RU2772743C1 - Portable electric heating device for loop-mediated isothermal amplification and application of this device - Google Patents

Abstract

FIELD: biochemistry.

SUBSTANCE: invention relates to the field of biochemistry, namely to a portable device for conducting loop-mediated isothermal amplification and its application. The portable device contains a body of two parts, the upper of which is tightly seated in the lower. The device contains an insert in the form of two cone-shaped rubber cylinders for attaching test tubes, a working chamber with a temperature sensor, an upper cover, an electronic board, a lower cover, a connector for power supply and tubular heating elements. The board contains a thermostat unit, a converter unit, a controller unit, a power connector unit, two LED units, blue and red.

EFFECT: expansion of the range of portable heating devices.

2 cl, 3 dwg

Description

The invention relates to the field of biochemistry, namely to portable devices for loop isothermal amplification and can be used both in the laboratory and at home.

Prior Art

Known RF patent for invention No. 2666925, IPC C12M 1/38, C12Q 1/68, B01L 7/00. "DEVICE FOR CARRYING OUT A POLYMERASE CHAIN REACTION WITH A ONE-DIRECTIONAL SLIDING MEANS".

A device for carrying out a polymerase chain reaction is proposed. The PCR device contains series-connected PCR thermoblocks, containing two or more reaction chambers, a plate-type PCR chip and a unidirectional sliding means. Each PCR thermoblock contains two or more heaters located on the upper surface of the substrate. Sliding means provides serial thermal contact between two or more reaction chambers repeatedly located from one to the other edge of the PCR chip with two or more heaters repeatedly located from one to the other edge of the PCR thermoblock. The invention provides a reduction in the duration of PCR, an increase in the yield of PCR and an increase in the number of samples measured and analyzed in real time.

The disadvantage of this device is the complexity in manufacturing and use.

Closest to the claimed invention is the RF patent for the invention No. 2305233, IPC F25D 3/14, G05D 23/30. "PORTABLE THERMOSTAT FOR BIOLOGICAL RESEARCH".

The technical result is to simplify the device, increase reliability and reduce the installed power while reducing material consumption and cost. A portable thermostat for biological research consists of a housing with thermal insulation, inside of which there is a working chamber, access to which is through a thermally insulated door, technological shelves are located inside the working chamber, slightly shorter than the inner space of the working chamber, which form air channels, air which flows around the temperature sensor from the working chamber due to an electric fan and is heated by an electric heater, a switching unit connected to the mains power supply buses, a DC amplifier, a power filter, a control unit, a temperature adjuster, a rectifier, and a step-down transformer are also introduced.

The disadvantages of the proposed thermostat are its size, manufacturing complexity, namely, a large number of sensors, regulators, etc. and the complexity of using the thermostat, which consists in performing a certain number of actions, the sequence of which can be confused with individual use.

Disclosure of invention

The technical objective of this invention is to create

· easy-to-use portable electric heating device for loop isothermal amplification.

· easy-to-manufacture portable electric heating device for loop isothermal amplification.

· reliable and non-energy portable electric heating device for loop isothermal amplification.

The stated technical problem is solved due to the fact that a portable electric heating device for carrying out loop isothermal amplification contains a device body consisting of two parts, the upper of which is tightly seated in the lower one, thereby forming a sealed reservoir. The upper part of the housing contains an insert in the form of two cone-shaped rubber cylinders for attaching test tubes, and the top cover is equipped with two seals. The working chamber with a temperature sensor is located in the upper part of the housing. The lower part of the case contains the electronic board, the bottom cover, the power connector and the coils that open into the upper part of the case. The board contains a thermostat block, a converter block, a controller block, a power connector block, two blocks of blue and red LEDs.

The use of a portable electric heating device for carrying out the loop isothermal amplification reaction consists in pouring water into the upper part of the body and connecting the device to the network. The water begins to heat up and the red LED on the housing flashes. The temperature sensor constantly transmits information about the current water temperature to the controller unit, and then to the electronic board thermostat unit. And the thermostat unit transmits the desired voltage to the heaters, until the water in the tank is heated to 65 degrees C. After the required temperature is reached, the red LED starts to burn statically. Then the test tubes are inserted into the rubber cylinders and the reaction takes place for 25 minutes, with the red LED lit statically. When the reaction time has elapsed, the red LED goes out and the blue one lights up. The device is disconnected from the mains, and the test tubes are removed from the device and the water is drained from the reservoir.

Figure 1 shows the elements of a portable electric heater for loop isothermal amplification.

Figure 2 is a schematic representation of a portable electric heating device for loop isothermal amplification.

In FIG. 3 is a functional diagram of a portable electric heating device for loop isothermal amplification.

Implementation of the invention

Portable electric heating device 1 for carrying out loop isothermal amplification, contains a body 2 of the device 1, consisting of two parts, the top 5 of which is tightly seated in the bottom 6, thereby forming a sealed reservoir 8. cone-shaped rubber cylinders for attaching test tubes, and the top cover 3 is equipped with two seals 10. The working chamber 4 with a temperature sensor 11 is located in the upper part of the housing 2. The lower part of the housing 6 contains the electronic board 12, the bottom cover 7, the power supply connector 13 and the heater 1 14 and ten 2 15, which go into the upper part of the case 2. Board 12 contains a thermostat block 17, a converter block 18, a controller block 19, a power connector block 20 and two blocks of LEDs blue 22 and red 21.

The use of a portable electric heating device 1 for carrying out the loop isothermal amplification reaction consists in the fact that water is poured into the upper part of the housing 5 and the device 1 is connected to the network. The water starts to heat up and the red LED 21 on the housing 1 flashes. The temperature sensor 11 constantly transmits information about the current water temperature to the controller unit 19, and then to the thermostat unit 17 of the electronic board 12. And the thermostat unit 17 transmits the desired voltage to heaters 1 and 2 (14 and 15), until the water in the tank 8 is heated up to 65 degrees C. After reaching the required temperature, the red LED 21 starts to burn statically. Then the test tubes are inserted into the rubber cylinders 9 and the reaction takes place for 25 minutes, with the red LED 21 lit statically. After the reaction time has elapsed, the red LED 21 goes out and blue 22 lights up. The device 1 is disconnected from the mains, and the test tubes are removed from the device 1 and the water from the reservoir 8 is drained.

The device is used as follows.

The device 1 is preliminarily connected to the power supply, while it is possible to connect to the network, to a PC, telephone and other devices containing a rechargeable battery.

The red LED 21 will start flashing (at this time the device is reaching the required temperature). When the red LED 21 stops flashing and lights up statically, device 1 is ready for operation.

A test tube with a reagent is placed in a sealed tank 8 with water heated to the desired temperature (65°C) through an insert in the form of two rubber cylinders 9 on the upper part of the body 5 so that the test tubes are completely placed in it.

At the end of the work, the blue LED 22 starts to burn - this is an indicator of the end of the work. After that, it is necessary to turn off the device 1 from the mains, remove the test tubes and drain the water.

The electric heater is a disposable device.

The advantages of the proposed solution are:

1. Carrying out loop isothermal amplification both at home and in the laboratory.

2. The ability to use the device without connecting to a 220 V network.

3. Compactness of the device.

4. Easy to manufacture and transport.

5. The device is easy to use, and has access to all replaceable or adjustable elements.

6. The design of the device ensures the interchangeability of interchangeable products of the same name.

7. The design of the device provides resistance to thermal and electrodynamic loads arising from short-circuit currents.

8. The design of the device provides convenience and safety of operation and maintenance, ease of monitoring the operation of the product, ease of installation of the product, as well as connection of external connections, access to contact connections, ease of repair and replacement of components.

9. The design of the device ensures safety due to the fact that the wires and cables are protected from kinks and exposure, breaking the insulation.

10. Ability to scale research by connecting multiple instruments.

All the above descriptions of the functioning of the device confirm the fulfillment of the technical task of the present invention, namely, the creation of an easy to use and manufacture, as well as a reliable and low-energy portable electric heating device for loop isothermal amplification.

Industrial Applicability

All of the above indicates the industrial applicability of the device for loop isothermal amplification.

List of positions

1 - device for loop isothermal amplification

2 - body

3 - top cover

4 - working chamber

5 - upper part of the body

6 - lower part of the body

7 - bottom cover

8 - sealed tank

9 - insert in the form of two rubber cylinders

10 - top cover seal

11 - temperature sensor

12 - electronic board

13 - connector for power supply

14 - ten 1

15 - ten 2

16 - secure wired connection

17 - thermostat unit

18 - converter block

19 - controller block

20 - power connector block

21 - red LED block

22 - blue LED block.

Claims (2)

1. A portable electric heating device for carrying out loop isothermal amplification, containing a body with a lid and a working chamber, which contains a temperature sensor, heating elements, a connector with busbars for power supply, characterized in that the body of the device consists of two parts, the upper of which is tightly seated in the bottom, thereby forming a sealed reservoir, while the upper part of the body contains an insert in the form of two cone-shaped rubber cylinders for attaching test tubes, and the top cover is equipped with two seals, the outer diameter of which is equal to the inner diameter of the rubber cylinders, while the working chamber with a temperature sensor is located in the upper part of the case, and the lower part of the case contains an electronic board, a bottom cover, a connector for power supply and shadows that go into the upper part of the case, moreover, the temperature and shade sensor have a secure wired connection with the electronic board, and the board itself contains a thermostatic control unit generator block, converter block, controller block, power connector block and two blocks of blue and red LEDs, while the connections of all working elements of the device are distributed as follows: the heaters have one-way outgoing connection with the temperature sensor and one-way incoming connection with the temperature controller unit, and the temperature sensor is connected with the transducer block, the transducer block, in turn, is connected to the controller block, and the controller block is connected to the thermostat block, the power connector block, and outgoing communication with the LED blocks. 2. The use of a portable electric heating device according to claim 1 for loop isothermal amplification.

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