RU2757053C1 - Method for protecting basalt-plastic reinforcements from fungal damage - Google Patents

Abstract

FIELD: coatings.SUBSTANCE: invention relates to methods for protecting building polymer structures, in particular, basalt-plastic reinforcements, from fungal damage. Proposed is a method for protecting basalt-plastic reinforcements from fungal damage, consisting in treatment thereof with an epoxy polymer composition based on a binder, a filler and an accelerator for resin hardening, wherein epoxy diane resin of the ED-22 brand is used as a binder, 2,-4,-6-Tris/dimethylamine/-methyl/-phenol (UP-606/2) is used as an accelerator for epoxy resin hardening, and zeolite powder from the Khonguruu field with a fraction of 0.02 to 0.05 mm, whereon a mixture of Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D taken in an equal ratio is immobilised, is used as a filler, wherein the components are taken in the following ratio, % wt.: binder 81.0; accelerator 9.0; filler 10.0.EFFECT: method increases the stability of basalt-plastic reinforcements in a mycological environment and can be used to protect basalt-plastic reinforcements from fungal damage.1 cl, 5 dwg, 16 tbl, 4 ex

Description

The invention relates to methods for protecting building polymer structures, in particular basalt-plastic reinforcement (BPA), from fungal attack. A method for protecting BPA from fungal infection is proposed, which consists in processing BPA with an epoxy polymer composition, including a binder, a filler and an accelerator for curing the resin, characterized in that an epoxy-diane resin of the ED-22 brand (GOST R 56211-2014) is used as a binder; as a filler, a modified preparation "Bacillosorboil" (TU 2164-001-52483924-2012); for curing epoxy resin, 2, -4, -6-Tris / dimethylamino / -methyl / -phenol (UP-606/2) (TU 2494-630-11131395-2006) is used; with the following ratio of components, wt. %: binder - 81.0; accelerator - 9.0; filler - 10.0.

The proposed solution can be used to protect polymer composite materials (PCM), in particular BPA, from fungal infections.

The advantage of the proposed method is that it is simple to execute and economical, since it does not require complex technological equipment and expensive components for its implementation, and the resulting protective epoxy-polymer composition used in the method increases the stability of BPA in a mycological environment and can be used for protection of BPA from fungal infection.

State of the art.

Currently, more than 50% of the total volume of registered damage to polymer materials in the world is associated with the activity of microorganisms (1. Dergunova AV Biodamage of structures of buildings, structures and assessment of damage from biological damage [Text] / AV Dergunova, V.T. Erofeev, VF Smirnov // Defects of buildings and structures. Strengthening of building structures: materials of the XIV scientific-method. Conference VITU - SPb. - 2010. - pp. 175-179).

As agents of biological damage, microorganisms cause irreversible changes in the structural and functional properties of materials, which affects the reduction in the operation of products. Such an effect is usually called biodamage (2. Pekhtasheva E.L. Biodamage and protection of non-food products: Textbook for students of higher educational institution / Under the editorship of A.N. Neverov. - M .: Masterstvo, 2002. - S. 3).

Most often, biodeterioration of polymer composites is associated with the effect of microscopic fungi on them. The mold fungi of the genera: Alternaria, Aspergillus, Chaetomium, Cladosporium, Mucor, Fusarium, Penicillium, Rhizopus, Scopulariopsis, Trichoderma (3. Sukhrevich VI Protection against biological damage caused by fungi [Text] / VI Sukharevich, I.L. Kuzikova, N.G. Medvedeva // SPb: ELBI-SPB. - 2009. - 207 p.).

Under favorable conditions (temperature, humidity, pH, etc.), the fungal mycelium is able to penetrate into microcracks, accumulate its biomass there, as a result of which the cracks expand and branch, and already, in the course of their life, the fungi release metabolic products, the effect of which on materials is the main cause of biodamage and deterioration of their operational properties.

In this regard, the study of bioinfection of polymer composite materials (PCM) by microscopic fungi is carried out in parallel with the study of the influence of environmental factors on the processes of damage caused by this group of microorganisms and methods of their inactivation and protection of polymer composites from fungal infection. This is necessary for the development of an effective application of technologies aimed at the long-term operation of the PCM.

The solution to the problems of corrosion and bio-damage in the construction industry has been found in the use of basalt fiber, which is made from natural volcanic rock formed in the form of basalt lavas. However, despite the strength and reliability of basalt, a number of researchers have established the susceptibility of basalt fiber and materials based on it to damage.

So, a group of authors considered the processes of corrosion of basalt fiber in various environments and found that under the action of an alkaline medium, destruction of the fiber can occur (4. Manushina A.S., Urbanov A.V., Zyryanov M.S., Sapronov A.O. , Potapova E.N. Corrosion of basalt fiber in a gypsum binder environment // Advances in chemistry and chemical technology, volume XXXI, No. 1, 2017. - 3 p.).

Other scientists have found that the kinetic curves of changes in the breaking load of basalt and some glass rovings during their alkaline corrosion can be both monotonically decreasing and have local extrema (5. Dalinkevich A.A., Buldakov V.P., Gumargalieva K.Z. , Marakhovsky SS, Sukhanov AV Kinetics of alkaline corrosion of basalt fibers // Corrosion: materials, protection, No. 2, 2012. - pp. 33-41).

In the study of the resistance of basalt fibers to oxidation and the effects of an acidic environment and high temperature, researchers have shown that an acidic environment and high-temperature processing of fibers lead to their mechanical destruction (6. Garshev A.V., Knotko A.V., Pulkin M.N. ., Zemtsov A.N., Gramenitskiy E.N., Ivanov V.K., Putlyaev V.I., Tretyakov Yu.D. Oxidative corrosion of basalt fiber // Corrosion: materials, protection, No. 7, 2005. - P . 33-39).

Considering that the mycelium of fungi can use for its development very thin cracks that have appeared as a result of mechanical destruction from the influence of abiotic and biotic factors, the problem of protecting composites based on basalt fiber from fungal infection is urgent, especially in modern conditions, when various objects are widely introduced composite rebar made of basalt fiber.

Thus, the destruction of PCM, including those made from basalt fiber, depends not only on the structure of the materials themselves, but also on environmental conditions that contribute to the growth of certain microbial populations (7. Erofeevskaya L.A., Neustroeva N.I. , Kychkin A.K., Kychkin A.A. Investigation of the effect of microorganisms on the structure and properties of polymer composite materials in a cold climate // Scientific developments: Eurasian region: materials of the international scientific conference of theoretical and applied developments (Moscow, 15.11. ) / editor-in-chief D.R.Khismatullin. - Moscow: Publishing house of Infinity, 2018.

Despite the fact that the composition of microorganisms capable of causing biological damage to BPA is not as diverse as, for example, biodestructors of plant objects, the danger of their contamination with microflora is obvious, and the search for ways to prevent and combat biological damage is relevant and in demand, in general, as for other PCM. ...

Currently, there are two main approaches to combating biodamage of PCM, the first of which is the prevention of contamination and infection with microflora involved in the biodegradation of objects and the second is based on inactivation and destruction of microorganisms that have managed to cause infection and damage to materials or their components. For these purposes, disinfectants, antiseptic, bactericidal, fungicidal and protective agents of various compositions are most often used.

So, for example, halogen-containing preparations of a wide spectrum of antimicrobial action are known: "Deactive-chlorine" (8. TU9392-018-44454660-2010); Medichlor (9. TU 9392-010-60437111-2011); Samarovka (10. TU 9392-002-52798823-00); "Deo-chlorine" (11. Guidelines for the use of the disinfectant "Deo-chlorine" Yekaterinburg, approved by the Department of the State Sanitary and Epidemiological Supervision of the Ministry of Health of Russia No. 14-3 / 355-09 of 27.12.2002); "Chlormix" (12. Instructions for the use of the disinfectant "Chlormix" No. 103 dated 2009, manufactured by "Gidrakhsm Ltd", Great Britain); "Purzhavel" (13. Instructions for the use of "Purzhavel" disinfectant No. 1 of 2007, manufactured by "ARCH WATER PRODUKTS"), "Chloramine B" (14. TU 9392-031-00203306-2003); "Whiteness-3" (grades A and B) (15. TU 9392-409-05763458-2007); Domestos (16. TU 2386-010-18359701-2001); "Aqua-chlorine" (17. Instructions for the use of the disinfectant "Aqua-chlorine" No. 1/08 of 2008, manufactured by PKF "West" LLC, Russia); "Clorsept-17" (18. Instructions for the use of disinfectant "Clorsept" No. 25/08 of 2008, manufactured by LLC "Samarovo", Russia); "Javellon" (19. Instructions for the use of the disinfectant "Javellon / NoveltyChlor" No. 1/07 (manufacturer "Est. Linosier", France), approved by LLC "RusBio" dated 29.12.2006).

The disadvantage is high aggressiveness to structural materials, the ability to form environmentally hazardous compounds, sensitivity to the action of inorganic and organic substances, temperature, light, pH activity, in addition, the funds are not used as a protective agent for BPA against fungal infection.

Known aldehyde-containing funds "Klindzin-Forte" (20. Instructions for the use of disinfectant "Klindazin-Forte" No. 2 of 2003 (manufactured by Metrex Research LLS, USA); "Klindzin-3000", (21. 3000 "No. 18/11 of 2011, manufactured by LLC" Hygiena Plus ", Russia);" NU-Sideks "(22. Instructions for the use of disinfectant" NU-Sideks "No. 07/2012 dated 2021, manufactured by LLC" Hygiena Plus ", Russia);" Dyulbac DTBL "(23. Instruction No. 8 for the use of Dyulpak DTBL (Dyulbac maxi) by PFH SNS (FFC CNC), France, for disinfection and pre-sterilization treatment, approved by RASTER LLC from 02/11/2004); Septodor Forte (24. Guidelines for the use and quality control methods of the disinfectant Septodor Forte (Happy Day-M LLC, Russia, approved by the Department of State Epidemiological Supervision of the Ministry of Health of Russia No. 11-3 / 129- 09 from 11.04.2001).

The disadvantage is the corrosiveness to the composite material, increased volatility and the associated need to treat surfaces with solutions of high concentration, in addition, the listed known agents are not used as a protective agent for BPA against fungal infection.

Known means "Katril-Dez" based on peracetic acid (25. TU 9392-211-10968286-2009 with changes No. 1.2).

The disadvantage is the corrosiveness to various materials and the fact that the agent is not used as a protective agent against fungal infection for BPA.

Known disinfectants based on haamines "Baktizilin" (26. TU 9392-015-18885462-2005), quaternary ammonium compounds (QAC) "Deo-anti-mold" (27. TU 2386-004-26433370-2003), "Lizoformin special" (28. Instructions for the use of the disinfectant "Lizoformin special" (company "Lizoform Dr. Hans Rosemann GmbH, Germany) in medical institutions, approved by OOO" Lizoform SPb ", 05.06.2004)," Socrena "(29. Instructions for the use of Socren's disinfectant No. 03/06/07 of 2007, manufactured by BODE Chemie GmbH, Germany); "Septabik-20 V" (30. Technological instruction for the use of the disinfectant "Septabik" of the firm "Abik" (Israel) for disinfection of equipment and communications at enterprises for the production of drinks, approved by the Technical Committee for Standardization 91 "Beer, non-alcoholic and wine products, 03.03.1999); Antiplesin (31. TU 2386-003-70001225-2003), Desofran (32. TU 9392-001-46483209-2004), Lizoformin special (33. TU 9392-024-00479095-98), phenols Kemi Side (34. TU 9392-001-56715159-04), Amotsid (35. TU 939028-00479095-98), TeflexA (36. TU 9392-008-23170704-2007).

The disadvantages of the above disinfectants are the ability to cause corrosion of materials, inactivity to pathogenic fungi, reaction to the presence of various organic substances, in addition, the listed known agents are not used as a protective agent for BPA against fungal infection.

Known antiseptic agents "Biopag-D" (37. TU 9392-020-31547288-02), "Phosphopag" (38. TU 9392-007-415447288-99), "Polisept" (39. TU 9392-001-32963622- 99) based on polyhexamethylguanidines.

The disadvantages of these antiseptic agents are the reasons listed above.

It is known to use dialkyl ketone peroxide as a biocide (40. Patent RU No. 2316355. The use of dialkyl ketone peroxide as a biocide: sterilizing, antiseptic, disinfecting and antiparasitic agent. C2, IPC A61L 2/18, A61K 9/14, A61L 101/22, C02F 1/72. Application 2005135461/15 from 2004.04.16. Published on 2008.02.10. Authors: Coronado Luengo Alonso (ES), Randus García Juan José (ES). Patentee: Neochemical Desarrollos Avanzados, SA (ES)).

The disadvantage is the corrosiveness of peroxides and the fact that the known solution cannot be used for BPA as a protective agent against fungal attack.

There is a known substance called prochloraz (N-propyl-N- [2- (2,4,6-trichloro-phenoxy) ethyl] imidazole-1-carboxamide) under Chemical Abstract registration number 67747-09-5, which is a broad spectrum fungicide actions.

The disadvantage of the known fungicide is that it is used for plant protection and its possibility and method of application as a protective agent of BPA against fungal infection have not been studied. In addition, independent studies have found that prochlorase, in general, exhibits insufficient action against fungi (41. Drysdale J.A. et all. New Zeland Journal of Forestry Science, 1982, 12/3, P. 457-466).

Known antiseptic liquid (42. RF patent No. 2146611. Antiseptic liquid for wood impregnation ZhTK-2 (its variants). C1, IPC В27К 3/50. Application 99108057/04, 1999.04.15. Published: 2000.03.20. Authors: Dolmatov L.V., Kutukov I.E., Akhmetov A.F., Sukhorukov AM, Khanilo V.I., Nikolaychuk V.A., Abdrakhmanov PP Patentee: Ufa State Petroleum Technological University), including the product of processing of petroleum aromatic hydrocarbons, polyolefins, paraffinic hydrocarbons, catalytic cracking oil fractions boiling in the range of 325-400 ° C, as well as a catalytic cracking fraction boiling in the 205-325 ° C range.

The disadvantage of this antiseptic is the complicated technological process due to the multicomponent composition, the multistage preparation of the components before adding them to the composition, the need to use high temperatures (up to 400 ° C) technology, which requires additional equipment, in addition, the well-known antiseptic was tested only for wood impregnation and the possibility and method of its use as a protective agent for BPA against fungal infection is not known.

A known method of producing an antiseptic (43. RF patent No. 2197375. A method of obtaining an antiseptic for impregnating wood. C1, IPC В27К 3/52. Application 2001119796/04, 2001.07.16. Published: 2003.01.27. Authors: Klimenkov OM, Negoda L.L., Shitikov D.A.Patentee: Samara State Academy of Architecture and Civil Engineering), which consists in the use of an impregnating antiseptic composition in the form of a water-soluble biological product that contains diammonium phosphate, carbamide, sodium fluoride and acetone-formaldehyde resin.

The disadvantages of the known composition is that it is not used as a protective agent for BPA against fungal infection, but is tested only for the impregnation of wood, in addition, the antiseptic is quickly washed out even from wood materials.

Thus, one of the important disadvantages of most of the above listed antiseptic, disinfecting and fungicidal agents is their corrosiveness and the fact that they are not used as a protective agent for BPA against fungal infection.

In modern times, various protective polymer coatings have been developed and introduced into industry and the national economy. So, there is a known method of protection against biofouling based on a powder polymer coating, which includes: metallic zinc, zinc oxide and titanium oxide (44. Patent US No. 6974847. Melt compounded fusion bonded marine antifouling coating. B1, С08K 3/08, С08К 3/22, C08L 63/100, C09D 5/16. Patent Submission Date 2002.07.16. Patent Grant Date 2005.12.13. Patent Inventor: Jacky T. Thygesen. Patent Applicant: Matrix Engineering).

The disadvantage of this method is the complication of the technological process due to the need to use high temperatures (up to 300 ° C) in the technology, which requires additional equipment, in addition, the possibility and method of its use to protect the BPA from fungal infection has not been studied.

Known epoxy polymer solution, including epoxy dianovy resin ED-20, polyethylene polyamine PEPA, plasticizer - waste epoxy resins (mother epoxy resin MSE-1 grade B), filler - expanded clay production waste (45. Patent RU 2248950. Epoxy polymer solution. IPC C08V11 18/18, С04В 18/20, С04В 26/14, C08L 63/02, C09D 163/02. Application 2003132021/04 dated 31.10.2003. Published 27.03.2005. Authors: Voronkov A.G., Yartsev A. P. Patentee: TSTU (RU)). The known polymer solution has a low cost, satisfactory physical and technical characteristics, environmental safety.

The disadvantage of the known polymer solution is that it is not used as a protective agent for BPA against fungal infection.

Known epoxy-phenolic polymer solution, in the composition, part by weight: epoxy resin ED-20 - 100, phenolic mixture - 100, hardener polyethylene polyamine (PEPA) - 10, filler (ground quartz sand) - 400 (46. Sokolova Yu.A. Modified epoxy adhesives and coatings in construction / Yu.A. Sokolova, EM Gotlib. - M .: Stroyizdat, 1990. - S. 130-150).

The disadvantage of the known solution is insufficient stability in a mycological environment and the fact that it is not used as a protective agent for BPA against fungal infection.

Known especially heavy protective polymer solution, including epoxy dianovy resin ED-20, polyethylene polyamine PEPA, organosilicon varnish KO-922, mineral filler, alloying additive (47. Patent RU 2119899. Particularly heavy polymer solution. C1, IPC С04В 26/14. Application 93027622/14. Application 93027622 / 04 of 1993.05.18 Publ. 1998.10.10 Authors: Proshin A.P., Solomatov V.I., Khudyakov V.A.Patentee: Penza State Institute of Architecture and Civil Engineering).

The disadvantage of the proposed epoxy polymer solution is that it is designed to protect against gamma radiation and is not used to protect PCM, in particular BPA from fungal infection.

Known protective especially heavy polymer solution, including epoxy dianic resin ED-20, polyethylene polyamine PEPA, organosilicon varnish KO-916K, mineral filler - organic glass waste based on lead silicates (48. Patent RU 2125975. Extra heavy polymer solution. C1, IPC С04В 26/14 , С04В 24/14, С04В 14/22. Application 94004183/04 from 1994.02.08. Publ. 1999.02.10. Authors: Proshin A.P., Solomatov V.I., Khudyakov V.A., Kozlov V.A. Patentee: Penza Civil Engineering Institute).

The disadvantage of the proposed epoxy polymer solution is that it is designed to protect against ionizing radiation and is not used to protect PCM, in particular BPA from fungal infection.

Known nanomodified polymer composite, including epoxy resin ED-20, polyethylene polyamine PEPA, silicone varnish KO-922, titanium dioxide with a specific surface area of 6000 m ² / kg and ground quartz sand with a specific surface area of 200 m ² / kg as a filler, quartz sand fractions 0.63 ... 1.25 as filler (49. Patent RU 2488563. Nanomodified polymer composite. C1, IPC С04В 26/14, С04В 14/06, В02В 1/00. Application 2012127433/04 dated 2012.07.02. Publ 2013.07.27 Authors: Smirnov V.A., Korolev E.V. Patentee: FSBI VPO "MGSU"), which can be used for the manufacture of elements of enclosing structures intended for operation under conditions of exposure to atmospheric moisture, solar radiation and cyclic temperature measurements.

The disadvantage of the known nanomodified polymer composite is that it is not used to protect PCM, in particular, BPA from fungal infection.

A polymer solution is used as a prototype, including, in parts by weight: epoxy resin ED-20 - 100, hardener polyethylene polyamine (PEPA) - 10 ... 15, filler (waste from the production of chemical glass polishing based on calcium fluoride, modified with a solution of butyl rubber in gasoline) - 100 ... 110 (50. Patent RU 2022943. Polymer solution. C1, IPC С04В 26/14, С04В 14/28, С04В 24/12. Application 4929493/05 from 1991.04.22. Publ. 1994.11.15. Authors: Proshin A. P., Solomatov V.I., Beloborodov A.V.Patentee: Penza Civil Engineering Institute). The proposed polymer solution allows you to protect structures from water corrosion.

The disadvantage of the known solution is that the polymer solution is not used to protect PCM, in particular BPA, from fungal infection.

Thus, despite a fairly wide variety of disinfecting, antimicrobial, fungicidal and protective polymer-composite agents for combating destruction and biodeterioration of materials, there is still a need in the prior art to develop methods for protecting PCM, in particular BPA, from fungal infection.

The objective of the invention is to expand the range of methods for protecting PCM, in particular BPA, from fungal infections.

The problem is solved by the fact that a new method of protecting BPA from fungal infection was obtained, which consists in processing BPA with a protective epoxy-polymer composition, including a binder, a filler and an accelerator for curing the resin, which differs from the prototype in that epoxy-diane resin of the brand is used as a binder. ED-22 (51. GOST R 56211-2014 Uncured enoxide-diane resins. Specifications. OKS 83.080.10. OKP 22 2511. Date of introduction: 2016.01.01); as a filler modified preparation "Batsillosorboil" (52. TU 2164-001-52483924-2012 Preparation "Batsillosorboil" for cleaning soil from contamination. Date of introduction: 2012.01.13. Listed in the register of FBU "Rostest-Moscow" 2012.03.15 under No. 200/077067; 53. Expert opinion No. 500 for compliance with the Unified Sanitary-Epidemiological and Hygienic Requirements for Goods Subject to Sanitary-Epidemiological Supervision (Control). Registration number 1267 dated 03.26.2013) for curing epoxy resin 2, -4, - 6-Tris / dimethylamino / -methyl / -phenol (UP-606/2) (54. TU 2494-630-11131395-2006 Accelerator UP-606/2 for epoxy resins; 55. Sanitary and epidemiological conclusion No. 78.22.01.249. P.000051.12.06 dated 18.12.06); with the following ratio of components, wt. %: binder - 81.0; accelerator - 9.0; filler - 10.0.

The proposed method for protecting BPA from fungal infection expands the range of methods for protecting PCM, in particular BPA, from fungal infections and can be used to protect PCM, in particular BPA, from fungal infections.

In addition, the proposed method has additional qualities, namely, simplicity of execution and cost-effectiveness, because for its implementation does not require complex technological equipment and expensive components, and the resulting protective epoxy-polymer composition for processing BPA contains the drug "Bacillosorboil" as a filler. based on natural zeolite, which is both an affordable and a cheap raw material, since zeolites are surface-deposited and are mined in an open pit.

About 1000 large clinoptilolite deposits have been discovered on the globe. More than 50 of them were found in the CIS and more than 20 in Siberia. Among them: Khongurinskoye - in Yakutia; Kholinskoye and Shivyrtuiskoye - in the Chita region; Pegasskoye - in the Kemerovo region; Nazarovskoe - in the Krasnoyarsk Territory; Radenskoe - in the Jewish Autonomous Region; Chuguevskoe - in the Far East, etc. (56. Burov A.I. The main regularities of the distribution of zeolite deposits in Russia // Actual problems of the development of zeolite raw materials from the Khonguruu deposit / Materials of scientific readings dedicated to the memory of the discoverer of the deposit K.E. Kolodeznikov. - Yakutsk: Izd. -vo YANTs SB RAS, 2005. - p. 26-28) (table 1).

A review of the available literary sources shows that zeolites of various deposits are not inferior to each other in physical and mechanical properties and macro- and microelement composition (57. Gerasimova V.N. Natural zeolites as adsorbents of petroleum products // Chemistry for sustainable development, 2003, vol. 11, No. 3, pp. 481-488] (tables 2; 3).

In the proposed method of protecting BPA from fungal infection, a modified preparation "Bacillosorboil" is used as a filler for the polymer composition, the basis of which is the zeolite-containing rock of the Khonguruu deposit (Suntarsky district, Western Yakutia) with a zeolite content of 70-98% and with a chemical composition: SiO ₂ 65 , 55-67.31%; Al ₂ O ₃ 11.73-12.33%; Fe ₂ O ₃ 0.75-1.00%; MgO 1.13-1.81%; CaO 1.81-3.18%; Na ₂ O 0.74-2.75%; K ₂ O 1.08-1.62%; H ₂ O 10.86-12.43%. (table 4).

Zeolite of the Khonguruu deposit (Western Yakutia) is a frame aluminosilicate, the crystal lattice of which is formed by tetraids with a well-developed inner surface, this provides adhesion to microbial cells, allowing microorganisms to be fixed not only on the surface, but also inside the carrier, which serves as a protection for microbial cells from mechanical damage and adverse environmental conditions.

One gram of zeolite corresponds to an inner surface of 800 m ² . Hence it follows that the density of zeolites is also low and amounts to 1.9-2.3 g / cm ³ (58. Shadrin AM Natural zeolites in animal husbandry, veterinary medicine and environmental protection. - Novosibirsk, 1986. - 116 p.).

Earlier studies on the use of zeolite from the Honguruu field as a carrier for microorganisms showed good results when used in biological products for bioremediation of oil-contaminated lands in the oil and gas complex of Yakutia (59. Patent RU No. 2565549 Biological product for bioremediation of oil-contaminated soils for natural climatic conditions C2, MCP V09S 1/10, B01J 20/16, C09K 17/40, C12N 11/14, C12N 1/20, C12R 1/07, C12R 1/425; 2013155969/10. Appl. 17.12.2013. Publ. . 20.10.2015, bulletin No. 29. Authors: Erofeevskaya L.A., Glyaznetsova Y.S. O.G., Glyaznetsova Yu.S., Erofeevskaya L.A., Nikolaeva A.V. Methods of bioremediation of oil-contaminated soils for climatic conditions of the Far North and assessment of their effectiveness // Science and technology of pipeline transport of oil and oil products. No.1 (17) , 2015 - S. 90-97).

Environmental safety, a wide raw material base and a relatively low cost (100-200 times cheaper than subtitles) of natural zeolite determine its widespread use in various areas of the national economy: in animal husbandry, agriculture, construction industry, cosmetology, medicine, ecology.

The use of zeolite-containing raw materials as a filler of a protective polymer composition for BPA is promising, since, unlike organic fillers, which can serve as a substrate for biodestructors and microorganisms that reduce the fungal resistance of the material, zeolite, on the contrary, increases biostability and is a natural fungicide and can serve as an additive. increasing the bactericidal capacity of the compositions, as evidenced by the studies of the authors of the method for increasing the action of the disinfectant, in which the zeolite of the Khongurinsky deposit (hongurin) is used as an additive at the rate of 10%, which allows maintaining the activity of the active substance by 71% and increasing the bactericidal activity up to 5 times (61. Patent RU No. 2230038. A method for increasing the effect of a disinfectant. C1, IPC C02F 1/50, A61 / L 2/16, application 2003114289/15, 2003.05.14, publ. 2004.06.10. Authors: Neustroev MP, Tarabukina N.P., Prokopyeva N.I. Patent holders: Yakutsk Research Institute here agriculture SB RAAS (RU)).

Tests on the use of zeolite from the Honguruu deposit, immobilized by bacterial strains with fungicidal activity to protect BPA from fungal infection, were carried out for the first time.

In this technical solution, bacterial cultures for the drug "Bacillosorboil", which serves in the method as a filler for a protective epoxy-polymer composition, were selected from the collection of microorganisms of the Institute of Natural Sciences of the Siberian Branch of the Russian Academy of Sciences (Yakutsk, Avtodorozhnaya st., 20).

In total, 24 collection strains from the working collection of the Institute of Petroleum Geophysics of the Siberian Branch of the Russian Academy of Sciences were investigated. For further testing, Bacillus simplex B-2817D and Bacillus sp. B-2815D, which are part of the drug "Bacillosorboil", in which a targeted study revealed new properties, in particular, antagonistic activity against molds of the genus Aspergillus (A. niger and A. fumigatus), previously isolated from a fragment of basalt-plastic reinforcement, exhibited at the climatic testing range of the Institute of Physical Problems of the Siberian Branch of the Russian Academy of Sciences (Yakutsk, Avtodorozhnaya st., 20).

The strains that make up the modified preparation "Bacillosorboil" were deposited in the All-Russian Collection of Microorganisms (VKM) of the Federal State Budgetary Institution of Science Institute of Biochemistry and Physiology of Microorganisms named after V.I. G.K. Scriabin of the Russian Academy of Sciences (IBFM RAS) with the assignment of registration numbers: Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D.

Previously, the strains were used as destructors of oil and petroleum products (62. RF patent No. 2675941 The bacterial strain Bacillus simplex VKM B-2817D is a destructor of oil and petroleum products. Application No. 2017139339. Date of receipt 13.11.2017 Incoming No. 068579. Application published 15.11.2017 A positive decision on the grant of a patent on October 26, 2018. The date of commencement of the patent is 11/13/17 Published on 12/25/18, Bulletin No. 36. Author: Erofeevskaya LA Copyright holder of the Federal Research Center "YSC SB RAS"; 63. RF patent No. 2675940 Bacillus sp.VKM B-2815D bacterial strain - oil and petroleum product destructor Application No. 2017139337 Date of receipt 13.11.2017 Incoming No. 068577 Application published 15.11.2017 Positive decision for the grant of a patent 26.10.2018 Date of patent validity 13.11.17 Published on 25.12.18, Bulletin No. 36. Author: Erofeevskaya LA .. Copyright holder of the Federal Research Center "YSC SB RAS"), antagonistic activity of Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D, which are part of the drug "Bacillosorboil" in relation to molds isolated from BPA fragments, was established for the first time by the method of targeted research.

Screening of antagonist strains was carried out according to the principle of selection of microbial cultures with fungicidal activity against pathogenic molds of the genus Aspergillus (A. niger and A. fumigatus (copyright PKI-12)), previously isolated from BPA fragments exhibited at the IPTP climatic testing ground SB RAS (Yakutsk, Avtodorozhnaya str., 20) (64. Erofeevskya LA, Aleksandrov AR, Kychkin A.K. Prospects for the Use of Spore-forming Bacteria to Combat the Destruction of Polymeric Composite Materials // International science and technology conference " FarEastCon-2019 "IOP Conf. Series: Materials Science and Engineering753 (2020) 052010 IOP Publishing - 2020. doi: 10.1088 / 1757-899X / 753/5/052010).

To study the antagonistic activity of the selected strains, we used the methods generally accepted in microbiology:

1. The method of perpendicular strokes, based on inoculation with a bacteriological loop of the studied culture of the bacterial strain with a stroke on the surface of industrial mesopotamia agar (MPA) frozen in a Petri dish. Sowing is cultivated at a temperature favorable for the growth of the test culture (in this technical solution: + 28 ° C) for 24-72 hours for diffusion in MPA of inhibitory compounds formed during the cultivation of the test strain. Then, in compliance with the rules of asepsis, perpendicularly from the edge of the Petri dish, the culture of the test strain (in this technical solution: A. niger or A. fumigatus), previously isolated from BPA fragments, is carefully streaked to the stroke of the grown culture. Only one culture is sown on one Petri dish; for another culture, use another dish with a previously prepared culture of the bacterial strain under study. Then the crops are cultivated at a temperature of +25 for five days. The presence of antagonistic activity in the culture of the studied bacterial strain is judged by the presence of a zone of inhibition of the test strain. By the size of the diameter of the zone of inhibition of the test strain, the degree of antagonistic activity of the studied culture of the bacterial strain is judged.

2. The method of drops, which consists in applying on the surface of the dried MPA a drop of the studied culture of the bacterial strain, previously grown in mesopatamia broth (MPB). A drop of the studied strain applied to the surface of the agar is left at room temperature until the drop is completely absorbed. Then, in compliance with the rules of asepsis, retreating 1-2 mm from the edge of the first spot of the dried drop, apply a drop of the second test culture. Spreading, the drop with the second culture enters the spot with the first culture by about half the diameter. In the superimposed part of the culture during co-cultivation, the test strains of microorganisms develop in competition with each other. At the same time, the free parts of the spots of each culture applied to the MPA serve as a control of the viability of each of the cultures and the germination of the nutrient medium. After the second drop of the test culture has dried, the Petri dishes with the inoculations are placed in a thermostat with the lid down and cultured at a temperature of + 28 ° C. The test result of cultures of microbial strains is taken into account by the presence (absence) of visually visible signs of suppression of the growth of one culture by another.

3. Medod of agar blocks, which consists in sowing a test culture of a microorganism on a Petri dish with MPA and cultivation at + 28 ° C for 24-72 hours until inhibitory compounds are formed and accumulated in agar. Then, in compliance with the rules of asepsis, an agar block with a grown culture is cut out with a sterile bacteriological spoon or cork drill and placed in another Petri dish with MPA, on the surface of which the culture of the test strain is preliminarily inoculated (in this technical solution: A. niger PKI-12 or A. fumigatus PKI-12). Crops are cultivated at a temperature of +25 for five days. The degree of antagonistic activity of the test culture of the microorganism is judged by the size of the zone of inhibition of the growth of the test strain around the agar block.

4. The method of holes, which consists in surface sowing of the test strain on the surface of the MPA. In compliance with the rules of asepsis with a Drygalski spatula, we prepare the lawn by rubbing the test culture into agar. After that, a hole with a diameter of 5-7 mm is cut out with a sterile bacteriological spoon or a cork drill, which is filled with the culture liquid of the strain under study. Petri dishes are cultivated in a thermostat at a predetermined temperature for the required time for the growth of the test strain, after which the zone of inhibition of the test strain around the well is measured and, if necessary, the index of antagonistic activity is calculated.

More details about methods for determining the antagonistic activity of bacteria can be found in the General Pharmacopoeia Monograph (65. OFS.1.7.2.0009.15 Determination of the specific activity of probiotics).

The results of testing the selected strains for antagonistic activity are presented in table 5.

It was found that when testing the relationship between antagonist strains and mold cultures that are sensitive to them, isolated from fragments of basalt-plastic reinforcement by the method of agar blocks, the antagonistic activity is higher when using a pair composition of cultures {Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D) than when using antagonist strains in monocultures.

The zone of growth inhibition when using a paired composition of Bacillus sp. VKM B-2815D + B. simplex VKM B-2817D, taken in a 1: 1 ratio with a concentration of 1-9 10 ⁶ cells / ml in relation to a mixture of mold cultures as part of A. niger PKI-12 + A. fumigatus PKI-12, pre-isolated from fragments of BPA and taken in a 1: 1 ratio with a concentration of 1-9 10 ⁶ cells / ml was 53 mm.

When using a monoculture of Bacillus sp. VKM B-2815D with a concentration of 1-9 10 ⁶ cells / ml against a mixture of mold cultures in A. niger PKI-12 + A. fumigatus PKI-12, previously isolated from BPA fragments and taken in a 1: 1 ratio with a concentration of 1 -9 10 ⁶ cells / ml, the growth inhibition zone was 24 mm.

When using monoculture B. simplex VKM B-2817D with a concentration of 1-9 10 ⁶ cells / ml against a mixture of mold cultures in A. niger PKI-12 + A. fumigatus PKI-12, previously isolated from BPA fragments and taken in the ratio 1: 1 with a concentration of 1-9 10 ⁶ cells / ml, the zone of inhibition of mold growth was 26 mm.

Thus, it was found that the antibacterial activity of the paired culture in the Bacillus sp. VKM V-2815D + V. simplex VKM B-2817D, taken in a 1: 1 ratio with a concentration of 1-9 10 ⁶ cells / ml, works against a mixture of mold cultures as part of A. niger PKI-12 + A. fumigatus PKI-12, previously isolated from BPA fragments and taken in a 1: 1 ratio with a concentration of 1-9 10 ⁶ cells / ml according to the principle of cooperation and synergy.

The performed studies served as the basis for obtaining a method for protecting BPA from fungal infection, which is illustrated by the following examples.

Example 1. Preparation of basalt-plastic reinforcement

At the initial stage of testing, BPA samples were prepared, which are unidirectional basalt-plastic rods with a periodic profile with a diameter of 20 mm.

BPA samples were manufactured by TBM LLC on the Struna technical line in accordance with TU (66. TU 2296-001-86166796-2013 "Non-metallic composite reinforcement made of basalt plastic").

For the manufacture of BPA, an initial binder was used, the basis of which is ED-22 epoxy resin, cured with iso-methyltetrahydrophthalic anhydride (iso-MTHFA) (67. TU 6-09-124-91 Hardener iso-MTHFA) in the presence of an accelerator 2.4 , 6-tris (dimethylaminomethyl) phenol (UP-606/2) (table 6).

The main characteristics of this epoxy resin are presented in table 7.

The main characteristics of iso-MTHFA are shown in Table 8.

Since the curing of epoxy resins with anhydrides is extremely slow, accelerators are usually introduced into these systems. The choice of 2,4,6-tris (dimethylaminomethyl) phenol (UP 606/2) as an accelerator is due to its high catalytic activity associated with the presence of three tertiary amino groups and acidic phenolic hydroxyl in this compound (see Figure 1 - 2.4 , 6-tris (dimethylaminomethyl) phenol).

However, it manifests itself at rather high temperatures (above 100 ° C).

At processing temperatures, the processability of the binder in the presence of an accelerator is retained.

The characteristics of the curing accelerator UP-606/2 are presented in Table 9.

The physical and mechanical characteristics of basalt roving used in the technology are shown in Table 10.

For tests on fungal resistance, samples were prepared from BPA fragments in three versions: basalt-plastic rods 20 cm high (sample No. 1); discs 0.5 cm high, 2 cm in diameter, obtained by horizontal cut of basalt-plastic rods (sample No. 2); fragments of basalt-plastic rods 20 cm high, 0.5 cm wide, obtained by a vertical cut (sample No. 3).

The BPA samples were treated in one layer with a protective epoxy-polymer composition obtained according to the method described below, after which the tests for resistance to mold infestation were started.

Example 2. Obtaining an epoxy-plastic composition and method of its use for protecting BPA from fungal infection

The method of protecting BPA from fungal infection consists in treating the reinforcement with an epoxy polymer composition, where, as a binder, epoxy-diane resin of the ED-22 brand (GOST R 56211-2014) is used; as a filler, a modified preparation "Bacillosorboil" (TU 2164-001-52483924-2012); for epoxy resin curing, 2, -4, -6-Tris / dimethylamino / -methyl / -phenol (UP-606/2) (TU 2494-630-11131395-2006) is used.

Modification of the preparation "Bacillosorboil" was carried out by mechanical treatment of zeolite from the Khonguruu deposit, which serves as a carrier for microorganisms in the preparation, to a fraction of 0.02-0.05 mm, followed by immobilization of the biomass of two selected bacterial strains on it (Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D), which were previously tested for antagonistic activity using the methods described above.

Mechanical treatment of the zeolite was carried out on a vibration mill in fractional mode with breaks for 1 minute of grinding.

After grinding, the resulting zeolite powder was sterilized and hydrophobized at a temperature of + 160 ° C in a ShS-80M drying oven to constant weight.

At the next stage, the powder was cooled at room temperature and immobilized with a previously obtained mixture of a microbial suspension of bacteria in the composition: Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D.

Before the production and immobilization of the biomass of bacteria on the zeolite-containing raw material prepared by the above method, the main morpho-cultural and physiological-biochemical characteristics and fungicidal activity of the selected strains were re-determined (Table 11).

To obtain a microbial mixture for the immobilization of zeolite raw materials, bacterial cells Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D, from the beveled MPA were washed off with a 0.9% NaCl solution, the initial microbial suspensions of bacterial isolates were prepared, separately for each strain, with a volume of 25 cm ³ , with a concentration of 1⋅10 ⁹ microbial cells / cm ³ according to the optical standard GISK im. AM Tarasevich.

The resulting initial microbial suspensions were mixed in an equal ratio (1: 1). The resulting mixture of microbial suspension immobilized zeolite powder prepared by the above method in the ratio, wt. %: zeolite powder - 91; biomass of bacteria - 9.

Immobilization of bacteria on zeolite powder was carried out by contact method at room temperature for 48 hours, after which it was dried at room temperature for 48-72 hours.

Next, the fungicidal activity of the already modified drug "Bacillosorboil" was determined using the method of activating the drug in a mesopatamia broth (MPB) of industrial production.

To do this, 1 gram of the drug "Bacillosorboil" was mixed in 9 cm ³ MPB, the test tube was shaken for 5-10 seconds and placed in a thermostat for 72 hours at a temperature of plus 37 ° C. After that, the mixture was filtered under aseptic conditions using sterile filters and laboratory glassware. The resulting bacillary filtrate was mixed with molten sterile agar, in an equal volume (3 cm ^{3 of the} filtrate and 3 cm ^{3 of} molten agar) and quickly mixed. The resulting agar mixture was mowed in a biological test tube with a volume of 9-10 cm ³ (tube diameter 16 mm). After the agar solidified, the tested fungal cultures were inoculated onto its surface. The inoculation was cultivated in a thermostat at a temperature of + 25 ° C for 5 days. The lack of growth on the agar surface indicated the presence of fungicidal activity of the filtrate.

The filler prepared by the above described method was mixed with epoxy diane resin ED-22 in the ratio, wt. %: epoxy resin - 81; filler - 10 and stirred for 2-4 minutes, after which, to accelerate the hardening of the resin, an accelerator was added - 9% by weight and mixed again for 1-2 minutes, a protective epoxy-polymer composition was obtained for application on BPA as a protective agent from fungal infection.

The total preparation time for the protective epoxy-polymer composition is 5-6 minutes. The resulting epoxy-polymer composition was applied to the BPA with a mackerel brush immediately after preparation, since the polymer composition begins to thicken already 5 minutes after the introduction of the accelerator. After 20 minutes, the BPA can already be taken with your hands (with gloves). Full hardening occurs in 24 hours.

Example 3. Test of basalt-plastic reinforcement after treatment with a protective epoxy-polymer composition for the effect of mold fungi

Testing of prototypes of BPA, after treatment with a protective epoxy-polymer composition, for exposure to mold fungi was carried out in accordance with the approved methodology (68. GOST R 57859-2017 Polymer composites. Test methods for the effects of molds).

Before the test, preparation for the experiments was carried out:

1. Developed a map of the experiments;

2. Prepared laboratory room for testing;

3. Sterilized the required set of laboratory glassware;

4. According to example 1, prepared prototypes for testing;

5. Prepared prototypes of BPA were treated with a protective epoxy-polymer composition obtained in example 2;

6. Prepared agar mineral medium of the following composition (g / l): KH ₂ PO ₄ - 0.7; MgSO ₄ × 7H ₂ O - 0.7; NH ₄ NO ₃ - 0.1; NaCl - 0.5; FeSO _{4 x7H 2} O - 0.002; ZnSO ₄ × 7H ₂ O 0.002; MnSO ₄ × H ₂ O 0.001; agar 15.0; water is the rest. The composition was sterilized for 20 min in an autoclave at a temperature of 121 ° C, after which the acidity (pH) of the medium was adjusted to 6.0-6.5.

Then, from the working collection, test cultures of microscopic fungi (A. niger PKI-12 and A. fumigatus PKI-12) were selected, previously isolated from BPA samples exhibited at the climatic testing ground in Yakutsk.

On the basis of the selected strains, a consortium was artificially created to infect the prototypes and the nutrient mineral medium with fungal spores.

Then, sterile melted agarized mineral medium prepared in advance according to the recipe of point 6 was poured into sterile Petri dishes and sterile trays for testing large samples until the thickness of the medium layer was approximately 6 mm. After the nutrient medium had solidified, prototypes of BPA prepared according to example 1 were placed on its surface, contaminating the surface of the medium and the surface of the test samples themselves, obtained by the mushroom suspension using a sterile spray bottle.

After that, the inoculations were closed and placed with their lids up in a thermostat. Incubation was carried out at a temperature of plus 28 ° C and a relative humidity of 85-90% for 30 days (from April 29 to May 28, 2019).

The effect of molds on BPA samples was assessed visually with the naked eye and under a microscope.

We used a laboratory polarized microscope Axiolab Pol, manufactured by "Carl Zeise" Germany, an N-Achromat 5 × / 0.13 objective. Numerical aperture × 1000. Maximum useful microscope magnification - 130,000 and biological microscope "Biomed-3" with magnification from 40 to 1000 times.

The results were evaluated on a 4-point scale in accordance with the data in Table 12.

Experiments have shown that the obtained epoxy polymer composition increases the resistance of BPA to fungal infection, as evidenced by the test results below (Table 13; Figures 2-5).

Figure 2 shows a method of sowing on the surface of a nutrient agarized medium, a native prototype obtained by the method of horizontal cutting of a basalt-plastic reinforcement rod, not treated with a protective epoxy-polymer composition in order to isolate cultures of microorganisms that contaminated the prototype before the experiment, from the air environment of an industrial premises and equipment on which the reinforcement was cut into discs.

Figure 3 shows an overgrowth of the native prototype No. 4 (see Table 13) of a fragment of basalt-plastic reinforcement obtained by a horizontal cut and not treated with a protective epoxy-polymer composition, 15 days after the start of incubation in a thermostat at a temperature of plus 28 degrees Celsius.

Figure 4 shows an overgrowth of the native prototype No. 4 (see Table 13) of a fragment of basalt-plastic reinforcement, obtained by a horizontal cut and not treated with a protective epoxy-polymer composition, 20 days after the start of incubation in a thermostat at a temperature of plus 28 degrees Celsius.

Figure 5 shows an overgrowth of the native prototype No. 4 (see Table 13) of a fragment of basalt-plastic reinforcement obtained by a horizontal cut and not treated with a protective epoxy-polymer composition, 30 days after the start of incubation in a thermostat at a temperature of plus 28 degrees Celsius.

Example 4. Test of basalt-plastic reinforcement after treatment with a protective epoxy-polymer composition for exposure to molds in an aqueous environment

Testing of prototypes of basalt-plastic reinforcement rods (BPA), after treatment with a protective epoxy-polymer composition, for exposure to molds in an aqueous medium was carried out under laboratory conditions for three months (from January 13 to March 12, 2020).

Sterile BPA samples were tested.

Sterilization of the BPA prototypes was carried out in a steam sterilizer (autoclave) of the DGM-200 brand (Switzerland) at a temperature of plus 126 ° C for 30 minutes.

The experiments were exposed in thermostats of the TS-1/80 SPU brand at a constant temperature (+ 28 ° C) and a relative humidity of 100%.

Experiment options:

1. Sterile BPA samples coated with a protective epoxy-polymer composition obtained according to examples 1 and 2 were immersed in purified (fresh) drinking water contaminated with a mixture of spores of fungi A. niger PKI-12 + A. fumigatus PKI-12, taken in equal ratio (1: 1).

2. Sterile BPA samples coated with a protective epoxy-polymer composition obtained according to examples 1 and 2 were immersed in salt water containing 3.5% NaCl, infected with a mixture of spores of fungi A. niger PKI-12 + A. fumigatus PKI-12 taken in an equal ratio (1: 1).

3. As a control, we used sterile samples of fittings immersed in purified drinking and salt water without sowing mushroom suspensions containing spores of the tested mold fungi and BPA samples not treated with a protective epoxy-polymer composition, immersed in fresh water and saline solution contaminated with mold spores. mushrooms.

Each experiment was placed in three replicates in trays until the samples were completely immersed in water contaminated with mold spores.

It was found by experiments that the obtained epoxy polymer composition increases the resistance of BPA to fungal infection, both in a fresh mycological environment and in a saline solution contaminated with mold spores, as evidenced by the following test results (Table 14).

Example 4. Test of basalt-plastic reinforcement after treatment with a protective epoxy-polymer composition for exposure to molds in a soil environment infected with mold spores

The testing of BPA, after treatment with a protective epoxy-polymer composition, for exposure to molds in the soil environment was carried out for six months under the conditions of a field experiment (from May 15 to October 14, 2019) and under laboratory conditions (from September 11 to March 13 2020).

Experiment options:

1. Sterile BPA samples (rods and discs) coated with a protective epoxy-polymer composition obtained according to examples 1 and 2 were wrapped in cotton cloth previously infected with a mixture of spores of fungi A. niger PKI-12 + A. fumigatus PKI-12 obtained in the above described way. Convolutions with BPA samples were immersed in light loamy soil moistened by irrigation to a depth of 20 cm.The test samples were exposed for 6 months in the open air under natural conditions or under laboratory conditions in thermostats at a constant temperature (plus 28 ° C) and humidity (80-90% ).

2. Non-sterile BPA samples (rods and disks) coated with a protective epoxy-polymer composition obtained according to examples 1 and 2, were wrapped in cotton cloth previously infected with a mixture of fungi spores A. niger PKI-12 + A. fumigatus PKI-12 obtained in the above described way. Convolutions with BPA samples were immersed in light loamy soil moistened by irrigation to a depth of 20 cm.The test samples were exposed for 6 months in the open air under natural conditions or under laboratory conditions in thermostats at a constant temperature (plus 28 ° C) and humidity (80-90% ).

Experiments have found that the resulting epoxy polymer composition increases the resistance of BPA to fungal attack in the soil, as evidenced by the below test results (Tables 15-16).

Thus, it has been shown that the proposed method of protecting BPA from fungal infection, in comparison with the prototype, makes it possible to increase the resistance to damage to the reinforcement by mold fungi, both in water (fresh and salty) and in the soil substrate, as in laboratory conditions, when exposed to constant temperature and humidity of the environment (OS), and under the conditions of a field experiment, under the influence of changing natural conditions of the environment (air temperature, precipitation, atmospheric pressure, solar radiation).

The advantage of the proposed method is that it has simplicity of execution and cost-effectiveness, since for its implementation it does not require complex technological equipment and expensive components, and the resulting protective epoxy-polymer composition proposed in the method increases the stability of the material in a mycological environment and can be used for protection of BPA from fungal infection.

Claims (1)

A method of protecting basalt-plastic reinforcement from fungal infection, including the treatment of basalt-plastic reinforcement with an epoxy polymer composition based on a binder, filler and accelerator for resin curing, characterized in that ED-22 epoxy-diane resin is used as a binder, as an accelerator for epoxy resin curing 2, -4, -6-Tris / dimethylamino / -methyl / -phenol (UP-606/2) is used, and zeolite powder from the Honguruu deposit with a fraction of 0.02-0.05 mm is used as a filler, on which the mixture is immobilized bacteria Bacillus sp. VKM B-2815D and Bacillus simplex VKM B-2817D, taken in an equal ratio, and the components are taken in the following ratio, wt%: binder - 81.0; accelerator - 9.0; filler - 10.0.

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