RU2732230C1 - Method of rooted stock material vsl-2 in vitro - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a method of producing rooted stock material VSL-2 in vitro, comprising cutting and seeding of one-stem grafts on agarised nutrient medium containing macro- and microelements and vitamins based on Murashige and Skoog nutrient medium recipe, Fe-chelate, agar-agar, saccharose, after which sprouts are transplanted into a medium with high concentration of salts required for stimulation of root formation of plants, which are systematised, depending on chemical properties, into separate mother solutions (Chelate-Fe 20 mg/l, KNO₃ 2000 and CaCl₂ - 1500 mg/l), where large-size shoots are used for rooting, and smaller ones are transplanted for reproduction (content 6 Bap 2.0 mg/l).

EFFECT: when using the described method, the output of the VSL-2 root material in vitro is 85–90 % average.

1 cl, 1 tbl

Description

The invention relates to the field of agriculture, in particular to gardening. The method includes grafting test-tube plants and planting single-node cuttings on an agar nutrient medium containing macro- and microelements, and vitamins based on the prescription of the Murashige and Skoog nutrient medium, Fe-chelate, agar-agar, sucrose, the following salt concentration is added to the medium Chelate - Fe 20 mg / l, KNO3 - 2000 and CaCl2 - 1500 mg / l, necessary to stimulate plant root formation.

There is a known method of selecting the composition of nutrient media based on the salts of the nutrient medium Murashige and Skoog (1962) MS for introduction into culture and for clonal micropropagation of vegetatively propagated rootstocks and the optimal height of microshoots within 1.3-2.0 cm. modified medium based on the basic composition of the nutrient medium of Murashige and Skoog (1962) for most of the tested clonal rootstocks of stone fruit cultures, allowing the rooting of 40 to 89% of the total number of microcuttings (https://pdfs.semanticscholar.org/4bf7/4427f47ef0704c4e2afa9098af02a7283)

The invention relates to the field of agriculture, in particular to gardening. The method includes grafting test-tube plants and planting single-node cuttings on an agar nutrient medium containing macro- and microelements and vitamins based on the prescription of the Murashige and Skoog nutrient medium, Fe-chelate, agar-agar, sucrose, the following salt concentration Chelate is added to the medium - Fe 20 mg / l, KNO3 - 2000 and CaCl2 - 1500 mg / l, necessary to stimulate rooting of plants, which are systematized, depending on chemical properties, into separate mother solutions, where large shoots are used for rooting, and the smaller ones are transplanted for reproduction (with a content of 6 Bap2.0 mg / l).

The invention is implemented as follows: for the stock material VSL - 2, the content of cytokinins at the first stage is taken from 0.2 to 0.25 mg / l, auxins from 0.01 to 0.1 mg / l, after which the resulting shoots are transplanted onto a nutrient medium Murashige Skoog with a high concentration of salts necessary to stimulate root formation of plants (Chelate - Fe 20 mg / l and replace KNO3 2000 mg / l with CaCl2 - 1500 mg / l), the resulting shoots of large size (5-6 cm) are used for rooting , and the smaller one for reproduction by transplanting onto a nutrient medium with a content of 2.0 mg / l 6 Bap. Table 1.

As a result of the research, the most acceptable nutrient media based on nutrient agar media from Murashige and Skoog (1962) and Woody Plant Media for the rooting stage were determined.

Depending on the genetic origin of the rootstock, the rooting rate of explants when introduced into the culture on the above-mentioned modified media is, on average, not less than 85%: it was highest in the rootstocks of VSL 2. When using the described method, the yield of rooted rootstock VSL-2 in vitro on average 85-90%.

Claims (1)

A method for obtaining rooted rootstock material VSL-2 in vitro, in which the cuttings of test tube plants are planted and single-node cuttings are planted on an agar nutrient medium Murashige Skoha containing cytokinins from 0.2 to 0.25 mg / l, auxins from 0.01 to 0, 1 mg / l, after which the resulting shoots are transplanted onto the Murashige Skoog nutrient medium with a higher concentration of salts necessary to stimulate the rooting of plants (Chelate - Fe 20 mg / l and replace KNO3 2000 mg / l with CaCl2 - 1500 mg / l), obtained shoots of a larger size (5-6 cm) are used for rooting, and smaller ones for reproduction, transplanted onto a nutrient medium containing 2.0 mg / l 6 BAP.