RU2622994C2 - Method for human immunity improvment and composition for its implementation - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves inhalation of air enriched with biologically active essential oil solutions sprayed indoors to a concentration of 0.05-0.25 mg/m³. This essential oil contains readily volatile monoterpenes and semi-volatile sesquiterpenes and is obtained by exhaustive hydroparastillation of plant raw materials with water. Essential oil of siberian pine (cedar) containing at least 160 terpenoids or siberian pine (cedar) containing at least 160 terpenoids and siberian fir, containing at least 70 components, taken in the ratio of 1:1, or siberian pine (cedar), containing at least 160 terpenoids, siberian fir, containing at least 70 components, and marsh aura root, containing at least 100 components, taken in the ratio of 1:1:0.1. Also the invention relates to compositions containing the specified essential oil in the amount of 2.5-10% and 95-96% aqueous solution of ethyl alcohol in an amount of 90.0-97.5 wt %.

EFFECT: increased immunity and improved intestinal microflora.

2 cl, 3 tbl, 3 ex

Description

The invention is intended to enhance human immunity and the prevention of diseases transmitted by airborne droplets. The invention can find application in medical institutions, apartments, office premises, kindergartens, schools, educational institutions. In addition, the invention may find application for combating Legionella - parasitic bacteria living in domestic air conditioners.

Chronic inflammatory diseases are often accompanied by immunodeficiency conditions when the immune system is not able to turn on its immune response or responds to a much lesser extent than the body needs to fight the infection.

A modern resident of a large metropolis is daily exposed to various adverse factors that form the environment of his life. In such cases, the fight against sluggish infections is much more difficult. The main danger of infection does not exist in the open air, but in a closed room, where the process of seeding with various forms of pathogenic microbes is much faster, and it is here that the human body becomes the target for fungi, bacteria and viruses of various forms.

In the summer, the main risk group is motorists using a car air conditioner; in enclosed spaces of small volumes, the process of air seeding by pathogenic microflora is several times faster, which means that it is more likely to get sick there.

The fight against legionella (parasites living in air conditioners) in the United States and Japan has been ongoing for 20 years. If it gets into the respiratory tract, the effects of parasitic bacteria cause an unpleasant “tickle” in the nasopharynx, and therefore many refuse to use the air conditioner, especially in cars. We use a variety of disinfectants, but there is a direct sense to replace at least some of them with safe for health - natural. Microbial communities adapt to chemically synthesized preparations, while to complex natural compounds, such as coniferous plant volcanoes, they do not develop resistance.

It is well known that almost all medicinal plants of the Siberian region belong to essential oil plants, that is, they contain in various parts a certain amount of essential oil, the percentage of which can reach up to 5-10%. At the same time, essential oils of many plants have bactericidal and antioxidant properties, contribute to tissue epithelization, therapeutic effect in the treatment of a number of skin diseases, diseases of the musculoskeletal system, bronchopulmonary system and others.

Volatile volatile essential oils are regulators of the physicochemical properties of the air. They lead to an increase in the concentration of light negative ions that have a favorable effect on humans, fulfill the function of “providing” atmospheric air with biologically active oxygen, contribute to the settling of dust particles, create a unique aroma and freshness of air, which favorably affects a person’s emotional state.

Illustrative in this part is the scientific fact, indicating that in the coniferous forest there are about 5 pathogenic microbes in a cubic meter of air, in a city apartment up to 40,000. The same number in school classes, shops, even more in public transport, hospital institutions. In car interiors, this figure is in the millions. Therefore, there is a need to create a new method and composition in the form of a ready-to-use form that would be not only effective, but also easily accessible to the general public.

A known method of treating chronic inflammatory diseases accompanied by immunodeficiency conditions (RU 2522972, IPC A61K 35/14, A61P 43/00). The method involves, after the stage of treatment of the underlying disease and with clinical and outpatient confirmation of the immunodeficiency state, the patient is administered a single-group fresh unrelated cord blood in a volume of three milliliters, mixed with one gram of a broad-spectrum antibiotic and one milliliter of local anesthetic.

The disadvantage of this method is the limited availability of fresh unrelated cord blood, the presence of an antibiotic.

A known method of increasing immunity is that patients determine the blood group and, taking into account the blood group, prescribe a reduced diet with a set of products containing silicon, defined for each blood group (RU 2124360, IPC A61K 35/64). The method allows without the use of drugs to increase the body's resistance to disease, performance.

The disadvantage of this method is the long procedure for increasing immunity, the lack of an accurate assessment, leading to the desired result.

Closest to the claimed method is a method of increasing the adaptive level of the child's body (RU No. 2038092). The method consists in inhaling air enriched in phytoaeroions obtained by dispersion with an aqueous emulsion. Essential oils of star anise, mint, fennel, as well as their mixture with oil of eucalyptus, sage, orange and turpentine at a ratio of 75-85%, are emulsified by 25-15% with distilled water 1: 1000. An electric humidifier is filled with emulsion and after a few minutes the procedure is taken, breathing in the air enriched with phytoaero ions. A course of 25-30 procedures with a session duration of 10-15 minutes, repeating the course 3 times a year.

The disadvantage of this method is the burning of oxygen and the drying of air in the room. For the use of funds require additional training.

Known medicinal and flavored compositions used to saturate indoor air with medicinal substances, the amount of which can be dosed with an ultrasonic aerosol device (RU 2039576, IPC 6 A61L 9/14).

The disadvantages of these compositions are a small spectrum of antimicrobial activity, the absence of a complex effect on the environment, the possibility of an overdose of drugs and the unreliability of the installation used from a fire point of view.

A known mixture of essential oils of conifers, for example, Siberian fir and pine (boron acetate and camphene), taken in the ratio of components 3: 1, with cholinergic action, which can be used in pharmacology for the correction of diseases associated with the disease of the autonomic and central nervous system (RU 2539373 , IPC A61K 36/15, A61P 25/00).

A disadvantage of the known composition is that the technology for the production of essential oils is focused on the production of camphene. The resulting mixture is specifically intended for a narrow spectrum of activity associated with a disease of the nervous system.

The invention solves the single task of enhancing immunity by exposing the pathogenic microflora in enclosed spaces to a biologically active composition in the form of a spray obtained from plant materials, acting as a catalyst of the human immune system and exhibiting its active properties over a long period.

Natural essential oils in the sprays are selected according to the principle of hypoallergenicity, stored in an organic matrix and do not have side effects on the human respiratory system.

A single technical result is to increase immunity and improve intestinal microflora by creating and using a biologically active composition from plant materials in the form of a spray that acts as a catalyst for the human immune system, which has powerful antiviral properties, activates antioxidant protection enzymes (catalase, glutathione reductase) of a person with a prolonged effect actions.

A uniform technical result is achieved in that the method of increasing immunity involves inhaling air enriched with biologically active essential oil of plant origin sprayed in a room, and according to the invention, spraying is carried out in the form of a spray based on a volatile oil containing volatile monoterpenes and hardly volatile sesquiterpenes obtained by exhaustive hydrodistillation of plant materials with water at a temperature of 98-105 ° C, for 30-40 hours and dissolved in a 95-96% aqueous solution et lovogo alcohol to give its concentration in air, 0.05-0.25 mg / m ^3, wherein the essential oil as used Siberian pine essential oil (pine), comprising at least 160 of terpenoids, or Siberian pine (cedar) containing no less than 160 terpenodes, and Siberian fir containing at least 70 components taken in a 1: 1 ratio, or Siberian pine (cedar) containing at least 160 terpenoids, Siberian fir containing at least 70 components, and marsh root containing no less than 100 components taken in a ratio of 1: 1:, 0.1.

The composition for implementing the method of increasing immunity according to claim 1, contains an essential oil from plant materials containing volatile monoterpenes and hardly volatile sesquiterpenes, obtained by exhaustive hydro-steam distillation with water at a temperature of 98-105 ° C, for 30-40 hours, and dissolved in 95-96 % aqueous solution of ethyl alcohol, in the following ratio of components, wt. %:

essential oil 2.5-10.0% 95-96% ethanol aqueous solution 90.0-97.5%,

at the same time, Siberian pine (cedar) essential oil containing at least 160 terpenoids or Siberian pine (cedar) containing at least 160 terpenoids and Siberian fir containing at least 70 components taken in a ratio of 1: 1 are used as essential oil , or Siberian pine (cedar), containing not less than 160 terpenoids, Siberian fir, containing not less than 70 components, and bog root, containing not less than 100 components, taken in the ratio 1: 1:, 0.1.

Obtaining essential oils from plant materials by exhaustive steam distillation for 30-40 hours at a temperature of 98-105 ° C allows you to get essential oil (the know-how of the manufacturer's technology) with a very high content of terpene hydrocarbons, which inactivate viruses, bacteria and fungal spores, inhibit primary forms of clinical infections. So, essential oil of Siberian pine (cedar) contains at least 160 terpenoids, essential oil of Siberian fir contains at least 70 components, essential oil of calamus root contains at least 100 components.

Carrying out the technological process for the production of essential oil for less than 30 hours leads to the release of compounds that are much less active in relation to pathogenic microflora, and more than 40 hours leads to a slowdown in the processes of isolation of biologically active compounds.

The concentration of the essential oil in the composition of less than 2.5% does not have the necessary bactericidal activity. Using a concentration of essential oil of more than 10.0% does not allow spray suspensions to disperse evenly in the air. Recent studies on the production of essential oils of Siberian conifers and on the study of their therapeutic and prophylactic properties have shown that the process of extracting oil components from woody greens stretches over time, which reaches several hours. The study of the component composition of the initial and last fractions of the essential oil of Siberian coniferous plants by chromatography-mass spectrometry showed that the first fractions are enriched with volatile monoterpenes, the latter with hardly volatile sesquiterpenes.

Together with NPO "VECTOR" (Novosibirsk), it was found that the bactericidal activity of these fractions differs markedly with respect to the gram-positive and gram-negative microbial communities of the environment and the gastrointestinal tract of humans, with the last fractions of the essential oil exceeding the first bactericidal factor by 10 times or more . The first fractions of essential oil have increased antioxidant activity, the latter - increased bactericidal activity. Obviously, in order to obtain coniferous essential oil that is most effective on the human body, it is necessary that the oil contains both the first fractions (they are obviously always present) and the last fractions, which are most often absent in many commercial oil samples.

The method of exhaustive hydrodistillation allows the most complete selection of all components during the time necessary for this. In this case, the essential oils contain both volatile monoterpenes and non-volatile sesquiterpenes. Such a composition of essential oil of Siberian pine and Siberian fir turned out to be the most active in the fight against microbial communities of the environment. Given the fact that a mixture of essential oils acts more efficiently, the effectiveness of air sanitation by various fractions of these essential oils was determined. The fractions of the essential oil of Siberian fir and Siberian pine are richer in sesquiterpene hydrocarbons, and, as practice has shown, have a stronger sanitizing effect in relation to the microbial communities of the indoor air environment.

Therefore, a decrease in the number of microbial communities will strengthen the human immune system and reduce the likelihood of catarrhal diseases during sanitation of a room with a mixture of such essential oils.

The resulting composition of essential oils in the form of sprays exhibit a double effect:

- this is an effect on the air (during spraying, pathogenic microflora is suppressed up to 75% per unit volume of air, the effect lasts up to 12 hours);

- spray suspension through the respiratory tract into the blood with penetration into the cell membrane. Terpene hydrocarbons in the composition of essential oils significantly activate the action of antioxidant enzymes, suppressing free radicals and strengthening the human immune system, improving the intestinal microflora (The studies were carried out in collaboration with scientists from the N.V. Sklifosovsky Research Institute of Spirits and the All-Russian Institute of Medicinal Aromatic Plants, Moscow, Research Institute of the Problems of the North (Krasnoyarsk), specialized hospitals and a number of universities in the Siberian Federal District).

As the studies showed, it is precisely such a content of components in the essential oils that shows their increased bactericidal activity for a long period, activating the production of antioxidant enzymes (catalase, glutathione reductase), thereby enhancing the human immune defense system and improving the intestinal microflora (table 1).

The method is as follows.

Essential oil to obtain the composition is obtained from woody greens of plant materials by exhaustive steam distillation for 30-40 hours at a temperature of 98-105 ° C.

The resulting essential oil containing volatile monoterpenes and hardly volatile sesquiterpenes is dissolved in a 95-96% aqueous solution of ethyl alcohol to obtain a composition containing 2.5-10% essential oil and 90.0-97.5% aqueous solution of 95-96% ethyl alcohol.

Then the prepared solution is placed in the dispenser and the essential oil is sprayed in the form of a spray in the room until the concentration of the essential oil in the air is 0.05-0.25 mg / m ³ , producing 5 to 30 sprays by pressing the spray dispenser button, in depending on the volume of the room. This concentration is achieved within 10-15 minutes.

Disinfection of indoor air occurs. The duration of the antibacterial effect of the composition is 6-12 hours, depending on the volume of the room and the number of people in the room.

The patient, being indoors and inhaling a pair of spray compositions, inactivates the pathogens of infection in the upper and lower respiratory tract. The intestinal microflora improves, the immune system activates.

After 6-12 hours, re-disinfect the air.

With an SARS and influenza epidemic, 1 application is needed every hour. Under normal conditions, apply 2-3 times a day.

Essential oils from plant materials for the preparation of sprays have the following properties.

Siberian pine essential oil (cedar)

It is a yellowish-green or bluish-yellow mobile fluid with a characteristic smell of coniferous essential oil.

The oil contains (% wt.):

 alpha pinen  54.0-60.0  beta fellandren  10.0-15.0  delta cadinen  4.0-6.0  beta pinene  2.56-4.5  alpha amorphous  2.0-3.0  episodic  1.0-2.0  alpha muurolen  1.0-2.0

and other terpene compounds.

The number of terpenes and sesquiterpenes in the component composition of the essential oil should be at least 160.

Key physical and chemical indicators

the density of the essential oil of Siberian pine is 0.8600-0.8850 g / cm ³ ,

- refractive index - 1.4680-1.4750.

Siberian fir essential oil

It is a clear or slightly yellowish mobile fluid with a characteristic smell of coniferous essential oil

Essential oil from woody green Siberian fir has a density of 0.9020-0.9120 g / cm ³ , the refractive index of 1.4650-1.4730.

The component composition of Siberian fir essential oil consists of more than 70 components, the main of which are (% wt.):

bornyl acetate 30.0-40.0 camphin 15.0-20.0 alpha terpinene 5.0-8.0 alpha pinen 4.0-7.0 limonene 4.0-6.0 trans caryophyllene 1.0-3.0 alpha bisabolol 1.0-3.0 alpha caryophyllene 0.5-2.0

The main physico-chemical characteristics of the resulting product

- density - 0.830-0.840 g / cm ³ ,

- refractive index - 1,340-1,360,

- acid number is less than 0.05 mg KOH / g,

- indicator of acidity (pH) - 6.5-7.0.

Essential oil of calamus root

Color from bluish-yellow to brown, mobile liquid with a bright pleasant spicy smell.

Essential oil of calamus root bog, contains more than 100 components, the main of which are (% wt.):

 acorenone 12.0-14.5  camphin 8.0-10.5  terpenyl butonate 8.0-10.5  alpha + delta selinen 8.5-10.5  camphor 6.0-8.0  isocalamendiol 3.0-4.5

The main physical and chemical parameters of the resulting product:

- density - 0.830-0.840 g / cm ³ ,

- refractive index - 1,340-1,360,

- acid number is less than 0.05 mg KOH / g,

- indicator of acidity (pH) - 6.5-7.0.

Example 1. Obtaining a spray from the essential oil of woody greens of Siberian pine (cedar).

Siberian pine essential oil is obtained under conditions of exhaustive hydro-steam distillation of fresh woody greens (paws) of Siberian pine for 36 hours at a temperature of 98 ° C. It is a yellowish-green mobile fluid with a characteristic smell of coniferous essential oil. The density of the Siberian pine essential oil is 0.8700 g / cm ³ , the refractive index is 1.4730. The oil contains (% wt.):

 alpha pinen  56.0  beta fellandren  12.0  delta cadinen  5,0  beta pinene  3.8  alpha amorphous  2.7  episodic  1,6  alpha muurolen  1.4

and other terpene compounds.

The number of individual compounds in essential oil is 160.

Essential oil containing volatile monoterpenes and hardly volatile sesquiterpenes, in the amount of 2.5 g (2.5%) is dissolved in 97.5 g (97.5%) of a 96% aqueous solution of ethyl alcohol. The resulting solution is placed in a spray tank. Spray the spray indoors for 15 minutes. The concentration of essential oil in the air is 0.05 mg / m ³ . The bactericidal effect of the sprayed drug lasts for 10 hours.

Example 2

Analogously to example 1. The essential oil of Siberian pine (cedar) and Siberian fir is obtained under conditions of exhaustive hydrodistillation of fresh woody greens (paws) of Siberian pine and Siberian fir for 40 hours at a temperature of 101 ° C. The resulting Siberian pine essential oil contains 162 components, Siberian fir essential oil consists of 73 components.

Take 5.0 g (5%) of Siberian pine essential oil and 5.0 g (5%) of Siberian fir essential oil. The oil mixture is dissolved in 90.0 g (90%) of a 95% aqueous solution of ethyl alcohol. The resulting solution is placed in a spray tank. Spray the spray indoors for 15 minutes. The concentration of essential oil in the air is 0.25 mg / m ³ . The bactericidal effect of the sprayed drug lasts for 12 hours.

Example 3

Analogously to example 1. Essential oil of Siberian pine (cedar), Siberian fir and calamus root are obtained under the conditions of exhaustive steam distillation of fresh woody greens (paws) of Siberian pine and Siberian fir for 40 hours at a temperature of 105 ° C. The obtained essential oil of Siberian pine contains 161 components, the essential oil of Siberian fir consists of 70 components, the essential oil of calamus root contains 100 components.

Take 3.0 g (3%) of Siberian pine essential oil, 3.0 g (3%) of Siberian fir essential oil and 0.3 g (0.3%) of essential oil of calamus root. The oil mixture was dissolved in 93.7 g (93.7%) of a 96% aqueous solution of ethyl alcohol. The resulting solution is placed in a spray tank. Spray the spray indoors for 15 minutes. The concentration of essential oil in the air is 0.20 mg / m ³ . The bactericidal effect of the sprayed drug lasts for 12 hours.

The composition of the essential oil and the finished composition was established using an Agilent Technologies 7890 GC System gas chromatography mass spectrometer with a 5975 C quadrupole mass spectrometer as a detector using a 30 m capillary column with a phase of 5% diphenyl-95% dimethylsiloxane with an inner diameter of 0 , 25 mm. Chromatography conditions: isothermal at 50 ° C for three minutes, then a programmed temperature rise at a speed of 4 ° C / minute to 270 ° C with exposure at a final temperature of 30 minutes. The evaporator temperature is 280 ° C, the temperature of the ionization chamber is 170 ° C, and the ionization energy is 70 eV. The content of the components is calculated by peak areas, the identification of individual components is carried out by comparing the retention times and total mass spectra with the corresponding data of the components of the reference oils and pure compounds, as well as using linear retention indices. The composition of the essential oil of calamus root provides a high bacteriostatic, fungistatic and anti-inflammatory activity, complements the component composition of the Siberian pine and Siberian fir essential oils with other components, which complicates the habituation of environmental microorganisms and contributes to the prolongation of the bactericidal action of such compositions.

The bactericidal activity of the obtained products was studied by the method of serial dilutions. The degree of the test drug was judged by its minimum concentration, which causes a complete suppression of dehydrogenases of test culture cells after 1 hour of incubation in an incubator at a temperature of 37 ° C compared with the control. The multiplicity of dilutions was taken equal to two.

The conditionally pathogenic microorganisms of the Enterobacteriacea family were selected as test objects: Enterobacter cloacea, Proteus vulgaris, Escherichia coli, Citrobacter freundii, Pseudomonas aeroginosa, Klebsiella pneumoniae. These microorganisms are one of the most common in the environment and are sanitary-indicative. The research results are shown in table 2.

To study the sanitation properties of essential oils, the Koch sedimentation method was used. The microbial number of air was calculated according to the Omelyansky rule. For the rehabilitation of the premises used the concentration of the preparations obtained in examples 1-3 in the range of 0.020-0.030 mg / m ³ . Samples for the study were taken at a distance of 1 m from the floor to rehabilitation, then 15 minutes, 1, 2, 3, 4 and 12 hours after rehabilitation. The results of the study are shown in table 3.

A research method for studying the clinical effectiveness of the use of a bactericidal spray (bactericidal sprays) in complex treatment was tested on the basis of the Krasnoyarsk Regional TB Dispensary No. 2 and the establishment of IK-22 (female general correctional colony).

Studies were conducted on 2 groups of the subjects:

The first group of patients was patients with newly diagnosed forms of tuberculosis, representing an epidemiological danger.

The research methodology is based on aeration (spraying) of the bactericidal spray 3 times a day in the main group (12 people) and the corresponding control group (12 people). The duration of the study is 3-4 months.

Evaluation of the effectiveness of treatment was carried out by highly effective diagnosis of the intensity of bacterial excretion using the automated system Bactec MGIT960 with a time interval after 1, 2, 3 months of treatment (2 times a month).

The results of studies on liquid nutrient media showed that after 3 months of daily aeration of the room with a bactericidal spray in the ward of patients of the main group, the MBT growth rate from 3+ decreased to 1+, while in the control group of patients it remained at the same level. The research data showed that gram-negative flora (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus) was inactivated in the liquid nutrient media of patients of the main group, the growth rate and the rate of bacterial excretion decreased. In the main group of patients, bacillus growth in the studied media was observed on the 18th day, in the control - on the 4th. In the main group of patients, no complicated local forms of primary tuberculosis were found, while in the control group 2 cases of complications were revealed.

2 group of examinees - conditionally healthy, able-bodied age.

The research methodology is based on aeration of the bactericidal spray 3 times a day in the main group (12 people) and the corresponding control group (12 people). The duration of the study is 22 days. Frequency of measurements 1 time in 3 days: 1st sowing - before treatment of the room with a spray composition; 2nd sowing - 15 minutes after aeration of the room with a spray composition; 3rd sowing - 4 hours after processing the premises.

Evaluation of the effectiveness of treatment: a sanitary analysis of air culture for pathogenic flora according to the microbial number - the number of individuals found in 1 m ^{3 of} air.

The results of the studies showed that air sowing before treatment on average contained 18,000 pathogenic microbes in 1 m ³ , after treatment it was about 4000. Sowing taken after 4 hours after processing the room had a value of about 3500 individuals per 1 m ³ . In the air of the premises of the main group of patients with respect to the control, a decrease in the spectrum of pathogens of the bacterial flora and a decrease in its intensity was revealed, as a result - a decrease in the threshold of incidence.

In both main groups of patients, an improvement in intestinal microflora was noted.

Studies have shown an increase in the effectiveness of treatment of tuberculosis patients with the combined use of etiotron therapy of tuberculosis and pathogenetic treatment methods.

The composition of sprays used in the studies based on essential oils of Siberian pine pine has a bactericidal effect of a double action: 1 - reduces the microbial contamination of air; 2 - inactivates the microflora of the respiratory tract, enhancing the anti-inflammatory effect against the background of the treatment received for non-specific respiratory diseases present in patients with tuberculosis, stimulates the body's immune system, improves the intestinal microflora, and as a result, accelerates reparative processes, increases the body's resistance to viral and bacterial infections in conditionally healthy group of studied patients.

Conducting regular sanitation of classrooms for first-year students of Siberian Federal University during November-December 2014 showed that such processing of premises leads to an increase in students' working capacity, better assimilation of educational material, and a decrease in the incidence of SARS.

As a result of using the present invention, it becomes possible to expand the range of environmentally friendly highly effective fungicidal, antimicrobial and antiviral compositions based on natural phytoextract compounds with pronounced inactivating activity against pathogenic microorganisms (viruses, bacteria, fungi) and at the same time possessing a general strengthening and adaptogenic effect on the body human, and provides air purification in the most environmentally friendly way. The use of the proposed composition provides the treatment and prevention of microbial etiology and closed space infections by physiological methods - exposure to pathogenic forms of microorganisms (bacteria, viruses and fungi, including molds) while enhancing the protective properties of the human body.

The proposed product is a natural and safe product that shows an excellent pharmacotherapeutic effect, and is recommended by leading medical specialists, including women during lactation and pregnancy. It is this group that remains the most unprotected, as it has limitations in taking medications. When using sprays for infants by spraying in the bedside zone, they became calmer, normalized sleep, improved overall health, symptoms of colds and intestinal infections passed. When inhaled spray compositions after 15 minutes, the antioxidants of essential oils enter the bloodstream. Recent studies show that the proposed spray compositions are an excellent anti-stress drug.

When using the proposed composition for cleaning air conditioners, the destruction of legionella - parasitic bacteria occurs. Legionella - colonies of parasitic bacteria living in air conditioners love human lung tissue very much, having a pronounced attachment to it. It is there that the pathogen finds favorable conditions for reproduction and causes severe pneumonia, which can lead to death. Legionella can live in the aquatic environment for up to a year, while in 70% alcohol they die within 1 minute. Studies show that colonies of these parasitic bacteria do not develop resistance to a spray composition based on coniferous essential oils with a high concentration of terpene hydrocarbons, and alcohol suspensions in the composition enhance the bacteriostatic effect.

The proposed spray based on hypoallergenic essential oils with pronounced bactericidal properties can be used as a year-round means of prevention and protection against pathogenic microbes in indoor air and make air conditioners comfortable to use.

Claims (4)

1. A method of increasing immunity, including inhaling air enriched with solutions of biologically active essential oil sprayed in a room, characterized in that they are sprayed in the form of a spray of essential oil containing highly volatile monoterpenes and non-volatile sesquiterpenes obtained by exhaustively hydro-steam distillation of plant material with water at a temperature of 98 -105 ° C, for 30-40 hours, and dissolved in a 95-96% aqueous solution of ethyl alcohol, with a concentration in the air of 0.05-0.25 mg / m ³ , while in quality We use essential oil of Siberian pine (cedar), containing at least 160 terpenoids, or Siberian pine (cedar), containing at least 160 terpenoids, and Siberian fir, containing at least 70 components taken in a 1: 1 ratio, or pine Siberian (cedar), containing not less than 160 terpenoids, Siberian fir, containing not less than 70 components, and marsh calf root, containing not less than 100 components, taken in the ratio 1: 1: 0.1. 2. The composition for implementing the method of enhancing immunity according to claim 1, containing essential oil from plant materials, characterized in that the essential oil is an essential oil containing volatile monoterpenes and non-volatile sesquiterpenes in a 95-96% aqueous solution of ethyl alcohol, obtained by exhaustive hydrodistillation with water at a temperature of 98-105 ° C, for 30-40 hours, and dissolved in a 95-96% aqueous solution of ethyl alcohol, in the following ratio, wt.%: essential oil 2.5-10.0% 95-96% ethanol aqueous solution 90.0-97.5% at the same time, Siberian pine (cedar) essential oil containing at least 160 terpenoids or Siberian pine (cedar) containing at least 160 terpenoids and Siberian fir containing at least 70 components taken in a 1: 1 ratio are used as essential oil , or Siberian pine (cedar), containing at least 160 terpenoids, Siberian fir, containing at least 70 components, and marsh root, containing at least 100 components, taken in a ratio of 1: 1: 0.1.