RU2771010C1 - Drug for treatment of cats with dermatitis of various etiologies - Google Patents

Abstract

FIELD: veterinary medicine.SUBSTANCE: invention relates to the field of veterinary medicine, in particular to dermatology; it is intended for the treatment of cats with allergic dermatitis and viral dermatoses. A drug includes colloid silver, zinc oxide, glycerin, methylcellulose, amino acid lanthanum salt and distilled water in a certain quantitative ratio.EFFECT: implementation of the invention allows for reduction in time and increase in the efficiency of the treatment of allergic dermatitis and viral dermatoses in cats.1 cl, 6 dwg, 4 ex

Description

The field of technology to which the invention belongs

This invention belongs to the field of veterinary medicine, in particular to dermatology, and is an agent for the treatment of cats with dermatitis of various etiologies. The invention can be used as a reparative drug in veterinary clinics for the treatment of cats with dermatitis accompanied by skin lesions.

State of the art

The skin is a complex organ, which is the outer covering of the body, and therefore primarily performs protective functions: protection from mechanical and bacterial influences; the skin is involved in immune protection, thermoregulation, water-salt, fat, vitamin metabolism. The most common diseases that damage the health of pets include skin diseases of various etiologies (Tolkachev V.A., Kolomiytsev S.M., Everstova E.A., Kucheruk D.L. Nosological profile of skin diseases in dogs // Bulletin of the Kursk State Agricultural Academy 2017. P. 25-29.).

Dermatological patients with inflammatory skin diseases are often a "headache" for veterinarians due to the wide variety of symptoms, the possible complications of fungal and bacterial infections, and the difficult diagnostic process. An urgent task is to search for new drugs that can control unwanted symptoms and complications of the disease by influencing the leading links of the pathological process (Lutsko I.A. New drug - new opportunities // VetPharma No. 3 | May–June 2015. P. 36-38).

Known substance for the treatment of dermatitis in animals, which includes the use of a drug containing wt. %: azelastine hydrochloride - 0.05, aqueous extract of eucalyptus ballus leaves - 2.0, benzocaine - 5.0, propolis - 5.0-6.0, vaseline - 87.95-86.95. The drug is used by applying to the affected areas of the skin 2 times a day (see US Pat. RU 2601895, IPC A61K 31/55, A61K 36/61, A61K 31/245, A61K 35/644, A61K 35/06, A61P 17/00 published 01/10/1999).

The disadvantages of this substance according to the method include a long course of treatment and lack of effectiveness in dermatitis in cats of various etiologies with skin lesions.

Known substance for the treatment of skin inflammatory and allergic etiology in cats and dogs "Stop-Itch-2". An agent for the treatment of skin of inflammatory and allergic etiology in cats and dogs contains polcortolone or triamcinolone as a glucocorticosteroid, chloramphenicol and metronidazole as an antibiotic, lidocaine hydrochloride, dimethylformamide, dimethyl sulfoxide, isopropyl alcohol and polyethylene glycol 400 as a filler, additionally contains calendula extract and metronidazole ( see Pat. /00 published 06/27/2010).

The disadvantage of this substance may be local atrophy of the skin with prolonged use of the drug, it is also not recommended for use in animals suffering from diabetes and viral infections.

Known substance according to the method of treatment of wound surfaces, which consists in the treatment of wound surfaces in the stage of inflammation with liniment "Eplan", containing wt. %: lanthanum nitrate hexahydrate in terms of lanthanum 2.2-2.8, triethylene glycol 60-64, ethyl carbitol 23-27, glycerin 1.8-2.2, caustic soda 0.7-0.9, water the rest, and at the stage of reparation, the use of ointment "Eplan-M" composition, wt. %: liniment "Eplan" - 80-85, polyethylene glycol (PEG-115) - 10-20, which leads to a decrease in microbial contamination of wound surfaces, suppression of the manifestations of the inflammatory reaction, a decrease in the duration of the inflammatory process and a reduction in the time for complete healing of wounds (see Pat. RU 2124357, IPC A61K 33/00, A61K 33/10, A61K 31/075, A61L 15/44, A61L 15/20 publ. 01/10/1999).

The disadvantages of this substance according to the method of treating dermatitis include a long course of treatment, the need to use two dosage forms of the drug.

Known substance according to the method of treatment of rapidly progressive periodontitis (PPD). The invention relates to medicine, namely to dentistry, and is intended for the treatment of rapidly progressive periodontitis (RPD). For the treatment of PPP, periodontal pockets are treated using an ultrasound system. Then they are washed with an antiseptic solution heated to a temperature ^of 38-40 ° C. The mixture is laid in the form of a slurry prepared extempore containing 250 mg of 2-methyl-5-nitro-1H-imidazole-1-ethanol (1 tablet of the drug "Metronidazole") , mixed with 5-7 drops of a liquid form of a complex compound of lanthanum nitrate and triethylene glycol in glycerin (Eplan preparation), 4-5 times a week. Ossein-hydroxyapatite compound (Osteotenon preparation) is prescribed at 830-1660 mg (1-2 tablets) for 1-2 months. The use of the invention makes it possible to increase the effectiveness of treatment due to the impact on all links of etiopathogenesis, to ensure a quick stop in the development of the disease and the possibility of preventing further development of the disease (see US Pat. /02, published on August 22, 2017).

The disadvantages of the substance according to the method of treating dermatitis include a long course of treatment, the need to use three drugs and, in fact, the impossibility of using it for the treatment of dermatitis in cats.

A substance is known according to the method for disinfecting the skin from non-spore-forming and spore-forming microorganisms, for the prevention of infectious diseases caused by spore-forming bacteria, and for the healing of wound defects of the skin. Pharmaceutical composition containing Eplan as a chemotherapeutic agent and a safe and effective amount of a quaternary ammonium compound of the general formula [RR1N+R2R3]X- as a surfactant, where R, R1, R2, R3 are organic substituents; X - OH hydroxide, or halogen, or alkyl phosphite, and the content of the surfactant in the composition is from 0.01 to 5 wt.% (see US Pat. 02 published 04/27/2003).

The disadvantages of the substance according to the method include the complexity of the production technology and the lack of data on the possibility of using it for the treatment of dermatitis in cats.

Known substance according to the method for the treatment of wounds and burns, which includes the following components, wt. %: colloidal silver - 0.0001-0.1, colloidal zinc oxide - 5-15, glycerin - 5-15, methylcellulose - 0.05-5, distilled water - the rest. EFFECT: invention provides acceleration of tissue regeneration processes of burn wounds, as well as wound healing, as a reparative, anti-inflammatory and microcirculation-improving agent for the treatment of thermal, solar and chemical burns (see US Pat. 17/02, published 11/23/2017).

The disadvantage of this substance according to the method is the indications for the treatment of wounds and burns and the lack of indications for the treatment of dermatitis of various etiologies in cats.

The closest in technical essence and achieved positive result, adopted by the authors as a prototype, is the active substances colloidal silver and colloidal zinc oxide (see US Pat. ).

The disadvantage of using colloidal silver and colloidal zinc oxide is the indications for the treatment of wounds and burns and the lack of indications for the treatment of dermatitis of various etiologies in cats.

Disclosure of invention

The objective of the invention is to develop a drug with a highly effective regenerating, wound healing effect, made in the form of a solution for the treatment of dermatitis of various etiologies in cats.

The technical result that can be achieved using the present invention is to reduce the time and increase the effectiveness of the treatment of dermatitis of various etiologies in cats.

The technical result is achieved using a drug for the treatment of cats with dermatitis of various etiologies, including colloidal silver, zinc oxide, glycerin, methylcellulose and distilled water, characterized in that it additionally contains the amino acid salt of lanthanum and glycerin with the following ratio of components in wt. %:

Colloidal silver 0.0001-0.1 Colloidal zinc oxide 5-15 Lanthanum amino acid salt 5-15 Methylcellulose 0.05-0.5 Glycerol 5-15 Distilled water rest

The technical result is achieved through the use in the treatment of cat dermatitis of various etiologies of a pharmaceutical hydrophilic composition, which includes methylcellulose as a structure-forming agent and a hydrophilic base of the entire composition, colloidal particles of silver, zinc oxide of the amino acid salt of lanthanum as the main active ingredients, glycerin as a moisture-retaining component and distilled water, while the presence of colloidal silver in the composition of the pharmaceutical hydrophilic ointment composition is due to its high bactericidal, fungicidal and antiviral properties, the use of colloidal particles of zinc oxide as the main active component of the pharmaceutical hydrophilic ointment composition is associated with their anti-inflammatory, drying, adsorbing, astringent and antiseptic effect (bactericidal and fungicidal properties). And, as you know, zinc oxide is a source of zinc ions, which, by saturating the tissues of the wound surface, further stimulate regeneration processes. Organic lanthanum compounds potentiate anti-inflammatory, bactericidal and wound healing effects in the composition. Lanthanum compounds have osmotic activity (drying effect, reduction of edema), accelerate the repair and healing of the skin, have an antipruritic effect, which is especially important in the treatment of cat dermatitis.

Drawings and other materials

The figure 1 shows the effectiveness of the drug according to example 1, depending on the change in the area of the skin defect,%.

The figure 2 shows the effectiveness of the drug according to example 2, depending on the change in the area of the skin defect,%.

The figure 3 shows the effectiveness of the drug according to example 3, depending on the change in the area of the skin defect,%.

The figure 4 shows the effectiveness of the ratios of components in wt.% depending on the regression of clinical signs of the disease.

The figure 5 shows the effectiveness of the ratios of components in wt.%, depending on the change in the area of the skin defect, %

The figure 6 demonstrates the effectiveness of therapy in cats with dermatitis of various etiologies, depending on the ratio of the components in wt.%

Implementation of the invention

In the conditions of a veterinary clinic, the developed drug was tested. The drug was applied to 32 cats with dermatitis of various etiologies with skin damage. For research formed 4 groups of 8 animals each: 1 group, treatment using the composition according to example 1; group 2 - treatment using the composition according to example 2; group 3 - treatment using the composition according to example 3; group 4 - treatment using the prototype.

Example 1. The developed drug is a suspension of white color with a yellowish tint, odorless, homogeneous in consistency (has no mechanical inclusions). The essence of obtaining a drug for the treatment of dermatitis is as follows. Methylcellulose brand MTs-100 is mixed with distilled water and left to swell for 30-60 minutes. Then, colloidal zinc oxide (ZnO), glycerin, colloidal silver and the amino acid salt of lanthanum La(AK)3xnH ₂ O are added to the formed methylcellulose gel with constant stirring, followed by physicochemical treatment to prevent the processes of destabilization and aggregation of colloidal particles of zinc oxide (ZnO) , silver (Ag) and the amino acid salt of lanthanum La (AK) 3xnH ₂ O.

For the treatment of the first group of cats, the following ratio of components is used, wt.%:

Colloidal silver <0.0001 Colloidal zinc oxide 1-5 Lanthanum amino acid salt 1-5 Methylcellulose 0.01-0.4 Glycerol 0.1-5 Distilled water rest

It is established that this ratio of components, wt. % to be effective in 50% of cases, namely 4 cats were cured after the use of the drug (see Fig. 6). The duration of the treatment course is 18.2 days (see Fig. 6). On the 3rd and 5th day the following clinical signs were registered: edema, erythema and exudation (see Fig. 4). Changes in the area of the skin defect on the 3rd day - 10.2±1.4%, on the 5th day - 10.8±1.8%, on the 7th day - 12.4±1.2% (see Fig. 5), therefore, the ratio of the components is not optimal (see figure 1) for the treatment of dermatitis of various etiologies in cats.

Example 2. Carried out analogously to example 1, but take the following ratio of components in wt. %:

Colloidal silver 0.0001-0.1 Colloidal zinc oxide 5-15 Lanthanum amino acid salt 5-15 Methylcellulose 0.05-5.0 Glycerol 5-15 Distilled water rest

It is established that this ratio of components, wt. % to be effective in 88% of cases, namely 7 cats were cured after the use of the drug (see Fig. 6). The duration of the treatment course is 8.1 days (see Fig. 6). On days 3 and 5, no clinical signs were recorded, namely edema, erythema and exudation (see Fig. 4). Changes in the area of the skin defect on day 3 - 28.6 ± 1.2%, on day 5 - 28.6 ± 1.2%, on day 7 - 32.4 ± 2.5% (see Fig. 5), therefore, the ratio of the components is optimal (see figure 2) for the treatment of dermatitis of various etiologies in cats.

Example 3. Carry out analogously to examples 1 and 2, but take the following ratio of components in wt. %:

Colloidal silver 0.2-1.0 Colloidal zinc oxide 15-30 Lanthanum amino acid salt 15-30 Methylcellulose 5.1-10.0 Glycerol 16-20 Distilled water rest

It is established that this ratio of components, wt. % to be effective in 63% of cases, namely 5 cats were cured after the use of the drug (see Fig. 6). The duration of the treatment course is 16.3 days (see Fig. 6). On the 3rd and 5th day the following clinical signs were registered: edema, erythema and exudation (see Fig. 4). Changes in the area of the skin defect on the 3rd day - 11.5±1.7%, on the 5th day - 14.2±1.1%, on the 7th day - 16.8±1.7% (see Fig. 5), therefore, the ratio of the components is not optimal (see figure 3) for the treatment of dermatitis of various etiologies in cats.

Example 4. Determination of the irritant and allergic effect of a drug with a highly effective regenerating, wound-healing effect, made in the form of a solution for the treatment of dermatitis of various etiologies in cats, was carried out by the method of skin and conjunctival tests. We studied the irritant and allergic effect of the drug for the treatment of dermatitis of various etiologies in cats on laboratory rabbits of the California White breed (Belenky, M. L. Elements of a quantitative assessment of the pharmacological effect / M. L. Belenky. - 2nd ed. revised. add. - Leningrad: Medical Literature, 1963. - 162 pp. 17. Belenky, ML Methods for determining the toxicity and danger of chemicals / ML Belenky, 1970. - 71 pp.).

On the basis of the Department of Therapy and Pharmacology of the Stavropol State Agrarian University, a study of the drug for the treatment of dermatitis of various etiologies in cats was carried out, applying it to the clipped areas of the lateral surface of the skin of rabbits for 14 days 5 times a week, using a daily exposure of 4 hours, after which the drug for treatment of dermatitis of various etiologies in cats was washed off with water. The skin reaction was assessed using the Suvorov scale. With a negative result, a second study was carried out, the experiment was continued and the number of applications was increased to 20 for 20 days (Belenky, M. L. Elements of a quantitative assessment of the pharmacological effect / M. L. Belenky. - 2nd ed. revised. add. - Leningrad: Medical Literature, 1963. - 162 pp. 17. Belenky, ML Methods for determining the toxicity and danger of chemicals / ML Belenky, 1970. - 71 pp.).

The study of irritant action was investigated using the "conjunctival test" on 10 rabbits. Rabbits in the conjunctival sac of the right eye were instilled with 1 drop of the drug for the treatment of dermatitis of various etiologies in cats. The other eye of the rabbit was used as a control; a sterile isotonic NaCl solution was instilled into it. Animals were observed for 7 days.

According to the results of the study of the irritant and allergic effect of the drug, which has a highly effective regenerating, wound-healing effect, made in the form of a solution for the treatment of dermatitis of various etiologies in cats, no reddening of the skin, thickening of the skin fold and loss of trimmed wool and wool bordering on clipped areas were noted. On palpation of the shorn skin areas, no painful reaction was observed in animals. There was no development of itching, acute ophthalmitis, thinning of the corneal epithelium, swelling of blood vessels, and conjunctival edema.

Thus, the most optimal for the treatment of cats with dermatitis of various etiologies is the following ratio of components in wt. %:

Colloidal silver 0.0001-0.1 Colloidal zinc oxide 5-15 Lanthanum amino acid salt 5-15 Methylcellulose 0.05-0.5 Glycerol 5-15 Distilled water rest

Thus, the created drug according to this invention meets all the medical and biological requirements for the creation of modern medicines for the treatment of burn wounds:

1. Contains active ingredients that have reparative, wound healing, anti-inflammatory and microcirculation-improving properties.

2. Creates an optimal microenvironment for the healing of skin lesions.

3. It has a high adsorption capacity for wound exudate.

4. Does not have a local irritant and allergic effect.

5.Easy to apply to the surface of the skin; easy to use for veterinarians.

Claims (2)

A preparation for the treatment of cats with allergic dermatitis and viral dermatosis, including colloidal silver, zinc oxide, glycerin, methylcellulose and distilled water, characterized in that it additionally contains the amino acid salt of lanthanum and glycerin in the following ratio of components in wt. %: Colloidal silver 0.0001-0.1 Colloidal zinc oxide 5-15 Lanthanum amino acid salt 5-15 Methylcellulose 0.05-0.5 Glycerol 5-15 Distilled water rest

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