RU2790976C1 - Method for keeping service dogs - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention relates to the field of veterinary medicine, in particular it concerns the treatment and health prevention of service dogs. A method for keeping service dogs includes introduction into diet of stimulating additives. At the same time, in addition to the main diet, dogs are given “Ecolit-vet” preparation with water, in courses for 10 days straight. Preventive drinking of the preparation is performed once a day in amount of 0.25 ml per kg of live weight of a dog, using “Ecolit-vet” neutral anolyte diluted with water in a ratio of 1:10. The used “Ecolit-vet” preparation has pH acidity of 7.2-8.4 and active chlore content of 100 mg/l.

EFFECT: invention provides optimization of metabolic processes in dogs, increase in the immunity and resistance, as well as reduction in inflammatory and allergic reactions.

1 cl, 4 tbl

Description

The invention relates to the field of veterinary medicine, in particular, concerns the maintenance, prevention and treatment of service dogs.

Currently, the problem of maintaining the health of service animals remains relevant, because. various diseases lead not only to a decrease in the productivity of animals, but also to a decrease in the quality of their life and service qualities, leads to impaired digestion, assimilation of food eaten, and in the absence of medical assistance can lead to exhaustion, and ultimately to the death of animals, therefore, to This problem has attracted the attention of veterinary specialists around the world.

On the domestic market there is a significant amount of vitamin-containing preparations and dietary supplements, the use of which has a general strengthening and stimulating effect on the body of service dogs, increases resistance and prevents the occurrence of diseases and stressful situations.

Known in particular are Gamovit and Katozal, which are widely used and known to cynologists. (See Nosyreva A.A. Zhuravleva O.N. Extending the term of service dogs use // International Student Scientific Bulletin - 2017. - No. 2).

The use of dietary supplements (biologically active additives) Omega Neo and Radostin in the form of tablets for 30 days in the feeding of service dogs is also known. (See Safargalina E.M. The use of biologically active additives in the diet of young service dogs // Student Scientific Forum - 2017).

The “Method for correcting metabolic processes in dogs” is also known (see Patent of the Russian Federation No. 2739028; Published on December 21, 2020. Bull. No. 35.), in which, in addition to the diet, the animals are injected with a phytomineral feed additive from the BSh-VIT component and sea buckthorn berry powder in for 21 days once a day.

With a fairly wide use of such drugs, the disadvantages are their rather high cost and labor costs for additional maintenance and maintenance of service dogs.

The objective of the invention is to expand the range of domestic drugs for the prevention of diseases and after stressful situations in service dogs without the use of antibiotics, and hepatotactic sulfanilamide drugs with minimal costs for prevention.

The problem is solved by the fact that as a therapeutic and prophylactic agent for service dogs, the drug "Ecolit-vet" is used in dilution of 1 part of the drug and 10 parts of drinking water, drinking the drug to dogs weakened after stress and increased load at a dose of 0.25 ml / kg of live masses with water, regardless of the time of feeding, 1 time per day for 10 days using "Ecolit-vet" obtained using mobile electrochemical reactors (hereinafter referred to as installations) of the STEL type, manufactured by Water Technology LLC, Yekaterinburg, with acidity ( pH 7.2 - 8.4) and an active chlorine content of 100 mg/l.

"Ekolit-vet" - electrochemically activated water (or a weak saline solution) has not only oxidizing, reducing, acidic and alkaline properties, but also has pronounced catalyst properties in both chemical and biochemical reactions, and the resulting anolyte has high antimicrobial activity against bacteria, viruses, spores, fungi.

The use of electrochemically activated sodium chloride solutions as therapeutic and prophylactic agents is permitted by the Veterinary Council of the Department of Veterinary Medicine of the Ministry of Agriculture and Food of the Russian Federation (Minutes No. 2 dated April 23, 1999, registration No. PVR-2-2.9 / 00088.

The properties of electroactivated solutions determine an extremely wide therapeutic range of their application: from surgery, obstetrics and gynecology, internal non-communicable diseases - to infectious and parasitic diseases and as a preventive measure.

In the past decades, the main methods for achieving the highest productivity of animals have been the use of biological growth stimulants, feed antibiotics, hormones, the introduction into the diet of only those feeds that contributed to the highest yield of the required products, without taking into account their effect on the population of intestinal bacteria. However, after prolonged use of these biologically active drugs, it turned out that they lead to an increase in stress loads on the body and a stable population of intestinal diseases. The widespread use of antibiotics in animal husbandry and veterinary medicine has led to the transfer of antibiotic resistance from strains of microorganisms of animal origin to microbial strains of the human population.

"Ekolit-vet" is a 20-22% aqueous solution of sodium chloride, subjected to electrochemical action in the cathode and anode chambers of the diaphragm reactor, as a result, the drug is enriched with oxidants (HClO, Cl ₂ O, ClO ₂ , Cl, O ₂ , O ₃ , OH ). Guaranteed indicators: the concentration of hydrogen ions - pH from 7.2 to 8.4, the concentration of active chlorine 0.01%.

Used in practical conditions, "Ekolit-vet" are characterized by indicators of the concentration of hydrogen ions (pH), the value of the redox potential (ORP) in mV and the concentration of active chlorine in (in mg / l).

To obtain "Ecolit-vet", artesian water is used according to GOST 2874-82 "Drinking water" and food table salt according to GOST 13830-91.

These settings allow you to get a neutral (pH 7.2-8.4 units) "Ecolit-vet". Subject to the passport regime and technological requirements in operation, the installations make it possible to obtain "Ekolit-vet" with an active chlorine content of 100 mg/l.

A high effect from the use of this water has been achieved in the treatment of wounds, ulcers, bedsores, a number of cardiovascular, gynecological, allergic, respiratory, gastrointestinal diseases, arthritis, radiculitis, stomatitis, prostate adenoma, tonsillitis, influenza, infectious hepatitis and other diseases. . In veterinary practice, many aspects of this problem are poorly understood. The use of electroactivated water for the treatment of sick animals is very limited. There are practically no fundamental works on the study of the effect of electroactivated water on pathogens of infectious animal diseases and its use in veterinary medicine. There are only a few reports on the use of activated acidic and alkaline water in veterinary surgical practice, as well as for the treatment of animals with gastrointestinal diseases.

Apply "Ekolit-vet" (electrochemical activated) solution of anolyte (neutral), pH 7.2-8.4), synthesized on STEL devices, which has antimicrobial (bactericidal, virucidal, sporicidal) and detergent properties. Anolyte (neutral) is used for disinfection in accordance with guidelines. The essence of "Ekolit-vet" is that the liquid flowing through the diaphragm electrolyzer, when exposed to a high voltage electric field, goes into a metastable (activated) state with abnormally high oxidizing (for anolyte) and reducing (for catholyte) properties. In this case, the electrical energy of a non-equilibrium electrochemical action can be accumulated and stored in the liquid in the form of internal potential energy, which is realized in various catalytic reactions during the period of liquid relaxation (transition to an inactivated state.

The data of literary sources indicate that in various acute and chronic diseases of the gastrointestinal tract of animals, electrochemical activation of aqueous salt solutions is the most successful rational technology that allows you to reduce both material (economic costs), energy, and labor to obtain highly effective new drugs in veterinary medicine.

The study of literary sources revealed the need to develop scientific foundations and methodological principles for the use of electroactivated water in veterinary medicine.

Thus, as shown by the literature review, there is no consensus among scientists about the effect of structured water on metabolic processes in carnivores (dogs). As a result of numerous studies conducted in the laboratories of the Ural Federal Research Center of the Ural Branch of the Russian Academy of Sciences with testing in production and laboratory conditions of a new environmentally friendly product "Ekolit-vet" (neutral anolyte), in order to study the optimization and normalization of metabolic processes, increase their efficiency, doses of application and multiplicity of the preparation "Ekolit-vet" (neutral anolyte). The method of its application has been worked out, positive results have been obtained.

Currently, an important problem is the search, testing and use of inexpensive, at the same time effective feed additives. The unipolar electrochemical activation (ECA) of Ecolit-vet liquid is based on known electrolysis reactions. The electrolysis of concentrated solutions ends with obtaining a pure finished product, and weak (up to 5 g / l) aqueous solutions of salts or ordinary drinking water, which contains up to 1 g / l of various salts in its composition, is subjected to electrochemical activation. The purpose of the ECA is to bring the liquid into an activated state.

It has been established that ECA allows, without additional costs of chemical reagents, to convert fresh or slightly saline water into a highly active technological solution with many functional properties, which makes it possible to use the drug as a feed additive.

To conduct tests on service dogs, we used Ecolit-vet provided by Water Technologies LLC (Yekaterinburg).

"Ekolit-vet" is a 20-22% aqueous solution of sodium chloride, subjected to electrochemical action in the cathode and anode chambers of the diaphragm reactor; subjected to electrochemical action in the cathode and anode chambers of the diaphragm reactor; as a result (ecolite) is enriched with oxidants (HClO, Cl ₂ O, ClO ₂ , Cl, O ₂ , O ₃ , OH). Guaranteed indicators: the concentration of hydrogen ions - pH from 7.2 to 8.4, the concentration of active chlorine 0.01%.

Used in practical conditions, "Ekolit-vet" are characterized by indicators of the concentration of hydrogen ions (pH), the value of the redox potential (ORP) in mV and the concentration of active chlorine in (in mg / l).

To obtain "Ecolit-vet", artesian water is used according to GOST 2874-82 "Drinking water" and food table salt according to GOST 13830-91.

"Ekolit-vet" is produced using mobile electrochemical reactors (hereinafter referred to as installations) of the STEL type with a capacity of 20, 40, 60, 80, 250 and more l / h (STEL-10 AK, STEL-10N, etc.), which are commercially produced by Water Technologies LLC (Yekaterinburg).

These settings allow you to get a neutral (pH 7.2-8.4 units) "Ecolit-vet". Subject to the passport regime-technological requirements of operation, these plants make it possible to obtain "Ekolit-vet" with an active chlorine content of 100 mg/l.

In the experiments we used:

- service dogs 2-3 years old (23-42 kg).

The work was carried out at the enterprises of the Sverdlovsk region and in the laboratories of immunology and pathobiochemistry of the FBGNU UrFARC UB RAS.

The objects of research were 10 service dogs aged 2-3 years, selected on the principle of pairs of analogues.

From the marked livestock, 2 groups of animals (control and experimental) were formed in each, service dogs of 5 heads. The conditions for feeding, keeping and caring for the animals of the experimental and control groups were identical.

Applied to the additive "Ekolit-vet" in a mixture with drinking water. The duration of the prophylactic course is 10 days.

"Ekolit-vet" was fed with water individually using existing technologies, based on:

- for dogs of medium and large breeds 2-3 years of age (23-42 kg) - daily dose of 0.25 ml / kg of live weight, diluted 1:10.

The studies were aimed at studying the effect of the "Ecolit-vet" additive on the morphological, biochemical parameters of the body of service dogs and the normalization of metabolic processes. The scheme of the experiment is shown in Table 1.

Blood for the study was taken from the animals of the control and experimental groups before the start of the experimental work, and after 15-35 days.

When performing experimental work, the following research methods were used:

Hematological studies were performed on the analyzer "Abacus Junior Vet" company "Diatron" (Austria). The leukocyte formula of the percentage of cells was calculated in blood smears stained according to Romanovsky-Giemsa, the count was carried out on a microscope MS 50 (MICROS, Austria). The immunological parameters of the blood were determined in accordance with the Guidelines "Panel of the most informative tests for assessing the resistance of animals" (Novosibirsk, 2007). The reactions of the cellular link of immunity were taken into account on the microscope MS 50 (MICROS, Austria), the centrifuge "Dastan" laboratory clinical OPn-3.04.

Biochemical studies of blood in animals were carried out using kinetic, colorimetric and turbidimetric research methods. Automatic biochemical analyzer ChemWellCombi manufactured by Awaveness Technology (USA). Reagent kits from VitalDiagnosticsSpb, Diasys (Germany).

Mathematical and statistical processing of the obtained data was carried out using the Microsoft Excel 2010 application program.

A production test on service dogs was carried out in the canine unit of FKU IK-10 GUFSIN (Yekaterinburg) from 08/31/2020 to 10/05/2020. The content of service dogs in FKU IK-10 of the GUFSIN complies with the requirements of the order of the Federal Penitentiary Service of Russia dated December 31, 2019 No. 1210 “On approval of the procedure for handling service animals in institutions and bodies of the penitentiary system of the Russian Federation”, feeding is organized in accordance with the standards approved by order of the Federal Penitentiary Service of Russia dated 05/13/2008 No. 330 "On approval of the norms for the provision of feed (products) and the norms for the replacement of feed (products) when providing regular animals of institutions and bodies of the penitentiary system in peacetime."

2 groups of service dogs were formed, 23 - 42.0 kg (control and experimental), 5 animals each. The conditions for feeding, keeping and caring for the animals of the experimental and control groups were identical. Animals of the experimental group additionally fed the prepared solution at a dose of 0.25 ml/kg of body weight per day, at a dilution of 1:10, for 10 days.

During the experiment, the animals were kept on diets balanced in terms of essential nutrients, macro- and microelements, vitamins A, D, E.

Blood for the study was taken from the animals of the control and experimental groups before the start of the experimental work and after 35 days. The effect of "Ecolit-vet" on hematological blood parameters of service dogs is presented in Table 2.

Evaluation of the effectiveness of the use of "Ecolit-vet" was carried out according to the morphological, biochemical parameters of the body of dogs, in order to optimize metabolic processes, increase safety, increase the growth of animals.

The number of leukocytes (Table 2) in the experimental group increased by 27.1%, the number of segmented neutrophils by 40.7%, which is a sign of increased immunity, in the control group there was a decrease by 15.4% and 8.7%, respectively. The number of lymphocytes in the experimental group decreased by 31.8%, ESR decreased by 25.7%, which indicates a decrease in inflammatory reactions in the body of dogs, in the control group the number of lymphocytes increased by 18.2%, ESR by 14.7%. In the experimental group, the number of eosinophils decreased by 20.2%, which indicates a decrease in allergic reactions in the body of animals, in the control group it increased by 41%.

Immunological parameters of the blood of dogs in the application of "Ecolyte-vet" are shown in Table 3.

The number of circulating immune complexes in the blood of working dogs of the experimental group decreased by 13.7%, which indicates a decrease in inflammatory reactions in the body of dogs, in the control group it increased by 17.6%. The ratio of T-lymphocytes to B-lymphocytes in the experimental group increased by 11.5%, which indicates an increase in humoral immunity, in the control group by 6.2%. Phagocytic activity in dogs of the experimental group increased by 34.7%, in the control group it decreased by 12.4%.

Biochemical parameters of the blood of dogs in the application of "Ecolit-vet" are shown in Table 4.

In the experimental group, the content of creatinine in the blood serum of dogs was above the physiological norm, after the experiment it decreased by 34.4%, urea by 20.4%, which is a sign of normalization of kidney function, in the control group it decreased by 19.2% and 15. 9% respectively. The content of iron in the blood serum of the experimental and control groups before the experiment was above the physiological norm, according to the results of the experiment in the experimental group, the concentration of iron decreased by 9.4%, which indicates the normalization of mineral metabolism, while in the control group it increased by 7% . In the experimental group, ALT decreased by 8.5%, which is a consequence of the normalization of liver function, in the control group it increased by 2.6 times. Before the experiment in both groups, the content of alkaline phosphatase was above the norm, after the experiment in the experimental group its content decreased by 48.2% to the level of physiological variability, which indicates the normalization of liver functions, in the control group the concentration of alkaline phosphatase increased by 22.4%.

Complications or side effects in the application of "Ecolit-vet" have not been established.

Safety among service dogs was 100% in both groups.

Based on the studies conducted to study the effectiveness of the use of Ecolit-vet, it should be concluded that Ecolit-vet, when fed to service dogs at a dose of 0.25 ml / kg of live weight per day at a dilution of 1:10, provides optimization of metabolic processes, increased immunity and resistance, reducing inflammatory and allergic reactions.

Drinking "Ekolit-vet" to animals stimulated the processes of erythropoiesis, leukopoiesis and activated protein metabolism in the body, there was an activation of nonspecific resistance of the animal organism.

Immunohematological studies on the "Ecolit-vet" test on dogs indicate a decrease in possible inflammatory processes, and an increase in nonspecific resistance of the body associated with infectious pathology in animals.

The study of the dynamics of blood biochemical parameters in experimental animals after the use of "Ecolit-vet" revealed a positive effect of the drug on the amount of total protein, glucose, AST, Alt, GTTP, calcium, phosphorus, albumin and globulins.

The inclusion of Ecolit-vet in the diet is safe and does not cause negative effects on the animal organism. The study drug used according to the proposed method had a stimulating effect on the biochemical processes in the body of animals and birds, which resulted in the optimization and normalization of metabolic processes. The use of the additive "Ekolit-vet" additionally improved the metabolic processes in the animal body.

The novelty of the method is the use of Ecolit-vet for a new purpose as a stimulating additive for service dogs with an unobvious effect in the form of optimizing metabolic processes, increasing immunity and resistance of the dog's body, and as a result, the proposed method for keeping service dogs can be recommended for widespread use in cynological services of GUIN and other units when keeping service and working dogs.

Claims (2)

1. A method of keeping service dogs, including the introduction of stimulating additives into the diet, characterized in that, in addition to the main diet, the dogs are fed the Ecolit-vet remedy with water in courses for 10 days in a row, and preventive drinking of the drug is carried out once a day at the rate of 0.25 ml per kg of live weight of the dog, using the neutral anolyte "Ekolit-vet", diluted with water at the rate of 1:10. 2. The method of keeping service dogs according to claim 1, characterized in that the drug "Ecolit-vet" is used with an acidity of pH 7.2-8.4 and an active chlorine content of 100 mg/l.