RU2609906C1 - Method for determining probable ovulation in cows - Google Patents

Abstract

FIELD: agriculture; veterinary medicine.

SUBSTANCE: method for determining the optimal time of ovulation in cows includes the study of mucus, at that the mucus taken with a cottonwool stick from the oral cavity, a smear is made and placed on a glass slide, air dried, examined under a microscope at 100-fold magnification, the ovulation is diagnosed by the presence of crystalline ferny pattern.

EFFECT: use of the invention enables to determine ovulation in cows.

1 tbl, 2 dwg

Description

The invention relates to veterinary medicine, in particular to veterinary obstetrics, and can be used for rapid diagnosis of saliva of the sexual cycle in the reproductive system of cows.

A known method for determining the functional state of the ovaries in farm animals, including taking smears from the walls of the upper lateral vaginal fornix, counting the percentage of certain types of surface epithelial cells and judging the phases of the reproductive cycle and the functional state of the ovaries in cows, is preliminary, starting from 25-30 day of the postpartum period, the content of total cholesterol and the indicators of the albumin-globulin coefficient of blood are examined, then in animals with a total content of cholesterol at the level of 5.3-7.5 mmol / l and having an albumin-globulin coefficient in the range of 1.0-2.2, which exhibit a persistent reflex of immobility, additionally determine the pH and refractometric index of refraction of the cervico-vaginal secretion and at a pH of 5.0-6.8 and a refractive index of refraction of cervico-vaginal secretion equal to 1.3342-1.3352, as well as with the content of self-surface cells with a clear nucleus on vaginal smears up to 7.5% diagnose the presence of functionally active follicle with a mature egg in the ovaries of cycling cows and judge about favorable conditions for fertilization. (RF patent No. 2211683, publ. 09/10/2003).

A known method for predicting the fertility of cows, including a study of cervical mucus, characterized in that in order to increase the efficiency of insemination of cows, a 0.02% solution of furatsilin is added to a mixture of cervical mucus and 4% sodium hydroxide heated to a boil and in the presence of a stable light - red or pink coloration of the mixture predict the highest fertility of cows. A 0.02% solution of furatsilin contribute in a volume equal to the volume of cervical mucus. (RF patent No. 2075966, publ. 03/27/1997).

A known method for determining the ovulation period, including contact of the mucous secretions (CB) with the electrodes of the sensor and subsequent measurement during the menstrual cycle of the electrical parameter and its maximum value, determining the ovulation period, characterized in that they measure the polar conductivity of CB, which allows indirectly determine the concentration of luteinizing hormone in the blood, and the maximum value of the polar conductivity determines the period of ovulation. (RF patent No. 2180443, publ. 03/10/2003).

A known method of determining ovulation in a systematic rectal examination of the ovaries. At the beginning of the hunt, the preovulatory follicle is about 1 cm in diameter. It has an elastic-elastic consistency, by the time of ovulation it clearly fluctuates and reaches a size of 2-2.5 cm. After ovulation in place of a previously fluctuating follicle, a depression is easily determined (as a result, the size of the ovary decreases). After 6-8 hours, the fossa at the site of the ovulated follicle is difficult to determine due to the formation of a blood clot. (http://chitalky.ru/?p=4886).

Known progesterone test for determining ovulation in cows. The method is based on multiple measurements of the concentration of progesterone in plasma of peripheral blood or milk. In heifers, blood serves as a material for analysis, in cows - milk. Samples for analysis are taken every other day or once every 2-3 days and examined in a special laboratory. The concentration of the hormone is measured by radioimmunological or enzymeimmunological method. The insemination time of cows and heifers is determined by the rate of progesterone falling to the lowest (basal) level, which is only a likely sign of hunting. It occurs 50 hours before ovulation (approximately 20-24 hours before the start of the hunt). To diagnose one stage of arousal, 9-11 studies are needed. Using this test, it is impossible to diagnose the first stage after the birth of the excitation of the sexual cycle, as well as the stage of excitation, which manifests itself in the anovulatory sexual cycle. (http://chitalky.ru/?p=4886).

The phenomenon of arborization of cervical mucus is known. A diagnostic sign is a symptom of a “fern leaf”. It is due to the crystallization of salts of cervical mucus. The severity of this phenomenon is directly proportional to estrogenic activity and reaches a maximum at the time of ovulation. After ovulation, the “fern leaf” begins to break down into separate fragments and acquires an amorphous form during the heyday of the corpus luteum. (http://chitalky.ru/?p=4886)

The disadvantages of the known methods for determining ovulation in cows are:

- the procedure for taking cervical mucus is quite complicated, inefficient and involves a certain risk of injury and infection of the genital organs;

- rectal diagnosis of ovulation is time-consuming, requires practical skills for the differential diagnosis of ovarian diseases (ovarian cysts, persistent corpus luteum);

- when conducting a progesterone test, it takes time for a laboratory study of blood for hormones and their interpretation, as well as material costs.

The task is to simplify the diagnosis of the most probable period of ovulation and establish the optimal time for insemination of cows.

The technical result is the determination of the timing of probable ovulation in cows.

The specified technical result is achieved by the method of determining the optimal ovulation period in cows, including the study of mucus, while the mucus is taken with a cotton swab from the oral cavity, a smear is made and placed on a glass slide, dried in air; examined under a microscope at a 100-fold increase, ovulation is diagnosed by the presence of a crystalline fern-like pattern.

The hormonal state of a cow is characterized by the correct ratio of sex hormones at different stages of the reproductive cycle. In the first half of the cycle, the amount of estrogen hormones increases and reaches a maximum on the day preceding the release of a mature egg - ovulation.

Estrogen stimulation causes salivation with an increased amount of sodium chloride, the concentration of which increases as much as possible on the day of ovulation. An increase in the concentration of salt in saliva leads to its crystallization, which begins to appear in a healthy cow several days before the day of ovulation, reaching a maximum on the day of ovulation (this day corresponds to the maximum severity of crystallization - the appearance of “fern leaves”).

By analyzing under a microscope the change in the crystallization pattern of dried saliva, it is possible to determine the probable time of ovulation (which contributes to a favorable conception) and, accordingly, increase the likelihood of pregnancy of cows.

An example of a specific implementation.

Production experience was carried out from June to August 2014 in the SEC. Kirov of the Staropoltavsky district of the Volgograd region on heifers 15-16 months of age of Holstein-Friesian breed, ready for insemination, selected according to the analogue principle. Sexual hunting was determined by observing the animals in the section during the morning round (for anxiety) and the following visible signs: the labia is swollen, during vaginal examination, the vaginal mucosa is hyperemic, the cervical canal is relaxed, and mucus leakage has been noted in many animals - estrus. With rectal examination, the uterus is enlarged, shows rigidity well. As sexual hunting was identified, two groups were formed.

The first experimental group was subjected to a saliva smear with a cotton swab from the oral cavity in cows. They made a smear and placed it on a glass slide. Dried in air until completely dry. Examined under a microscope at 100x magnification. Ovulation was diagnosed by the presence of crystalline fern-like pattern.

Heifers whose smear smears showed an obvious fern pattern were seeded with rectocervical method in the evening, once, in which it was less pronounced - in the morning of the next day. (Fig. 1).

In saliva smears, heifers in which crystallization was absent were transferred to the group for further observation. (Fig. 2).

The second group, the control, was artificially inseminated in the presence of signs of sexual hunting, without taking a smear of saliva.

The data are given in the table.

As a result of the data obtained, we see that the determination of probable ovulation in heifers ready for insemination using the test for crystallization of saliva increased the percentage of fertility by 15%.

Therefore, in addition to identifying sexual hunting, the determination of the probable time of ovulation by the degree of crystallization of the animal’s saliva allowed insemination to be carried out at the optimum time of the sexual cycle.

Thus, a method for determining the optimal period of ovulation in heifers, including the study of mucus, while the mucus is taken with a cotton swab from the oral cavity, a smear is made on a glass slide, placed on a glass slide, dried in air; examined under a microscope at a 100-fold increase, ovulation is diagnosed by the presence of a crystalline fern-like pattern, which allows you to determine the timing of probable ovulation in heifers and cows.

Claims (1)

A method for determining the optimal period of ovulation in cows, including the study of mucus, characterized in that the mucus is taken with a cotton swab from the oral cavity, a smear is made and placed on a glass slide, dried in air; examined under a microscope at a 100-fold increase, ovulation is diagnosed by the presence of a crystalline fern-like pattern.

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