Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro Ala

420 425 430

592

Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg Arg

435 440 445

Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu Arg

450 455 460

Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro Gln

465 470 475 480

Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro Ser

485 490 495

Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu Ala

500 505 510

Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro Ser Cys His Pro

515 520 525

Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln Ala Pro Phe Asp

530 535 540

Lys Lys Gly Phe Val Ile Leu Ser Lys Gly Lys Lys Arg Arg Lys Glu

545 550 555 560

Ile Arg Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met Met Met

565 570 575

Met Arg Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met Thr Met

580 585 590

Met Thr Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro Arg

595 600 605

Thr Ser Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln Arg

610 615 620

Arg Pro Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp Gly Arg

625 630 635 640

Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu His Arg Trp Leu

645 650 655

Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Ile Ala Lys Lys Arg Ile

660 665 670

Lys Leu Arg Arg Asn Thr Leu Ala Ile Arg Phe Ile Ser Ala Pro Gln

675 680 685

Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser Gly Gln Tyr Phe Ser

690 695 700

Ser Phe Phe Tyr Thr Ser Asp Arg Thr Lys Cys Glu Ile Cys Val Lys

705 710 715 720

Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile Thr Pro Gln Asp Pro His

593

725 730 735

Leu Asp Pro Val Cys His Pro His Arg Arg Pro Gly Trp Lys Gly His

740 745 750

His Arg Val Val Phe Ser His Pro Ser Gly Ser Thr Lys Ser His

755 760 765

Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg Arg Glu Lys Lys Gly Cys

770 775 780

Arg Glu Met Pro Ser Ser Trp Ser Ser Ser Gly Arg Thr Ala Asp Lys

785 790 795 800

Lys Asn Val Arg Ser Arg Thr Arg Leu Thr Leu Thr Gly Thr Arg Ser

805 810 815

Ser Gly Thr Arg Ala Ser Pro Ser Ala Val Cys Leu Cys Cys Trp Thr

820 825 830

Cys Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro Ser Gly Leu Arg Ala

835 840 845

Pro Val Gly Ala Gln Ala Pro Pro Glu Val Thr Leu Arg Ala Ser Cys

850 855 860

Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly Leu Trp Pro Trp Gly Ala

865 870 875 880

Gln Val Arg Asn Lys Val Arg Gln Asn Phe Lys Arg Asn Leu Ala Lys

885 890 895

Lys Thr Leu Thr Asn Ala Leu Arg Ser Met Pro Lys Ser Arg Asn Gln

900 905 910

Val Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu Ala

915 920 925

Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser Arg

930 935 940

Lys Gln Ile Pro Ser Gln Ser Met Leu Val Thr Ala Phe Phe Leu Leu

945 950 955 960

Asp Ile Ile Leu Arg Ser Leu Leu Tyr Ser Arg Met Cys Leu Val Gln

965 970 975

Ile Pro Thr His Thr Trp Glu Pro Asp Tyr Phe Val Ser Leu Pro Met

980 985 990

Met Phe Ser Trp Ala Thr Gln Ser Phe Pro Val Gly Ala Val Phe Leu

995 1000 1005

Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys Thr Ser His Ser Asn

1010 1015 1020

594

Pro Trp Ser Gln Ser Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser

1025 1030 1035

Pro Ser Val Pro Met Ser Leu Ala Cys Ser Ile Thr Val Ser Phe

1040 1045 1050

Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val Ser Ser Ile Ser

1055 1060 1065

His Trp Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys Ser Arg

1070 1075 1080

Gln Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser Asn

1085 1090 1095

Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe Tyr Pro Arg Leu Lys

1100 1105 1110

Thr Phe Gly Val Pro Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser

1115 1120 1125

Leu Ser Thr Met Met Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly

1130 1135 1140

Arg Arg Ala Ser Val Pro Cys Arg Pro Met Ile Gly Ser Ala Arg

1145 1150 1155

Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser Ala Met Gly Val

1160 1165 1170

Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr

1175 1180 1185

Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met Met Ser Trp Met

1190 1195 1200

Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro

1205 1210 1215

Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro Thr Ala Val Pro

1220 1225 1230

Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro Val Leu Ser Gly

1235 1240 1245

Cys His Leu Ala Val Arg Thr Ser Met Leu Thr Leu Leu Pro Met

1250 1255 1260

Arg His Pro His Arg Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu

1265 1270 1275

Glu Ala Phe Pro Leu Pro Arg Ala Ser Leu Arg Ala Thr Val Pro

1280 1285 1290

595

Arg His Ser Ser Val Leu Ala Thr Gly Leu His Leu His Arg Gln

1295 1300 1305

Ile Ser Leu Pro Leu Arg Gly Phe Leu Leu His Gln Pro Leu Pro

1310 1315 1320

Gly Gln His Leu Trp Leu Ser His Arg Leu Arg Leu Arg Leu Pro

1325 1330 1335

Arg Thr Gly Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser Asp

1340 1345 1350

Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys

1355 1360 1365

Gly Asn Leu His Leu Lys Thr Thr Ser Leu Arg Arg Ala Arg Pro

1370 1375 1380

Thr Ser Cys Ser Thr Cys Arg Met Trp Trp Leu Lys Leu Ala Pro

1385 1390 1395

Leu Gln Val Thr Thr Thr Leu Lys Leu Ile Leu Val Met Leu Glu

1400 1405 1410

Ile Val Thr Glu Glu Ala Lys Lys Ser Met Lys Arg Ser Arg Arg

1415 1420 1425

Ser Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile Met Lys Lys Lys

1430 1435 1440

Asp Ser Thr Ile Phe Ile Asn Gln Gln Leu Lys Trp Thr Pro His

1445 1450 1455

Ile Leu Lys Ser Ala Ser Val Ser Lys Lys Arg Leu Pro Lys Leu

1460 1465 1470

Asn Gly Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys Ile

1475 1480 1485

Ser Val Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile

1490 1495 1500

Thr Glu Cys Val Arg Thr Ala Leu Asn Gln Arg Lys Val Val Lys

1505 1510 1515

Asn Pro Ser Gln Pro Val Pro Leu Arg Asn Asp Gly Ile Thr Asn

1520 1525 1530

Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser Leu Lys Lys Ser

1535 1540 1545

Leu Glu Lys Met Pro Leu Met Asn Ala Lys Lys Met Glu Asp Met

1550 1555 1560

596

Ile Trp Glu Ser Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr

1565 1570 1575

Arg Leu Pro Cys Lys Val Thr Glu Ser Ser Gln Arg Pro Pro Arg

1580 1585 1590

Ser Ile Leu Ser Leu Ala Ser Arg Ala Gly Gln Phe Pro Val Trp

1595 1600 1605

Arg Arg Thr Ser Arg Gly Met Ser Leu Gln Trp Lys Ser Gly Asn

1610 1615 1620

Ala Cys Thr Pro Leu Arg Cys Pro Ser Arg Ser Pro Ala Thr Phe

1625 1630 1635

Leu Thr Ala Leu Asn Gly Trp His Arg Ser Gly Leu Arg Ala Gln

1640 1645 1650

Gln Pro Ser Leu Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu

1655 1660 1665

Ser Gln Pro Pro Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala

1670 1675 1680

Thr Leu Arg Cys Pro Ala Val Pro Ala Ala Ala Cys Pro Pro Ile

1685 1690 1695

Val Phe Phe Leu Ala Pro Tyr Phe Pro Thr Pro Leu Ser Ile Ser

1700 1705 1710

Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu

1715 1720 1725

Thr His Pro Ala His Gln Pro Leu Gly Ser Met Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1176

<211> 1744

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 2C poly-FSP

<400> 1176

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

597

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Arg Gln Ile Asn Arg Arg Lys Leu

225 230 235 240

Gln Arg Lys Lys Arg Arg Lys Lys Arg Glu Asp His Cys Gly Leu Arg

245 250 255

Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu Ile Ala His

260 265 270

Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro Arg Leu Leu

275 280 285

Thr Arg Lys Lys Pro Gly Lys Asn Pro Arg Arg Lys Thr Trp Lys Met

290 295 300

Arg Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn His Leu Tyr Gly

305 310 315 320

Lys Gly Leu Thr His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn Lys

325 330 335

598

Arg Asn Lys Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile Lys

340 345 350

Arg Lys Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro Glu Ser

355 360 365

Asn Leu Gln Ala His Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp

370 375 380

Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln Gln

385 390 395 400

Ser Leu Thr Phe Leu Pro Ser Arg Phe Pro Trp Gly Pro Pro Pro Leu

405 410 415

His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr Cys Ile

420 425 430

Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp

435 440 445

Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr Gln Trp

450 455 460

Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys Ser

465 470 475 480

Ser Trp Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser Leu Arg Asp

485 490 495

Pro Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg Val Leu Trp

500 505 510

Met Pro Thr Ser Thr Val Gly Ala Leu Arg Ala Thr Ala Pro Ala Thr

515 520 525

Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg Ala Ala Thr Thr Ser

530 535 540

Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr Ser Ser Ser Ser Ser Trp

545 550 555 560

Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser Cys Tyr Trp Ser Leu

565 570 575

Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr Arg Thr

580 585 590

Arg Ser Arg Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg Ser Ser

595 600 605

Leu Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr Ser Ser

610 615 620

Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro

599

625 630 635 640

Arg Ser Asn Ser Arg Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly

645 650 655

Phe Thr Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg Arg Ala Thr

660 665 670

Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala Arg Leu Cys

675 680 685

Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser Arg Cys Ser Leu

690 695 700

Met Glu Gln Ile Pro His Leu Lys Ser Trp Trp Cys Thr Arg Gly Gly

705 710 715 720

Gly Lys Arg Lys Lys Lys Met Cys Lys Val Ser Gly Gly Cys Thr Ala

725 730 735

Cys Val Glu Arg Cys Pro Trp Lys Gly Ile Cys Trp Lys Trp Pro Val

740 745 750

Thr Val Ala Ala Leu Ile Leu Ile Gln His Gly Gly Ile Phe Thr Phe

755 760 765

Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu Ser

770 775 780

Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr Ile Ile

785 790 795 800

Glu Thr Asn Val Phe Val Leu Asn Leu Phe His Val Pro Val Asn Gln

805 810 815

Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr Thr Glu Lys Ala Trp Val

820 825 830

Met Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys Tyr Gln Leu Leu Leu

835 840 845

Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys

850 855 860

Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln

865 870 875 880

Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Ser Arg Ser His Leu

885 890 895

Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Ala Ser Pro Gln Ile

900 905 910

Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Arg Arg Lys Trp

915 920 925

600

Gln Ile Arg His Val Leu Lys Asn Leu Pro Leu Glu Asn Thr Gln Ile

930 935 940

Pro Arg Thr Ser Pro Leu Trp Lys Ile Thr Met Val Leu Thr Tyr Pro

945 950 955 960

Asn Cys Val Val His Val Leu Met Phe Val Leu Phe Lys Ala Asp Gln

965 970 975

Ser Glu Ser Ser Leu Ser Ser Gly Ile Tyr Pro Pro Ser Thr Cys Ser

980 985 990

Leu Pro Cys Arg Gly Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe

995 1000 1005

Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser Lys His Cys Met Thr

1010 1015 1020

Ala Cys Thr Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser Cys Pro

1025 1030 1035

Phe Gln Ser Pro Thr Lys Arg Leu Gly His Arg Glu Gly Ser Glu

1040 1045 1050

Ser Asp Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Trp Leu Pro

1055 1060 1065

Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln Trp Gly

1070 1075 1080

Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Asn Ser Val

1085 1090 1095

Ile Val His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser

1100 1105 1110

Tyr Asn Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile

1115 1120 1125

Glu Phe Ala Val Leu Lys Leu Leu Asn Met Asp Leu Ala Ser Pro

1130 1135 1140

Gln Ile Met Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg

1145 1150 1155

Arg Met Gln Asn Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu

1160 1165 1170

Pro Gly Ala Ala Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp

1175 1180 1185

Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln Val Met

1190 1195 1200

601

Leu Cys Arg Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln Leu

1205 1210 1215

Ala Ile Gln Val Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe

1220 1225 1230

Gly Pro Tyr Pro Ile Ser Glu Gly Leu Thr Met Thr Thr Gly Arg

1235 1240 1245

Asn Ile Asn Ser Asn Ser Leu Met Thr Asn Cys Leu Phe Ser Pro

1250 1255 1260

Ile Phe Leu Cys His His Ala Ile Met His Arg Lys Met Thr Ser

1265 1270 1275

Pro His Phe Arg Leu Phe Ser Ser Lys Ile Pro His Pro Gln Val

1280 1285 1290

Pro Ser Val Val Ala Leu Cys Lys Phe Leu Ala Asn Gly Leu Trp

1295 1300 1305

Phe Asn Leu Ile Phe Gln Lys Lys Gly His Met Ile Phe Ser Pro

1310 1315 1320

Lys Phe Phe Leu Thr Phe Ser Gln Arg Thr Leu Glu Ser Ala Gly

1325 1330 1335

Leu Phe Pro Trp Leu His Thr Gln Cys Arg Asn Lys Lys Thr Lys

1340 1345 1350

Leu Trp Phe Ser Leu Ile Asn Ile His His Arg Lys Asn Pro Leu

1355 1360 1365

Leu Pro Met Arg Met Ser Arg Thr Pro Pro Arg Arg Val Cys Gly

1370 1375 1380

Ser Pro Pro Leu His Leu Cys Gln Pro Leu Arg Gly Gly Lys Lys

1385 1390 1395

Arg Ser Lys Phe Ala Phe Trp Lys Pro Ser Gln Gly Ile Ser

1400 1405 1410

Ser Cys Leu Met Thr Gly Cys Trp Gly Ser Cys Gly Arg Ala Cys

1415 1420 1425

Ser Cys Gly Arg Gly Cys Gly Thr Pro Arg Gly Asp Trp Lys Arg

1430 1435 1440

His Pro Pro Arg Arg Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr

1445 1450 1455

Leu His Gln Gln Lys Thr Gln Arg Lys Arg Met Lys Thr Gly Ala

1460 1465 1470

602

Ile Gln Glu Lys Gly Ala Ile Gln Glu Lys Gly Ala Met Thr Lys

1475 1480 1485

Arg Ile Cys Val Val Asn Phe Glu Ala Cys Arg Glu Met Glu Ser

1490 1495 1500

Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe Glu Ala His Met Ala

1505 1510 1515

Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg Ala Ser Leu Ile

1520 1525 1530

Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg Asn Gln Val

1535 1540 1545

Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly Tyr Gln

1550 1555 1560

Asn Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe Ser

1565 1570 1575

Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr Arg Ala Phe

1580 1585 1590

Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr Ala Asn Met

1595 1600 1605

Cys Trp Arg Lys Met Gln Ser Leu Asn His Pro Ser Arg Leu Thr

1610 1615 1620

Asn Cys His Pro Ala Trp Leu Ser Pro Ile Ser Ala Cys Pro Arg

1625 1630 1635

Leu Leu Pro Tyr Pro Phe His Val Leu Ala Leu Glu Val Thr Phe

1640 1645 1650

Pro Pro Gly Thr Ala Met Pro Gln Met Gln Leu Gln Ala Leu Ala

1655 1660 1665

Met Trp Glu Lys Gly Trp Asp Lys Gln Glu Lys Lys Ser Arg Lys

1670 1675 1680

Leu Met Ser Ser Cys Leu Glu Lys Lys Ala Thr Trp Met Arg Arg

1685 1690 1695

Thr Pro Gly Thr Ser Leu Gln Cys Tyr Leu Asn Thr His Pro Cys

1700 1705 1710

Thr Met Leu Leu Pro Gln Gly Ile Pro Ser Leu Asn Ala Gln Trp

1715 1720 1725

Met Phe Leu Arg Ile Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala

1730 1735 1740

Ser

603

<210> 1177

<211> 1749

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 3C poly-FSP

<400> 1177

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Pro Ile Pro Pro His Leu Arg His

225 230 235 240

604

Pro Leu Leu Leu Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys Ser

245 250 255

Arg Lys Lys Gly His Thr Lys Asn Arg Arg Lys Ala Lys Arg Lys Pro

260 265 270

Gln Ile Arg Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu

275 280 285

Arg Arg Lys Tyr Leu Arg Val Gly Ala Ala Ala Ala Ser Cys Gln Ser

290 295 300

Pro Pro Ser Pro Arg Glu Thr Thr Pro Ser Ser Asp Ala Thr Arg Arg

305 310 315 320

Thr Arg Pro Ile Ala Ala Gln Ser Lys Lys Thr Met Thr Ala Ala Gly

325 330 335

Leu Thr Pro Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys Gln

340 345 350

Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser Arg Arg Gly Met

355 360 365

Lys Ser Ser Val Arg Leu Lys Asn Cys Thr Asn Leu Ser Val Pro Met

370 375 380

Met Leu Thr Ile Leu Ile Trp Lys Arg Val Phe Ile Leu Leu Leu Ser

385 390 395 400

Asp Lys Lys Ser Ile Pro His Val Pro Lys Ala Thr Ala Ala His Ser

405 410 415

Val Thr Val Lys Leu Gln Ser Arg Arg Asn Arg Arg Tyr Arg Lys Ile

420 425 430

Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Ser Asn Gly Met Glu Ala

435 440 445

Cys Gly Thr His Pro Thr Pro Arg Leu Ser Val Ser Val Ile Thr Met

450 455 460

Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn Leu Val Val

465 470 475 480

Trp Ile Leu Asn Leu Lys Met Met Leu Lys Lys Lys Tyr Leu Val Gln

485 490 495

Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr

500 505 510

Lys Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg Lys Ala Met Thr Val

515 520 525

Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe Met Arg Cys Val Gln

605

530 535 540

Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro Leu Gly Arg Met Asp

545 550 555 560

Leu Asp Gly Met Leu Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr

565 570 575

Pro Arg Asp Leu Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg

580 585 590

Pro Ile Gln Val Gln Thr Gly Gly Arg Leu Arg Gly Pro Arg Arg Lys

595 600 605

Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln Ser Leu Gln Lys Ser

610 615 620

Gln Val Phe Gln Trp Val Leu Arg Thr Glu Leu Pro Leu Thr Phe Ser

625 630 635 640

Thr Leu Leu Trp Asp Ser Arg His Asp Leu Pro Arg Met Leu Pro Ile

645 650 655

Ser Ser Pro Leu His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr Phe

660 665 670

Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp Gly Trp

675 680 685

Arg Thr Phe Ala Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro Thr

690 695 700

Thr Arg Leu Met Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu Leu

705 710 715 720

Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu His

725 730 735

Pro Gln Trp Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys Arg

740 745 750

Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr Trp

755 760 765

Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu Arg Leu Arg Ile Pro

770 775 780

Ser Leu Arg Lys Tyr Arg Gln Gln Asn Thr Arg Ser Thr Asn Leu Glu

785 790 795 800

Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu Ser

805 810 815

Leu Thr Thr Leu Ser Phe Val Leu Ile Glu Glu Leu Leu Lys Lys Ser

820 825 830

606

Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser Arg Ile Ser Asn Ile

835 840 845

Arg Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe Pro

850 855 860

Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys Arg Leu Asp

865 870 875 880

Phe His Pro Arg Ser Ser Ser Gly Asp Arg Val Phe Leu Val Lys Ile

885 890 895

Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn

900 905 910

Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His His Cys Gln

915 920 925

Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp Lys Lys Met

930 935 940

Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp Ser Ser Pro

945 950 955 960

Leu Gln Leu Leu Leu His Pro His Gly Lys Arg Cys Asn Pro Cys Cys

965 970 975

Arg Leu Thr Trp Met Ser His Leu Asn Arg Lys Leu His Leu Thr Arg

980 985 990

Ser Cys Leu Ile Asp Leu Thr Met Met Met Ser Gln Lys Leu Trp Lys

995 1000 1005

Asn Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro Arg Gln His Leu

1010 1015 1020

Leu Pro Gln Tyr Pro Leu His Pro Pro Pro Pro Cys Leu Leu Leu

1025 1030 1035

Leu His His Arg His His Leu Ala Phe Leu Glu Met Ala Cys Ser

1040 1045 1050

Ser Gln His Thr His Ser Ile Lys Ile Trp Ile Ser Phe Ser His

1055 1060 1065

Glu Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg Leu Thr

1070 1075 1080

Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala Leu Arg Ala Arg

1085 1090 1095

Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Ser Leu Phe Phe Ser

1100 1105 1110

607

His Cys Leu Cys Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp

1115 1120 1125

Ile Ser Pro Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser

1130 1135 1140

Val Ala Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys

1145 1150 1155

Gly Ala Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val Leu Gly

1160 1165 1170

Thr Met Val Met Lys Asn Met Glu His Arg Leu Thr Met Asp Ser

1175 1180 1185

Ala Leu Ser Leu Thr Lys Arg Glu Gly Pro Glu Thr Gly Pro Val

1190 1195 1200

Tyr Tyr Val Cys Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro

1205 1210 1215

Phe Leu Phe Thr Val Pro Ile Glu Asp Met Arg Glu Cys Thr Leu

1220 1225 1230

Gly Arg Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro Cys Leu

1235 1240 1245

Ile Pro Val Pro Ile Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu

1250 1255 1260

Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe Phe

1265 1270 1275

Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln

1280 1285 1290

Met Leu Gln Lys Gln Cys Cys Tyr Asn Leu Val Ile Lys His Pro

1295 1300 1305

Leu Ile Phe Arg Ile Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp

1310 1315 1320

Gly Val Val Gln Ser Leu Gly Phe Leu Asn Gln Gln Ile Ile Leu

1325 1330 1335

Gln Lys Glu Phe Phe Ile Cys Leu Leu Met Phe Ile Lys Arg Trp

1340 1345 1350

Lys Pro Ala Ala Thr Lys Lys Gly Lys Asn Ser Pro Cys Cys Gln

1355 1360 1365

Lys Lys Leu Arg Val Gln Asn Gln Gly His Leu Leu Met Ile Leu

1370 1375 1380

608

Leu His Asn Arg Gly Pro Pro Leu His Thr Arg Phe Glu Lys Lys

1385 1390 1395

Arg Pro Glu Trp Leu Ser Arg Lys Ser Gly Val Met Lys Cys Trp

1400 1405 1410

Asp Leu Cys Pro Ala Phe Ser Gly Arg Ile Leu His Gln Pro Glu

1415 1420 1425

Met Ala Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr Asn

1430 1435 1440

Ser Trp Trp Thr Val His Lys Lys Arg Gly Leu Phe His Gln Met

1445 1450 1455

Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe Trp Asp Met

1460 1465 1470

Ser Glu Lys Lys Arg Lys Leu Pro Met Ile Arg Lys Ile Gln Lys

1475 1480 1485

Arg Arg Arg Thr Ala Asn Arg Asn Ile Glu Ile Thr Pro Gln Arg

1490 1495 1500

Ser Lys Ala His Leu Lys Lys Thr Ser Ala Lys Gly Ala Lys Lys

1505 1510 1515

Ile Thr Phe Leu Cys Leu Arg Val Arg Cys Gly Gly Trp Leu Cys

1520 1525 1530

Gly Trp Thr Pro Arg Gly Trp Leu Lys Lys Ser Thr Lys Ser Arg

1535 1540 1545

Leu Cys Phe Phe Tyr Leu Leu Tyr Tyr Leu Phe Phe Leu Thr Val

1550 1555 1560

Cys Gly Phe Thr Pro Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala

1565 1570 1575

Pro Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly Val Asp

1580 1585 1590

Pro Pro Gly Ser Val Trp Ile Leu Pro Cys Phe Leu Ile His Leu

1595 1600 1605

Arg Lys Val Asp Thr His His Leu Gln Val Val Thr Lys Thr Val

1610 1615 1620

Thr Ala Thr Thr Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys

1625 1630 1635

Lys Ala Cys Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln

1640 1645 1650

Ala Pro Pro Lys Ser His Phe Pro Pro His Pro Ser Gly Pro Ser

609

1655 1660 1665

Arg Thr Ser Ser Leu Gln Leu Arg Thr Tyr Met Glu Met His Tyr

1670 1675 1680

Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu Leu Cys

1685 1690 1695

Arg Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His Pro

1700 1705 1710

Gln Gly Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser

1715 1720 1725

Val Leu Leu Leu Gly Val Pro Gln Thr Ala Gly Tyr Pro Tyr Asp

1730 1735 1740

Val Pro Asp Tyr Ala Ser

1745

<210> 1178

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 4C poly-FSP

<400> 1178

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

610

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Asp Ser Lys Lys Met Thr Gln Lys

225 230 235 240

Asn Arg Lys Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp

245 250 255

Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys Lys Lys Lys Asp Lys

260 265 270

Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn Ser Thr Glu Cys Gln

275 280 285

Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn Leu Val Gln Leu Asn

290 295 300

Pro His Ser Pro His His Arg Lys Asp Glu Glu Asp His Gln Lys Arg

305 310 315 320

His His His His Asn Gln Lys Lys Met Ser Val Asn Glu Gly Asn Asn

325 330 335

His Trp Pro Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr

340 345 350

Asn Thr Arg Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys

355 360 365

His Trp Gln Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln

370 375 380

Thr Leu Thr Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu

385 390 395 400

Cys Cys Pro Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys

405 410 415

Pro Ser Ser Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln

420 425 430

His Val Trp Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly

611

435 440 445

Phe Cys His Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser

450 455 460

Gly Ile Thr Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser

465 470 475 480

Lys Gly Thr Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly

485 490 495

Val Trp Ala Arg Leu Trp Leu Lys Asn Trp Lys Arg Phe Lys Leu Lys

500 505 510

Lys Lys Tyr Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu Lys Met

515 520 525

Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu Cys Leu

530 535 540

Lys Lys Lys Val Asn Ala Cys Ser Asn Ala Lys Lys Met Pro Phe Gln

545 550 555 560

Cys Arg Cys Arg Arg Glu Tyr Arg Val Thr Met Ile Gln Lys Lys Lys

565 570 575

Gln Lys Ser Pro Leu Phe Phe His Gly Asp Leu Cys Arg Pro Ser Pro

580 585 590

Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro Gly His Ala Leu Ser

595 600 605

Cys Arg Thr Arg Arg Arg Trp Ser Ser Lys Met Ala Lys Lys Ser Gln

610 615 620

Gln Pro Leu Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser

625 630 635 640

Cys Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln

645 650 655

Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly Pro

660 665 670

Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg

675 680 685

Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe

690 695 700

Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val Thr Val Met Ala

705 710 715 720

Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly

725 730 735

612

Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg

740 745 750

Glu Met Ser Ile Phe Leu Pro Gly Ile Phe Asn Leu His Leu Cys Tyr

755 760 765

Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg Gln Met Glu Ser Leu

770 775 780

Gly Met Lys Leu Gln Ile Thr His Pro Phe Ala Ser Gln Pro Leu Gly

785 790 795 800

Gln Glu Met Pro Asn Asn Thr Gln Ile Arg Leu Leu Lys Pro Gly Gln

805 810 815

Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala Pro Gln Gln Ala Pro

820 825 830

Leu Arg Leu Trp Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser

835 840 845

Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile Ser

850 855 860

Val Asn Ile Ser Thr Leu Met Ser Ile Leu Glu Ala Ile Leu Ala Met

865 870 875 880

Thr Leu Thr Leu Asn Gly Ser Pro Leu Trp Asp Cys Ser Thr Thr Thr

885 890 895

Gly Thr His His Gly Cys Leu Trp Glu Ile Pro Ser Val Thr Leu Leu

900 905 910

Ala Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys Cys Gln Arg Pro Pro

915 920 925

Leu Gln Pro Val Glu Glu Lys Lys Asn Gly Arg Ile Ser Ser Val Pro

930 935 940

Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys Thr Gln

945 950 955 960

Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile Leu Gly

965 970 975

Pro Gln Arg His Thr Pro Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe

980 985 990

Ser Asn Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys

995 1000 1005

Gln Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro

1010 1015 1020

613

Asn Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp

1025 1030 1035

His Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser

1040 1045 1050

Arg Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met

1055 1060 1065

Ala Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp

1070 1075 1080

Cys Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Asp Lys

1085 1090 1095

Lys Lys Lys Ser Thr Glu Lys Thr Lys Asn Lys Ile Ile Cys Lys

1100 1105 1110

Gly His Arg Ile Thr Gln Val Val Leu Arg Leu Gly Asn Leu Pro

1115 1120 1125

Pro Phe Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg

1130 1135 1140

Thr Lys Cys Gln Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu

1145 1150 1155

His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys

1160 1165 1170

Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu Pro

1175 1180 1185

Arg Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr

1190 1195 1200

Thr Gly Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg Ser

1205 1210 1215

Leu Glu Ala His Cys Pro Leu Gln Thr Phe His Leu His His Cys

1220 1225 1230

Arg Thr Gln Pro Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln

1235 1240 1245

Gly Gln Trp Ser Gly Ala Val Val Gly Leu Arg Pro Val Ala His

1250 1255 1260

His Leu Pro Arg Pro Ile Gln Gly Leu His Cys Pro Gln Ala Pro

1265 1270 1275

His Gln Pro Ala Leu Ser Trp Gly Lys Trp Pro Val Gly Met Thr

1280 1285 1290

614

Gly Pro Ala Arg Cys Cys Arg Trp Pro Arg Arg Met Cys Ser Ser

1295 1300 1305

Leu Ala Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu Pro Ser Ser

1310 1315 1320

Asp Leu Ala Val Leu Ile Ser Ser Ser Thr Ser His Phe Leu Met

1325 1330 1335

Arg Ser Pro Val Leu Pro Ser Ser Arg Leu Thr Cys Ala Ser Pro

1340 1345 1350

Gln Leu Pro Arg Met Trp Thr Trp Ser Ser Trp Leu Lys Glu Trp

1355 1360 1365

Gly Gly Gln Ala His Arg Gln Arg Asn Cys Arg Val Asn Ala Val

1370 1375 1380

Glu Glu Leu His Ser Arg Arg Met Ser Val Arg Ile Val Lys Asn

1385 1390 1395

Trp Val Ser Cys Cys Tyr Val Arg Leu Ser Ala Val Gly Leu Ser

1400 1405 1410

Thr Trp Ser Ala Leu Asp Ser Leu Ile Leu Ser Phe Gln Arg Val

1415 1420 1425

Lys Thr Ser Arg Ile Leu Asn Leu Gln Val Leu Lys Lys Ile Leu

1430 1435 1440

Arg Ser Phe Met Lys Leu Tyr His Ser Leu Val Met Cys Leu Arg

1445 1450 1455

Leu Arg Thr Lys Leu Glu Lys Ala Leu Ser Ala Leu Phe Ile Trp

1460 1465 1470

Pro Gln His Ser Tyr Lys Gly Gln Ala Phe Gly Ser Leu Cys Gln

1475 1480 1485

Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr Arg Leu Arg

1490 1495 1500

Ser Leu Asn His Leu Leu Ile Arg Trp Ile Phe Ser Asn Gln Leu

1505 1510 1515

Thr Val Gln Met Leu Cys Leu Pro Ile Ile Lys Lys Met Lys Lys

1520 1525 1530

Arg Ile Met Val His Val Leu Lys Lys Asn Arg Lys Gln Ile Ile

1535 1540 1545

Cys Ile Lys Asn Met Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu

1550 1555 1560

Ser His Val Glu Lys Phe Arg Glu Ser Thr Leu Gly Asn Phe Trp

615

1565 1570 1575

Met Lys Leu Leu Gln Leu Pro Pro Ala Cys Thr Ser Lys Ala Trp

1580 1585 1590

Thr Pro Arg Lys Leu Val Gly Arg Ala Val Arg Arg Lys Gly Val

1595 1600 1605

Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Arg Ala Ser Val Ser

1610 1615 1620

Phe Trp Thr Leu Leu Pro Thr Thr Gly Val Arg Thr Arg Gly Ser

1625 1630 1635

Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg Ser Gln Lys Ser Gln

1640 1645 1650

His His Cys Ala Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser

1655 1660 1665

Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln Glu Lys Ala Asn Ile

1670 1675 1680

Leu Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu

1685 1690 1695

Met Leu Asn Gln Lys Cys Leu Ala Pro Phe Glu Leu Lys Lys Asn

1700 1705 1710

Leu Leu Leu Pro Thr Tyr Thr Pro His Ile Cys Arg His Thr Leu

1715 1720 1725

Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile Ser Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1179

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 1D poly-FSP

<400> 1179

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Ala Asp Lys

20 25 30

Lys Asn Val Arg Ser Ala Ser Ser Pro Gly His Pro Leu Gly Pro Arg

35 40 45

616

Arg Met Thr Arg Arg Thr Met Thr Thr Thr Lys Met Met Met Met Arg

50 55 60

Arg Thr Lys Arg Arg Arg Arg Lys Arg Arg Arg Met Thr Met Met Thr

65 70 75 80

Arg Arg Thr Leu Leu Thr Lys Lys Thr Cys Leu Thr Pro Arg Thr Ser

85 90 95

Ala Ala Thr Lys Ala Leu Pro Arg Gln Thr Ile Ser Gln Arg Arg Pro

100 105 110

Ile Gln Thr Pro Pro Gly Thr Ser Leu Ala Ile Trp Gly Arg Arg Ser

115 120 125

Lys Ile Glu Ala Ser Pro Ile Gly Ser Ile Thr Pro Leu Ser Ile Ser

130 135 140

Arg Pro Thr Ser Arg Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu Thr

145 150 155 160

His Pro Ala His Gln Pro Leu Gly Ser Met Val Gly Ala Gly Thr Gly

165 170 175

Gly Gly Pro Ala Cys Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp

180 185 190

Thr Ala Glu Gly Ala Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro

195 200 205

Cys Arg Gln Leu Pro Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val

210 215 220

Lys Arg Arg Arg Arg Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val

225 230 235 240

Pro Lys Lys Arg Glu Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala

245 250 255

Arg Ser Ala Leu Pro Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu

260 265 270

Pro Arg Ala Leu Pro Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu

275 280 285

Ser Arg Phe Trp Leu Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His

290 295 300

Leu Pro Ser Cys His Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro

305 310 315 320

His Gln Ala Pro Gly Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser

325 330 335

Asp Thr Thr Tyr Lys Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys

617

340 345 350

Gly Asn Leu His Leu Lys Thr Thr Ser Leu Asn Ala Lys Lys Met Glu

355 360 365

Asp Met Ile Trp Glu Ser Asn Gln Gln Leu Lys Trp Thr Pro His Ile

370 375 380

Leu Lys Ser Ala Ser Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn

385 390 395 400

Ser Thr Arg Arg Glu Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly

405 410 415

Pro Trp Arg Ala Ala Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys

420 425 430

Leu Arg Leu Arg Asn Thr Ser Pro Phe Asp Lys Lys Gly Phe Val Ile

435 440 445

Leu Ser Asn Met Gly Gly Asn Arg Ser Ile Cys Leu Thr Ile Val Phe

450 455 460

Phe Leu Ala Pro Tyr Phe Pro Met Pro Ser Ser Trp Ser Ser Ser Gly

465 470 475 480

Arg Thr Arg Leu Pro Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu His

485 490 495

Arg Trp Leu Gln Gln Thr Thr Gly Cys Ser Gln Arg Arg Gly Asp Cys

500 505 510

Pro Arg Thr Met Ala Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly

515 520 525

Arg Ala Ser Arg Lys Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala

530 535 540

Gly Gly Pro Ala Gly Trp Ala Arg Gln Ala Arg Lys Arg Lys Arg Glu

545 550 555 560

Lys Arg Ser Gln Lys Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys

565 570 575

Arg Arg Lys Ile Ser Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe

580 585 590

Glu Ala Lys Lys Ser Met Lys Arg Ser Gln Arg Pro Pro Arg Ser Ile

595 600 605

Leu Ser Leu Phe Lys Lys Lys Lys Pro Leu Thr Phe Ser Pro Ser Val

610 615 620

Ser Lys Lys Arg Leu Pro Lys Leu Lys Gly Lys Lys Arg Arg Lys Glu

625 630 635 640

618

Ile Gln Arg Lys Val Val Lys Asn Pro Ser Gln Pro Val Pro Leu Arg

645 650 655

Asn Asp Gly Ile Thr Asn Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile

660 665 670

Ser Leu Lys Lys Ser Leu Glu Lys Met Pro Leu Met Arg Ser Leu Leu

675 680 685

Tyr Ser Arg Met Cys Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro

690 695 700

Asp Tyr Phe Val Ser Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser

705 710 715 720

Phe Pro Val Gly Ala Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys

725 730 735

Ser Cys Thr Ser His Ser Asn Pro Trp Ser Gln Ser Thr Leu Ala Ile

740 745 750

Arg Phe Ile Ser Ala Pro Gln Pro Ser Ser Ser Thr Val Thr Ala Ala

755 760 765

Pro Ser Gly Gln Tyr Phe Ala Leu Arg Ser Met Pro Lys Ser Arg Asn

770 775 780

Gln Val Leu Phe Arg Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu

785 790 795 800

Ala Pro Pro Tyr Met Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser

805 810 815

Arg Lys Gln Ile Pro Ser Gln Ser Met Leu Val Arg Thr Arg Leu Thr

820 825 830

Leu Thr Gly Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser Ala Val

835 840 845

Cys Leu Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala Ala Ser

850 855 860

Pro Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro Pro Glu Val

865 870 875 880

Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala Cys Ala Gly

885 890 895

Leu Trp Pro Trp Gly Ala Gln Val Arg Met Lys Lys Lys Asp Ser Thr

900 905 910

Ile Phe Ile Tyr Pro Arg Leu Lys Thr Phe Gly Val Pro Leu Gly Ser

915 920 925

619

Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr Met Met Ile Ile Ser Thr

930 935 940

Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser Val Pro Cys Arg Pro Met

945 950 955 960

Ile Gly Ser Ala Arg Pro Gly Pro Trp Arg Thr Ser Ala Met Pro Ser

965 970 975

Ala Met Gly Val Ala Leu Pro Thr Ser Cys Glu Ser Gly Arg Ser Pro

980 985 990

Pro Ala Thr Gly Gly Arg Met Pro Gly Ser Gln Ser Ile Met Met Ser

995 1000 1005

Trp Met Pro Pro Pro Leu Ala Pro Cys Ala Ala Cys Pro Cys Ser Pro

1010 1015 1020

Ala Pro Thr Leu Cys Pro Ala His Pro Ala Arg Ala Pro Thr Ala

1025 1030 1035

Val Pro Ala Phe Thr Pro Leu Ser Ala His Pro Val Pro Val Leu

1040 1045 1050

Ser Gly Cys His Leu Ala Val Arg Thr Ser Met Leu Thr Leu Leu

1055 1060 1065

Pro Met Met Thr Leu Lys Ala Leu Val Lys Thr Trp Thr Gln Phe

1070 1075 1080

Arg Asn Tyr Pro Arg Arg His Pro His Arg Ser Pro Pro Thr Ser

1085 1090 1095

Cys Pro Pro Leu Leu Glu Ala Phe Pro Leu Pro Arg Ala Ser Leu

1100 1105 1110

Arg Ala Thr Val Pro Arg His Ser Ser Val Leu Ala Thr Gly Leu

1115 1120 1125

His Leu His Arg Gln Ile Ser Leu Pro Leu Arg Gly Phe Leu Leu

1130 1135 1140

His Gln Pro Leu Pro Gly Gln His Leu Trp Leu Ser His Arg Leu

1145 1150 1155

Arg Leu Arg Leu Pro Arg Thr Thr Glu Ile Gln Leu Thr Met Asn

1160 1165 1170

Asp Ser Lys His Lys Leu Glu Ser Pro Ala Leu Lys Gln Val Ser

1175 1180 1185

Pro Ala Ser Pro Pro Thr Gln Gln Pro Gln Thr Pro Gln Asp Ser

1190 1195 1200

620

Arg Gln Val Leu Ala Val Pro Met Ser Leu Ala Cys Ser Ile Thr

1205 1210 1215

Val Ser Phe Val Gly Gln Ile Ser Thr Leu Val Leu Asp Val Ser

1220 1225 1230

Ser Ile Ser His Trp Asn Gly Ile Trp Glu Val Met Pro Arg Thr

1235 1240 1245

Leu Glu Gly Cys Ile Ser Val Lys Asp His Arg Thr Ser Gln Asn

1250 1255 1260

His Ser Lys Cys Ile Thr Glu Cys Val Arg Thr Ala Leu Asn Thr

1265 1270 1275

Lys Cys Glu Ile Cys Val Lys Asn Thr Ala Ile Leu Ala Ile Thr

1280 1285 1290

Leu Ile Thr Pro Gln Asp Pro His Leu Asp Pro Val Cys His Pro

1295 1300 1305

His Arg Arg Pro Gly Trp Lys Gly His His Arg Val Val Phe Ser

1310 1315 1320

His His Pro Ser Gly Ser Thr Lys Ser His Trp Gln Gln Arg Glu

1325 1330 1335

Arg Gly Gly Gly Arg Arg Glu Lys Lys Gly Cys Arg Glu Ala Ser

1340 1345 1350

Arg Ala Gly Gln Phe Pro Val Trp Arg Arg Thr Ser Arg Gly Met

1355 1360 1365

Ser Leu Gln Trp Lys Ser Gly Asn Ala Cys Thr Pro Leu Arg Cys

1370 1375 1380

Pro Ser Arg Ser Pro Ala Thr Phe Leu Thr Ala Leu Asn Gly Trp

1385 1390 1395

His Arg Ser Gly Leu Arg Ala Gln Thr Ala Phe Phe Leu Leu Asp

1400 1405 1410

Ile Ile Leu Arg Arg Ala Arg Pro Thr Ser Cys Ser Thr Cys Arg

1415 1420 1425

Met Trp Trp Arg Leu Pro Cys Lys Val Thr Glu Ser Ser Leu Ala

1430 1435 1440

Lys Lys Thr Leu Thr Asn Gln Pro Ser Leu Pro Gly Pro Cys Ala

1445 1450 1455

Ser Leu Leu Ser Thr Leu Ser Gln Pro Pro Pro Gln Ala Pro Ser

1460 1465 1470

Gln Val Trp Thr Ala Ala Thr Leu Arg Cys Pro Ala Val Pro Ala

1475 1480 1485

Ala Ala Cys Pro Pro Leu Lys Leu Ala Pro Leu Gln Val Thr Thr

1490 1495 1500

Thr Leu Lys Leu Ile Leu Val Met Leu Glu Ile Val Thr Glu Asn

1505 1510 1515

Lys Val Arg Gln Asn Phe Lys Arg Asn Ser Ser Phe Phe Tyr Thr

1520 1525 1530

Ser Asp Arg Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1180

<211> 1541

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 2D poly-FSP

<400> 1180

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Arg Lys Met

20 25 30

Gln Ser Leu Asn His Pro Ser Arg Leu Thr Asn Asn Pro Arg Arg Lys

35 40 45

Thr Trp Lys Met Arg Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn

50 55 60

His Leu Tyr Arg Gln Ile Asn Arg Arg Lys Leu Gln Arg Lys Lys Ser

65 70 75 80

Arg Ser His Leu Asp Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Arg

85 90 95

Ala Arg Asn Arg Asn Met Lys Lys Thr Met Ser His Leu Ile Ala His

100 105 110

Gln Ala Leu Lys Met Ser Gly Leu Arg Leu Phe His Pro Arg Leu Leu

115 120 125

Thr Arg Lys Lys Pro Gly Lys Gln Lys Lys Gly His Met Ile Phe Ser

130 135 140

Arg Arg Lys Trp Gln Ile Arg His Val Leu Lys Asn Leu Ile Pro Ser

145 150 155 160

Leu Asn Ala Gln Trp Met Phe Leu Arg Ile Ala Ser Pro Gln Ile Met

165 170 175

622

Gln Arg Thr Ser Ala Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn

180 185 190

Leu Asp Asn Leu Gly Val Pro Trp Pro Leu Leu Pro Gly Leu Leu Leu

195 200 205

Ala His Met Tyr Gly Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys

210 215 220

Val Cys Leu Gln Cys Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln

225 230 235 240

Pro Leu Asp Leu Lys Ile Ser Lys Thr Phe Met Ala Ser Pro Gln Ile

245 250 255

Met Gln Arg Met Ser Ala Val Asn Phe Glu Thr Ile Leu Gln Ala His

260 265 270

Pro Gln Val Pro Ala Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg

275 280 285

Gly Pro Leu Thr Ser Ala Pro Ser Ala Gln Gln Ser Leu Thr Arg Thr

290 295 300

Leu Glu Ser Ala Gly Leu Phe Pro Trp Leu His Thr Gln Cys Arg Ala

305 310 315 320

Phe Ile His Thr Pro Arg Ser Pro Gly Cys Ser Val Tyr Ala Asn Met

325 330 335

Cys Trp Arg Tyr Lys Lys Arg Gly Val Ile Ala Trp Arg Ile Tyr Pro

340 345 350

Pro Ser Thr Cys Ser Leu Pro Cys Arg Gly Leu Ile Leu Ile Gln His

355 360 365

Gly Gly Ile Phe Thr Phe Arg Phe Trp Val Glu Lys Leu Ser Trp Asn

370 375 380

Ile Cys Lys Asn Leu Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu

385 390 395 400

Leu Tyr Val Tyr Ile Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe

405 410 415

His Val Pro Val Asn Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr

420 425 430

Thr Glu Lys Ala Trp Val Met Glu Leu Glu Trp Leu Ile Gln Gln Gln

435 440 445

Cys Tyr Gln Arg Lys Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly

450 455 460

Ala Ile Gln Glu Lys Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn

465 470 475 480

Phe Glu Ala Cys Arg Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile

485 490 495

Thr Leu Phe Glu Ala His Met Ala Arg Cys Thr Ser Ile Asn Val Leu

500 505 510

Cys Val Arg Ala Ser Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys

515 520 525

Met Lys Arg Asn Gln Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe

530 535 540

Ile Lys Gly Tyr Gln Asn Asn His Phe Thr Phe His Leu Gln Asn Phe

545 550 555 560

Ala Phe Phe Ser Ser Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr

565 570 575

Phe Leu Pro Ser Arg Phe Pro Trp Gly Pro Pro Pro Leu Gly Ala Leu

580 585 590

Arg Ala Thr Ala Pro Ala Thr Ala Ala Pro Gln Ala Gln Thr Pro His

595 600 605

Trp Arg Ala Ala Thr Thr Ser Ser Met Ser Ser Ser Pro Arg Ala Glu

610 615 620

Pro Thr Ser Ser Ser Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys Ser

625 630 635 640

Ser Ser Cys Tyr Trp Ser Leu Ser Ser Ser Trp Pro Pro Ala Gly Ala Ala

645 650 655

Glu Ala Thr Asn Thr Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly Arg

660 665 670

Met Leu Thr Trp Arg Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys Phe

675 680 685

Thr Gly Val Arg Thr Ser Ser Arg Cys Ser Leu Met Glu Gln Ile Pro

690 695 700

His Leu Lys Ser Asn Ser Arg Lys Lys Leu Leu Glu Asp Met Lys Gln

705 710 715 720

Asn Gly Phe Thr Arg Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln

725 730 735

Leu Ala Ile Gln Val Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe

740 745 750

Gly Arg Ala Thr Phe Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln

755 760 765

624

Ala Arg Leu Cys Leu Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser

770 775 780

Arg His Pro Ser Trp Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro

785 790 795 800

Arg Ser His Trp Asn Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr

805 810 815

Cys Ile Leu Tyr His Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln

820 825 830

Arg Trp Leu Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr

835 840 845

Trp Trp Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys

850 855 860

Val Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly

865 870 875 880

Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Met Ser Arg Thr Pro

885 890 895

Pro Arg Arg Val Cys Gly His Arg Glu Gly Ser Glu Ser Asp Asn Leu

900 905 910

Gly Leu Leu Tyr Ser Phe Pro Pro Val Leu Phe Lys Ala Asp Gln Ser

915 920 925

Glu Ser Ser Leu Ser Ser Gly Asn Ser Val Ile Val His Lys Ile Val

930 935 940

Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn Cys Glu Arg Arg Ile

945 950 955 960

Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe Ala Val Leu Lys Leu Leu

965 970 975

Asn Met Asp Leu Gly Lys Gly Leu Thr His Pro Val Pro Leu Lys Arg

980 985 990

Thr Leu Gln Asn Lys Arg Asn Lys Asn Ile Lys Arg Val Asn Gln Lys

995 1000 1005

Lys Gly Asn Ile Lys Arg Lys Ser Ile Leu Leu His Leu Ile Val

1010 1015 1020

Leu Asn Ser Pro Glu Ser Asn Arg Glu Lys Ile Ile Asn Pro Thr

1025 1030 1035

Ile Ser Cys Pro Phe Gln Ser Pro Thr Lys Arg Leu Gly Ser Pro

1040 1045 1050

625

Pro Leu His Leu Cys Gln Pro Leu Arg Pro Tyr Pro Ile Ser Glu

1055 1060 1065

Gly Leu Thr Met Thr Thr Gly Arg Asn Ile Asn Ser Asn Ser Leu

1070 1075 1080

Met Thr Asn Cys Leu Phe Ser Pro Ile Phe Leu Cys His His Ala

1085 1090 1095

Ile Met His Arg Lys Met Thr Ser Pro His Phe Arg Leu Phe Ser

1100 1105 1110

Ser Lys Ile Pro His Pro Gln Val Pro Ser Val Val Ala Leu Cys

1115 1120 1125

Lys Phe Thr His Pro Cys Thr Met Leu Leu Pro Gln Gly Ala Phe

1130 1135 1140

Trp Lys Pro Pro Ser Gln Gly Ile Ser Ser Cys Leu Met Thr Gly

1145 1150 1155

Cys Trp Gln Trp Ser Ser Val Thr Ser Leu Glu Trp Thr Ala Gln

1160 1165 1170

Glu Arg Gly Cys Ser Ser Trp Leu Met Lys Gln Thr Trp Met Lys

1175 1180 1185

Ser Trp Ser Leu Arg Asp Pro Ser Tyr Arg Ser Ile Leu Glu Tyr

1190 1195 1200

Val Ser Thr Arg Val Leu Trp Met Pro Thr Ser Thr Val Pro Lys

1205 1210 1215

Phe Phe Leu Thr Phe Ser Gln Ile Arg Met Cys Ala Leu Ser His

1220 1225 1230

Pro Ser Phe Tyr Ile Ser Ser Thr Val Cys Phe Leu Ser Lys His

1235 1240 1245

Cys Met Thr Ala Cys Thr Pro Gly Thr Ser Leu Gln Cys Tyr Leu

1250 1255 1260

Asn Leu Ala Asn Gly Leu Trp Phe Asn Leu Ile Phe Arg Asn Lys

1265 1270 1275

Lys Thr Lys Leu Trp Phe Ser Leu Ile Asn Ile His His Arg Lys

1280 1285 1290

Asn Pro Leu Leu Pro Met Arg Leu Glu Lys Lys Ala Thr Trp Met

1295 1300 1305

Arg Arg Thr Glu Leu Ser Leu Glu Pro Trp Ile Pro Tyr Leu His

1310 1315 1320

626

Gln Gln Lys Thr Gln Trp Leu Pro Met Arg Ser Leu Gly Glu Pro

1325 1330 1335

Trp Arg Ala Gly Phe Gln Trp Gly Glu Ser Pro Trp Gln Val Met

1340 1345 1350

Ala Ser Leu Thr Gly Arg Arg Lys Lys Arg Glu Asp His Cys Gly

1355 1360 1365

Leu Gln Glu Lys Lys Ser Arg Lys Leu Met Ser Ser Cys Ala Ala

1370 1375 1380

Pro Pro His Arg Leu Leu Gly Pro Ser Arg Trp Gly Ser Cys Gly

1385 1390 1395

Arg Ala Cys Ser Cys Gly Arg Gly Cys Gly Thr Pro Arg Gly Asp

1400 1405 1410

Trp Lys Arg His Pro Pro Arg Arg Ile Leu Phe His Val Arg Asn

1415 1420 1425

Ile Val Tyr Gly Ile Gln Val Met Leu Cys Cys His Pro Ala Trp

1430 1435 1440

Leu Ser Pro Ile Ser Ala Cys Pro Arg Leu Leu Pro Tyr Pro Phe

1445 1450 1455

His Val Leu Ala Leu Glu Val Thr Phe Pro Pro Gly Thr Ala Met

1460 1465 1470

Pro Gln Met Gln Leu Gln Ala Leu Ala Met Trp Glu Lys Gly Trp

1475 1480 1485

Asp Lys Pro Leu Glu Asn Thr Gln Ile Pro Arg Thr Ser Pro Leu

1490 1495 1500

Trp Lys Ile Thr Met Val Leu Thr Tyr Pro Asn Cys Val Val His

1505 1510 1515

Val Leu Met Phe Gly Gly Lys Lys Arg Ser Lys Phe Gly Tyr Pro

1520 1525 1530

Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540

<210> 1181

<211> 1546

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 3D poly-FSP

<400> 1181

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

627

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Gln Asn Thr

20 25 30

Arg Ser Thr Asn Leu Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln

35 40 45

Arg Arg Gly Lys Leu Ser Ser Arg Lys Lys Gly His Thr Lys Asn Arg

50 55 60

Arg Lys Ala Lys Arg Lys Pro Gln Ile Ser Gln Lys Lys Asp Pro Ala

65 70 75 80

Gly Lys Leu Lys Lys His Leu Asn Arg Lys Leu His Leu Thr Arg Ser

85 90 95

Cys Leu Ile Asp Leu Thr Met Met Met Ser Gln Lys Leu Trp Lys Asn

100 105 110

Gln Arg Lys Arg Ile Pro Leu Pro Ser Pro Arg Gln His Leu Leu Pro

115 120 125

Gln Tyr Pro Leu His Pro Pro Pro Pro Cys Leu Leu Leu Leu His His

130 135 140

Arg His His Leu Ala Phe Leu Glu Met Ala Cys Ser Ser Gln His Thr

145 150 155 160

His Ser Ile Lys Ile Trp Ile Ser Phe Ser His Glu Asp Leu Pro Arg

165 170 175

Met Leu Pro Ile Ser Ser Pro Leu His Arg Pro Gln Met Pro Phe Trp

180 185 190

Thr Cys Thr Phe Pro Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser

195 200 205

Thr Trp Gly Trp Arg Thr Phe Gly Pro Val Tyr Tyr Val Cys Asn Arg

210 215 220

Met Asn Val Ser Ser Val Leu Lys Pro Phe Leu Phe Thr Val Pro Ile

225 230 235 240

Glu Asp Met Arg Glu Cys Thr Leu Gly Arg Asn Arg Met Asn Val Ser

245 250 255

Ser Val Leu Lys Pro Cys Leu Ile Pro Val Pro Ile Leu Thr Thr Leu

260 265 270

Ser Phe Val Leu Ile Glu Glu Ser Arg Arg Asn Arg Arg Tyr Arg Lys

275 280 285

Ile Tyr Gly Glu Leu Lys Met Ser Leu Gln Ala Leu Pro Leu Thr Phe

290 295 300

628

Ser Thr Leu Leu Trp Asp Ser Arg His Thr Thr Thr Thr Ser Ser Pro

305 310 315 320

Arg Arg Ser Pro Ala Leu Arg Ala Arg Gly Gly Thr Thr Ile Gly Glu

325 330 335

Val Thr Ser Arg Gly Ala Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser

340 345 350

Phe Phe Thr Ser Ala His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr

355 360 365

Gln Ser Asn Gly Met Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu

370 375 380

Ser Arg Gly Pro Pro Leu His Thr Arg Thr Met Ser Leu Ser Asn Ile

385 390 395 400

Leu Met Lys Phe Arg Leu Met Leu Gln Lys Gln Cys Cys Tyr Asn Leu

405 410 415

Val Ile Lys His Pro Leu Ile Phe Arg Ile Asn Leu Arg Arg Gln Gly

420 425 430

Lys Thr Lys Asp Gly Val Val Gln Ser Leu Gly Phe Leu Asn Gln Gln

435 440 445

Ile Ile Leu Gln Lys Glu Phe Phe Ile Cys Leu Leu Met Phe Ile Lys

450 455 460

Arg Trp Lys Pro Ala Ala Thr Lys Thr Pro Pro Pro Leu Ile Leu Trp

465 470 475 480

Pro Gln Ala Ala Pro Val His Lys Lys Arg Gly Leu Phe Ser Leu Phe

485 490 495

Phe Ser His Cys Leu Cys His Gln Met Lys Cys Leu Thr Ala Val Asp

500 505 510

Cys Leu Gln Ala Phe Trp Asp Met Met Ile Arg Lys Ile Gln Lys Arg

515 520 525

Arg Arg Thr Ala Asn Arg Leu Lys Lys Lys Tyr Leu Val Gln Cys Thr

530 535 540

Asn Leu Ser Val Pro Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val

545 550 555 560

Phe Ile Leu Leu Leu Ser Asp Lys Lys Cys Leu Arg Val Arg Cys Gly

565 570 575

Gly Trp Leu Cys Gly Trp Thr Pro Arg Gly Lys Asn Lys Lys Arg Arg

580 585 590

Lys Leu Arg Pro Lys His Leu Lys Lys Thr Ser Ala Lys Val Ser Val

629

595 600 605

Ile Thr Met Lys Leu Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn

610 615 620

Leu Val Val Trp Ile Leu Asn Leu Lys Met Met Trp Leu Lys Lys Ser

625 630 635 640

Thr Lys Ser Arg Gln Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr

645 650 655

Met Gln Trp Asn Asn Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala

660 665 670

His His Cys Gln Tyr Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn

675 680 685

Trp Lys Lys Met Met Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu

690 695 700

Asp Ser Ser Pro Leu Gln Leu Leu Leu His Pro His Gly Lys Arg Cys

705 710 715 720

Asn Pro Cys Cys Arg Leu Thr Trp Met Ser Arg Glu Trp Glu Lys Arg

725 730 735

Lys Gln Ile Leu Gly Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu Lys

740 745 750

Arg Ile Leu Ile Arg Lys Ala Met Thr Val Leu Ala Gly Asp Lys Lys

755 760 765

Gly Leu Gly Arg Phe Met Arg Cys Val Gln Ser Glu Thr Lys Ala Val

770 775 780

Ser Leu Gln Leu Pro Leu Gly Arg Arg Lys Ser Gly Val Met Lys Cys

785 790 795 800

Trp Asp Leu Cys Pro Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly

805 810 815

Gly Glu Lys Leu Lys His Cys Phe Lys Ala Trp Gly Leu Cys Phe Phe

820 825 830

Tyr Leu Leu Tyr Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser Val Asp

835 840 845

Pro Pro Gly Ser Val Trp Ile Leu Pro Cys Met Asp Leu Asp Gly Met

850 855 860

Leu Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu

865 870 875 880

Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln Val

885 890 895

630

Gln Thr Gly Gly Arg Leu Phe Leu Ile His Leu Arg Lys Val Asp Thr

900 905 910

His His Leu Gln Val Val Thr Lys Thr Val Thr Ala Thr Thr Ala Asp

915 920 925

Gly Gln Arg Met Arg Arg Leu Lys Cys Lys Ala Cys Arg Leu Arg Trp

930 935 940

Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro Lys Ser His Phe Pro

945 950 955 960

Pro His Pro Ser Gly Pro Ser Arg Thr Ser Ser Leu Gln Leu Arg Pro

965 970 975

Ile Pro Pro His Leu Arg His Pro Leu Leu Leu Lys Gly Lys Asn Ser

980 985 990

Pro Cys Cys Gln Lys Lys Leu Arg Val Gln Asn Gln Gly His Leu Leu

995 1000 1005

Met Ile Leu Leu His Asn Ala Arg Gln Pro Ser Arg Pro Thr Gly

1010 1015 1020

Arg Thr Pro Thr Thr Arg Leu Met Ala Pro Val Gly Ser Val Met

1025 1030 1035

Ser Cys Ser Leu Leu Thr Gln Pro Ser Pro Ala Ser Ala Cys Thr

1040 1045 1050

Met Pro Pro Leu Leu His Pro Gln Trp Ala Pro His Ser Ala Gly

1055 1060 1065

Leu Ser Pro Gly Ala Cys Arg Pro Ala Cys Thr Cys Ile Ser Met

1070 1075 1080

Thr Thr Ile Ser Arg Ala Thr Trp Arg Leu Arg Ile Pro Ser Leu

1085 1090 1095

Arg Lys Tyr Arg Gln Asn Ile Glu Ile Thr Pro Gln Arg Ser Lys

1100 1105 1110

Ala Gln Val Ser Val Tyr Ser Gly Asn Leu Leu Ser Phe Ser Arg

1115 1120 1125

Arg Gly Met Lys Ser Ser Val Arg Leu Lys Asn Ser Ile Pro His

1130 1135 1140

Val Pro Lys Ala Thr Ala Ala His Ser Val Thr Val Lys Leu Gln

1145 1150 1155

Tyr Leu Phe Phe Leu Thr Val Cys Gly Phe Asp Arg Val Phe Leu

1160 1165 1170

631

Val Lys Ile Cys Lys Lys Lys Leu Leu Leu Lys Leu Leu Arg Gly

1175 1180 1185

Pro Arg Arg Lys Val Leu Met Val Leu Trp Leu Pro Ala Asn Gln

1190 1195 1200

Ser Leu Gln Lys Ser Gln Val Phe Gln Trp Val Leu Arg Thr Glu

1205 1210 1215

Thr Tyr Met Glu Met His Tyr Gly His Gln Ala Pro Gln Val Ala

1220 1225 1230

Met Leu Pro Lys Leu Leu Cys Arg Gln Met Leu Pro Thr Leu Ser

1235 1240 1245

Thr Leu Gln Gln Arg His Pro Gln Gly Pro Cys Pro Pro Pro Val

1250 1255 1260

Thr Pro Leu Arg Val Gln Ser Val Leu Leu Leu Gly Val Pro Gln

1265 1270 1275

Thr Ala Ala Phe Ser Gly Arg Ile Leu His Gln Pro Glu Met Ala

1280 1285 1290

Ala Met Arg Leu Met Pro Lys Phe Leu Asn Ser Thr Asn Ser Trp

1295 1300 1305

Trp Thr Val Ala Pro Arg Cys Pro Arg Ser Ala Val Asp Ile Ser

1310 1315 1320

Pro Gly Pro Thr Ser Pro Ser Leu Ala Ser Cys Ala Ser Val Ala

1325 1330 1335

Ile Gly Lys Leu Ala Leu Leu Gly Ala Ser Ala Pro Cys Gly Ala

1340 1345 1350

Ala Gly Ala Arg Glu Ala Gly Arg Phe Ala Val Leu Gly Thr Met

1355 1360 1365

Val Met Lys Gly Ala Ala Ala Ala Ser Cys Gln Ser Pro Pro Ser

1370 1375 1380

Pro Arg Glu Thr Thr Pro Ser Ser Asp Ala Thr Arg Arg Thr Arg

1385 1390 1395

Pro Ile Ala Ala Gln Ser Lys Lys Thr Met Thr Ala Ala Gly Leu

1400 1405 1410

Thr Pro Arg Arg Asn Leu Gln Arg Asn Leu Lys Val Leu Gly Ala

1415 1420 1425

Lys Lys Ile Thr Phe Leu Arg Asn Ser Arg Arg Ala Thr Pro Thr

1430 1435 1440

632

Ser Lys Gly Ser Leu Arg Arg Lys Tyr Leu Arg Val Asn Ile Arg

1445 1450 1455

Lys Leu Ile Trp Thr Lys Gln Arg Ser Phe Leu Ser Leu Phe Pro

1460 1465 1470

Lys Leu Arg Ala Thr Glu Gln Met Thr Asn Val Gly Cys Leu Leu

1475 1480 1485

Lys Lys Ser Ser Gln Leu Arg Arg Ser Trp Arg Asn Val Ser Arg

1490 1495 1500

Ile Ser Asn Met Glu His Arg Leu Thr Met Asp Ser Ala Leu Ser

1505 1510 1515

Leu Thr Lys Arg Glu Gly Pro Glu Thr Ser Glu Lys Lys Arg Lys

1520 1525 1530

Leu Pro Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1182

<211> 1547

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hTPA- and HA-extended polypeptide 4D poly-FSP

<400> 1182

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly

1 5 10 15

Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Asn Arg Lys

20 25 30

Thr Arg Lys Ala Ala Arg Glu Lys His Arg Lys Thr Asp Asp Lys Lys

35 40 45

Lys Lys Ser Thr Glu Lys Thr Lys Asn Ala Lys Lys Met Pro Phe Gln

50 55 60

Gln Arg Pro Pro Leu Gln Pro Val Asn Leu Asp Leu Gln His Gln Arg

65 70 75 80

Arg Arg Lys Lys Lys Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro

85 90 95

Lys Ala Asn Ser Thr Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn

100 105 110

Leu Leu Asn Leu Val Gln Leu Asn Pro His Ser Pro His His Arg Lys

115 120 125

Asp Glu Glu Asp His Gln Lys Arg His His His His Asn Gln Lys Lys

130 135 140

633

Met Ser Val Arg Ala Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr

145 150 155 160

Gly Val Arg Thr Arg Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro

165 170 175

Arg Val Pro Pro Ser Pro Pro Leu Ala Leu Gly Pro Arg Met Gln Leu

180 185 190

Cys Thr Gln Leu Ala Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His

195 200 205

Ile Leu Gly Pro Gln Arg His Thr Pro Gln Ala Pro Leu Arg Leu Trp

210 215 220

Ser Trp Cys Gly Thr Ser Gln Thr Tyr Phe Gly Ser Arg Pro Ser Pro

225 230 235 240

Cys Leu Ser Trp Ser Ser Arg Ile Val Ala Pro Gly His Ala Leu Ser

245 250 255

Cys Arg Thr Arg Arg Arg Trp Ser Ser Lys Arg Trp Ile Phe Ser Asn

260 265 270

Gln Leu Thr Val Gln Met Leu Cys Leu Pro Ile Thr His Pro Phe Ala

275 280 285

Ser Gln Pro Leu Gly Gln Glu Met Pro Asn Asn Thr Gln Ile Arg Leu

290 295 300

Leu Lys Pro Cys Arg Val Asn Ala Val Glu Glu Leu His Ser Arg Arg

305 310 315 320

Met Ser Val Arg Ile Val Lys Asn Trp Val Ser Cys Cys Tyr Val Arg

325 330 335

Leu Ser Ala Val Gly Leu Ser Thr Trp Ser Ala Leu Asp Glu Glu Lys

340 345 350

Lys Asn Gly Arg Ile Ser Ser Leu Lys Lys Lys Val Asn Ala Cys Ser

355 360 365

Ser Leu Ile Leu Ser Phe Gln Arg Val Lys Thr Gly Met Glu Ile Leu

370 375 380

Leu Trp Ile Leu Leu Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu

385 390 395 400

Gly Glu Met Gln Leu Cys Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp

405 410 415

Phe Ser Ala Pro Gln Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val

420 425 430

634

His Glu Leu Lys Lys Asn Leu Leu Leu Pro Met Cys Ser Ser Ser Leu Ala

435 440 445

Leu Pro Thr Gly Leu Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala

450 455 460

Val Leu Ile Ser Ser Ser Thr Ser His Phe Leu Met Arg Ser Pro Val

465 470 475 480

Leu Pro Ser Ser Arg Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met

485 490 495

Trp Thr Trp Ser Ser Trp Leu Lys Asn Glu Gly Asn Asn His Trp Pro

500 505 510

Arg Val Glu Met Pro Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg

515 520 525

Asn Thr Ala Gln Asn Pro Arg Leu Gln Gln His Leu Lys His Trp Gln

530 535 540

Gln Cys Thr Arg Ala Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr

545 550 555 560

Phe Phe His Phe Phe Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro

565 570 575

Gly Trp Ser Lys Gly Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser

580 585 590

Leu Ala Ser Gln Val Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp

595 600 605

Leu Ile Cys Phe Val Phe Phe Val Cys Val Glu Thr Gly Phe Cys His

610 615 620

Val Ala Gln Val Gly Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr

625 630 635 640

Asp Val Asn His Trp Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr

645 650 655

Gly Met Asn Arg Pro Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala

660 665 670

Arg Leu Ile Leu Ala Met Thr Leu Thr Leu Asn Gly Met Ala Lys Lys

675 680 685

Ser Gln Gln Pro Leu Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro

690 695 700

Phe Phe Arg Gly Ser Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys

705 710 715 720

Pro Ala Cys Thr Ser Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg

635

725 730 735

Ala Val Arg Arg Lys Gly Ser Pro Leu Trp Asp Cys Ser Thr Thr Thr

740 745 750

Gly Thr His His Gly Cys Leu Trp Glu Ile Pro Ser Val Thr Leu Leu

755 760 765

Ala His Pro Arg Thr Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys

770 775 780

Gly Ser Arg Thr Ser Cys Cys Pro Arg Ser His Pro Arg Leu Pro Arg

785 790 795 800

Thr Lys Trp Pro Leu Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly

805 810 815

Thr Leu Val Trp Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala

820 825 830

His Cys Pro Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro

835 840 845

Ser Ser Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly

850 855 860

Ala Val Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile

865 870 875 880

Gln Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser Trp

885 890 895

Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys Arg Trp

900 905 910

Pro Arg Arg Cys Gln Lys Lys Val Pro Ser Ala Ser Pro Trp Gly Leu

915 920 925

Val Leu Lys Lys Asn Arg Lys Gln Met Leu Asn Gln Lys Cys Leu Ala

930 935 940

Pro Phe Arg Glu Ser Thr Leu Gly Asn Phe Trp Met Lys Leu Leu Gln

945 950 955 960

Leu Pro Val Val Ile Asn Leu Lys Arg Leu Gln Lys Phe Asn Leu His

965 970 975

Leu Cys Tyr Tyr His Ser Gln Ser Asn Arg Asn Lys Ser Arg Gln Met

980 985 990

Glu Ser Leu Gly Met Lys Leu Gln Ser Gln Lys Ser Gln His His Cys

995 1000 1005

Ala Leu Arg Lys Ile Glu Trp Ala Thr Lys Leu Ser Leu Gly Gly

1010 1015 1020

636

Lys Ala Gly Pro Gln Ala Gln Glu Lys Ala Thr Ser Arg Ile Leu

1025 1030 1035

Asn Leu Gln Val Leu Lys Lys Ile Leu Arg Ser Phe Met Lys Leu

1040 1045 1050

Tyr His Ser Leu Val Met Cys Leu Arg Leu Arg Thr Lys Leu Glu

1055 1060 1065

Lys Ala Leu Ser Ala Leu Phe Ile Trp Pro Gln His Ser Tyr Lys

1070 1075 1080

Pro Leu Phe Phe His Gly Asp Leu Cys Glu Trp Gly Gly Gln Ala

1085 1090 1095

His Arg Gln Arg Asn Trp Leu Lys Asn Trp Lys Arg Phe Lys Leu

1100 1105 1110

Lys Lys Lys Tyr Ile Gln Lys Lys Lys Gln Lys Ser Asn Lys Ile

1115 1120 1125

Ile Cys Lys Gly His Arg Ile Thr Ile Ile Cys Ile Lys Asn Met

1130 1135 1140

Phe Leu Gln Arg Lys Gly Phe Leu Asn Leu Ser His Val Glu Lys

1145 1150 1155

Phe Cys Arg Gly Gly Pro Ser Ala Ser Asp Arg Cys Cys Ser Cys

1160 1165 1170

Ser Pro Ser Gly Phe Ser Ala Gly Arg Gly Arg Cys Pro Val Gln

1175 1180 1185

Gly Cys Leu Arg Pro His Arg Val Gln Leu Leu Arg Arg Trp Gly

1190 1195 1200

Pro Gly Ser Pro Ala Gly Gln Arg Leu Ser Lys Gly Phe Gln Leu

1205 1210 1215

Leu Arg Trp Trp Gly Pro Gly Ser Pro Ala Pro Glu Pro Arg Lys

1220 1225 1230

Gly Pro Phe Pro Pro Pro Asp Pro Pro Trp Pro Val Thr Ala Val

1235 1240 1245

Thr Val Met Ala Gly Ser Val Pro Ser Ala Gln Ser Val Asp Ala

1250 1255 1260

Leu Glu Ser Pro Gly Pro Leu Ala Leu Glu Gly Pro Ser Ser Pro

1265 1270 1275

Arg Asn Leu Leu Trp Arg Glu Met Ser Ile Phe Leu Pro Gly Ile

1280 1285 1290

637

Phe Thr Ala Lys Lys Ser Pro Thr Ser Phe Cys Cys Asn Ile Leu

1295 1300 1305

Asn Ser Leu Pro Ser Ser Met Glu Ile Ala Val Leu Gln Glu Asp

1310 1315 1320

Ser Lys Lys Met Thr Gln Lys Gly Gln Ala Phe Gly Ser Leu Cys

1325 1330 1335

Gln Leu Val Ser Ala Asp Phe Ser Ala Pro Gln Val Thr Arg Leu

1340 1345 1350

Arg Ser Leu Asn His Leu Leu Ile Thr Tyr Thr Pro His Ile Cys

1355 1360 1365

Arg His Thr Leu Leu Thr Pro Asp Thr Arg Trp Ile Phe Ser Asn

1370 1375 1380

Gln Leu Gln Thr Val Gln Met Leu Cys Leu Pro Pro Leu Lys Gln

1385 1390 1395

Gly Glu Arg Ile Ile Trp Pro Leu Gly Gly Phe Gln Ile Pro Asn

1400 1405 1410

Phe Phe Trp Ser Leu Gly Ile Gly His Thr Gly Ala Trp Trp His

1415 1420 1425

Thr Gly Arg Arg Arg Arg Glu Trp Gly Gly Ser Thr Val Ser Arg

1430 1435 1440

Ser Pro Leu Trp Ile Ser Ser Met Ile Tyr Leu Arg Gly Met Ala

1445 1450 1455

Phe Ala Ser Asp Ser Ser Ile Arg Thr Thr Arg Val Ser Trp Cys

1460 1465 1470

Arg Arg Cys Gly Ala Lys Ser Ala Ala Ala Ser Ser Ile Glu Ala

1475 1480 1485

Leu Thr Ala Leu Phe Thr Cys Arg Thr Ala Ser Ile Ser Val Asn

1490 1495 1500

Ile Ser Thr Leu Met Ser Ile Leu Glu Ala Leu Lys Lys Lys Pro

1505 1510 1515

Arg Asp Ile Cys Arg Ile Ser Cys Arg Cys Arg Arg Glu Tyr Arg

1520 1525 1530

Val Thr Met Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser

1535 1540 1545

<210> 1183

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 1D poly-FSP

<400> 1183

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Ala Asp Lys Lys Asn Val Arg Ser

225 230 235 240

Ala Ser Ser Pro Gly His Pro Leu Gly Pro Arg Arg Met Thr Arg Arg

245 250 255

Thr Met Thr Thr Thr Lys Met Met Met Met Arg Arg Thr Lys Arg Arg

260 265 270

639

Arg Arg Lys Arg Arg Arg Met Thr Met Met Thr Arg Arg Thr Leu Leu

275 280 285

Thr Lys Lys Thr Cys Leu Thr Pro Arg Thr Ser Ala Ala Thr Lys Ala

290 295 300

Leu Pro Arg Gln Thr Ile Ser Gln Arg Arg Pro Ile Gln Thr Pro Pro

305 310 315 320

Gly Thr Ser Leu Ala Ile Trp Gly Arg Arg Ser Lys Ile Glu Ala Ser

325 330 335

Pro Ile Gly Ser Ile Thr Pro Leu Ser Ile Ser Arg Pro Thr Ser Arg

340 345 350

Ala Arg Gln Ser Cys Cys Leu Pro Gly Leu Thr His Pro Ala His Gln

355 360 365

Pro Leu Gly Ser Met Val Gly Ala Gly Thr Gly Gly Gly Pro Ala Cys

370 375 380

Val Pro Val Ala Glu Ala Ser Thr Gly Ala Trp Thr Ala Glu Gly Ala

385 390 395 400

Ala Gly Thr Arg Cys Arg Ala Ser Arg Ala Pro Cys Arg Gln Leu Pro

405 410 415

Ala Ser Ser Pro Thr Lys Cys Glu Ile Cys Val Lys Arg Arg Arg Arg

420 425 430

Arg Lys Arg Ser Ser Gln Ser Arg Ser Pro Val Pro Lys Lys Arg Glu

435 440 445

Arg Met Met Leu Pro Val Arg Ala Arg Thr Ala Arg Ser Ala Leu Pro

450 455 460

Gln Gly Arg Ala Pro Leu Glu Gly Met Leu Leu Pro Arg Ala Leu Pro

465 470 475 480

Ser Leu Leu Ser Arg Met Pro Ala Arg Arg Glu Ser Arg Phe Trp Leu

485 490 495

Ala Leu Gln Ala Leu Gly Leu Arg Pro Pro His Leu Pro Ser Cys His

500 505 510

Pro Leu Pro Gln Leu Leu Gly Pro Pro Pro Pro His Gln Ala Pro Gly

515 520 525

Glu Lys Asn Asp Leu Gln Leu Phe Val Met Ser Asp Thr Thr Tyr Lys

530 535 540

Ile Tyr Trp Thr Val Ile Leu Leu Asn Pro Cys Gly Asn Leu His Leu

545 550 555 560

Lys Thr Thr Ser Leu Asn Ala Lys Lys Met Glu Asp Met Ile Trp Glu

565 570 575

Ser Asn Gln Gln Leu Lys Trp Thr Pro His Ile Leu Lys Ser Ala Ser

580 585 590

Ile Ala Lys Lys Arg Ile Lys Leu Arg Arg Asn Ser Thr Arg Arg Glu

595 600 605

Met Pro Ser Gln Ala Thr Pro Ser Pro Pro Gly Pro Trp Arg Ala Ala

610 615 620

Leu Gly Lys Glu Val Lys Arg Ser Gln Ser Lys Leu Arg Leu Arg Asn

625 630 635 640

Thr Ser Pro Phe Asp Lys Lys Gly Phe Val Ile Leu Ser Asn Met Gly

645 650 655

Gly Asn Arg Ser Ile Cys Leu Thr Ile Val Phe Phe Leu Ala Pro Tyr

660 665 670

Phe Pro Met Pro Ser Ser Trp Ser Ser Ser Gly Arg Thr Arg Leu Pro

675 680 685

Leu Arg Pro Gln Arg Gly Pro Arg Pro Leu His Arg Trp Leu Gln Gln

690 695 700

Thr Thr Gly Cys Ser Gln Arg Arg Gly Asp Cys Pro Arg Thr Met Ala

705 710 715 720

Gly Pro Ser Ala Ser Leu His Pro Ala Ser Gly Arg Ala Ser Arg Lys

725 730 735

Thr Trp Gln Arg Arg Leu Arg Ala Cys Arg Ala Gly Gly Pro Ala Gly

740 745 750

Trp Ala Arg Gln Ala Arg Lys Arg Lys Arg Glu Lys Arg Ser Gln Lys

755 760 765

Ser Arg Gln Asn His Leu Gln Leu Lys Ser Cys Arg Arg Lys Ile Ser

770 775 780

Asn Trp Ser Lys Leu Arg Ser Pro Trp Ile Phe Glu Ala Lys Lys Ser

785 790 795 800

Met Lys Arg Ser Gln Arg Pro Pro Arg Ser Ile Leu Ser Leu Phe Lys

805 810 815

Lys Lys Lys Pro Leu Thr Phe Ser Pro Ser Val Ser Lys Lys Arg Leu

820 825 830

Pro Lys Leu Lys Gly Lys Lys Arg Arg Lys Glu Ile Gln Arg Lys Val

835 840 845

Val Lys Asn Pro Ser Gln Pro Val Pro Leu Arg Asn Asp Gly Ile Thr

850 855 860

Asn Gly Asn Asp Ser Thr Arg Ser Cys Ser Ile Ser Leu Lys Lys Ser

865 870 875 880

Leu Glu Lys Met Pro Leu Met Arg Ser Leu Leu Tyr Ser Arg Met Cys

885 890 895

Leu Val Gln Ile Pro Thr His Thr Trp Glu Pro Asp Tyr Phe Val Ser

900 905 910

Leu Pro Met Met Phe Ser Trp Ala Thr Gln Ser Phe Pro Val Gly Ala

915 920 925

Val Phe Leu Thr Arg Pro Ala Ile Pro Cys Cys Ser Cys Thr Ser His

930 935 940

Ser Asn Pro Trp Ser Gln Ser Thr Leu Ala Ile Arg Phe Ile Ser Ala

945 950 955 960

Pro Gln Pro Ser Ser Ser Thr Val Thr Ala Ala Pro Ser Gly Gln Tyr

965 970 975

Phe Ala Leu Arg Ser Met Pro Lys Ser Arg Asn Gln Val Leu Phe Arg

980 985 990

Arg Asn Leu Thr Pro Ser Gln Leu Arg Thr Leu Ala Pro Pro Tyr Met

995 1000 1005

Leu Leu Pro Gln Ser Leu Ser Gln Ser Leu Ser Arg Lys Gln Ile

1010 1015 1020

Pro Ser Gln Ser Met Leu Val Arg Thr Arg Leu Thr Leu Thr Gly

1025 1030 1035

Thr Arg Ser Ser Gly Thr Arg Ala Ser Pro Ser Ala Val Cys Leu

1040 1045 1050

Cys Cys Trp Thr Cys Pro Ser Arg Ser Phe Pro Ala Ala Ser Pro

1055 1060 1065

Ser Gly Leu Arg Ala Pro Val Gly Ala Gln Ala Pro Pro Glu Val

1070 1075 1080

Thr Leu Arg Ala Ser Cys Ala Ser Ser Ala Ser Lys Ala Cys Ala

1085 1090 1095

Gly Leu Trp Pro Trp Gly Ala Gln Val Arg Met Lys Lys Lys Asp

1100 1105 1110

Ser Thr Ile Phe Ile Tyr Pro Arg Leu Lys Thr Phe Gly Val Pro

1115 1120 1125

Leu Gly Ser Ile Leu Cys Leu Ala Gly Ser Leu Ser Thr Met Met

1130 1135 1140

Ile Ile Ser Thr Thr Arg Gln Glu Cys Gly Arg Arg Ala Ser Val

1145 1150 1155

Pro Cys Arg Pro Met Ile Gly Ser Ala Arg Pro Gly Pro Trp Arg

1160 1165 1170

Thr Ser Ala Met Pro Ser Ala Met Gly Val Ala Leu Pro Thr Ser

1175 1180 1185

Cys Glu Ser Gly Arg Ser Pro Pro Ala Thr Gly Gly Arg Met Pro

1190 1195 1200

Gly Ser Gln Ser Ile Met Ser Trp Met Pro Pro Leu Ala Pro

1205 1210 1215

Cys Ala Ala Cys Pro Cys Ser Pro Ala Pro Thr Leu Cys Pro Ala

1220 1225 1230

His Pro Ala Arg Ala Pro Thr Ala Val Pro Ala Phe Thr Pro Leu

1235 1240 1245

Ser Ala His Pro Val Pro Val Leu Ser Gly Cys His Leu Ala Val

1250 1255 1260

Arg Thr Ser Met Leu Thr Leu Leu Pro Met Met Thr Leu Lys Ala

1265 1270 1275

Leu Val Lys Thr Trp Thr Gln Phe Arg Asn Tyr Pro Arg Arg His

1280 1285 1290

Pro His Arg Ser Pro Pro Thr Ser Cys Pro Pro Leu Leu Glu Ala

1295 1300 1305

Phe Pro Leu Pro Arg Ala Ser Leu Arg Ala Thr Val Pro Arg His

1310 1315 1320

Ser Ser Val Leu Ala Thr Gly Leu His Leu His Arg Gln Ile Ser

1325 1330 1335

Leu Pro Leu Arg Gly Phe Leu Leu His Gln Pro Leu Pro Gly Gln

1340 1345 1350

His Leu Trp Leu Ser His Arg Leu Arg Leu Arg Leu Pro Arg Thr

1355 1360 1365

Thr Glu Ile Gln Leu Thr Met Asn Asp Ser Lys His Lys Leu Glu

1370 1375 1380

Ser Pro Ala Leu Lys Gln Val Ser Pro Ala Ser Pro Pro Thr Gln

1385 1390 1395

Gln Pro Gln Thr Pro Gln Asp Ser Arg Gln Val Leu Ala Val Pro

1400 1405 1410

Met Ser Leu Ala Cys Ser Ile Thr Val Ser Phe Val Gly Gln Ile

1415 1420 1425

Ser Thr Leu Val Leu Asp Val Ser Ser Ile Ser His Trp Asn Gly

1430 1435 1440

Ile Trp Glu Val Met Pro Arg Thr Leu Glu Gly Cys Ile Ser Val

1445 1450 1455

Lys Asp His Arg Thr Ser Gln Asn His Ser Lys Cys Ile Thr Glu

1460 1465 1470

Cys Val Arg Thr Ala Leu Asn Thr Lys Cys Glu Ile Cys Val Lys

1475 1480 1485

Asn Thr Ala Ile Leu Ala Ile Thr Leu Ile Thr Pro Gln Asp Pro

1490 1495 1500

His Leu Asp Pro Val Cys His Pro His Arg Arg Pro Gly Trp Lys

1505 1510 1515

Gly His His Arg Val Val Phe Ser His His Pro Ser Gly Ser Thr

1520 1525 1530

Lys Ser His Trp Gln Gln Arg Glu Arg Gly Gly Gly Arg Arg Glu

1535 1540 1545

Lys Lys Gly Cys Arg Glu Ala Ser Arg Ala Gly Gln Phe Pro Val

1550 1555 1560

Trp Arg Arg Thr Ser Arg Gly Met Ser Leu Gln Trp Lys Ser Gly

1565 1570 1575

Asn Ala Cys Thr Pro Leu Arg Cys Pro Ser Arg Ser Pro Ala Thr

1580 1585 1590

Phe Leu Thr Ala Leu Asn Gly Trp His Arg Ser Gly Leu Arg Ala

1595 1600 1605

Gln Thr Ala Phe Phe Leu Leu Asp Ile Ile Leu Arg Arg Ala Arg

1610 1615 1620

Pro Thr Ser Cys Ser Thr Cys Arg Met Trp Trp Arg Leu Pro Cys

1625 1630 1635

Lys Val Thr Glu Ser Ser Leu Ala Lys Lys Thr Leu Thr Asn Gln

1640 1645 1650

Pro Ser Leu Pro Gly Pro Cys Ala Ser Leu Leu Ser Thr Leu Ser

1655 1660 1665

Gln Pro Pro Pro Gln Ala Pro Ser Gln Val Trp Thr Ala Ala Thr

1670 1675 1680

Leu Arg Cys Pro Ala Val Pro Ala Ala Ala Cys Pro Pro Leu Lys

1685 1690 1695

Leu Ala Pro Leu Gln Val Thr Thr Thr Leu Lys Leu Ile Leu Val

1700 1705 1710

Met Leu Glu Ile Val Thr Glu Asn Lys Val Arg Gln Asn Phe Lys

1715 1720 1725

Arg Asn Ser Ser Phe Phe Tyr Thr Ser Asp Arg Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1184

<211> 1744

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 2D poly-FSP

<400> 1184

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Arg Lys Met Gln Ser Leu Asn His

225 230 235 240

Pro Ser Arg Leu Thr Asn Asn Pro Arg Arg Lys Thr Trp Lys Met Arg

245 250 255

Lys Lys Tyr Ala Gln Lys Pro Ser Gln Lys Asn His Leu Tyr Arg Gln

260 265 270

Ile Asn Arg Arg Lys Leu Gln Arg Lys Lys Ser Arg Ser His Leu Asp

275 280 285

Phe Ser Pro Tyr Trp Met Lys Lys Val Lys Arg Ala Arg Asn Arg Asn

290 295 300

Met Lys Lys Thr Met Ser His Leu Ile Ala His Gln Ala Leu Lys Met

305 310 315 320

Ser Gly Leu Arg Leu Phe His Pro Arg Leu Leu Thr Arg Lys Lys Pro

325 330 335

Gly Lys Gln Lys Lys Gly His Met Ile Phe Ser Arg Arg Lys Trp Gln

340 345 350

Ile Arg His Val Leu Lys Asn Leu Ile Pro Ser Leu Asn Ala Gln Trp

355 360 365

Met Phe Leu Arg Ile Ala Ser Pro Gln Ile Met Gln Arg Thr Ser Ala

370 375 380

Asp Arg Phe Arg Met Lys Arg Arg Met Gln Asn Leu Asp Asn Leu Gly

385 390 395 400

Val Pro Trp Pro Leu Leu Pro Gly Leu Leu Leu Ala His Met Tyr Gly

405 410 415

Phe Ser Thr Tyr Gly Thr Gln Lys Gly Glu Cys Val Cys Leu Gln Cys

420 425 430

Leu Ser Gly Ser Ser Leu Phe Ile Leu Lys Gln Pro Leu Asp Leu Lys

435 440 445

Ile Ser Lys Thr Phe Met Ala Ser Pro Gln Ile Met Gln Arg Met Ser

450 455 460

Ala Val Asn Phe Glu Thr Ile Leu Gln Ala His Pro Gln Val Pro Ala

465 470 475 480

Leu Ala Pro Gln Ala Trp Trp Pro Ala Arg Arg Gly Pro Leu Thr Ser

485 490 495

Ala Pro Ser Ala Gln Gln Ser Leu Thr Arg Thr Leu Glu Ser Ala Gly

500 505 510

Leu Phe Pro Trp Leu His Thr Gln Cys Arg Ala Phe Ile His Thr Pro

515 520 525

Arg Ser Pro Gly Cys Ser Val Tyr Ala Asn Met Cys Trp Arg Tyr Lys

530 535 540

Lys Arg Gly Val Ile Ala Trp Arg Ile Tyr Pro Pro Ser Thr Cys Ser

545 550 555 560

Leu Pro Cys Arg Gly Leu Ile Leu Ile Gln His Gly Gly Ile Phe Thr

565 570 575

Phe Arg Phe Trp Val Glu Lys Leu Ser Trp Asn Ile Cys Lys Asn Leu

580 585 590

Ser Leu Tyr Leu Asp Lys Phe Val Gln Arg Leu Leu Tyr Val Tyr Ile

595 600 605

Ile Glu Thr Asn Val Phe Val Leu Asn Leu Phe His Val Pro Val Asn

610 615 620

Gln Ile Phe Met Met Ala Phe Tyr Leu Lys Tyr Thr Glu Lys Ala Trp

625 630 635 640

Val Met Glu Leu Glu Trp Leu Ile Gln Gln Gln Cys Tyr Gln Arg Lys

645 650 655

Arg Met Lys Thr Gly Ala Ile Gln Glu Lys Gly Ala Ile Gln Glu Lys

660 665 670

Gly Ala Met Thr Lys Arg Ile Cys Val Val Asn Phe Glu Ala Cys Arg

675 680 685

Glu Met Glu Ser Leu Ser Ala Pro Glu Lys Ile Thr Leu Phe Glu Ala

690 695 700

His Met Ala Arg Cys Thr Ser Ile Asn Val Leu Cys Val Arg Ala Ser

705 710 715 720

Leu Ile Glu Lys Leu Met Lys Glu Lys Arg Lys Met Lys Arg Asn Gln

725 730 735

Val Ala Ser Pro Gln Ile Met Gln Arg Leu Phe Ile Lys Gly Tyr Gln

740 745 750

Asn Asn His Phe Thr Phe His Leu Gln Asn Phe Ala Phe Phe Ser Ser

755 760 765

Phe Gly Asn Asn Lys Tyr Ile Asn Ile Leu Thr Phe Leu Pro Ser Arg

770 775 780

Phe Pro Trp Gly Pro Pro Pro Leu Gly Ala Leu Arg Ala Thr Ala Pro

785 790 795 800

Ala Thr Ala Ala Pro Gln Ala Gln Thr Pro His Trp Arg Ala Ala Thr

805 810 815

Thr Ser Ser Met Ser Ser Ser Pro Arg Ala Glu Pro Thr Ser Ser Ser

820 825 830

Ser Trp Ala Thr Cys Trp Pro Arg Cys Cys Ser Ser Ser Ser Cys Tyr Trp

835 840 845

Ser Leu Ser Ser Trp Pro Pro Ala Gly Ala Ala Glu Ala Thr Asn Thr

850 855 860

Arg Thr Arg Ser Arg Glu Ser Gln Arg Gly Arg Met Leu Thr Trp Arg

865 870 875 880

Ser Ser Leu Trp Leu Gln Gly Thr Arg Cys Phe Thr Gly Val Arg Thr

885 890 895

Ser Ser Arg Cys Ser Leu Met Glu Gln Ile Pro His Leu Lys Ser Asn

900 905 910

Ser Arg Lys Lys Leu Leu Glu Asp Met Lys Gln Asn Gly Phe Thr Arg

915 920 925

Arg Arg Asn Ser Arg Lys Gln Ser Gln Val Gln Leu Ala Ile Gln Val

930 935 940

Leu Leu Val Asp Lys His Lys Tyr Phe Leu Phe Gly Arg Ala Thr Phe

945 950 955 960

Leu Leu Ala Leu Trp Glu Cys Ser Leu Pro Gln Ala Arg Leu Cys Leu

965 970 975

Ile Val Ser Arg Thr Leu Leu Leu Val Gln Ser Arg His Pro Ser Trp

980 985 990

Pro Trp Thr Arg Cys Leu Arg Met Arg Pro Pro Arg Ser His Trp Asn

995 1000 1005

Ser Ala Tyr Leu Gly Arg Thr Leu Leu Val Thr Cys Ile Leu Tyr

1010 1015 1020

His Arg Trp Ile Val Thr Ser Met Cys Gln Ser Gln Arg Trp Leu

1025 1030 1035

Thr Ser Thr Thr Ser Arg Ser Ser Ala Leu Met Trp Thr Trp Trp

1040 1045 1050

Cys Thr Arg Gly Gly Gly Lys Arg Lys Lys Lys Met Cys Lys Val

1055 1060 1065

Ser Gly Gly Cys Thr Ala Cys Val Glu Arg Cys Pro Trp Lys Gly

1070 1075 1080

Ile Cys Trp Lys Trp Pro Val Thr Val Ala Ala Met Ser Arg Thr

1085 1090 1095

Pro Pro Arg Arg Val Cys Gly His Arg Glu Gly Ser Glu Ser Asp

1100 1105 1110

Asn Leu Gly Leu Leu Tyr Ser Phe Pro Pro Val Leu Phe Lys Ala

1115 1120 1125

Asp Gln Ser Glu Ser Ser Leu Ser Ser Gly Asn Ser Val Ile Val

1130 1135 1140

His Lys Ile Val Leu Val Phe Leu Lys Ser Leu Ser Ser Tyr Asn

1145 1150 1155

Cys Glu Arg Arg Ile Ser Gly Ile Gln Lys Arg Ala Ile Glu Phe

1160 1165 1170

Ala Val Leu Lys Leu Leu Asn Met Asp Leu Gly Lys Gly Leu Thr

1175 1180 1185

His Pro Val Pro Leu Lys Arg Thr Leu Gln Asn Lys Arg Asn Lys

1190 1195 1200

Asn Ile Lys Arg Val Asn Gln Lys Lys Gly Asn Ile Lys Arg Lys

1205 1210 1215

Ser Ile Leu Leu His Leu Ile Val Leu Asn Ser Pro Glu Ser Asn

1220 1225 1230

Arg Glu Lys Ile Ile Asn Pro Thr Ile Ser Cys Pro Phe Gln Ser

1235 1240 1245

Pro Thr Lys Arg Leu Gly Ser Pro Pro Leu His Leu Cys Gln Pro

1250 1255 1260

Leu Arg Pro Tyr Pro Ile Ser Glu Gly Leu Thr Met Thr Gly

1265 1270 1275

Arg Asn Ile Asn Ser Asn Ser Leu Met Thr Asn Cys Leu Phe Ser

1280 1285 1290

Pro Ile Phe Leu Cys His His Ala Ile Met His Arg Lys Met Thr

1295 1300 1305

Ser Pro His Phe Arg Leu Phe Ser Ser Lys Ile Pro His Pro Gln

1310 1315 1320

Val Pro Ser Val Val Ala Leu Cys Lys Phe Thr His Pro Cys Thr

1325 1330 1335

Met Leu Leu Pro Gln Gly Ala Phe Trp Lys Pro Pro Ser Gln Gly

1340 1345 1350

Ile Ser Ser Cys Leu Met Thr Gly Cys Trp Gln Trp Ser Ser Val

1355 1360 1365

Thr Ser Leu Glu Trp Thr Ala Gln Glu Arg Gly Cys Ser Ser Trp

1370 1375 1380

Leu Met Lys Gln Thr Trp Met Lys Ser Trp Ser Leu Arg Asp Pro

1385 1390 1395

Ser Tyr Arg Ser Ile Leu Glu Tyr Val Ser Thr Arg Val Leu Trp

1400 1405 1410

Met Pro Thr Ser Thr Val Pro Lys Phe Phe Leu Thr Phe Ser Gln

1415 1420 1425

Ile Arg Met Cys Ala Leu Ser His Pro Ser Phe Tyr Ile Ser Ser

1430 1435 1440

Thr Val Cys Phe Leu Ser Lys His Cys Met Thr Ala Cys Thr Pro

1445 1450 1455

Gly Thr Ser Leu Gln Cys Tyr Leu Asn Leu Ala Asn Gly Leu Trp

1460 1465 1470

Phe Asn Leu Ile Phe Arg Asn Lys Lys Thr Lys Leu Trp Phe Ser

1475 1480 1485

Leu Ile Asn Ile His His Arg Lys Asn Pro Leu Leu Pro Met Arg

1490 1495 1500

Leu Glu Lys Lys Ala Thr Trp Met Arg Arg Thr Glu Leu Ser Leu

1505 1510 1515

Glu Pro Trp Ile Pro Tyr Leu His Gln Gln Lys Thr Gln Trp Leu

1520 1525 1530

Pro Met Arg Ser Leu Gly Glu Pro Trp Arg Ala Gly Phe Gln Trp

1535 1540 1545

Gly Glu Ser Pro Trp Gln Val Met Ala Ser Leu Thr Gly Arg Arg

1550 1555 1560

Lys Lys Arg Glu Asp His Cys Gly Leu Gln Glu Lys Lys Ser Arg

1565 1570 1575

Lys Leu Met Ser Ser Cys Ala Ala Pro Pro His Arg Leu Leu Gly

1580 1585 1590

Pro Ser Arg Trp Gly Ser Cys Gly Arg Ala Cys Ser Cys Gly Arg

1595 1600 1605

Gly Cys Gly Thr Pro Arg Gly Asp Trp Lys Arg His Pro Pro Arg

1610 1615 1620

Arg Ile Leu Phe His Val Arg Asn Ile Val Tyr Gly Ile Gln Val

1625 1630 1635

Met Leu Cys Cys His Pro Ala Trp Leu Ser Pro Ile Ser Ala Cys

1640 1645 1650

Pro Arg Leu Leu Pro Tyr Pro Phe His Val Leu Ala Leu Glu Val

1655 1660 1665

Thr Phe Pro Pro Gly Thr Ala Met Pro Gln Met Gln Leu Gln Ala

1670 1675 1680

Leu Ala Met Trp Glu Lys Gly Trp Asp Lys Pro Leu Glu Asn Thr

1685 1690 1695

Gln Ile Pro Arg Thr Ser Pro Leu Trp Lys Ile Thr Met Val Leu

1700 1705 1710

Thr Tyr Pro Asn Cys Val Val His Val Leu Met Phe Gly Gly Lys

1715 1720 1725

Lys Arg Ser Lys Phe Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala

1730 1735 1740

Ser

<210> 1185

<211> 1749

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 3D poly-FSP

<400> 1185

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Gln Asn Thr Arg Ser Thr Asn Leu

225 230 235 240

Glu Lys Asn Pro Ser Leu Lys Lys Gln Ser Gln Arg Arg Gly Lys Leu

245 250 255

Ser Ser Arg Lys Lys Gly His Thr Lys Asn Arg Arg Lys Ala Lys Arg

260 265 270

Lys Pro Gln Ile Ser Gln Lys Lys Asp Pro Ala Gly Lys Leu Lys Lys

275 280 285

His Leu Asn Arg Lys Leu His Leu Thr Arg Ser Cys Leu Ile Asp Leu

290 295 300

Thr Met Met Met Ser Gln Lys Leu Trp Lys Asn Gln Arg Lys Arg Ile

305 310 315 320

Pro Leu Pro Ser Pro Arg Gln His Leu Leu Pro Gln Tyr Pro Leu His

325 330 335

Pro Pro Pro Pro Cys Leu Leu Leu Leu His His Arg His His Leu Ala

340 345 350

Phe Leu Glu Met Ala Cys Ser Ser Gln His Thr His Ser Ile Lys Ile

355 360 365

Trp Ile Ser Phe Ser His Glu Asp Leu Pro Arg Met Leu Pro Ile Ser

370 375 380

Ser Pro Leu His Arg Pro Gln Met Pro Phe Trp Thr Cys Thr Phe Pro

385 390 395 400

Ala Thr Thr Trp Gly Thr Trp Glu Thr Pro Ser Thr Trp Gly Trp Arg

405 410 415

Thr Phe Gly Pro Val Tyr Tyr Val Cys Asn Arg Met Asn Val Ser Ser

420 425 430

Val Leu Lys Pro Phe Leu Phe Thr Val Pro Ile Glu Asp Met Arg Glu

435 440 445

Cys Thr Leu Gly Arg Asn Arg Met Asn Val Ser Ser Val Leu Lys Pro

450 455 460

Cys Leu Ile Pro Val Pro Ile Leu Thr Thr Leu Ser Phe Val Leu Ile

465 470 475 480

Glu Glu Ser Arg Arg Asn Arg Arg Tyr Arg Lys Ile Tyr Gly Glu Leu

485 490 495

Lys Met Ser Leu Gln Ala Leu Pro Leu Thr Phe Ser Thr Leu Leu Trp

500 505 510

Asp Ser Arg His Thr Thr Thr Thr Ser Ser Pro Arg Arg Ser Pro Ala

515 520 525

Leu Arg Ala Arg Gly Gly Thr Thr Ile Gly Glu Val Thr Ser Arg Gly

530 535 540

Ala Ser Ser Ser Thr Ser Arg Asp Ser Arg Ser Phe Phe Thr Ser Ala

545 550 555 560

His Pro Ser Arg Pro Cys Arg Met Arg Leu Tyr Gln Ser Asn Gly Met

565 570 575

Glu Ala Cys Gly Thr His Pro Thr Pro Arg Leu Ser Arg Gly Pro Pro

580 585 590

Leu His Thr Arg Thr Met Ser Leu Ser Asn Ile Leu Met Lys Phe Arg

595 600 605

Leu Met Leu Gln Lys Gln Cys Cys Tyr Asn Leu Val Ile Lys His Pro

610 615 620

Leu Ile Phe Arg Ile Asn Leu Arg Arg Gln Gly Lys Thr Lys Asp Gly

625 630 635 640

Val Val Gln Ser Leu Gly Phe Leu Asn Gln Gln Ile Ile Leu Gln Lys

645 650 655

Glu Phe Phe Ile Cys Leu Leu Met Phe Ile Lys Arg Trp Lys Pro Ala

660 665 670

Ala Thr Lys Thr Pro Pro Pro Leu Ile Leu Trp Pro Gln Ala Ala Pro

675 680 685

Val His Lys Lys Arg Gly Leu Phe Ser Leu Phe Phe Ser His Cys Leu

690 695 700

Cys His Gln Met Lys Cys Leu Thr Ala Val Asp Cys Leu Gln Ala Phe

705 710 715 720

Trp Asp Met Met Ile Arg Lys Ile Gln Lys Arg Arg Arg Thr Ala Asn

725 730 735

Arg Leu Lys Lys Lys Tyr Leu Val Gln Cys Thr Asn Leu Ser Val Pro

740 745 750

Met Met Leu Thr Ile Leu Ile Trp Lys Arg Val Phe Ile Leu Leu Leu

755 760 765

Ser Asp Lys Lys Cys Leu Arg Val Arg Cys Gly Gly Trp Leu Cys Gly

770 775 780

Trp Thr Pro Arg Gly Lys Asn Lys Lys Arg Arg Lys Leu Arg Pro Lys

785 790 795 800

His Leu Lys Lys Thr Ser Ala Lys Val Ser Val Ile Thr Met Lys Leu

805 810 815

Trp Glu Lys His Phe Lys Gly Leu Asn Trp Asn Leu Val Val Trp Ile

820 825 830

Leu Asn Leu Lys Met Met Trp Leu Lys Lys Ser Thr Lys Ser Arg Gln

835 840 845

Ser Ser Pro Phe Leu Ala Arg Ser Trp Asn Thr Met Gln Trp Asn Asn

850 855 860

Lys Lys Met Ala Met Thr Val Ile Ala Gly Ala His His Cys Gln Tyr

865 870 875 880

Lys Leu Thr Tyr Thr Lys Val Trp Lys Met Asn Trp Lys Lys Met Met

885 890 895

Ile Gly Ser Gln His Leu Leu Phe Ser Pro Leu Asp Ser Ser Pro Leu

900 905 910

Gln Leu Leu Leu His Pro His Gly Lys Arg Cys Asn Pro Cys Cys Arg

915 920 925

Leu Thr Trp Met Ser Arg Glu Trp Glu Lys Arg Lys Gln Ile Leu Gly

930 935 940

Gly Gly Gly Lys Tyr Lys Glu Tyr Phe Leu Lys Arg Ile Leu Ile Arg

945 950 955 960

Lys Ala Met Thr Val Leu Ala Gly Asp Lys Lys Gly Leu Gly Arg Phe

965 970 975

Met Arg Cys Val Gln Ser Glu Thr Lys Ala Val Ser Leu Gln Leu Pro

980 985 990

Leu Gly Arg Arg Lys Ser Gly Val Met Lys Cys Trp Asp Leu Cys Pro

995 1000 1005

Arg Leu Asp Phe His Pro Arg Ser Ser Ser Gly Gly Glu Lys Leu

1010 1015 1020

Lys His Cys Phe Lys Ala Trp Gly Leu Cys Phe Phe Tyr Leu Leu

1025 1030 1035

Tyr Phe Glu Lys Lys Arg Pro Glu Trp Leu Ser Val Asp Pro Pro

1040 1045 1050

Gly Ser Val Trp Ile Leu Pro Cys Met Asp Leu Asp Gly Met Leu

1055 1060 1065

Thr Val Ile Gly Gln Gly Leu Val Gly Met Thr Pro Arg Asp Leu

1070 1075 1080

Gly Thr Val Ala Gly Ala Thr Ala Gln Ile Gln Arg Pro Ile Gln

1085 1090 1095

Val Gln Thr Gly Gly Arg Leu Phe Leu Ile His Leu Arg Lys Val

1100 1105 1110

Asp Thr His His Leu Gln Val Val Thr Lys Thr Val Thr Ala Thr

1115 1120 1125

Thr Ala Asp Gly Gln Arg Met Arg Arg Leu Lys Cys Lys Ala Cys

1130 1135 1140

Arg Leu Arg Trp Ala Arg Pro Glu Pro Ala Ala Gln Ala Pro Pro

1145 1150 1155

Lys Ser His Phe Pro Pro His Pro Ser Gly Pro Ser Arg Thr Ser

1160 1165 1170

Ser Leu Gln Leu Arg Pro Ile Pro Pro His Leu Arg His Pro Leu

1175 1180 1185

Leu Leu Lys Gly Lys Asn Ser Pro Cys Cys Gln Lys Lys Leu Arg

1190 1195 1200

Val Gln Asn Gln Gly His Leu Leu Met Ile Leu Leu His Asn Ala

1205 1210 1215

Arg Gln Pro Ser Arg Pro Thr Gly Arg Thr Pro Thr Thr Arg Leu

1220 1225 1230

Met Ala Pro Val Gly Ser Val Met Ser Cys Ser Leu Leu Thr Gln

1235 1240 1245

Pro Ser Pro Ala Ser Ala Cys Thr Met Pro Pro Leu Leu His Pro

1250 1255 1260

Gln Trp Ala Pro His Ser Ala Gly Leu Ser Pro Gly Ala Cys Arg

1265 1270 1275

Pro Ala Cys Thr Cys Ile Ser Met Thr Thr Ile Ser Arg Ala Thr

1280 1285 1290

Trp Arg Leu Arg Ile Pro Ser Leu Arg Lys Tyr Arg Gln Asn Ile

1295 1300 1305

Glu Ile Thr Pro Gln Arg Ser Lys Ala Gln Val Ser Val Tyr Ser

1310 1315 1320

Gly Asn Leu Leu Ser Phe Ser Arg Arg Gly Met Lys Ser Ser Val

1325 1330 1335

Arg Leu Lys Asn Ser Ile Pro His Val Pro Lys Ala Thr Ala Ala

1340 1345 1350

His Ser Val Thr Val Lys Leu Gln Tyr Leu Phe Phe Leu Thr Val

1355 1360 1365

Cys Gly Phe Asp Arg Val Phe Leu Val Lys Ile Cys Lys Lys Lys

1370 1375 1380

Leu Leu Leu Lys Leu Leu Arg Gly Pro Arg Arg Lys Val Leu Met

1385 1390 1395

Val Leu Trp Leu Pro Ala Asn Gln Ser Leu Gln Lys Ser Gln Val

1400 1405 1410

Phe Gln Trp Val Leu Arg Thr Glu Thr Tyr Met Glu Met His Tyr

1415 1420 1425

Gly His Gln Ala Pro Gln Val Ala Met Leu Pro Lys Leu Leu Cys

1430 1435 1440

Arg Gln Met Leu Pro Thr Leu Ser Thr Leu Gln Gln Arg His Pro

1445 1450 1455

Gln Gly Pro Cys Pro Pro Pro Val Thr Pro Leu Arg Val Gln Ser

1460 1465 1470

Val Leu Leu Leu Gly Val Pro Gln Thr Ala Ala Phe Ser Gly Arg

1475 1480 1485

Ile Leu His Gln Pro Glu Met Ala Ala Met Arg Leu Met Pro Lys

1490 1495 1500

Phe Leu Asn Ser Thr Asn Ser Trp Trp Thr Val Ala Pro Arg Cys

1505 1510 1515

Pro Arg Ser Ala Val Asp Ile Ser Pro Gly Pro Thr Ser Pro Ser

1520 1525 1530

Leu Ala Ser Cys Ala Ser Val Ala Ile Gly Lys Leu Ala Leu Leu

1535 1540 1545

Gly Ala Ser Ala Pro Cys Gly Ala Ala Gly Ala Arg Glu Ala Gly

1550 1555 1560

Arg Phe Ala Val Leu Gly Thr Met Val Met Lys Gly Ala Ala Ala

1565 1570 1575

Ala Ser Cys Gln Ser Pro Pro Ser Pro Arg Glu Thr Thr Pro Ser

1580 1585 1590

Ser Asp Ala Thr Arg Arg Thr Arg Pro Ile Ala Ala Gln Ser Lys

1595 1600 1605

Lys Thr Met Thr Ala Ala Gly Leu Thr Pro Arg Arg Asn Leu Gln

1610 1615 1620

Arg Asn Leu Lys Val Leu Gly Ala Lys Lys Ile Thr Phe Leu Arg

1625 1630 1635

Asn Ser Arg Arg Ala Thr Pro Thr Ser Lys Gly Ser Leu Arg Arg

1640 1645 1650

Lys Tyr Leu Arg Val Asn Ile Arg Lys Leu Ile Trp Thr Lys Gln

1655 1660 1665

Arg Ser Phe Leu Ser Leu Phe Pro Lys Leu Arg Ala Thr Glu Gln

1670 1675 1680

Met Thr Asn Val Gly Cys Leu Leu Lys Lys Ser Ser Gln Leu Arg

1685 1690 1695

Arg Ser Trp Arg Asn Val Ser Arg Ile Ser Asn Met Glu His Arg

1700 1705 1710

Leu Thr Met Asp Ser Ala Leu Ser Leu Thr Lys Arg Glu Gly Pro

1715 1720 1725

Glu Thr Ser Glu Lys Lys Arg Lys Leu Pro Gly Tyr Pro Tyr Asp

1730 1735 1740

Val Pro Asp Tyr Ala Ser

1745

<210> 1186

<211> 1750

<212> PROTEIN

<213> Artificial sequence

<220>

<223> hINV - and HA-extended polypeptide 4D poly-FSP

<400> 1186

Met His Arg Arg Arg Ser Arg Ser Cys Arg Glu Asp Gln Lys Pro Val

1 5 10 15

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met

20 25 30

Leu Gly Arg Arg Pro Gly Ala Pro Glu Ser Lys Cys Ser Arg Gly Ala

35 40 45

Leu Tyr Thr Gly Phe Ser Ile Leu Val Thr Leu Leu Leu Ala Gly Gln

50 55 60

Ala Thr Thr Ala Tyr Phe Leu Tyr Gln Gln Gln Gly Arg Leu Asp Lys

65 70 75 80

Leu Thr Val Thr Ser Gln Asn Leu Gln Leu Glu Asn Leu Arg Met Lys

85 90 95

Leu Pro Lys Pro Pro Lys Pro Val Ser Lys Met Arg Met Ala Thr Pro

100 105 110

Leu Leu Met Gln Ala Leu Pro Met Gly Ala Leu Pro Gln Gly Pro Met

115 120 125

Gln Asn Ala Thr Lys Tyr Gly Asn Met Thr Glu Asp His Val Met His

130 135 140

Leu Leu Gln Asn Ala Asp Pro Leu Lys Val Tyr Pro Pro Leu Lys Gly

145 150 155 160

Ser Phe Pro Glu Asn Leu Arg His Leu Lys Asn Thr Met Glu Thr Ile

165 170 175

Asp Trp Lys Val Phe Glu Ser Trp Met His His Trp Leu Leu Phe Glu

180 185 190

Met Ser Arg His Ser Leu Glu Gln Lys Pro Thr Asp Ala Pro Pro Lys

195 200 205

Glu Ser Leu Glu Leu Glu Asp Pro Ser Ser Gly Leu Gly Val Thr Lys

210 215 220

Gln Asp Leu Gly Pro Val Pro Met Asn Arg Lys Thr Arg Lys Ala Ala

225 230 235 240

Arg Glu Lys His Arg Lys Thr Asp Asp Lys Lys Lys Lys Ser Thr Glu

245 250 255

Lys Thr Lys Asn Ala Lys Lys Met Pro Phe Gln Gln Arg Pro Pro Leu

260 265 270

Gln Pro Val Asn Leu Asp Leu Gln His Gln Arg Arg Arg Lys Lys Lys

275 280 285

Lys Asp Lys Val Glu Ile Arg Asn Arg Ser Pro Lys Ala Asn Ser Thr

290 295 300

Glu Cys Gln Gly Glu His Ser Arg Glu Gln Asn Leu Leu Asn Leu Val

305 310 315 320

Gln Leu Asn Pro His Ser Pro His His Arg Lys Asp Glu Glu Asp His

325 330 335

Gln Lys Arg His His His His Asn Gln Lys Met Ser Val Arg Ala

340 345 350

Ser Val Ser Phe Trp Thr Leu Leu Pro Thr Thr Gly Val Arg Thr Arg

355 360 365

Gly Ser Pro Leu Arg Leu Thr Leu Trp Pro Pro Arg Val Pro Pro Ser

370 375 380

Pro Pro Leu Ala Leu Gly Pro Arg Met Gln Leu Cys Thr Gln Leu Ala

385 390 395 400

Arg Phe Phe Pro Ile Thr Pro Pro Val Trp His Ile Leu Gly Pro Gln

405 410 415

Arg His Thr Pro Gln Ala Pro Leu Arg Leu Trp Ser Trp Cys Gly Thr

420 425 430

Ser Gln Thr Tyr Phe Gly Ser Arg Pro Ser Pro Cys Leu Ser Trp Ser

435 440 445

Ser Arg Ile Val Ala Pro Gly His Ala Leu Ser Cys Arg Thr Arg Arg

450 455 460

Arg Trp Ser Ser Lys Arg Trp Ile Phe Ser Asn Gln Leu Thr Val Gln

465 470 475 480

Met Leu Cys Leu Pro Ile Thr His Pro Phe Ala Ser Gln Pro Leu Gly

485 490 495

Gln Glu Met Pro Asn Asn Thr Gln Ile Arg Leu Leu Lys Pro Cys Arg

500 505 510

Val Asn Ala Val Glu Glu Leu His Ser Arg Arg Met Ser Val Arg Ile

515 520 525

Val Lys Asn Trp Val Ser Cys Cys Tyr Val Arg Leu Ser Ala Val Gly

530 535 540

Leu Ser Thr Trp Ser Ala Leu Asp Glu Glu Lys Lys Asn Gly Arg Ile

545 550 555 560

Ser Ser Leu Lys Lys Lys Val Asn Ala Cys Ser Ser Leu Ile Leu Ser

565 570 575

Phe Gln Arg Val Lys Thr Gly Met Glu Ile Leu Leu Trp Ile Leu Leu

580 585 590

Lys Met Glu Ile Gln Ile Phe Lys Ile Cys Leu Gly Glu Met Gln Leu

595 600 605

Cys Gly Gln Ala Phe Gly Ser Phe Ser Ala Asp Phe Ser Ala Pro Gln

610 615 620

Ile Ile Lys Lys Met Lys Lys Arg Ile Met Val His Glu Leu Lys Lys

625 630 635 640

Asn Leu Leu Leu Pro Met Cys Ser Ser Leu Ala Leu Pro Thr Gly Leu

645 650 655

Thr Ser Leu Ile Leu Pro Ser Ser Asp Leu Ala Val Leu Ile Ser Ser

660 665 670

Ser Thr Ser His Phe Leu Met Arg Ser Pro Val Leu Pro Ser Ser Arg

675 680 685

Leu Thr Cys Ala Ser Pro Gln Leu Pro Arg Met Trp Thr Trp Ser Ser

690 695 700

Trp Leu Lys Asn Glu Gly Asn Asn His Trp Pro Arg Val Glu Met Pro

705 710 715 720

Thr Gly Trp Leu Leu Val Gly Tyr Asn Thr Arg Asn Thr Ala Gln Asn

725 730 735

Pro Arg Leu Gln Gln His Leu Lys His Trp Gln Gln Cys Thr Arg Ala

740 745 750

Arg Trp Cys Gln Glu Pro Cys Gln Thr Leu Thr Phe Phe His Phe Phe

755 760 765

Phe Phe Phe Lys Thr Val Ser Leu Cys Cys Pro Gly Trp Ser Lys Gly

770 775 780

Gln Pro Pro Pro Pro Gly Leu Lys Pro Ser Ser Leu Ala Ser Gln Val

785 790 795 800

Ala Arg Thr Thr Gly Val Tyr Gln His Val Trp Leu Ile Cys Phe Val

805 810 815

Phe Phe Val Cys Val Glu Thr Gly Phe Cys His Val Ala Gln Val Gly

820 825 830

Leu Glu Arg Leu Val Ser Leu Ser Gly Ile Thr Asp Val Asn His Trp

835 840 845

Ala Gln Pro Lys Pro Ser Pro Ser Lys Gly Thr Gly Met Asn Arg Pro

850 855 860

Ile Gly Leu Arg Val Gly Lys Gly Val Trp Ala Arg Leu Ile Leu Ala

865 870 875 880

Met Thr Leu Thr Leu Asn Gly Met Ala Lys Lys Ser Gln Gln Pro Leu

885 890 895

Gln Val Val Leu Arg Leu Gly Asn Leu Pro Pro Phe Phe Arg Gly Ser

900 905 910

Glu Val Pro Phe Pro Arg Pro Cys Arg Thr Lys Pro Ala Cys Thr Ser

915 920 925

Lys Ala Trp Thr Pro Arg Lys Leu Val Gly Arg Ala Val Arg Arg Lys

930 935 940

Gly Ser Pro Leu Trp Asp Cys Ser Thr Thr Thr Gly Thr His His Gly

945 950 955 960

Cys Leu Trp Glu Ile Pro Ser Val Thr Leu Leu Ala His Pro Arg Thr

965 970 975

Ala Ser Ile Thr Leu Pro Thr Thr Ser Leu Lys Gly Ser Arg Thr Ser

980 985 990

Cys Cys Pro Arg Ser His Pro Arg Leu Pro Arg Thr Lys Trp Pro Leu

995 1000 1005

Gln Cys Leu Leu His Ser Leu Gly Thr Thr Gly Thr Leu Val Trp

1010 1015 1020

Glu Arg Gly Val Leu Gln Met Arg Ser Leu Glu Ala His Cys Pro

1025 1030 1035

Leu Gln Thr Phe His Leu His His Cys Arg Thr Gln Pro Ser Ser

1040 1045 1050

Cys Pro Pro Ala Trp Ile Leu Tyr Gln Gly Gln Trp Ser Gly Ala

1055 1060 1065

Val Val Gly Leu Arg Pro Val Ala His His Leu Pro Arg Pro Ile

1070 1075 1080

Gln Gly Leu His Cys Pro Gln Ala Pro His Gln Pro Ala Leu Ser

1085 1090 1095

Trp Gly Lys Trp Pro Val Gly Met Thr Gly Pro Ala Arg Cys Cys

1100 1105 1110

Arg Trp Pro Arg Arg Cys Gln Lys Lys Val Pro Ser Ala Ser Pro

1115 1120 1125

Trp Gly Leu Val Leu Lys Lys Asn Arg Lys Gln Met Leu Asn Gln

1130 1135 1140

Lys Cys Leu Ala Pro Phe Arg Glu Ser Thr Leu Gly Asn Phe Trp

1145 1150 1155

Met Lys Leu Leu Gln Leu Pro Val Val Ile Asn Leu Lys Arg Leu

1160 1165 1170

Gln Lys Phe Asn Leu His Leu Cys Tyr Tyr His Ser Gln Ser Asn

1175 1180 1185

Arg Asn Lys Ser Arg Gln Met Glu Ser Leu Gly Met Lys Leu Gln

1190 1195 1200

Ser Gln Lys Ser Gln His His Cys Ala Leu Arg Lys Ile Glu Trp

1205 1210 1215

Ala Thr Lys Leu Ser Leu Gly Gly Lys Ala Gly Pro Gln Ala Gln

1220 1225 1230

Glu Lys Ala Thr Ser Arg Ile Leu Asn Leu Gln Val Leu Lys Lys

1235 1240 1245

Ile Leu Arg Ser Phe Met Lys Leu Tyr His Ser Leu Val Met Cys

1250 1255 1260

Leu Arg Leu Arg Thr Lys Leu Glu Lys Ala Leu Ser Ala Leu Phe

1265 1270 1275

Ile Trp Pro Gln His Ser Tyr Lys Pro Leu Phe Phe His Gly Asp

1280 1285 1290

Leu Cys Glu Trp Gly Gly Gln Ala His Arg Gln Arg Asn Trp Leu

1295 1300 1305

Lys Asn Trp Lys Arg Phe Lys Leu Lys Lys Lys Tyr Ile Gln Lys

1310 1315 1320

Lys Lys Gln Lys Ser Asn Lys Ile Ile Cys Lys Gly His Arg Ile

1325 1330 1335

Thr Ile Ile Cys Ile Lys Asn Met Phe Leu Gln Arg Lys Gly Phe

1340 1345 1350

Leu Asn Leu Ser His Val Glu Lys Phe Cys Arg Gly Gly Pro Ser

1355 1360 1365

Ala Ser Asp Arg Cys Cys Ser Cys Ser Pro Ser Gly Phe Ser Ala

1370 1375 1380

Gly Arg Gly Arg Cys Pro Val Gln Gly Cys Leu Arg Pro His Arg

1385 1390 1395

Val Gln Leu Leu Arg Arg Trp Gly Pro Gly Ser Pro Ala Gly Gln

1400 1405 1410

Arg Leu Ser Lys Gly Phe Gln Leu Leu Arg Trp Trp Gly Pro Gly

1415 1420 1425

Ser Pro Ala Pro Glu Pro Arg Lys Gly Pro Phe Pro Pro Pro Asp

1430 1435 1440

Pro Pro Trp Pro Val Thr Ala Val Thr Val Met Ala Gly Ser Val

1445 1450 1455

Pro Ser Ala Gln Ser Val Asp Ala Leu Glu Ser Pro Gly Pro Leu

1460 1465 1470

Ala Leu Glu Gly Pro Ser Ser Pro Arg Asn Leu Leu Trp Arg Glu

1475 1480 1485

Met Ser Ile Phe Leu Pro Gly Ile Phe Thr Ala Lys Lys Ser Pro

1490 1495 1500

Thr Ser Phe Cys Cys Asn Ile Leu Asn Ser Leu Pro Ser Ser Met

1505 1510 1515

Glu Ile Ala Val Leu Gln Glu Asp Ser Lys Lys Met Thr Gln Lys

1520 1525 1530

Gly Gln Ala Phe Gly Ser Leu Cys Gln Leu Val Ser Ala Asp Phe

1535 1540 1545

Ser Ala Pro Gln Val Thr Arg Leu Arg Ser Leu Asn His Leu Leu

1550 1555 1560

Ile Thr Tyr Thr Pro His Ile Cys Arg His Thr Leu Leu Thr Pro

1565 1570 1575

Asp Thr Arg Trp Ile Phe Ser Asn Gln Leu Gln Thr Val Gln Met

1580 1585 1590

Leu Cys Leu Pro Pro Leu Lys Gln Gly Glu Arg Ile Ile Trp Pro

1595 1600 1605

Leu Gly Gly Phe Gln Ile Pro Asn Phe Phe Trp Ser Leu Gly Ile

1610 1615 1620

Gly His Thr Gly Ala Trp Trp His Thr Gly Arg Arg Arg Arg Glu

1625 1630 1635

Trp Gly Gly Ser Thr Val Ser Arg Ser Pro Leu Trp Ile Ser Ser

1640 1645 1650

Met Ile Tyr Leu Arg Gly Met Ala Phe Ala Ser Asp Ser Ser Ile

1655 1660 1665

Arg Thr Thr Arg Val Ser Trp Cys Arg Arg Cys Gly Ala Lys Ser

1670 1675 1680

Ala Ala Ala Ser Ser Ile Glu Ala Leu Thr Ala Leu Phe Thr Cys

1685 1690 1695

Arg Thr Ala Ser Ile Ser Val Asn Ile Ser Thr Leu Met Ser Ile

1700 1705 1710

Leu Glu Ala Leu Lys Lys Lys Pro Arg Asp Ile Cys Arg Ile Ser

1715 1720 1725

Cys Arg Cys Arg Arg Glu Tyr Arg Val Thr Met Gly Tyr Pro Tyr

1730 1735 1740

Asp Val Pro Asp Tyr Ala Ser

1745 1750

<210> 1187

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 1C poly-FSP

<400> 1187

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaagc accagacggg aaatgccttc tcaggccaca 120

ccttctccac ctggaccttg gagagccgct ctgggcaaag aagtgaagcg gagccagagc 180

aagctgcggc tgagaaacac aagccccacc gagatccaat tgaccatgaa cgacagcaag 240

cacaagctgg aaagccccgc tctgaaacag gtgtcccctg cctctccacc tacacagcag 300

cctcagacac cccaggatag cagacaggtg ctggccgctt cttctcctgg acatcctctt 360

ggccctggcg actgccctag aacaatggct ggaccttctg cctctctgca cccagcatct 420

ggcagagcca gcagaaagac ctggcagaga aggctgagag cctgtagagc tggtggacct 480

gctggatggg ctagacaggc catgacactg aaggccctgg tcaagacctg gacacagttc 540

agaaactacc cgagtcgg agccggaaca ggcggaggac ctgcttgtgt tcctgtggcc 600

gaagcttcta ctggcgcctg gacagctgaa ggcgctgccg gaacaagatg cagagcttct 660

agagcccctt gcagacagct gcctgccagc tctcctacca agtgcgagat ctgcgtgaag 720

agaaggcggc ggagaaagcg gagcagccag tctagatctc ccgtgcctaa gaaacgcgag 780

cggatgatgc tgcctgtgcg ggccagaaca gctagatctg ctctgccaca aggcagagcc 840

cctctggaag gaatgcttct gcctagagca ctgcccagcc tgctgagcag aatgccagcc 900

agagagaga gccggttttg gctggcactg caggctctgg gactcagacc tcctcatctg 960

cctagctgtc accctctgcc tcaactgctt ggacctcctc cacctcatca ggcccctttc 1020

gacaagaaag gcttcgtgat cctgagcaag ggcaagaagc gccggaaaga aatccggcgg 1080

atgaccagac ggaccatgac caccaccaaa atgatgatga tgcggcggac caagcggcgg 1140

aggcgaaaac gtagaagaat gaccatgatg acccgcagaa ccctgctgac caagaaaacc 1200

tgtctgaccc ctagaaccag cgccgccaca aaagctctgc ccagacagac aatcagccag 1260

cggaggccta tccagacacc tcctggaaca agcctggcca tctggggcag actgcctctc 1320

agaccacaga gaggacctcg gcctctgcat agatggctgc agcagacaac aggctgctcc 1380

cagagaagaa tcgccaagaa gcggatcaag ctgaggcggga acacactggc catccggttt 1440

atctctgccc ctcagcctag cagcagcacc gttacagctg ctcctagcgg ccagtacttc 1500

agcagcttct tctacaccag cgaccggacc aagtgtgaaa tctgtgtcaa gaacaccgcc 1560

atcctggcta tcaccctgat cacccctcag gaccctcacc tggaccctgt gtgtcaccca 1620

catagaaggc ctggctggaa gggccaccac agagtggtgt ttagccacca tccaagcggc 1680

tccaccaaga gccattggca gcagagagaa agaggcggag gccgcagaga aaaaaagggc 1740

tgcagagaga tgcccagcag ctggtctagc tctggcagaa ccgccgacaa aaagaacgtg 1800

cggagcagaa cggactgac cctgacaggc acaagaagca gcggcacaag agcctctcca 1860

agcgccgtgt gtctgtgctg ttggacatgc ccctccagaa gcttccctgc cgcatctcca 1920

tctggactga gagcacctgt tggagcccag gctcctcctg aagtgacact gagagccagc 1980

tgtgcctcta gcgcctctaa agcctgtgct ggactttggc cttggggagc ccaagtgcgg 2040

aacaaagtgc ggcagaactt caagcggaac ctggccaaaa agaccctgac taacgccctg 2100

cggagtatgc ccaagagcag aaaccaggtg ctgttcagac ggaatctgac cccaagccag 2160

ctgagaactc tggcccctcc ttacatgctg ctgccccagt ctctgtctca gagcctgtcc 2220

agaaagcaga tccccagcca atcaatgctc gtgaccgcct tcttcctgct cgacatcatc 2280

ctgagaagcc tgctgtactc ccggatgtgc ctggtgcaga tccctacaca cacctgggag 2340

cccgactact tcgtgtccct gcctatgatg ttcagctggg ccactcagag ctttccagtg 2400

ggagccgtgt tcctgaccag acctgccatt ccttgctgca gctgtaccag ccacagcaac 2460

ccttggagcc agtccttcaa gaagaagaag cccctgacat tcagccccag cgtgccaatg 2520

tctctggcct gtagcatcac cgtgtccttc gtgggccaga tcagcacact ggtgctggac 2580

gtgtcctcca tctctcactg gcgcaagcgc aagagagaga agcggtccca gaagtcccgg 2640

cagaaccatc tgcagctgaa gtcctgcaga cggaagatca gcaactggtc caagctgaga 2700

tccccgtgga tcttctaccc tcggctgaaa accttcggcg tgccactggg ctctatcctg 2760

tgtctggcag gctccctgag cacgatgatg atcattagca ccaccagaca agagtgtggc 2820

agacggggcct ccgtgccttg tagacctatg atcggcagcg ctagacccgg accttggcgg 2880

acatctgcta tgccatctgc catgggagtc gccctgccta ccagctgtga aagcggaaga 2940

tctcctcctg ccaccggcgg aagaatgcct ggatctcagt ctatcatgat gagctggatg 3000

cctccactgg ctccctgtgc cgcttgtcct tgttctcctg ctcctacact gtgccctgct 3060

catcctgcaa gagcccctac agccgtgcct gcctttacac ctctgtctgc tcaccctgtg 3120

ccagtgctgt ctggatgtca cctggccgtg cgcacctcta tgctgacact gctgcccatg 3180

agacaccctc acagaagccc acctacaagc tgccctccac tgctggaagc tttcccactg 3240

ccaagggcca gcctgagagc taccgtgcct agacacagct ctgtgctggc cactggactg 3300

catctgcatc ggcagattag cctgcctctg aggggcttcc tgctgcatca acctctgcct 3360

ggacaacacc tgtggctgtc cccagactg aggctgagac tgccaagaac aggcgagaag 3420

aacgacctcc agctgttcgt gatgagcgac accacataca agatctactg gacagtgatc 3480

ctgctgaacc cctgcggcaa cctgcacctg aaaaccacat ctctgcggag agccagacct 3540

acctcctgca gcacatgcag aatgtggtgg ctgaagctgg ccccactgca agtgaccaca 3600

acactgaagc tgatcctggt catgctggaa atcgtgaccg aagaggccaa gaaatccatg 3660

aagagatccc ggcggagcaa gatcgaggcc tctcctatcg gctccatcat gaagaagaaa 3720

gacagcacca tcttcatcaa ccagcagctc aagtggaccc ctcacatcct gaagtctgcc 3780

agcgtgtcca agaagagact gcccaagctg aacggcatct gggaagtgat gccccggaca 3840

ctggaaggct gcatcagcgt gaaggaccac cggacaagcc agaaccacag caagtgcatc 3900

accgagtgtg tgcggacagc cctgaaccag agaaaggtgg tcaagaaccc cagccagcca 3960

gtgccactga gaaacgacgg catcaccaac ggcaacgact ccaccagatc ctgctccatc 4020

agcctgaaga aaagcctgga aaagatgccc ctgatgaacg ccaagaaaat ggaagatatg 4080

atctgggaga gcaacatggg cggcaaccgg tccatctgcc tgacaagact gccctgcaaa 4140

gtgaccgagt ccagccaaag accacctcgg agcattctgt ccctggcctc tagagccgga 4200

cagtttcccg tttggcggag aaccagcaga ggcatgtccc tgcagtggaa gtccggcaat 4260

gcctgcacac ctctgaggtg ccctagcaga agccctgcca catttctgac cgcactgaac 4320

ggctggcaca gatctggact tagggctcag caacctagcc tgccaggacc ttgtgctagc 4380

ctgctgtcta cactgagcca gcctccacca caggctccaa gccaagtttg gacagccgcc 4440

acactgagat gtcccgctgt tcctgcagct gcttgccctc ctatcgtgtt ctttctggcc 4500

ccatacttcc ccacaccact gagcatcagc agacccacct ctcgggccag acagtcttgt 4560

tgtctccccg gactgacaca ccctgctcac cagcctcttg gctctatggg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1188

<211> 4623

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 2C poly-FSP

<400> 1188

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgacgg cagatcaaca gacggaagct gcagagaaag 120

aagcgccgga agaagagaga ggaccactgc ggactgcggg ccagaaaccg gaacatgaag 180

aaaaccatga gccacctgat cgcccatcag gccctgaaga tgtctggcct gagactgttt 240

caccccagac tgctgacacg gaagaagcct ggcaagaacc ctcggagaaa gacctggaag 300

atgcggaaga agtacgccca gaagcctagc cagaagaacc acctgtacgg caagggcctg 360

acacaccctg tgcctctgaa gagaaccctg cagaacaagc ggaacaagaa catcaagcgc 420

gtgaaccaga agaagggcaa catcaagaga aagagcatcc tgctgcacct catcgtgctg 480

aacagccccg agagcaacct gcaggctcat cctcaagttc cagcactggc tcctcaggct 540

tggtggcctg ctagaagagg ccctctgaca agcgctccat ctgctcagca gagcctgacc 600

ttcctgccta gcagatttcc ttggggccct cctccactgc actggaactc tgcctacctg 660

ggcagaacac tgctcgtgac ctgcatcctg taccacagat ggatcgtgac cagcatgtgc 720

cagagccaga gatggctgac cagcaccacc tctagaagca gcgccctgat gtggacccag 780

tggtctagcg tgacaagcct ggaatggaca gcccaagaga ggggctgtag cagctggctg 840

atgaagcaga cctggatgaa gtcttggagc ctgcgggacc ccagctacag atccatcctg 900

gaatacgtgt ccaccagagt gctgtggatg cccacatcta cagtgggagc cctgagagcc 960

acagctcctg caacagctgc tccacaggct cagacccctc attggagagc cgccacaaca 1020

agcagcatga gcagcagccc tagagccgag cctacaagct ctagctcttg ggccacctgt 1080

tggcccagat gttgcagctc cagctgttac tggtccctgt cctcttggcc tcctgctggt 1140

gctgccgagg ccacaaatac caggaccaga agcagagagt cccagcgggg cagaatgctg 1200

acttggagaa gcagcctgtg gctgcagggc accagatgtt ttacaggcgt gcggacctcc 1260

agcagacacc cttcttggcc ttggaccaga tgcctgagaa tgcggcctcc tagaagcaac 1320

agccgggaaaa agctgctcga ggacatgaag caaaatggct tcacccggta caagaaacgg 1380

ggcgtgatag cctggcggag agccactttc ctgctggccc tttgggagtg cagtctgccc 1440

caagctagac tgtgcctgat cgtgtctagg accctgctgc tggtccagag cagatgcagc 1500

ctgatggaac agatccctca cctgaagtcc tggtggtgta caagaggcgg cggaaagcgg 1560

1620

aagggcatct gctggaagtg gcctgtgaca gtggccgctc tgatcctgat tcagcacggc 1680

ggcatcttca ccttccggtt ctgggtcgag aagctgtcct ggaacatctg caagaatctg 1740

tccctgtacc tggacaagtt cgtgcagcgg ctgctgtacg tgtacatcat cgagacaaac 1800

gtgttcgtcc tgaacctgtt ccacgtgcca gtgaatcaga tcttcatgat ggccttctac 1860

ctgaagtaca ccgagaaggc ctgggtcatg gaactggaat ggctcattca gcagcagtgc 1920

taccagttgc tgctggctca tatgtacggc ttcagcacct acggcaccca gaaaggcgag 1980

tgcgtgtgtc tgcagtgtct gagcggcagc tccctgttca tcctgaagca gccactggac 2040

ctgaagatca gcaagacctt catgagcaga tcccacctgg atttcagccc ctattggatg 2100

aagaaagtga aggcctctcc acagatcatg cagcggatga gcgccgtgaa cttcgagaca 2160

atcaggcgga agtggcagat ccggcacgtg ctgaagaacc tgcctctgga aaacacacag 2220

atccccagga caagccctct gtggaagatc acaatggtgc tgacataccc caactgcgtg 2280

gtgcatgtgc tgatgttcgt gctgttcaag gccgaccaga gcgagtctag cctgagcagc 2340

ggaatctacc ctcctagcac ctgtagcctg ccttgccggg gcattagaat gtgcgccctg 2400

tctcacccta gcttctacat cagcagcacc gtgtgctttc tgagcaagca ctgcatgacc 2460

gcctgcacca gagagaagat catcaacccc accatcagct gcccctttca gagccccaca 2520

aagagactgg gccacagaga gggcagcgag agcgataatc tgggcctgct gtactccttt 2580

cctccttggc tgcccatgag atccctgggc gaaccttgga gagctggctt tcagtgggga 2640

gagtcccctt ggcaagtgat ggctagcctg accggcaaca gcgtgatcgt gcacaagatc 2700

gtgctggtgt ttctgaagtc tctgagcagc tacaactgcg agcggcggat ctctggcatc 2760

cagaagagag ccatcgagtt cgccgtcctg aagctgctga acatggacct ggctagccct 2820

cagattatgc agagaaccag cgccgaccgg ttccggatga agcggagaat gcagaacctg 2880

gataacctgg gcgtgccctg gccattgctt ccaggtgctg ctcctccaca tagactgctg 2940

ggcccctcta gatggattct gtttcatgtg cggaacatcg tgtacggcat ccaagtgatg 3000

ctgtgccggc ggagaaacag cagaaagcag agtcaggtgc agctggccat tcaggtcctg 3060

ctcgtggaca agcacaagta ctttctgttc ggcccctatc ctatcagcga gggactgacc 3120

atgaccaccg gccggaacat caacagcaac tccctgatga ccaactgcct gttcagcccc 3180

atcttcctgt gccaccacgc catcatgcac cggaagatga caagcccaca cttcagactg 3240

ttctccagca agatcccaca tcctcaggtg ccaagtgtgg tggccctgtg caagttcctg 3300

gccaatggcc tgtggttcaa cctgatcttt cagaaaaagg gccacatgat ctttagcccc 3360

3420

cacacccagt gccggaacaa aaagaccaag ctgtggtttt ccctgatcaa catccaccac 3480

3540

tctccacctc tgcatctgtg ccaacctctg agaggcggca agaagagaag caagttcgcc 3600

ttctggaagc ctcctagcca gggcatcagc tcttgtctga tgacaggctg ttggggctcc 3660

3720

3780

acccagcgga agcggatgaa gaccggcgcc attcaagaga agggcgctat ccaagaaaaa 3840

ggcgcaatga ccaagcggat ctgcgtggtc aatttcgagg cctgcagaga gatggaaagc 3900

ctgagcgccc ctgagaagat taccctgttc gaagcccaca tggcccggtg cacctccatc 3960

aatgtgctgt gtgtccgggc cagcctgatt gagaagctga tgaaagaaaa gcgcaagatg 4020

aagcgcaacc aggtggcatc ccctcaaatc atgcaacggc tgttcatcaa gggctaccag 4080

aacaaccact tcacgttcca cctccaaaac tttgccttct tcagctcctt cggcaacaac 4140

aagtatatca acattctcac ccgggccttc atccacacac ctagaagccc tggctgttcc 4200

gtgtacgcca atatgtgctg gcggaagatg cagtccctga atcaccccag cagactgacc 4260

aactgtcacc ccgcttggct gagccccatt agtgcttgtc ctaggctgct gccctatcct 4320

tttcacgtgc tggcactgga agtgaccttt ccacctggca cagccatgcc tcagatgcaa 4380

ttgcaggcac tggctatgtg ggagaaaggc tgggacaagc aagagaagaa atcccgcaag 4440

ctgatgtcct cctgcctgga aaaaaaggct acctggatgc ggagaacccc tggcacaagc 4500

ctgcagtgtt acctgaacac acacccctgc accatgctgc tgcctcaggg aatcccatct 4560

ctgaacgccc agtggatgtt cctgcggatc ggctacccct acgatgtccc tgactatgcg 4620

tcc 4623

<210> 1189

<211> 4638

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 3C poly-FSP

<400> 1189

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgacct attcctccac acctgagaca ccctctgctg 120

ctgaagaaca agaagcggag aaagctgcgg cccaaggca gaaagaaggg ccacaccaag 180

aaccggcgga aggccaagag aaagccccag atcagaaaca gccgcagagc cacacctacc 240

agcaagggca gcctgagaag aaagtacctg agagtgggag ccgccgctgc tagctgtcaa 300

tctcctccat ctcctagaga gacaacccct agcagcgacg ccaccagaag aaccagacct 360

atcgccgctc agagcaagaa aaccatgaca gccgccggac tgacccctag ccagaagaaa 420

gatcctgccg gcaagctgaa gaaacaggtg tccgtgtaca gcggcaacct gctgagcttc 480

tctcggagag gcatgaagtc tagcgtgcgc ctgaagaact gcaccaacct gagcgtgccc 540

atgatgctga ccatcctgat ctggaagcgg gtgttcatcc tgctgctgtc cgacaagaaa 600

tctatccctc acgtgcccaa ggccaccgcc gctcattctg tgacagtgaa gctgcagagc 660

cggcggaaca gacggtacag aaagatctat ggcgagctga agatgtccct gcaggccagc 720

aatggcatgg aagcctgtgg cacacacccc acacctagac tgtccgtgtc cgtgatcacc 780

atgaagctgt gggagaagca cttcaagggc ctgaactgga acctggtcgt gtggattctg 840

aacctgaaga tgatgctcaa gaagaagtat ctggtccagc gcgagtggga gaaacggaag 900

cagattcttg gcggcgggagg caagtacaaa gagtacttcc tgaagagaat cctcatccgc 960

aaggccatga cagtgctggc cggcgataag aaaggcctgg gcagattcat gagatgcgtg 1020

cagagcgaga caaaggccgt cagtctgcag ctgcctctgg gcagaatgga cctggatgga 1080

atgctgaccg tgatcggcca gggactcgtg ggcatgacac ctagagatct gggaacagtg 1140

gctggcgcca ccgcacagat tcagaggcct attcaggtgc agacaggcgg cagactgaga 1200

ggccctagaa gaaaggtgct gatggtgctg tggctgcccg ccaatcagag cctgcagaaa 1260

agccaggtgt tccagtgggt gctgagaacc gagctgcccc tgacctttag caccctgctg 1320

tgggacagca gacacgacct gcctagaatg ctgcccatca gcagccctct gcacagaccc 1380

cagatgcctt tctggacctg cacctttcct gccaccacat ggggcacatg ggagacacct 1440

tctacatggg gctggcggac ctttgccaga cagccttcta gacccaccgg cagaacccct 1500

accaccagac tgatggctcc agtgggcagc gtgatgagct gtagcctgct gacacagcca 1560

tctccagcca gcgcctgtac aatgcctcca ctgctgcatc ctcagtgggc ccctcattct 1620

gctggacttt ctccaggcgc ttgcagacct gcctgtacct gcatcagcat gaccacaatc 1680

agcagggcca cctggcgcag aaacctgcag agaaacctga aagtgctgcg gctgagaatc 1740

cccagcctgc ggaagtacag acagcagaac acccggtcca ccaacctcga gaagaaccca 1800

tctctgaaga agcagagcca gaggcggggc aagctgtctc tgaccacact gagcttcgtg 1860

ctgatcgagg aactgctgaa aaagagcagc cagctgcgga gaagctggcg gaacgtgtcc 1920

agaatcagca acatccggaa gctgatttgg accaagcagc ggagcttcct gtctctgttc 1980

cccaagctga gagccaccga gcagatgacc aatgtgggct gtagactgga ttttcacccc 2040

agaagcagct ccggcgacag agtgttcctg gtcaagatcc agagcagccc attcctggcc 2100

agatcctgga ataccatgca gtggaacaac aagaaaatgg ccatgactgt gatcgctggc 2160

gcccaccact gtcagtacaa gctgacctac accaaagtgt ggaagatgaa ttggaagaaa 2220

atgatgatcg gcagccagca cctcctgttc tccccactgg atagctctcc actccagctg 2280

ctgctgcacc cacacggcaa gagatgcaac ccctgttgtc ggctgacatg gatgagccac 2340

ctgaatcgga agctgcacct caccaggtcc tgcctgatcg acctgaccat gatgatgagc 2400

cagaaactgt ggaagaatca gcggaagcgc atccctctgc cttctccaag acagcatctg 2460

ctgcctcagt atcctctgca ccctcctcca ccttgtctgc tgcttctcca ccatagacac 2520

cacctggcat tcctcgagat ggcctgtagc tctcagcaca cccacagcat caagatctgg 2580

atcagcttca gccacgagac aatgagcctg tccaacatcc tgatgaagtt ccggctgacc 2640

accaccacaa gcagccccag aagaagccct gctctgcgag ctagaggcgg cacaacaatc 2700

ggcgaagtga ccagcagcct gttctttagc cactgcctgt gcgtggcccc tagatgtcct 2760

agatctgccg tggacatcag ccccggacct acatctcctt ctctggcctc ttgtgcctct 2820

gtggccattg gaaagctggc actgcttggc gcctctgctc cttgtggtgc tgctggcgct 2880

agagaggccg gaagatttgc tgtgctgggc accatggtca tgaagaacat ggaacacaga 2940

ctgaccatgg acagcgccct gagcctgaca aagagagagg gccctgaaac aggccccgtg 3000

3060 tactacgtgt gcaaccggat gaatgtgtcc

3120

ctgaaaccct gtctgatccc cgtgccaatc tgcaagaaga aactgctgct caagctgctg 3180

aggggcgcct ccagctctac cagcagagat agcagaagct ttttcaccag cgctcacccc 3240

agcagaccct gcaggatgag actgtaccag atgctgcaga agcagtgctg ctacaacctc 3300

gtgatcaagc accctctgat cttccggatc aacctgcgga gacaggggcaa gaccaaagac 3360

ggcgttgtgc agagcctggg cttcctgaac cagcagatca tcctccagaa agagttcttc 3420

atctgcctgc tgatgttcat caagcggtgg aagccagccg ccaccaagaa gggcaagaac 3480

agcccctgct gccagaaaaa actgcgggtg cagaaccagg gccatctgct gatgattctg 3540

ctgcacaaca gaggcccacc tctgcatacc agattcgaga aaaagcggcc cgagtggctg 3600

agcagaaaat ccggcgtgat gaagtgctgg gatctgtgcc ctgccttcag cggcaggatt 3660

ctgcaccagc cagaaatggc cgccatgaga ctgatgccca agttcctgaa ctccaccaac 3720

agctggtgga cagtgcacaa gaagagaggc ctgttccacc agatgaagtg tctgaccgcc 3780

gtggactgtc tgcaggcctt ctgggatatg agcgagaaga aacgcaagct gcctatgatt 3840

cgcaaaatcc agaagcggcg gagaaccgcc aaccggaaca tcgagatcac ccctcagaga 3900

agcaaagccc acctgaagaa aacctccgcc aagggcgcca agaaaatcac cttcctgtgc 3960

ctgagagtca gatgcggagg ctggctgtgt ggctggacac caagaggatg gctgaagaag 4020

tccaccaagt ccagactgtg cttcttctac ctgctgtact acctgttttt cctgaccgtg 4080

tgcggcttca cccctccacc acttattctg tggcctcagg cagcccctgg cgagaagctg 4140

aagcactgtt ttaaagcctg gggcgtcgac cctcctggca gcgtttggat tctgccctgc 4200

ttcctgattc acctgagaaa ggtggacacc caccacctcc aggtggtcac caaaacagtg 4260

accgccacaa cagccgacgg ccagaggatg agaaggctga agtgcaaggc ctgccggctg 4320

agatgggcta gaccctgaacc tgcagctcag gcccctccta agagccactt tccacctcat 4380

cctagcggcc cctccagaac cagctctctg caattgagaa cctacatgga aatgcactac 4440

ggccatcagg ccccacaggt ggcaatgctg cctaaactgc tgtgtcggca gatgctcccc 4500

actctgtcta ccctgcagca gagacatcct cagggcccat gtcctcctcc agtgacacct 4560

ctgagagtgc agtctgtgct gctgctcgga gtgcctcaaa cagctggcta cccctacgat 4620

gtccctgact atgcgtcc 4638

<210> 1190

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 4C poly-FSP

<400> 1190

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgccgagcc aggaaatcca tgcccgagac agcaagaaga tgacccagaa gaaccgcaag 120

acccggaagg ccgccagaga gaagcacaga aagaccgaca acctggacct gcagcaccag 180

cgcagacgca aaaagaagaa ggacaaggtc gagatccgga atcggagccc taaggccaat 240

agcaccgagt gtcagggcga gcacagcaga gagcagaacc tgctgaacct ggtgcaattg 300

aaccctcaca gccctcacca cgggaaggac gaggaagatc accagaagag acaccaccac 360

cacaatcaaa agaagatgtc cgtcaacgag ggcaacaacc actggcctag agtggaaatg 420

cctaccggct ggctgctcgt gggctacaac accagaaaca cagcccagaa tcctcggctg 480

cagcagcacc tgaaacactg gcagcagtgc accagagcca gatggtgtca agagccctgt 540

cagaccctga ccttcttcca cttcttcttc ttttcaaaa cggtgtccct gtgctgcccc 600

ggctggtcta aaggacaacc tcctccacct ggcctgaagc cttctagcct ggcttctcag 660

gtggccagaa ccacaggcgt gtaccagcac gtgtggctga tctgcttcgt gtttttcgtg 720

tgcgtggaaa ccggcttctg tcacgtggcc caagtgggac tcgagagact cgttagcctg 780

agcggcatca ccgacgtgaa ccattgggcc cagcctaagc catctcctag caaaggcacc 840

ggcatgaaca gacccatcgg cctgagagtt ggcaaaggcg tgtgggctag actgtggctg 900

aagaactgga agcggttcaa gctcaaaaag aaatacacag gcatggaaat cctgctgtgg 960

1020

ctcaagaaga aagtcaacgc ctgcagcaac gccaagaaaa tgcccttcca gtgccggtgc 1080

agacgcgagt acagagtgac catgattcag aagaagaaac agaagtcccc gctgttcttc 1140

cacggcgacc tgtgcagacc ttctccttgc ctgtcttgga gcagcaggat cgtggctcct 1200

ggacacgccc tgagctgtcg gactcgtaga agatggtcca gcaagatggc caagaagtcc 1260

cagcagcctc tgtgtagagg cggcccttct gccagcgata gatgctgtag ctgtagcccc 1320

agcggcttta gcgctggaag aggcagatgt cctgtgcagg gatgtctgag gccacacaga 1380

gttcagctgc tgagaagatg gggccctgga tctcctgctg gccagagact gtctaagggc 1440

ttccaactgc tgcgttggtg gggaccagga tctccagctc ctgagcctag aaagggccca 1500

tttccaccac ctgatcctcc atggcctgtg accgctgtga cagtgatggc tggctctgtg 1560

cctagcgctc agtctgtgga tgctctggaa tcccctggac ctctggctct cgaaggccct 1620

agcagcccta gaaacctgct ctggcgggaa atgagcatct tcctgcctgg catcttcaac 1680

ctgcacctgt gctactacca cagccagagc aaccggaaca agagccggca gatggaatcc 1740

ctgggcatga agctgcagat cacacaccca ttcgccagcc agccactgggg ccaagagatg 1800

cccaacaaca cccagatcag actgctgaag cccggccagg cctttggcag cttttccgcc 1860

gatttttcag cccctcagca ggcccctctg agactgtggt cttggtgcgg cacaagccag 1920

acctacttcg gctctatcga agccctgaca gccctgttca cctgtagaac cgccagcatc 1980

agcgtgaaca tcagcaccct gatgagcatc ctggaagcca tcctggccat gacactgacc 2040

ctgaatggca gccctctgtg ggactgcagc acaacaacag gcacacacca cggctgcctg 2100

tgggagatcc cttctgttac cctgctggct accgccaaaa agagccctac cagcttctgc 2160

tgtcagcggc ctcctctgca gccagtggaa gagaagaaga acggccggat cagcagcgtg 2220

ccaccttctc cacctcttgc tctgggccct agaatgcagc tctgcaccca gctggcccgg 2280

ttcttcccta ttacacctcc tgtgtggcac atcctgggac ctcagagaca cacccctctg 2340

ctgacccctg acaccagatg gatcttctcc aaccagctgc agaccgtgca gatgctgtgt 2400

ctgcctccac tgaagcaggg cgagagaatc atctggcctc tcggcggctt tcagatcccc 2460

aacttctttt ggagcctcgg catcggacac actggcgcct ggtggcatac tggaaggcgg 2520

agaagagaat ggggcggcag caccgtgtct agaagcccac tgtggatcag ctccatgatc 2580

tacctgagag gcatggcctt cgcctccgac agcagcatca gaaccaccag agtgtcctgg 2640

tgtcggagat gtggcgccaa aagcgccgca gccagcagcg acaagaagaa gaaaagcacc 2700

gaaaagacca agaacaagat catctgcaag ggccacagaa tcacccaggt ggtgctgaga 2760

ctgggcaacc tgcctccatt cttcagaggc agcgaggtgc cctttcctag accttgccgg 2820

accaagtgcc agaagaaggt tccaagcgcc tctccttggg gactgcaccc aagaacagcc 2880

tccatcacac tgcctaccac cagcctgaag ggcagcagaa caagctgctg ccccagatct 2940

caccccagac tgcccagaac aaagtggcct ctgcagtgcc tgctgcacag cctgggaaca 3000

actggaacac tcgtgtggga aaggggcgtg ctgcagatga gaagtctgga agctcactgc 3060

cctctgcaga cattccacct ccaccactgc agaacccagc ctagctcttg tcctccagcc 3120

tggattctgt accaaggaca gtggagcgga gccgtcgtgg gacttagacc tgtggctcac 3180

catctgccaa gacctatcca ggggctgcac tgtcctcagg ctcctcatca accagctctg 3240

tcctggggaa agtggcccgt gggaatgaca ggacccgcca gatgttgccg gtggcctaga 3300

agaatgtgta gctctctggc cctgccaacc ggcctgacat ctctgatcct gcctagcagt 3360

gatctggccg tgctgatctc tagctccacc agccactttc tgatgcggag cccagtgctg 3420

ccaagcagca gactgacatg tgcctctcca cagctgcctc ggatgtggac atggtctagc 3480

3540

gaggaactgc acagcaggcg gatgtctgtg cggatcgtga agaattgggt gtcctgctgt 3600

tacgtgcggc tgagtgcagt gggactgtct acttggagcg ccctggattc cctgatcctg 3660

tccttccaga gagtgaaaac cagccggata ctgaacctcc aggtgctgaa gaagatcctg 3720

cggagcttta tgaagctgta ccactctctc gtgatgtgcc tgcggctgcg gaccaagctg 3780

gaaaaagctc tgagcgctct gtttatctgg ccccagcaca gctacaaagg ccaggcattc 3840

ggaagcctgt gccagctggt gtctgccgat ttcagcgccc ctcaagtgac cagactgcgg 3900

agcctgaacc atctgctgat tcggtggatc tttagcaatc agctgacagt ccaaatgctc 3960

tgcctgccga tcatcaagaa aatgaagaaa cggatcatgg tgcacgtctt gaagaagaat 4020

cggaagcaga ttatctgtat caagaatatg ttcctgcagc ggaagggatt cctgaacctg 4080

agccacgtgg aaaagttcag agagagcacc ctgggcaatt tctggatgaa gctcctgcag 4140

ctgccaccag cttgcacctc taaggcctgg acacccagaa agcttgtggg gagagccgtg 4200

cggagaaagg gcgtcgtgat caacctgaag agactgcaga aattccggggc tagcgtgtcc 4260

ttctggactc tgctgcctac aacaggcgtg cggacaagag gctctccact gagactgact 4320

ctgtggcctc caagaagcca gaaaagccag caccactgcg ccctgagaaa gatcgagtgg 4380

gccacaaagc tgtccctcgg cggcaaagca ggaccacagg ctcaagagaa ggccaacatc 4440

ctgaacagcc tgccttcctc tatggaaatc gccgtgctgc aagaaatgct gaaccagaag 4500

tgtctggccc ctttcgagct taagaagaac ctcctgctgc caacctacac accccacatc 4560

tgtcggcaca cccttaaaaa gaagcccaga gacatctgca gaatcagcgg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1191

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 1D poly-FSP

<400> 1191

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgagcc gacaagaaaa acgtgcggag cgctagttct 120

cctggacatc ctcttggccc cagaagaatg accagacgga ccatgaccac caccaaaatg 180

atgatgatgc ggcggaccaa gcggcggaga agaaaaagac gccggatgac catgatgacc 240

agaagaaccc tgctgaccaa gaaaacctgt ctgaccccta gaaccagcgc cgccacaaaa 300

gctctgccca gacagacaat cagccagcgg aggcctatcc agacacctcc tggaacaagc 360

ctggccatct ggggcagaag aagcaagatc gaggcctctc ctatcggcag catcacccct 420

ctgagcatca gcagacctac cagcagagcc agacagagct gttgcctgcc tggactgaca 480

caccctgctc atcagcctct gggctctatg gttggagctg gaacaggcgg aggacctgct 540

tgtgtgcctg tggccgaagc ttctacaggc gcttggacag ctgaaggcgc tgccggcaca 600

agatgtagag ccagcagagc cccttgcaga cagctgcctg cttctagccc taccaagtgc 660

gagatctgcg tgaagagaag aaggcggcgc aagcggagca gccagtctag atctcccgtg 720

cctaagaaac gcgagcggat gatgctccct gtgcggggcca gaacagctag atctgctctg 780

ccacaaggca gggctcctct ggaaggaatg cttctgccta gagcactgcc cagcctgctg 840

agcagaatgc ctgccagaag agagagcaga ttctggctgg ccctgcaggc ccttggactc 900

agacctcctc atctgcctag ctgtcaccct ctgcctcaac tgcttggacc tcctccacct 960

catcaggccc ctggcgagaa aaacgacctg cagctgttcg tgatgagcga caccacctac 1020

aagatctact ggaccgtgat cctgctgaac ccctgcggca acctgcacct gaaaaccacc 1080

agcctgaacg ccaagaaaat ggaagatatg atctgggaga gcaaccagca gctgaagtgg 1140

acccctcaca tcctgaagtc cgcctctatc gccaagaagc ggatcaagct gcggcggaac 1200

agcaccagac gggaaatgcc ttctcaggcc acaccttctc cacctggacc ttggagagcc 1260

gctctgggca aagaagtgaa gcggagccag agcaagctga gactgagaaa cacaagcccc 1320

ttcgacaaaa agggctttgt gatcctgagc aacatgggcg gcaaccggtc tatctgcctg 1380

accatcgtgt tctttctggc cccttacttc cccatgccta gcagctggtc tagcagcggc 1440

agaaccagac tgcctctgag gccacagaga ggccctagac ctctgcatag atggctgcag 1500

cagaccaccg gctgctctca gagaagaggc gactgcccaa gaaccatggc cggaccatct 1560

gcttctctgc accctgcatc tggcagagcc tccagaaaga cctggcagag aaggctgaga 1620

gcctgtagag caggcggacc tgctggatgg gctagacagg ccagaaaacg caagagagag 1680

aagcggtccc agaagtccag acagaaccat ctgcagctga aaagctgcag acggaagatc 1740

agcaactggt ctaagctgcg gagcccctgg atcttcgagg ccaaaaagag catgaagaga 1800

tcccagcggc ctcctcggag catcctgagc ctgttcaaga agaagaagcc cctgacattc 1860

agccccagcg tgtccaagaa gaggctgcct aagctgaagg gcaagaaacg cgggaaagaa 1920

atccagcgga aggtggtcaa gaaccccagc cagcctgtgc cactgagaaa tgacggcatc 1980

accaacggca acgacagcac ccggtcttgc agcatcagcc tgaagaaaag cctggaaaag 2040

atgcccctga tgcggagcct gctgtactcc agaatgtgcc tggtgcagat ccccacacac 2100

acctgggagc ccgactactt tgtgtccctg cctatgatgt tcagctgggc cacacagagc 2160

tttccagtgg gcgccgtgtt tctgaccaga cctgccattc cttgctgtag ctgtaccagc 2220

cactccaatc cttggagcca gtccacactg gccatccggt ttatctctgc ccctcagcca 2280

agcagcagca ccgttacagc tgctcctagc ggccagtatt ttgccctgcg gagtatgccc 2340

aagagccgga accaggtgct gtttcggaga aatctgaccc caagccagct gcggacactg 2400

gcccctccat atatgctgct gcctcagtct ctgtcccaga gcctgtctcg gaagcagatc 2460

ccatctcagt ccatgctcgt gcggaccagg ctgacactga caggcactag aagctccggc 2520

accagagcct ctccatctgc cgtgtgtctg tgctgctgga catgccccag cagaagcttt 2580

cctgccgcct ctccaagcgg actgagagca cctgttggag cacaggctcc tccagaagtg 2640

acactgagag ccagctgtgc cagctccgcc agcaaagctt gtgcaggact ttggccttgg 2700

ggagcccaag tgcggatgaa gaagaaggac agcaccatct tcatctaccc caggctgaaa 2760

accttcggcg tgccactgggg cagcattctg tgtctggctg gaagcctgag cacgatgatg 2820

atcattagca ccaccagaca agagtgcggc agacggggcca gcgtgccctg tagacctatg 2880

atcggaagcg ccagacctgg accatggcgg acaagtgcta tgccttctgc catgggagtc 2940

gccctgccta ccagctgtga aagcggaaga tctcctcctg ccaccggcgg aagaatgcca 3000

ggatctcagt ctatcatgat gagctggatg cctccactgg ctccctgcgc tgcttgtcct 3060

tgttctcctg ctcctacact gtgccctgct caccctgcta gagcaccaac agccgtgcct 3120

3180

cggacctcca tgcttacctt gctgccaatg atgaccctga aggccctggt caagacatgg 3240

acccagttca gaaactaccc cagacggcac cctcacagaa gcccaccaac aagctgtcct 3300

ccactgctcg aggcctttcc actgccaaga gccagcctga gagctaccgt gcctagacac 3360

tctagcgtgc tggccacagg actgcatctg cataggcaga ttagcctgcc tctgcggggc 3420

ttcctgctgc atcaaccact gccaggacaa cacctgtggc tgtcccacag actgagactg 3480

cggctgccta gaacaaccga gatccagctg accatgaacg actccaagca caagctggaa 3540

agccccgctc tgaaacaggt gtccccagct agtcctccta cacagcagcc tcagacaccc 3600

caggattcta gacaggtgct ggccgtgcct atgagcctgg cctgtagcat caccgtgtcc 3660

ttcgtgggcc agatctctac cctggtgctg gacgtgtcct ccatcagcca ctggaacggc 3720

atctgggaag tgatgcccag aacactggaa ggctgcatct ccgtgaagga ccaccggaca 3780

tcccagaacc acagcaagtg catcaccgag tgtgtgcgga cagccctgaa caccaagtgt 3840

gaaatttgtg tgaagaacac cgccatcctg gctatcaccc tgatcacccc acaggaccct 3900

3960

gtgtttagcc accatccttc cggcagcacc aagtctcact ggcagcagag agaaagaggc 4020

ggaggccgca gagaaaagaa gggctgtaga

tggcggagaa catctagagg catgtccctg cagtggaagt ccggcaatgc ctgcacacct 4140

ctgagatgtc ccagcagatc tccagccacc ttcctgactg ccctgaacgg ctggcataga 4200

tctggactga gggcccagac cgccttcttt ctgctggaca tcatcctgcg gagagcccgg 4260

cctacaagct gcagcacatg tagaatgtgg tggcggctgc cctgcaaagt gaccgaaagc 4320

tccctggcca agaaaaccct gaccaatcag cctagcttgc ccggaccttg tgctagcctg 4380

ctgtctacac tgagccagcc accacctcag gctcctagcc aagtttggac agccgctaca 4440

ctgcggtgtc ctgctgtgcc tgctgctgca tgtccacctc tgaagctggc tccactgcaa 4500

gtgaccacaa cactgaagct gatcctggtc atgctggaaa tcgtgaccga gaacaaagtt 4560

cggcagaact tcaagcggaa ctccagcttc ttctacacca gcgacagagg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1192

<211> 4623

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended 2D poly-FSP polypeptide

<400> 1192

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgacgg aagatgcaga gcctgaatca ccccagcaga 120

ctgaccaaca atcctcggag aaagacctgg aagatgcgga agaagtacgc ccagaagcct 180

agccagaaga accacctgta ccggcagatc aaccggcgca agctgcagag aaagaagtcc 240

agaagccacc tggacttcag cccctactgg atgaagaaag tgaagcgggc cagaaaccgg 300

aacatgaaga aaaccatgag ccacctgatc gcccatcagg ccctgaagat gtctggcctg 360

agactgtttc accccagact gctgaccaga aagaagcccg gcaagcagaa aaagggccac 420

atgatcttca gccggcggaa gtggcagatc cggcacgtgc tgaagaatct gatccccagc 480

ctgaacgccc agtggatgtt cctgagaatc gctagcccac agatcatgca gagaaccagc 540

gccgaccggt tccggatgaa gcggagaatg cagaacctgg acaatctggg cgtgccctgg 600

cctctgttgc ctggattgct gctggcccat atgtacggct tcagcaccta cggcacccag 660

aaaggcgagt gcgtgtgtct gcagtgtctg agcggcagca gcctgttcat cctgaagcag 720

cccctggacc tgaagatctc caagaccttc atggccagcc ctcagattat gcagcggatg 780

agcgccgtga acttcgagac aatcctgcag gcccatcctc aggtgccagc tcttgctcct 840

caagcttggt ggcccgctag aagaggccct ctgacatctg ctccaagcgc acagcagtcc 900

ctgacacgga cactggaaag cgccggactg tttccttggc tgcacaccca gtgccggggcc 960

ttcattcaca cacctagaag ccctggctgc agcgtgtacg ccaacatgtg ctggcggtac 1020

aagaaacggg gagtgatcgc ctggcggatc taccctccta gcacatgcag cctgccttgc 1080

agaggcctga tcctgattca gcacggcggc atcttcacct tccggttctg ggtcgagaag 1140

ctgagctgga acatctgcaa gaacctgagc ctgtacctgg ataagttcgt gcagcggctg 1200

ctgtacgtgt acatcatcga gacaaacgtg ttcgtgctga acctgttcca cgtgccagtg 1260

aaccagatct tcatgatggc cttctacctg aagtacaccg agaaggcctg ggtcatggaa 1320

ctggaatggc tgatccagca gcagtgctac cagcggaaga gaatgaagac cggcgccatc 1380

caagagaagg gcgctattca agagaaaggg gccatgacca agcggatctg cgtggtcaat 1440

ttcgaggcct gcagagagat ggaaagcctg agcgcccctg agaagatcac cctgtttgaa 1500

gcccacatgg cccggtgcac cagcatcaat gtgctgtgtg tcagggccag cctgatcgag 1560

aaactgatga aagaaaagcg caagatgaag cgcaaccagg tggcatcccc tcaaatcatg 1620

caacggctgt tcatcaaggg ctaccagaac aaccacttca cgttccacct ccaaaacttc 1680

gccttcttca gctccttcgg caacaacaag tatatcaaca tcctgacctt tctgcccagc 1740

agattccctt ggggacctcc tccactggga gcccttagag ctacagctcc tgctacagct 1800

gctccccagg cacagacacc tcattggaga gccgccacca caagcagcat gagcagctct 1860

ccaagagccg agcctaccag ctctagctct tgggccactt gctggcctag atgctgcagc 1920

tccagctgct attggagcct gtctagctgg cctcctgctg gcgctgccga agccacaaat 1980

accagaacca gaagccgcga gagccagcgg ggaagaatgc tgacttggag aagctccctg 2040

tggctgcagg gcaccagatg ttttacaggc gtgcggacca gctccaggtg cagcctgatg 2100

gaacagatcc ctcacctgaa gtctaacagc cggaagaaac tgctcgagga catgaagcag 2160

aacggcttca ccagacggcg gaacagcaga aagcagagcc aagtgcagct ggccatccag 2220

gtgctgctgg tggacaagca caagtacttt ctgttcggca gagccacatt tctgctggct 2280

ctgtgggagt gtagcctgcc tcaggctaga ctgtgcctga tcgtgtctag aaccctgctg 2340

ctcgtgcagt ctagacaccc aagctggcct tggaccagat gcctgagaat gcggcctcca 2400

cgcagccact ggaactctgc ctacctgggg agaacactgc tcgtgacctg catcctgtac 2460

cacagatgga tcgtgaccag catgtgccag agccagagat ggctgacctc caccacctct 2520

agaagctctg ccctgatgtg gacctggtgg tgtacaagag gtggcggcaa gcggaagaaa 2580

aagatgtgca aggtgtccgg cggctgcacc gcctgtgttg aaagatgtcc ttggaagggc 2640

atctgttgga agtggcctgt gacagtggcc gccatgagca gaacacctcc aagacgcgtg 2700

tgcggccaca gagaggggcag cgagtctgat aatctgggcc tgctgtactc ctttccacct 2760

gtgctgttca aggccgacca gagcgaaagc tctctgagca gcggcaacag cgtgatcgtg 2820

cacaagatcg tgctggtgtt cctgaagtcc ctgagcagct acaactgcga gagaagaatc 2880

agcggcatcc agaagagc catcgagttc gccgtcctga agctgctgaa catggacctc 2940

ggcaagggac tgacacaccc cgtgcctctg aagaggaccc tgcagaacaa gcggaacaag 3000

aacatcaagc gggtcaacca gaagaagggc aacatcaaga ggaagtccat cctgctgcat 3060

ctgattgtgc tgaacagccc cgagagcaac cgggaaaaga tcatcaaccc caccatcagc 3120

tgcccctttc agagccccac aaagagactg ggaagccctc ctctgcacct gtgtcagcct 3180

ctgaggccct atcctatcag cgaggggcctg accatgacca ccggcagaaa catcaacagc 3240

aactccctga tgaccaactg cctgttcagc cccatcttcc tgtgccacca cgccatcatg 3300

caccggaaga tgacaagccc tcacttcaga ctgttctcca gcaagatccc acatccacag 3360

gtcccaagtg tggtggccct gtgcaagttt acacacccct gcaccatgct gctgccccaa 3420

ggcgcttttt ggaagcctcc tagccaggggc atctccagct gtctgatgac aggctgttgg 3480

3540

ctgatgaagc aaacatggat gaagtcttgg tccctgcggg accccagcta cagatccatc 3600

3660

ctgaccttca gccagatccg gatgtgcgcc ctgtctcacc ctagcttcta catcagctcc 3720

accgtgtgct ttctgagcaa gcactgcatg accgcctgca cacctggaac cagcctgcag 3780

tgttacctga acctggccaa cggcctgtgg ttcaacctga tcttccggaa caaaaagacc 3840

aagctgtggt tctccctgat taacatccac caccgcaaga acccactgct gcccatgcgg 3900

ctggaaaaaa aggccacttg gatgcggcgg accgagctgt ctctggaacc ttggattcct 3960

tacctgcatc agcagaaaac ccagtggctc cccatgagaa gcctgggcga gccttggcga 4020

gctggatttc agtggggaga gtctccctgg caagtgatgg cctctctgac aggcagacgc 4080

aagaaaagag aggatcactg cggactgcaa gagaaaaaaa gccggaagct gatgtccagc 4140

tgcgccgctc ctccacatag actgctggga cctagcagat ggggaagctg tgggagagcc 4200

tgttcttgtg gcagaggctg tggaacccct agaggcgact ggaagagaca cccacctcgg 4260

cggattctgt ttcacgtgcg gaacatcgtg tacggaatcc aagtgatgct gtgctgtcac 4320

cccgcctggc tgtctccaat cagcgcttgt cctcgcctgc tgccttatcc attccacgtt 4380

ctggccctgg aagtgacatt ccctccaggc actgccatgc ctcagatgca attgcaggca 4440

ctggcaatgt gggagaaagg ctgggacaag cctctggaaa acacacagat ccccaggaca 4500

agcccactgt ggaagatcac aatggtgctg acatacccca actgcgtggt gcacgtcctg 4560

atgttcggcg gcaagaagcg gagcaagttc ggctacccct acgatgtccc tgactatgcg 4620

tcc 4623

<210> 1193

<211> 4638

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 3D poly-FSP

<400> 1193

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgacag aacaccagaa gcaccaacct ggaaaagaac 120

cccagcctga agaagcagag ccagcggaga ggaaagctga gcagcagaaa gaagggccac 180

accaagaacc ggcgggaaggc caagagaaag ccccagatca gccagaagaa ggaccctgcc 240

ggcaagctga agaaacacct gaaccggaag ctgcacctga ccagatcctg cctgatcgac 300

ctgaccatga tgatgagcca gaagctgtgg aagaaccagc gcaagagaat ccctctgcct 360

tctccacggc agcatctgct gcctcagtat ccactgcatc ctccacctcc atgcctgctg 420

ctgctccacc acagacacca tctggccttt ctcgagatgg cctgcagcag ccagcacacc 480

cacagcatca agatctggat cagcttcagc cacgaggacc tgcctagaat gctgcccatc 540

agcagccctc tgcacagacc ccagatgcct ttctggacct gcacctttcc tgccaccaca 600

tggggcacat gggagacacc ttctacatgg ggctggcgga cattcggccc tgtgtactac 660

gtgtgcaacc ggatgaacgt gtccagcgtg ctgaagcctt tcctgttcac cgtgcctatc 720

gaggacatga gagagtgcac cctgggcaga aacagaatga atgtgtcctc tgtgctgaaa 780

ccctgtctga tccccgtgcc aatcctgacc acactgagct tcgtgctgat cgaggaaagc 840

cggcggaaca gacggtacag aaagatctac ggggagctga agatgtccct gcaggccctg 900

cctctgacct ttagcacact gctgtgggac agcagacaca ccaccaccac aagcagcccc 960

agaagatccc ctgctctgag agctagaggc ggcaccacaa tcggcgaagt gacaagcaga 1020

ggcgccagca gctctaccag cagagacagc agaagctttt tcaccagcgc tcaccccagc 1080

agaccctgca gaatgagact gtaccagagc aacggcatgg aagcctgcgg cacacaccct 1140

acacctagac tgtctagagg ccctccactg cacaccagaa ccatgagcct gagcaacatc 1200

ctgatgaagt tccggctgat gctgcagaag cagtgctgct acaacctcgt gatcaagcac 1260

cctctgatct tccggatcaa cctgcggaga cagggcaaga ccaaagacgg cgttgtgcag 1320

agcctgggct tcctgaacca gcagatcatc ctgcagaaag agttcttcat ctgcctgctg 1380

atgttcatca agcggtggaa gccagccgcc acaaagacac ctccaccact gattctgtgg 1440

cctcaggccg ctcctgtgca caagaagaga ggcctgttca gcctgttctt ctcccactgc 1500

ctgtgccacc agatgaagtg cctgaccgcc gtggattgtc tgcaggcctt ctgggacatg 1560

atgattcgca agatccagaa gcggcggaga accgccaacc ggctgaaaaa gaaatacctg 1620

gtgcagtgta ccaacctgag cgtgcccatg atgctgacca tcctgatctg gaagcgggtg 1680

ttcatcctcc tgctgagcga caagaagtgt ctgagagtca gatgcggcgg atggctgtgt 1740

ggctggacac ctagaggcaa gaacaagaag cggagaaagc tgcggcccaa gcacctgaag 1800

aaaaccagcg ccaaggtgtc cgtgatcacc atgaagctct gggagaagca cttcaagggc 1860

ctgaactgga acctggtcgt gtggattctg aacctgaaga tgatgtggct caagaagtcc 1920

accaagagca gacagagcag cccattcctg gctcggagct ggaataccat gcaatggaac 1980

aacaagaaaa tggccatgac cgtgatcgct ggcgcccacc actgtcagta caagctgacc 2040

tacaccaaag tgtggaagat gaattggaag aaaatgatga tcggctccca gcacctcctg 2100

tttagcccac tggattcaag cccactgcag ttgctgctgc accctcacgg caagagatgc 2160

aacccttgct gcagactgac ctggatgagc agagagtggg aaaagcggaa gcagatcctc 2220

ggcgggaggcg gcaagtacaa agagtacttc ctgaagcggga ttctcatccg gaaggctatg 2280

acagtgctgg ccggcgataa gaaaggcctg ggaagattca tgagatgcgt gcagagcgag 2340

acaaaggccg tgtctctgca gctgcctctc ggccgaagaa agtccggcgt gatgaagtgt 2400

tgggatctgt gccccagact ggactttcac cccagatcta gctctggcgg cgagaagctg 2460

aagcactgtt tcaaagcctg gggcctgtgc ttcttctacc tgctgtactt cgagaagaaa 2520

cggcccgagt ggctgagcgt tgaccctcct ggatctgtct ggattctgcc ctgcatggac 2580

ctggacggaa tgctgacagt gatcggccag ggactcgtgg gcatgacccc tagagatctg 2640

ggaacagtgg ctggcgccac cgcacagatt cagaggccta tccaggtgca gacaggcggc 2700

agactgttcc tgattcacct gagaaaggtg gacacccacc atctgcaggt cgtgaccaag 2760

accgtgacag ccacaacagc tgacggccag aggatgcgga gactgaagtg caaggcctgt 2820

cggctgagat gggccagacc tgaacctgct gcacaggctc ctccaaagag ccactttcca 2880

cctcatccta gcggcccctc cagaacctct agcctgcagc tcagacccat tcctccacac 2940

ctgagacacc cactgctgct gaagggaaag aacagcccct gctgccaaaa gaaactgcgg 3000

gtgcagaacc agggccatct gctgatgatc ctgctgcata acgccagaca gccagcagg 3060

3120

agcctgctga cccagccatc tcctgccagc gcatgtacaa tgccaccact cctgcatcca 3180

cagtgggccc cacatagtgc tggactgtct ccaggcgctt gcagacctgc ctgtacctgc 3240

atcagcatga ccaccatctc cagagccaca tggcggctga gaatcccaag cctgaggaag 3300

taccggcaga acatcgagat cacccctcag cggtctaagg cccaggtttc cgtgtacagc 3360

ggcaatctgc tgagcttctc cagacggggc atgaagagta gcgtgcggct gaagaacagc 3420

atccctcacg tgccaaaagc caccgccgct cactctgtga cagtgaagct gcagtacctg 3480

ttttttcctga ccgtgtgcgg cttcgaccgg gtgttcctgg ttaagatctg caagaagaag 3540

ctgctcctca agctgctgag aggccccaga cggaaagtgc tgatggtgct ttggctgccc 3600

gccaaccaga gtctgcagaa aagccaggtg ttccagtggg tgctgcggac cgaaacctac 3660

atggaaatgc actacggcca tcaggcccct caggtggcca tgctgcctaa actgctgtgt 3720

agacagatgc tccccactct gtccacactc cagcagaggc atcctcaggg accttgtcct 3780

cctcctgtga cacctctgag agtgcagtct gtgctgctcc tgggagtgcc tcagacagcc 3840

gcttttagcg gccggattct gcaccagcca gaaatggccg ccatgagact gatgcccaag 3900

ttcctgaact ccaccaacag ctggtggaca gtggccccta gatgtcctag aagcgccgtg 3960

gacattagcc ccggacctac atctcctagc ctggcctctt gtgcctctgt ggccattgga 4020

aaactggcac tgctgggcgc atctgcccct tgtggcgcag ctggtgctag agaggctgga 4080

agatttgccg tgctgggcac catggttatg aagggtgctg ccgcagcctc ctgtcagtct 4140

ccctccatctc caagagagac aacccctagc agcgacgcca ccagacggac aagacctatt 4200

gccgctcaga gcaaaaagac catgactgcc gccggactga cccctcggag aaacctgcag 4260

agaaatctga aggtcctggg cgccaagaag atcaccttcc tgagaaacag cagacgcgcc 4320

acacctacct ctaagggcag cctgagaaga aagtacctga gagtgaacat ccgcaagctc 4380

atctggacca agcagcggag cttcctgtct ctgttcccca agctgagagc caccgagcag 4440

atgaccaatg tgggatgcct gctcaagaaa agcagccaat tgcggcggag ctggcgcaat 4500

gtgtcccggga tcagcaacat ggaacacagg ctgaccatgg acagcgccct gagcctgaca 4560

aagagagagg gccctgagac aagcgagaag aagggaagt tgcctggcta cccctacgat 4620

gtccctgact atgcgtcc 4638

<210> 1194

<211> 4641

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hTPA- and HA-extended polypeptide 4D poly-FSP

<400> 1194

atggatgcaa tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt 60

tcgcccagcc aggaaatcca tgcccgaaac agaaagacca gaaaggccgc cagagagaag 120

caccgcaaga ccgacgacaa gaagaagaag tccaccgaaa agaccaagaa cgccaagaag 180

atgcccttcc agcagcggcc tcctctgcag cctgttaacc tggatctgca gcaccagcgc 240

agacgcaaaa agaagaaaga caaggtcgaa atccggaatc ggagccctaa ggccaaatagc 300

accgagtgtc agggcgagca cagcagagag cagaacctgc tgaatctggt gcagctgaac 360

cctcacagcc ctcaccacag aaaggacgaa gaggaccacc agaagcggca ccaccaccac 420

aatcaaaaga aaatgagcgt gcgggctagc gtgtccttct ggacactttt gccaacaacc 480

ggcgtgcgga caagaggctc tccactgaga ctgacactgt ggcctccacg ggttccacct 540

tctccacctc ttgctctgggg ccccagaatg cagctgtgta cccagctcgc ccggttcttc 600

cctatcacac ctcctgtgtg gcacatcctg ggacctcaga gacacacacc tcaggctcct 660

ctgaggctgt ggtcttggtg cggcacaagc cagacctact tcggcagcag accatctcct 720

tgcctgagct ggtccagcag aattgtggcc cctggacacg ccctgagctg tcggactaga 780

agaagatggt ctagcaagcg gtggatcttc tccaaccagc tgaccgtgca gatgctgtgc 840

ctgccaatca cacacccatt cgcctctcag cctctgggac aagagatgcc caacaacacc 900

cagatccggc tgctgaagcc ctgcagagtg aacgccgtgg aagaactgca cagcaggcgg 960

atgtctgtgc ggatcgtgaa gaactgggtg tcctgctgtt acgtgcggct gtctgctgtg 1020

ggcctgtcta cttggagtgc cctggacgag gaaaagaaga acggccggat cagcagcctg 1080

aaaaagaaag tgaatgcctg cagctccctg atcctgagct tccagcgcgt gaaaaccggc 1140

atggaaatcc tgctgtggat tctgctgaag atggaaattc agatcttcaa gatttgcctg 1200

ggcgagatgc agctctgcgg ccaggccttt ggaagcttta gcgccgattt cagcgcccct 1260

cagatcatta agaagatgaa gaaacggatc atggtgcacg agctgaagaa gaatctgctg 1320

ctgcctatgt gctccagcct ggctctgcca acaggcctga catctctgat cctgcctagc 1380

agcgatctgg ccgtgctgat ctctagctcc accagccact ttctgatgcg gagccctgtg 1440

ctgccaagca gcagactgac ttgtgcctct ccacagctgc cccggatgtg gacttggtct 1500

1560

ctgctcgtcg gctacaacac cagaaacaca gcccagaatc ctcggctgca gcagcacctg 1620

aaacactggc agcagtgcac cagagccaga tggtgtcaag agccctgtca gaccctgacc 1680

ttcttccatt tcttcttctt tttcaaaacg gtgtccctgt gttgccccgg ctggtctaaa 1740

ggacaacctc ctccacctgg cctgaagcct tctagcctgg cttctcaggt ggccagaacc 1800

1860

ggcttctgtc acgtggccca agtgggactc gagagactgg tgtctctgtc cggcatcacc 1920

gacgtgaacc attgggccca gcctaagcca tctcctagca aaggcaccgg catgaacaga 1980

cccatcggcc tgagagttgg caaaggcgtg tgggccagac tgatcctggc catgacactg 2040

accctgaacg gcatggccaa gaagtcccag cagcctctgc aggtcgtgct gagactggga 2100

aacctgcctc cattcttcag aggcagcgag gtgccctttc ctagaccttg tcggaccaag 2160

cctgcctgca caagcaaggc ctggactccc agaaaactcg tggggagagc cgtgcggaga 2220

aagggatctc cactctggga ttgctccacc acaacaggca cacaccacgg atgcctgtgg 2280

gagatcccat ctgtgaccct gctggcccat cctagaaccg cctctatcac cctgcctacc 2340

acaagcctga agggctccag aaccagctgc tgccctagat ctcaccccag actgcctagg 2400

accaaatggc ccctgcagtg tctgctgcac agcctgggaa caaccggaac actcgtttgg 2460

gaaaggggcg tcctgcagat gagatccctc gaagcccatt gtccactgca gaccttccat 2520

ctgcaccact gcaggaccca gcctagctct tgtcctcctg cctggattct gtaccaagga 2580

cagtggagcg gagccgtcgt gggacttaga cctgtggctc accatctgcc tagacctatc 2640

cagggcctgc actgtccaca ggcacctcat caacctgctc tgagctgggg aaaatggccc 2700

gtgggaatga caggccctgc cagatgttgc agatggccca gacggtgcca gaagaaggtt 2760

ccatcagcct ctccatgggg cctcgtgctc aaaaagaacc ggaaacagat gctgaaccag 2820

aagtgtctgg ccccttttag agagagcacc ctgggcaatt tctggatgaa gctgctccag 2880

ctgcctgtcg tgatcaacct gaagagactg cagaagttca acctgcacct gtgctactac 2940

cacagccaga gcaacagaaa caagagccgc cagatggaat ccctgggcat gaagttgcag 3000

3060

ctcggcggaa aagccggacc acaggctcaa gagaaggcca cctctcggat tctgaacctg 3120

caagtgctca agaagatcct gcggagcttt atgaagctgt accactctct ggtcatgtgc 3180

ctgcggctga ggaccaagct ggaaaaggct ctgagcgccc tgttcatctg gccccagcac 3240

agctacaagc ccctgttctt tcacggcgac ctgtgcgagt ggggaggcca ggctcataga 3300

3360

aagaagcaga agtctaacaa gatcatctgc aagggccacc gcatcaccat catctgtatc 3420

aagaatatgt tcctgcagcg caagggcttc ctgaacctga gccacgtgga aaagttctgt 3480

agaggcggcc ctagcgccag cgatagatgc tgttcttgta gccccagcgg cttcagtgcc 3540

ggcagaggaa gatgtcctgt tcagggctgt ctgaggcccc acagagttca actgctgaga 3600

agatggggcc ctggctctcc tgctggacag aggctgtcta agggctttca gctgctccgt 3660

tggtggggac caggatctcc tgctcctgag cctagaaagg gcccatttcc accacctgat 3720

cctccatggc ctgtgaccgc tgtgacagtg atggctggat ctgtgcctag tgctcagtct 3780

gtggacgccc tggaatctcc tggacctctg gctctcgaag gacctagcag ccctagaaac 3840

ctgctttgga gagagatgag catctttctg cccggcatct tcaccgccaa gaaaagcccc 3900

accagctttt gctgcaatat cctgaacagc ctgccaagct ctatggaaat cgccgtgctg 3960

caagaggaca gcaaaaagat gacccagaag ggccaagcct tcggctccct gtgtcagctt 4020

gtgtccgctg acttctctgc ccctcaagtg acccggctgc ggagcctgaa tcatctgctg 4080

atcacctaca caccccacat ctgcagacac accctgctga cccctgacac cagatggatc 4140

tttagcaatc agctgcagac agtccagatg ctctgtctgc ctccactgaa gcagggcgag 4200

agaatcattt ggcccctcgg cggctttcag atccccaact tcttttggag cctcggcatc 4260

ggacacactg gcgcctggtg gcatactgga aggcggagaa gagaatgggg cggcagcacc 4320

gtgtctagaa gccctctgtg gatcagctcc atgatctacc tgagaggcat ggccttcgcc 4380

agcgacagca gcatcagaac caccagagtg tcctggtgca gaagatgcgg cgccaaaagc 4440

gctgccgcca gctctattga agctctgacc gctctgttta cctgccggac cgccagcatc 4500

agcgtgaaca tcagcaccct gatgtccatc ctggaagcac ttaagaagaa acccagagac 4560

atctgccgca tcagctgccg gtgtagacgc gagtacagag tgactatggg ctacccctac 4620

gatgtccctg actatgcgtc c 4641

<210> 1195

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 1C poly-FSP

<400> 1195

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgagca ccagacggga aatgccttct 720

caggccacac cttctccacc tggaccttgg agagccgctc tgggcaaaga agtgaagcgg 780

agccagagca agctgcggct gagaaacaca agccccaccg agatccaatt gaccatgaac 840

gacagcaagc acaagctgga aagccccgct ctgaaacagg tgtcccctgc ctctccacct 900

acacagcagc ctcagacacc ccaggatagc agacaggtgc tggccgcttc ttctcctgga 960

catcctcttg gccctggcga ctgccctaga acaatggctg gaccttctgc ctctctgcac 1020

ccagcatctg gcagagccag cagaaagacc tggcagagaa ggctgagagc ctgtagagct 1080

ggtggacctg ctggatggggc tagacaggcc atgacactga aggccctggt caagacctgg 1140

acacagttca gaaactaccc cagagtcgga gccggaacag gcggaggacc tgcttgtgtt 1200

cctgtggccg aagcttctac tggcgcctgg acagctgaag gcgctgccgg aacaagatgc 1260

agagcttcta gagccccttg cagacagctg cctgccagct ctcctaccaa gtgcgagatc 1320

tgcgtgaaga gaaggcggcg gagaaagcgg agcagccagt ctagatctcc cgtgcctaag 1380

aaacgcgagc ggatgatgct gcctgtgcgg gccagaacag ctagatctgc tctgccacaa 1440

ggcagagccc ctctggaagg aatgcttctg cctagagcac tgcccagcct gctgagcaga 1500

atgccagcca gaagagagag ccggttttgg ctggcactgc aggctctgggg actcagacct 1560

cctcatctgc ctagctgtca ccctctgcct caactgcttg gacctcctcc acctcatcag 1620

gcccctttcg acaagaaagg cttcgtgatc ctgagcaagg gcaagaagcg cgggaaagaa 1680

atccggcgga tgaccagacg gaccatgacc accaccaaaa tgatgatgat gcggcggacc 1740

aagcggcgga ggcgaaaacg tagaagaatg accatgatga cccgcagaac cctgctgacc 1800

aagaaaacct gtctgacccc tagaaccagc gccgccacaa aagctctgcc cagacagaca 1860

atcagccagc ggaggcctat ccagacacct cctggaacaa gcctggccat ctggggcaga 1920

ctgcctctca gaccacagag aggacctcgg cctctgcata gatggctgca gcagacaaca 1980

ggctgctccc agagaagaat cgccaagaag cggatcaagc tgaggcggaa cacactggcc 2040

atccggttta tctctgcccc tcagcctagc agcagcaccg ttacagctgc tcctagcggc 2100

cagtacttca gcagcttctt ctacaccagc gaccggacca agtgtgaaat ctgtgtcaag 2160

aacaccgcca tcctggctat caccctgatc acccctcagg accctcacct ggaccctgtg 2220

tgtcacccac atagaaggcc tggctggaag ggccaccaca gagtggtgtt tagccaccat 2280

ccaagcggct ccaccaagag ccattggcag cagagagaaa gaggcgggagg ccgcagagaa 2340

aaaaagggct gcagagagat gcccagcagc tggtctagct ctggcagaac cgccgacaaa 2400

aagaacgtgc ggagcagaac cagactgacc ctgacaggca caagaagcag cggcacaaga 2460

gcctctccaa gcgccgtgtg tctgtgctgt tggacatgcc cctccagaag cttccctgcc 2520

gcatctccat ctggactgag agcacctgtt ggagcccagg ctcctcctga agtgacactg 2580

agagccagct gtgcctctag cgcctctaaa gcctgtgctg gactttggcc ttggggagcc 2640

caagtgcgga acaaagtgcg gcagaacttc aagcggaacc tggccaaaaa gaccctgact 2700

aacgccctgc ggagtatgcc caagagcaga aaccaggtgc tgttcagacg gaatctgacc 2760

ccaagccagc tgagaactct ggcccctcct tacatgctgc tgccccagtc tctgtctcag 2820

agcctgtcca gaaagcagat ccccagccaa tcaatgctcg tgaccgcctt cttcctgctc 2880

gacatcatcc tgagaagcct gctgtactcc cggatgtgcc tggtgcagat ccctacacac 2940

acctgggagc ccgactactt cgtgtccctg cctatgatgt tcagctgggc cactcagagc 3000

tttccagtgg gagccgtgtt cctgaccaga cctgccattc cttgctgcag ctgtaccagc 3060

cacagcaacc cttggagcca gtccttcaag aagaagaagc ccctgacatt cagccccagc 3120

gtgccaatgt ctctggcctg tagcatcacc gtgtccttcg tgggccagat cagcacactg 3180

gtgctggacg tgtcctccat ctctcactgg cgcaagcgca agagagagaa gcggtcccag 3240

aagtcccggc agaaccatct gcagctgaag tcctgcagac ggaagatcag caactggtcc 3300

aagctgagat ccccgtggat cttctaccct cggctgaaaa ccttcggcgt gccactgggc 3360

tctatcctgt gtctggcagg ctccctgagc acgatgatga tcattagcac caccagacaa 3420

3480

ccttggcgga catctgctat gccatctgcc atgggagtcg ccctgcctac cagctgtgaa 3540

agcggaagat ctcctcctgc caccggcgga agaatgcctg gatctcagtc tatcatgatg 3600

agctggatgc ctccactggc tccctgtgcc gcttgtcctt gttctcctgc tcctacactg 3660

tgccctgctc atcctgcaag agcccctaca gccgtgcctg cctttacacc tctgtctgct 3720

caccctgtgc cagtgctgtc tggatgtcac ctggccgtgc gcacctctat gctgacactg 3780

ctgcccatga gacaccctca cagaagccca cctacaagct gccctccact gctggaagct 3840

ttcccactgc caagggccag cctgagagct accgtgccta gacacagctc tgtgctggcc 3900

actggactgc atctgcatcg gcagattagc ctgcctctga ggggcttcct gctgcatcaa 3960

cctctgcctg gacaacacct gtggctgtcc cacagactga ggctgagact gccaagaaca 4020

ggcgagaaga acgacctcca gctgttcgtg atgagcgaca ccacatacaa gatctactgg 4080

acagtgatcc tgctgaaccc ctgcggcaac ctgcacctga aaaccacatc tctgcggaga 4140

gccagaccta cctcctgcag cacatgcaga atgtggtggc tgaagctggc cccactgcaa 4200

gtgaccacaa cactgaagct gatcctggtc atgctggaaa tcgtgaccga agaggccaag 4260

aaatccatga agagatcccg gcggagcaag atcgaggcct ctcctatcgg ctccatcatg 4320

aagaagaaag acagcaccat cttcatcaac cagcagctca agtggacccc tcacatcctg 4380

aagtctgcca gcgtgtccaa gaagagactg cccaagctga acggcatctg ggaagtgatg 4440

ccccggacac tggaaggctg catcagcgtg aaggaccacc ggacaagcca gaaccacagc 4500

aagtgcatca cggagtgtgt gcggacagcc ctgaaccaga gaaaggtggt caagaacccc 4560

agccagccag tgccactgag aaacgacggc atcaccaacg gcaacgactc caccagatcc 4620

tgctccatca gcctgaagaa aagcctggaa aagatgcccc tgatgaacgc caagaaaatg 4680

gaagatatga tctgggagag caacatgggc ggcaaccggt ccatctgcct gacaagactg 4740

ccctgcaaag tgaccgagtc cagccaaaga ccacctcgga gcattctgtc cctggcctct 4800

agagccggac agtttcccgt ttggcggaga accagcagag gcatgtccct gcagtggaag 4860

tccggcaatg cctgcacacc tctgaggtgc cctagcagaa gccctgccac atttctgacc 4920

gcactgaacg gctggcacag atctggactt agggctcagc aacctagcct gccaggacct 4980

tgtgctagcc tgctgtctac actgagccag cctccaccac aggctccaag ccaagtttgg 5040

acagccgcca cactgagatg tcccgctgtt cctgcagctg cttgccctcc tatcgtgttc 5100

tttctggccc catacttccc cacaccactg agcatcagca gacccacctc tcgggccaga 5160

cagtcttgtt gtctccccgg actgacacac cctgctcacc agcctcttgg ctctatgggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1196

<211> 5232

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV - and HA-extended polypeptide 2C poly-FSP

<400> 1196

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgcggc agatcaacag acggaagctg 720

cagagaaaga agcgccggaa gaagagagag gaccactgcg gactgcgggc cagaaaccgg 780

aacatgaaga aaaccatgag ccacctgatc gcccatcagg ccctgaagat gtctggcctg 840

agactgtttc accccagact gctgacacgg aagaagcctg gcaagaaccc tcggagaaag 900

acctggaaga tgcggaagaa gtacgcccag aagcctagcc agaagaacca cctgtacggc 960

aagggcctga cacaccctgt gcctctgaag agaaccctgc agaacaagcg gaacaagaac 1020

atcaagcgcg tgaaccagaa gaagggcaac atcaagagaa agagcatcct gctgcacctc 1080

atcgtgctga acagccccga gagcaacctg caggctcatc ctcaagttcc agcactggct 1140

cctcaggctt ggtggcctgc tagaagaggc cctctgacaa gcgctccatc tgctcagcag 1200

agcctgacct tcctgcctag cagatttcct tggggccctc ctccactgca ctggaactct 1260

gcctacctgg gcagaacact gctcgtgacc tgcatcctgt accacagatg gatcgtgacc 1320

agcatgtgcc agagccagag atggctgacc agcaccacct ctagaagcag cgccctgatg 1380

tggacccagt ggtctagcgt gacaagcctg gaatggacag cccaagagag gggctgtagc 1440

agctggctga tgaagcagac ctggatgaag tcttggagcc tgcgggaccc cagctacaga 1500

tccatcctgg aatacgtgtc caccagagtg ctgtggatgc ccacatctac agtgggagcc 1560

ctgagagcca cagctcctgc aacagctgct ccacaggctc agacccctca ttggagagcc 1620

gccacaacaa gcagcatgag cagcagccct agagccgagc ctacaagctc tagctcttgg 1680

gccacctgtt ggccgatg ttgcagctcc agctgttact ggtccctgtc ctcttggcct 1740

cctgctggtg ctgccgaggc cacaaatacc aggaccagaa gcagagagtc ccagcggggc 1800

1860

cggacctcca gcagacaccc ttcttggcct tggaccagat gcctgagaat gcggcctcct 1920

agaagcaaca gccggaaaaa gctgctcgag gacatgaagc aaaatggctt cacccggtac 1980

aagaaacggg gcgtgatagc ctggcggaga gccactttcc tgctggccct ttgggagtgc 2040

agtctgcccc aagctagact gtgcctgatc gtgtctagga ccctgctgct ggtccagagc 2100

agatgcagcc tgatggaaca gatccctcac ctgaagtcct ggtggtgtac aagaggcggc 2160

2220

tgtccttgga agggcatctg ctggaagtgg cctgtgacag tggccgctct gatcctgatt 2280

cagcacggcg gcatcttcac cttccggttc tgggtcgaga agctgtcctg gaacatctgc 2340

aagaatctgt ccctgtacct ggacaagttc gtgcagcggc tgctgtacgt gtacatcatc 2400

gagacaaacg tgttcgtcct gaacctgttc cacgtgccag tgaatcagat cttcatgatg 2460

gccttctacc tgaagtacac cgagaaggcc tgggtcatgg aactggaatg gctcattcag 2520

cagcagtgct accagttgct gctggctcat atgtacggct tcagcaccta cggcacccag 2580

aaaggcgagt gcgtgtgtct gcagtgtctg agcggcagct ccctgttcat cctgaagcag 2640

ccactggacc tgaagatcag caagaccttc atgagcagat cccacctgga tttcagcccc 2700

tattggatga agaaagtgaa ggcctctcca cagatcatgc agcggatgag cgccgtgaac 2760

ttcgagacaa tcaggcggaa gtggcagatc cggcacgtgc tgaagaacct gcctctggaa 2820

aacacacaga tccccaggac aagccctctg tggaagatca caatggtgct gacatacccc 2880

aactgcgtgg tgcatgtgct gatgttcgtg ctgttcaagg ccgaccagag cgagtctagc 2940

ctgagcagcg gaatctaccc tcctagcacc tgtagcctgc cttgccgggg cattagaatg 3000

tgcgccctgt ctcaccctag cttctacatc agcagcaccg tgtgctttct gagcaagcac 3060

tgcatgaccg cctgcaccag agagaagatc atcaacccca ccatcagctg cccctttcag 3120

agccccacaa agagactgggg ccacagagag ggcagcgaga gcgataatct gggcctgctg 3180

tactcctttc ctccttggct gcccatgaga tccctgggcg aaccttggag agctggcttt 3240

cagtggggag agtccccttg gcaagtgatg gctagcctga ccggcaacag cgtgatcgtg 3300

cacaagatcg tgctggtgtt tctgaagtct ctgagcagct acaactgcga gcggcggatc 3360

tctggcatcc agaagaggc catcgagttc gccgtcctga agctgctgaa catggacctg 3420

gctagccctc agattatgca gagaaccagc gccgaccggt tccggatgaa gcggagaatg 3480

cagaacctgg ataacctggg cgtgccctgg ccattgcttc caggtgctgc tcctccacat 3540

agactgctgg gcccctctag atggattctg tttcatgtgc ggaacatcgt gtacggcatc 3600

caagtgatgc tgtgccggcg gagaaacagc agaaagcaga gtcaggtgca gctggccatt 3660

3720

ggactgacca tgaccaccgg ccggaacatc aacagcaact ccctgatgac caactgcctg 3780

ttcagcccca tcttcctgtg ccaccacgcc atcatgcacc ggaagatgac aagcccacac 3840

ttcagactgt tctccagcaa gatcccacat cctcaggtgc caagtgtggt ggccctgtgc 3900

aagttcctgg ccaatggcct gtggttcaac ctgatctttc agaaaaaggg ccacatgatc 3960

tttagcccca agttcttcct gacctttagc cagcggaccc tggaatccgc cggactgttt 4020

ccatggctgc acacccagtg cgggaacaaa aagaccaagc tgtggttttc cctgatcaac 4080

atccaccacc gcaagaatcc tctgctgcct atgagaatga gcagaacacc tcctcggaga 4140

gtgtgcggat ctccacctct gcatctgtgc caacctctga gaggcggcaa gaagagaagc 4200

aagttcgcct tctggaagcc tcctagccag ggcatcagct cttgtctgat gacaggctgt 4260

tggggctcct gtggcagagc ttgttcttgc ggaagaggct gcggtacacc cagaggcgac 4320

tggaaaagac acccacctcg cagagagctg agcctggaac catggattcc ttatctgcac 4380

cagcaaaaga cccagcggaa gcggatgaag accggcgcca ttcaagagaa gggcgctatc 4440

caagaaaaag gcgcaatgac caagcggatc tgcgtggtca atttcgaggc ctgcagagag 4500

atggaaagcc tgagcgcccc tgagaagatt accctgttcg aagcccacat ggcccggtgc 4560

acctccatca atgtgctgtg tgtccggggcc agcctgattg agaagctgat gaaagaaaag 4620

cgcaagatga agcgcaacca ggtggcatcc cctcaaatca tgcaacggct gttcatcaag 4680

ggctaccaga acaaccactt cacgttccac ctccaaaact ttgccttctt cagctccttc 4740

ggcaacaaca agtatatcaa cattctcacc cgggccttca tccacacacc tagaagccct 4800

ggctgttccg tgtacgccaa tatgtgctgg cggaagatgc agtccctgaa tcaccccagc 4860

agactgacca actgtcaccc cgcttggctg agccccatta gtgcttgtcc taggctgctg 4920

ccctatcctt ttcacgtgct ggcactggaa gtgacctttc cacctggcac agccatgcct 4980

cagatgcaat tgcaggcact ggctatgtgg gagaaaggct gggacaagca agagaagaaa 5040

tcccgcaagc tgatgtcctc ctgcctggaa aaaaaggcta cctggatgcg gagaacccct 5100

ggcacaagcc tgcagtgtta cctgaacaca cacccctgca ccatgctgct gcctcaggga 5160

atcccatctc tgaacgccca gtggatgttc ctgcggatcg gctaccccta cgatgtccct 5220

gactatgcgt cc 5232

<210> 1197

<211> 5247

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 3C poly-FSP

<400> 1197

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgccta ttcctccaca cctgagacac 720

cctctgctgc tgaagaacaa gaagcggaga aagctgcggc ccaagagcag aaagaagggc 780

cacaccaaga accggcggaa ggccaagaga aagccccaga tcagaaacag ccgcagagcc 840

acacctacca gcaagggcag cctgagaaga aagtacctga gagtgggagc cgccgctgct 900

agctgtcaat ctcctccatc tcctagagag acaaccccta gcagcgacgc caccagaaga 960

accagaccta tcgccgctca gagcaagaaa accatgacag ccgccggact gacccctagc 1020

cagaagaaag atcctgccgg caagctgaag aaacaggtgt ccgtgtacag cggcaacctg 1080

ctgagcttct ctcggagagg catgaagtct agcgtgcgcc tgaagaactg caccaacctg 1140

agcgtgccca tgatgctgac catcctgatc tggaagcggg tgttcatcct gctgctgtcc 1200

gacaagaaat ctatccctca cgtgcccaag gccaccgccg ctcattctgt gacagtgaag 1260

ctgcagagcc ggcggaacag acggtacaga aagatctatg gcgagctgaa gatgtccctg 1320

caggccagca atggcatgga agcctgtggc acacacccca cacctagact gtccgtgtcc 1380

gtgatcacca tgaagctgtg ggagaagcac ttcaagggcc tgaactggaa cctggtcgtg 1440

tggattctga acctgaagat gatgctcaag aagaagtatc tggtccagcg cgagtgggag 1500

1560

ctcatccgca aggccatgac agtgctggcc ggcgataaga aaggcctgggg cagattcatg 1620

agatgcgtgc agagcgagac aaaggccgtc agtctgcagc tgcctctggg cagaatggac 1680

ctggatggaa tgctgaccgt gatcggccag ggactcgtgg gcatgacacc tagagatctg 1740

ggaacagtgg ctggcgccac cgcacagatt cagaggccta ttcaggtgca gacaggcggc 1800

agactgagag gccctagaag aaaggtgctg atggtgctgt ggctgcccgc caatcagagc 1860

ctgcagaaaa gccaggtgtt ccagtgggtg ctgagaaccg agctgcccct gacctttagc 1920

accctgctgt gggacagcag acacgacctg cctagaatgc tgcccatcag cagccctctg 1980

cacagacccc agatgccttt ctggacctgc acctttcctg ccaccacatg gggcacatgg 2040

gagacacctt ctacatgggg ctggcggacc tttgccagac agccttctag acccaccggc 2100

agaaccccta ccaccagact gatggctcca gtgggcagcg tgatgagctg tagcctgctg 2160

acacagccat ctccagccag cgcctgtaca atgcctccac tgctgcatcc tcagtgggcc 2220

cctcattctg ctggactttc tccaggcgct tgcagacctg cctgtacctg catcagcatg 2280

accacaatca gcagggccac ctggcgcaga aacctgcaga gaaacctgaa agtgctgcgg 2340

ctgagaatcc ccagcctgcg gaagtacaga cagcagaaca cccggtccac caacctcgag 2400

aagaacccat ctctgaagaa gcagagccag aggcggggca agctgtctct gaccacactg 2460

agcttcgtgc tgatcgagga actgctgaaa aagagcagcc agctgcggag aagctggcgg 2520

aacgtgtcca gaatcagcaa catccggaag ctgatttgga ccaagcagcg gagcttcctg 2580

tctctgttcc ccaagctgag agccaccgag cagatgacca atgtgggctg tagactggat 2640

tttcacccca gaagcagctc cggcgacaga gtgttcctgg tcaagatcca gagcagccca 2700

ttcctggcca gatcctggaa taccatgcag tggaacaaca agaaaatggc catgactgtg 2760

atcgctggcg cccaccactg tcagtacaag ctgacctaca ccaaagtgtg gaagatgaat 2820

tggaagaaaa tgatgatcgg cagccagcac ctcctgttct ccccactgga tagctctcca 2880

ctccagctgc tgctgcaccc acacggcaag agatgcaacc cctgttgtcg gctgacatgg 2940

atgagccacc tgaatcggaa gctgcacctc accaggtcct gcctgatcga cctgaccatg 3000

atgatgagcc agaaactgtg gaagaatcag cggaagcgca tccctctgcc ttctccaaga 3060

cagcatctgc tgcctcagta tcctctgcac cctcctccac cttgtctgct gcttctccac 3120

catagacacc acctggcatt cctcgagatg gcctgtagct ctcagcacac ccacagcatc 3180

aagatctgga tcagcttcag ccacgagaca atgagcctgt ccaacatcct gatgaagttc 3240

cggctgacca ccaccacaag cagccccaga agaagccctg ctctgcgagc tagaggcggc 3300

acaacaatcg gcgaagtgac cagcagcctg ttctttagcc actgcctgtg cgtggcccct 3360

agatgtccta gatctgccgt ggacatcagc cccggaccta catctccttc tctggcctct 3420

tgtgcctctg tggccattgg aaagctggca ctgcttggcg cctctgctcc ttgtggtgct 3480

gctggcgcta gagaggccgg aagatttgct gtgctgggca ccatggtcat gaagaacatg 3540

3600

ggccccgtgt actacgtgtg caaccggatg aatgtgtcca gcgtgctgaa gcctttcctg 3660

ttcaccgtgc ctatcgagga catgagagag tgcaccctgg gacgcaacag aatgaatgtc 3720

tcttccgtgc tgaaaccctg tctgatcccc gtgccaatct gcaagaagaa actgctgctc 3780

aagctgctga ggggcgcctc cagctctacc agcagagata gcagaagctt tttcaccagc 3840

gctcacccca gcagaccctg caggatgaga ctgtaccaga tgctgcagaa gcagtgctgc 3900

tacaacctcg tgatcaagca ccctctgatc ttccggatca acctgcggag acagggcaag 3960

accaaagacg gcgttgtgca gagcctgggc ttcctgaacc agcagatcat cctccagaaa 4020

gagttcttca tctgcctgct gatgttcatc aagcggtgga agccagccgc caccaagaag 4080

ggcaagaaca gcccctgctg ccagaaaaaa ctgcgggtgc agaaccaggg ccatctgctg 4140

atgattctgc tgcacaacag aggcccacct ctgcatacca gattcgagaa aaagcggccc 4200

gagtggctga gcagaaaatc cggcgtgatg aagtgctggg atctgtgccc tgccttcagc 4260

ggcaggattc tgcaccagcc agaaatggcc gccatgagac tgatgcccaa gttcctgaac 4320

tccaccaaca gctggtggac agtgcacaag aagagaggcc tgttccacca gatgaagtgt 4380

ctgaccgccg tggactgtct gcaggccttc tgggatatga gcgagaagaa acgcaagctg 4440

cctatgattc gcaaaatcca gaagcggcgg agaaccgcca accggaacat cgagatcacc 4500

cctcagagaa gcaaagccca cctgaagaaa acctccgcca agggcgccaa gaaaatcacc 4560

ttcctgtgcc tgagagtcag atgcggaggc tggctgtgtg gctggacacc aagaggatgg 4620

ctgaagaagt ccaccaagtc cagactgtgc ttcttctacc tgctgtacta cctgtttttc 4680

ctgaccgtgt gcggcttcac ccctccacca cttattctgt ggcctcaggc agcccctggc 4740

gagaagctga agcactgttt taaagcctgg ggcgtcgacc ctcctggcag cgtttggatt 4800

ctgccctgct tcctgattca cctgagaaag gtggacaccc accacctcca ggtggtcacc 4860

aaaacagtga ccgccacaac agccgacggc cagaggatga gaaggctgaa gtgcaaggcc 4920

tgccggctga gatgggctag acctgaacct gcagctcagg cccctcctaa gagccacttt 4980

ccacctcatc ctagcggccc ctccagaacc agctctctgc aattgagaac ctacatggaa 5040

atgcactacg gccatcaggc cccacaggtg gcaatgctgc ctaaactgct gtgtcggcag 5100

atgctcccca ctctgtctac cctgcagcag agacatcctc agggcccatg tcctcctcca 5160

gtgacacctc tgagagtgca gtctgtgctg ctgctcggag tgcctcaaac agctggctac 5220

ccctacgatg tccctgacta tgcgtcc 5247

<210> 1198

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV- and HA-extended polypeptide 4C poly-FSP

<400> 1198

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatggaca gcaagaagat gacccagaag 720

aaccgcaaga ccgggaaggc cgccagagag aagcacagaa agaccgacaa cctggacctg 780

cagcaccagc gcagacgcaa aaagaagaag gacaaggtcg agatccggaa tcggagccct 840

aaggccaata gcaccgagtg tcagggcgag cacagcagag agcagaacct gctgaacctg 900

gtgcaattga accctcacag ccctcaccac cggaaggacg aggaagatca ccagaagaga 960

caccaccacc acaatcaaaa gaagatgtcc gtcaacgagg gcaacaacca ctggcctaga 1020

gtggaaatgc ctaccggctg gctgctcgtg ggctacaaca ccagaaacac agcccagaat 1080

cctcggctgc agcagcacct gaaacactgg cagcagtgca ccagagccag atggtgtcaa 1140

gagccctgtc agaccctgac cttcttccac ttcttcttct ttttcaaaac ggtgtccctg 1200

tgctgccccg gctggtctaa aggacaacct cctccacctg gcctgaagcc ttctagcctg 1260

gcttctcagg tggccagaac cacaggcgtg taccagcacg tgtggctgat ctgcttcgtg 1320

tttttcgtgt gcgtggaaac cggcttctgt cacgtggccc aagtgggact cgagagactc 1380

gttagcctga gcggcatcac cgacgtgaac cattgggccc agcctaagcc atctcctagc 1440

aaaggcaccg gcatgaacag acccatcggc ctgagagttg gcaaaggcgt gtgggctaga 1500

ctgtggctga agaactggaa gcggttcaag ctcaaaaaga aatacacagg catggaaatc 1560

1620

cagctgtgcc tcaagaagaa agtcaacgcc tgcagcaacg ccaagaaaat gcccttccag 1680

tgccggtgca gacgcgagta cagagtgacc atgattcaga agaagaaaca gaagtccccg 1740

ctgttcttcc acggcgacct gtgcagacct tctccttgcc tgtcttggag cagcaggatc 1800

gtggctcctg gacacgccct gagctgtcgg actcgtagaa gatggtccag caagatggcc 1860

aagaagtccc agcagcctct gtgtagaggc ggcccttctg ccagcgatag atgctgtagc 1920

tgtagcccca gcggctttag cgctggaaga ggcagatgtc ctgtgcaggg atgtctgagg 1980

ccacacagag ttcagctgct gagaagatgg ggccctggat ctcctgctgg ccagagactg 2040

tctaagggct tccaactgct gcgttggtgg ggaccaggat ctccagctcc tgagcctaga 2100

aagggcccat ttccaccacc tgatcctcca tggcctgtga ccgctgtgac agtgatggct 2160

ggctctgtgc ctagcgctca gtctgtggat gctctggaat cccctggacc tctggctctc 2220

gaaggcccta gcagccctag aaacctgctc tggcgggaaa tgagcatctt cctgcctggc 2280

atcttcaacc tgcacctgtg ctactaccac agccagagca accggaacaa gagccggcag 2340

atggaatccc tgggcatgaa gctgcagatc acacacccat tcgccagcca gccactgggc 2400

caagagatgc ccaacaacac ccagatcaga ctgctgaagc ccggccaggc ctttggcagc 2460

ttttccgccg atttttcagc ccctcagcag gcccctctga gactgtggtc ttggtgcggc 2520

acaagccaga ccctacttcgg ctctatcgaa gccctgacag ccctgttcac ctgtagaacc 2580

gccagcatca gcgtgaacat cagcaccctg atgagcatcc tggaagccat cctggccatg 2640

acactgaccc tgaatggcag ccctctgtgg gactgcagca caacaacagg cacacaccac 2700

ggctgcctgt gggagatccc ttctgttacc ctgctggcta ccgccaaaaa gagccctacc 2760

agcttctgct gtcagcggcc tcctctgcag ccagtggaag agaagaagaa cggccggatc 2820

agcagcgtgc caccttctcc acctcttgct ctgggcccta gaatgcagct ctgcacccag 2880

ctggcccggt tcttccctat tacacctcct gtgtggcaca tcctgggacc tcagagacac 2940

acccctctgc tgacccctga caccagatgg atcttctcca accagctgca gaccgtgcag 3000

atgctgtgtc tgcctccact gaagcagggc gagagaatca tctggcctct cggcggcttt 3060

cagatcccca acttcttttg gagcctcggc atcggacaca ctggcgcctg gtggcatact 3120

3180

tccatgatct acctgagagg catggccttc gcctccgaca gcagcatcag aaccaccaga 3240

gtgtcctggt gtcggagatg tggcgccaaa agcgccgcag ccagcagcga caagaagaag 3300

3360

gtgctgagac tgggcaacct gcctccattc ttcagaggca gcgaggtgcc ctttcctaga 3420

ccttgccggga ccaagtgcca gaagaaggtt ccaagcgcct ctccttgggg actgcaccca 3480

agaacagcct ccatcacact gcctaccacc agcctgaagg gcagcagaac aagctgctgc 3540

cccagatctc accccagact gcccagaaca aagtggcctc tgcagtgcct gctgcacagc 3600

3660

gctcactgcc ctctgcagac attccacctc caccactgca gaacccagcc tagctcttgt 3720

cctccagcct ggattctgta ccaaggacag tggagcggag ccgtcgtggg acttagacct 3780

gtggctcacc atctgccaag acctatccag gggctgcact gtcctcaggc tcctcatcaa 3840

ccagctctgt cctggggaaa gtggcccgtg ggaatgacag gacccgccag atgttgccgg 3900

tggcctagaa gaatgtgtag ctctctggcc ctgccaaccg gcctgacatc tctgatcctg 3960

cctagcagtg atctggccgt gctgatctct agctccacca gccactttct gatgcggagc 4020

ccagtgctgc caagcagcag actgacatgt gcctctccac agctgcctcg gatgtggaca 4080

tggtctagct ggctgaaaga gtggggagga caggcccaca gacagcggaa ctgtagagtg 4140

aacgccgttg aggaactgca cagcaggcgg atgtctgtgc ggatcgtgaa gaattgggtg 4200

tcctgctgtt acgtgcggct gagtgcagtg ggactgtcta cttggagcgc cctggattcc 4260

ctgatcctgt ccttccagag agtgaaaacc agccggatac tgaacctcca ggtgctgaag 4320

aagatcctgc ggagctttat gaagctgtac cactctctcg tgatgtgcct gcggctgcgg 4380

accaagctgg aaaaagctct gagcgctctg tttatctggc cccagcacag ctacaaaggc 4440

caggcattcg gaagcctgtg ccagctggtg tctgccgatt tcagcgcccc tcaagtgacc 4500

agactgcgga gcctgaacca tctgctgatt cggtggatct ttagcaatca gctgacagtc 4560

caaatgctct gcctgccgat catcaagaaa atgaagaaac ggatcatggt gcacgtcttg 4620

aagaagaatc ggaagcagat tatctgtatc aagaatatgt tcctgcagcg gaagggattc 4680

ctgaacctga gccacgtgga aaagttcaga gagagcaccc tgggcaattt ctggatgaag 4740

ctcctgcagc tgccaccagc ttgcacctct aaggcctgga cacccagaaa gcttgtgggg 4800

agagccgtgc ggagaaaggg cgtcgtgatc aacctgaaga gactgcagaa attccgggct 4860

agcgtgtcct tctggactct gctgcctaca acaggcgtgc ggacaagagg ctctccactg 4920

agactgactc tgtggcctcc aagaagccag aaaagccagc accactgcgc cctgagaaag 4980

atcgagtggg ccacaaagct gtccctcggc ggcaaagcag gaccacaggc tcaagagaag 5040

gccaacatcc tgaacagcct gccttcctct atggaaatcg ccgtgctgca agaaatgctg 5100

aaccagaagt gtctggcccc tttcgagctt aagaagaacc tcctgctgcc aacctacaca 5160

ccccacatct gtcggcacac ccttaaaaag aagcccagag acatctgcag aatcagcggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1199

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV - and HA-extended polypeptide 1D poly-FSP

<400> 1199

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatggccg acaagaaaaa cgtgcggagc 720

gctagttctc ctggacatcc tcttggcccc agaagaatga cggacggac catgaccacc 780

accaaaatga tgatgatgcg gcggaccaag cggcggagaa gaaaaagacg ccggatgacc 840

atgatgacca gaagaaccct gctgaccaag aaaacctgtc tgacccctag aaccagcgcc 900

gccacaaaag ctctgcccag acagacaatc agccagcggga ggcctatcca gacacctcct 960

ggaacaagcc tggccatctg gggcagaaga agcaagatcg aggcctctcc tatcggcagc 1020

atcacccctc tgagcatcag cagacctacc agcagagcca gacagagctg ttgcctgcct 1080

ggactgacac accctgctca tcagcctctg ggctctatgg ttggagctgg aacaggcgga 1140

ggacctgctt gtgtgcctgt ggccgaagct tctacaggcg cttggacagc tgaaggcgct 1200

gccggcacaa gatgtagagc cagcagagcc ccttgcagac agctgcctgc ttctagccct 1260

1320

tctcccgtgc ctaagaaacg cgagcggatg atgctccctg tgcggggccag aacagctaga 1380

tctgctctgc cacaaggcag ggctcctctg gaaggaatgc ttctgcctag agcactgccc 1440

agcctgctga gcagaatgcc tgccagaaga gagagcagat tctggctggc cctgcaggcc 1500

cttggactca gacctcctca tctgcctagc tgtcaccctc tgcctcaact gcttggacct 1560

cctccacctc atcaggcccc tggcgagaaa aacgacctgc agctgttcgt gatgagcgac 1620

accacctaca agatctactg gaccgtgatc ctgctgaacc cctgcggcaa cctgcacctg 1680

aaaaccacca gcctgaacgc caagaaaatg gaagatatga tctgggagag caaccagcag 1740

ctgaagtgga cccctcacat cctgaagtcc gcctctatcg ccaagaagcg gatcaagctg 1800

cggcggaaca gcaccagacg ggaaatgcct tctcaggcca caccttctcc acctggacct 1860

tggagagccg ctctgggcaa agaagtgaag cggagccaga gcaagctgag actgagaaac 1920

acaagcccct tcgacaaaaa gggctttgtg atcctgagca acatgggcgg caaccggtct 1980

atctgcctga ccatcgtgtt ctttctggcc ccttacttcc ccatgcctag cagctggtct 2040

agcagcggca gaaccagact gcctctgagg ccacagagag gccctagacc tctgcataga 2100

tggctgcagc agaccaccgg ctgctctcag agaagaggcg actgcccaag aaccatggcc 2160

ggaccatctg cttctctgca ccctgcatct ggcagagcct ccagaaagac ctggcagaga 2220

aggctgagag cctgtagagc aggcggacct gctggatggg ctagacaggc cagaaaacgc 2280

aagagagaga agcggtccca gaagtccaga cagaaccatc tgcagctgaa aagctgcaga 2340

cggaagatca gcaactggtc taagctgcgg agcccctgga tcttcgaggc caaaaagagc 2400

atgaagagat cccagcggcc tcctcggagc atcctgagcc tgttcaagaa gaagaagccc 2460

ctgacattca gccccagcgt gtccaagaag aggctgccta agctgaaggg caagaaacgc 2520

cggaaagaaa tccagcggaa ggtggtcaag aaccccagcc agcctgtgcc actgagaaat 2580

gacggcatca ccaacggcaa cgacagcacc cggtcttgca gcatcagcct gaagaaaagc 2640

ctggaaaaga tgcccctgat gcggagcctg ctgtactcca gaatgtgcct ggtgcagatc 2700

cccacacaca cctgggagcc cgactacttt gtgtccctgc ctatgatgtt cagctgggcc 2760

acacagagct ttccagtggg cgccgtgttt ctgaccagac ctgccattcc ttgctgtagc 2820

tgtaccagcc actccaatcc ttggagccag tccacactgg ccatccggtt tatctctgcc 2880

cctcagccaa gcagcagcac cgttacagct gctcctagcg gccagtattt tgccctgcgg 2940

agtatgccca agagccggaa ccaggtgctg tttcggagaa atctgacccc aagccagctg 3000

cggacactgg cccctccata tatgctgctg cctcagtctc tgtcccagag cctgtctcgg 3060

aagcagatcc catctcagtc catgctcgtg cggaccaggc tgacactgac aggcactaga 3120

agctccggca ccagagcctc tccatctgcc gtgtgtctgt gctgctggac atgccccagc 3180

agaagctttc ctgccgcctc tccaagcgga ctgagagcac ctgttggagc acaggctcct 3240

ccagaagtga cactgagagc cagctgtgcc agctccgcca gcaaagcttg tgcaggactt 3300

tggccttggg gagcccaagt gcggatgaag aagaaggaca gcaccatctt catctacccc 3360

aggctgaaaa ccttcggcgt gccactggggc agcattctgt gtctggctgg aagcctgagc 3420

acgatgatga tcattagcac caccagacaa gagtgcggca gacgggccag cgtgccctgt 3480

agacctatga tcggaagcgc cagacctgga ccatggcgga caagtgctat gccttctgcc 3540

atgggagtcg ccctgcctac cagctgtgaa agcggaagat ctcctcctgc caccggcgga 3600

agaatgccag gatctcagtc tatcatgatg agctggatgc ctccactggc tccctgcgct 3660

gcttgtcctt gttctcctgc tcctacactg tgccctgctc accctgctag agcaccaaca 3720

gccgtgcctg cctttacacc cctgtctgct catcctgtgc cagtgctgtc tggatgtcac 3780

ctggctgtgc ggacctccat gcttaccttg ctgccaatga tgaccctgaa ggccctggtc 3840

aagacatgga cccagttcag aaactacccc agacggcacc ctcacagaag cccaccaaca 3900

agctgtcctc cactgctcga ggcctttcca ctgccaagag ccagcctgag agctaccgtg 3960

cctagacact ctagcgtgct ggccacagga ctgcatctgc ataggcagat tagcctgcct 4020

ctgcggggct tcctgctgca tcaaccactg ccaggacaac acctgtggct gtcccacaga 4080

ctgagactgc ggctgcctag aacaaccgag atccagctga ccatgaacga ctccaagcac 4140

aagctggaaa gccccgctct gaaacaggtg tccccagcta gtcctcctac acagcagcct 4200

cagacacccc aggattctag acaggtgctg gccgtgccta tgagcctggc ctgtagcatc 4260

accgtgtcct tcgtgggcca gatctctacc ctggtgctgg acgtgtcctc catcagccac 4320

tggaacggca tctgggaagt gatgcccaga acactggaag gctgcatctc cgtgaaggac 4380

caccggacat cccagaacca cagcaagtgc atcaccgagt gtgtgcggac agccctgaac 4440

accaagtgtg aaatttgtgt gaagaacacc gccatcctgg ctatcaccct gatcacccca 4500

caggaccctc acctggaccc tgtgtgtcat ccccatagaa ggcccggctg gaagggacac 4560

cacagagtgg tgtttagcca ccatccttcc ggcagcacca agtctcactg gcagcagaga 4620

gaaagaggcg gaggccgcag agaaaagaag ggctgtagag aggcaagcag agccggccag 4680

tttcctgtgt ggcggagaac atctagaggc atgtccctgc agtggaagtc cggcaatgcc 4740

tgcacacctc tgagatgtcc cagcagatct ccagccacct tcctgactgc cctgaacggc 4800

tggcatagat ctggactgag ggcccagacc gccttctttc tgctggacat catcctgcgg 4860

agagcccggc ctacaagctg cagcacatgt agaatgtggt ggcggctgcc ctgcaaagtg 4920

accgaaagct ccctggccaa gaaaaccctg acccaatcagc ctagcttgcc cggaccttgt 4980

gctagcctgc tgtctacact gagccagcca ccacctcagg ctcctagcca agtttggaca 5040

gccgctacac tgcggtgtcc tgctgtgcct gctgctgcat gtccacctct gaagctggct 5100

ccactgcaag tgaccacaac actgaagctg atcctggtca tgctggaaat cgtgaccgag 5160

aacaaagttc ggcagaactt caagcggaac tccagcttct tctacaccag cgacagaggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1200

<211> 5232

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV - and HA-extended polypeptide 2D poly-FSP

<400> 1200

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgcgga agatgcagag cctgaatcac 720

cccagcagac tgaccaacaa tcctcggaga aagacctgga agatgcggaa gaagtacgcc 780

cagaagccta gccagaagaa ccacctgtac cggcagatca accggcgcaa gctgcagaga 840

aagaagtcca gaagccacct ggacttcagc ccctactggga tgaagaaagt gaagcgggcc 900

agaaaccgga acatgaagaa aaccatgagc cacctgatcg cccatcaggc cctgaagatg 960

tctggcctga gactgtttca ccccagactg ctgaccagaa agaagcccgg caagcagaaa 1020

aagggccaca tgatcttcag ccggcggaag tggcagatcc ggcacgtgct gaagaatctg 1080

atccccagcc tgaacgccca gtggatgttc ctgagaatcg ctagcccaca gatcatgcag 1140

agaaccagcg ccgaccggtt ccggatgaag cggagaatgc agaacctgga caatctgggc 1200

gtgccctggc ctctgttgcc tggattgctg ctggcccata tgtacggctt cagcacctac 1260

ggcacccaga aaggcgagtg cgtgtgtctg cagtgtctga gcggcagcag cctgttcatc 1320

ctgaagcagc ccctggacct gaagatctcc aagaccttca tggccagccc tcagattatg 1380

cagcggatga gcgccgtgaa cttcgagaca atcctgcagg cccatcctca ggtgccagct 1440

cttgctcctc aagcttggtg gcccgctaga agaggccctc tgacatctgc tccaagcgca 1500

cagcagtccc tgacacggac actggaaagc gccggactgt ttccttggct gcacacccag 1560

tgccgggcct tcattcacac acctagaagc cctggctgca gcgtgtacgc caacatgtgc 1620

tggcggtaca agaaacgggg agtgatcgcc tggcggatct accctcctag cacatgcagc 1680

ctgccttgca gaggcctgat cctgattcag cacggcggca tcttcacctt ccggttctgg 1740

gtcgagaagc tgagctggaa catctgcaag aacctgagcc tgtacctgga taagttcgtg 1800

cagcggctgc tgtacgtgta catcatcgag acaaacgtgt tcgtgctgaa cctgttccac 1860

gtgccagtga accagatctt catgatggcc ttctacctga agtacaccga gaaggcctgg 1920

gtcatggaac tggaatggct gatccagcag cagtgctacc agcggaagag aatgaagacc 1980

ggcgccatcc aagagaaggg cgctattcaa gagaaagggg ccatgaccaa gcggatctgc 2040

gtggtcaatt tcgaggcctg cagagagatg gaaagcctga gcgcccctga gaagatcacc 2100

ctgtttgaag cccacatggc ccggtgcacc agcatcaatg tgctgtgtgt cagggccagc 2160

ctgatcgaga aactgatgaa agaaaagcgc aagatgaagc gcaaccaggt ggcatcccct 2220

caaatcatgc aacggctgtt catcaagggc taccagaaca accacttcac gttccacctc 2280

2340

ctgcccagca gattcccttg gggacctcct ccactgggag cccttagagc tacagctcct 2400

gctacagctg ctccccaggc acagacacct cattggagag ccgccaccac aagcagcatg 2460

agcagctctc caagagccga gcctaccagc tctagctctt gggccacttg ctggcctaga 2520

tgctgcagct ccagctgcta ttggagcctg tctagctggc ctcctgctgg cgctgccgaa 2580

gccacaaata ccagaaccag aagccgcgag agccagcggg gaagaatgct gacttggaga 2640

agctccctgt ggctgcaggg caccagatgt tttacaggcg tgcggaccag ctccaggtgc 2700

agcctgatgg aacagatccc tcacctgaag tctaacagcc ggaagaaact gctcgaggac 2760

2820

gccatccagg tgctgctggt ggacaagcac aagtactttc tgttcggcag agccacattt 2880

ctgctggctc tgtgggagtg tagcctgcct caggctagac tgtgcctgat cgtgtctaga 2940

accctgctgc tcgtgcagtc tagacaccca agctggcctt ggaccagatg cctgagaatg 3000

cggcctccac gcagccactg gaactctgcc tacctgggga gaacactgct cgtgacctgc 3060

atcctgtacc acagatggat cgtgaccagc atgtgccaga gccagagatg gctgacctcc 3120

accacctcta gaagctctgc cctgatgtgg acctggtggt gtacaagagg tggcggcaag 3180

3240

tggaagggca tctgttggaa gtggcctgtg acagtggccg ccatgagcag aacacctcca 3300

agacgcgtgt gcggccacag agaggggcagc gagtctgata atctgggcct gctgtactcc 3360

tttccacctg tgctgttcaa ggccgaccag agcgaaagct ctctgagcag cggcaacagc 3420

gtgatcgtgc acaagatcgt gctggtgttc ctgaagtccc tgagcagcta caactgcgag 3480

agaagaatca gcggcatcca gaagagcc atcgagttcg ccgtcctgaa gctgctgaac 3540

atggacctcg gcaagggact gacacacccc gtgcctctga agaggaccct gcagaacaag 3600

cggaacaaga acatcaagcg ggtcaaccag aagaagggca acatcaagag gaagtccatc 3660

ctgctgcatc tgattgtgct gaacagcccc gagagcaacc gggaaaagat catcaacccc 3720

accatcagct gcccctttca gagccccaca aagagactgg gaagccctcc tctgcacctg 3780

tgtcagcctc tgaggccta tcctatcagc gagggcctga ccatgaccac cggcagaaac 3840

atcaacagca actccctgat gaccaactgc ctgttcagcc ccatcttcct gtgccaccac 3900

gccatcatgc accggaagat gacaagccct cacttcagac tgttctccag caagatccca 3960

catccacagg tcccaagtgt ggtggccctg tgcaagttta cacacccctg caccatgctg 4020

ctgccccaag gcgctttttg gaagcctcct agccagggca tctccagctg tctgatgaca 4080

ggctgttggc agtggtccag cgtgacaagc ctggaatgga cagcccaaga gagaggctgt 4140

tcctcctggc tgatgaagca aacatggatg aagtcttggt ccctgcggga ccccagctac 4200

agatccatcc tggaatacgt gtccacacgg gtgctgtgga tgcccacctc taccgtgcct 4260

aagttctttc tgaccttcag ccagatccgg atgtgcgccc tgtctcaccc tagcttctac 4320

atcagctcca ccgtgtgctt tctgagcaag cactgcatga ccgcctgcac acctggaacc 4380

agcctgcagt gttacctgaa cctggccaac ggcctgtggt tcaacctgat cttccggaac 4440

aaaaagacca agctgtggtt ctccctgatt aacatccacc accgcaagaa cccactgctg 4500

cccatgcggc tggaaaaaaa ggccacttgg atgcggcgga ccgagctgtc tctggaacct 4560

tggattcctt acctgcatca gcagaaaacc cagtggctcc ccatgagaag cctgggcgag 4620

ccttggcgag ctggatttca gtggggagag tctccctggc aagtgatggc ctctctgaca 4680

ggcagacgca agaaaagaga ggatcactgc ggactgcaag agaaaaaaag ccggaagctg 4740

atgtccagct gcgccgctcc tccacataga ctgctgggac ctagcagatg gggaagctgt 4800

gggagagcct gttcttgtgg cagaggctgt ggaaccccta gaggcgactg gaagagacac 4860

ccacctcggc ggattctgtt tcacgtgcgg aacatcgtgt acggaatcca agtgatgctg 4920

tgctgtcacc ccgcctggct gtctccaatc agcgcttgtc ctcgcctgct gccttatcca 4980

ttccacgttc tggccctgga agtgacattc cctccaggca ctgccatgcc tcagatgcaa 5040

ttgcaggcac tggcaatgtg ggagaaaggc tgggacaagc ctctggaaaa cacacagatc 5100

cccaggacaa gcccactgtg gaagatcaca atggtgctga cataccccaa ctgcgtggtg 5160

cacgtcctga tgttcggcgg caagaagcgg agcaagttcg gctaccccta cgatgtccct 5220

gactatgcgt cc 5232

<210> 1201

<211> 5247

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV - and HA-extended polypeptide 3D poly-FSP

<400> 1201

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgcaga acaccagaag caccaacctg 720

gaaaagaacc ccagcctgaa gaagcagagc cagcggagag gaaagctgag cagcagaaag 780

aagggccaca ccaagaaccg gcggaaggcc aagagaaagc cccagatcag ccagaagaag 840

gaccctgccg gcaagctgaa gaaacacctg aaccggaagc tgcacctgac cagatcctgc 900

ctgatcgacc tgaccatgat gatgagccag aagctgtgga agaaccagcg caagagaatc 960

cctctgcctt ctccacggca gcatctgctg cctcagtatc cactgcatcc tccacctcca 1020

tgcctgctgc tgctccacca cagacaccat ctggcctttc tcgagatggc ctgcagcagc 1080

cagcacaccc acagcatcaa gatctggatc agcttcagcc acgaggacct gcctagaatg 1140

ctgcccatca gcagccctct gcacagaccc cagatgcctt tctggacctg cacctttcct 1200

gccaccacat ggggcacatg ggagacacct tctacatggg gctggcggac attcggccct 1260

gtgtactacg tgtgcaaccg gatgaacgtg tccagcgtgc tgaagccttt cctgttcacc 1320

1380

gtgctgaaac cctgtctgat ccccgtgcca atcctgacca cactgagctt cgtgctgatc 1440

gaggaaagcc ggcggaacag acggtacaga aagatctacg gggagctgaa gatgtccctg 1500

caggccctgc ctctgacctt tagcacactg ctgtgggaca gcagacacac caccaccaca 1560

agcagcccca gaagatcccc tgctctgaga gctagaggcg gcaccacaat cggcgaagtg 1620

1680

caccccagca gaccctgcag aatgagactg taccagagca acggcatgga agcctgcggc 1740

acacacccta cacctagact gtctagaggc cctccactgc acaccagaac catgagcctg 1800

agcaacatcc tgatgaagtt ccggctgatg ctgcagaagc agtgctgcta caacctcgtg 1860

atcaagcacc ctctgatctt ccggatcaac ctgcggagac agggcaagac caaagacggc 1920

gttgtgcaga gcctgggctt cctgaaccag cagatcatcc tgcagaaaga gttcttcatc 1980

tgcctgctga tgttcatcaa gcggtggaag ccagccgcca caaagacacc tccaccactg 2040

attctgtggc ctcaggccgc tcctgtgcac aagaagag gcctgttcag cctgttcttc 2100

tcccactgcc tgtgccacca gatgaagtgc ctgaccgccg tggattgtct gcaggccttc 2160

tgggacatga tgattcgcaa gatccagaag cggcggagaa ccgccaaccg gctgaaaaag 2220

aaatacctgg tgcagtgtac caacctgagc gtgcccatga tgctgaccat cctgatctgg 2280

aagcgggtgt tcatcctcct gctgagcgac aagaagtgtc tgagagtcag atgcggcgga 2340

tggctgtgtg gctggacacc tagaggcaag aacaagaagc ggagaaagct gcggcccaag 2400

cacctgaaga aaaccagcgc caaggtgtcc gtgatcacca tgaagctctg ggagaagcac 2460

ttcaagggcc tgaactggaa cctggtcgtg tggattctga acctgaagat gatgtggctc 2520

aagaagtcca ccaagagcag acagagcagc ccattcctgg ctcggagctg gaataccatg 2580

caatggaaca acaagaaaat ggccatgacc gtgatcgctg gcgcccacca ctgtcagtac 2640

aagctgacct acaccaaagt gtggaagatg aattggaaga aaatgatgat cggctcccag 2700

cacctcctgt ttagcccact ggattcaagc ccactgcagt tgctgctgca ccctcacggc 2760

aagagatgca acccttgctg cagactgacc tggatgagca gagagtggga aaagcggaag 2820

cagatcctcg gcggaggcgg caagtacaaa gagtacttcc tgaagcggat tctcatccgg 2880

aaggctatga cagtgctggc cggcgataag aaaggcctgg gaagattcat gagatgcgtg 2940

cagagcgaga caaaggccgt gtctctgcag ctgcctctcg gccgaagaaa gtccggcgtg 3000

atgaagtgtt gggatctgtg ccccagactg gactttcacc ccagatctag ctctggcggc 3060

gagaagctga agcactgttt caaagcctgg ggcctgtgct tcttctacct gctgtacttc 3120

gagaagaaac ggcccgagtg gctgagcgtt gaccctcctg gatctgtctg gattctgccc 3180

tgcatggacc tggacggaat gctgacagtg atcggccagg gactcgtggg catgacccct 3240

agagatctgg gaacagtggc tggcgccacc gcacagattc agaggcctat ccaggtgcag 3300

acaggcggca gactgttcct gattcacctg agaaaggtgg acacccacca tctgcaggtc 3360

gtgaccaaga ccgtgacagc cacaacagct gacggccaga ggatgcggag actgaagtgc 3420

aaggcctgtc ggctgagatg ggccagacct gaacctgctg cacaggctcc tccaaagagc 3480

cactttccac ctcatcctag cggcccctcc agaacctcta gcctgcagct cagacccatt 3540

cctccacacc tgagacaccc actgctgctg aagggaaaga acagcccctg ctgccaaaag 3600

aaactgcggg tgcagaacca gggccatctg ctgatgatcc tgctgcataa cgccagacag 3660

cccagcaggc ctaccggaag aacccctacc acaagactga tggcccctgt gggcagcgtg 3720

atgtcttgta gcctgctgac ccagccatct cctgccagcg catgtacaat gccaccactc 3780

ctgcatccac agtgggcccc acatagtgct ggactgtctc caggcgcttg cagacctgcc 3840

tgtacctgca tcagcatgac caccatctcc agagccacat ggcggctgag aatcccaagc 3900

ctgaggaagt accggcagaa catcgagatc acccctcagc ggtctaaggc ccaggtttcc 3960

gtgtacagcg gcaatctgct gagcttctcc agacggggca tgaagagtag cgtgcggctg 4020

aagaacagca tccctcacgt gccaaaagcc accgccgctc actctgtgac agtgaagctg 4080

cagtacctgt ttttcctgac cgtgtgcggc ttcgaccggg tgttcctggt taagatctgc 4140

aagaagaagc tgctcctcaa gctgctgaga ggccccagac ggaaagtgct gatggtgctt 4200

tggctgcccg ccaaccagag tctgcagaaa agccaggtgt tccagtgggt gctgcggacc 4260

gaaacctaca tggaaatgca ctacggccat caggcccctc aggtggccat gctgcctaaa 4320

ctgctgtgta gacagatgct ccccactctg tccacactcc agcagaggca tcctcaggga 4380

ccttgtcctc ctcctgtgac acctctgaga gtgcagtctg tgctgctcct gggagtgcct 4440

cagacagccg cttttagcgg ccggattctg caccagccag aaatggccgc catgagactg 4500

atgcccaagt tcctgaactc caccaacagc tggtggacag tggcccctag atgtcctaga 4560

agcgccgtgg acattagccc cggacctaca tctcctagcc tggcctcttg tgcctctgtg 4620

gccattggaa aactggcact gctgggcgca tctgcccctt gtggcgcagc tggtgctaga 4680

gaggctggaa gatttgccgt gctgggcacc atggttatga agggtgctgc cgcagcctcc 4740

tgtcagtctc ctccatctcc aagagagaca acccctagca gcgacgccac cagacggaca 4800

agacctattg ccgctcagag caaaaagacc atgactgccg ccggactgac ccctcggaga 4860

aacctgcaga gaaatctgaa ggtcctgggc gccaagaaga tcaccttcct gagaaacagc 4920

agacgcgcca cacctacctc taagggcagc ctgagaagaa agtacctgag agtgaacatc 4980

cgcaagctca tctggaccaa gcagcggagc ttcctgtctc tgttccccaa gctgagagcc 5040

accgagcaga tgaccaatgt gggatgcctg ctcaagaaaa gcagccaatt gcggcggagc 5100

5160

agcctgacaa agagagaggg ccctgagaca agcgagaaga agaggaagtt gcctggctac 5220

ccctacgatg tccctgacta tgcgtcc 5247

<210> 1202

<211> 5250

<212> DNA

<213> Artificial sequence

<220>

<223> Polynucleotide encoding hINV - and HA-extended polypeptide 4D poly-FSP

<400> 1202

atgcatcgac ggcgctcacg gagttgccgg gaagaccaaa agcctgtgat ggacgatcag 60

cgggacctta tttctaacaa tgaacaactt ccgatgcttg gtaggcggcc tggagcacca 120

gagtccaagt gttctaggggg cgccctgtac actggtttct ccatcctcgt gacactgttg 180

ctggcgggac aggcaacaac tgcgtatttc ttgtatcagc agcaaggaag actggataag 240

cttacagtta catcccagaa tctccaactt gaaaacctca gaatgaagct tccgaagccc 300

ccaaagcccg tctccaaaat gaggatggcg acaccgctcc ttatgcaagc ccttcccatg 360

ggggctctgc cccaagggcc aatgcagaac gctacaaagt atggaaatat gacagaggat 420

catgtcatgc atttgcttca aaacgctgat ccccttaaag tttaccctcc tctgaagggg 480

agctttccag aaaatcttcg acatctcaag aacacgatgg aaacgattga ctggaaggtg 540

tttgagagct ggatgcacca ttggctcctg ttcgagatgt cccggcattc tctggagcaa 600

aagccgacgg acgcacctcc caaggaaagc ctggaattgg aggacccgag tagtgggctg 660

ggcgtcacaa agcaagacct gggacccgtg ccgatgaaca gaaagaccag aaaggccgcc 720

agagagaagc accgcaagac cgacgacaag aagaagaagt ccaccgaaaa gaccaagaac 780

gccaagaaga tgcccttcca gcagcggcct cctctgcagc ctgttaacct ggatctgcag 840

caccagcgca gacgcaaaaa gaagaaagac aaggtcgaaa tccggaatcg gagccctaag 900

gccaatagca ccgagtgtca gggcgagcac agcagagagc agaacctgct gaatctggtg 960

cagctgaacc ctcacagccc tcaccacaga aaggacgaag aggaccacca gaagcggcac 1020

caccaccaca atcaaaagaa aatgagcgtg cgggctagcg tgtccttctg gacacttttg 1080

ccaacaccg gcgtgcggac aagaggctct ccactgagac tgacactgtg gcctccacgg 1140

gttccacctt ctccacctct tgctctgggc cccagaatgc agctgtgtac ccagctcgcc 1200

cggttcttcc ctatcacacc tcctgtgtgg cacatcctgg gacctcagag acacacacct 1260

caggctcctc tgaggctgtg gtcttggtgc ggcacaagcc agacctactt cggcagcaga 1320

ccatctcctt gcctgagctg gtccagcaga attgtggccc ctggacacgc cctgagctgt 1380

cggactagaa gaagatggtc tagcaagcgg tggatcttct ccaaccagct gaccgtgcag 1440

atgctgtgcc tgccaatcac acacccattc gcctctcagc ctctgggaca agagatgccc 1500

aacaacaccc agatccggct gctgaagccc tgcagagtga acgccgtgga agaactgcac 1560

agcaggcgga tgtctgtgcg gatcgtgaag aactgggtgt cctgctgtta cgtgcggctg 1620

tctgctgtgg gcctgtctac ttggagtgcc ctggacgagg aaaagaagaa cggccggatc 1680

agcagcctga aaaagaaagt gaatgcctgc agctccctga tcctgagctt ccagcgcgtg 1740

aaaaccggca tggaaatcct gctgtggatt ctgctgaaga tggaaattca gatcttcaag 1800

atttgcctgg gcgagatgca gctctgcggc caggcctttg gaagctttag cgccgatttc 1860

agcgcccctc agatcattaa gaagatgaag aaacggatca tggtgcacga gctgaagaag 1920

aatctgctgc tgcctatgtg ctccagcctg gctctgccaa caggcctgac atctctgatc 1980

ctgcctagca gcgatctggc cgtgctgatc tctagctcca ccagccactt tctgatgcgg 2040

agccctgtgc tgccaagcag cagactgact tgtgcctctc cacagctgcc ccggatgtgg 2100

acttggtcta gctggctgaa gaacgagggc aacaaccact ggcctagagt ggaaatgcct 2160

accggctggc tgctcgtcgg ctacaacacc agaaacacag cccagaatcc tcggctgcag 2220

cagcacctga aacactggca gcagtgcacc agagccagat ggtgtcaaga gccctgtcag 2280

accctgacct tcttccattt cttcttcttt ttcaaaacgg tgtccctgtg ttgccccggc 2340

tggtctaaag gacaacctcc tccacctggc ctgaagcctt ctagcctggc ttctcaggtg 2400

2460 gccagaacca caggcgtgta ccagcacgtg tggctgatct

gtggaaaccg gcttctgtca cgtggcccaa gtgggactcg agagactggt gtctctgtcc 2520

ggcatcaccg acgtgaacca ttgggcccag cctaagccat ctcctagcaa aggcaccggc 2580

atgaacagac ccatcggcct gagagttggc aaaggcgtgt gggccagact gatcctggcc 2640

atgacactga ccctgaacgg catggccaag aagtcccagc agcctctgca ggtcgtgctg 2700

agactgggaa acctgcctcc attcttcaga ggcagcgagg tgccctttcc tagaccttgt 2760

cggaccaagc ctgcctgcac aagcaaggcc tggactccca gaaaactcgt ggggagagcc 2820

gtgcggagaa agggatctcc actctgggat tgctccacca caacaggcac acaccacgga 2880

tgcctgtggg agatcccatc tgtgaccctg ctggcccatc ctagaaccgc ctctatcacc 2940

ctgcctacca caagcctgaa gggctccaga accagctgct gccctagatc tcaccccaga 3000

ctgcctagga ccaaatggcc cctgcagtgt ctgctgcaca gcctgggaac aaccggaaca 3060

ctcgtttggg aaaggggcgt cctgcagatg agatccctcg aagcccattg tccactgcag 3120

accttccatc tgcaccactg caggacccag cctagctctt gtcctcctgc ctggattctg 3180

taccaaggac agtggagcgg agccgtcgtg ggacttagac ctgtggctca ccatctgcct 3240

agacctatcc agggcctgca ctgtccacag gcacctcatc aacctgctct gagctgggga 3300

aaatggcccg tgggaatgac aggccctgcc agatgttgca gatggcccag acggtgccag 3360

aagaaggttc catcagcctc tccatggggc ctcgtgctca aaaagaaccg gaaacagatg 3420

ctgaaccaga agtgtctggc cccttttaga gagagcaccc tgggcaattt ctggatgaag 3480

ctgctccagc tgcctgtcgt gatcaacctg aagagactgc agaagttcaa cctgcacctg 3540

tgctactacc acagccagag caacagaaac aagagccgcc agatggaatc cctgggcatg 3600

3660

aagttgtctc tcggcggaaa agccggacca caggctcaag agaaggccac ctctcggatt 3720

ctgaacctgc aagtgctcaa gaagatcctg cggagcttta tgaagctgta ccactctctg 3780

gtcatgtgcc tgcggctgag gaccaagctg gaaaaggctc tgagcgccct gttcatctgg 3840

ccccagcaca gctacaagcc cctgttcttt cacggcgacc tgtgcgagtg gggaggccag 3900

gctcatagac agcggaactg gctgaaaaac tggaagcggt tcaagcttaa aaagaagtac 3960

atccagaaga agaagcagaa gtctaacaag atcatctgca agggccaccg catcaccatc 4020

atctgtatca agaatatgtt cctgcagcgc aagggcttcc tgaacctgag ccacgtggaa 4080

aagttctgta gaggcggccc tagcgccagc gatagatgct gttcttgtag ccccagcggc 4140

ttcagtgccg gcagaggaag atgtcctgtt cagggctgtc tgaggcccca cagagttcaa 4200

ctgctgagaa gtggggccc tggctctcct gctggacaga ggctgtctaa gggctttcag 4260

ctgctccgtt ggtggggacc aggatctcct gctcctgagc ctagaaaggg cccatttcca 4320

ccacctgatc ctccatggcc tgtgaccgct gtgacagtga tggctggatc tgtgcctagt 4380

gctcagtctg tggacgccct ggaatctcct ggacctctgg ctctcgaagg acctagcagc 4440

cctagaaacc tgctttggag agagatgagc atctttctgc ccggcatctt caccgccaag 4500

aaaagcccca ccagcttttg ctgcaatatc ctgaacagcc tgccaagctc tatggaaatc 4560

gccgtgctgc aagaggacag caaaaagatg acccagaagg gccaagcctt cggctccctg 4620

tgtcagcttg tgtccgctga cttctctgcc cctcaagtga cccggctgcg gagcctgaat 4680

catctgctga tcacctacac accccacatc tgcagacaca ccctgctgac ccctgacacc 4740

agatggatct ttagcaatca gctgcagaca gtccagatgc tctgtctgcc tccactgaag 4800

cagggcgaga gaatcatttg gcccctcggc ggctttcaga tccccaactt cttttggagc 4860

ctcggcatcg gacacactgg cgcctggtgg catactggaa ggcggagaag agaatggggc 4920

ggcagcaccg tgtctagaag ccctctgtgg atcagctcca tgatctacct gagaggcatg 4980

gccttcgcca gcgacagcag catcagaacc accagagtgt cctggtgcag aagatgcggc 5040

gccaaaagcg ctgccgccag ctctattgaa gctctgaccg ctctgtttac ctgccggacc 5100

gccagcatca gcgtgaacat cagcaccctg atgtccatcc tggaagcact taagaagaaa 5160

cccagagaca tctgccgcat cagctgccgg tgtagacgcg agtacagagt gactatgggc 5220

tacccctacg atgtccctga ctatgcgtcc 5250

<210> 1203

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA Cloning Cassette 1C

<400> 1203

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaagcacc 120

agacgggaaa tgccttctca ggccacacct tctccacctg gaccttggag agccgctctg 180

ggcaaagaag tgaagcggag ccagagcaag ctgcggctga gaaacacaag ccccaccgag 240

atccaattga ccatgaacga cagcaagcac aagctggaaa gccccgctct gaaacaggtg 300

tcccctgcct ctccacctac acagcagcct cagacacccc aggatagcag acaggtgctg 360

gccgcttctt ctcctggaca tcctcttggc cctggcgact gccctagaac aatggctgga 420

ccttctgcct ctctgcaccc agcatctggc agagccagca gaaagacctg gcagagaagg 480

ctgagagcct gtagagctgg tggacctgct ggatgggcta gacaggccat gacactgaag 540

gccctggtca agacctggac acagttcaga aactacccca gagtcggagc cggaacaggc 600

ggaggacctg cttgtgttcc tgtggccgaa gcttctactg gcgcctggac agctgaaggc 660

gctgccggaa caagatgcag agcttctaga gccccttgca gacagctgcc tgccagctct 720

cctaccaagt gcgagatctg cgtgaagaga aggcggcgga gaaagcggag cagccagtct 780

agatctcccg tgcctaagaa acgcgagcgg atgatgctgc ctgtgcgggc cagaacagct 840

agatctgctc tgccacaagg cagagcccct ctggaaggaa tgcttctgcc tagagcactg 900

cccagcctgc tgagcagaat gccagccaga agagagagcc ggttttggct ggcactgcag 960

gctctgggac tcagacctcc tcatctgcct agctgtcacc ctctgcctca actgcttgga 1020

ccctcctccac ctcatcaggc ccctttcgac aagaaaggct tcgtgatcct gagcaagggc 1080

aagaagcgcc ggaaagaaat ccggcggatg accagacgga ccatgaccac caccaaaatg 1140

atgatgatgc ggcggaccaa gcggcgggagg cgaaaacgta gaagaatgac catgatgacc 1200

cgcagaaccc tgctgaccaa gaaaacctgt ctgaccccta gaaccagcgc cgccacaaaa 1260

gctctgccca gacagacaat cagccagcgg aggcctatcc agacacctcc tggaacaagc 1320

ctggccatct ggggcagact gcctctcaga ccacagagag gacctcggcc tctgcataga 1380

tggctgcagc agacaacagg ctgctcccag agaagaatcg ccaagaagcg gatcaagctg 1440

aggcggaaca cactggccat ccggtttatc tctgcccctc agcctagcag cagcaccgtt 1500

acagctgctc ctagcggcca gtacttcagc agcttcttct acaccagcga ccggaccaag 1560

tgtgaaatct gtgtcaagaa caccgccatc ctggctatca ccctgatcac ccctcaggac 1620

cctcacctgg accctgtgtg tcacccacat agaaggcctg gctggaaggg ccaccacaga 1680

gtggtgttta gccaccatcc aagcggctcc accaagagcc attggcagca gagagaaaga 1740

ggcgggaggcc gcagagaaaa aaagggctgc agagagatgc ccagcagctg gtctagctct 1800

ggcagaaccg ccgacaaaaa gaacgtgcgg agcagaacca gactgaccct gacaggcaca 1860

agaagcagcg gcacaagagc ctctccaagc gccgtgtgtc tgtgctgttg gacatgcccc 1920

tccagaagct tccctgccgc atctccatct ggactgagag cacctgttgg agcccaggct 1980

cctcctgaag tgacactgag agccagctgt gcctctagcg cctctaaagc ctgtgctgga 2040

ctttggcctt ggggagccca agtgcggaac aaagtgcggc agaacttcaa gcggaacctg 2100

gccaaaaaga ccctgactaa cgccctgcgg agtatgccca agagcagaaa ccaggtgctg 2160

ttcagacgga atctgacccc aagccagctg agaactctgg cccctcctta catgctgctg 2220

ccccagtctc tgtctcagag cctgtccaga aagcagatcc ccagccaatc aatgctcgtg 2280

accgccttct tcctgctcga catcatcctg agaagcctgc tgtactcccg gatgtgcctg 2340

gtgcagatcc ctacacacac ctgggagccc gactacttcg tgtccctgcc tatgatgttc 2400

agctgggcca ctcagagctt tccagtggga gccgtgttcc tgaccagacc tgccattcct 2460

tgctgcagct gtaccagcca cagcaaccct tggagccagt ccttcaagaa gaagaagccc 2520

ctgacattca gccccagcgt gccaatgtct ctggcctgta gcatcaccgt gtccttcgtg 2580

ggccagatca gcacactggt gctggacgtg tcctccatct ctcactggcg caagcgcaag 2640

agagagaagc ggtcccagaa gtcccggcag aaccatctgc agctgaagtc ctgcagacgg 2700

aagatcagca actggtccaa gctgagatcc ccgtggatct tctaccctcg gctgaaaacc 2760

ttcggcgtgc cactgggctc tatcctgtgt ctggcaggct ccctgagcac gatgatgatc 2820

attagcacca ccagacaaga gtgtggcaga cgggcctccg tgccttgtag acctatgatc 2880

ggcagcgcta gacccggacc ttggcggaca tctgctatgc catctgccat gggagtcgcc 2940

ctgcctacca gctgtgaaag cggaagatct cctcctgcca ccggcggaag aatgcctgga 3000

3060 tctcagtcta tcatgatgag ctggatgcct

tctcctgctc ctacactgtg ccctgctcat cctgcaagag cccctacagc cgtgcctgcc 3120

tttacacctc tgtctgctca ccctgtgcca gtgctgtctg gatgtcacct ggccgtgcgc 3180

acctctatgc tgacactgct gcccatgaga caccctcaca gaagcccacc tacaagctgc 3240

cctccactgc tggaagcttt cccactgcca agggccagcc tgagagctac cgtgcctaga 3300

cacagctctg tgctggccac tggactgcat ctgcatcggc agattagcct gcctctgagg 3360

ggcttcctgc tgcatcaacc tctgcctgga caacacctgt ggctgtccca cagactgagg 3420

ctgagactgc caagaacagg cgagaagaac gacctccagc tgttcgtgat gagcgacacc 3480

acatacaaga tctactggac agtgatcctg ctgaacccct gcggcaacct gcacctgaaa 3540

accacatctc tgcggagagc cagacctacc tcctgcagca catgcagaat gtggtggctg 3600

aagctggccc cactgcaagt gaccacaaca ctgaagctga tcctggtcat gctggaaatc 3660

gtgaccgaag aggccaagaa atccatgaag agatcccggc ggagcaagat cgaggcctct 3720

cctatcggct ccatcatgaa gaagaaagac agcaccatct tcatcaacca gcagctcaag 3780

tggacccctc acatcctgaa gtctgccagc gtgtccaaga agagactgcc caagctgaac 3840

ggcatctggg aagtgatgcc ccggacactg gaaggctgca tcagcgtgaa ggaccaccgg 3900

acaagccaga accacagcaa gtgcatcacc gagtgtgtgc ggacagccct gaaccagaga 3960

aaggtggtca agaaccccag ccagccagtg ccactgagaa acgacggcat caccaacggc 4020

aacgactcca ccagatcctg ctccatcagc ctgaagaaaa gcctggaaaa gatgcccctg 4080

atgaacgcca agaaaatgga agatatgatc tgggagagca acatgggcgg caaccggtcc 4140

atctgcctga caagactgcc ctgcaaagtg accgagtcca gccaaagacc acctcggagc 4200

attctgtccc tggcctctag agccggacag tttcccgttt ggcggagaac cagcagaggc 4260

atgtccctgc agtggaagtc cggcaatgcc tgcacacctc tgaggtgccc tagcagaagc 4320

cctgccacat ttctgaccgc actgaacggc tggcacagat ctggacttag ggctcagcaa 4380

cctagcctgc caggaccttg tgctagcctg ctgtctacac tgagccagcc tccaccacag 4440

gctccaagcc aagtttggac agccgccaca ctgagatgtc ccgctgttcc tgcagctgct 4500

tgccctccta tcgtgttctt tctggcccca tacttcccca caccactgag catcagcaga 4560

cccacctctc gggccagaca gtcttgttgt ctccccggac tgacacaccc tgctcaccag 4620

cctcttggct ctatgggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1204

<211> 4672

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 2C cloning cassette

<400> 1204

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgacggcag 120

atcaacagac ggaagctgca gagaaagaag cgccggaaga agagagagga ccactgcgga 180

ctgcgggcca gaaaccggaa catgaagaaa accatgagcc acctgatcgc ccatcaggcc 240

ctgaagatgt ctggcctgag actgtttcac cccagactgc tgacacggaa gaagcctggc 300

aagaaccctc ggagaaagac ctggaagatg cggaagaagt acgcccagaa gcctagccag 360

aagaaccacc tgtacggcaa gggcctgaca caccctgtgc ctctgaagag aaccctgcag 420

aacaagcgga acaagaacat caagcgcgtg aaccagaaga agggcaacat caagagaaag 480

agcatcctgc tgcacctcat cgtgctgaac agccccgaga gcaacctgca ggctcatcct 540

caagttccag cactggctcc tcaggcttgg tggcctgcta gaagaggccc tctgacaagc 600

gctccatctg ctcagcagag cctgaccttc ctgcctagca gatttccttg gggccctcct 660

ccactgcact ggaactctgc ctacctgggc agaacactgc tcgtgacctg catcctgtac 720

cacagatgga tcgtgaccag catgtgccag agccagagat ggctgaccag caccacctct 780

agaagcagcg ccctgatgtg gacccagtgg tctagcgtga caagcctgga atggacagcc 840

caagagggg gctgtagcag ctggctgatg aagcagacct ggatgaagtc ttggagcctg 900

cgggacccca gctacagatc catcctggaa tacgtgtcca cccagagtgct gtggatgccc 960

acatctacag tgggagccct gagagccaca gctcctgcaa cagctgctcc acaggctcag 1020

acccctcatt ggagagccgc cacaacaagc agcatgagca gcagccctag agccgagcct 1080

acaagctcta gctcttgggc cacctgttgg cccagatgtt gcagctccag ctgttactgg 1140

tccctgtcct cttggcctcc tgctggtgct gccgaggcca caaataccag gaccagaagc 1200

agagagtccc agcggggcag aatgctgact tggagaagca gcctgtggct gcagggcacc 1260

agatgtttta caggcgtgcg gacctccagc agacaccctt cttggccttg gaccagatgc 1320

ctgagaatgc ggcctcctag aagcaacagc cggaaaaagc tgctcgagga catgaagcaa 1380

aatggcttca cccggtacaa gaaacggggc gtgatagcct ggcggagagc cactttcctg 1440

ctggcccttt gggagtgcag tctgccccaa gctagactgt gcctgatcgt gtctaggacc 1500

ctgctgctgg tccagagcag atgcagcctg atggaacaga tccctcacct gaagtcctgg 1560

tggtgtacaa gaggcggcgg aaagcggaag aaaaagatgt gcaaggtgtc cggcggctgc 1620

accgcctgtg ttgaaagatg tccttggaag ggcatctgct ggaagtggcc tgtgacagtg 1680

gccgctctga tcctgattca gcacggcggc atcttcacct tccggttctg ggtcgagaag 1740

ctgtcctgga acatctgcaa gaatctgtcc ctgtacctgg acaagttcgt gcagcggctg 1800

ctgtacgtgt acatcatcga gacaaacgtg ttcgtcctga acctgttcca cgtgccagtg 1860

aatcagatct tcatgatggc cttctacctg aagtacaccg agaaggcctg ggtcatggaa 1920

ctggaatggc tcattcagca gcagtgctac cagttgctgc tggctcatat gtacggcttc 1980

agcacctacg gcacccagaa aggcgagtgc gtgtgtctgc agtgtctgag cggcagctcc 2040

ctgttcatcc tgaagcagcc actggacctg aagatcagca agaccttcat gagcagatcc 2100

cacctggatt tcagccccta ttggatgaag aaagtgaagg cctctccaca gatcatgcag 2160

2220

aagaacctgc ctctggaaaa cacacagatc cccaggacaa gccctctgtg gaagatcaca 2280

atggtgctga cataccccaa ctgcgtggtg catgtgctga tgttcgtgct gttcaaggcc 2340

gaccagagcg agtctagcct gagcagcgga atctaccctc ctagcacctg tagcctgcct 2400

tgccggggca ttagaatgtg cgccctgtct caccctagct tctacatcag cagcaccgtg 2460

tgctttctga gcaagcactg catgaccgcc tgcaccagag agaagatcat caaccccacc 2520

2580

gataatctgg gcctgctgta ctcctttcct ccttggctgc ccatgagatc cctgggcgaa 2640

ccttggagag ctggctttca gtggggagag tccccttggc aagtgatggc tagcctgacc 2700

ggcaacagcg tgatcgtgca caagatcgtg ctggtgtttc tgaagtctct gagcagctac 2760

aactgcgagc ggcggatctc tggcatccag aagagagcca tcgagttcgc cgtcctgaag 2820

ctgctgaaca tggacctggc tagccctcag attatgcaga gaaccagcgc cgaccggttc 2880

cggatgaagc ggagaatgca gaacctggat aacctggggcg tgccctggcc attgcttcca 2940

ggtgctgctc ctccacatag actgctgggc ccctctagat ggattctgtt tcatgtgcgg 3000

3060

caggtgcagc tggccattca ggtcctgctc gtggacaagc acaagtactt tctgttcggc 3120

ccctatccta tcagcgaggg actgaccatg accaccggcc ggaacatcaa cagcaactcc 3180

ctgatgacca actgcctgtt cagccccatc ttcctgtgcc accacgccat catgcaccgg 3240

aagatgacaa gcccacactt cagactgttc tccagcaaga tcccacatcc tcaggtgcca 3300

agtgtggtgg ccctgtgcaa gttcctggcc aatggcctgt ggttcaacct gatctttcag 3360

aaaaagggcc acatgatctt tagccccaag ttcttcctga cctttagcca gcggaccctg 3420

gaatccgccg gactgtttcc atggctgcac acccagtgcc ggaacaaaaa gaccaagctg 3480

tggttttccc tgatcaacat ccaccaccgc aagaatcctc tgctgcctat gagaatgagc 3540

agaacacctc ctcggagagt gtgcggatct ccacctctgc atctgtgcca acctctgaga 3600

ggcggcaaga agagaagcaa gttcgccttc tggaagcctc ctagccaggg catcagctct 3660

tgtctgatga caggctgttg gggctcctgt ggcagagctt gttcttgcgg aagaggctgc 3720

ggtacaccca gaggcgactg gaaaagacac ccacctcgca gagagctgag cctggaacca 3780

tggattcctt atctgcacca gcaaaagacc cagcggaagc ggatgaagac cggcgccatt 3840

caagagaagg gcgctatcca agaaaaaggc gcaatgacca agcggatctg cgtggtcaat 3900

ttcgaggcct gcagagagat ggaaagcctg agcgcccctg agaagattac cctgttcgaa 3960

gcccacatgg cccggtgcac ctccatcaat gtgctgtgtg tccgggccag cctgattgag 4020

aagctgatga aagaaaagcg caagatgaag cgcaaccagg tggcatcccc tcaaatcatg 4080

caacggctgt tcatcaaggg ctaccagaac aaccacttca cgttccacct ccaaaacttt 4140

gccttcttca gctccttcgg caacaacaag tatatcaaca ttctcacccg ggccttcatc 4200

cacacaccta gaagccctgg ctgttccgtg tacgccaata tgtgctggcg gaagatgcag 4260

tccctgaatc accccagcag actgaccaac tgtcaccccg cttggctgag ccccattagt 4320

gcttgtccta ggctgctgcc ctatcctttt cacgtgctgg cactggaagt gacctttcca 4380

cctggcacag ccatgcctca gatgcaattg caggcactgg ctatgtggga gaaaggctgg 4440

gacaagcaag agaagaaatc ccgcaagctg atgtcctcct gcctggaaaa aaaggctacc 4500

tggatgcgga gaacccctgg cacaagcctg cagtgttacc tgaacacaca cccctgcacc 4560

atgctgctgc ctcagggaat cccatctctg aacgcccagt ggatgttcct gcggatcggc 4620

tacccctacg atgtccctga ctatgcgtcc taaaggcgcg cctagcggcc gc 4672

<210> 1205

<211> 4687

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 3C cloning cassette

<400> 1205

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgacctatt 120

cctccacacc tgagacaccc tctgctgctg aagaacaaga agcggagaaa gctgcggccc 180

aagagcagaa agaagggcca caccaagaac cggcggaagg ccaagagaaa gccccagatc 240

agaaacagcc gcagagccac acctaccagc aagggcagcc tgagaagaaa gtacctgaga 300

gtgggagccg ccgctgctag ctgtcaatct cctccatctc ctagagagac aacccctagc 360

agcgacgcca ccagaagaac cagacctatc gccgctcaga gcaagaaaac catgacagcc 420

gccggactga cccctagcca gaagaaagat cctgccggca agctgaagaa acaggtgtcc 480

gtgtacagcg gcaacctgct gagcttctct cggagaggca tgaagtctag cgtgcgcctg 540

aagaactgca ccaacctgag cgtgcccatg atgctgacca tcctgatctg gaagcgggtg 600

ttcatcctgc tgctgtccga caagaaatct atccctcacg tgcccaaggc caccgccgct 660

cattctgtga cagtgaagct gcagagccgg cggaacagac ggtacagaaa gatctatggc 720

gagctgaaga tgtccctgca ggccagcaat ggcatggaag cctgtggcac acaccccaca 780

cctagactgt ccgtgtccgt gatcaccatg aagctgtggg agaagcactt caagggcctg 840

aactggaacc tggtcgtgtg gattctgaac ctgaagatga tgctcaagaa gaagtatctg 900

gtccagcgcg agtgggagaa acggaagcag attcttggcg gcggaggcaa gtacaaagag 960

tacttcctga agagaatcct catccgcaag gccatgacag tgctggccgg cgataagaaa 1020

ggcctgggca gattcatgag atgcgtgcag agcgagacaa aggccgtcag tctgcagctg 1080

cctctgggca gaatggacct ggatggaatg ctgaccgtga tcggccaggg actcgtgggc 1140

atgacaccta gagatctggg aacagtggct ggcgccaccg cacagattca gaggcctatt 1200

caggtgcaga caggcggcag actgagaggc cctagaagaa aggtgctgat ggtgctgtgg 1260

ctgcccgcca atcagagcct gcagaaaagc caggtgttcc agtgggtgct gagaaccgag 1320

ctgcccctga cctttagcac cctgctgtgg gacagcagac acgacctgcc tagaatgctg 1380

cccatcagca gccctctgca cagaccccag atgcctttct ggacctgcac ctttcctgcc 1440

accacatggg gcacatggga gacaccttct acatggggct ggcggacctt tgccagacag 1500

ccttctagac ccaccggcag aacccctacc accagactga tggctccagt gggcagcgtg 1560

1620

ctgcatcctc agtgggcccc tcattctgct ggactttctc caggcgcttg cagacctgcc 1680

tgtacctgca tcagcatgac cacaatcagc agggccacct ggcgcagaaa cctgcagaga 1740

aacctgaaag tgctgcggct gagaatcccc agcctgcgga agtacagaca gcagaacacc 1800

cggtccacca acctcgagaa gaacccatct ctgaagaagc agagccagag gcggggcaag 1860

ctgtctctga ccacactgag cttcgtgctg atcgaggaac tgctgaaaaa gagcagccag 1920

ctgcggagaa gctggcggaa cgtgtccaga atcagcaaca tccggaagct gatttggacc 1980

aagcagcgga gcttcctgtc tctgttcccc aagctgagag ccaccgagca gatgaccaat 2040

gtgggctgta gactggattt tcaccccaga agcagctccg gcgacagagt gttcctggtc 2100

aagatccaga gcagcccatt cctggccaga tcctggaata ccatgcagtg gaacaacaag 2160

aaaatggcca tgactgtgat cgctggcgcc caccactgtc agtacaagct gacctacacc 2220

aaagtgtgga agatgaattg gaagaaaatg atgatcggca gccagcacct cctgttctcc 2280

ccactggata gctctccact ccagctgctg ctgcaacccac acggcaagag atgcaacccc 2340

tgttgtcggc tgacatggat gagccacctg aatcggaagc tgcacctcac caggtcctgc 2400

ctgatcgacc tgaccatgat gatgagccag aaactgtgga agaatcagcg gaagcgcatc 2460

cctctgcctt ctccaagaca gcatctgctg cctcagtatc ctctgcaccc tcctccacct 2520

tgtctgctgc ttctccacca tagacaccac ctggcattcc tcgagatggc ctgtagctct 2580

cagcacaccc acagcatcaa gatctggatc agcttcagcc acgagacaat gagcctgtcc 2640

aacatcctga tgaagttccg gctgaccacc accacaagca gccccagaag aagccctgct 2700

ctgcgagcta gaggcggcac aacaatcggc gaagtgacca gcagcctgtt ctttagccac 2760

tgcctgtgcg tggcccctag atgtcctaga tctgccgtgg acatcagccc cggacctaca 2820

tctccttctc tggcctcttg tgcctctgtg gccattggaa agctggcact gcttggcgcc 2880

tctgctcctt gtggtgctgc tggcgctaga gaggccggaa gatttgctgt gctgggcacc 2940

atggtcatga agaacatgga acacagactg accatggaca gcgccctgag cctgacaaag 3000

agagaggggcc ctgaaacagg ccccgtgtac tacgtgtgca accggatgaa tgtgtccagc 3060

3120 gtgctgaagc ctttcctgtt caccgtgcct

cgcaacagaa tgaatgtctc ttccgtgctg aaaccctgtc tgatccccgt gccaatctgc 3180

aagaagaaac tgctgctcaa gctgctgagg ggcgcctcca gctctaccag cagagatagc 3240

agaagctttt tcaccagcgc tcaccccagc agaccctgca ggatgagact gtaccagatg 3300

ctgcagaagc agtgctgcta caacctcgtg atcaagcacc ctctgatctt ccggatcaac 3360

ctgcggagac agggcaagac caaagacggc gttgtgcaga gcctgggctt cctgaaccag 3420

cagatcatcc tccagaaaga gttcttcatc tgcctgctga tgttcatcaa gcggtggaag 3480

ccagccgcca ccaagaaggg caagaacagc ccctgctgcc agaaaaaact gcgggtgcag 3540

aaccaggggcc atctgctgat gattctgctg cacaacagag gcccacctct gcataccaga 3600

3660

ctgtgccctg ccttcagcgg caggattctg caccagccag aaatggccgc catgagactg 3720

atgcccaagt tcctgaactc caccaacagc tggtggacag tgcacaagaa gagaggcctg 3780

ttccaccaga tgaagtgtct gaccgccgtg gactgtctgc aggccttctg ggatatgagc 3840

gagaagaaac gcaagctgcc tatgattcgc aaaatccaga agcggcggag aaccgccaac 3900

3960

ggcgccaaga aaatcacctt cctgtgcctg agagtcagat gcggaggctg gctgtgtggc 4020

tggacaccaa gaggatggct gaagaagtcc accaagtcca gactgtgctt cttctacctg 4080

ctgtactacc tgtttttcct gaccgtgtgc ggcttcaccc ctccaccact tattctgtgg 4140

cctcaggcag cccctggcga gaagctgaag cactgtttta aagcctgggg cgtcgaccct 4200

cctggcagcg tttggattct gccctgcttc ctgattcacc tgagaaaggt ggacacccac 4260

cacctccagg tggtcaccaa aacagtgacc gccacaacag ccgacggcca gaggatgaga 4320

aggctgaagt gcaaggcctg ccggctgaga tgggctagac ctgaacctgc agctcaggcc 4380

cctcctaaga gccactttcc acctcatcct agcggcccct ccagaaccag ctctctgcaa 4440

ttgagaacct acatggaaat gcactacggc catcaggccc cacaggtggc aatgctgcct 4500

aaactgctgt gtcggcagat gctccccact ctgtctaccc tgcagcagag acatcctcag 4560

ggcccatgtc ctcctccagt gacacctctg agagtgcagt ctgtgctgct gctcggagtg 4620

cctcaaacag ctggctaccc ctacgatgtc cctgactatg cgtcctaaag gcgcgcctag 4680

cggccgc 4687

<210> 1206

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 4C cloning cassette

<400> 1206

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgagacagc 120

180

accgacaacc tggacctgca gcaccagcgc agacgcaaaa agaagaagga caaggtcgag 240

atccggaatc ggagccctaa ggccaatagc accgagtgtc agggcgagca cagcagagag 300

cagaacctgc tgaacctggt gcaattgaac cctcacagcc ctcaccaccg gaaggacgag 360

gaagatcacc agaagagaca ccaccaccac aatcaaaaga agatgtccgt caacgagggc 420

aacaaccact ggcctagagt ggaaatgcct accggctggc tgctcgtggg ctacaacacc 480

agaaacacag cccagaatcc tcggctgcag cagcacctga aacactggca gcagtgcacc 540

agagccagat ggtgtcaaga gccctgtcag accctgacct tcttccactt cttcttcttt 600

ttcaaaacgg tgtccctgtg ctgccccggc tggtctaaag gacaacctcc tccacctggc 660

ctgaagcctt ctagcctggc ttctcaggtg gccagaacca caggcgtgta ccagcacgtg 720

tggctgatct gcttcgtgtt tttcgtgtgc gtggaaaccg gcttctgtca cgtggcccaa 780

gtgggactcg agagactcgt tagcctgagc ggcatcaccg acgtgaacca ttgggcccag 840

cctaagccat ctcctagcaa aggcaccggc atgaacagac ccatcggcct gagagttggc 900

aaaggcgtgt gggctagact gtggctgaag aactggaagc ggttcaagct caaaaagaaa 960

tacacaggca tggaaatcct gctgtggatt ctgctgaaga tggaaattca gatcttcaag 1020

atttgcctgg gcgagatgca gctgtgcctc aagaagaaag tcaacgcctg cagcaacgcc 1080

aagaaaatgc ccttccagtg ccggtgcaga cgcgagtaca gagtgaccat gattcagaag 1140

aagaaacaga agtccccgct gttcttccac ggcgacctgt gcagaccttc tccttgcctg 1200

tcttggagca gcaggatcgt ggctcctgga cacgccctga gctgtcggac tcgtagaaga 1260

tggtccagca agatggccaa gaagtcccag cagcctctgt gtagaggcgg cccttctgcc 1320

agcgatagat gctgtagctg tagccccagc ggctttagcg ctggaagagg cagatgtcct 1380

gtgcagggat gtctgaggcc aacacagagtt cagctgctga gaagatgggg ccctggatct 1440

cctgctggcc aggactgtc taagggcttc caactgctgc gttggtgggg accaggatct 1500

ccagctcctg agcctagaaa gggcccattt ccaccacctg atcctccatg gcctgtgacc 1560

gctgtgacag tgatggctgg ctctgtgcct agcgctcagt ctgtggatgc tctggaatcc 1620

cctggacctc tggctctcga aggccctagc agccctagaa acctgctctg gcgggaaatg 1680

agcatcttcc tgcctggcat cttcaacctg cacctgtgct actaccacag ccagagcaac 1740

cggaacaaga gccggcagat ggaatccctg ggcatgaagc tgcagatcac acacccattc 1800

gccagccagc cactgggcca agagatgccc aacaacaccc agatcagact gctgaagccc 1860

ggccaggcct ttggcagctt ttccgccgat ttttcagccc ctcagcaggc ccctctgaga 1920

ctgtggtctt ggtgcggcac aagccagacc tacttcggct ctatcgaagc cctgacagcc 1980

ctgttcacct gtagaaccgc cagcatcagc gtgaacatca gcaccctgat gagcatcctg 2040

gaagccatcc tggccatgac actgaccctg aatggcagcc ctctgtggga ctgcagcaca 2100

acaacaggca cacaccacgg ctgcctgtgg gagatccctt ctgttaccct gctggctacc 2160

gccaaaaaga gccctaccag cttctgctgt cagcggcctc ctctgcagcc agtggaagag 2220

aagaagaacg gccggatcag cagcgtgcca ccttctccac ctcttgctct gggccctaga 2280

atgcagctct gcacccagct ggcccggttc ttccctatta cacctcctgt gtggcacatc 2340

ctgggacctc agagacacac ccctctgctg acccctgaca ccagatggat cttctccaac 2400

cagctgcaga ccgtgcagat gctgtgtctg cctccactga agcagggcga gagaatcatc 2460

tggcctctcg gcggctttca gatccccaac ttcttttgga gcctcggcat cggacacact 2520

ggcgcctggt ggcatactgg aaggcggaga agagaatggg gcggcagcac cgtgtctaga 2580

agccctgt ggatcagctc catgatctac ctgagaggca tggccttcgc ctccgacagc 2640

agcatcagaa ccaccagagt gtcctggtgt cggagatgtg gcgccaaaag cgccgcagcc 2700

agcagcgaca agaagaagaa aagcaccgaa aagaccaaga acaagatcat ctgcaagggc 2760

cacagaatca cccaggtggt gctgagactg ggcaacctgc ctccattctt cagaggcagc 2820

gaggtgccct ttcctagacc ttgccggacc aagtgccaga agaaggttcc aagcgcctct 2880

ccttggggac tgcacccaag aacagcctcc atcacactgc ctaccaccag cctgaagggc 2940

agcagaacaa gctgctgccc cagatctcac cccagactgc ccagaacaaa gtggcctctg 3000

3060

cagatgagaa gtctggaagc tcactgccct ctgcagacat tccacctcca ccactgcaga 3120

acccagccta gctcttgtcc tccagcctgg attctgtacc aaggacagtg gagcggagcc 3180

gtcgtgggac ttagacctgt ggctcaccat ctgccaagac ctatccaggg gctgcactgt 3240

cctcaggctc ctcatcaacc agctctgtcc tggggaaagt ggcccgtggg aatgacagga 3300

cccgccagat gttgccggtg gcctagaaga atgtgtagct ctctggccct gccaaccggc 3360

ctgacatctc tgatcctgcc tagcagtgat ctggccgtgc tgatctctag ctccaccagc 3420

cactttctga tgcggagccc agtgctgcca agcagcagac tgacatgtgc ctctccacag 3480

ctgcctcgga tgtggacatg gtctagctgg ctgaaagagt ggggaggaca ggcccacaga 3540

cagcggaact gtagagtgaa cgccgttgag gaactgcaca gcaggcggat gtctgtgcgg 3600

atcgtgaaga attgggtgtc ctgctgttac gtgcggctga gtgcagtggg actgtctact 3660

tggagcgccc tggattccct gatcctgtcc ttccagagag tgaaaaccag ccggatactg 3720

aacctccagg tgctgaagaa gatcctgcgg agctttatga agctgtacca ctctctcgtg 3780

atgtgcctgc ggctgcggac caagctggaa aaagctctga gcgctctgtt tatctggccc 3840

cagcacagct acaaaggcca ggcattcggga agcctgtgcc agctggtgtc tgccgatttc 3900

agcgcccctc aagtgaccag actgcggagc ctgaaccatc tgctgattcg gtggatcttt 3960

agcaatcagc tgacagtcca aatgctctgc ctgccgatca tcaagaaaat gaagaaacgg 4020

atcatggtgc acgtcttgaa gaagaatcgg aagcagatta tctgtatcaa gaatatgttc 4080

ctgcagcgga agggattcct gaacctgagc cacgtggaaa agttcagaga gagcaccctg 4140

ggcaatttct ggatgaagct cctgcagctg ccaccagctt gcacctctaa ggcctggaca 4200

cccagaaagc ttgtggggag agccgtgcgg agaaagggcg tcgtgatcaa cctgaagaga 4260

ctgcagaaat tccgggctag cgtgtccttc tggactctgc tgcctacaac aggcgtgcgg 4320

acaagaggct ctccactgag actgactctg tggcctccaa gaagccagaa aagccagcac 4380

cactgcgccc tgagaaagat cgagtgggcc acaaagctgt ccctcggcgg caaagcagga 4440

ccacaggctc aagagaaggc caacatcctg aacagcctgc cttcctctat ggaaatcgcc 4500

gtgctgcaag aaatgctgaa ccagaagtgt ctggcccctt tcgagcttaa gaagaacctc 4560

ctgctgccaa cctacaccc ccacatctgt cggcacaccc ttaaaaagaa gccgagac 4620

atctgcagaa tcagcggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1207

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 1D cloning cassette

<400> 1207

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgagccgac 120

aagaaaaacg tgcggagcgc tagttctcct ggacatcctc ttggccccag aagaatgacc 180

agacggacca tgaccaccac caaaatgatg atgatgcggc ggaccaagcg gcggagaaga 240

aaaagacgcc ggatgaccat gatgaccaga agaaccctgc tgaccaagaa aacctgtctg 300

360

cctatccaga cacctcctgg aacaagcctg gccatctgggg gcagaagaag caagatcgag 420

gcctctccta tcggcagcat cacccctctg agcatcagca gacctaccag cagagccaga 480

cagagctgtt gcctgcctgg actgacacac cctgctcatc agcctctggg ctctatggtt 540

ggagctggaa caggcgggagg acctgcttgt gtgcctgtgg ccgaagcttc tacaggcgct 600

tggacagctg aaggcgctgc cggcacaaga tgtagagcca gcagagcccc ttgcagacag 660

ctgcctgctt ctagccctac caagtgcgag atctgcgtga agagaagaag gcggcgcaag 720

cggagcagcc agtctagatc tcccgtgcct aagaaacgcg agcggatgat gctccctgtg 780

cgggccagaa cagctagatc tgctctgcca caaggcaggg ctcctctgga aggaatgctt 840

ctgcctagag cactgcccag cctgctgagc agaatgcctg ccagaagaga gagcagattc 900

tggctggccc tgcaggccct tggactcaga cctcctcatc tgcctagctg tcaccctctg 960

cctcaactgc ttggacctcc tccacctcat caggcccctg gcgagaaaaa cgacctgcag 1020

ctgttcgtga tgagcgacac cacctacaag atctactgga ccgtgatcct gctgaacccc 1080

tgcggcaacc tgcacctgaa aaccaccagc ctgaacgcca agaaaatgga agatatgatc 1140

tgggagagca accagcagct gaagtggacc cctcacatcc tgaagtccgc ctctatcgcc 1200

aagaagcgga tcaagctgcg gcggaacagc accagacggg aaatgccttc tcaggccaca 1260

ccttctccac ctggaccttg gagagccgct ctgggcaaag aagtgaagcg gagccagagc 1320

1380

atgggcggca accggtctat ctgcctgacc atcgtgttct ttctggcccc ttacttcccc 1440

atgcctagca gctggtctag cagcggcaga accagactgc ctctgaggcc acagagaggc 1500

cctagacctc tgcatagatg gctgcagcag accaccggct gctctcagag aagaggcgac 1560

tgcccaagaa ccatggccgg accatctgct tctctgcacc ctgcatctgg cagagcctcc 1620

agaaagacct ggcagagaag gctgagagcc tgtagagcag gcggacctgc tggatgggct 1680

agacaggcca gaaaacgcaa gagagagaag cggtcccaga agtccagaca gaaccatctg 1740

cagctgaaaa gctgcagacg gaagatcagc aactggtcta agctgcggag cccctggatc 1800

ttcgaggcca aaaagagcat gaagagatcc cagcggcctc ctcggagcat cctgagcctg 1860

ttcaagaaga agaagcccct gacattcagc cccagcgtgt ccaagaagag gctgcctaag 1920

ctgaagggca agaaacgccg gaaagaaatc cagcgggaagg tggtcaagaa ccccagccag 1980

cctgtgccac tgagaaatga cggcatcacc aacggcaacg acagcacccg gtcttgcagc 2040

atcagcctga agaaaagcct ggaaaagatg cccctgatgc ggagcctgct gtactccaga 2100

atgtgcctgg tgcagatccc cacacacacc tgggagcccg actactttgt gtccctgcct 2160

atgatgttca gctgggccac acagagcttt ccagtgggcg ccgtgtttct gaccagacct 2220

gccattcctt gctgtagctg taccagccac tccaatcctt ggagccagtc cacactggcc 2280

atccggttta tctctgcccc tcagccaagc agcagcaccg ttacagctgc tcctagcggc 2340

cagtattttg ccctgcggag tatgcccaag agccggaacc aggtgctgtt tcggagaaat 2400

ctgaccccaa gccagctgcg gacactggcc cctccatata tgctgctgcc tcagtctctg 2460

tccgagcc tgtctcggaa gcagatccca tctcagtcca tgctcgtgcg gaccaggctg 2520

acactgacag gcactagaag ctccggcacc agagcctctc catctgccgt gtgtctgtgc 2580

tgctggacat gccccagcag aagctttcct gccgcctctc caagcggact gagagcacct 2640

gttggagcac aggctcctcc agaagtgaca ctgagagcca gctgtgccag ctccgccagc 2700

2760

accatcttca tctaccccag gctgaaaacc ttcggcgtgc cactgggcag cattctgtgt 2820

ctggctggaa gcctgagcac gatgatgatc attagcacca ccagacaaga gtgcggcaga 2880

cgggccagcg tgccctgtag acctatgatc ggaagcgcca gacctggacc atggcggaca 2940

agtgctatgc cttctgccat gggagtcgcc ctgcctacca gctgtgaaag cggaagatct 3000

3060

ccactggctc ccctgcgctgc ttgtccttgt tctcctgctc ctacactgtg ccctgctcac 3120

cctgctagag caccaacagc cgtgcctgcc tttacacccc tgtctgctca tcctgtgcca 3180

gtgctgtctg gatgtcacct ggctgtgcgg acctccatgc ttaccttgct gccaatgatg 3240

accctgaagg ccctggtcaa gacatggacc cagttcagaa actaccccag acggcaccct 3300

cacagaagcc caccaacaag ctgtcctcca ctgctcgagg cctttccact gccaagagcc 3360

agcctgagag ctaccgtgcc tagacactct agcgtgctgg ccacaggact gcatctgcat 3420

aggcagatta gcctgcctct gcggggcttc ctgctgcatc aaccactgcc aggacaacac 3480

ctgtggctgt cccacagact gagactgcgg ctgcctagaa caaccgagat ccagctgacc 3540

atgaacgact ccaagcacaa gctggaaagc cccgctctga aacaggtgtc cccagctagt 3600

cctcctacac agcagcctca gacaccccag gattctagac aggtgctggc cgtgcctatg 3660

agcctggcct gtagcatcac cgtgtccttc gtgggccaga tctctaccct ggtgctggac 3720

gtgtcctcca tcagccactg gaacggcatc tgggaagtga tgcccagaac actggaaggc 3780

tgcatctccg tgaaggacca ccggacatcc cagaaccaca gcaagtgcat caccgagtgt 3840

gtgcggacag ccctgaacac caagtgtgaa atttgtgtga agaacaccgc catcctggct 3900

atcaccctga tcaccccaca ggaccctcac ctggaccctg tgtgtcatcc ccatagaagg 3960

cccggctgga agggacacca cagagtggtg tttagccacc atccttccgg cagcaccaag 4020

4080

gcaagcagag ccggccagtt tcctgtgtgg cggagaacat ctagaggcat gtccctgcag 4140

tggaagtccg gcaatgcctg cacacctctg agatgtccca gcagatctcc agccaccttc 4200

ctgactgccc tgaacggctg gcatagatct ggactgaggg cccagaccgc cttctttctg 4260

ctggacatca tcctgcggag agcccggcct acaagctgca gcacatgtag aatgtggtgg 4320

cggctgccct gcaaagtgac cgaaagctcc ctggccaaga aaaccctgac caatcagcct 4380

agcttgcccg gaccttgtgc tagcctgctg tctacactga gccagccacc acctcaggct 4440

cctagccaag tttggacagc cgctacactg cggtgtcctg ctgtgcctgc tgctgcatgt 4500

ccacctctga agctggctcc actgcaagtg accacaacac tgaagctgat cctggtcatg 4560

ctggaaatcg tgaccgagaa caaagttcgg cagaacttca agcggaactc cagcttcttc 4620

tacaccagcg acagaggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1208

<211> 4672

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 2D cloning cassette

<400> 1208

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgacggaag 120

atgcagagcc tgaatcaccc cagcagactg accaacaatc ctcggagaaa gacctggaag 180

atgcggaaga agtacgccca gaagcctagc cagaagaacc acctgtaccg gcagatcaac 240

cggcgcaagc tgcagagaaa gaagtccaga agccacctgg acttcagccc ctactggatg 300

aagaaagtga agcgggccag aaaccggaac atgaagaaaa ccatgagcca cctgatcgcc 360

catcaggccc tgaagatgtc tggcctgaga ctgtttcacc ccagactgct gaccagaaag 420

aagcccggca agcagaaaaa gggccacatg atcttcagcc ggcggaagtg gcagatccgg 480

cacgtgctga agaatctgat ccccagcctg aacgcccagt ggatgttcct gagaatcgct 540

agcccacaga tcatgcagag aaccagcgcc gaccggttcc ggatgaagcg gagaatgcag 600

aacctggaca atctgggcgt gccctggcct ctgttgcctg gattgctgct ggcccatatg 660

tacggcttca gcacctacgg cacccagaaa ggcgagtgcg tgtgtctgca gtgtctgagc 720

ggcagcagcc tgttcatcct gaagcagccc ctggacctga agatctccaa gaccttcatg 780

gccagccctc agattatgca gcggatgagc gccgtgaact tcgagacaat cctgcaggcc 840

catcctcagg tgccagctct tgctcctcaa gcttggtggc ccgctagaag aggccctctg 900

acatctgctc caagcgcaca gcagtccctg acacggacac tggaaagcgc cggactgttt 960

ccttggctgc acacccagtg ccggggccttc attcacacac ctagaagccc tggctgcagc 1020

gtgtacgcca acatgtgctg gcggtacaag aaacggggag tgatcgcctg gcggatctac 1080

cctcctagca catgcagcct gccttgcaga ggcctgatcc tgattcagca cggcggcatc 1140

ttcaccttcc ggttctggggt cgagaagctg agctggaaca tctgcaagaa cctgagcctg 1200

tacctggata agttcgtgca gcggctgctg tacgtgtaca tcatcgagac aaacgtgttc 1260

gtgctgaacc tgttccacgt gccagtgaac cagatcttca tgatggcctt ctacctgaag 1320

tacaccgaga aggcctgggt catggaactg gaatggctga tccagcagca gtgctaccag 1380

cggaagagaa tgaagaccgg cgccatccaa gagaagggcg ctattcaaga gaaaggggcc 1440

atgaccaagc ggatctgcgt ggtcaatttc gaggcctgca gagagatgga aagcctgagc 1500

gcccctgaga agatcaccct gtttgaagcc cacatggccc ggtgcaccag catcaatgtg 1560

ctgtgtgtca gggccagcct gatcgagaaa ctgatgaaag aaaagcgcaa gatgaagcgc 1620

aaccaggtgg catccctca aatcatgcaa cggctgttca tcaagggcta ccagaacaac 1680

cacttcacgt tccacctcca aaacttcgcc ttcttcagct ccttcggcaa caacaagtat 1740

atcaacatcc tgacctttct gccagcaga ttcccttggg gacctcctcc actgggagcc 1800

cttagagcta cagctcctgc tacagctgct ccccaggcac agacacctca ttggagagcc 1860

gccaccacaa gcagcatgag cagctctcca agagccgagc ctaccagctc tagctcttgg 1920

gccacttgct ggcctagatg ctgcagctcc agctgctatt ggagcctgtc tagctggcct 1980

cctgctggcg ctgccgaagc cacaaatacc agaaccagaa gccgcgagag ccagcgggga 2040

agaatgctga cttggagaag ctccctgtgg ctgcagggca ccagatgttt tacaggcgtg 2100

cggaccagct ccaggtgcag cctgatggaa cagatccctc acctgaagtc taacagccgg 2160

aagaaactgc tcgaggacat gaagcagaac ggcttcacca gacggcggaa cagcagaaag 2220

cagagccaag tgcagctggc catccaggtg ctgctggtgg acaagcacaa gtactttctg 2280

ttcggcagag ccacatttct gctggctctg tgggagtgta gcctgcctca ggctagactg 2340

tgcctgatcg tgtctagaac cctgctgctc gtgcagtcta gacacccaag ctggccttgg 2400

accagatgcc tgagaatgcg gcctccacgc agccactgga actctgccta cctggggaga 2460

acactgctcg tgacctgcat cctgtaccac agatggatcg tgaccagcat gtgccagagc 2520

2580

acaagaggtg gcggcaagcg gaagaaaaag atgtgcaagg tgtccggcgg ctgcaccgcc 2640

tgtgttgaaa gatgtccttg gaagggcatc tgttggaagt ggcctgtgac agtggccgcc 2700

atgagcagaa cacctccaag acgcgtgtgc ggccacagag agggcagcga gtctgataat 2760

ctgggcctgc tgtactcctt tccacctgtg ctgttcaagg ccgaccagag cgaaagctct 2820

ctgagcagcg gcaacagcgt gatcgtgcac aagatcgtgc tggtgttcct gaagtccctg 2880

agcagctaca actgcgagag aagaatcagc ggcatccaga agagagccat cgagttcgcc 2940

gtcctgaagc tgctgaacat ggacctcggc aagggactga cacaccccgt gcctctgaag 3000

aggaccctgc agaacaagcg gaacaagaac atcaagcggg tcaaccagaa gaagggcaac 3060

atcaagagga agtccatcct gctgcatctg attgtgctga acagccccga gagcaaccgg 3120

gaaaagatca tcaaccccac catcagctgc ccctttcaga gccccacaaa gagactggga 3180

agccctcctc tgcacctgtg tcagcctctg aggccctatc ctatcagcga gggcctgacc 3240

atgaccaccg gcagaaacat caacagcaac tccctgatga ccaactgcct gttcagcccc 3300

atcttcctgt gccaccacgc catcatgcac cggaagatga caagccctca cttcagactg 3360

ttctccagca agatcccaca tccacaggtc ccaagtgtgg tggccctgtg caagtttaca 3420

cacccctgca ccatgctgct gccccaaggc gctttttgga agcctcctag cccagggcatc 3480

tccagctgtc tgatgacagg ctgttggcag tggtccagcg tgacaagcct ggaatggaca 3540

gcccaagaga gaggctgttc ctcctggctg atgaagcaaa catggatgaa gtcttggtcc 3600

ctgcgggacc ccagctacag atccatcctg gaatacgtgt ccacacggggt gctgtggatg 3660

cccacctcta ccgtgcctaa gttctttctg accttcagcc agatccggat gtgcgccctg 3720

tctcacccta gcttctacat cagctccacc gtgtgctttc tgagcaagca ctgcatgacc 3780

3840

aacctgatct tccggaacaa aaagaccaag ctgtggttct ccctgattaa catccaccac 3900

cgcaagaacc cactgctgcc catgcggctg gaaaaaaagg ccacttggat gcggcggacc 3960

gagctgtctc tggaaccttg gattccttac ctgcatcagc agaaaaccca gtggctcccc 4020

atgagaagcc tgggcgagcc ttggcgagct ggatttcagt ggggagagtc tccctggcaa 4080

gtgatggcct ctctgacagg cagacgcaag aaaagagagg atcactgcgg actgcaagag 4140

aaaaaaagcc ggaagctgat gtccagctgc gccgctcctc cacatagact gctgggacct 4200

agcagatggg gaagctgtgg gagagcctgt tcttgtggca gaggctgtgg aacccctaga 4260

ggcgactgga agagacaccc acctcggcgg attctgtttc acgtgcggaa catcgtgtac 4320

ggaatccaag tgatgctgtg ctgtcacccc gcctggctgt ctccaatcag cgcttgtcct 4380

cgcctgctgc cttatccatt ccacgttctg gccctggaag tgacattccc tccaggcact 4440

gccatgcctc agatgcaatt gcaggcactg gcaatgtggg agaaaggctg ggacaagcct 4500

ctggaaaaca cacagatccc caggacaagc ccactgtgga agatcacaat ggtgctgaca 4560

taccccaact gcgtggtgca cgtcctgatg ttcggcggca agaagcggag caagttcggc 4620

tacccctacg atgtccctga ctatgcgtcc taaaggcgcg cctagcggcc gc 4672

<210> 1209

<211> 4687

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 3D cloning cassette

<400> 1209

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgacagaac 120

accagaagca ccaacctgga aaagaacccc agcctgaaga agcagagcca gcggagagga 180

aagctgagca gcagaaagaa gggccacacc aagaaccggc ggaaggccaa gagaaagccc 240

cagatcagcc agaagaagga ccctgccggc aagctgaaga aacacctgaa ccggaagctg 300

cacctgacca gatcctgcct gatcgacctg accatgatga tgagccagaa gctgtggaag 360

aaccagcgca agagaatccc tctgccttct ccacggcagc atctgctgcc tcagtatcca 420

ctgcatcctc cacctccatg cctgctgctg ctccaccaca gacaccatct ggcctttctc 480

gagatggcct gcagcagcca gcacacccac agcatcaaga tctggatcag cttcagccac 540

gaggacctgc ctagaatgct gcccatcagc agccctctgc acagacccca gatgcctttc 600

tggacctgca ccttttcctgc caccacatgg ggcacatggg agacaccttc tacatggggc 660

tggcggacat tcggccctgt gtactacgtg tgcaaccgga tgaacgtgtc cagcgtgctg 720

aagcctttcc tgttcaccgt gcctatcgag gacatgagag agtgcaccct gggcagaaac 780

agaatgaatg tgtcctctgt gctgaaaccc tgtctgatcc ccgtgccaat cctgaccaca 840

ctgagcttcg tgctgatcga ggaaagccgg cggaacagac ggtacagaaa gatctacggg 900

gagctgaaga tgtccctgca ggccctgcct ctgaccttta gcacactgct gtgggacagc 960

agacacacca ccaccacaag cagccccaga agatcccctg ctctgagagc tagaggcggc 1020

accacaatcg gcgaagtgac aagcagaggc gccagcagct ctaccagcag agacagcaga 1080

agctttttca ccagcgctca ccccagcaga ccctgcagaa tgagactgta ccagagcaac 1140

ggcatggaag cctgcggcac acaccctaca cctagactgt ctagaggccc tccactgcac 1200

accagaacca tgagcctgag caacatcctg atgaagttcc ggctgatgct gcagaagcag 1260

tgctgctaca acctcgtgat caagcaccct ctgatcttcc ggatcaacct gcggagacag 1320

ggcaagacca aagacggcgt tgtgcagagc ctgggcttcc tgaaccagca gatcatcctg 1380

cagaaagagt tcttcatctg cctgctgatg ttcatcaagc ggtggaagcc agccgccaca 1440

aagacacctc caccactgat tctgtggcct caggccgctc ctgtgcacaa gaagagaggc 1500

ctgttcagcc tgttcttctc ccactgcctg tgccaccaga tgaagtgcct gaccgccgtg 1560

gattgtctgc aggccttctg ggacatgatg attcgcaaga tccagaagcg gcggagaacc 1620

gccaaccggc tgaaaaagaa atacctggtg cagtgtacca acctgagcgt gcccatgatg 1680

ctgaccatcc tgatctggaa gcgggtgttc atcctcctgc tgagcgacaa gaagtgtctg 1740

agagtcagat gcggcggatg gctgtgtggc tggacaccta gaggcaagaa caagaagcgg 1800

agaaagctgc ggcccaagca cctgaagaaa accagcgcca aggtgtccgt gatcaccatg 1860

aagctctggg agaagcactt caagggcctg aactggaacc tggtcgtgtg gattctgaac 1920

ctgaagatga tgtggctcaa gaagtccacc aagagcagac agagcagccc attcctggct 1980

cggagctgga ataccatgca atggaacaac aagaaaatgg ccatgaccgt gatcgctggc 2040

gcccaccact gtcagtacaa gctgacctac accaaagtgt ggaagatgaa ttggaagaaa 2100

atgatgatcg gctcccagca cctcctgttt agcccactgg attcaagccc actgcagttg 2160

ctgctgcacc ctcacggcaa gagatgcaac ccttgctgca gactgacctg gatgagcaga 2220

gagtgggaaa agcggaagca gatcctcggc ggaggcggca agtacaaaga gtacttcctg 2280

aagcggattc tcatccggaa ggctatgaca gtgctggccg gcgataagaa aggcctggga 2340

agattcatga gatgcgtgca gagcgagaca aaggccgtgt ctctgcagct gcctctcggc 2400

cgaagaaagt ccggcgtgat gaagtgttgg gatctgtgcc ccagactgga ctttcacccc 2460

agatctagct ctggcggcga gaagctgaag cactgtttca aagcctgggg cctgtgcttc 2520

ttctacctgc tgtacttcga gaagaaacgg cccgagtggc tgagcgttga ccctcctgga 2580

tctgtctgga ttctgccctg catggacctg gacggaatgc tgacagtgat cggccaggga 2640

ctcgtgggca tgacccctag agatctggga acagtggctg gcgccaccgc acagattcag 2700

aggcctatcc aggtgcagac aggcggcaga ctgttcctga ttcacctgag aaaggtggac 2760

acccaccatc tgcaggtcgt gaccaagacc gtgacagcca caacagctga cggccagagg 2820

atgcggagac tgaagtgcaa ggcctgtcgg ctgagatggg ccagacctga acctgctgca 2880

caggctcctc caaagagcca ctttccacct catcctagcg gcccctccag aacctctagc 2940

ctgcagctca gacccattcc tccacacctg agacacccac tgctgctgaa gggaaagaac 3000

agcccctgct gccaaaagaa actgcgggtg cagaaccagg gccatctgct gatgatcctg 3060

ctgcataacg ccagacagcc cagcaggcct accggaagaa cccctaccac aagactgatg 3120

gcccctgtgg gcagcgtgat gtcttgtagc ctgctgaccc agccatctcc tgccagcgca 3180

tgtacaatgc caccactcct gcatccacag tgggccccac atagtgctgg actgtctcca 3240

ggcgcttgca gacctgcctg tacctgcatc agcatgacca ccatctccag agccacatgg 3300

3360

tctaaggccc aggtttccgt gtacagcggc aatctgctga gcttctccag acggggcatg 3420

aaagtagcg tgcggctgaa gaacagcatc cctcacgtgc caaaagccac cgccgctcac 3480

3540

ttcctggtta agatctgcaa gaagaagctg ctcctcaagc tgctgagagg ccccagacgg 3600

3660

cagtgggtgc tgcggaccga aacctacatg gaaatgcact acggccatca ggcccctcag 3720

gtggccatgc tgcctaaact gctgtgtaga cagatgctcc ccactctgtc cacactccag 3780

cagaggcatc ctcagggacc ttgtcctcct cctgtgacac ctctgagagt gcagtctgtg 3840

ctgctcctgg gagtgcctca gacagccgct tttagcggcc ggattctgca ccagccagaa 3900

atggccgcca tgagactgat gcccaagttc ctgaactcca ccaacagctg gtggacagtg 3960

gcccctagat gtcctagaag cgccgtggac attagccccg gacctacatc tcctagcctg 4020

gcctcttgtg cctctgtggc cattggaaaa ctggcactgc tgggcgcatc tgccccttgt 4080

ggcgcagctg gtgctagaga ggctggaaga tttgccgtgc tgggcaccat ggttatgaag 4140

ggtgctgccg cagcctcctg tcagtctcct ccatctccaa gagagacaac ccctagcagc 4200

gacgccacca gacggacaag acctattgcc gctcagagca aaaagaccat gactgccgcc 4260

ggactgaccc ctcggagaaa cctgcagaga aatctgaagg tcctgggcgc caagaagatc 4320

accttcctga gaaacagcag acgcgccaca cctacctcta agggcagcct gagaagaaag 4380

tacctgagag tgaacatccg caagctcatc tggaccaagc agcggagctt cctgtctctg 4440

ttccccaagc tgagagccac cgagcagatg accaatgtgg gatgcctgct caagaaaagc 4500

agccaattgc ggcggagctg gcgcaatgtg tcccggatca gcaacatgga acacaggctg 4560

accatggaca gcgccctgag cctgacaaag agagagggcc ctgagacaag cgagaagaag 4620

aggaagttgc ctggctaccc ctacgatgtc cctgactatg cgtcctaaag gcgcgcctag 4680

cggccgc 4687

<210> 1210

<211> 4690

<212> DNA

<213> Artificial sequence

<220>

<223> hTPA 4D cloning cassette

718

<400> 1210

gaattcggat cccgcgactt cgccgccatg gatgcaatga agagaggggct ctgctgtgtg 60

ctgctgctgt gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgaaacaga 120

aagaccagaa aggccgccag agagaagcac cgcaagaccg acgacaagaa gaagaagtcc 180

accgaaaaga ccaagaacgc caagaagatg cccttccagc agcggcctcc tctgcagcct 240

gttaacctgg atctgcagca ccagcgcaga cgcaaaaaga agaaagacaa ggtcgaaatc 300

360

aacctgctga atctggtgca gctgaaccct cacagccctc accacagaaa ggacgaagag 420

gaccaccaga agcggcacca ccaccacaat caaaagaaaa tgagcgtgcg ggctagcgtg 480

tccttctgga cacttttgcc aacaaccggc gtgcggacaa gaggctctcc actgagactg 540

acactgtggc ctccacgggt tccaccttct ccacctcttg ctctgggccc cagaatgcag 600

ctgtgtaccc agctcgcccg gttcttccct atcacacctc ctgtgtggca catcctggga 660

ccctcagagac acacacctca ggctcctctg aggctgtggt cttggtgcgg cacaagccag 720

acctacttcg gcagcagacc atctccttgc ctgagctggt ccagcagaat tgtggcccct 780

ggacacgccc tgagctgtcg gactagaaga agatggtcta gcaagcggtg gatcttctcc 840

aaccagctga ccgtgcagat gctgtgcctg ccaatcacac acccattcgc ctctcagcct 900

ctgggacaag agatgcccaa caacacccag atccggctgc tgaagccctg cagagtgaac 960

gccgtggaag aactgcacag caggcggatg tctgtgcgga tcgtgaagaa ctgggtgtcc 1020

1080

aagaagaacg gccggatcag cagcctgaaa aagaaagtga atgcctgcag ctccctgatc 1140

ctgagcttcc agcgcgtgaa aaccggcatg gaaatcctgc tgtggattct gctgaagatg 1200

1260

agctttagcg ccgatttcag cgcccctcag atcattaaga agatgaagaa acggatcatg 1320

gtgcacgagc tgaagaagaa tctgctgctg cctatgtgct ccagcctggc tctgccaaca 1380

ggcctgacat ctctgatcct gcctagcagc gatctggccg tgctgatctc tagctccacc 1440

agccactttc tgatgcggag ccctgtgctg ccaagcagca gactgacttg tgcctctcca 1500

cagctgcccc ggatgtggac ttggtctagc tggctgaaga acgaggggcaa caaccactgg 1560

cctagagtgg aaatgcctac cggctggctg ctcgtcggct acaacaccag aaacacagcc 1620

cagaatcctc ggctgcagca gcacctgaaa cactggcagc agtgcaccag agccagatgg 1680

tgtcaagagc cctgtcagac cctgaccttc ttccatttct tcttcttttt caaaacggtg 1740

tccctgtgtt gccccggctg gtctaaagga caacctcctc cacctggcct gaagccttct 1800

agcctggctt ctcaggtggc cagaaccaca ggcgtgtacc agcacgtgtg gctgatctgt 1860

ttcgtgtttt tcgtgtgtgt ggaaaccggc ttctgtcacg tggcccaagt gggactcgag 1920

agactggtgt ctctgtccgg catcaccgac gtgaaccatt gggcccagcc taagccatct 1980

cctagcaaag gcaccggcat gaacagaccc atcggcctga gagttggcaa aggcgtgtgg 2040

gccagactga tcctggccat gacactgacc ctgaacggca tggccaagaa gtcccagcag 2100

cctctgcagg tcgtgctgag actgggaaac ctgcctccat tcttcagagg cagcgaggtg 2160

ccctttccta gaccttgtcg gaccaagcct gcctgcacaa gcaaggcctg gactcccaga 2220

aaactcgtgg ggagagccgt gcggagaaag ggatctccac tctgggattg ctccaccaca 2280

acaggcacac accacggatg cctgtgggag atcccatctg tgaccctgct ggcccatcct 2340

agaaccgcct ctatcaccct gcctaccaca agcctgaagg gctccagaac cagctgctgc 2400

cctagatctc accccagact gcctaggacc aaatggcccc tgcagtgtct gctgcacagc 2460

ctgggaacaa ccggaacact cgtttgggaa aggggcgtcc tgcagatgag atccctcgaa 2520

gcccattgtc cactgcagac cttccatctg caccactgca ggacccagcc tagctcttgt 2580

cctcctgcct ggattctgta ccaaggacag tggagcggag ccgtcgtggg acttagacct 2640

gtggctcacc atctgcctag acctatccag ggcctgcact gtccacaggc acctcatcaa 2700

cctgctctga gctggggaaa atggcccgtg ggaatgcag gccctgccag atgttgcaga 2760

tggcccagac ggtgccagaa gaaggttcca tcagcctctc catggggcct cgtgctcaaa 2820

aagaaccgga aacagatgct gaaccagaag tgtctggccc cttttagaga gagcaccctg 2880

ggcaatttct ggatgaagct gctccagctg cctgtcgtga tcaacctgaa gagactgcag 2940

aagttcaacc tgcacctgtg ctactaccac agccagagca acagaaacaa gagccgccag 3000

atggaatccc tgggcatgaa gttgcagtcc cagaaatccc agcaccactg tgccctgcgg 3060

aagatcgagt gggccacaaa gttgtctctc ggcggaaaag ccggaccaca ggctcaagag 3120

aaggccacct ctcggattct gaacctgcaa gtgctcaaga agatcctgcg gagctttatg 3180

aagctgtacc actctctggt catgtgcctg cggctgagga ccaagctgga aaaggctctg 3240

agcgccctgt tcatctggcc cggcacagc tacaagcccc tgttctttca cggcgacctg 3300

tgcgagtggg gaggccaggc tcatagacag cggaactggc tgaaaaactg gaagcggttc 3360

aagcttaaaa agaagtacat ccagaagaag aagcagaagt ctaacaagat catctgcaag 3420

ggccaccgca tcaccatcat ctgtatcaag aatatgttcc tgcagcgcaa gggcttcctg 3480

aacctgagcc acgtggaaaa gttctgtaga ggcggcccta gcgccagcga tagatgctgt 3540

tcttgtagcc ccagcggctt cagtgccggc agaggaagat gtcctgttca gggctgtctg 3600

aggccccaca gagttcaact gctgagaaga tggggccctg gctctcctgc tggacagagg 3660

ctgtctaagg gctttcagct gctccgttgg tggggaccag gatctcctgc tcctgagcct 3720

agaaagggcc catttccacc acctgatcct ccatggcctg tgaccgctgt gacagtgatg 3780

gctggatctg tgcctagtgc tcagtctgtg gacgccctgg aatctcctgg acctctggct 3840

3900

ggcatcttca ccgccaagaa aagccccacc agcttttgct gcaatatcct gaacagcctg 3960

ccaagctcta tggaaatcgc cgtgctgcaa gaggacagca aaaagatgac ccagaagggc 4020

caagccttcg gctccctgtg tcagcttgtg tccgctgact tctctgcccc tcaagtgacc 4080

cggctgcgga gcctgaatca tctgctgatc acctacacac cccacatctg cagacacacc 4140

ctgctgaccc ctgacaccag atggatcttt agcaatcagc tgcagacagt ccagatgctc 4200

tgtctgcctc cactgaagca gggcgagaga atcatttggc ccctcggcgg ctttcagatc 4260

cccaacttct tttggagcct cggcatcgga cacactggcg cctggtggca tactggaagg 4320

cggagaagag aatggggcgg cagcaccgtg tctagaagcc ctctgtggat cagctccatg 4380

atctacctga gaggcatggc cttcgccagc gacagcagca tcagaaccac cagagtgtcc 4440

tggtgcagaa gatgcggcgc caaaagcgct gccgccagct ctattgaagc tctgaccgct 4500

ctgtttacct gccggaccgc cagcatcagc gtgaacatca gcaccctgat gtccatcctg 4560

gaagcactta agaagaaacc cagagacatc tgccgcatca gctgccggtg tagacgcgag 4620

tacagagtga ctatgggcta cccctacgat gtccctgact atgcgtccta aaggcgcgcc 4680

tagcggccgc 4690

<210> 1211

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 1C cloning cassette

<400> 1211

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgagcacca gacgggaaat gccttctcag gccacacctt ctccacctgg accttggaga 780

gccgctctgg gcaaagaagt gaagcggagc cagagcaagc tgcggctgag aaacacaagc 840

cccaccgaga tccaattgac catgaacgac agcaagcaca agctggaaag ccccgctctg 900

aaacaggtgt cccctgcctc tccacctaca cagcagcctc agacacccca ggatagcaga 960

caggtgctgg ccgcttcttc tcctggacat cctcttggcc ctggcgactg ccctagaaca 1020

atggctggac cttctgcctc tctgcaccca gcatctggca gagccagcag aaagacctgg 1080

cagagaaggc tgagagcctg tagagctggt ggacctgctg gatggggctag acaggccatg 1140

acactgaagg ccctggtcaa gacctggaca cagttcagaa actaccccag agtcggagcc 1200

1260

gctgaaggcg ctgccggaac aagatgcaga gcttctagag ccccttgcag acagctgcct 1320

gccagctctc ctaccaagtg cgagatctgc gtgaagagaa ggcggcggag aaagcggagc 1380

agccagtcta gatctcccgt gcctaagaaa cgcgagcgga tgatgctgcc tgtgcgggcc 1440

agaacagcta gatctgctct gccacaaggc agagcccctc tggaaggaat gcttctgcct 1500

agagcactgc ccagcctgct gagcagaatg ccagccagaa gagagagccg gttttggctg 1560

gcactgcagg ctctgggact cagacctcct catctgccta gctgtcaccc tctgcctcaa 1620

ctgcttggac ctcctccacc tcatcaggcc cctttcgaca agaaaggctt cgtgatcctg 1680

agcaagggca agaagcgccg gaaagaaatc cggcggatga ccagacggac catgaccacc 1740

accaaaatga tgatgatgcg gcggaccaag cggcggaggc gaaaacgtag aagaatgacc 1800

atgatgaccc gcagaaccct gctgaccaag aaaacctgtc tgacccctag aaccagcgcc 1860

gccacaaaag ctctgcccag acagacaatc agccagcggga ggcctatcca gacacctcct 1920

ggaacaagcc tggccatctg gggcagactg cctctcagac cacagagagg acctcggcct 1980

ctgcatagat ggctgcagca gacaacaggc tgctcccaga gaagaatcgc caagaagcgg 2040

atcaagctga ggcggaacac actggccatc cggtttatct ctgcccctca gcctagcagc 2100

agcaccgtta cagctgctcc tagcggccag tacttcagca gcttcttcta caccagcgac 2160

cggaccaagt gtgaaatctg tgtcaagaac accgccatcc tggctatcac cctgatcacc 2220

cctcaggacc ctcacctgga ccctgtgtgt cacccacata gaaggcctgg ctggaagggc 2280

caccacagag tggtgtttag ccaccatcca agcggctcca ccaagagcca ttggcagcag 2340

agagaaagag gcggaggccg cagagaaaaa aagggctgca gagagatgcc cagcagctgg 2400

tctagctctg gcagaaccgc cgacaaaaag aacgtgcgga gcagaaccag actgaccctg 2460

acaggcacaa gaagcagcgg cacaagagcc tctccaagcg ccgtgtgtct gtgctgttgg 2520

acatgcccct ccagaagctt ccctgccgca tctccatctg gactgagagc acctgttgga 2580

gcccaggctc ctcctgaagt gacactgaga gccagctgtg cctctagcgc ctctaaagcc 2640

tgtgctggac tttggccttg gggagcccaa gtgcggaaca aagtgcggca gaacttcaag 2700

2760

caggtgctgt tcagacggaa tctgacccca agccagctga gaactctggc ccctccttac 2820

atgctgctgc cccagtctct gtctcagagc ctgtccagaa agcagatccc cagccaatca 2880

atgctcgtga ccgccttctt cctgctcgac atcatcctga gaagcctgct gtactcccgg 2940

atgtgcctgg tgcagatccc tacacacacc tgggagcccg actacttcgt gtccctgcct 3000

atgatgttca gctgggccac tcagagcttt ccagtgggag ccgtgttcct gaccagacct 3060

gccattcctt gctgcagctg taccagccac agcaaccctt ggagccagtc cttcaagaag 3120

aagaagcccc tgacattcag ccccagcgtg ccaatgtctc tggcctgtag catcaccgtg 3180

tccttcgtgg gccagatcag cacactggtg ctggacgtgt cctccatctc tcactggcgc 3240

aagcgcaaga gagagaagcg gtcccagaag tcccggcaga accatctgca gctgaagtcc 3300

tgcagacgga agatcagcaa ctggtccaag ctgagatccc cgtggatctt ctaccctcgg 3360

ctgaaaacct tcggcgtgcc actgggctct atcctgtgtc tggcaggctc cctgagcacg 3420

atgatgatca ttagcaccac cagacaagag tgtggcagac gggcctccgt gccttgtaga 3480

cctatgatcg gcagcgctag acccggacct tggcggacat ctgctatgcc atctgccatg 3540

ggagtcgccc tgcctaccag ctgtgaaagc ggaagatctc ctcctgccac cggcggaaga 3600

atgcctggat ctcagtctat catgatgagc tggatgcctc cactggctcc ctgtgccgct 3660

tgtccttgtt ctcctgctcc tacactgtgc ctgctcatc ctgcaagagc ccctacagcc 3720

gtgcctgcct ttacacctct gtctgctcac cctgtgccag tgctgtctgg atgtcacctg 3780

gccgtgcgca cctctatgct gacactgctg cccatgagac accctcacag aagcccacct 3840

acaagctgcc ctccactgct ggaagctttc ccactgccaa gggccagcct gagagctacc 3900

gtgcctagac acagctctgt gctggccact ggactgcatc tgcatcggca gattagcctg 3960

cctctgaggg gcttcctgct gcatcaacct ctgcctggac aacacctgtg gctgtcccac 4020

agactgaggc tgagactgcc aagaacaggc gagaagaacg acctccagct gttcgtgatg 4080

agcgacacca catacaagat ctactggaca gtgatcctgc tgaacccctg cggcaacctg 4140

cacctgaaaa ccacatctct gcggagagcc agacctacct cctgcagcac atgcagaatg 4200

tggtggctga agctggcccc actgcaagtg accacaacac tgaagctgat cctggtcatg 4260

ctggaaatcg tgaccgaaga ggccaagaaa tccatgaaga gatcccggcg gagcaagatc 4320

gaggcctctc ctatcggctc catcatgaag aagaaagaca gcaccatctt catcaaccag 4380

cagctcaagt ggacccctca catcctgaag tctgccagcg tgtccaagaa gagactgccc 4440

aagctgaacg gcatctggga agtgatgccc cggacactgg aaggctgcat cagcgtgaag 4500

gaccaccgga caagccagaa ccacagcaag tgcatcaccg agtgtgtgcg gacagccctg 4560

aaccagagaa aggtggtcaa gaaccccagc cagccagtgc cactgagaaa cgacggcatc 4620

accaacggca acgactccac cagatcctgc tccatcagcc tgaagaaaag cctggaaaag 4680

atgcccctga tgaacgccaa gaaaatggaa gatatgatct gggagagcaa catgggcggc 4740

aaccggtcca tctgcctgac aagactgccc tgcaaagtga ccgagtccag ccaaagacca 4800

ccctcggagca ttctgtccct ggcctctaga gccggacagt ttcccgtttg gcggagaacc 4860

agcagaggca tgtccctgca gtggaagtcc ggcaatgcct gcacacctct gaggtgccct 4920

agcagaagcc ctgccacatt tctgaccgca ctgaacggct ggcacagatc tggacttagg 4980

gctcagcaac ctagcctgcc aggaccttgt gctagcctgc tgtctacact gagccagcct 5040

ccaccacagg ctccaagcca agtttggaca gccgccacac tgagatgtcc cgctgttcct 5100

gcagctgctt gccctcctat cgtgttcttt ctggccccat acttccccac accactgagc 5160

atcagcagac ccacctctcg ggccagacag tcttgttgtc tccccggact gacacaccct 5220

gctcaccagc ctcttggctc tatgggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1212

<211> 5281

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 2C cloning cassette

<400> 1212

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgcggcaga tcaacagacg gaagctgcag agaaagaagc gccggaagaa gagagaggac 780

cactgcggac tgcgggccag aaaccggaac atgaagaaaa ccatgagcca cctgatcgcc 840

catcaggccc tgaagatgtc tggcctgaga ctgtttcacc ccagactgct gacacggaag 900

aagcctggca agaaccctcg gagaaagacc tggaagatgc ggaagaagta cgcccagaag 960

cctagccaga agaaccacct gtacggcaag ggcctgacac accctgtgcc tctgaagaga 1020

accctgcaga acaagcggaa caagaacatc aagcgcgtga accagaagaa gggcaacatc 1080

aagagaaaga gcatcctgct gcacctcatc gtgctgaaca gccccgagag caacctgcag 1140

gctcatcctc aagttccagc actggctcct caggcttggt ggcctgctag aagaggccct 1200

ctgacaagcg ctccatctgc tcagcagagc ctgaccttcc tgcctagcag atttccttgg 1260

ggccctcctc cactgcactg gaactctgcc tacctgggca gaacactgct cgtgacctgc 1320

atcctgtacc acagatggat cgtgaccagc atgtgccaga gccagagatg gctgaccagc 1380

accacctcta gaagcagcgc cctgatgtgg acccagtggt ctagcgtgac aagcctggaa 1440

tggacagccc aagagagggg ctgtagcagc tggctgatga agcagacctg gatgaagtct 1500

tggagcctgc gggaccccag ctacagatcc atcctggaat acgtgtccac cagagtgctg 1560

tggatgccca catctacagt gggagccctg agagccacag ctcctgcaac agctgctcca 1620

caggctcaga cccctcattg gagagccgcc acaacaagca gcatgagcag cagccctaga 1680

gccgagccta caagctctag ctcttgggcc acctgttggc ccagatgttg cagctccagc 1740

tgttactggt ccctgtcctc ttggcctcct gctggtgctg ccgaggccac aaataccagg 1800

accagaagca gagagtccca gcggggcaga atgctgactt ggagaagcag cctgtggctg 1860

cagggcacca gatgttttac aggcgtgcgg acctccagca gacacccttc ttggccttgg 1920

accagatgcc tgagaatgcg gcctcctaga agcaacagcc ggaaaaagct gctcgaggac 1980

atgaagcaaa atggcttcac ccggtacaag aaacggggcg tgatagcctg gcggagagcc 2040

actttcctgc tggccctttg ggagtgcagt ctgccccaag ctagactgtg cctgatcgtg 2100

2160

2220

ggcggctgca ccgcctgtgt tgaaagatgt ccttggaagg gcatctgctg gaagtggcct 2280

gtgacagtgg ccgctctgat cctgattcag cacggcggca tcttcacctt ccggttctgg 2340

gtcgagaagc tgtcctggaa catctgcaag aatctgtccc tgtacctgga caagttcgtg 2400

cagcggctgc tgtacgtgta catcatcgag acaaacgtgt tcgtcctgaa cctgttccac 2460

gtgccagtga atcagatctt catgatggcc ttctacctga agtacaccga gaaggcctgg 2520

gtcatggaac tggaatggct cattcagcag cagtgctacc agttgctgct ggctcatatg 2580

tacggcttca gcacctacgg cacccagaaa ggcgagtgcg tgtgtctgca gtgtctgagc 2640

ggcagctccc tgttcatcct gaagcagcca ctggacctga agatcagcaa gaccttcatg 2700

agcagatccc acctggattt cagcccctat tggatgaaga aagtgaaggc ctctccacag 2760

atcatgcagc ggatgagcgc cgtgaacttc gagacaatca ggcggaagtg gcagatccgg 2820

2880

aagatcacaa tggtgctgac ataccccaac tgcgtggtgc atgtgctgat gttcgtgctg 2940

ttcaaggccg accagagcga gtctagcctg agcagcggaa tctaccctcc tagcacctgt 3000

agcctgcctt gccggggcat tagaatgtgc gccctgtctc accctagctt ctacatcagc 3060

agcaccgtgt gctttctgag caagcactgc atgaccgcct gcaccagaga gaagatcatc 3120

aaccccacca tcagctgccc ctttcagagc cccacaaaga gactgggcca cagagagggc 3180

agcgagagcg ataatctggg cctgctgtac tccttttcctc cttggctgcc catgagatcc 3240

ctgggcgaac cttggagagc tggctttcag tggggagagt ccccttggca agtgatggct 3300

agcctgaccg gcaacagcgt gatcgtgcac aagatcgtgc tggtgtttct gaagtctctg 3360

agcagctaca actgcgagcg gcggatctct ggcatccaga agagagccat cgagttcgcc 3420

gtcctgaagc tgctgaacat ggacctggct agccctcaga ttatgcagag aaccagcgcc 3480

gaccggttcc ggatgaagcg gagaatgcag aacctggata acctgggcgt gccctggcca 3540

ttgcttccag gtgctgctcc tccacataga ctgctgggcc cctctagatg gattctgttt 3600

catgtgcgga acatcgtgta cggcatccaa gtgatgctgt gccggcggag aaacagcaga 3660

aagcagagtc aggtgcagct ggccattcag gtcctgctcg tggacaagca caagtacttt 3720

ctgttcggcc cctatcctat cagcgaggga ctgaccatga ccaccggccg gaacatcaac 3780

agcaactccc tgatgaccaa ctgcctgttc agccccatct tcctgtgcca ccacgccatc 3840

3900

caggtgccaa gtgtggtggc cctgtgcaag ttcctggcca atggcctgtg gttcaacctg 3960

atctttcaga aaaagggcca catgatcttt agccccaagt tcttcctgac ctttagccag 4020

cggaccctgg aatccgccgg actgtttcca tggctgcaca cccagtgccg gaacaaaaag 4080

accaagctgt ggttttccct gatcaacatc caccaccgca agaatcctct gctgcctatg 4140

agaatgagca gaacacctcc tcggagagtg tgcggatctc cacctctgca tctgtgccaa 4200

cctctgagag gcggcaagaa gagaagcaag ttcgccttct ggaagcctcc tagccagggc 4260

atcagctctt gtctgatgac aggctgttgg ggctcctgtg gcagagcttg ttcttgcgga 4320

agaggctgcg gtacacccag aggcgactgg aaaagacacc cacctcgcag agagctgagc 4380

ctggaaccat ggattcctta tctgcaccag caaaagaccc agcggaagcg gatgaagacc 4440

ggcgccattc aagagaaggg cgctatccaa gaaaaaggcg caatgaccaa gcggatctgc 4500

gtggtcaatt tcgaggcctg cagagagatg gaaagcctga gcgcccctga gaagattacc 4560

ctgttcgaag cccacatggc ccggtgcacc tccatcaatg tgctgtgtgt ccgggccagc 4620

ctgattgaga agctgatgaa agaaaagcgc aagatgaagc gcaaccaggt ggcatcccct 4680

caaatcatgc aacggctgtt catcaagggc taccagaaca accacttcac gttccacctc 4740

caaaactttg ccttcttcag ctccttcggc aacaacaagt atatcaacat tctcacccgg 4800

gccttcatcc acacacctag aagccctggc tgttccgtgt acgccaatat gtgctggcgg 4860

aagatgcagt ccctgaatca ccccagcaga ctgaccaact gtcaccccgc ttggctgagc 4920

cccattagtg cttgtcctag gctgctgccc tatccttttc acgtgctggc actggaagtg 4980

acctttccac ctggcacagc catgcctcag atgcaattgc aggcactggc tatgtgggag 5040

aaaggctggg acaagcaaga gaagaaatcc cgcaagctga tgtcctcctg cctggaaaaa 5100

aaggctacct ggatgcggag aacccctggc acaagcctgc agtgttacct gaacacacac 5160

ccctgcacca tgctgctgcc tcagggaatc ccatctctga acgcccagtg gatgttcctg 5220

cggatcggct acccctacga tgtccctgac tatgcgtcct aaaggcgcgc ctagcggccg 5280

c 5281

<210> 1213

<211> 5296

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 3C cloning cassette

<400> 1213

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgcctattc ctccacacct gagacaccct ctgctgctga agaacaagaa gcggagaaag 780

ctgcggccca agagcagaaa gaagggccac accaagaacc ggcggaaggc caagagaaag 840

ccccagatca gaaacagccg cagagccaca cctaccagca agggcagcct gagaagaaag 900

tacctgagag tgggagccgc cgctgctagc tgtcaatctc ctccatctcc tagagagaca 960

acccctagca gcgacgccac cagaagaacc agacctatcg ccgctcagag caagaaaacc 1020

atgacagccg ccggactgac ccctagccag aagaaagatc ctgccggcaa gctgaagaaa 1080

caggtgtccg tgtacagcgg caacctgctg agcttctctc ggagaggcat gaagtctagc 1140

gtgcgcctga agaactgcac caacctgagc gtgcccatga tgctgaccat cctgatctgg 1200

aagcgggtgt tcatcctgct gctgtccgac aagaaatcta tccctcacgt gcccaaggcc 1260

accgccgctc attctgtgac agtgaagctg cagagccggc ggaacagacg gtacagaaag 1320

atctatggcg agctgaagat gtccctgcag gccagcaatg gcatggaagc ctgtggcaca 1380

caccccacac ctagactgtc cgtgtccgtg atcaccatga agctgtggga gaagcacttc 1440

aagggcctga actggaacct ggtcgtgtgg attctgaacc tgaagatgat gctcaagaag 1500

1560

tacaaagagt acttcctgaa gagaatcctc atccgcaagg ccatgacagt gctggccggc 1620

gataagaaag gcctgggcag attcatgaga tgcgtgcaga gcgagacaaa ggccgtcagt 1680

ctgcagctgc ctctgggcag aatggacctg gatggaatgc tgaccgtgat cggccaggga 1740

ctcgtgggca tgacacctag agatctggga acagtggctg gcgccaccgc acagattcag 1800

aggcctattc aggtgcagac aggcggcaga ctgagaggcc ctagaagaaa ggtgctgatg 1860

gtgctgtggc tgcccgccaa tcagagcctg cagaaaagcc aggtgttcca gtgggtgctg 1920

agaaccgagc tgcccctgac ctttagcacc ctgctgtggg acagcagaca cgacctgcct 1980

agaatgctgc ccatcagcag ccctctgcac agaccccaga tgcctttctg gacctgcacc 2040

tttcctgcca ccacatgggg cacatgggag acaccttcta catggggctg gcggaccttt 2100

gccagacagc cttctagacc caccggcaga acccctacca ccagactgat ggctccagtg 2160

ggcagcgtga tgagctgtag cctgctgaca cagccatctc cagccagcgc ctgtacaatg 2220

cctccactgc tgcatcctca gtgggcccct cattctgctg gactttctcc aggcgcttgc 2280

agacctgcct gtacctgcat cagcatgacc acaatcagca gggccacctg gcgcagaaac 2340

ctgcagagaa acctgaaagt gctgcggctg agaatcccca gcctgcggaa gtacagacag 2400

cagaacaccc ggtccaccaa cctcgagaag aacccatctc tgaagaagca gagccagagg 2460

cggggcaagc tgtctctgac cacactgagc ttcgtgctga tcgaggaact gctgaaaaag 2520

agcagccagc tgcggagaag ctggcggaac gtgtccagaa tcagcaacat cgggaagctg 2580

atttggacca agcagcggag cttcctgtct ctgttcccca agctgagagc caccgagcag 2640

atgaccaatg tgggctgtag actggatttt caccccagaa gcagctccgg cgacagagtg 2700

ttcctggtca agatccagag cagcccattc ctggccagat cctggaatac catgcagtgg 2760

aacaacaaga aaatggccat gactgtgatc gctggcgccc accactgtca gtacaagctg 2820

acctacacca aagtgtggaa gatgaattgg aagaaaatga tgatcggcag ccagcacctc 2880

ctgttctccc cactggatag ctctccactc cagctgctgc tgcacccaca cggcaagaga 2940

tgcaacccct gttgtcggct gacatggatg agccacctga atcggaagct gcacctcacc 3000

aggtcctgcc tgatcgacct gaccatgatg atgagccaga aactgtggaa gaatcagcgg 3060

aagcgcatcc ctctgccttc tccaagacag catctgctgc ctcagtatcc tctgcaccct 3120

cctccacctt gtctgctgct tctccaccat agacaccacc tggcattcct cgagatggcc 3180

tgtagctctc agcacaccca cagcatcaag atctggatca gcttcagcca cgagacaatg 3240

agcctgtcca acatcctgat gaagttccgg ctgaccacca ccacaagcag ccccagaaga 3300

agccctgctc tgcgagctag aggcggcaca acaatcggcg aagtgaccag cagcctgttc 3360

tttagccact gcctgtgcgt ggcccctaga tgtcctagat ctgccgtgga catcagcccc 3420

ggacctacat ctccttctct ggcctcttgt gcctctgtgg ccattggaaa gctggcactg 3480

cttggcgcct ctgctccttg tggtgctgct ggcgctagag aggccggaag atttgctgtg 3540

ctgggcacca tggtcatgaa gaacatggaa cacagactga ccatggacag cgccctgagc 3600

3660

gtgtccagcg tgctgaagcc tttcctgttc accgtgccta tcgaggacat gagagagtgc 3720

accctgggac gcaacagaat gaatgtctct tccgtgctga aaccctgtct gatccccgtg 3780

ccaatctgca agaagaaact gctgctcaag ctgctgaggg gcgcctccag ctctaccagc 3840

agagatagca gaagcttttt caccagcgct caccccagca gaccctgcag gatgagactg 3900

taccagatgc tgcagaagca gtgctgctac aacctcgtga tcaagcaccc tctgatcttc 3960

cggatcaacc tgcggagaca gggcaagacc aaagacggcg ttgtgcagag cctgggcttc 4020

ctgaaccagc agatcatcct ccagaaagag ttcttcatct gcctgctgat gttcatcaag 4080

cggtggaagc cagccgccac caagaagggc aagaacagcc cctgctgcca gaaaaaactg 4140

cgggtgcaga accagggcca tctgctgatg attctgctgc acaacagagg cccacctctg 4200

cataccagat tcgagaaaaa gcggcccgag tggctgagca gaaaatccgg cgtgatgaag 4260

tgctgggatc tgtgccctgc cttcagcggc aggattctgc accagccaga aatggccgcc 4320

atgagactga tgcccaagtt cctgaactcc accaacagct ggtggacagt gcacaagaag 4380

agaggcctgt tccaccagat gaagtgtctg accgccgtgg actgtctgca ggccttctgg 4440

gatatgagcg agaagaaacg caagctgcct atgattcgca aaatccagaa gcggcggaga 4500

accgccaacc ggaacatcga gatcacccct cagagaagca aagcccacct gaagaaaacc 4560

tccgccaagg gcgccaagaa aatcaccttc ctgtgcctga gagtcagatg cggaggctgg 4620

ctgtgtggct ggacaccaag aggatggctg aagaagtcca ccaagtccag actgtgcttc 4680

ttctacctgc tgtactacct gtttttcctg accgtgtgcg gcttcacccc tccaccactt 4740

attctgtggc ctcaggcagc ccctggcgag aagctgaagc actgttttaa agcctggggc 4800

gtcgaccctc ctggcagcgt ttggattctg ccctgcttcc tgattcacct gagaaaggtg 4860

gacacccacc acctccaggt ggtcaccaaa acagtgaccg ccacaacagc cgacggccag 4920

aggatgagaa ggctgaagtg caaggcctgc cggctgagat gggctagacc tgaacctgca 4980

gctcaggccc ctcctaagag ccactttcca cctcatccta gcggcccctc cagaaccagc 5040

tctctgcaat tgagaaccta catggaaatg cactacggcc atcaggcccc acaggtggca 5100

atgctgccta aactgctgtg tcggcagatg ctccccactc tgtctaccct gcagcagaga 5160

catcctcagg gcccatgtcc tcctccagtg acacctctga gagtgcagtc tgtgctgctg 5220

ctcggagtgc ctcaaacagc tggctacccc tacgatgtcc ctgactatgc gtcctaaagg 5280

cgcgcctagc ggccgc 5296

<210> 1214

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

728

<223> hINV 4C cloning cassette

<220>

<221> hINV 4C cloning cassette

<222> (1)..(5299)

<400> 1214

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atggacagca agaagatgac ccagaagaac cgcaagaccc ggaaggccgc cagagagaag 780

cacagaaaga ccgacaacct ggacctgcag caccagcgca gacgcaaaaa gaagaaggac 840

aaggtcgaga tccggaatcg gagccctaag gccaatagca ccgagtgtca gggcgagcac 900

agcagagagc agaacctgct gaacctggtg caattgaacc ctcacagccc tcaccaccgg 960

aaggacgagg aagatcacca gaagagacac caccaccaca atcaaaagaa gatgtccgtc 1020

aacgagggca acaaccactg gcctagagtg gaaatgccta ccggctggct gctcgtgggc 1080

tacaacacca gaaacacagc ccagaatcct cggctgcagc agcacctgaa acactggcag 1140

cagtgcacca gagccagatg gtgtcaagag ccctgtcaga ccctgacctt cttccacttc 1200

ttcttctttt tcaaaacggt gtccctgtgc tgccccggct ggtctaaagg acaacctcct 1260

ccacctggcc tgaagccttc tagcctggct tctcaggtgg ccagaaccac aggcgtgtac 1320

cagcacgtgt ggctgatctg cttcgtgttt ttcgtgtgcg tggaaaccgg cttctgtcac 1380

gtggcccaag tgggactcga gagactcgtt agcctgagcg gcatcaccga cgtgaaccat 1440

tgggcccagc ctaagccatc tcctagcaaa ggcaccggca tgaacagacc catcggcctg 1500

agagttggca aaggcgtgtg ggctagactg tggctgaaga actggaagcg gttcaagctc 1560

aaaaagaaat acacaggcat ggaaatcctg ctgtggattc tgctgaagat ggaaattcag 1620

atcttcaaga tttgcctggg cgagatgcag ctgtgcctca agaagaaagt caacgcctgc 1680

agcaacgcca agaaaatgcc cttccagtgc cggtgcagac gcgagtacag agtgaccatg 1740

attcagaaga agaaacagaa gtccccgctg ttcttccacg gcgacctgtg cagaccttct 1800

ccttgcctgt cttggagcag caggatcgtg gctcctggac acgccctgag ctgtcggact 1860

cgtagaagat ggtccagcaa gatggccaag aagtcccagc agcctctgtg tagaggcggc 1920

ccttctgcca gcgatagatg ctgtagctgt agccccagcg gctttagcgc tggaagaggc 1980

agatgtcctg tgcagggatg tctgaggcca cacagagttc agctgctgag aagatggggc 2040

cctggatctc ctgctggcca gagactgtct aagggcttcc aactgctgcg ttggtgggga 2100

ccaggatctc cagctcctga gcctagaaag ggcccatttc caccacctga tcctccatgg 2160

cctgtgaccg ctgtgacagt gatggctggc tctgtgccta gcgctcagtc tgtggatgct 2220

ctggaatccc ctggacctct ggctctcgaa ggccctagca gccctagaaa cctgctctgg 2280

cgggaaatga gcatcttcct gcctggcatc ttcaacctgc acctgtgcta ctaccacagc 2340

cagagcaacc ggaacaagag ccggcagatg gaatccctgg gcatgaagct gcagatcaca 2400

cacccattcg ccagccagcc actgggccaa gagatgccca acaacaccca gatcagactg 2460

ctgaagcccg gccaggcctt tggcagcttt tccgccgatt tttcagcccc tcagcaggcc 2520

cctctgagac tgtggtcttg gtgcggcaca agccagacct acttcggctc tatcgaagcc 2580

ctgacagccc tgttcacctg tagaaccgcc agcatcagcg tgaacatcag caccctgatg 2640

agcatcctgg aagccatcct ggccatgaca ctgaccctga atggcagccc tctgtgggac 2700

tgcagcacaa caacaggcac acaccacggc tgcctgtggg agatcccttc tgttaccctg 2760

ctggctaccg ccaaaaagag ccctaccagc ttctgctgtc agcggcctcc tctgcagcca 2820

gtggaagaga agaagaacgg ccggatcagc agcgtgccac cttctccacc tcttgctctg 2880

ggccctagaa tgcagctctg cacccagctg gcccggttct tccctattac acctcctgtg 2940

tggcacatcc tgggacctca gagacacacc cctctgctga cccctgacac cagatggatc 3000

ttctccaacc agctgcagac cgtgcagatg ctgtgtctgc ctccactgaa gcagggcgag 3060

agaatcatct ggcctctcgg cggctttcag atccccaact tcttttggag cctcggcatc 3120

ggacacactg gcgcctggtg gcatactgga aggcggagaa gagaatgggg cggcagcacc 3180

gtgtctagaa gcccactgtg gatcagctcc atgatctacc tgagaggcat ggccttcgcc 3240

tccgacagca gcatcagaac caccagagtg tcctggtgtc ggagatgtgg cgccaaaagc 3300

gccgcagcca gcagcgacaa gaagaagaaa agcaccgaaa agaccaagaa caagatcatc3360

tgcaagggcc acagaatcac ccaggtggtg ctgagactgg gcaacctgcc tccattcttc 3420

agaggcagcg aggtgccctt tcctagacct tgccggacca agtgccagaa gaaggttcca 3480

agcgcctctc cttggggact gcacccaaga acagcctcca tcacactgcc taccaccagc 3540

3600

tggcctctgc agtgcctgct gcacagcctg ggaacaactg gaacactcgt gtgggaaagg 3660

ggcgtgctgc agatgagaag tctggaagct cactgccctc tgcagacatt ccacctccac 3720

3780

agcggagccg tcgtgggact tagacctgtg gctcaccatc tgccaagacc tatccagggg 3840

ctgcactgtc ctcaggctcc tcatcaacca gctctgtcct ggggaaagtg gcccgtggga 3900

atgacaggac ccgccagatg ttgccggtgg cctagaagaa tgtgtagctc tctggccctg 3960

ccaaccggcc tgacatctct gatcctgcct agcagtgatc tggccgtgct gatctctagc 4020

tccaccagcc actttctgat gcggagccca gtgctgccaa gcagcagact gacatgtgcc 4080

tctccacagc tgcctcggat gtggacatgg tctagctggc tgaaagagtg gggaggacag 4140

gcccacagac agcggaactg tagagtgaac gccgttgagg aactgcacag caggcggatg 4200

4260

ctgtctactt ggagcgccct ggattccctg atcctgtcct tccagagagt gaaaaccagc 4320

cggatactga acctccaggt gctgaagaag atcctgcgga gctttatgaa gctgtaccac 4380

tctctcgtga tgtgcctgcg gctgcggacc aagctggaaa aagctctgag cgctctgttt 4440

atctggcccc agcacagcta caaaggccag gcattcggaa gcctgtgcca gctggtgtct 4500

gccgatttca gcgcccctca agtgaccaga ctgcggagcc tgaaccatct gctgattcgg 4560

tggatcttta gcaatcagct gacagtccaa atgctctgcc tgccgatcat caagaaaatg 4620

aagaaacgga tcatggtgca cgtcttgaag aagaatcgga agcagattat ctgtatcaag 4680

aatatgttcc tgcagcggaa gggattcctg aacctgagcc acgtggaaaa gttcagagag 4740

agcaccctgg gcaatttctg gatgaagctc ctgcagctgc caccagcttg cacctctaag 4800

gcctggacac ccagaaagct tgtggggaga gccgtgcgga gaaagggcgt cgtgatcaac 4860

ctgaagagac tgcagaaatt ccgggctagc gtgtccttct ggactctgct gcctacaaca 4920

ggcgtgcgga caagaggctc tccactgaga ctgactctgt ggcctccaag aagccagaaa 4980

agccagcacc actgcgccct gagaaagatc gagtgggcca caaagctgtc cctcggcggc 5040

aaagcaggac cacaggctca agagaaggcc aacatcctga acagcctgcc ttcctctatg 5100

gaaatcgccg tgctgcaaga aatgctgaac cagaagtgtc tggccccttt cgagcttaag 5160

aagaacctcc tgctgccaac ctacacaccc cacatctgtc ggcacaccct taaaaagaag 5220

cccagagaca tctgcagaat cagcggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1215

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 1D cloning cassette

<400> 1215

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atggccgaca agaaaaacgt gcggagcgct agttctcctg gacatcctct tggccccaga 780

agaatgacca gacggaccat gaccaccacc aaaatgatga tgatgcggcg gaccaagcgg 840

cggagaagaa aaagacgccg gatgaccatg atgaccagaa gaaccctgct gaccaagaaa 900

acctgtctga cccctagaac cagcgccgcc acaaaagctc tgcccagaca gacaatcagc 960

cagcgggaggc ctatccagac acctcctgga acaagcctgg ccatctgggg cagaagaagc 1020

aagatcgagg cctctcctat cggcagcatc acccctctga gcatcagcag acctaccagc 1080

agagccagac agagctgttg ctgcctgga ctgacacacc ctgctcatca gcctctgggc 1140

tctatggttg gagctggaac aggcggagga cctgcttgtg tgcctgtggc cgaagcttct 1200

acaggcgctt ggacagctga aggcgctgcc ggcacaagat gtagagccag cagagcccct 1260

tgcagacagc tgcctgcttc tagccctacc aagtgcgaga tctgcgtgaa gagaagaagg 1320

cggcgcaagc ggagcagcca gtctagatct cccgtgccta agaaacgcga gcggatgatg 1380

ctccctgtgc gggccagaac agctagatct gctctgccac aaggcagggc tcctctggaa 1440

ggaatgcttc tgcctagagc actgcccagc ctgctgagca gaatgcctgc cagaagagag 1500

agcagattct ggctggccct gcaggccctt ggactcagac ctcctcatct gcctagctgt 1560

caccctctgc ctcaactgct tggacctcct ccacctcatc aggcccctgg cgagaaaaac 1620

gacctgcagc tgttcgtgat gagcgacacc acctacaaga tctactggac cgtgatcctg 1680

ctgaacccct gcggcaacct gcacctgaaa accaccagcc tgaacgccaa gaaaatggaa 1740

gatatgatct gggagagcaa ccagcagctg aagtggaccc ctcacatcct gaagtccgcc 1800

tctatcgcca agaagcggat caagctgcgg cggaacagca ccagacggga aatgccttct 1860

caggccacac cttctccacc tggaccttgg agagccgctc tgggcaaaga agtgaagcgg 1920

agccagagca agctgagact gagaaacaca agccccttcg acaaaaaggg ctttgtgatc 1980

ctgagcaaca tgggcggcaa ccggtctatc tgcctgacca tcgtgttctt tctggcccct 2040

tacttcccca tgcctagcag ctggtctagc agcggcagaa ccagactgcc tctgaggcca 2100

cagagaggcc ctagacctct gcatagatgg ctgcagcaga ccaccggctg ctctcagaga 2160

agaggcgact gcccaagaac catggccgga ccatctgctt ctctgcaccc tgcatctggc 2220

agagcctcca gaaagacctg gcagagaagg ctgagagcct gtagagcagg cggacctgct 2280

ggatgggcta gacaggccag aaaacgcaag agagagaagc ggtcccagaa gtccagacag 2340

aaccatctgc agctgaaaag ctgcagacgg aagatcagca actggtctaa gctgcggagc 2400

ccctggatct tcgaggccaa aaagagcatg aagagatccc agcggcctcc tcggagcatc 2460

ctgagcctgt tcaagaagaa gaagcccctg acattcagcc ccagcgtgtc caagaagagg 2520

ctgcctaagc tgaagggcaa gaaacgccgg aaagaaatcc agcggaaggt ggtcaagaac 2580

cccagccagc ctgtgccact gagaaatgac ggcatcacca acggcaacga cagcacccgg 2640

tcttgcagca tcagcctgaa gaaaagcctg gaaaagatgc ccctgatgcg gagcctgctg 2700

tactccagaa tgtgcctggt gcagatcccc acacacacct gggagcccga ctactttgtg 2760

2820

accagcctg ccattccttg ctgtagctgt accagccact ccaatccttg gagccagtcc 2880

acactggcca tccggtttat ctctgcccct cagccaagca gcagcaccgt tacagctgct 2940

cctagcggcc agtattttgc cctgcggagt atgcccaaga gccggaacca ggtgctgttt 3000

cggagaaatc tgaccccaag ccagctgcgg acactggccc ctccatatat gctgctgcct 3060

cagtctctgt cccagagcct gtctcggaag cagatcccat ctcagtccat gctcgtgcgg 3120

accaggctga cactgacagg cactagaagc tccggcacca gagcctctcc atctgccgtg 3180

tgtctgtgct gctggacatg ccccagcaga agctttcctg ccgcctctcc aagcggactg 3240

agagcacctg ttggagcaca ggctcctcca gaagtgacac tgagagccag ctgtgccagc 3300

tccgccagca aagcttgtgc aggactttgg ccttggggag cccaagtgcg gatgaagaag 3360

aaggacagca ccatcttcat ctaccccagg ctgaaaacct tcggcgtgcc actgggcagc 3420

3480

tgcggcagac gggccagcgt gccctgtaga cctatgatcg gaagcgccag acctggacca 3540

tggcggacaa gtgctatgcc ttctgccatg ggagtcgccc tgcctaccag ctgtgaaagc 3600

ggaagatctc ctcctgccac cggcggaaga atgccaggat ctcagtctat catgatgagc 3660

tggatgcctc cactggctcc ctgcgctgct tgtccttgtt ctcctgctcc tacactgtgc 3720

cctgctcacc ctgctagagc accaacagcc gtgcctgcct ttacacccct gtctgctcat 3780

cctgtgccag tgctgtctgg atgtcacctg gctgtgcgga cctccatgct taccttgctg 3840

ccaatgatga ccctgaaggc cctggtcaag acatggaccc agttcagaaa ctaccccaga 3900

3960

ccaagagcca gcctgagagc taccgtgcct agacactcta gcgtgctggc cacaggactg 4020

catctgcata ggcagattag cctgcctctg cggggcttcc tgctgcatca accactgcca 4080

ggacaacacc tgtggctgtc ccacagactg agactgcggc tgcctagaac aaccgagatc 4140

cagctgacca tgaacgactc caagcacaag ctggaaagcc ccgctctgaa acaggtgtcc 4200

ccagctagtc ctcctacaca gcagcctcag acaccccagg attctagaca ggtgctggcc 4260

gtgcctatga gcctggcctg tagcatcacc gtgtccttcg tgggccagat ctctaccctg 4320

gtgctggacg tgtcctccat cagccactgg aacggcatct gggaagtgat gcccagaaca 4380

ctggaaggct gcatctccgt gaaggaccac cggacatccc agaaccacag caagtgcatc 4440

accgagtgtg tgcggacagc cctgaacacc aagtgtgaaa tttgtgtgaa gaacaccgcc 4500

atcctggcta tcaccctgat caccccacag gaccctcacc tggaccctgt gtgtcatccc 4560

catagaaggc ccggctggaa gggacaccac agagtggtgt ttagccacca tccttccggc 4620

agcaccaagt ctcactggca gcagagagaa agaggcggag gccgcagaga aaagaagggc 4680

tgtagagagg caagcagagc cggccagttt cctgtgtggc ggagaacatc tagaggcatg 4740

tccctgcagt ggaagtccgg caatgcctgc acacctctga gatgtcccag cagatctcca 4800

gccaccttcc tgactgccct gaacggctgg catagatctg gactgagggc ccagaccgcc 4860

ttctttctgc tggacatcat cctgcggaga gcccggccta caagctgcag cacatgtaga 4920

atgtggtggc ggctgccctg caaagtgacc gaaagctccc tggccaagaa aaccctgacc 4980

aatcagccta gcttgcccgg accttgtgct agcctgctgt ctacactgag ccagccacca 5040

cctcaggctc ctagccaagt ttggacagcc gctacactgc ggtgtcctgc tgtgcctgct 5100

gctgcatgtc cacctctgaa gctggctcca ctgcaagtga ccacaacact gaagctgatc 5160

ctggtcatgc tggaaatcgt gaccgagaac aaagttcggc agaacttcaa gcggaactcc 5220

agcttcttct acaccagcga cagaggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1216

<211> 5281

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 2D cloning cassette

<400> 1216

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctagggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgcggaaga tgcagagcct gaatcacccc agcagactga ccaacaatcc tcggagaaag 780

acctggaaga tgcggaagaa gtacgcccag aagcctagcc agaagaacca cctgtaccgg 840

cagatcaacc ggcgcaagct gcagagaaag aagtccagaa gccacctgga cttcagcccc 900

tactggatga agaaagtgaa gcgggccaga aaccggaaca tgaagaaaac catgagccac 960

ctgatcgccc atcaggccct gaagatgtct ggcctgagac tgtttcaccc cagactgctg 1020

accagaaaga agcccggcaa gcagaaaaag ggccacatga tcttcagccg gcggaagtgg 1080

cagatccggc acgtgctgaa gaatctgatc cccagcctga acgcccagtg gatgttcctg 1140

agaatcgcta gcccacagat catgcagaga accagcgccg accggttccg gatgaagcgg 1200

agaatgcaga acctggacaa tctgggcgtg ccctggcctc tgttgcctgg attgctgctg 1260

gcccatatgt acggcttcag cacctacggc acccagaaag gcgagtgcgt gtgtctgcag 1320

tgtctgagcg gcagcagcct gttcatcctg aagcagcccc tggacctgaa gatctccaag 1380

accttcatgg ccagccctca gattatgcag cggatgagcg ccgtgaactt cgagacaatc 1440

ctgcaggccc atcctcaggt gccagctctt gctcctcaag cttggtggcc cgctagaaga 1500

ggccctctga catctgctcc aagcgcacag cagtccctga cacggacact ggaaagcgcc 1560

ggactgtttc cttggctgca cacccagtgc cgggccttca ttcacacacc tagaagccct 1620

ggctgcagcg tgtacgccaa catgtgctgg cggtacaaga aacggggagt gatcgcctgg 1680

cggatctacc ctcctagcac atgcagcctg ccttgcagag gcctgatcct gattcagcac 1740

ggcggcatct tcaccttccg gttctgggtc gagaagctga gctggaacat ctgcaagaac 1800

ctgagcctgt acctggataa gttcgtgcag cggctgctgt acgtgtacat catcgagaca 1860

aacgtgttcg tgctgaacct gttccacgtg ccagtgaacc agatcttcat gatggccttc 1920

tacctgaagt acaccgagaa ggcctgggtc atggaactgg aatggctgat ccagcagcag 1980

tgctaccagc ggaagagaat gaagaccggc gccatccaag agaagggcgc tattcaagag 2040

aaaggggcca tgaccaagcg gatctgcgtg gtcaatttcg aggcctgcag agagatggaa 2100

agcctgagcg cccctgagaa gatcaccctg tttgaagccc acatggcccg gtgcaccagc 2160

atcaatgtgc tgtgtgtcag ggccagcctg atcgagaaac tgatgaaaga aaagcgcaag 2220

atgaagcgca accaggtggc atccctcaa atcatgcaac ggctgttcat caagggctac 2280

cagaacaacc acttcacgtt ccacctccaa aacttcgcct tcttcagctc cttcggcaac 2340

aacaagtata tcaacatcct gacctttctg cccagcagat tcccttgggg acctcctcca 2400

ctgggagccc ttagagctac agctcctgct acagctgctc cccaggcaca gacacctcat 2460

tggagagccg ccaccacaag cagcatgagc agctctccaa gagccgagcc taccagctct 2520

agctcttggg ccacttgctg gcctagatgc tgcagctcca gctgctattg gagcctgtct 2580

agctggcctc ctgctggcgc tgccgaagcc acaaatacca gaaccagaag ccgcgagagc 2640

cagcggggaa gaatgctgac ttggagaagc tccctgtggc tgcagggcac cagatgtttt 2700

acaggcgtgc ggaccagctc caggtgcagc ctgatggaac agatccctca cctgaagtct 2760

aacagccgga agaaactgct cgaggacatg aagcagaacg gcttcaccag acggcggaac 2820

agcagaaagc agagccaagt gcagctggcc atccaggtgc tgctggtgga caagcacaag 2880

tactttctgt tcggcagagc cacatttctg ctggctctgt gggagtgtag cctgcctcag 2940

gctagactgt gcctgatcgt gtctagaacc ctgctgctcg tgcagtctag acacccaagc 3000

3060

ctggggagaa cactgctcgt gacctgcatc ctgtaccaca gatggatcgt gaccagcatg 3120

tgccagagcc agagatggct gacctccacc acctctagaa gctctgccct gatgtggacc 3180

tggtggtgta caagaggtgg cggcaagcgg aagaaaaaga tgtgcaaggt gtccggcggc 3240

tgcaccgcct gtgttgaaag atgtccttgg aagggcatct gttggaagtg gcctgtgaca 3300

gtggccgcca tgagcagaac acctccaaga cgcgtgtgcg gccacagaga gggcagcgag 3360

tctgataatc tgggcctgct gtactccttt ccacctgtgc tgttcaaggc cgaccagagc 3420

gaaagctctc tgagcagcgg caacagcgtg atcgtgcaca agatcgtgct ggtgttcctg 3480

aagtccctga gcagctacaa ctgcgagaga agaatcagcg gcatccagaa gagagccatc 3540

gagttcgccg tcctgaagct gctgaacatg gacctcggca agggactgac acaccccgtg 3600

3660

aagggcaaca tcaagaggaa gtccatcctg ctgcatctga ttgtgctgaa cagccccgag 3720

agcaaccggg aaaagatcat caaccccacc atcagctgcc cctttcagag ccccacaaag 3780

agactgggaa gccctcctct gcacctgtgt cagcctctga ggccctatcc tatcagcgag 3840

ggcctgacca tgaccaccgg cagaaacatc aacagcaact ccctgatgac caactgcctg 3900

ttcagcccca tcttcctgtg ccaccacgcc atcatgcacc ggaagatgac aagccctcac 3960

ttcagactgt tctccagcaa gatcccacat ccacaggtcc caagtgtggt ggccctgtgc 4020

aagtttacac acccctgcac catgctgctg ccccaaggcg ctttttggaa gcctcctagc 4080

cagggcatct ccagctgtct gatgacaggc tgttggcagt ggtccagcgt gacaagcctg 4140

gaatggacag cccaagagag aggctgttcc tcctggctga tgaagcaaac atggatgaag 4200

tcttggtccc tgcgggaccc cagctacaga tccatcctgg aatacgtgtc cacacggggtg 4260

ctgtggatgc ccacctctac cgtgcctaag ttctttctga ccttcagcca gatccggatg 4320

tgcgccctgt ctcaccctag cttctacatc agctccaccg tgtgctttct gagcaagcac 4380

tgcatgaccg cctgcacacc tggaaccagc ctgcagtgtt acctgaacct ggccaacggc 4440

ctgtggttca acctgatctt ccggaacaaa aagaccaagc tgtggttctc cctgattaac 4500

atccaccacc gcaagaaccc actgctgccc atgcggctgg aaaaaaaggc cacttggatg 4560

cggcggaccg agctgtctct ggaaccttgg attccttacc tgcatcagca gaaaacccag 4620

tggctcccca tgagaagcct gggcgagcct tggcgagctg gatttcagtg gggagagtct 4680

ccctggcaag tgatggcctc tctgacaggc agacgcaaga aaagagagga tcactgcgga 4740

ctgcaagaga aaaaaagccg gaagctgatg tccagctgcg ccgctcctcc acatagactg 4800

ctgggaccta gcagatgggg aagctgtggg agagcctgtt cttgtggcag aggctgtgga 4860

acccctagag gcgactggaa gagacaccca cctcggcgga ttctgtttca cgtgcggaac 4920

atcgtgtacg gaatccaagt gatgctgtgc tgtcaccccg cctggctgtc tccaatcagc 4980

gcttgtcctc gcctgctgcc ttatccattc cacgttctgg ccctggaagt gacattccct 5040

ccaggcactg ccatgcctca gatgcaattg caggcactgg caatgtggga gaaaggctgg 5100

gacaagcctc tggaaaacac acagatcccc aggacaagcc cactgtggaa gatcacaatg 5160

gtgctgacat accccaactg cgtggtgcac gtcctgatgt tcggcggcaa gaagcggagc 5220

aagttcggct acccctacga tgtccctgac tatgcgtcct aaaggcgcgc ctagcggccg 5280

c 5281

<210> 1217

<211> 5296

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 3D cloning cassette

<400> 1217

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgcagaaca ccagaagcac caacctggaa aagaacccca gcctgaagaa gcagagccag 780

cggagaggaa agctgagcag cagaaagaag ggccacacca agaaccggcg gaaggccaag 840

agaaagcccc agatcagcca gaagaaggac cctgccggca agctgaagaa acacctgaac 900

cggaagctgc acctgaccag atcctgcctg atcgacctga ccatgatgat gagccagaag 960

ctgtggaaga accagcgcaa gagaatccct ctgccttctc cacggcagca tctgctgcct 1020

cagtatccac tgcatcctcc acctccatgc ctgctgctgc tccaccacag acaccatctg 1080

gcctttctcg agatggcctg cagcagccag cacacccaca gcatcaagat ctggatcagc 1140

ttcagccacg aggacctgcc tagaatgctg cccatcagca gccctctgca cagaccccag 1200

atgcctttct ggacctgcac ctttcctgcc accacatggg gcacatggga gacaccttct 1260

acatggggct ggcggacatt cggccctgtg tactacgtgt gcaaccggat gaacgtgtcc 1320

agcgtgctga agcctttcct gttcaccgtg cctatcgagg acatgagaga gtgcaccctg 1380

ggcagaaaca gaatgaatgt gtcctctgtg ctgaaaccct gtctgatccc cgtgccaatc 1440

ctgaccacac tgagcttcgt gctgatcgag gaaagccggc ggaacagacg gtacagaaag 1500

atctacgggg agctgaagat gtccctgcag gccctgcctc tgacctttag cacactgctg 1560

tgggacagca gacacaccac caccacaagc agccccagaa gatcccctgc tctgagagct 1620

agaggcggca ccacaatcgg cgaagtgaca agcagaggcg ccagcagctc taccagcaga 1680

gacagcagaa gctttttcac cagcgctcac cccagcagac cctgcagaat gagactgtac 1740

cagagcaacg gcatggaagc ctgcggcaca caccctacac ctagactgtc tagaggccct 1800

ccactgcaca ccagaaccat gagcctgagc aacatcctga tgaagttccg gctgatgctg 1860

cagaagcagt gctgctacaa cctcgtgatc aagcaccctc tgatcttccg gatcaacctg 1920

cggagacagg gcaagaccaa agacggcgtt gtgcagagcc tgggcttcct gaaccagcag 1980

atcatcctgc agaaagagtt cttcatctgc ctgctgatgt tcatcaagcg gtggaagcca 2040

gccgccacaa agacacctcc accactgatt ctgtggcctc aggccgctcc tgtgcacaag 2100

aagagaggcc tgttcagcct gttcttctcc cactgcctgt gccaccagat gaagtgcctg 2160

accgccgtgg attgtctgca ggccttctgg gacatgatga ttcgcaagat ccagaagcgg 2220

cggagaaccg ccaaccggct gaaaaagaaa tacctggtgc agtgtaccaa cctgagcgtg 2280

cccatgatgc tgaccatcct gatctggaag cgggtgttca tcctcctgct gagcgacaag 2340

aagtgtctga gagtcagatg cggcggatgg ctgtgtggct ggacacctag aggcaagaac 2400

2460

atcaccatga agctctggga gaagcacttc aagggcctga actggaacct ggtcgtgtgg 2520

attctgaacc tgaagatgat gtggctcaag aagtccacca agagcagaca gagcagccca 2580

ttcctggctc ggagctggaa taccatgcaa tggaacaaca agaaaatggc catgaccgtg 2640

atcgctggcg cccaccactg tcagtacaag ctgacctaca ccaaagtgtg gaagatgaat 2700

tggaagaaaa tgatgatcgg ctcccagcac ctcctgttta gcccactgga ttcaagccca 2760

ctgcagttgc tgctgcaccc tcacggcaag agatgcaacc cttgctgcag actgacctgg 2820

atgagcagag agtgggaaaa gcggaagcag atcctcggcg gaggcggcaa gtacaaagag 2880

tacttcctga agcggattct catccggaag gctatgacag tgctggccgg cgataagaaa 2940

ggcctgggaa gattcatgag atgcgtgcag agcgagacaa aggccgtgtc tctgcagctg 3000

cctctcggcc gaagaaagtc cggcgtgatg aagtgttggg atctgtgccc cagactggac 3060

tttcacccca gatctagctc tggcggcgag aagctgaagc actgtttcaa agcctggggc 3120

ctgtgcttct tctacctgct gtacttcgag aagaaacggc ccgagtggct gagcgttgac 3180

ccctcctggat ctgtctggat tctgccctgc atggacctgg acggaatgct gacagtgatc 3240

ggccagggac tcgtgggcat gacccctaga gatctgggaa cagtggctgg cgccaccgca 3300

3360

aaggtggaca cccaccatct gcaggtcgtg accaagaccg tgacagccac aacagctgac 3420

ggccagagga tgcggagact gaagtgcaag gcctgtcggc tgagatgggc cagacctgaa 3480

cctgctgcac aggctcctcc aaagagccac tttccacctc atcctagcgg cccctccaga 3540

acctctagcc tgcagctcag acccattcct ccacacctga gacacccact gctgctgaag 3600

3660

atgatcctgc tgcataacgc cagacagccc agcaggccta cgggaagaac ccctaccaca 3720

agactgatgg cccctgtggg cagcgtgatg tcttgtagcc tgctgaccca gccatctcct 3780

gccagcgcat gtacaatgcc accactcctg catccacagt gggccccaca tagtgctgga 3840

ctgtctccag gcgcttgcag acctgcctgt acctgcatca gcatgaccac catctccaga 3900

gccacatggc ggctgagaat cccaagcctg aggaagtacc ggcagaacat cgagatcacc 3960

cctcagcggt ctaaggccca ggtttccgtg tacagcggca atctgctgag cttctccaga 4020

cggggcatga agagtagcgt gcggctgaag aacagcatcc ctcacgtgcc aaaagccacc 4080

gccgctcact ctgtgacagt gaagctgcag tacctgtttt tcctgaccgt gtgcggcttc 4140

gaccgggtgt tcctggttaa gatctgcaag aagaagctgc tcctcaagct gctgagaggc 4200

cccagacgga aagtgctgat ggtgctttgg ctgcccgcca accagagtct gcagaaaagc 4260

caggtgttcc agtgggtgct gcggaccgaa acctacatgg aaatgcacta cggccatcag 4320

gcccctcagg tggccatgct gcctaaactg ctgtgtagac agatgctccc cactctgtcc 4380

acactccagc agaggcatcc tcagggacct tgtcctcctc ctgtgacacc tctgagagtg 4440

cagtctgtgc tgctcctggg agtgcctcag acagccgctt ttagcggccg gattctgcac 4500

cagccagaaa tggccgccat gagactgatg cccaagttcc tgaactccac caacagctgg 4560

tggacagtgg cccctagatg tcctagaagc gccgtggaca ttagccccgg acctacatct 4620

cctagcctgg cctcttgtgc ctctgtggcc attggaaaac tggcactgct gggcgcatct 4680

gccccttgtg gcgcagctgg tgctagagag gctggaagat ttgccgtgct gggcaccatg 4740

gttatgaagg gtgctgccgc agcctcctgt cagtctcctc catctccaag agagacaacc 4800

cctagcagcg acgccaccag acggacaaga cctattgccg ctcagagcaa aaagaccatg 4860

actgccgccg gactgacccc tcggagaaac ctgcagagaa atctgaaggt cctgggcgcc 4920

aagaagatca ccttcctgag aaacagcaga cgcgccacac ctacctctaa gggcagcctg 4980

agaagaaagt acctgagagt gaacatccgc aagctcatct ggaccaagca gcggagcttc 5040

ctgtctctgt tccccaagct gagagccacc gagcagatga ccaatgtggg atgcctgctc 5100

aagaaaagca gccaattgcg gcggagctgg cgcaatgtgt cccggatcag caacatggaa 5160

cacaggctga ccatggacag cgccctgagc ctgacaaaga gagagggccc tgagacaagc 5220

gagaagaaga ggaagttgcc tggctacccc tacgatgtcc ctgactatgc gtcctaaagg 5280

cgcgcctagc ggccgc 5296

<210> 1218

<211> 5299

<212> DNA

<213> Artificial sequence

<220>

<223> hINV 4D cloning cassette

<400> 1218

gaattcggat cccgcgactt cgccgccatg catcgacggc gctcacggag ttgccgggaa 60

gaccaaaagc ctgtgatgga cgatcagcgg gaccttattt ctaacaatga acaacttccg 120

atgcttggta ggcggcctgg agcaccagag tccaagtgtt ctaggggcgc cctgtacact 180

ggtttctcca tcctcgtgac actgttgctg gcgggacagg caacaactgc gtatttcttg 240

tatcagcagc aaggaagact ggataagctt acagttacat cccagaatct ccaacttgaa 300

aacctcagaa tgaagcttcc gaagccccca aagcccgtct ccaaaatgag gatggcgaca 360

ccgctcctta tgcaagccct tcccatgggg gctctgcccc aagggccaat gcagaacgct 420

acaaagtatg gaaatatgac agaggatcat gtcatgcatt tgcttcaaaa cgctgatccc 480

cttaaagttt accctcctct gaaggggagc tttccagaaa atcttcgaca tctcaagaac 540

acgatggaaa cgattgactg gaaggtgttt gagagctgga tgcaccattg gctcctgttc 600

gagatgtccc ggcattctct ggagcaaaag ccgacggacg cacctcccaa ggaaagcctg 660

gaattggagg acccgagtag tgggctgggc gtcacaaagc aagacctggg acccgtgccg 720

atgaacagaa agaccagaaa ggccgccaga gagaagcacc gcaagaccga cgacaagaag 780

aagaagtcca ccgaaaagac caagaacgcc aagaagatgc ccttccagca gcggcctcct 840

ctgcagcctg ttaacctgga tctgcagcac cagcgcagac gcaaaaagaa gaaagacaag 900

gtcgaaatcc ggaatcggag ccctaaggcc aatagcaccg agtgtcaggg cgagcacagc 960

agagagcaga acctgctgaa tctggtgcag ctgaaccctc acagccctca ccacagaaag 1020

gacgaagagg accaccagaa gcggcaccac caccacaatc aaaagaaaat gagcgtgcgg 1080

gctagcgtgt ccttctggac acttttgcca acaaccggcg tgcggacaag aggctctcca 1140

ctgagactga cactgtggcc tccacggggtt ccaccttctc cacctcttgc tctgggcccc 1200

agaatgcagc tgtgtaccca gctcgcccgg ttcttcccta tcacacctcc tgtgtggcac 1260

atcctgggac ctcagagaca cacacctcag gctcctctga ggctgtggtc ttggtgcggc 1320

acaagccaga cctacttcgg cagcagacca tctccttgcc tgagctggtc cagcagaatt 1380

gtggcccctg gacacgccct gagctgtcgg actagaagaa gatggtctag caagcggtgg 1440

atcttctcca accagctgac cgtgcagatg ctgtgcctgc caatcacaca cccattcgcc 1500

tctcagcctc tgggacaaga gatgcccaac aacacccaga tccggctgct gaagccctgc 1560

agagtgaacg ccgtggaaga actgcacagc aggcggatgt ctgtgcggat cgtgaagaac 1620

tgggtgtcct gctgttacgt gcggctgtct gctgtgggcc tgtctacttg gagtgccctg 1680

gacgaggaaa agaagaacgg ccggatcagc agcctgaaaa agaaagtgaa tgcctgcagc 1740

tccctgatcc tgagcttcca gcgcgtgaaa accggcatgg aaatcctgct gtggattctg 1800

ctgaagatgg aaattcagat cttcaagatt tgcctgggcg agatgcagct ctgcggccag 1860

gcctttggaa gctttagcgc cgatttcagc gcccctcaga tcattaagaa gatgaagaaa 1920

cggatcatgg tgcacgagct gaagaagaat ctgctgctgc ctatgtgctc cagcctggct 1980

ctgccaacag gcctgacatc tctgatcctg cctagcagcg atctggccgt gctgatctct 2040

agctccacca gccactttct gatgcggagc cctgtgctgc caagcagcag actgacttgt 2100

gcctctccac agctgccccg gatgtggact tggtctagct ggctgaagaa cgagggcaac 2160

aaccactggc ctagagtgga aatgcctacc ggctggctgc tcgtcggcta caacaccaga 2220

aacacagccc agaatcctcg gctgcagcag cacctgaaac actggcagca gtgcaccaga 2280

gccagatggt gtcaagagcc ctgtcagacc ctgaccttct tccatttctt cttctttttc 2340

aaaacggtgt ccctgtgttg ccccggctgg tctaaaggac aacctcctcc acctggcctg 2400

aagccttcta gcctggcttc tcaggtggcc agaaccacag gcgtgtacca gcacgtgtgg 2460

ctgatctgtt tcgtgttttt cgtgtgtgtg gaaaccggct tctgtcacgt ggcccaagtg 2520

ggactcgaga gactggtgtc tctgtccggc atcaccgacg tgaaccattg ggcccagcct 2580

aagccatctc ctagcaaagg caccggcatg aacagaccca tcggcctgag agttggcaaa 2640

ggcgtgtggg ccagactgat cctggccatg acactgaccc tgaacggcat ggccaagaag 2700

tcccagcagc ctctgcaggt cgtgctgaga ctgggaaacc tgcctccatt cttcagaggc 2760

agcgaggtgc cctttcctag accttgtcgg accaagcctg cctgcacaag caaggcctgg 2820

actcccagaa aactcgtggg gagagccgtg cggagaaagg gatctccact ctgggattgc 2880

tccaccacaa caggcacaca ccacggatgc ctgtgggaga tcccatctgt gaccctgctg 2940

gcccatccta gaaccgcctc tatcaccctg cctaccacaa gcctgaaggg ctccagaacc 3000

agctgctgcc ctagatctca ccccagactg cctaggacca aatggcccct gcagtgtctg 3060

ctgcacagcc tgggaacaac cggaacactc gtttgggaaa ggggcgtcct gcagatgaga 3120

tccctcgaag cccattgtcc actgcagacc ttccatctgc accactgcag gacccagcct 3180

agctcttgtc ctcctgcctg gattctgtac caaggacagt ggagcggagc cgtcgtggga 3240

cttagacctg tggctcacca tctgcctaga cctatccagg gcctgcactg tccacaggca 3300

cctcatcaac ctgctctgag ctggggaaaa tggcccgtgg gaatgacagg ccctgccaga 3360

tgttgcagat ggccgacg gtgccagaag aaggttccat cagcctctcc atggggcctc 3420

3480

agcaccctgg gcaatttctg gatgaagctg ctccagctgc ctgtcgtgat caacctgaag 3540

agactgcaga agttcaacct gcacctgtgc tactaccaca gccagagcaa cagaaacaag 3600

agccgccaga tggaatccct gggcatgaag ttgcagtccc agaaatccca gcaccactgt 3660

3720

gctcaagaga aggccacctc tcggattctg aacctgcaag tgctcaagaa gatcctgcgg 3780

agctttatga agctgtacca ctctctggtc atgtgcctgc ggctgaggac caagctggaa 3840

aaggctctga gcgccctgtt catctggccc cagcacagct acaagcccct gttctttcac 3900

ggcgacctgt gcgagtgggg aggccaggct catagacagc ggaactggct gaaaaactgg 3960

aagcggttca agcttaaaaa gaagtacatc cagaagaaga agcagaagtc taacaagatc 4020

atctgcaagg gccaccgcat caccatcatc tgtatcaaga atatgttcct gcagcgcaag 4080

ggcttcctga acctgagcca cgtggaaaag ttctgtagag gcggccctag cgccagcgat 4140

agatgctgtt cttgtagccc cagcggcttc agtgccggca gaggaagatg tcctgttcag 4200

ggctgtctga ggccccacag agttcaactg ctgagaagat ggggccctgg ctctcctgct 4260

ggacagaggc tgtctaaggg ctttcagctg ctccgttggt ggggaccagg atctcctgct 4320

cctgagccta gaaagggccc atttccacca cctgatcctc catggcctgt gaccgctgtg 4380

acagtgatgg ctggatctgt gcctagtgct cagtctgtgg acgccctgga atctcctgga 4440

cctctggctc tcgaaggacc tagcagccct agaaacctgc tttggagaga gatgagcatc 4500

tttctgcccg gcatcttcac cgccaagaaa agccccacca gcttttgctg caatatcctg 4560

aacagcctgc caagctctat ggaaatcgcc gtgctgcaag aggacagcaa aaagatgacc 4620

cagaagggcc aagccttcgg ctccctgtgt cagcttgtgt ccgctgactt ctctgcccct 4680

caagtgaccc ggctgcggag cctgaatcat ctgctgatca cctacacacc ccacatctgc 4740

agacacaccc tgctgacccc tgacaccaga tggatcttta gcaatcagct gcagacagtc 4800

cagatgctct gtctgcctcc actgaagcag ggcgagagaa tcatttggcc cctcggcggc 4860

tttcagatcc ccaacttctt ttggagcctc ggcatcggac acactggcgc ctggtggcat 4920

actggaaggc ggagaagaga atggggcggc agcaccgtgt ctagaagccc tctgtggatc 4980

agctccatga tctacctgag aggcatggcc ttcgccagcg acagcagcat cagaaccacc 5040

agagtgtcct ggtgcagaag atgcggcgcc aaaagcgctg ccgccagctc tattgaagct 5100

ctgaccgctc tgtttacctg ccggaccgcc agcatcagcg tgaacatcag caccctgatg 5160

tccatcctgg aagcacttaa gaagaaaccc agagacatct gccgcatcag ctgccggtgt 5220

agacgcgagt acagagtgac tatgggctac ccctacgatg tccctgacta tgcgtcctaa 5280

aggcgcgcct agcggccgc 5299

<210> 1219

<211> 37184

<212> DNA

<213> Great Ape Adenovirus

<400> 1219

catcatcaat aatatacctt attttggatt gaggccaata tgataatgag gtgggcgggg 60

cgaggcgggg cgggtgacgt aggacgcgcg agtagggttg ggaggtgtgg cggaagtgtg 120

gcatttgcaa gtgggaggag ctgacatgca atcttccgtc gcggaaaatg tgacgttttt 180

gatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gcttttcacc ggatatcgta 240

gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacggggga 300

agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360

actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420

gggtcaaagt ctccgttttt attgtcgccg tcatctgacg cggagggtat ttaaacccgc 480

tgcgctccta aagaggccac tcttgagtgc cagcgagaag agttttctcc tccgctccgt 540

ttcggcgatc gaaaaatgag acatttagcc tgcactccgg gtcttttgtc cggccgggcg 600

gcgtccgagc ttttggacgc tttgctcaat gaggttctga gcgatgattt tccgtctact 660

acccacttta gcccacctac tcttcacgaa ctgtacgatc tggatgtact ggtggatgtg 720

aacgatccca acgaggaggc ggtttctacg ttttttcccg agtctgcgct tttggctgcc 780

caggagggat ttgacctaca cactccgccg ctgcctattt tagagtctcc gctgccggag 840

cccagtggta taccttatat gcctgaactg cttcccgaag tggtagacct gacctgccac 900

gagccgggct ttccgcccag cgacgatgag ggtgagcctt ttgctttaga ctatgctgag 960

atacctgggc tcggttgcag gtcttgtgca tatcatcaga gggttaccgg agaccccgag 1020

gttaagtgtt cgctgtgcta tatgaggctg acctcttcct ttatctacag taagtttttt 1080

tgtgtaggtg ggctttttgg gtaggtgggt tttgtggcag gacaggtgta aatgttgctt 1140

gtgttttttg tacctgcagg tccggtgtcc gagccagacc cggagcccga ccgcgatccc 1200

gagccggatc ccgagcctcc tcgcaggcca aggaaattac cttccatttt gtgcaagcct 1260

aagacacctg tgaggaccag cgaggcggac agcactgact ctggcacttc tacctctcct 1320

cctgaaattc acccagtggt tcctctggggt atacatagac ctgttgctgt tagagtttgc 1380

gggcgacgcc ctgcagtaga gtgcattgag gacttgctta acgatcccga gggacctttg 1440

gacttgagca ttaaacgccc taggcaataa accccaccta agtaataaac cccacctaag 1500

1560

tcatgtgtat aataaaagtg agtgtggtca taggtctctt gtttgtctgg gcggggttta 1620

agggtatata agtttctcgg ggctaaactt ggttacactt gaccccaatg gaggcgtggg 1680

ggtgcttgga ggagtttgcg gacgtgcgcc gtttgctgga cgagagctct agcaatacct 1740

atagtatttg gaggtatctg tggggctcta ctcaggccaa gttggtcttc agaattaagc 1800

1860

tgaatctggg ccaccaggct atcttccagg aaaaggttct ctcgactttg gatttttcca 1920

ctcccgggcg caccgccgct tgtgtggctt ttgtgtcttt tgtgcaagat aaatggagcg 1980

gggagaccca cctgagtcac ggctacgtgc tggatttcat ggcgatggct ctttggaggg 2040

cttacaacaa atggaagatt cagaaggaac tgtacggttc cgccctacgt cgtccacttc 2100

tgcagcggca ggggctgatg tttcccgacc atcgccagca tcagaatctg gaagacgagc 2160

gagcggagaa gatcagcttg agagccggcc tggaccctcc tcaggaggaa tgaatctccc 2220

gcaggtggtt gagctgtttc ccgaactgag acgggtcctg actatcaggg aggatggtca 2280

gtttgtgaag aagctgaaga gggatcgggg tgagggagat gatgaggcgg ctagcaattt 2340

agcttttagt ctgataactc gccaccgacc ggaatgtatt acctatcagc agattaagga 2400

gagttgtgcc aacgagctgg atcttttggg tcagaagtat agcatagaac agcttaccac 2460

ttactggctt cagcccgggg atgattggga agaggcgatt agggtgtatg caaaggtggc 2520

cctgcggccc gattgcaagt ataagattac taagttggtt aatattagaa actgctgcta 2580

tatttctgga aacggggccg aagtggagat agatactgag gacagggtgg ctattaggtg 2640

ttgcatgata aacatgtggc ccgggatact ggggatggat ggggtgatat ttatgaatgt 2700

gaggttcacg ggccccaact ttaatggtac ggtgttcatg ggcaacacca acttgctcct 2760

2820

ggttcgaggt tgttcctttt atagctgttg gaaggcggtg gtgtgtcgcc ctaaaagcag 2880

gggttctgtg aagaaatgct tgtttgaaag gtgcacccta ggtatccttt ctgagggcaa 2940

ctccagggtg cgccataatg tggcttcgaa ctgcggttgc ttcatgcaag tgaagggggt 3000

gagcgttatc aagcataact cggtctgtgg aaactgcgag gatcgcgcct ctcagatgct 3060

gacctgcttt gatggcaact gtcacctgtt gaagaccatt catataagca gtcaccccag 3120

aaaggcctgg cccgtgtttg agcataacat tctgacccgc tgttccttgc atctgggggt 3180

caggaggggt atgttcctgc cttaccagtg taactttagc cacactaaaa tcctgctgga 3240

acccgagtgc atgactaagg tcagcctgaa tggtgtgttt gatgtgagtc tgaagatttg 3300

gaaggtgctg aggtatgatg agaccaggac caggtgccga ccctgcgagt gcggcggcaa 3360

gcacatgaga aatcagcctg tgatgttgga tgtgaccgag gagcttaggc ctgaccatct 3420

ggtgctggcc tgcaccagggg ccgagtttgg gtctagcgat gaggataccg attgaggtgg 3480

gtaaggtggg cgtggctagc aggtgggcg tgtataaatt gggggtctaa ggggtctctc 3540

tgtttgtctt gcaacagccg ccgccatgag cgacaccggc aacagctttg atggaagcat 3600

3660

gggttccaac gtggatggac gtcccgttct gccttcaaat tcgtctacta tggcctacgc 3720

gaccgtggga ggaactccgc tggacgccgc gacctccgcc gccgcctccg ccgccgccgc 3780

gaccgcgcgc agcatggcta cggaccttta cagctctttg gtggcgagca gcgcggcctc 3840

tcgcgcgtct gctcgggatg agaaactgac tgctctgctg cttaaactgg aagacttgac 3900

ccgggagctg ggtcaactga cccagcaggt ttccagcttg cgtgagagca gccttgcctc 3960

cccctaatgg cccataatat aaataaaagc cagtctgttt ggattaagca agtgtatgtt 4020

ctttatttaa ctctccgcgc gcggtaagcc cgggaccagc ggtctcggtc gtttagggtg 4080

cggtggattt tttccaacac gtggtacagg tggctctgga tgtttagata catgggcatg 4140

agtccatccc tggggtggag gtagcaccac tgcagagctt cgtgctcggg ggtggtgttg 4200

tatatgatcc agtcgtagca ggagcgctgg gcgtggtgct gaaaaatgtc cttaagcaag 4260

aggcttatag ctagggggag gcccttggtg taagtgttta caaatctgct tagctggggag 4320

gggtgcatcc ggggggatat gatgtgcatc ttggactgga tttttaggtt ggctatgttc 4380

ccgcccagat cccttctggg attcatgttg tgcaggacca ccagcacggt atatccagtg 4440

cacttgggaa atttatcgtg gagcttagac gggaatgcat ggaagaactt ggagacgccc 4500

ttgtggcctc ccagattttc catacattcg tccatgatga tggcaatggg cccgtgggaa 4560

gctgcctgag caaaaacgtt tctggcatcg ctcacatcgt agttatgttc cagggtgagg 4620

tcatcatagg acatctttac gaatcggggg cgaagggtcc cggactgggg gatgatggta 4680

ccctcgggcc ccggggcgta gttcccctca cagatctgca tctcccaggc tttcatttca 4740

gagggaggga tcatatccac ctgcggggcg atgaaaaaga cagtttctgg cgcaggggag 4800

attaactggg atgagagcag gtttctgagc agctgtgact ttccacagcc ggtgggccca 4860

tatatcacgc ctatcaccgg ctgcagctgg tagttaagag agctgcagct gccgtcctcc 4920

cggagcaggg gggccacctc gttgagcata tccctgacgt ggatgttctc cctgaccagt 4980

tccgccagaa ggcgctcgcc gcccagcgaa agcagctctt gcaaggaagc aaaatttttc 5040

agcggtttca ggccatcggc cgtgggcatg tttttcagcg tctgggtcag cagctccagc 5100

ctgtcccaga gctcggtgat gtgctctacg gcatctcgat ccagcagatc tcctcgtttc 5160

gcgggttggg gcggctttcg ctgtagggca ccagccgatg ggcgtccagc ggggccagag 5220

tcatgtcctt ccatgggcgc agggtcctcg tcagggtggt ctgggtcacg gtgaaggggt 5280

gcgctccggg ttgggcactg gccagggtgc gcttgaggct ggttctgctg gtgctgaatc 5340

gctgccgctc ttcgccctgc gcgtcggcca ggtagcattt gaccatggtc tcgtagtcga 5400

gaccctcggc ggcgtgcccc ttggcgcgga gctttccctt ggaggtggcg ccgcacgagg 5460

ggcactgcag gctcttcagg gcgtagagct tgggagcgag aaacacggac tctggggagt 5520

aggcgtccgc gccgcaggcc gagcagaccg tctcgcattc caccagccaa gtgagttccg 5580

ggcggtcagg gtcaaaaacc aggttgcccc catgcttttt gatgcgtttc ttaccttggc 5640

tctccatgag gcggtgtccc ttctcggtga cgaagaggct gtccgtgtcc ccgtagaccg 5700

acttcagggg cctgtcttcc agcggagtgc ctctgtcctc ctcgtagaga aactctgacc 5760

actctgagac gaaggcccgc gtccaggcca ggacgaagga ggccacgtgg gaggggtagc 5820

ggtcgttgtc cactagcggg tccaccttct ccagggtgtg caggcacatg tccccctcct 5880

ccgcgtccag aaaagtgatt ggcttgtagg tgtaggacac gtgaccgggg gttcccaacg 5940

ggggggtata aaagggggtg ggtgcccttt catcttcact ctcttccgca tcgctgtctg 6000

cgagagccag ctgctggggt aagtattccc tctcgaaggc gggcatgacc tcagcgctca 6060

ggttgtcagt ttctaaaaat gaggaggatt tgatgttcac ctgtccggag gtgatacctt 6120

tgagggtacc tgggtccatc tggtcagaaa acactatttt tttgttatca agcttggtgg 6180

cgaatgaccc gtagagggcg ttggagagca gcttggcgat ggagcgcagg gtctggtttt 6240

tgtcgcggtc ggctcgctcc ttggccgcga tgttgagttg cacgtactcg cgggccacgc 6300

acttccactc ggggaacacg gtggtgcgct cgtctgggat caggcgcacc ctccagccgc 6360

ggttgtgcag ggtgaccatg tcgacgctgg tggcgacctc accgcgcaga cgctcgttgg 6420

tccagcagag gcggccgccc ttgcgcgagc agaagggggg tagggggtcc agctggtcct 6480

cgtttggggg gtccgcgtcg atggtaaaga ccccggggag caggcgcggg tcaaagtagt 6540

cgatcttgca agcttgcatg tccagagccc gctgccattc gcgggcggcg agcgcgcgct 6600

6660

cgcagatgtc atacacgtac aggggttccc tgaggatacc gaggtaggtg gggtagcagc 6720

gccccccgcg gatgctggcg cgcacgtagt catagagctc gtgggagggg gccagcatgt 6780

tgggcccgag gttggtgcgc tgggggcgct cggcgcggaa gacgatctgc ctgaagatgg 6840

cgtgggagtt ggaggagatg gtgggccgct ggaagacgtt gaagcttgct tcttgcaagc 6900

ccacggagtc cctgacgaag gaggcgtagg actcgcgcag cttgtgcacc agctcggcgg 6960

tgacctggac gtcgagcgca cagtagtcga gggtctcgcg gatgatgtca tacctatcct 7020

cccccttctt tttccacagc tcgcggttga ggacgaactc ttcgcggtct ttccagtact 7080

cttggagggg aaacccgtcc gtgtccgaac ggtaagagcc tagcatgtag aactggttga 7140

cggcctggta ggggcagcag cccttctcca cgggcagcgc gtaggcctgc gccgccttgc 7200

ggagggaggt gtgggtgagg gcgaaagtgt ccctgaccat gactttgagg tattgatgtc 7260

tgaagtctgt gtcatcgcag ccgccctgtt cccacagggt gtagtccgtg cgctttttgg 7320

agcgcggggtt gggcagggag aaggtgaggt cattgaagag gatcttcccc gctcgaggca 7380

tgaagtttct ggtgatgcga aagggccctg ggaccgagga gcggttgttg atgacctggg 7440

cggccaggac gatctcgtca aagccgttta tgttgtgtcc cacgatgtag agctccagga 7500

agcggggctg gcccttgatg gaggggagct ttttaagttc ctcgtaggta agctcctcgg 7560

gcgattccag gccgtgctcc tccagggccc agtcttgcaa gtgagggttg gccgccagga 7620

aggatcgcca gaggtcgcgg gccatgaggg tctgcaggcg gtcgcggaag gttctgaact 7680

gccgccccac ggccattttt tcggggggtga tgcagtagaa ggtgagggggg tctttctccc 7740

aggggtccca tctgagctct cgggcgaggt cgcgcgcggc agcgaccaga gcctcgtcgc 7800

cccccagttt catgaccagc atgaagggca cgagttgctt gccaaaggct cccatccaag 7860

tgtaggtttc tacatcgtag gtgacaaaga ggcgctccgt gcgaggatga gagccgattg 7920

ggaagaactg gatctcccgc caccagttgg aggattggct gttgatgtgg tgaaagtaga 7980

agtcccgtct gcgggccgag cactcgtgct ggcttttgta aaagcgaccg cagtactggc 8040

agcgctgcac gggttgtata tcttgcacga ggtgaacctg gcgacctctg acgaggaagc 8100

8160

ttgtctggcc gccagcatct gtctcctgga gggcgatggt ggaacagacc accacgccgc 8220

gagagccgca ggtccagatc tcggcgctcg gcgggcggag tttgatgacg acatcgcgca 8280

cattggagct gtccatggtc tccagctccc gcggcggcag gtcagccggg agttcctgga 8340

ggttcacctc gcagagacgg gtcaaggcgc ggacagtgtt gagatggtat ctgatttcaa 8400

ggggcatgtt ggaggcggag tcgatggctt gcaggaggcc gcagccccgg ggggccacga 8460

tggttccccg cggggcgcga ggggaggcgg aagctggggg tgtgttcaga agcggtgacg 8520

cgggcgggcc cccgggaggta gggggggttc cggccccaca ggcatgggcg gcaggggcac 8580

gtcttcgccg cgcgcgggca ggggctggtg ctggctccga agagcgcttg cgtgcgcgac 8640

gacgcgacgg ttggtgtcct gtatctggcg cctctgagtg aagaccacgg gtcccgtgac 8700

cttgaacctg aaagagagtt cgacagaatc aatctcggca tcgttgacag cggcctggcg 8760

caggatctcc tgcacgtcgc ccgagttgtc ctggtaggcg atttctgcca tgaactgctc 8820

gatctcttcc tcctggagat ctcctcgtcc ggcgcgctcc acggtggccg ccaggtcgtt 8880

ggagatgcga cccatgagct gcgagaaggc gttgagtccg ccctcgttcc agacccggct 8940

gtagaccacg cccccctcgg cgtcgcgggc gcgcatgacc acctgggcca ggttgagctc 9000

cacgtgtcgc gtgaagacgg cgtagttgcg caggcgctgg aaaaggtagt tcagggtggt 9060

ggcggtgtgc tcggcgacga agaagtacat gacccagcgc cgcaacgtgg attcattgat 9120

gtcccccaag gcctccaggc gctccatggc ctcgtagaag tccacggcga agttgaaaaa 9180

ctgggagttg cgagcggaca cggtcaactc ctcctccaga agacggatga gctcggcgac 9240

agtgtcgcgc acctcgcgct cgaaggccac ggggggcgct tcttcctctt ccacctcttc 9300

ttccatgatt gcttcttctt cttcctcagc cgggacggga gggggcggcg gcgggggagg 9360

ggcgcggcgg cggcggcggc gcaccgggag gcggtcgatg aagcgctcga tcatctcccc 9420

ccgcatgcgg cgcatggtct cggtgacggc gcggccgttc tcccgggggc gcagctcgaa 9480

gacgccgcct ctcatttcgc cgcggggcgg gcggccgtga ggtagcgaga cggcgctgac 9540

tatgcatctt aacaattgct gtgtaggtac gccgccaagg gacctgattg agtccagatc 9600

caccggatcc gaaaaccttt ggaggaaagc gtctatccag tcgcagtcgc aaggtaggct 9660

gagcaccgtg gcgggcgggg gcgggtcggg agagttcctg gcggagatgc tgctgatgat 9720

gtaattaaag taggcggtct tgagaaggcg gatggtggac aggagcacca tgtctttgggg 9780

tccggcctgt tggatgcgga ggcggtcggc catgccccag gcctcgttct gacaccggcg 9840

caggtctttg tagtaatctt gcatgagtct ttccaccggc acttcttctc cttcctcttc 9900

ttcatctcgc cggtggtttc tcgcgccgcc catgcgcgtg accccaaagc ccctgagcgg 9960

ctgcagcagg gccaggtcgg cgaccacgcg ctcggccaag atggcctgct gtacctgagt 10020

gagggtcctc tcgaagtcat ccatgtccac gaagcggtgg taggcacccg tgttgatggt 10080

gtaggtgcag ttggccatga cggaccagtt gacggtctgg tgtcccggct gcgagagctc 10140

cgtgtaccgc aggcgcgaga aggcgcggga atcgaacacg tagtcgttgc aagtccgcac 10200

cagatactgg tagcccacca ggaagtgcgg cggaggttgg cgatagaggg gccagcgctg 10260

ggtggcgggg gcgccgggcg ccaggtcttc cagcatgagg cggtggtatc cgtagatgta 10320

10380

gttccagatg tttcgcaggg gcgagaagtg ttccatggtc ggcacgctct ggccggtgag 10440

10500

10560

agctgagctc agccggctga agccgcagct aacgtggtat tggcagtccc gtctcgaccc 10620

10680

10740

tggagaaaca atcgccaggg ttgcgttgcg gcgtaccccg gttcgagccc ctatggcggc 10800

ttggatcggc cggaaccgcg gctaacgtgg gctgtggcag ccccgtcctc aggaccccgc 10860

cagccgactt ctccagttac gggagcgagc cccttttgtt tttttatttt ttagatgcat 10920

cccgtgctgc ggcagatgcg cccctcgccc cggcccgatc agcagcagca acagcaggca 10980

tgcagacccc cctctcctct ccccgccccg gtcaccacgg ccgcggcggc cgtgtccggt 11040

gcggggggcg cgctggagtc agatgagcca ccgcggcggc gacctaggca gtatctggac 11100

ttggaagagg gcgagggact ggcgcggctg ggggcgagct ctccagagcg ccacccgcgg 11160

gtgcagttga aaagggacgc gcgtgaggcg tacctgccgc ggcaaaacct gtttcgcgac 11220

11280

cgccgcggct tggacagaca gcgcctgctg cgcgaggagg actttgagcc cgacacgcag 11340

acgggcatca gccccgcgcg cgcgcacgtg gccgcggccg acctggtgac cgcctacgag 11400

cagacggtga accaggagcg caacttccaa aaaagcttca acaaccacgt gcgcacgctg 11460

gtggcgcgcg aggaggtgac cctgggtctc atgcatctgt gggacctggt ggaggcgatc 11520

11580

11640

11700

gccgagaagg tggcggccat taactattct atgctgagcc tgggcaagtt ctacgctcgc 11760

aagatctaca agacccccta cgtgcccata gacaaggagg tgaagataga cagcttctac 11820

atgcgcatgg cgctgaaggt gctaaccctg agcgacgacc tgggagtgta ccgcaacgag 11880

cgcatccaca aggccgtgag cgccagccgg cggcgcgagc tgagcgaccg cgaactgatg 11940

cacagtctgc agcgcgcgct gaccggcgcg ggcgaggggcg acagggaggt cgagtcctac 12000

tttgacatgg gggccgacct gcactggcag ccgagccgcc gcgccctgga agcggcgggg 12060

gcgtacggcg gccccctggc ggccgatgac gaggaagagg aggactatga gctagaggag 12120

ggcgagtacc tggaggactg acctggctgg tggtgttttg gtatagatgc aagatccgaa 12180

cgtggcggac ccggcggtcc gggcggcgct gcagagccag ccgtccggca ttaactcctc 12240

tgacgactgg gccgcggcca tgggtcgcat catggccctg accgcgcgca accccgaggc 12300

cttcaggcag cagcctcagg ctaaccggct ggcggccatc ttggaagcgg tagtgcccgc 12360

gcgctccaac cccacccacg agaaggtgct ggccatagtc aacgcgctgg cggagagcag 12420

ggccatccgg gcagacgagg ccggactggt gtacgatgcg ctgctgcagc gggtggcgcg 12480

gtacaacagc ggcaacgtgc agaccaacct ggaccgcctg gtgacggacg tgcgcgaggc 12540

cgtggcgcag cgcgagcgct tgcatcagga cggcaacctg ggctcgctgg tggcgctaaa 12600

12660

cttgagcgcg ctgcggctga tggtgaccga ggtccctcag agcgaggtgt accagtcggg 12720

12780

tttcaagaac ctgcgggggc tgtggggagt gaaggcgccc accggcgacc gggctacggt 12840

gtccagcctg ctaaccccca actcgcgcct gctgctgctg ctgatcgcgc ccttcacgga 12900

cagcgggagc gtctcgcggg agacctatct gggccacctg ctgacgctgt accgcgaggc 12960

catcgggcag gcgcaggtgg acgagcacac cttccaggag atcaccagcg tgagccacgc 13020

gctggggcag gaggacacgg gcagcctgca ggcgaccctg aactacctgc tgaccaacag 13080

gcggcagaag attcccacgc tgcacagcct gacccaggag gaggagcgca tcttgcgcta 13140

cgtgcagcag agcgtgagcc tgaacctgat gcgcgacggc gtgacgccca gcgtggcgct 13200

ggacatgacc gcgcgcaaca tggaaccggg catgtacgct tcccagcggc cgttcatcaa 13260

ccgcctgatg gactacttgc atcgggcggc ggccgtgaac cccgagtact tcaccaatgc 13320

cattctgaat ccccactgga tgccccctcc gggtttctac aacggggact tcgaggtgcc 13380

tgaggtcaac gatgggttcc tctgggatga catggatgac agtgtgttct cccccaaccc 13440

gctgcgcgcc gcgtctctgc gattgaagga gggctctgac agggaaggac caaggagtct 13500

ggcctcctcc ctggctctgg gggcggtggg cgccacgggc gcggcggcgc ggggcagcag 13560

ccccttcccc agcctggcgg actctctgaa tagcgggcgg gtgagcaggc cccgcttgct 13620

aggcgaggag gagtatctga acaactccct gctgcagccc gtgagggaca aaaacgctca 13680

13740

gacgtatgcg caggagtaca aggagtggga ggaccgccag ccgcggcccc tgccgccccc 13800

tagacagcgc tggcagcggc gcgcgtccaa ccgccgctgg aggcaggggc ccgaggacga 13860

tgatgactct gcagatgaca gcagcgtgtt ggacctgggc gggagcggga accccttttc 13920

gcacctgcgc cccacgcctgg gcaagatgtt ttaaaagaga aaaataaaaa ctcaccaagg 13980

ccatggcgac gagcgttggt ttttgttcc cttccttagt atgcggcgcg cggcgatgtt 14040

cgaggagggg cctcccccct cttacgagag cgcgatggga atttctcctg cggcgcccct 14100

gcagcctccc tacgtgcctc ctcggtacct gcaacctaca ggggggagaa atagcatctg 14160

ttactctgag ctgcagcccc tgtacgatac caccagactg tacctggtgg acaacaagtc 14220

cgcggacgtg gcctccctga actaccagaa cgaccacagc gattttttga ccacggtgat 14280

ccaaaacaac gacttcaccc caaccgaggc cagtacccag accataaacc tggacaacag 14340

gtcgaactgg ggcggcgacc tgaagactat cctgcacacc aatatgccca acgtgaacga 14400

gttcatgttc accaactctt ttaaggcgcg ggtgatggtg gcgcgcgagc agggggaggc 14460

14520

14580

gaaggagagc gatatcgggg tcaagtttga caccagaaac ttccgtctgg gctgggaccc 14640

14700

14760

tcgcaagcgg caacctttcc aggagggttt caagatcacc tatgaggatc tggagggggg 14820

14880

cgctggcgac agcggcgaga gtggcgagga gcaagccggc ggcggcggca gcgcgtcggt 14940

agaaaacgaa agtactcccg cagtggcggc ggacgctgcg gaggtcgagc cggaggccat 15000

gcagcaggac gcagaggagg gcgcgcagga ggacatgaac aatggggaga tcaggggcga 15060

cactttcgcc acccggggcg aagaaaaaga ggcagaggcg gcggcggcga cggcggaagc 15120

15180

acgtagggggt gacacgtttg ccacccgggg cgaagagaag gcggcgggagg cagaagccgc 15240

ggctgaggag gcggctgcgg ctgcggccaa ggctgaggct gcggctgagg ctaaggtcga 15300

agccgatgtt gcggttgagg ctcaggctga ggaggaggcg gcggctgaag cagttaagga 15360

aaaggcccag gcagagcagg aagagaaaaa acctgtcatt caacctctaa aagaagatag 15420

15480

cctggcttac aactacggcg acccggtcaa gggggtgcgc tcgtggaccc tgctctgcac 15540

gccggacgtc acctgcggct ccgagcagat gtactggtcg ctgccaaaca tgatgcaaga 15600

cccggtgacc ttccgttcca cgcggcaggt tagcaacttt ccggtggtgg gcgccgaact 15660

gctgccagta cactccaaga gtttttacaa cgagcaggcc gtctactccc agctgatccg 15720

ccaggccacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg 15780

cccgccggcc cccaccatca ccaccgtcag tgaaaacgtt cctgccctca cagatcacgg 15840

gacgctaccg ctgcgcaaca gcatctcagg agtccagcga gtgaccatta ctgacgccag 15900

acgccggacc tgcccctacg tttacaaggc cttgggcata gtctcgccgc gcgtcctctc 15960

cagtcgcact ttttaaaaca catccaccca cacgctccaa aatcatgtcc gtactcatct 16020

cgcccagcaa caacaccggc tgggggctgc gcgcacccag caagatgttt ggaggggcaa 16080

ggaagcgctc cgaccagcac cccgtgcgcg tgcgcggcca ctaccgcgcg ccctggggtg 16140

16200

agcaggcgcg ccactacaca cccggcgcgc cgaccgcctc cgccgtgtcc accgtggacc 16260

aggcgatcga aagcgtggta cagggggcgc ggcactatgc caaccttaaa agtcgccgcc 16320

gccgcgtggc gcgccgccat cgccggagac cccgggctac tgccgccgcg cgccttacca 16380

aggctctgct caagcgcgcc aggcgaactg gccaccgggc cgccatgagg gccgcacggc 16440

gggctgccgc tgccgcgagc gccgtggccc cgcgggcacg aaggcgcgcg gccgctgccg 16500

16560

actcggtgag cggcacacgt gtgcccgtgc gctttcgccc cccacggaat tagcacaaga 16620

caacatacac actgagtctc ctgctgttgt gtatcccagc ggcgaccgtc agcagcggcg 16680

acatgtccaa gcgcaaaatt aaagaagaga tgctccaggt catcgcgccg gagatctatg 16740

ggcccccgaa gaaggaggag gaggattaca agccccgcaa gctaaagcgg gtcaaaaaga 16800

aaaagaaaga tgatgacgtt gacgaggcgg tggagtttgt ccgccgcatg gcgcccaggc 16860

gccctgtgca gtggaagggt cggcgcgtgc agcgagtcct gcgccccggc accgcggtgg 16920

tctttacgcc cggcgagcgt tccacgcgca ctttcaagcg ggtgtacgat gaggtgtacg 16980

gcgacgagga tctgttggag caggccaacc atcgatttgg ggagtttgca tatgggaaac 17040

ggcctcgcga gagtctaaaa gaggacctgc tggcgctacc gctggacgag ggcaatccca 17100

ccccgagtct gaagccggtg accctgcaac aggtgctgcc tttgagcgcg cccagcgagc 17160

agaagcgagg gttaaagcgc gagggcgggg acctggcacc caccgtgcag ttgatggtgc 17220

ccaagcggca gaagctggag gacgtgctgg agaaaatgaa agtagagccc gggatccagc 17280

ccgagatcaa ggtccgccct atcaagcagg tggcgcccgg cgtgggagtc cagaccgtgg 17340

acgttaggat tcccacggag gagatggaaa cccaaaccgc cactccctct tcggcagcaa 17400

gcgccaccac cggcgccgct tcggtagagg tgcagacgga cccctggcta cccgccgcca 17460

ctatcgccgt cgccgccgcc ccccgttcgc gcggacgcaa gagaaattat cgcggcca 17520

gcgcgcttat gccccagtat gcgctgcatc catccatcgc gcccaccccc ggctaccgcg 17580

ggtactcgta ccgcccgcgc agatcagccg gcactcgcgg ccgccgccgc cgtgcgacca 17640

caaccagccg ccgccgtcgc cgccgccgcc agccagtgct gacccccgtg tctgtaagga 17700

17760

tttaaagccg gtctctgtat ggttcttgca gatatggccc tcacttgtcg ccttcgcttc 17820

ccggtgccgg gataccgagg aagaactcac cgccgcaggg gcatggcggg cagcggtctc 17880

cgcggcggcc gtcgccatcg ccggcgcgca aagagcaggc gcatgcgcgg cggtgtgttg 17940

cccctgctgg tcccgctact cgccgcggcg atcggcgccg tgcccgggat cgcctccgtg 18000

gccctgcagg cgtcccagaa acattgactc ttgcaacctt gcaagcttgc atttttggag 18060

gaaaaaataa aaaagtctag actctcacgc tcgcttggtc ctgtgactat tttgtagaaa 18120

18180

gggagactgg acagatatcg gcaccagcaa tatgagcggt ggcgccttca gctggggcag 18240

tctgtggagc ggccttaaaa attttggttc caccattaag aactatggca acaaagcgtg 18300

gaacagcagc acgggtcaga tgctgagaga caagttgaaa gagcagaact tccaggagaa 18360

ggtggcgcag ggcctggcct ctggcatcag cggggtggtg gacatagcta accaggccgt 18420

gcagaaaaag ataaacagtc atctggaccc ccgccctcag gtggaggaaa cgcctccagc 18480

catggagacg gtgtctcccg agggcaaagg cgaaaagcgc ccgcggcccg acagggaaga 18540

gaccctggtg tcacacaccg aggagccgcc ctcttacgag gaggcagtca aggccggcct 18600

gcccaccact cgccccatag ctcccatggc caccggtgtg gtgggtcaca ggcaacacac 18660

ccccgcaaca ctagatctgc ccccgccgtc cgagccgact cgccagccaa aggcggtgac 18720

ggtgtccgct ccctccactt ccgccgccaa cagagtgcct ctgcgccgcg ctgcgagcgg 18780

cccccgggcc tcgcgagtca gcggcaactg gcagagcaca ctgaacagca tcgtgggcct 18840

gggagtgagg agtgtgaagc gccgccgttg ctactgaatg agcaagctag ctaacgtgtt 18900

18960 gtatgtgtgt atgcgtccta tgtcgccgcc

gcactccagc gaatttcaag atggcgaccc catcgatgat gcctcagtgg tcgtacatgc 19020

acatctcggg ccaggacgct tcggagtacc tgagccccgg gctggtgcag ttcgcccgcg 19080

cccagacac ctacttcaac atgagtaaca agttcaggaa ccccactgtg gcgcccaccc 19140

acgatgtgac cacggaccgg tcgcagcgcc tgacgctgcg gttcatcccc gtggatcggg 19200

aggaccgc ttactcttac aaggcgcggt tcacgctggc cgtgggcgac aaccgcgtgc 19260

tggacatggc ctccacttac tttgacatcc ggggggtgct ggacaggggc cccactttta 19320

agccctactc gggcactgcc tacaaccccc tggcccccaa gggcgccccc aattcttgtg 19380

agtgggaaca agaggaaaat caggtggtcg ctgcagatga tgaacttgaa gatgaagaag 19440

19500

ctggcgaaaa gatttccaag gatggtatcc aaataggtac tgaagtcgta ggagatacat 19560

ctaaggacac ttttgcagat aaaacattcc aacccgaacc tcagataggc gagtctcagt 19620

ggaacgaggc tgatgccaca gcagcaggag gtagagtttt gaaaaagact acccctatga 19680

gaccttgcta tggatcctat gccaggccta ccaatgccaa cgggggtcaa ggaattatgg 19740

ttgccaatga acaaggagtg ttggagtcta aagtagaaat gcaatttttc tctaacacca 19800

caacccttaa tgcgcgggat ggaaccggca atcccgaacc aaaggtggtg ttgtacagcg 19860

aagatgtcca cttggaatct cccgatactc atctgtctta caagcccaaa aaggatgatg 19920

ttaatgccaa aatcatgttg ggtcagcaag ccatgcccaa cagacccaac ctcattggat 19980

20040

cgggtcaggc ctctcagttg aatgctgtgg tggacttgca ggatagaaac acagaactgt 20100

catatcagct tctgcttgat tcaattgggg atagaaccag atacttctcc atgtggaacc 20160

aggcagtgga tagctatgat ccagatgtca gaattattga aaaccatggg actgaggatg 20220

aactgcccaa ctactgcttc cctttggggcg gcataggagt tactgatact tatcaaggga 20280

20340

aaataggggt gggaaacaac ttcgccatgg agatcaacat ccaggccaac ctttggagaa 20400

acttcctcta tgcaaacgtg gggctctacc tgccagacaa gctcaagtac aaccccacca 20460

acgtggacat ctctgacaac cccaacacct atgactacat gaacaagcgg gtggtggccc 20520

ctggcctggt ggactgcttt gtcaatgtgg gagccaggtg gtccctggac tacatggaca 20580

acgtcaaccc cttcaaccac caccgcaatg cgggtctgcg ctaccgctcc atgatcctgg 20640

gcaacgggcg ctatgtgccc tttcacatcc aggtacccca gaagttcttt gccatcaaga 20700

acctcctgct cctgcccggc tcctacacct acgagtggaa cttcaggaag gatgtgaaca 20760

tggtcctaca gagctctctg ggcaatgacc ttagggtgga tggggccagc atcaagtttg 20820

acagcatcac cctctatgct acatttttcc ccatggccca caacaccgcc tccacgcttg 20880

aggccatgct gagaaacgac accaacgacc agtcctttaa tgactacctc tctggggcca 20940

acatgctcta cccaatccca gccaaggcca ccaacgtgcc catctccatc ccctctcgca 21000

actgggccgc ctttagaggc tgggccttta cccgccttaa gaccaagggag accccctccc 21060

tgggctcggg ttttgatccc tactttgttt actcgggatc catcccctac ctggatggca 21120

ccttctacct caaccacact ttcaagaaga tatccatcat gtatgactcc tccgtcagct 21180

ggccgggcaa cgaccgcttg ctcaccccca atgagttcga ggtcaagcgc gccgtggacg 21240

gcgagggcta caacgtggcc cagtgcaaca tgaccaagga ctggttcctg gtgcagatgc 21300

21360

tgtactcctt cttcagaaat ttccaaccca tgagccgaca ggtggtggac gagaccaatt 21420

acaaggacta tcaagccatt ggcatcaccc accagcacaa caactcgggt ttcgtgggct 21480

acctggcgcc caccatgcgc gagggtcagg cctaccccgc caacttcccc taccccttga 21540

taggcaagac cgcggtcgac agcgtcaccc agaaaaagtt cctctgcgac cgcaccctct 21600

ggcgcatccc cttctctagc aacttcatgt ccatgggtgc gctcacggac ctgggccaaa 21660

acctgcttta tgccaactct gcccatgcgc tggacatgac ttttgaggtg gaccccatgg 21720

acgagcccac ccttctctat attgtgtttg aagtgttcga cgtggtcaga gtgcaccagc 21780

cgcaccgcgg tgtcatcgag accgtgtacc tgcgtacgcc cttctcagcc ggcaacgcca 21840

ccacctaagg agacagcgcc gccgccgcct gcatgacggg ttccaccgag caagagctca 21900

gggccattgc cagagacctg ggatgcggac cctatttttt gggcacctat gacaaacgct 21960

22020

agaccggggg cgtgcactgg ctggcctttg gctgggaccc gcgctccaaa acttgctacc 22080

tctttgaccc ctttggcttc tccgatcagc

aggggctgct gcgccgcagc gcgctcgcct cctcgcccga ccgctgcatc acccttgaga 22200

agtccaccga aaccgtgcag gggccccact cggccgcctg cggtctcttc tgttgcatgt 22260

ttttgcacgc ctttgtgcac tggcctcaga gtcccatgga ttgcaacccc accatgaact 22320

tgctaaaggg agtgcccaac gccatgctcc agagccccca ggtccagccc accctgcgcc 22380

gcaaccagga acagctttac cgcttcctgg agcgccactc cccctacttc cgcagccaca 22440

gcgcgcgcat ccggggggcc acctcttttt gccacttgca agaaaacatg caagacggaa 22500

aatgatgtac agcatgcttt taataaatgt aaagactgtg cactttaatt atacacgggc 22560

tctttctggt tatttattca acaccgccgt cgccatttag aaatcgaaag ggttctgccg 22620

22680

gaactcgggc accaccatgc ggggcagtgg ttcctcgggg aagttctcgc tccacagggt 22740

gcgggtcagc tgcagcgcgc tcaggaggtc gggagccgag atcttgaagt cgcagttggg 22800

gccggaaccc tgcgcgcgcg agttgcggta cacggggttg cagcactgga acaccagcag 22860

ggccggatta ttcacgctgg ccagcaggct ctcgtcgctg atcatgtcgc tgtccagatc 22920

ctccgcgttg ctcagggcga atggggtcat cttgcagacc tgcctgccca ggaaaggcgg 22980

gagcccaggc ttgccgttgc agtcgcagcg caggggcatt agcaggtgcc cacggcccga 23040

ctgcgcctgc gggtacaacg cgcgcatgaa ggcttcgatc tgcctaaaag ccacctgggt 23100

cttggctccc tccgaaaaga acatcccaca ggacttgctg gagaactggt tcgcgggaca 23160

gctggcatcg tgcaggcagc agcgcgcgtc agtgttggca atctgcacca cgttgcgacc 23220

ccaccggttt ttcactatct tggccttgga agcctgctcc tttagcgcgc gctggccgtt 23280

ctcgctggtc acatccatct ctatcacctg ttccttgttg atcatgtttg tcccgtgcag 23340

acactttagg tcgccctccg tctgggtgca gcggtgctcc cacagcgcgc aaccggtggg 23400

ctcccaattc ttgtgggtca cccccgcgta ggcctgcagg taggcctgca ggaagcgccc 23460

catcatggtc ataaaggtct tctggctcgt aaaggtcagc tgcaggccgc gatgctcttc 23520

gttcagccag gtcttgcaga tggcggccag cgcctcggtc tgctcgggca gcatcttaaa 23580

23640

gcccttctcc caggcggaca ccatgggcag gcttaggggg tttatcactt ccagcggcga 23700

23760

gcgcgctctt accgcctgca ccaaggggtc gtcttcaggc aagcgccgca ccgagcgctt 23820

gccgcccttg acctgcttga tcagtaccgg cgggttgctg aagcccacca tggtcagcgc 23880

cgcctgctct tcttcgtctt cgctgtctac cactatttct ggggaggggc ttctccgctc 23940

tgcggcaaag gcggcggatc gcttcttttt tttcttggga gccgccgcga tggagtccgc 24000

cacggcgacc gaggtcgagg gcgtggggct gggggtgcgc ggtaccaggg cctcgtcgcc 24060

ctcggactct tcctctgact ccaggcggcg gcggagtcgc ttctttgggg gcgcgcgcgt 24120

cagcggcggc ggagacgggg acggggacgg ggacgggacg ccctccacag ggggtggtct

tcgcgcagac ccgcggccgc gctcgggggt cttctcgcgc tggtcttggt cccgactggc 24240

cattgtatcc tcctcctcct aggcagagag acataaggag tctatcatgc aagtcgagaa 24300

24360

24420

acccctgttc gaggaagcgg ccgtggagca ggacccggggc tttgtctcgg cagaggagga 24480

tttgcaagag gaggagaata aggaggagaa gccctcagtg ccaaaagatc ataaagagca 24540

agacgagcac gacgcagacg cacaccaggg tgaagtcggg cggggggacg gagggcatgg 24600

cggcgccgac tacctagacg aaggaaacga cgtgctcttg aagcacctgc atcgtcagtg 24660

24720 cgccatcgtc tgcgacgctc tgcaggagcg cagcgaggtg

cagccgcgcc tacgagctca gcctcttttc cccccgggtg cccccccgcc gccgcgaaaa 24780

cggcacatgc gagcccaacc cgcgcctcaa cttctacccc gcctttgtgg tgcccgaggt

cctggccacc tatcacatct tctttcaaaa ttgcaagatc cccatctcgt gccgcgccaa 24900

ccgtagccgc gccgataaga tgctggccct gcgccagggc gaccacatac ctgatatcgc 24960

cgctttggaa gatgtgccaa agatcttcga gggtctgggg cgcaacgaga agcgggcagc 25020

25080 aaactctctg caacaggaaa acagcgaaaa tgagagtcac

ggagggcgac aacgcccgcc tggcggtgct caagcgcagc atcgaggtca cccactttgc 25140

ctaccccgcg ctcaacctgc cccccaaagt catgaacgcg gtcatggacg ggctgatcat 25200

gcgccgcggc cggcccctcg ctccagatgc aaacttgcat gaggagaccg aggacggtca 25260

gcccgtggtc agcgacgagc agctgacgcg ctggctggag agcgcggacc ccgccgaact

ggaggagcgg cgcaagatga tgatggccgc ggtgctggtc accgtagagc tggagtgtct 25380

gcagcgcttc ttcggtgacc ccgagatgca gagaaaggtc gaggagaccc tacactacac 25440

cttccgccag ggctacgtgc gccaggcttg caagatctcc aacgtggagc tcagcaacct 25500

ggtgtcctac ctgggcatct tgcatgaaaa ccgccttgggg cagagcgtgc tacactccac 25560

25620

cacctggcag acggccatgg gggtctggca gcagtgcctg gaggagcgca acctcaagga 25680

gctggagaag cttctgcagc gcgcgctcaa agacctctgg acgggcttca acgagcgctc 25740

ggtggccgcc gcgctagccg acctcatctt ccccgagcgc ctgctcaaaa ccctccagca 25800

ggggctgcc gacttcacca gccaaagcat gttgcaaaat tttaggaact ttatcctgga 25860

gcgttctggc atcctacccg ccacctgctg cgccctgccc agcgactttg tccccctcgt 25920

gtaccgcgag tgccccccgc cgctgtgggg ccactgctac ctgttccaac tggccaacta 25980

cctgtcctac cacgcggacc tcatggagga ctccagcggc gaggggctca tggagtgcca 26040

ctgccgctgc aacctctgca cgccccaccg ctccctggtc tgcaacaccc aactgctcag 26100

26160

cgcggctccg gggctaaaac tcactccggg gctgtggact tccgcctacc tgcgcaaatt 26220

tgtacctgaa gactaccacg cccacgaaat caggttttac gaggaccaat cccgcccgcc 26280

caaggcggag ctgaccgcct gcgtcatcac ccagggcgag atcctaggcc aattgcaagc 26340

catccaaaaa gcccgccaag agtttttgct gaagaggggt cggggggtgt atctggaccc 26400

ccagtcgggt gaggagctca acccggttcc cccgctgcca ccgccgcgggg accttgcttc 26460

ccaggataag catcgccatg gctcccagaa agaagcagca gcggccgccg ctgccgccgc 26520

cccacatgct ggaggaagag gaggaatact gggacagtca ggcagaggag gtttcggacg 26580

26640

ccgaagccga agaggcaggc gcaacaccgt caccctcggc cgcagccccc tcgcaggcgc 26700

ccccgaagtc cgctcccagc atcagcagca acagcagcgc tataacctcc gctcctccac 26760

cgccgcgacc cacggccgac cgcagaccca accgtagatg ggacaccacc ggaaccgggg 26820

ccggtaagtc ctccgggaga ggcaagcaag cgcagcgcca aggctaccgc tcgtggcgcg 26880

ctcacaagaa cgccatagtc gcttgcttgc aagactgcgg ggggaacatc tccttcgccc 26940

gccgcttcct gctcttccac cacggtgtgg ccttcccccg taacgtcctg cattactacc 27000

gtcatctcta cagcccctac tgcggcggca gtgagccaga ggcggccagc ggcggcggcg 27060

cccgtttcgg tgcctaggaa gacccagggc aagacttcag ccaagaaact cgcggcgacc 27120

gcggcgaacg cggtcgcggg ggccctgcgc ctgacggtga acgaacccct gtcgacccgc 27180

gaactgagga accgaatctt ccccactctc tatgccatct tccagcagag cagagggcag 27240

gatcaggaac tgaaagtaaa aaacaggtct ctgcgctccc tcacccgcag ctgtctgtat 27300

cacaagagcg aagaccagct tcggcgcacg ctggaggacg ctgaggcact cttcagcaaa 27360

tactgcgcgc tcactcttaa ggactagctc cgcgcccttc tcgaatttag gcgggaacgc 27420

ctacgtcatc gcagcgccgc cgtcatgagc aaggacattc ccacgccata catgtggagc 27480

tatcagccgc agatgggact cgcggcgggc gcctcccaag actactccac ccgcatgaac 27540

tggctcagtg ccggcccaca catgatctca caggttaatg acatccgcac ccatcgaaac 27600

caaatattgg tgaagcaggc ggcaattacc accacgcccc gcaataatcc caaccccagg 27660

gagtggcccg cgtccctggt gtatcaggaa attcccggcc ccaccaccgt actacttccg 27720

cgtgattccc aggccgaagt ccaaatgact aactcagggg cacagctcgc gggcggctgt 27780

cgtcacaggg tgcggcctcc tcgccagggt ataactcacc tggagatccg aggcagaggt 27840

attcagctca acgacgagtc ggtgagctcc tcgctcggtc tcagacctga cgggaccttc 27900

cagatagccg gagccggccg atcttccttc acgccccgcc aggcgtacct gactctgcag 27960

28020

gtgccctcgg tctacttcaa ccccttctcg ggctctcccg gtcgctaccc ggaccagttt 28080

atcccgaact ttgacgccgc gagggactcg gtggacggct acgactgaat gtcgggtgga 28140

cccggtgcag agcaacttcg cctgaagcac cttgaccact gccgccgccc tcagtgcttt 28200

gcccgctgtc agaccggtga gttccagtac ttttccctgc ccgactcgca cccggacggc 28260

ccggcgcacg gggtgcgctt tttcatcccg agtcaggtcc gctctaccct aatcagggag 28320

ttcaccgccc gtcccctact ggcggagttg gaaaaggggc cttctatcct aaccattgcc 28380

tgcatttgct ctaaccctgg attacaccaa gatctttgct gtcatttgtg tgctgagtat 28440

aataaaggct gagatcagaa tctactcggg ctcctgtcgc catcctgtca acgccaccgt 28500

ccaagcccgg cccgatcagc ccgaggtgaa cctcacctgt ggtctgcacc ggcgcctgag 28560

gaaataccta gcttggtact acaacagcac tccctttgtg gtttacaaca gctttgacca 28620

ggacggggtc tcactgaggg ataacctctc gaacctgagc tactccatca ggaagaacaa 28680

caccctcgag ctacttcctc cttacctgcc cgggacttac cagtgtgtca ccggcccctg 28740

cacccacacc cacctgttga tcgtaaacga ctctcttccg agaacagacc tcaataactc 28800

ctctccgcag ttccccagaa caggaggtga gctcaggaaa ccccgggtaa agaagggtgg 28860

acaagagtta acacttgtgg ggtttctggt atatgtgacg ctggtggtgg ctcttttgat 28920

taaggctttt ccttccatgt ctgaactatc ccctcttcttt tatgaacaac tcgactagtg 28980

ctaacggggac cctacccaac gaatcgggat tgaatatcgg taaccaggtt gcagtttcac 29040

ttttgattac cttcatagtc ctcttcctgc tagtgctgtc gcttctgtgc ctgcggatcg 29100

ggggctgctg catccacgtt tatatctggt gctggctgtt tagaaggttc ggagaccacc 29160

gcaggtagaa taatgctgct taccctcttt gtcctggcgc tggctgccag ctgccaagcc 29220

ttttccgagg ctgacttcat agagccccag tgcaatatca cttataaatc tgaacgtgcc 29280

atctgtacta ttctaatcaa atgtgttact caacacgata aggtgactgt taaatacaaa 29340

gatcaattaa aaaaagacgc actttacagc agctggcaac caggagatga tcaaaaatac 29400

aatgtaaccg tcttccaggg caaactctcc aaaacttaca attacaattt cccatttgag 29460

cagatgtgtg actttgtcat gtacatggaa aagcagtaca agctgtggcc tccaactccc 29520

cagggctgtg tggaaaatcc aggctctttc tgtatgatct ctctctgtgt aactgtgctg 29580

gcactaatac tcacgcttct gtatctcaga tttaaatcaa ggcaaagctt cattgatgaa 29640

aagaaaatgc cataatcgct caacgcttga ttgctaacac cgggttttta tccgcagaat 29700

gattggaatc accctactaa tcacctccct ccttgcgatt gcccatggggt tggaacgaat 29760

cgaagtccct gtgggggcca atgttaccct ggtggggcct gtcggcaatg ctacattaat 29820

gtgggaaaaa tatactaaaa atcaatggggt ttcttactgc actaacaaaa acagccacaa 29880

gccgagcc atctgcgatg ggcaaaatct aaccttgatt gatgttcaat tgctggatgc 29940

gggctactat tatgggcagc tgggtacaat gattaattac tggagacccc acagagatta 30000

catgcttcac gtagtaaagg gtcccattag cagcccaacc accacctcta ccacacccac 30060

taccaccact actccacca ccagcactgc cgccagcct cctcatagca gaacaaccac 30120

ttttatcaat tccaagtccc actcccccca cattgccggc gggccctccg cctcagactc 30180

cgaccaccacc gagatctgct tctgcaaatg ctctgacgcc attgcccagg atttggaaga 30240

tcacgaggaa gatgagcatg actacgcaga tgcatgccag gcatcagagg cagaagcgct 30300

accggtggcc ctaaaacagt atgcagactc ccacaccacc cccaaccttc ctccaccttc 30360

ccagaagcca agtttcctgg gggaaaatga aactctgcct ctttccatac tagctctgac 30420

atctgttgct attttggccg ctctgctggt gcttctatgc tctatatgct acctgatctg 30480

ctgcagaaag aaaaaatctc acggccatgc tcaccagccc ctcatgcact tcccttaccc 30540

tccagagctg ggcgaccaca aactttaagt ctgcagtagc tatctgccca tcccttgtca 30600

gtcgacagcg atgagcccca ctaatctaac agcctctgga cttacaacat tgtctcttaa 30660

tgagaccacc gctcctcaag acctgtacga tggtgtctcc gcgctggtta accagtggga 30720

tcacctgggc atatggtggc tcctcatagg agcagtgacc ctgtgcctaa tcctggtctg

gatcatctgc tgcatcaaaa gcagaagacc caggcggcgg cccatctaca ggcccttcgt 30840

catcacacct gaagataatg atgatgatga caccacctcc aggctgcaga gcctaaagca 30900

gctactcttc tcttttacag catggtaaat tgaatcatgc cccgcatttt catctacttg 30960

cttctccttc cactttttct gggctcctct acattggcca ctgtgtccca catcgaggta 31020

gactgcctca cgcccttcac agtctacctg cttttcggct ttgtcatctg cacctttgtc 31080

tgcagcgtta tcactgtagt gatctgcttc atacagtgca tcgactacat ctgtgtgcgg 31140

gtggcctact ttagacacca cccccagtat cgcaacaggg acatagcggc tctcctaaga 31200

cttgtttaaa tcatggccaa attacctgtg attggtcttc tgattatctg ctgcgtccta 31260

gccgcgattg ggactcaacc taataccacc accagcgctc ccagaaagag acatgtatcc 31320

tgcagcttca agcgtccctg gaatataccc caatgcttta ctgatgaacc tgaaatctct 31380

ttggcttggt acttcagcgt caccgccctt ctcatcttct gcagtacggt tattgctctt 31440

gccatctacc cttcccttaa cctgggctgg aatgctgtca actctatgga atatcccacc 31500

31560

caaaatcagt ttcgccctcc gtcccctacg cccactgagg tcagctactt taatctaaca 31620

ggcggagatg actgaaaacc tagacctaga aatggacggt ctctgcagcg agcaacgcac 31680

actagagagg cgccggcaaa aagcagagct cgagcgtctt aaacaagagc tccaagacgc 31740

cgtggccata caccagtgca aaaaagggct cttctgtctg gtaaaacagg ccacgctcac 31800

ctatgaaaaa acaggtgaca cccaccgcct aggatacaag ctgcccacac agcgccaaaa 31860

gtttgccctt atgataggtg aacaacccat caccgtcacc cagcactccg tggagacaga 31920

aggctgcatt catgctccct gcaggggcgc tgactgcctc tacaccttga tcaaaaccct 31980

ctgcggtctc agagacctta tccctttcaa ttgatcataa ctgtaatcaa taaaaaatca 32040

cttacttgaa atctgatagc aagactctgt ccaatttttt cagcaacact tccttcccct 32100

cctcccaact ctggtactct aggcgcctcc tagctgcaaa cttcctccac agtctgaagg 32160

32220 gaatgtcaga ttcctcctcc tgtccctccg cacccacgat

aacgcgcgag atcgtctgac gagaccttca accccgtgta cccctacgat accgagatcg 32280

ctccgacttc tgtccctttc cttacccctc cctttgtatc atccgcagga atgcaagaaa 32340

atccagctgg ggtgctgtcc ctgcacctgt cagagcccct taccacccac aatggggccc 32400

tgactctaaa aatggggggc ggcctgaccc tggacaagga agggaatctc acttcccaaa 32460

32520

ccgcacccct cgccgtcagc tccggggccc taaccctttt tgccactccc cccctagcgg 32580

tcagtggcga caaccttact gtgcagtctc aggcccctct tactttggaa gactcaaaac 32640

taactctggc caccaaagga cccctaactg tgtccgaagg caaacttgtc ctagaaacag 32700

agcctcccct gcatgcaagt gacagcagta gcctgggcct tagcgtcacg gccccactta 32760

gcattaacaa tgacagccta ggactagaca tgcaagcgcc catcagctct cgagatggaa 32820

aactggctct aacagtggcg gcccccctaa ctgtggccga gggtatcaat gctttggcag 32880

tagccacagg taatggtatt ggactaaatg aaaccaacac acacctgcag gcaaaactgg 32940

tcgcgcccct aggctttgat accaacggca acattaagct aagcgtcgca ggaggcatga 33000

ggctaaacaa taacacactg atactagatg taaactaccc atttgaggct caaggccaac 33060

tagtagtcat aacctaacca 33120

33180

acattaaact aacaaaaggc cttgtgtatg acggaaatgc catagcagtt aatgttggca 33240

aagggctgga atacagccct actggcacaa cagaaaaacc tatacagact aaaataggtc 33300

taggcatgga gtatgacact gagggagcca tgatgacaaa actaggctct ggactaagct 33360

ttgacaattc aggagccatt gtggtgggaa acaaaaatga tgacaggctt actttgtgga 33420

ccacaccggga cccatcgccc aactgtcaga tttactctga aaaagatgct aaactaacct 33480

tggtactgac taaatgtggc agtcaggttg taggcacagt atctattgcc gctcttaaag 33540

33600

33660

gagactcaac taatggcaca ccatatacaa acgcagtggg ttttatgcct aatctactgg 33720

cctatcctaa aggtcaaact acaactgcaa aaagtaacat tgtcagccag gtctacatga 33780

acggggacga tactaaaccc atgacattta caatcaactt caatggcctt agtgaaacag 33840

gggatacccc tgtcagtaaa tattccatga cattctcatg gaggtggcca aatggaagct 33900

acatagggca caattttgta acaaactcct ttactttctc ctacatcgcc caagaataaa 33960

gaaagcacag agatgcttgt ttttgatttc aaaattgtgt gcttttattt attttcaagc 34020

ttacagtatt tccagtagtc attagaatag agcttaatta aactgcatga gaacccttcc 34080

acatagctta aattatcacc agtgcaaatg gaaaaaaatc aacatacctt tttatccaga 34140

tatcaaagaa ctctagtggt cagttttccc ccaccctccc agctcacaga atacacagtc 34200

ctttcccccc ggctggcttt aaacaacact atctcattgg taacagacat atttttaggt 34260

gtaataatcc acacggtctc ttggcgggcc aaacgctggt ctgtgatgtt aataaactcc 34320

ccaggcagct ctttcaagtt cacgtcgctg tccaactgct gaagcgctcg cggctccgac 34380

tgcgcctcta gcggaggcaa cggcagcacc cgatccttga tctataaagg agtagagtca 34440

taatccccca taagaatagg gcggtgatgc agcaacaagg cgcgcagcaa ctcctgccgc 34500

cgcctctccg tacgacagga atgcaacggg gtggtggtct cctccgcgat aatccgcacc 34560

gctcgcagca tcagcatcct cgtcctccgg gcacagcagc gcatcctgat ctcactgaga 34620

34680

ctgtacccaa agctcatggc gggaaggaca gccccccgt gaccatcgta ccagatcctc 34740

aggtaaatca aatgacgacc tctcataaac acgctggaca tatacatcac ctccttgggc 34800

atgagctgat tcaccacctc tcgataccac aggcatcgct gattaattaa agacccctcg 34860

agcaccatcc tgaaccagga agccagcacc tgaccccccg ccaggcactg cagggacccc 34920

ggtgaatcgc agtggcagtg aagactccag cgctcgtagc cgtgaaccat aggctggtc 34980

attatatcca cattggcaca acacagacac actttcatac actttttcat gattagcagc 35040

tcctctctag tcaagaccat atcccaagga atcacccact cttgaatcaa ggtaaatccc 35100

acacagcagg gcaggcctct cacataactc acgttatgca tagtgagcgt gtcgcaatct 35160

ggaaataccg gatgatcttc catcaccgaa gcccgggtct ccgtctcaaa gggaggtaaa 35220

35280

ccaaagggaa cagcggacgt actcatattt cctccagcag aaccaagtgc gcgcgtggca 35340

gctatccctg cgtcttctgt ctcgccgcct gccccgctcg gtgtagtagt tgtaatacag 35400

ccactccctc agaccgtcaa ggcgctccct ggcgtccgga tctataacaa caccgtcctg 35460

cagcgccgcc ctgatgacat ccaccaccgt agagtatgcc aagcccagcc acgaaatgca 35520

ctcactttga cagcgagaga taggaggagc gggaagagat ggaagaacca tgatagtaaa 35580

agaactttta ttccaatcga tcctctacaa tgtcaaagtg tagatctatc agatggcact 35640

ggtctcctcc gctgagtcga tcaaaaataa cagctaaacc acaaacaaca cgattggtca 35700

aatgctgcac aagggcttgc agcataaaat cgcctcgaaa gtccaccgca agcataacat 35760

caaagccacc gcccctatca tgatctatga taaaaacccc acagctatcc accagaccca 35820

tatagttttc atctctccat cgtgaaaaaa tatttacaag ctcctccttt aaatcacctc 35880

caaccaattc aaaaagttga gccagaccgc cctccacctt cattttcagc atgcgcatca 35940

tgattgcaaa aattcaggct cctcagacac ctgtataaga ttgagaagcg gaacgttaac 36000

atcaatgttt cgctcgcgaa gatcgcgcct cagtgcaagc atgatataat cccacaggtc 36060

ggagcggatc agcgaggaca tctccccgcc aggaaccaac tcaacggagc ctatgctgat 36120

tataatacgc atattcgggg ctatgctaac cagcacggcc cccaaatagg cgtactgcat 36180

36240

agcaagaaca tcataaccat gctcatgcaa atagatgcaa gtaagctcag gaacgaccac 36300

agaaaaatgc acaatttttc tctcaaacat gactgcgagc cctgcaaaaa ataaaaaaga 36360

aacattacac aagagtagcc tgtcttacaa tgggatagac tactctaacc aacataagac 36420

gggccacgac atcgcccgcg tggccataaa aaaaattatc cgtgtgatta aaaagaagca 36480

cagatagctg gccagtcata tccggagtca tcacgtgcga acccgtgtag acccccgggt 36540

36600

gacgaagata caacagaata accccatggg ggggaataac aaagttagta ggtgaataaa 36660

aacgataaac acccgaaact ccctcctgcg taggcaaaat agcgccctcc ccttccaaaa 36720

caacatacag cgcttccaca gcagccatga caaaagactc aaaacactca aaagactcag 36780

tcttaccagg aaaataaaag cactctcaca gcaccagcac taatcagagt gtgaagaggg 36840

aaaaatgacg taaatgtgta aaggtcaaaa 36900

aacgcccaga aaaatacaca gaccaacgcc cgaaacgaaa acccgcgaaa aaatacccag 36960

aagttcctca acaaccgcca cttccgcttt cccacgatac gtcacttcct caaaaaatagc 37020

aaactacatt tcccacatgt acaaaaccaa aacccctccc cttgtcaccg cccacaactt 37080

acataatcac aaacgtcaaa gcctacgtca cccgccccgc ctcgccccgc ccacctcatt 37140

atcatattgg cctcaatcca aaataaggta tattattgat gatg 37184

<---

Claims (28)

1. A collection of nucleic acids used as a cancer vaccine, wherein the nucleic acids encode a collection of peptides containing 209 frameshift peptides (FSP) and modified FSPs (mFSP), wherein the frameshift peptides (FSP) ) and modified FSPs (mFSPs) are shown in SEQ ID NOs: 1-209. 2. A collection of nucleic acids according to claim 1, including four nucleic acids encoding polypeptides with amino acid sequences in accordance with: A) SEQ ID NO: 1088-1091, B) SEQ ID NO: 1092-1095, C) SEQ ID NO: 1155-1158, or D) SEQ ID NO: 1159-1162. 3. The collection of nucleic acids according to claim 1 or 2, where all or part of the collection of nucleic acids is inserted into one or more expression vectors, and the expression vectors in the aggregate contain the entire collection of nucleic acids according to claim 1. 4. The collection of nucleic acids according to claim 3, wherein at least one of the expression vectors contains one or more elements that increase the immunogenicity of the expression vector. 5. The collection of nucleic acids according to claim 3 or 4, where at least one of the expression vectors contains one or more nucleic acids encoding an element that increases the immunogenicity of the peptide collection: an invariant chain sequence or a fragment thereof; tissue plasminogen activator; PEST sequence; boxing destruction of cyclin; ubiquitination signal; sumoylation signal; an interleukin or a specific ligand for a checkpoint protein. 6. The collection of nucleic acids according to claim 5, where the interleukin is interleukin 2, interleukin 12 or interleukin 15. 7. A collection of nucleic acids according to claim 5, wherein the ligand specific for the checkpoint protein is an anti-PD1 antibody or a PD1-binding fragment thereof, an anti-CTLA4 antibody or a CTLA4-binding fragment thereof, an anti-LAG3 antibody or its LAG3- a binding fragment or an anti-TIM3 antibody or a TIM3-binding fragment thereof. 8. Collection of nucleic acids according to any one of paragraphs. 2-7, where each expression vector is selected from the group consisting of a plasmid; cosmids; RNA; RNA formulated with an adjuvant; RNA compiled into liposomal particles; self-amplifying RNA (SAM); SAM formulated with an adjuvant; SAM formulated into liposomal particles; and viral vector. 9. The collection of nucleic acids according to claim 8, where the viral vector is an alphavirus vector, a Venezuelan equine encephalitis virus (VEE) vector, a Sindbis virus (SIN) vector, a Semliki Forest Virus (SFV) vector, replication-competent or replication-incompetent adenovirus vector, poxvirus vector, vaccinia vector or modified vaccinia Ankara (MVA) vector, simian or human cytomegalovirus (CMV) vector, lymphocytic choriomeningitis virus vector ( LCMV) or a retrovirus or lentivirus vector. 10. A collection of nucleic acids according to claim 9, wherein the replication-competent or replication-incompetent adenovirus vector is derived from a chimpanzee or a bonobo or a gorilla. 11. The use of a collection of nucleic acids according to any one of paragraphs. 1-10 for the prevention or treatment of a patient with cancer involving cancer cells with an MSI phenotype, or at risk of developing such cancer. 12. The use of a collection of nucleic acids according to any one of paragraphs. 1-10 for the prevention or treatment of a patient with or at risk of developing cancer with an MSI phenotype, wherein the collection of nucleic acids is intended to be administered using a heterologous prime-boost vaccination schedule. 13. Use according to claim 12, wherein the priming is performed with an adenovirus vector and one or more boosters are performed with a poxvirus vector. 14. Use according to claim 13, wherein the poxvirus vector is an MVA vector. 15. Application according to any one of paragraphs. 11-14, where prophylaxis is for patients known to be at risk of developing cancers with the MSI phenotype, in accordance with clinical guidelines, including patients with gametic mutations in genes involved in the mismatch repair (MMR) system, such as MLH-1, MSH-2, MSH-6, PMS2 and TACSTD1/EPCAM. 16. Application according to any one of paragraphs. 11-14 where treatment is for patients with all stages of cancer (I-IV) occurring in any tissue after diagnosis of MSI status in accordance with the most recent clinical guidelines. 17. Use according to claim 16, wherein the use of such a vaccine is for the treatment of forms of cancer with MSI status, which may be either spontaneous or pharmacologically induced. 18. Application according to any one of paragraphs. 11-17, wherein the cancer is selected from the group consisting of colon and rectal cancer, gastric cancer, endometrial cancer, small intestine cancer, hepatobiliary tract cancer, liver cancer, neuroendocrine cancers, cervical cancer, ovarian cancer, forms of uterine sarcoma, brain cancer and skin cancer. 19. A collection of peptides for use as a cancer vaccine containing 209 frameshift peptides (FSP) and modified FSPs (mFSP), wherein the frameshifted peptides (FSP) and modified FSPs (mFSP) are shown in SEQ ID NO: 1-209. 20. A collection of peptides according to claim 19, including four polypeptides with amino acid sequences in accordance with: A) SEQ ID NO: 1088-1091, B) SEQ ID NO: 1092-1095, C) SEQ ID NO: 1155-1158, or D) SEQ ID NO: 1159-1162.

Images