RU2639508C2 - Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке - Google Patents

Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке Download PDF

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RU2639508C2
RU2639508C2 RU2014120422A RU2014120422A RU2639508C2 RU 2639508 C2 RU2639508 C2 RU 2639508C2 RU 2014120422 A RU2014120422 A RU 2014120422A RU 2014120422 A RU2014120422 A RU 2014120422A RU 2639508 C2 RU2639508 C2 RU 2639508C2
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probe
fad
aforementioned
gene
canola
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RU2014120422A
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RU2014120422A (ru
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Ласанта Чандана УБАЯСЕНА
Зое ЭЛЕРТ
Чандра Шекара А. ЧАННАБАСАВАРАДХЯ
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ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
RU2014120422A 2011-10-21 2012-10-19 Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке RU2639508C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161550165P 2011-10-21 2011-10-21
US61/550,165 2011-10-21
PCT/US2012/060998 WO2013059576A1 (en) 2011-10-21 2012-10-19 Method to determine zygosity of the fad2 gene in canola using end-point pcr

Related Child Applications (1)

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RU2017143585A Division RU2017143585A (ru) 2011-10-21 2012-10-19 Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке

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RU2014120422A RU2014120422A (ru) 2015-11-27
RU2639508C2 true RU2639508C2 (ru) 2017-12-21

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RU2014120422A RU2639508C2 (ru) 2011-10-21 2012-10-19 Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке
RU2017143585A RU2017143585A (ru) 2011-10-21 2012-10-19 Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке

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RU2017143585A RU2017143585A (ru) 2011-10-21 2012-10-19 Способ определения зиготности гена fad-2 канолы с использованием пцр с детекцией по конечной точке

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Country Link
US (1) US20130102001A1 (https=)
EP (2) EP2768981B1 (https=)
JP (2) JP6258208B2 (https=)
CN (1) CN104011226A (https=)
AR (1) AR088406A1 (https=)
AU (1) AU2012325988B2 (https=)
CA (1) CA2852932A1 (https=)
ES (1) ES2660365T3 (https=)
HK (1) HK1201075A1 (https=)
HU (1) HUE036884T2 (https=)
IN (1) IN2014DN03020A (https=)
NO (1) NO2820231T3 (https=)
PL (1) PL2768981T3 (https=)
PT (1) PT2768981T (https=)
RU (2) RU2639508C2 (https=)
UA (1) UA114302C2 (https=)
WO (1) WO2013059576A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2162642C2 (ru) * 1996-04-26 2001-02-10 Е.И.Дюпон Де Немур Энд Компани Соевое масло с высокой окислительной стабильностью
WO2002036831A2 (en) * 2000-10-30 2002-05-10 Monsanto Technology Llc Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
WO2004072259A2 (en) * 2003-02-11 2004-08-26 Dow Agrosciences Llc Altered fad2 and fad3 genes in brassica and the molecular marker-assisted detection thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5176995A (en) 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
BR0313722A (pt) * 2002-08-12 2007-08-14 Monsanto Technology Llc método para aumentar os nìveis totais de óleos em plantas
EP1837397A1 (en) * 2006-03-21 2007-09-26 Monsanto S.A.S. FAD-2 mutants and high oleic plants
EP2136911A2 (en) * 2007-01-19 2009-12-30 Biodot, Inc. Systems and methods for high speed array printing and hybridization
CN101591697A (zh) * 2008-05-27 2009-12-02 北京华安佛医药研究中心有限公司 一种检测巯基嘌呤甲基转移酶基因a719g多态性位点基因型的试剂盒、方法和用途
US20110151441A1 (en) * 2009-12-18 2011-06-23 Dow Agrosciences Llc Endpoint taqman methods for determining zygosity of corn comprising tc1507 events

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2162642C2 (ru) * 1996-04-26 2001-02-10 Е.И.Дюпон Де Немур Энд Компани Соевое масло с высокой окислительной стабильностью
WO2002036831A2 (en) * 2000-10-30 2002-05-10 Monsanto Technology Llc Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
WO2004072259A2 (en) * 2003-02-11 2004-08-26 Dow Agrosciences Llc Altered fad2 and fad3 genes in brassica and the molecular marker-assisted detection thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BARKLEY N.A. end et.al., A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) Electronic Journal of Biotechnology, 2010, n.13 pp.0717-3458. *
JIN H.J. and et.al., Identification of functional BrFAD2-1 gene encoding microsomal delta-12 fatty acid desaturase from Brassica rapa and development of Brassica napus containing high oleic acid contents, Plant Cell Rep (March 2011) n.30, pp.1881-1892. *
JIN H.J. and et.al., Identification of functional BrFAD2-1 gene encoding microsomal delta-12 fatty acid desaturase from Brassica rapa and development of Brassica napus containing high oleic acid contents, Plant Cell Rep (March 2011) n.30, pp.1881-1892. BARKLEY N.A. end et.al., A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) Electronic Journal of Biotechnology, 2010, n.13 pp.0717-3458. *

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Publication number Publication date
US20130102001A1 (en) 2013-04-25
EP3342881A1 (en) 2018-07-04
UA114302C2 (uk) 2017-05-25
ES2660365T3 (es) 2018-03-22
PL2768981T3 (pl) 2018-07-31
AR088406A1 (es) 2014-05-28
IN2014DN03020A (https=) 2015-05-08
RU2017143585A (ru) 2019-02-15
AU2012325988A1 (en) 2014-05-01
JP2018068306A (ja) 2018-05-10
PT2768981T (pt) 2018-05-18
RU2014120422A (ru) 2015-11-27
NO2820231T3 (https=) 2018-06-16
CN104011226A (zh) 2014-08-27
HUE036884T2 (hu) 2018-08-28
JP2014530623A (ja) 2014-11-20
EP2768981A1 (en) 2014-08-27
JP6258208B2 (ja) 2018-01-10
EP2768981A4 (en) 2015-06-17
CA2852932A1 (en) 2013-04-25
EP2768981B1 (en) 2018-02-14
HK1201075A1 (en) 2015-08-21
WO2013059576A1 (en) 2013-04-25
AU2012325988B2 (en) 2018-04-19

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