RU2681791C1 - Biologically active addition of protective action “dreamfood” - Google Patents

Abstract

FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology, namely to biologically active additives (BAA). Biologically active additive of the protective action is characterized by the fact that it is a denucleinized bacterial biomass of the strain of methanol-oxidizing bacteria Methylococcus capsulatus GBS-15. Invention allows to obtain dietary supplements with a high protein content as the main component of sports nutrition products that enhance the protective functions of the body of athletes.EFFECT: use of dietary supplements also maintains a state of high physical and mental performance in extreme conditions.1 cl, 3 tbl, 1 ex

Description

The invention relates to microbiology and biotechnology, in particular to biologically active additives (BAA), in particular to the product DREAMFOOD and can be used as food additives with a high protein content as the main component of sports nutrition products that enhance the protective functions of the body for athletes, conducting therapeutic and preventive nutrition of people working in areas of environmental disasters in extreme conditions.

Scientific research in the field of determining both the physiological effect of certain ingredients of food products and their technological functions is relevant and timely (the Government of the Russian Federation approved on October 25, 2010 the “Fundamentals of the state policy of the Russian Federation in the field of healthy nutrition for the period until 2020” (No. 1873-p).

A promising direction for optimizing the nutrition of the population and, above all, that part of it that experiences great physical, psycho-emotional, and mental stress (for example, military personnel, athletes, miners, foundry workers, etc.), is the inclusion of foods with a high content in the diet biologically active substances (BAS), preferably of natural origin from plant-protein raw materials.

A large number of biologically active food additives are known to enhance the protective functions of the human body.

To date, no one disputes the fact that sports muscle overload and the state of excessive stress are inseparable (Zimin Yu.I., 1979; Kassil G.R. et al., 1978). It is considered proven that any stress is inevitably accompanied by damage to internal organs working at the limit of their functionality [Meerson F.Z. Adaptation, stress and prevention. M .: Science. - 1981. - 278 p.]. It is known that athletes (swimmers) have high titers of autoantibodies to the tissues of their own organs (liver, kidneys, heart) more than five times the frequency of such findings in beginners healthy athletes.

Biologically active substances from silt rocks and sapropelic mud are known. The obtained compounds have biological activity, exhibit hepatoprotective effect (AC. No. 1260005). The disadvantage of this substance is that it contains fat-containing fractions, therefore, it has a limited range of applications.

It is known a tool that improves performance, tolerance of extreme loads, adaptability to extreme conditions, containing dry yeast, including those used in brewing, processed before their lysis, with plant extracts or an antioxidant (RF No. 2390271). Brewer's yeast and products of their processing have a complete set of essential amino acids, minerals and vitamins, which makes them an excellent restorative complex, but lacking adaptogenic and hepatoprotective properties, in addition, they have a specific aftertaste.

Probiotics and medical nutrition products are widely used abroad based on consortia of bifidobacteria strains of the species B. bifidum, B. longum, B. adolescentis, B. breve, B.infantis in various combinations (US Patent No. 5843922, US Application No. 20052015990; US patent No. 6335040), as well as on the basis of consortia of strains of bifidobacteria and lactobacilli (US Application No. 2002090416; US patent No. 5866385).

Various bacterial strains that are protein producers have found widespread use as a microbial protein mass. So, technologies have been developed for the production of feed protein based on waste from distillery (post-alcohol stillage) and flour (bran, flour) industries and a mixture of stillage with waste from grain raw materials (RF patents No. 2159287, 2140449, 2054881; USSR copyright certificates No. 1595900, 1571061; 1532580; 1507787 and others). As producer cultures in the above inventions, yeast-like imperfect fungi of the genus Candida are used. A disadvantage of known strains of microorganisms for the production of dietary supplements is the lack of ability to actively grow in a long non-sterile cultivation process.

Also, various bacterial strains that are protein producers have found application as a microbial protein mass: Saccharomycescerevisiae yeast strain (Ed. St. USSR N 1575570); Lactobacillusacidophilus strain (RF No. 2244000); Lactobacillusplantarum strain (RF No. 2390554, 2244001); Propionibacteriumfreudenreichiisubsp strain. shermanii (RF No. 2247149), strain Propionibacteriumiumacnes (RF No. 2250258); Acetobacter methylicum strain of acidophilic methylotrophic bacteria (ed. St. USSR No. 925112); strain Flammulina velutipes (auth. certificate. USSR No. 727687); strain Pleurotus ostreatus Kummer (ed. St. USSR No. 427993). A common disadvantage of the above bacteria is the unstable yield of biomass, the insufficiently high productivity of the strain.

There are various strains of bacteria producing feed protein related to different types of methane-oxidizing bacteria (methanotrophs), for example, strains of the genus Methylococcus capsulatus CMPM B-1743 (ed. Certificate of the USSR No. 770200), Pseudomonas methanica, Methylococcus capsulatus BKM B-2116 Methylocystis parvus BKM B-2129, Methylosinus sporium BKM B-2123, Methylosinus trichospohum BKM B-2117, Methylobacter acidophilus, Methylomonas rubra VSB-90, Methylococcus sp. FM-9, Methylococcus capsulatus VSB-874, Methylococcus minimus, Methylomonas methanica, Methylomonas agile.

They are the products of microbial synthesis obtained by culturing methane-oxidizing bacteria on natural gas. These strains are characterized by different growth rates and biomass yields.

The closest in technical essence and the achieved result is a strain of Methylococcus capsulatus GBS-15, which is a producer of protein mass, which can be used for animal feeding (RF No. 2613365). - producer of biomass.

The objective of the invention is to expand the arsenal of biologically active products for sports nutrition that increase the functional reserves of the body, resistance to stress, contributing to the rapid restoration of strength after physical exertion and the psycho-emotional state of athletes on the background of taking the product under the working name "DREAMFOOD".

The technical result of the claimed invention is the creation of a natural protein product from which nucleic acids are isolated. The proposed dietary supplement has a high energy potential, provides high efficiency when included in the diet in conditions of prolonged and increasing in intensity limiting muscle overloads. The use of the drug maintains a state of high physical and mental performance in extreme conditions.

The stated technical problem is achieved through the use of a biologically active additive of a protective effect, characterized in that a bacterial biomass using a methane-oxidizing bacteria strain Methylococcus capsulatus GBS-15, from which nucleic acids are isolated.

The need for proteins for the human body is quite high due to the high rate of their metabolism in tissues. It is estimated at about 240 grams of protein per day. The actual need for food protein is determined by the availability of essential and non-essential amino acids, which are required to maintain the normal nutritional status of an adult, pregnant women, athletes, people with diseases of various nature, as well as for the normal growth and development of children of different ages. With a lack of complete protein in food, the body goes into a state of catabolism, using its own proteins as a source of nitrogen. To avoid this phenomenon, timely provision of the body with proteins or essential and non-essential amino acids is necessary. Of practical importance are not native proteins, but denatured ones that are better absorbed by the human body.

When ingested with food, the nucleic components contained in the bacterial biomass exert an additional load on the excretory system, as in the process of metabolism are processed into salts of uric acid, capable of accumulation in the body. Preliminary isolation of nucleic components from protein increases the nutritional value of products, minimizing the formation and deposition of salts of the components of the decay of nucleic acids. The isolated and purified nucleic acids themselves can be used to obtain immunomodulating and regenerating drugs.

The characteristics of the feedstock for obtaining the claimed product are shown in Table 1. The characteristics of the denucleinated biomass are shown in Table 2. The DREAMFOOD product is obtained by extraction of native cells of methane-oxidizing bacteria from biomass, then nucleic acids — DNA and RNA — are isolated from the obtained extract. The content of protein substances in the finished product is 60-80 mass%.

According to the results of calculations, the organization of the release of a protein product of various degrees of purification in modern biochemical plants can significantly increase the profitability of the production of feed microbial protein due to the implementation of more expensive products. Nucleic acids isolated from the protein mass will be commercial products for manufacturers used in medicine and cosmetology, immunomodulators and regenerative agents.

The effect of the bioprotective effect of the product "DREAMFOOD" is confirmed by tests conducted at the Scientific Research Institute of Medical Problems of Sports.

The results of the tests showed that the product "DREAMFOOD" has a pronounced normalizing effect on the basic systems of the human body, provides stability when working in extreme conditions. A statistically significant increase in stability was obtained when determining static physical endurance by an average of 30-40%. Tests of the impact of the product "DREAMFOOD" on the human body under extreme conditions showed that the product helps maintain a state of physical performance at a higher level, increases the activity of tissue respiration enzymes. The use of the product "DREAMFOOD" in the nutrition of athletes contributed to a reduction in the timing of adaptation to meteorological factors, an increase in general immunity was observed, and normalization of the functions of the gastrointestinal tract took place.

Disclosure of invention

Example 1. Studies of the effectiveness of supplements to the normal diet of athletes-boxers of the product "DREAMFOOD" were conducted on the basis of the specialized boxing school for the Olympic reserve for children and youth.

Athletes of the main group (15 people) took the product "DREAMFOOD" - 2 tablespoons 2 times a day with meals for 40 days. The control group consisted of 14 athletes with a normal diet.

The observation results showed an improvement in the subjective state of the athletes of the main group by an average of 80%. The positive effect was expressed in improving overall health, reducing symptoms of asthenia, normalizing sleep.

The results of the assessment of the psychophysiological status after the health-improving course revealed an increase in the self-esteem score for athletes of the main group from 5.17 to 5.69 (an average of 10.1%).

In general, these results indicate a positive effect of the product "DREAMFOOD" on the psycho-emotional state of athletes in the main group.

In the comparative assessment of strength indicators by the method of hand dynamometry in the main group while taking the product "DREAMFOOD", there was a tendency to increase the strength indicator of both the right and left hands compared to the control group.

In the main group, after taking the “DREAMFOOD” product, the duration of breath holding increased by an average of 3.8 seconds (19.8%), at the same time, in the control group, with initially higher average values of breath holding, a decrease in the duration of breath holding was noted 1.7 seconds on average (7.8%).

Pulse recovery in the main group before the rehabilitation program was observed in 74.4% of cases, after the course of recovery - in 86.6% of cases (increased by 12.2%). In the control group, pulse recovery was observed at the beginning of the observation in 78.6% of cases and at the end of the observation in 64.3% (decreased by 14.3%).

Analysis of blood pressure indicators in the main group showed that at the beginning of the observation, the level of systolic blood pressure recovered in 80% of athletes, after a rehabilitation program - in 93.3% of athletes. In the control group, indicators remained unchanged.

The dynamics of recovery indicators of systolic blood pressure in the main group after the rehabilitation program improved by an average of 13.3%. In the control group, indicators remained unchanged.

The data presented indicate a favorable effect of the reception of the product "DREAMFOOD" on the recovery rates of systolic and diastolic blood pressure after exercise, while in the control group there was no positive dynamics in the level of diastolic blood pressure.

Based on the results obtained, the following conclusions are made:

1. Enrichment of the diet with the product "DREAMFOOD" helps to improve the functional status of the cardiovascular and respiratory systems in boxing athletes according to the results of exercise tests.

2. The identified tendency to increase strength indicators according to the results of wrist dynamometry indicates an increase in muscle strength.

3. The use of the product “DREAMFOOD” contributes to the normalization of indicators of vegetative status, which has a positive effect on endurance, including an increase in the intensity and duration of physical activity during competitions and training.

4. Enrichment of the diet with the product “DREAMFOOD” leads to positive changes during the adaptation processes and, thus, helps to increase the reserves of adaptation of the body of athletes to increased stress and physical stress.

The experimental results are presented in tables 3-6.

The experiment clearly showed that the use of the protein product "DREAMFOOD" helps to improve the functional status of athletes-wrestlers. A tendency to increase strength indicators in control exercises after applying this product was identified, which allows to optimize the intensity of the training process in athletes. The revealed significant increase in indicators in strength exercises indicates an increase in strength preparedness.

Thus, the product “DREAMFOOD” can be effectively used as a functional nutrition product in the athletes ’diet, ensuring the intake of substances necessary for maintaining the body’s energy balance under conditions of increased physical and emotional stress, for increasing muscle strength and endurance, for faster adaptation to physical activity and recovery of strength after training. With regular use, the product has a positive effect on the functional state of the cardiovascular, respiratory, autonomic systems, psychological and emotional state of a person.

The product can also be recommended for a balanced diet of people seeking a healthy lifestyle, for therapeutic and preventive nutrition at the stages of rehabilitation after various diseases.

Claims (1)

A biologically active additive of a protective effect, characterized in that it is a denucleinated bacterial biomass of the methane-oxidizing bacteria strain Methylococcus capsulatus GBS-15.